<h3><strong>A Dedicated Solution for Oligonucleotide Analysis</strong></h3><p>Oligonucleotides have become key players in modern pharmaceutical and biological research, particularly in the development of highly targeted and personalized therapies. As increasing numbers of oligonucleotide-based drugs enter clinical trials or receive regulatory approval, analytical laboratories face growing demands for both throughput and data quality. These molecules present unique chromatographic challenges due to their long, highly polar structures and subtle variations in nucleobase sequence or functionalization.</p><h3><strong>Sepapure oliGO – Designed for Oligonucleotide Separations</strong></h3><p>Selecting the optimal chromatographic approach for oligonucleotide analysis can be complex. Many laboratories rely on ion-pair reversed-phase (IP-RP) chromatography because of its speed, robustness, and compatibility with mass spectrometry. However, commonly used ion-pair reagents can degrade conventional silica-based stationary phases.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/columns-oligonucleotide-columns"><strong>Sepapure oliGO</strong></a> addresses this limitation through a hybrid silica matrix combined with specialized surface end-capping, ensuring resistance to aggressive IP-RP reagents. Its functionalized surface enables both strong reversed-phase interactions and controlled ionic contributions, effectively creating a mixed-mode separation mechanism. This design results in improved durability compared with standard mixed-mode columns, making Sepapure oliGO a robust choice for routine oligonucleotide workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg" alt="KNAUER: Figure 1 - Sepapure oliGO columns with laser-etched column information." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg 223w," sizes="100vw" width="1186" height="828"></p><h3><strong>Method Transfer and Comparative Performance</strong></h3><p>In collaboration with BianoGMP GmbH, KNAUER’s application team evaluated Sepapure oliGO against a widely used reference column. The study compared oligonucleotides of varying lengths and purities, including a fully thiolated 43-mer DNA. Particular attention was paid to potential interactions between thiolated oligonucleotides and stainless-steel hardware, which have been reported to cause peak tailing.</p><p>The results demonstrated that direct method transfer between the reference column and Sepapure oliGO was generally feasible without significant adjustment. Chromatographic resolution remained comparable, while retention times were slightly shorter on Sepapure oliGO. Furthermore, the anticipated benefits of expensive bioinert hardware were not confirmed under the tested conditions, suggesting that such effects may primarily influence very fast (U)HPLC separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg" alt="KNAUER: Figure 2: Overlayed chromatogram of crude product of a 43mer thiolate oligonucleotide. Light blue - reference column and dark blue – Sepapure oliGO." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg 245w," sizes="100vw" width="1223" height="453"></p><h3><strong>Low Backpressure for Reliable Operation</strong></h3><p>Sepapure oliGO can be applied effectively in both HPLC and (U)HPLC workflows. Its low operating backpressure minimizes risks associated with clogging, excessive system wear, and performance deterioration over time. Typical IP-RP separations using 3 µm particles generated pressures around 200 bar, enabling laboratories without ultra-high-pressure systems to perform high-quality oligonucleotide analysis.</p><h3><strong>Best Practices for Column Performance</strong></h3><p>To maintain optimal performance, users should observe recommended operating conditions, including a broad <strong>pH range of 1.5–10</strong> and <strong>temperatures up to 60 °C.</strong> Employing a <strong>guard column</strong> can prevent coarse synthesis by-products from reaching the analytical column. If pressure increases are observed, backflushing at low flow rates can restore performance.</p><p>When the system is idle, thorough flushing to remove salts is essential. For storage, the column should be kept in a <strong>1:1 mixture of methanol–water</strong> or <strong>acetonitrile–water.</strong> Gradient transitions are recommended when switching between standard RP eluents and IP-RP reagents. Regeneration procedures developed for RP columns are also suitable for Sepapure oliGO.</p><h3><strong>Beyond Analytical Separations</strong></h3><p>For large-scale purification, ion-exchange chromatography (AEX) may offer advantages. Scalable preparative systems are available to support purification workflows, ranging from compact laboratory setups to pilot-scale platforms with flow rates up to 800 mL/min. Bioinert configurations can be integrated where required, allowing laboratories to tailor purification solutions to their specific oligonucleotide applications.</p><blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <strong>sales@knauer.net</strong>. Stay tuned for more exciting insights into the Oligonucleotide world in our “<strong>Oligos Made Easy</strong>” series.</li><li>For more in-depth discussion or questions, reach out to the author at <strong>folmert@knauer.net&nbsp;</strong></li></ul></blockquote>

