EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety

<div><div>Researchers led by <a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en">Eva Kudová</a> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant Staphylococcus aureus.</div></div><div>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub">Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</a></h4>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;Eur. J. Med. Chem., 2026, 308, 118716<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant Staphylococcus aureus. Structure-activity relationship analysis identified compounds 13 and 16 as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound 13 significantly downregulated S. aureus virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.<h4>Instrumentation</h4><h5>Structural characterization</h5><ul><li>AVANCE III 400 MHz NMR spectrometer (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL">LTQ Orbitrap XL HRMS</a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

Metformin is currently the first-line oral drug of choice for the treatment of type 2 diabetes. It reduces glucose production in the liver, improves tissue sensitivity to insulin, and does not cause weight gain or hypoglycemia, which sets it apart from some other antidiabetic drugs. Moreover, its long-term beneficial effects on the risk of cardiovascular complications and mortality in people with type 2 diabetes have been well established. Researchers from the Institute of Physiology CAS (IPHYS) have identified a new group of blood metabolites that could help physicians objectively monitor whether patients with type 2 diabetes are taking their metformin as prescribed.<h4>New biomarkers for metformin use</h4>A team led by Tomáš Čajka from the Laboratory of Translational Metabolism at IPHYS analyzed plasma samples from 637 patients with type 2 diabetes treated with metformin and 143 non-diabetic controls using advanced metabolomics and lipidomics. Among 614 detected metabolites, they identified five from the group of N-lactoyl-amino acids (e.g. N‑lactoyl-phenylalanine), which had previously been seen as unknown signals due to missing reference spectra in databases. The levels of these metabolites in the blood closely reflected the plasma concentrations of metformin. In patients with the highest metformin exposure, the concentrations of these metabolites were up to 7.2 times higher than in controls.<h4>A sensitive “fingerprint” of the drug’s effect</h4>N-Lactoyl-amino acids are formed in cells by combining lactate and amino acids, thus reflecting the metformin-induced changes in cellular energy metabolism. “Importantly, even though they occur in plasma only at nanomolar concentrations, they reliably distinguish patients who take metformin regularly from those who take it irregularly or not at all,” emphasizes T. Čajka.<h4>Metabolomics in practice and international collaboration</h4>The study also provides a detailed description of broader metabolic changes in type 2 diabetes, including increases in lactate, organic acids, and branched-chain amino acids, as well as extensive remodelling of the lipid profile. In addition to T. Čajka’s team, collaborators from the Institute for Clinical and Experimental Medicine in Prague and the West Coast Metabolomics Center (University of California, Davis) also participated in the study.The results demonstrate that advanced metabolomics approaches developed at IPHYS within the CarDia project can find highly practical applications both in clinical studies and in personalized monitoring of treatment in patients with diabetes.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://ascpt.onlinelibrary.wiley.com/doi/10.1002/cpt.70205">Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes</a></h4>Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Veronika Hola, Stanislava Rakusanova, Oliver Fiehn, Vojtech Skop, Ivana Lankova, Iva Miskova, Terezie Pelikanova, Martin HaluzikClin Pharmacol Ther, 119: 1057-1069<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/cpt.70205">https://doi.org/10.1002/cpt.70205</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Metformin is the most widely prescribed antidiabetic drug, yet adherence remains difficult to objectively assess. Using untargeted metabolomics and lipidomics, we analyzed plasma from 637 patients with type 2 diabetes (T2D) with confirmed metformin use and 143 nondiabetic controls, annotating 614 metabolites. Patients were stratified by plasma metformin into sub-therapeutic, therapeutic, and supra-therapeutic groups, and associations were evaluated by multiple linear regression and composite metabolite ranking. Five previously unannotated features were structurally identified as N-lactoyl-amino acids, whose levels correlated strongly with plasma metformin (ρ = 0.42–0.55, P &lt; 0.0001) and increased up to 7.2-fold in the supra-therapeutic group (&gt; 2000 ng/mL). While N-lactoyl-amino acids were consistently detected in the nanomolar range, they still displayed robust and dose-dependent associations with metformin. Broader metabolic changes in T2D included elevated lactate, organic acids, and branched-chain amino acids, together with reduced urea cycle metabolites. Lipidomics showed increases in saturated triacylglycerols and diacylglycerols and decreases in cholesteryl esters, sphingomyelins, and phospholipids. These findings establish N-lactoyl-amino acids as robust, dose-responsive plasma biomarkers of metformin exposure. Despite being up to four orders of magnitude less abundant than their amino acid precursors, they sensitively reflect mitochondrial lactate overflow and pharmacodynamic adaptation, offering objective assessment of adherence.

