EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety

<div><div>Researchers led by <a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en">Eva Kudová</a> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant Staphylococcus aureus.</div></div><div>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub">Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</a></h4>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;Eur. J. Med. Chem., 2026, 308, 118716<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant Staphylococcus aureus. Structure-activity relationship analysis identified compounds 13 and 16 as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound 13 significantly downregulated S. aureus virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.<h4>Instrumentation</h4><h5>Structural characterization</h5><ul><li>AVANCE III 400 MHz NMR spectrometer (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL">LTQ Orbitrap XL HRMS</a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

Metformin is currently the first-line oral drug of choice for the treatment of type 2 diabetes. It reduces glucose production in the liver, improves tissue sensitivity to insulin, and does not cause weight gain or hypoglycemia, which sets it apart from some other antidiabetic drugs. Moreover, its long-term beneficial effects on the risk of cardiovascular complications and mortality in people with type 2 diabetes have been well established. Researchers from the Institute of Physiology CAS (IPHYS) have identified a new group of blood metabolites that could help physicians objectively monitor whether patients with type 2 diabetes are taking their metformin as prescribed.<h4>New biomarkers for metformin use</h4>A team led by Tomáš Čajka from the Laboratory of Translational Metabolism at IPHYS analyzed plasma samples from 637 patients with type 2 diabetes treated with metformin and 143 non-diabetic controls using advanced metabolomics and lipidomics. Among 614 detected metabolites, they identified five from the group of N-lactoyl-amino acids (e.g. N‑lactoyl-phenylalanine), which had previously been seen as unknown signals due to missing reference spectra in databases. The levels of these metabolites in the blood closely reflected the plasma concentrations of metformin. In patients with the highest metformin exposure, the concentrations of these metabolites were up to 7.2 times higher than in controls.<h4>A sensitive “fingerprint” of the drug’s effect</h4>N-Lactoyl-amino acids are formed in cells by combining lactate and amino acids, thus reflecting the metformin-induced changes in cellular energy metabolism. “Importantly, even though they occur in plasma only at nanomolar concentrations, they reliably distinguish patients who take metformin regularly from those who take it irregularly or not at all,” emphasizes T. Čajka.<h4>Metabolomics in practice and international collaboration</h4>The study also provides a detailed description of broader metabolic changes in type 2 diabetes, including increases in lactate, organic acids, and branched-chain amino acids, as well as extensive remodelling of the lipid profile. In addition to T. Čajka’s team, collaborators from the Institute for Clinical and Experimental Medicine in Prague and the West Coast Metabolomics Center (University of California, Davis) also participated in the study.The results demonstrate that advanced metabolomics approaches developed at IPHYS within the CarDia project can find highly practical applications both in clinical studies and in personalized monitoring of treatment in patients with diabetes.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://ascpt.onlinelibrary.wiley.com/doi/10.1002/cpt.70205">Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes</a></h4>Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Veronika Hola, Stanislava Rakusanova, Oliver Fiehn, Vojtech Skop, Ivana Lankova, Iva Miskova, Terezie Pelikanova, Martin HaluzikClin Pharmacol Ther, 119: 1057-1069<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/cpt.70205">https://doi.org/10.1002/cpt.70205</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Metformin is the most widely prescribed antidiabetic drug, yet adherence remains difficult to objectively assess. Using untargeted metabolomics and lipidomics, we analyzed plasma from 637 patients with type 2 diabetes (T2D) with confirmed metformin use and 143 nondiabetic controls, annotating 614 metabolites. Patients were stratified by plasma metformin into sub-therapeutic, therapeutic, and supra-therapeutic groups, and associations were evaluated by multiple linear regression and composite metabolite ranking. Five previously unannotated features were structurally identified as N-lactoyl-amino acids, whose levels correlated strongly with plasma metformin (ρ = 0.42–0.55, P &lt; 0.0001) and increased up to 7.2-fold in the supra-therapeutic group (&gt; 2000 ng/mL). While N-lactoyl-amino acids were consistently detected in the nanomolar range, they still displayed robust and dose-dependent associations with metformin. Broader metabolic changes in T2D included elevated lactate, organic acids, and branched-chain amino acids, together with reduced urea cycle metabolites. Lipidomics showed increases in saturated triacylglycerols and diacylglycerols and decreases in cholesteryl esters, sphingomyelins, and phospholipids. These findings establish N-lactoyl-amino acids as robust, dose-responsive plasma biomarkers of metformin exposure. Despite being up to four orders of magnitude less abundant than their amino acid precursors, they sensitively reflect mitochondrial lactate overflow and pharmacodynamic adaptation, offering objective assessment of adherence.