Oligos Made Easy - Part 8: Sepapure oliGO

<p><strong>Visit the LabRulez booth at </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871"><strong>analytica 2026</strong></a><strong> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"></p><h3><strong>Would you like to learn how the LabRulez portals work?</strong></h3><p>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/"><strong>LabRulezLCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/"><strong>LabRulezGCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/"><strong>LabRulezICPMS</strong></a></p><ul><li>A <strong>unique online solution</strong> for <strong>your marketing</strong></li><li>A <strong>targeted audience</strong> from the <strong>analytical chemistry community</strong></li><li><strong>Modern technologies</strong> and a unique concept</li><li><strong>Design optimized for most smart devices</strong> – PC, mobile, and tablet</li><li><strong>Advanced statistical tools</strong> fully available to users</li></ul><h3><strong>Services Offered</strong></h3><h4><strong>News + Company Updates</strong></h4><ul><li>Publish <strong>news related to your instrumentation, applications, services, events, job openings</strong>, and more</li><li><strong>News is shared</strong> both on our <strong>portals</strong> and across our <strong>social media channels</strong></li></ul><h4><strong>Library – All Documents in One Place</strong></h4><ul><li><strong>Applications, posters, presentations, technical articles, brochures, specifications</strong></li><li><strong>Fas</strong>t and <strong>advanced search</strong> capabilities</li><li>Over <strong>32,000 documents</strong> currently available in our database</li></ul><h4><strong>Webinars</strong></h4><ul><li><strong>Upcoming and on-demand webinars</strong> from the field of analytical chemistry</li><li>More than <strong>5,500 webinars</strong> covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4><strong>Careers</strong></h4><ul><li><strong>Targeted job</strong> listings in <strong>analytical chemistry</strong></li><li>Clear filtering by specialization or location</li><li><strong>Increased visibility for job postings</strong></li></ul><h4><strong>Companies</strong></h4><ul><li><strong>Company profile</strong> with key information and contacts</li><li><strong>Integration of all your activities on our portal:</strong> News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li><strong>Connection to your Products </strong>listed on LabRulez portals</li></ul><h4><strong>Products and Services</strong></h4><ul><li>Comprehensive <strong>overview of instrumental techniques and services</strong></li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4><strong>Marketplace</strong></h4><ul><li><strong>Buying and selling pre-owned analytical equipment</strong></li><li>We purchase analytical instrumentation of all brands and ages</li><li><strong>Sell your equipment through LabRulez</strong></li><li>Request and purchase <strong>complete systems, modules</strong>, or <strong>spare parts</strong> with warranty and service</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"></p><h3><strong>What Will Be on Display at Our Booth?</strong></h3><p>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar"><strong>LabRulezLCMS Marketplace</strong></a></h4><p><strong>Spare parts for LC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378"><strong>Vacuum turbo pumps for LC/MS</strong></a></li></ul><p><strong>Spare parts for HPLC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397"><strong>Degassers for HPLC/UHPLC</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve"><strong>Automatic valves for HPLC</strong></a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377"><strong>HPLC lamps</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48"><strong>HPLC columns</strong></a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar"><strong>LabRulezGCMS Marketplace</strong></a></h4><p><strong>Spare parts for GC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo"><strong>Turbo pumps for GC/MS</strong></a></li></ul><p><strong>Spare parts for GC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC"><strong>EPC modules</strong></a></li></ul><p><strong>Sample injection</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432"><strong>Autosampler 7693A for GC</strong></a></li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7"><strong>GC columns </strong></a>and selected accessories</li></ul>