New blood biomarkers reveal how faithfully diabetes patients take metformin

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Laboratories

The Czech Academy of Sciences (CAS) was established by Act No. 283/1992 Coll. as the Czech successor of the former Czechoslovak Academy of Sciences. It is set up as a complex of 54 public research institutions.

Czech Academy of Sciences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

SIELC Alltesta Power Tower for HPLC Alltesta

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free cloud software at hplc.cloud.

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free <a href="https://lcms.labrulez.com/products/1250">cloud software</a> at <a href="https://hplc.cloud/">hplc.cloud</a>. It serves as a bridge between your laboratory and a centralized data storage hub. With the Power Tower and your free HPLC.cloud subscription, you can access your equipment and experiment results from anywhere, on any device, at any time, using any interface!
<h4>Specifications</h4>
<ul>
<li>Voltage Range: 80 ~ 264VAC</li>
<li>Rated Power: 140W</li>
<li>Dimensions (WHL) - 61 x 115 x 255 mm / 2.4 x 4.53 x 10.04 in</li>
<li>Weight: 1.05 kg / 2.3 lb</li>
<li>Sampling Rate: 20 Samples per second</li>
<li>Resolution: -1 to 2.5V that corresponds to -1000 to 2500mAU</li>
<li>Noise Level: Less than 0.05mAU</li>
<li>Sync Time: Chromatogram is synced with HPLC Cloud every second</li>
<li>Operating Temperature: 0 to 40° C, non-condensing</li>
</ul>
<h4>Ports</h4>
<ul>
<li>4 USB-A</li>
<li>4 Power outputs 24V, 2A</li>
<li>1 CAT5 ethernet connector</li>
<li>1 Galvanic isolated analog input -1V to +2.5V</li>
</ul>
<h4>Key Functionality</h4>
Start &amp; stop injections; analyze chromatograms; control the detector, valve, autosampler, and pump via HPLC.cloud
<ul>
<li>Real-time chromatogram streaming</li>
<li>Connect up to 4 Alltesta™ units via 4 USB ports</li>
<li>Build and record sequences</li>
<li>Create and store methods</li>
<li>Keep your sample information and analysis results in the cloud, never lose your data</li>
<li>Build calibrations from standards, then analyze and quantitate samples</li>
<li>Start, stop, and edit your analysis remotely from anywhere in the world</li>
<li>Access your data via PC, tablet, or mobile phone</li>
<li>Search your results online</li>
</ul>

SIELC HPLC Software - HPLC.cloud

SIELC has developed the first and only HPLC software that is accessible via the cloud.

<h5>Each <a href="https://lcms.labrulez.com/products?instrumentationItems=27&amp;search=SIELC">instrument</a> is connected directly to our secure cloud server via an Ethernet line.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>All instrument parameters are visible and adjustable on the fly from any internet-enabled device. Instrument settings are stored as methods, which can be retrieved and used for specific compounds.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_2_f626a8f395.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>Multiple samples can be organized in a sequence with various methods. Results can be compared and quantitated:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_3_6eb75a29c9.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
Please see an online example of the separation results <a href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.
<h5>With your Alltesta and HPLC.cloud free subscription, you can analyze almost any compound from the palm of your hand. For example, you can analyze the dyes that are in your favorite purple sports drink:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_4_b8e9abd33a.jpg" alt="SIELC: Separation of Blue 1 and Red 40 dyes in Gatorade Zero – Grape."/>
<h5>Alternatively, you can analyze the caffeine content in your morning coffee:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_5_f39557543e.jpg" alt="SIELC: Retention of Caffeine."/>
<h5>Another option is to separate and analyze the vitamin content in your daily multi-vitamin:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_6_bbf8e002a3.jpg" alt="SIELC: Separation of Vitamin C and Vitamin B2."/>