New blood biomarkers reveal how faithfully diabetes patients take metformin

Article

Product

Environment

Food

Health

Scientific article

Science and research

Laboratories

The Czech Academy of Sciences (CAS) was established by Act No. 283/1992 Coll. as the Czech successor of the former Czechoslovak Academy of Sciences. It is set up as a complex of 54 public research institutions.

Czech Academy of Sciences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

SIELC Alltesta Gradient Automated HPLC Analyzer

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system with for measuring small and trace concentrations of various chemicals.

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more.No difficult sample preparation is necessary, and no prior experience in chromatography or analytical chemistry is required for the operator. Measurements typically take less than 5 minutes, and the Alltesta™ comes with a set of supplies sufficient for 200 analyses.<ul><li>Small foot-print, light-weight, can be used on a small table</li><li>No difficult sample preparation</li><li>Takes only a few minutes to start operating</li><li>No chemical experience is required for instrument user</li><li>User friendly, self explanatory cloud-based software</li><li>Produces easy-to-understand results within 5-10 minutes</li></ul><h4>Alltesta Gradient Automated HPLC Analyzer includes:</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1249">Alltesta™ Power Tower</a> (provides power and cloud connectivity)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta Mini Autosampler</a> (includes <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1245">Alltesta High-Pressure Switching Valve</a>)</li><li>Two <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Ultra High Pressure Mini Syringe Pumps</a> for gradient flow</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta UV-Vis Detector</a></li></ul><h4>Examples of Alltesta™-based HPLC methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Aspirin_in_Alka_Seltzer_Extra_Strength_Effervescent_Aspirin_on_Primesep_100_Column_d33816ae83.pdf">HPLC Method for Analysis of Aspirin in Alka-Seltzer Extra Strength Effervescent Aspirin</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul>See this cloud-based example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation method <a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_example_of_the_2_Naphthalenesulfonic_acid_Allura_red_Fast_Green_FCF_1_5_Naphthalenedisulfonic_acid_Tartrazine_separation_b96e648d63.jpg" alt="SIELC: SIELC Alltesta™ Gradient Automated Analyzer - example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation.">

SIELC Alltesta Cromite HPLC Analyzer

Compact and cloud-connected, the Cromite™ HPLC System delivers precise UV/Vis analysis with innovative single-ended column technology—ideal for research, QC, and education labs.