LabRulez at analytica 2026

News from LabRulezLCMS Library - Week 13, 2026

Article

Product

Events

Application

Science and research

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

ECOM ECS06 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).</strong></p><p>The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning <strong>up to 100 Hz</strong>. The units are communicated through <strong>RS232</strong> or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> from installation.</p><p>A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.</p><h3><strong>Main parts</strong></h3><h5><strong>ECDA2800 UV-VIS PDA DETECTOR</strong></h5><p>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.</p><h5><strong>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</strong></h5><p>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.</p><h5><strong>ECP2010 ANALYTICAL HPLC PUMP</strong></h5><p>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 40 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.</p><h5><strong>ECB2004B GRADIENT BOX WITH DEGASSER</strong></h5><p>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.</p><h5><strong>AUTOSAMPLER AS96</strong></h5><p>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. For 2x48 vials, max. pressure 40 MPa (5801 psi)</p><h5><strong>CLARITY CHROMATOGRAPHY SOFTWARE</strong></h5><p>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.</p><h3>SYSTEM CONTAINS&nbsp;</h3><p><strong>Pcs / Description (P/N)</strong></p><ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010 Analytical HPLC pump (ACA0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 40 MPa, with or without heating and cooling module (ASA97010/ASA97000)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul><p>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> requirements..</p><h3>Configuration options</h3><ul><li><strong>ASA0600X</strong> - Gradient analytical system - incl. autosampler <strong>without cooling and heating</strong>, up to 40 MPa (5801 psi)</li><li><strong>ASA06C0X</strong> - Gradient analytical system – incl. autosampler <strong>with cooling and heating</strong>, up to 40 MPa (5801 psi)</li></ul></div>

ECOM ECS07 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump (60 MPa, 8702 psi), a gradient box, and an autosampler.

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 60 MPa (8702 psi).</strong></p><p>The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning <strong>up to 100 Hz</strong>. The units are communicated through <strong>RS232</strong> or <strong>Ethernet (LAN)</strong>. An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> from installation.</p><p>A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.</p><h3><strong>Main parts</strong></h3><h5><strong>ECDA2800 UV-VIS PDA DETECTOR</strong></h5><p>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.</p><h5><strong>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</strong></h5><p>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.&nbsp;</p><h5><strong>ECP2010H ANALYTICAL HPLC PUMP</strong></h5><p>This isocratic pump works with Gradient Box ECB2004B as a <strong>quaternary gradient pump</strong>. The flow rate range is <strong>0.001-10.00 ml/min</strong> and the pressure limit is <strong>60 MPa</strong>. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.</p><h5><strong>ECB2004B GRADIENT BOX WITH DEGASSER</strong></h5><p>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.</p><h5><strong>AUTOSAMPLER AS96</strong></h5><p>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in <strong>variants with or without a cooling and heating module</strong>. State-of-the-art injection technology with fast injection and wash cycles. Max. pressure <strong>60 MPa.</strong></p><h5><strong>CLARITY CHROMATOGRAPHY SOFTWARE</strong></h5><p>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.</p><h3>SYSTEM CONTAINS&nbsp;</h3><p><strong>Pcs / Description (P/N)</strong></p><ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010H Analytical HPLC pump (ACF0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 60 MPa, with or without heating and cooling module (ASA96000/ASA96010)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul><p>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> requirements..</p><h3>Configuration options</h3><ul><li><strong>ASA0700X</strong> - Gradient analytical system - incl. autosampler <strong>without cooling and heating</strong>, up to 60 MPa.</li><li><strong>ASA07C0X </strong>- Gradient analytical system – incl. autosampler <strong>with cooling and heating</strong>, up to 60 MPa.</li></ul></div>

