<div>A new study reveals that over the past decade, urinary levels of Bisphenol A (BPA) in the Czech population have dropped significantly, while its substitute Bisphenol S (BPS) has increased. Despite this shift, BPA remains the dominant bisphenol, with concentrations exceeding safe limits — confirming that everyday exposure still poses health risks. The study was led by our research group at RECETOX, in collaboration with partners from the National Institute of Public Health (NIPH Prague).&nbsp;&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_MUNI_BPA_free_is_not_risk_free_Czech_study_shows_BPA_declines_but_BPS_is_partly_replacing_it_cb7567a37a.jpg" alt="RECETOX MUNI: “BPA-free” is not risk-free: Czech study shows BPA declines, but BPS is partly replacing it: Temporal patterns of bisphenol exposure in the Czech population within the framework of regulatory measures." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_MUNI_BPA_free_is_not_risk_free_Czech_study_shows_BPA_declines_but_BPS_is_partly_replacing_it_cb7567a37a.jpg 245w," sizes="100vw" width="572" height="339">“We found that over roughly a decade (2011–2012 vs. 2019–2020), urinary levels of Bisphenol A (BPA) in the Czech population dropped substantially — by 28.6 % in adults and 47.5 % in children — while levels of its substitute Bisphenol S (BPS) increased (121.1 % in adults, 90.0 % in children). We suggest this simultaneous decline in BPA and rise in BPS likely reflect manufacturers’ shift to BPA-free alternatives in response to regulation,” explains the main author of the study Daria Sapunova.&nbsp;Despite legislative measures, BPA still remains the dominant bisphenol in the Czech population, occurring at the highest concentrations and at the levels significantly higher than its substitutes BPS and Bisphenol F (BPF). Moreover, BPA levels in all age groups remain higher than what health experts consider safe, suggesting that everyday sources of exposure are still present around us and continue to pose risks.&nbsp;&nbsp;“Our results highlight the need for policymakers to regulate bisphenols as a group and prevent harmful substitutions like the shift from BPA to BPS and BPF, supported by continuous human biomonitoring. BPA is a well-known endocrine-disrupting chemical widely used in plastics and food packaging, with potential risks to human health. Endocrine-disrupting chemicals can interfere with our hormonal system and disturb its proper functioning,” adds Daria Sapunova.&nbsp;Studies link BPA exposure to altered puberty timing, reduced fertility changes in sperm quality. Additionally, BPA is associated with a higher risk of obesity and type 2 diabetes, likely due to its effects on metabolic regulation. Children and pregnant women are considered particularly vulnerable, and exposure during embryonic development can lead to irreversible changes later in life. Some studies also show associations between BPA exposure and changes in brain development, behavior, attention, and stress responses in children.&nbsp;&nbsp;“Despite bans and restrictions of BPA, our findings show that people — particularly children — remain exposed. The rise in BPS levels is worrisome because BPS was originally introduced as a safer alternative, but toxicological studies suggests it can poses similar health risks. Although the Czech population is still exposed to these harmful chemicals, legislative measures targeting BPA are effective and we should continue in this direction. In the case of children, we managed to reduce BPA concentrations by about half in a decade,” says Daria Sapunova.&nbsp; The researchers analyzed first-morning urine samples from over 1,000 individuals sampled in two separate Czech cohorts: DEMOCOPHES-CZ mother–child pairs (2011–2012), and CELSPAC (2019–2020) covering school children, teenagers and young adults. Using accredited laboratory methods, they quantified total urinary concentrations of BPA, BPS, and BPF. They then examined how bisphenol levels differed over time and whether they were influenced by factors such as age, gender, smoking habits, or the season during which samples were collected. To evaluate potential health risks, the researchers compared all measured BPA concentrations with established health-based guidance values to determine whether different age groups exceeded safe exposure levels. &nbsp; Authors thank all collaborating field workers, laboratory and administrative personnel, and especially the cohort participants who invested their time and provided samples and information for this study. The team thanks also the Research Infrastructure RECETOX RI (No. LM2023069) for a supportive background. The study was supported from the European Union's Horizon 2020 research No. 857560 (CETOCOEN Excellence) and from the Horizon Europe programme under grant agreement No. 101057014 (PARC). Also supported by the European Commission through the LIFE+ programme under grant agreement LIFE09 ENV/BE/000410. There are no particular limitations on communication arising from the funding.&nbsp;<blockquote>Learn more: <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1186/s12302-025-01244-0">https://doi.org/10.1186/s12302-025-01244-0&nbsp;</a></blockquote></div>