SIELC Samplify Automated sampling system

Samplify Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate

Samplify™ Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate. It also allows to dilute the sample with assigned dilution ratio and can provide reaction quenching to preserve the sample state at time of sampling. It has two main components: an autosampler and a probe. The Samplify™ &nbsp;controlled and setup by a PC software.<h3>Description</h3>It is a common task in the lab to periodically sample a reactor or a fermenter to obtain information about reaction kinetic, completion and have a tangible representation of intermediate states of a dynamic process. This usually done manually with pipettes or syringes, using stopwatch to measure time and requires to be in the lab during the entire process.With Samplify it can be done in automated mode. When an automated sampling process is used it increases accuracy, safety, and productivity. It reduces potential samples cross contamination, and exposure of the reaction mixture to oxygen or moisture, when anaerobic or moisture free conditions are required.<ul><li>Based on a proprietary alpha/beta mechanical configuration, Alltesta Miniature Autosampler and patent pending sample probe offers affordable and reliable automation for many analytical and liquid-handling applications.&nbsp;</li><li>Small controllable volume of sample can be programmed and precisely removed from the enclosed or open system. The sample will then be transferred to a vial with inert solvent with measured volume to create a controllable dilution factor.</li><li>A quench solution can be automatically preloaded in each vial to provide termination of reaction or biological process, and preserve sample content in the time of probing.</li><li>In order to effectively mix samples with diluents or quenching reagents a shaking capability of the autosampler tray can be initiated after each sample cycle.</li><li>PC based software allows quick, but flexible, setup of the sampling experiment. Several preloaded methods can be quickly adapted to a particular needs of the user.</li><li>For temperature sensitive samples the entire autosampler can be placed in the temperature controlled environment.</li><li>The device small size allow to place it close to the reactor and use short connection lines to minimize dispersion. Also a small cooler can be used to keep samples in cold conditions after they are collected.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg" alt="SIELC: SIELC Samplify™ Automated sampling system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg 245w," sizes="100vw" width="1346" height="663"><h3>Specifications</h3><h4>Samplify™ Autosampler:</h4><ul><li>Syringe volume: 4.0 mL</li><li>Pressure: up to 250 psi</li><li>Valve configuration: 7×6</li><li>Power Requirements: 24 VDC</li><li>Current Requirements: Up to 1.0 A</li><li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li><li>Weight: &nbsp;3 kg / 6.6 lb</li><li>Inlets/Outlets: Thread 10-32 UNF compression type</li><li>Materials in contact with liquid: SS316, PTFE, PEEK</li><li>Tray capacity: 48 vials 1.8 mL volume or 96 wells plate</li><li>Communication: USB virtual serial port</li></ul><h4>Samplify™ Probe:</h4><ul><li>Connection: requires Alltesta-Samplify autosampler</li><li>Dimensions: 1 x 1 inch, DIA ¼ inch. Immersion length up to 6 inch</li><li>Materials in contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 µL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: &nbsp;200 °C</li></ul><h3>Software</h3>The Samplify can be controlled:<ul><li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li><li>remotely through our cloud software (autosampler.cloud),</li><li>directly via our Samplify PC software, allowing to fully control all the parameters of the sampling operations</li></ul><h3>Available Custom Options</h3>Metal-free version of the syringe pump includes no metal contact pump head.

PBI Barocycler 2320EXT Sample Preparation System

The Barocycler 2320EXT is a compact, bench top instrument that can process up to 16 samples simultaneously using PCT MicroTubes.