ECOM LED20M FIX Dual UV Detector with display

The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0"><p>The <strong>LED20M FIX Dual UV Detector</strong> with a display uses <strong>Light Emitting Diodes (LED) </strong>as the light source. It is used for flash chromatography.</p><h3><strong>Why choose LED20M FIX detectors for your application</strong></h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The <strong>optical flow cell </strong>is <strong>easily accessible </strong>and <strong>replaceable</strong>, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to <strong>low power dissipation of LEDs</strong> makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the <strong>integrated OLED display </strong>in <strong>numerical and graphical formats</strong>.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h4><strong>Wavelengths</strong></h4><ul><li>The dual UV detector version measures at two fixed wavelengths, which are adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 and 280 nm are used. Optionally, the customer can select a wavelength in the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h4><strong>Control</strong></h4><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with <strong>ECOMAC SW</strong>, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h4><strong>Key features and benefits</strong></h4><ul><li><strong>OLED display</strong></li><li><strong>Numerical and graphical view mode</strong></li><li><strong>Long lifetime</strong> of the <strong>LED</strong> light source</li><li><strong>Low cell heating </strong>due to <strong>low power losses of the LEDs</strong></li><li><strong>USB C</strong> powering and communication port</li><li><strong>ECOMAC Basic software for free</strong></li><li><strong>Autozero</strong> function</li><li><strong>Easy flow cell replacement</strong></li><li>Small dimensions</li><li><strong>ECOMAC SW </strong>- &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul></div>

ECOM LED20M FIX Single UV Detector with display

The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0"><p>The <strong>LED20M FIX Single UV Detector</strong> with a display uses <strong>Light Emitting Diodes (LED) </strong>as the light source. It is used for flash chromatography.</p><h3><strong>Why choose LED20M FIX detectors for your application</strong></h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The <strong>optical flow cell </strong>is <strong>easily accessible </strong>and <strong>replaceable</strong>, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to <strong>low power dissipation of LEDs</strong> makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the <strong>integrated OLED display </strong>in <strong>numerical and graphical formats</strong>.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h3><strong>Wavelengths</strong></h3><ul><li>The single UV detector version measures at 1 fixed wavelength, which is adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 or 280 nm are used. Optionally, customers can select a wavelength within the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h3><strong>Control</strong></h3><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with <strong>ECOMAC SW</strong>, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h3><strong>Key features and benefits</strong></h3><ul><li><strong>Practical OLED display</strong></li><li><strong>Numerical and graphical view mode</strong></li><li><strong>Long lifetime</strong> of the <strong>LED</strong> light source</li><li><strong>Low cell heating </strong>due to <strong>low power losses of the LEDs</strong></li><li><strong>USB -C</strong> powering and communication port</li><li><strong>Autozero</strong> function</li><li><strong>Easy flow cell replacement</strong></li><li>Small dimensions</li><li><strong>ECOMAC SW </strong>- &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul><p><strong>Note:</strong> A preparative cell PFC 200 Basic is supplied with the unit. Other flow cells are available at request.</p></div>

ECOM LED20G Dual UV Detector

LED20G Dual UV detector for preparative and flash chromatography. ECOM's unique patented technology radically improves the detectors´ features. LEDs are used as the source of light.