“BPA-free” is not risk-free: Czech study shows BPA declines, but BPS is partly replacing it

When performing analyte extraction with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50">SPE</a> we expect 100% recovery. If recovery is lower, this would suggest there is an issue with retrieval of the analyte from the sorbent. But what are some of the reasons you may see a recovery greater than what you were expecting (or over 100%)?<h3>Common problems</h3><ul><li>Co-eluting interferences</li><li>Contaminants from the solvent or sorbents selected</li><li>Internal standard is compromised making your reference point inaccurate</li><li>Recovery calculations are inaccurate</li></ul>Let's examine each of these common problems.<h4>Co-eluting interferences</h4>These are typically present in the matrix and the goal of the SPE step is to remove them. If you are experiencing recoveries greater than 100%, the first step would be to check the eluent using an orthogonal analytical technique to identify that the increased recovery is a result of a co-elution.&nbsp;Where a co-elution is identified, there are a few possible solutions.<ol><li>Modify the wash steps of the SPE method to selectively remove the impurity during washing</li><li>Select an alternative SPE sorbent which will better remove these impurities. This is easier if the analyte / impurity can be selectively ionized, as an ion-exchange sorbent will very often afford cleaner extracts than either reversed or normal phase solutions</li><li>Adjust your analytical method to resolve the 2 peaks analytically, negating the necessity to remove this impurity during the SPE step.</li><li>Run a blank plasma sample; this will allow you to ascertain the impurities present and use this blank in the calculations to subtract from your recovery</li></ol><h4>Contaminants from the solvent or sorbent</h4>These will typically be impurities present in the starting materials which are similar in nature to your compound of interest, and as a result co-elute with it during SPE and subsequent analytical analysis. You can follow the steps above to remove co-eluting impurities, or alternatively as this is not an impurity present in your matrix, you can identify the level present in the sorbent using blank runs and subtract this from your main peak during the calculation. Prior to addition of your sample if you equilibrate your sorbent with the elution solvent this should flush out potential contaminants from the sorbent. For impurities introduced from the solvent, typically switching the solvent should resolve this.<h4>Internal standard is compromised</h4>If your internal standard is not showing 100% recovery, your subsequent calculations will be inaccurate. It is always good practice to use an internal standard similar in nature to your compound of interest as this will give the more consistent reference point for your method.&nbsp;If there is concern your internal standard is not showing good recovery from the SPE material using your method, you can use an external standard which is added to the elution solvent to calculate absolute recovery.<h4>Recovery calculations are inaccurate</h4>It is important to follow this step wise to ascertain where the errors are appearing.<ol><li>Calculation Errors - Check all calculations and account for all dilution factors</li><li>Integration Errors - Reintegrate all peaks and use manual calculations to confirm</li><li>Analytical method errors – Ensure the analytical method used allows correct integration and calculation. Ensure no strong solvents are used for injection and run a calibration across a number of dilutions to ensure linearity. Troubleshoot your analytical method to determine any factors which may lead to poor reproducibility</li><li>Ensure detections selected are appropriate and working correctly.</li></ol>We hope this tip was useful! Check out our resources on <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/solid-phase-extraction">solid phase extraction</a>. See you next month for another tip!

Why are my recoveries greater than 100%?