<h4>For the PCT-HD Sample Preparation System</h4>Pressure Cycling Technology (PCT) is a unique, patented technology platform based on repeated cycles of hydrostatic pressure between ambient (14.7 psi) and ultra-high levels. These rapid changes in pressure are used to control biomolecular interactions for applications such as accelerated proteolytic digestion, and improved extraction of cellular components, including proteins and lipids. The PCT platform allows for a high degree of safety, speed, reproducibility, and convenience in modern sample preparation protocols.The PCT-HD Sample Preparation System is a proven PCT-based workflow for processing small tissue samples for proteomic and other applications. PCT-HD combines efficient, hands-off tissue homogenization and protein extraction with pressure-enhanced digestion, for rapid, efficient and reproducible generation of peptides for MS analysis. This unique workflow combines two of PBI’s innovative sample preparation tools: the Barocycler 2320EXT and our patentpending PCT MicroPestles. PCT-HD and the Barocycler 2320EXT can provide unprecedented speed and reproducibility for biomarker discovery, yielding peptides ready for clean-up and MS within 2-4 hours from the start of tissue processing.The Barocycler 2320EXT is a compact, bench top instrument that can process up to 16 samples simultaneously using PCT MicroTubes. Features that come standard with the 2320EXT include: data input and output options to accommodate validation and quality control; computer-operated control with touch screen programming and automatic data logging; and the ability to control multiple pressure cycling parameters, such as the rate of pressure increase/decrease, both the high and low pressure levels, and the shape of the pressure profile (e.g., sine wave, square wave, and others). In addition, the 2320EXT is available with a choice of two different methods for temperature control (an external circulating waterbath for heating and cooling, or a built-in electric heater).<blockquote>Patent Pending</blockquote><h4>The Barocycler 2320EXT</h4><ul><li>Computer-operated control with Touch Screen Interface</li><li>Pressure Range: ambient - 45,000psi (310 MPa)</li><li>Choice of temperature control - heating/cooling or heating only</li><li>Max Temperature: 95˚C</li><li>Min Temperature: 4˚C with circulating waterbath, ambient with electric heating</li><li>Insulated closure for easier use at high temperatures</li><li>Chamber capacity: up to 16 samples in MicroTubes</li><li>Easily accessible USB port on front panel</li><li>Requires 110psi input air pressure</li><li>Input air pressure can be provided by an air compressor, house air or nitrogen lines, air or nitrogen tanks.</li><li>Pressure medium: Distilled Water</li></ul><h4>Touch Screen Interface</h4>Pressure Profiles are programmed via an onboard touch screen, which can be connected to a network. The instrument is also equipped with a USB port that can be used to connect a mouse or keyboard to the 2320EXT.<ul><li>Pressure Mode Settings: Barocycler, Ramp, Waveform</li><li>Cycled or Static Pressure</li><li>Network Enabled</li><li>Automatic Logging of All Run Parameters</li><li>Data Output&nbsp;</li><li>Save and Record Programs</li><li>Real-time pressure display graph shows pressure and temperature throughout the run</li><li>Touch Screen Temperature Control (with electric heating option only)</li></ul><h4>MicroTubes and MicroPestles</h4>The 2320EXT comes with a full MicroTube Adapter Kit, that includes the MicroTube Cartridges, Capper Tool, and other accessories that are required when using PCT MicroTubes in the 2320EXT. PCT μPestles, used in conjunction with PCT MicroTubes in the Barocycler, are designed for rapid, hands-off homogenization and extraction when working with minute amounts of solid tissue. PCT MicroTubes and MicroPestles are an integral part of the PCT-HD workflow.<ul><li>Process up to 3mg of solid tissue in 30uL of extraction reagent</li><li>Process as few as 5 x 104 cells</li><li>MicroTubes are constructed of non-wetting, low binding FEP plastic</li><li>MicroPestles are made from inert PTFE (Teflon)</li></ul>

Trajan Mitra Microsampling Devices - Specimen bag

The Specimen Bag Mitra format is perfect for those looking to create their own collection kits for remote sampling or maintain bulk devices for centralized sampling in clinical or field settings.