The Cromite™ HPLC System from SIELC Technologies is a compact, high-performance liquid chromatography platform engineered for research, teaching, and routine quality control. It combines professional analytical performance with intuitive operation in a lightweight, space-saving design.Designed for academic institutions, research laboratories, and QC environments in pharmaceuticals, food science, and environmental analysis, Cromite™ delivers reliable separations without the complexity of conventional full-scale systems. Its automated single-vial injection, isocratic syringe pump, and UV/Vis detection enable accurate analysis of compounds such as caffeine, vitamins, food dyes, environmental contaminants, and active pharmaceutical ingredients.Cloud-enabled control software allows users to create methods, run analyses, review chromatograms, and share data remotely from virtually any device. This connectivity makes Cromite™ well suited for distributed teams, teaching environments, and flexible laboratory setups.A key innovation is SIELC’s proprietary Single-Ended HPLC Column technology (up to 150 mm length). This design eliminates tubing and fittings, minimizes dead volume, and enables tool-free column replacement, reducing setup time and the risk of leaks. Each system is supplied with a single-ended column of choice and all essential accessories for immediate operation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg" alt="SIELC: Alltesta Cromite HPLC analyzer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg 234w," sizes="100vw" width="1536" height="1024"><h4>Key Features</h4><ul><li>Compact and Lightweight Design: Weighing only 1.45 kg and measuring 115 × 190 × 115 mm, Cromite™ fits easily into space-limited labs and classrooms.</li><li>Automated Injection: Single-vial automated injection simplifies operation and improves reproducibility.</li><li>Isocratic Syringe Pump: Flow rates from 0.1 to 4.0 mL/min with pressures up to 4000 psi ensure effective separation of complex mixtures.</li><li>UV/Vis Detection<ul><li>Standard wavelengths: 275, 460, 520, 630 nm</li><li>Custom configuration: any four wavelengths starting from 235 nm</li><li>Low noise (&lt;0.05 mAU) and 20 Hz detection rate support reliable quantification.</li></ul></li><li>Cloud-Based Software: Wi-Fi, Ethernet, and mobile connectivity enable remote method development, data analysis, and collaboration.</li><li>Single-Ended Column Technology<ul><li>Zero dead volume design</li><li>No tubing or fittings</li><li>Tool-free replacement</li><li>Supports 4.6, 3.2, and 2.1 mm ID columns up to 150 mm length</li></ul></li></ul><blockquote>We offer a special 15% discount for schools.</blockquote><h4>Examples of Alltesta™-based methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12579/injection/22619?t=3f67e3c308cc479085c836cc682e8f1a">HPLC Method of Analysis of Ascorbic Acid</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12533/injection/22589/?t=43cad31c0e924440a18874658565c3db">HPLC Method of Analysis of Theobromine, Theophylline, Caffeine and C7</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12411/injection/22415/?t=0c93f4eb855c43aebcfd4c3b1fd55ce9">HPLC Method of Analysys of Aspirine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul><h4>Specifications</h4><ul><li>Injection: Automatic, 1 vial</li><li>Pump: Syringe, isocratic</li><li>Flow Rate: 0.1–4.0 mL/min</li><li>Maximum Pressure: 4000 psi</li><li>Noise Level: &lt;0.05 mAU</li><li>Detection Rate: 20 Hz</li><li>Wavelengths (standard): 275, 460, 520, 630 nm</li><li>Wavelengths (custom): Any four starting from 235 nm</li><li>Supported Columns: Single-Ended HPLC Columns (4.6, 3.2, 2.1 mm ID)</li><li>Column Length: ≤150 mm</li><li>Connectivity: Wi-Fi, Ethernet, Mobile network</li><li>Power Supply: 100–240 V, 50–60 Hz</li><li>Operating Temperature: 0–40 °C</li><li>Dimensions (W × H × L): 115 × 190 × 115 mm</li><li>Weight: 1.45 kg</li></ul><h4>Typical Applications</h4><ul><li>Pharmaceutical Analysis – API identification, formulation testing, and stability studies</li><li>Environmental Testing – Detection of contaminants in water and soil</li><li>Food &amp; Beverage – Analysis of vitamins, additives, and preservatives</li><li>Academic Laboratories – Hands-on chromatographic training and research</li><li>Materials &amp; Chemical Research – Evaluation of additives, catalysts, and specialty chemicals</li></ul><blockquote>For pricing information, please contact: <a>alltesta@sielc.com</a></blockquote>

SIELC Alltesta Mini HPLC Autosampler

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC.