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 19th January 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies, Shimadzu, Waters Corporation and poster by Thermo Fisher Scientific!<h3>1. Agilent Technologies: Automated Bioprocess Monitoring with Agilent 1290 Infinity III Bio Online LC Solutions</h3>Process analytics of L-alanine production using genetically modified Vibrio natriegens<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_automated_bioprocess_monitoring_1290_infinity_bioonline_solutions_5994_8865en_agilent_9f33de6ddf.pdf">Full PDF for download</a></li></ul>Bioprocess engineering is a challenging field because of the constantly changing nature of bioprocess models which make them difficult to validate.1 Consequently, process control needs to be developed at various levels using strategies tailored to these specific requirements.1 The performance and effectiveness of the bioreactor are determined by key performance indicators such as product yield, titer, production rate, and quality of the product.1 These factors can be quickly influenced by changes in process variables and the raw materials used.1,2 The ability to react quickly to problems is therefore crucial for preserving ongoing production, a factor that can be particularly significant in bioprocesses due to the substantial cost and time investmented.1,2 Such challenges can be tackled by constantly monitoring the bioprocess, which requires the ability to analyze and collect data quickly. Therefore, monitoring is of the utmost importance.&nbsp;This application note describes the advantages of using an Agilent 1290 Infinity III Bio Online LC solution with Agilent Online LC Monitoring software. A bioreactor was interfaced with a Flownamics Seg-Flow S3 sampling system for aseptic withdrawal and an Agilent 1290 Infinity III Bio Online LC to make an hourly analysis.&nbsp;This is a fully automated approach for taking sterile samples from a bioreactor and preparing or diluting them. In addition, the 1290 Infinity III Bio Online LC solution displays data in near real time, which is a crucial element for effective bioprocess&nbsp;monitoring.<h4>Experimental&nbsp;</h4><h5>Equipment&nbsp;</h5>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III+Bio">1290 Infinity III Bio </a>Online LC comprised the following&nbsp;modules:&nbsp;<ul><li>Agilent 1290 Infinity III Bio Flexible Pump (G7131A) with an InfinityLab Quick Change Bio inline filter assembly (part&nbsp;number 5067-1607), 2.1 mm id, 0.2 µm pore size&nbsp;</li><li>Agilent 1290 Infinity III Bio Online Sample Manager (G3167B) with Sample Thermostat (option #101) clustered with Agilent 1290 Infinity Valve Drive (G1170A), featuring a reactor valve pod (part number 5067-6680) and Online LC Monitoring Software&nbsp;</li><li>Agilent 1290 Infinity III Multicolumn Thermostat (G7116B) equipped with an Agilent Quick Connect Bio Heat Exchanger Std. (G7116-60071)&nbsp;</li><li>Agilent 1290 Infinity III Diode Array Detector (DAD) (G7117B), equipped with a Bio Max-Light Cartridge Cell (G4212-6008), 10 mm, 1 µL&nbsp;</li><li>Agilent 1260 Infinity High-Performance Degasser (G4225A) is connected between S1 (transport solvent) solvent line and Agilent 1290 Infinity III Bio Online Sample Manager (G3167B)&nbsp;</li></ul><h5>Software&nbsp;</h5><ul><li>Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=OpenLab+CDS">OpenLab CDS</a> version 2.8 or later versions&nbsp;</li><li>Agilent Online LC Monitoring Software version 1.3 or later&nbsp;versions&nbsp;</li><li>Flownamics FlowWeb control&nbsp;software</li></ul><h5>Instrumental configuration&nbsp;</h5>The 1290 Infinity III Bio Online LC solution was used with a Seg-Flow interface for automatic sampling and online analysis (see Figure 1). The Seg-Flow interface was used to withdraw samples from a bioreactor through a Flownamics F-Series 310 FISP probe with a ceramic membrane filter (0.2&nbsp;µm pore size) from a 5 L bioreactor. The drawn samples were sent through the built-in sample collection cup on the Seg‑Flow to the LC and the samples were retained into vials. The collected samples were then automatically derivatized by using an injector program and subsequently analyzed. A more detailed description of the instrumental configuration has been published.3<h4>Conclusion&nbsp;</h4>For online bioprocess monitoring, a fully automated setup consisting of a bioreactor, a Flownamics sampling system, and an Agilent 1290 Infinity III Bio Online LC solution was successfully established. This setup is capable of taking aseptic samples from a bioreactor, preparing, analyzing, and processing samples without the need for manual interaction. In addition, the added value of the Agilent Online LC Monitoring software was evident in the display method and the ability to track it quickly.