<h3>About</h3>The Specimen Bag Mitra format is perfect for those looking to create their own collection kits for remote sampling or maintain bulk devices for centralized sampling in clinical or field settings.Each Specimen Bag is sealed and includes a barcoded Mitra device with a unique identifier and desiccant. After collection, the device is placed back into the Specimen Bag to facilitate quick drying and protect the sample from external contamination.If you need logistics help, bioanalytical lab services, or kitting support, we can connect you with experienced organizations that specialize in microsampling.<h4>Build Your Own Kits or Sample Large Cohorts Remotely</h4>With this format you can collect many samples in the field and ship them back to the lab in bulk. You may prefer to insert them into your own all-in-one kit.<ul><li>10, 20, &amp; 30 µL volumes</li><li>Protective outer casing</li><li>Barcoding to manage chain of custody</li><li>Packed in specimen bag with desiccant for transport &amp; storage</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17218576460536_2"><ul><li>Indication for Use: A single-use, non-sterile device used as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses.&nbsp;</li><li>Sampler Tip Volume: 10, 20, or 30uL&nbsp;&nbsp;</li><li># of Samplers per Device: 2 or 4&nbsp;</li><li>Shelf Life: 18 months&nbsp;</li><li># Device per pack: 30/Pk</li><li>Pack content: Mitra Device, Specimen Bag with Desiccant, Sample ID Barcode, 1x IFU</li><li>Regulatory status: Mitra® devices are CE-IVD (IVDR) devices intended as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses. They are available as registered IVD Devices in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, Mitra devices are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices for the particular diagnostic testing intended.</li></ul><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Mitra%20Device%20Specimen%20Bag/VLBL-0125-Rrev00.pdf">Instructions for Use (RUO)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Mitra%20Device%20Specimen%20Bag/VLBL-0125rev00.pdf">Instructions for Use (MD)</a></li></ul><h3>Resources</h3><ul style="list-style-type:disc;"><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_2022%20Mitra%20Collateral/Mitra%20Specification%20Sheet%20(3).pdf">Mitra Device Specification Sheet</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/User%20Guides/Mitra%20User%20Guide%20Extracting%20&amp;%20Processing%20Microsamples.pdf">User Guide | How to Extract &amp; Process Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/GenTegraRNA-NEO%20for%20Mitra%20devices%20USER%20GUIDE%2011.15.23.pdf">User Guide | How to Stabilizer RNA on Mitra Microsamples with GenTegraRNA-NEO™</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions?hsLang=en">Frequently Asked Questions</a></li></ul><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3></div>Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases</li></ul>

Trajan Mitra Microsampling Devices - Microsample collection kit

The Microsample Collection Kit Mitra format streamlines the blood microsampling process, enabling both assisted and self-collection at home or in the field.

<h3>About</h3>The Microsample Collection Kit Mitra format streamlines the blood microsampling process, enabling both assisted and self-collection at home or in the field. Each kit is equipped with device, barcoding for secure chain of custody, easy-to-follow instructions, all necessary finger-prick sampling supplies, and a return-mailing envelope, all packaged for global distribution. Ideal for remote blood collection, these kits ensure that samples can be directly sent to accredited labs for precise analysis.For customization to meet the demands of large-scale projects or to optimize your workflows and logistics, please consult with our <a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/contact">Microsampling Consultants</a>. They will provide tailored recommendations to align with your project’s specific objectives.<h4>All-in-One Solution for Remote Specimen Collection</h4>Our Mitra devices can be packed in convenient kits with sterile components for safe sample collection. Kits can be shipped to your end-users.<ul><li>10, 20, &amp; 30 µL volumes</li><li>Protective outer casing</li><li>Barcoding to manage chain of custody</li><li>Packed in specimen bag with desiccant for transport &amp; storage</li><li>Contains devices &amp; supplies</li><li>Includes instructions for use &amp; return shipping envelope</li><li>Sized for easy global mailing</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_1721774838869_2"><ul><li>Indication for Use: The Microsample Collection Kit is a single-use non-sterile procedure pack intended for the collection, storage and transport of capillary whole blood specimens of specified volume.</li><li>Sampler Tip Volume: 10, 20, or 30uL</li><li># of Samplers per Device: 2 or 4</li><li># Device per kit: 1/Pk</li><li>Pack content: 1x Mitra Device, 1x Specimen Bag with Desiccant, Sample ID Barcode, Lancet (x2 or x4), Gauze (x1 or x2), Bandage (x2 or x4), 1x Plastic Shipping Envelope, 1x IFU</li><li>Shelf Life: 18 months</li><li>Regulatory status: The Mitra Blood Sample Collection Kit is a procedure pack. The kits are available as registered Medical Device in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, procedure packs are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices and Mitra Microsample collection kit for the particular diagnostic testing intended.</li></ul></div><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Collection%20Kits/VLBL-0124-Rrev00.pdf">Instructions for Use (RUO)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Collection%20Kits/VLBL-0124rev00.pdf">Instructions for Use (MD)</a></li></ul><h3>Resources</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_2022%20Mitra%20Collateral/Mitra%20Specification%20Sheet%20(3).pdf">Mitra Device Specification Sheet</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/Guidelines%20for%20Collecting%20Samples%20at%20Home-1.pdf">Guidelines for Collecting Samples at Home</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions">Frequently Asked Questions</a></li></ul><div class="tabs__tab txt--light tabs__tab--active" id="tab_1721774838869_4"><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3>Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases</li></ul></div>