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC. With its proprietary alpha/beta mechanical design, this compact autosampler offers direct and comprehensive control of its features, making integration quick and easy. Users can tailor the mini autosampler to their needs with customizable valve configurations, tray designs, and syringe volumes and pressures. Additionally, a built-in shaking feature enables sample mixing before injection and/or simple sample extraction within the vial.
This compact autosampler comes designed with original equipment manufacturer (OEM) customization in mind. Custom shape, color, and communication interfaces can be adapted to integrate with any system. Comprehensive software integration support is provided. Other options include metal-free sampling and reagent addition and mixing capabilities.
<ul>
<li>FRACTION COLLECTION CAPABILITY: A simple software modification can quickly convert this small autosampler into a fraction collector.
</li>
<li>REACTOR PROBE SAMPLER CAPABILITY: A simple software modification can quickly convert this small autosampler into a reactor probe sampler.
</li>
</ul>
This miniature autosampler can also be used as a component of our <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.
<blockquote>
<h4>The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested.</h4>
</blockquote>
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 3 kg / 6.6 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48 vials or 96-well plate</li>
<li>Pressure: 5000 psi</li>
<li>Needle Wash: Inside and outside</li>
<li>Syringe Capacity: 120µL high-pressure / 4000µL low-pressure stainless / 4000µL low-pressure ceramic</li>
<li>Valve options: 6×2, 7×6 and 10×9 (more details here).</li>
<li>Volume Accuracy: 0.1µL</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Mini_Autosampler_A_very_simple_schematic_operation_of_the_Alltesta_Autosampler_b79b0ad187.jpg" alt="SIELC: Alltesta™ Mini Autosampler - A very simple schematic operation of the Alltesta™ Autosampler."/>
<h4>Software</h4>
The Alltesta™ Autosampler can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb).</li>
<li>remotely through our cloud software (as a part of Alltesta™ Analyzer or Alltesta™ Mini Analyzer),</li>
</ul>
Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Mini High-Pressure Switching HPLC Valve

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications.

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications. It features flexible flow switching at varying flow rates and can be used independently or as part of the <a href="https://lcms.labrulez.com/products/124">Alltesta™ Analyzer</a> or <a href="https://lcms.labrulez.com/products/1244">Alltesta™ Autosampler</a>.
With customizable options, including up to 10 available ports on the stator, the valve can be tailored to specific needs. It is built to handle pressures up to 5000 psi (350 bar) and boasts a fast 60 degree switch time of 200 milliseconds.
<h4>Specifications</h4>
<ul>
<li>Power requirements: USB type B</li>
<li>Dimensions (WHL): 63 x 46 x 128 mm / 2.48 x 1.81 x 5.04 in</li>
<li>Weight: 0.45 kg / 0.95 lb</li>
<li>Switching speed for 60 degree: 200 ms</li>
<li>Max pressure: 350 bar (5000 psi)</li>
<li>Ports style: 10-32 thread with conical 1/16″ tube compression type seal</li>
<li>Materials in contact with liquid: coated SS316, Vespel® or PEEK or PTFE</li>
</ul>
<h4>Available Configurations</h4>
<ul>
<li>6x2 Valve</li>
<li>7x6 Valve</li>
<li>10x9 Valve</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Mini_High_Pressure_Switching_Valve_Available_Configurations_72e3b13ea2.jpg" alt="SIELC: SIELC Alltesta™ Mini High-Pressure Switching Valve - Available Configurations."/>
<h4>Software</h4>
The Alltesta™ High-Pressure Switching Valve can be controlled:
<ul>
<li>directly via serial commands (option with greatest flexibility, Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our software, allowing to use the whole functionality of the unit without the need for automation (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 6 Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_7.zip">Windows 7, 32/64 bit</a>, 127 Mb),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Analyzer</a>)</li>
<li>operator may also simply press the button on the front of the device to switch it to the next position</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
<ul>
<li>A High-flow version of the valve (preparative) is available</li>
<li>A metal-free version of the valve is also available.</li>
</ul>
<h4>Valve Rotor Position Indication</h4>
LED color indicates the valve’s rotor position. By pressing the LED, the rotor can be advanced by one position. Depending on the valve configuration, the second press will either advance the rotor forward or backward. Position 1 – LED Green Position 2 – LED Navy Position 3 – LED Purple Position 4 – LED Blue Position 5 – LED Red Position 6 – LED White
<h4>Applications</h4>
<ul>
<li>Column Switching</li>
<li>Solvent Selection</li>
<li>Sample Injection</li>
<li>Sample Cleaning</li>
</ul>

SIELC Alltesta™ Mini LHS (Liquid Handling Station)

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling.