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>LED20G Dual UV LED detector for preparative and flash liquid chromatography.</strong> LEDs (Light Emitting Diodes) are used as the light source. <strong>A unique and patented ECOM technology radically improves the detector characteristics.&nbsp;</strong></p><p>The LEDs themselves typically have a peak wavelength with a tolerance of +/- 5 nm and a spectral half-width of 10-20 nm, which is unacceptable for some applications, as e.g. for FPLC. This ECOM solution, however, allows the spectral width to be narrowed and, thanks to our patented technology, the exact wavelength to be selected from the LED spectrum.</p><p>This version of the dual UV detector measures at two fixed wavelengths given by the installed LEDs.</p><p>The default wavelengths are 254 and 280 nm. Optionally, the customer can select a wavelength in the range 254-600 nm in 1 nm steps.</p><p>The unit is controlled by an innovative printed circuit board that powers the LEDs, controls the unit, and has many new features including higher sampling speed.</p><h3><strong>Key features and benefits</strong></h3><ul><li>Sharp, high, and symmetrical peaks thanks to the ECOM patented technology</li><li>Excellent linearity</li><li>Excellent price-performance ratio</li><li>Compact device dimensions</li><li>Low cell heating due to LED low power supply (0.7 W) allows use for peptide analysis or FPLC</li><li>Easy flow cell replacement</li><li>Higher sampling speed</li><li>Sophisticated diagnostic software</li><li>Diodes with wavelengths of 254 and 280 nm and a PFC cell with an optical path length of 2 mm are used as standard.</li><li>Other cells and wavelengths on request.</li></ul><h3><strong>Parameters</strong></h3><ul><li><strong>Number of channels (Signals):</strong> Two fix wavelength</li><li><strong>Typical spectral half-width&nbsp;:</strong> 4</li><li><strong>Light source:</strong> LEDs</li><li><strong>Noise level at test cell (254 nm, TC 0.75 s):</strong> ± 10x10-6 AU</li><li><strong>Drift at test cell (254 nm after 1 h):</strong> 1x10-3 AU/hr</li><li><strong>Communication:</strong> Remote digital I/O</li><li><strong>Power supply:</strong> 12-24 V DC</li><li><strong>Power input:</strong> 2 W</li><li><strong>Materials in contact with mobile phase:</strong> FEP; fused silica, stainless steel (cell)</li><li><strong>Communication interface:</strong> USB-C</li><li><strong>Optical cables:</strong> no</li><li><strong>Dimensions W x H x D:</strong> 193 x 70 x 40 mm (7.60 x 2.76 x 1.57 in)</li><li><strong>Weight:</strong> 0.66 kg (1.46 Ibs)</li></ul></div>

ECOM L3320 HPLC Autosampler

An automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design reduces sample contamination and µl pick-up mode achieves zero sample loss.

<p>Autosampler L3320 is an automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design significantly reduces sample residue contamination. µl pick-up mode achieves zero sample loss.</p><p>The autosampler is designed as a&nbsp;<strong>standalone unit</strong>.</p><p>The user-friendly unit features computer control and shows information about the actual status on the unit display. Automated self-checking simplifies the usage of the autosampler, there is no need for calibration or needle position setting.</p><p>Rheodyne valve secures the reliable operation of the device.</p><p>The unit is supported by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity&nbsp;software</strong></a>.</p><h4><strong>Parameters</strong></h4><ul><li><strong>Communication</strong>: RS232,USB</li><li><strong>Syringe volume</strong>: Standard 250μl; 100μl optional</li><li><strong>Reproducibility</strong>: Full loop injection: RSD≤0.3%, Partial loopfill injection: RSD≤0.5%, μl pickup injection: RSD≤1%</li><li><strong>Control</strong>:&nbsp;PC (Clarity SW)</li><li><strong>Loop volumes</strong>: Standard 100μl; 10μl, 20μl and 50μl optional</li><li><strong>Injection Volume</strong>: Full loop injection = loop volume, Partial loop injection = ½ of loop volume, μl pickup injection = (loop volume – 3 x needle volume)/2</li><li><strong>Sample Capacity</strong>: 2 standard 48-vial tray (1.5ml vials)</li><li><strong>Pressure limit</strong>: 6000 PSI</li><li><strong>Cross Contamination</strong>: &lt; 0.02% after programmed needle wash</li><li><strong>Weight</strong>: 17kg</li><li><strong>Dimensions W x H x D</strong>: 453 x 359 x 244 mm (17.8 x 14.1 x 9.6”)</li><li><strong>Linearity</strong>: 0.9999</li><li><strong>Power supply</strong>: 100-240VAC, 50-60Hz, 110W&nbsp;</li></ul>

Products focused on HPLC distributed by ECOM spol. s r.o. - page 1

Related content

LabRulez at analytica 2026

Oligos Made Easy - Part 8: Sepapure oliGO

News from LabRulezLCMS Library - Week 13, 2026