Trajan Mitra Microsampling Devices - 96-autorack

The 96-Autorack™ format is designed for laboratories to efficiently batch and process Mitra microsamples using a standard 96-well format.

<h3>About</h3>The 96-Autorack™ format is designed for laboratories to efficiently batch and process Mitra microsamples using a standard 96-well format. Compatible with most 1mL &amp; 2mL collection plates and 96-channel pipettors &amp; liquid handling workstations, the 96-Autorack enhances lab productivity and integration. We provide two options: an empty 96-Autorack, perfect for accessioning microsamples from Mitra devices received from the field for building plates, extractions, and long-term storage; and prefilled 96-Autoracks with 96 Mitra samplers, ideal for method development and handling calibrators and QCs.<h4>Accession &amp; Process Multiple Mitra Samples at Once</h4>This high-throughput solution allows you to rapidly process Mitra microsamples for method development, plate building, extractions - or all of the above!<ul><li>Racks available empty or filled with 96 microsamplers (10, 20, &amp; 30 µL volumes)</li><li>Filled racks come with a 2mL deep well storage plate</li><li>Compatible with 96-channel pipettors &amp; liquid-handling workstations</li><li>Options with &amp; without barcoding</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_2"><ul><li>Indication for Use: A single-use, non-sterile device used as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses.&nbsp;</li><li>Sampler Tip Volume: 10, 20, or 30uL&nbsp;&nbsp;</li><li># of Samplers per Device: 0 or 96&nbsp;</li><li># Device per pack: 1/Pk</li><li>Pack content: 1x 96-Autorack (empty or pre-filled), Sample ID Barcode, 1x Collection Plate, 1x IFU</li><li>Shelf Life: 18 months&nbsp;</li><li>Regulatory Status: Mitra® devices are CE-IVD (IVDR) devices intended as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses. They are available as registered IVD Devices in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, Mitra devices are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices for the particular diagnostic testing intended.</li></ul><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Mitra%20IFUs/2024/VLBL-0098rev04.pdf">96-Autorack Instructions For Use (IVD)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/96%20autorack/VLBL-0098-Rrev01.pdf">96-Autorack Instructions For Use (RUO)</a></li></ul><h3>Resources</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/User%20Guides/Mitra%20User%20Guide%20Extracting%20&amp;%20Processing%20Microsamples.pdf">User Guide | How to Extract &amp; Process Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/GenTegraRNA-NEO%20for%20Mitra%20devices%20USER%20GUIDE%2011.15.23.pdf">User Guide | How to Stabilizer RNA on Mitra Microsamples with GenTegraRNA-NEO™</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions?hsLang=en">Frequently Asked Questions</a></li></ul><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_3"><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_4">Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases&nbsp;</li></ul></div></div></div>

Quantitative analysis of proteins (Bioanalytic) 

In our laboratory, proteomic analyses, including quantitative analysis of proteins, are performed by HPLC-MS or HPLC-MS/MS methods on a ZenoTOF 7600 high-resolution mass spectrometer (SCIEX).