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling. With its compact design, proprietary alpha/beta mechanical structure, and adjustable operation speeds, it offers comprehensive control for quick and seamless integration with other equipment. The station comes equipped with a built-in sample shaking feature for in-vial sample mixing and extraction, and a needle washing station capable of accommodating up to four solvents.
Designed with Original Equipment Manufacturer (OEM) customization in mind, the Alltesta™ Mini Liquid Handling Station can be tailored to fit any system’s requirements. Custom needle lengths and trays enable the device to accommodate various types of vials. An array of integrated valves and a measuring syringe can be added to the device. Additional customization options, such as metal-free flow paths and customer-specified enclosure colors, are also available.
Comprehensive software integration support is provided to ensure smooth operation and functionality.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 2.25 kg / 5 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48-vial tray or 96-well plate</li>
<li>Needle Wash: Inside and Outside</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<h4>Software</h4>
The Alltesta™ LHS can be fully controlled via serial commands. Alltesta™ protocol available upon request, please contact us at support@sielc.com. Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Mini Syringe Pump

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements.

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements. It can refill prior to each run in less than a minute, and can produce flow rates from as low 1 μL/min to as fast as 4000 μL/min with pressure up to 4000 psi (285 bar).
The Mini Syringe Pump allows for several communication options via its USB/Serial port. Just like the rest of our Alltesta™ line of devices, our serial protocol allows to control and automate every function of the device. We also provide free Windows-based software allowing full control of the device.
<h4>Specifications</h4>
<ul>
<li>Syringe volume: 5.8 mL</li>
<li>Power Requirements 24 VDC</li>
<li>Current Requirements Up to 1.5 A</li>
<li>Dimensions (WHL) 63x167x128 mm / 2.48×6.57×5.04 in</li>
<li>Weight 1.65 kg / 3.7 lb</li>
<li>Flow Rate Range 0.001 to 4 mL/min</li>
<li>Speed Accuracy 1% of full-scale</li>
<li>Linearity Full Range 0.001 to 4.0 mL/min</li>
<li>Pressure 4000 psi (285 bar) continuous</li>
<li>Pressure accuracy ±1 psi</li>
<li>Operating Temperature 0 to 40° C, non-condensing</li>
<li>Inlet/Outlet Thread 10-32 UNF compression type</li>
<li>Materials in contact with liquid SS316, PTFE, PEEK</li>
<li>Communication USB virtual serial port</li>
</ul>
<h4>Software</h4>
The Mini Syringe Pump can be controlled:
<ul>
<li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb)</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
Metal-free version of the pump includes no metal contact pump head and can be operated up to 4000 psi.

SIELC Alltesta Mini UV-Vis Detector

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it.

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it. The Mini Detector is ideal for use in HPLC, Flow Injection Analysis (FIA), and preparative and flash chromatography.
The device comes standard with four different wavelength channels, including 470 nm (Blue), 520 nm (Green), 630 nm (Red), and a choice between 255 nm or 275 nm for the UV channel. The Mini Detector features a simple setup process with a USB interface that allows it to communicate with the <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> or <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a> or integrate with other instrumentation.
Powered solely by its USB port, this mini detector is capable of monitoring multiple analytes simultaneously. It has a low optical cell volume of &lt; 3 µL, which results in minimal dispersion even at low flow rates, and can collect data at rates of up to 20 sps for all four channels, making it suitable for demanding applications. The Mini Detector boasts low noise and linearity over more than 4 orders of magnitude.
In addition to its compact size and versatility, the Mini Detector also has zero warm-up time and exceptional durability. The detector cell volume is available in two sizes: 3 μL and 10 μL.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 63 x 46 x 116 mm / 2.48 x 1.81 x 4.57 in</li>
<li>Weight: 0.25 kg / 0.55 lb</li>
<li>Sample Cell Volume: &lt; 3µL</li>
<li>Cell Optical Path: 7.0mm</li>
<li>Liquid Ports: 10-32 UNF</li>
<li>Wavelength Range: 250nm &amp; up</li>
<li>Simultaneous Wavelengths: Up to 4</li>
<li>Data Rate: 20 sps</li>
<li>Noise Level: 0.03 mAU</li>
<li>Power: USB Type B</li>
</ul>
<h4>Software</h4>
The Multi-wavelength Detector can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 127Mb)</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>).</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Power Tower for HPLC Alltesta

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free cloud software at hplc.cloud.

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free <a href="https://lcms.labrulez.com/products/1250">cloud software</a> at <a href="https://hplc.cloud/">hplc.cloud</a>. It serves as a bridge between your laboratory and a centralized data storage hub. With the Power Tower and your free HPLC.cloud subscription, you can access your equipment and experiment results from anywhere, on any device, at any time, using any interface!
<h4>Specifications</h4>
<ul>
<li>Voltage Range: 80 ~ 264VAC</li>
<li>Rated Power: 140W</li>
<li>Dimensions (WHL) - 61 x 115 x 255 mm / 2.4 x 4.53 x 10.04 in</li>
<li>Weight: 1.05 kg / 2.3 lb</li>
<li>Sampling Rate: 20 Samples per second</li>
<li>Resolution: -1 to 2.5V that corresponds to -1000 to 2500mAU</li>
<li>Noise Level: Less than 0.05mAU</li>
<li>Sync Time: Chromatogram is synced with HPLC Cloud every second</li>
<li>Operating Temperature: 0 to 40° C, non-condensing</li>
</ul>
<h4>Ports</h4>
<ul>
<li>4 USB-A</li>
<li>4 Power outputs 24V, 2A</li>
<li>1 CAT5 ethernet connector</li>
<li>1 Galvanic isolated analog input -1V to +2.5V</li>
</ul>
<h4>Key Functionality</h4>
Start &amp; stop injections; analyze chromatograms; control the detector, valve, autosampler, and pump via HPLC.cloud
<ul>
<li>Real-time chromatogram streaming</li>
<li>Connect up to 4 Alltesta™ units via 4 USB ports</li>
<li>Build and record sequences</li>
<li>Create and store methods</li>
<li>Keep your sample information and analysis results in the cloud, never lose your data</li>
<li>Build calibrations from standards, then analyze and quantitate samples</li>
<li>Start, stop, and edit your analysis remotely from anywhere in the world</li>
<li>Access your data via PC, tablet, or mobile phone</li>
<li>Search your results online</li>
</ul>

SIELC HPLC Software - HPLC.cloud

SIELC has developed the first and only HPLC software that is accessible via the cloud.

<h5>Each <a href="https://lcms.labrulez.com/products?instrumentationItems=27&amp;search=SIELC">instrument</a> is connected directly to our secure cloud server via an Ethernet line.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>All instrument parameters are visible and adjustable on the fly from any internet-enabled device. Instrument settings are stored as methods, which can be retrieved and used for specific compounds.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_2_f626a8f395.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>Multiple samples can be organized in a sequence with various methods. Results can be compared and quantitated:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_3_6eb75a29c9.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
Please see an online example of the separation results <a href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.
<h5>With your Alltesta and HPLC.cloud free subscription, you can analyze almost any compound from the palm of your hand. For example, you can analyze the dyes that are in your favorite purple sports drink:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_4_b8e9abd33a.jpg" alt="SIELC: Separation of Blue 1 and Red 40 dyes in Gatorade Zero – Grape."/>
<h5>Alternatively, you can analyze the caffeine content in your morning coffee:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_5_f39557543e.jpg" alt="SIELC: Retention of Caffeine."/>
<h5>Another option is to separate and analyze the vitamin content in your daily multi-vitamin:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_6_bbf8e002a3.jpg" alt="SIELC: Separation of Vitamin C and Vitamin B2."/>