Quantitative analysis of proteins is important in many industries and applications. Proteins include a diverse group of compounds that participate in virtually every biological process. Proteins are building blocks, hormones, transport molecules, cell receptors, cell growth and differentiation factors. Because of this, the analysis of protein interactions is crucial in drug design and production.Proteins are macromolecules - the basis of their structure are polypeptide chains containing from 100 to more than 10000 amino acids (10 - 100 kDa). Proteins are found in simple and complex forms. Complex proteins contain a non-protein component (sugar, lipids, metal ions or dyes) in their structure.In our laboratory, proteomic analyses, including quantitative analysis of proteins, are performed by HPLC-MS or HPLC-MS/MS methods on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=ZenoTOF+7600">ZenoTOF 7600</a> high-resolution mass spectrometer (SCIEX). We process the obtained results using Sciex OS, Spectronaut, Skyline software.<h4>MS vs MS/MS</h4>Quantitative analysis of intact protein (whole protein in intact form) leads to the concentration (determined from a prepared curve from a standard (with or without matrix) and confirmation of the identification by superimposing (on a mirrored basis) the MS spectrum of the protein determined in the sample to the MS spectrum of the standard. For your needs, we can generate a list of protein-structured compounds (impurities) that were identified in the sample and determine the ratio of their signal area to the signal area of the analyzed protein.The result of proteomic quantitative peptide analysis allows precise and much more sensitive determination of protein concentration. The analysis is based on MS/MS data obtained for peptides obtained by digestion with proteolytic enzymes. The reported result consists of the concentration of the protein under study and confirmation of its presence based on the determined amino acid sequence. For such analyses, it is recommended to add standards internal standards.<blockquote>Our offer:<ul><li>For proteins and peptides with low masses - intact analysis with MS detection;</li><li>Proteins with masses &gt;20kDa require optimization of the digestion method and MS/MS detection. This results in specific signals from the analyzed protein in the mixture/matrix;</li><li>From complex matrices (including serum, cell culture, fungal suspension), sample analysis involves several steps:&nbsp;<ul><li>Sample purification;</li><li>Digestion to obtain unique peptides to distinguish the analyzed proteins from other proteins in the sample;</li><li>Sample preparation requires appropriate method optimization and validation (let's set validation criteria individually);</li></ul></li><li>Reported quantitative result includes confirmation of the presence of the protein in native form or a peptide derived from it (usually confirmation includes at least two peptide sequences);</li><li>Statistical analysis using tools: MarkerView (PCA), GraphPad Prism, Trends, Sciex OS.</li></ul>To optimize and validate the method, it is necessary to provide a standard, in the case of proteins with high masses - it is recommended to add an internal standard.See also:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/service/identyfikacja-bialek/">Protein identification</a>,</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1296">Analysis and characterization of monoclonal antibodies</a>.</li></ul></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_analysis_of_proteins_1_de494646f2.jpg" alt="Bioanalytic: Quantitative analysis of proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_analysis_of_proteins_1_de494646f2.jpg 200w," sizes="100vw" width="777" height="606">

Identification of the substance – what is in your sample? (Bioanalytic)

We are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.

<h4>Substance Identification - Looking for answers to what's in your sample? - we can help you.</h4>Substance identification based on LC-MS/MS techniques can involve single compounds or mixtures of substances. In our analyses, we rely primarily on the knowledge and skills we have acquired after many years of work. For analysis, we use full laboratory facilities for sample preparation and high-resolution mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/o-nas/">equipment</a>).We use different work algorithms depending on the material we are dealing with and the analytical task to be solved. We use DDA (IDA) or DIA (SWATH) methods or targeted approaches (e.g. MRMhr) if we know what to expect. Thus, we are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg 245w," sizes="100vw" width="1881" height="1156"><h4>COST OF SERVICE</h4>We determine the cost of the service individually. The final cost is influenced by many factors related to the nature of the sample itself and the client's expectations. Thus, the price depends, among other things, on the complexity of the sample preparation procedure, the scope and number of analyses, etc.The service includes (depending on individual arrangements):<h5>Development of sample preparation procedure</h5><ul><li>Depending on the matrix and test substances.</li></ul><h5>Development of analytical methods</h5><ul><li>Application of high-resolution LC-MS/MS analyses using DDA (IDA) or DIA (SWATH) experiments and software to support the development of targeted screening methods.</li></ul><h5>Analysis of results includes:</h5><ul><li>finding and identifying the substances of interest, interpreting MS/MS spectra and proposing a structural formula.</li><li>Comparative analysis of sample series, e.g. PCA,</li><li>profiling of analytes in sample series</li></ul><h5>Reporting of results</h5><ul><li>Compile results into easy-to-read reports.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg 245w," sizes="100vw" width="1919" height="1208">

Characteristics of monoclonal antibodies (Bioanalytic)

At LabExperts, proteomic analyses are performed on a ZenoTOF 7600 high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation.

Antibodies are molecules made up of 4 polypeptide chains: two heavy (H - high-chain) linked to each other by a specific type of chemical bond - a disulfide bridge, and two light (L - light-chain) linked by an identical bond to the heavy chains. Monoclonal antibodies (mABs) are used in various forms - as free molecules, in the form of a drug-antibody combination, and in the form of an immunoconjugate, a drug-antibody-biopolymer combination.Their main use is in highly specific targeted therapy for cancer, cardiovascular, autoimmune and nervous system diseases. They are also used in the treatment of osteoporosis, Alzheimer's disease or transplantation. The range of applications of monoclonal antibodies is systematically expanded. The introduction of a new antibody to the market requires complex product characterization, which involves numerous proteomic analyses, which are performed by LC-MS/MS techniques, among others.<h4>LC-MS/MS</h4>The characterization of monoclonal antibodies involves many aspects, including, but not limited to, a suite of analyses related to confirmation of the structure, chain sequence and degree of modification of the antibody. Post-translational modifications (PTMs) are chemical changes that occur after the translation process. These changes generally result in an increase in the immunogenicity of the drug. For this reason, it is essential to monitor modifications as critical quality attributes (CQAs) during both the production and storage of the drug. To ensure monitoring of CQA points, the primary structure of the protein is characterized based on peptide mapping (peptide mapping) analysis. The protein under study is subjected to proteolytic digestion using enzymes such as trypsin, chymotrypsin, Lys-C, Glu-C. As a result of various proteolytic enzymes, a wide range of peptides is obtained. Ultimately, coverage of the entire sequence of the protein under study is obtained.<figure class="image"><img style="aspect-ratio:1536/947;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg" alt="Bioanalytic: Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg 245w," sizes="100vw" width="1536" height="947"></figure>At LabExperts, proteomic analyses are performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=ZenoTOF+7600">ZenoTOF 7600</a> high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation. This provides us with data for unambiguous identification of protein-building amino acids. We process the results using Sciex OS, Skyline and Spectronaut software.<blockquote>We perform:<ul><li>Whole protein analyses - Intact - to determine the exact mass and confirm the theoretical mass;</li><li>N-glycosylation profile determination (based on MS spectrum reconstruction):<ul><li>Glycosylated form;</li><li>Deglycosylated;</li><li>Glycosylated with reduced disulfide bridges;</li><li>Deglycosylated with reduced disulfide bridges.</li></ul></li><li>Peptide maps:<ul><li>Protein digestion with various proteases so that sequence coverage is close to 100%<ul><li>Analysis of PTMs:</li><li>Location of modifications</li></ul></li><li>[%] of modifications compared to the unmodified sequence</li></ul></li><li>TiPOS testing - (technical proof of similarity) - technical confirmation of the similarity of the protein synthesized by the customer (from several LOTs) with the target protein (provided as a template).</li></ul>In order to maintain the highest possible analytical standards, we perform monoclonal antibody analyses under the regime of an internally developed and implemented laboratory quality system based on GLP principles.See also our other proteomics services: <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/service/identyfikacja-bialek/">Protein identification</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1294">Protein quantitative analysis</a></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg 245w," sizes="100vw" width="1536" height="908"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg 245w," sizes="100vw" width="1536" height="858">

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