SIELC Samplify Automated sampling system

Samplify Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate

Samplify™ Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate. It also allows to dilute the sample with assigned dilution ratio and can provide reaction quenching to preserve the sample state at time of sampling. It has two main components: an autosampler and a probe. The Samplify™ &nbsp;controlled and setup by a PC software.<h3>Description</h3>It is a common task in the lab to periodically sample a reactor or a fermenter to obtain information about reaction kinetic, completion and have a tangible representation of intermediate states of a dynamic process. This usually done manually with pipettes or syringes, using stopwatch to measure time and requires to be in the lab during the entire process.With Samplify it can be done in automated mode. When an automated sampling process is used it increases accuracy, safety, and productivity. It reduces potential samples cross contamination, and exposure of the reaction mixture to oxygen or moisture, when anaerobic or moisture free conditions are required.<ul><li>Based on a proprietary alpha/beta mechanical configuration, Alltesta Miniature Autosampler and patent pending sample probe offers affordable and reliable automation for many analytical and liquid-handling applications.&nbsp;</li><li>Small controllable volume of sample can be programmed and precisely removed from the enclosed or open system. The sample will then be transferred to a vial with inert solvent with measured volume to create a controllable dilution factor.</li><li>A quench solution can be automatically preloaded in each vial to provide termination of reaction or biological process, and preserve sample content in the time of probing.</li><li>In order to effectively mix samples with diluents or quenching reagents a shaking capability of the autosampler tray can be initiated after each sample cycle.</li><li>PC based software allows quick, but flexible, setup of the sampling experiment. Several preloaded methods can be quickly adapted to a particular needs of the user.</li><li>For temperature sensitive samples the entire autosampler can be placed in the temperature controlled environment.</li><li>The device small size allow to place it close to the reactor and use short connection lines to minimize dispersion. Also a small cooler can be used to keep samples in cold conditions after they are collected.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg" alt="SIELC: SIELC Samplify™ Automated sampling system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg 245w," sizes="100vw" width="1346" height="663"><h3>Specifications</h3><h4>Samplify™ Autosampler:</h4><ul><li>Syringe volume: 4.0 mL</li><li>Pressure: up to 250 psi</li><li>Valve configuration: 7×6</li><li>Power Requirements: 24 VDC</li><li>Current Requirements: Up to 1.0 A</li><li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li><li>Weight: &nbsp;3 kg / 6.6 lb</li><li>Inlets/Outlets: Thread 10-32 UNF compression type</li><li>Materials in contact with liquid: SS316, PTFE, PEEK</li><li>Tray capacity: 48 vials 1.8 mL volume or 96 wells plate</li><li>Communication: USB virtual serial port</li></ul><h4>Samplify™ Probe:</h4><ul><li>Connection: requires Alltesta-Samplify autosampler</li><li>Dimensions: 1 x 1 inch, DIA ¼ inch. Immersion length up to 6 inch</li><li>Materials in contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 µL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: &nbsp;200 °C</li></ul><h3>Software</h3>The Samplify can be controlled:<ul><li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li><li>remotely through our cloud software (autosampler.cloud),</li><li>directly via our Samplify PC software, allowing to fully control all the parameters of the sampling operations</li></ul><h3>Available Custom Options</h3>Metal-free version of the syringe pump includes no metal contact pump head.

Products from SIELC distributed by Bioanalytic - page 1

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