<h3>2. Shimadzu: Analysis of Oligonucleotide Impurities Using Single Quadrupole Mass Spectrometer</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00974_en_c50bb67c7a.pdf">Full PDF for download</a></li></ul><h4>User Benefits</h4><ul><li>Combination use of LCMS-2050 single quadrupole mass spectrometer and LabSolutions Insight Biologics provides comprehensive characterization of oligonucleotides and related impurities.</li><li>Purity calculations for target components can be performed even on poorly separated peaks by utilizing MS spectra simultaneously acquired with UV chromatogram.</li></ul>In recent years, oligonucleotide therapeutics have been paid attention as a novel drug discovery modality because of their rapid progress of development. During the synthesis of oligonucleotide therapeutics, impurities that have differing base chain lengths are generated along with the target oligonucleotide. To ensure the safety and efficacy of the drug, comprehensive detection and identification of these impurities are required.&nbsp;Reversed phase ion-pair chromatography is often employed as a method to separate the target compound from co-existing impurities. However, it is often difficult to establish analytical conditions that provide compete separation of all impurities that are formed in synthesis process due to their similar structures and properties to the principal compound of active ingredient. Against this drawback, liquid chromatography-mass spectrometry (LC-MS) is commonly used for impurity detection and identification. For analysis of oligonucleotide impurities, quadrupole time-of-flight mass spectrometers are mainly used because of their high mass resolution and ability to perform sequence and structure estimations via MS/MS analysis. However, in quality control process, the use of a single quadrupole mass spectrometer is expected to be advantageous because of providing mass information with easy operation when impurity information is already known.&nbsp;This article presents a simulated impurity analysis of synthetic oligonucleotide using the LCMS-2050 high-performance liquid chromatography-mass spectrometer and LabSolutions Insight Biologics.<h4>Analytical conditions</h4>The employed instrument setup was <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+XS">Nexera XS inert</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-2050">LCMS-2050</a>. HPLC conditions are shown in Table 2, and MS conditions are shown in Table 3. The conditions were set where n-1(5') co-eluted with FLP, while n-3(5') was separated (Fig. 1) to demonstrate impurity analysis performance.<h4>Conclusion</h4>The simulated sample consisting of mainly FLP and small amounts of n-1(5')/n-3(5') was analyzed using the LCMS-2050 and LabSolutions Insight Biologics software and obtained data were processed appropriately. Detection, identification, and quantitation at low concentrations were accomplished not only for n-3(5') well-separated from FLP but also for n-1(5') co-eluted with FLP. In case that establishing analytical conditions is difficult, Using this instrument setup and software will provide efficient quality confirmation of oligonucleotide therapeutics.<h3>3. Thermo Fisher Scientific: Simultaneous Quantitation and Untargeted Discovery (SQUAD) workflow using multiple fragmentation techniques for quantitation and characterization of trace level nitrosamine in biological matrices</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_squad_workflow_multiple_fragmentation_techniques_po_en_94a5b27b12.pdf">Full PDF for download</a></li></ul>N-Nitrosamines are a class of carcinogenic chemical compounds that pose threat to humans exogenously through matrices like drinking water, food, cosmetics, and endogenously by nitrosation in gastrointestinal tract, nitrate to nitrite conversion, etc. An accurate, reliable analytical method is critical for detection and quantification of these trace-level, polar and low molecular weight compounds to understand the association between their exposure and related diseases.&nbsp;An optimized SQUAD workflow is presented that discovers unknown compounds in the matrix by using high resolution orbitrap and a sensitive linear ion trap detector that quantifies the low intensity nitrosamines in a single run. The workflow uses multiple fragmentation types – HCD, CID and UVPD and MSn to help characterize isomeric structures.<h4>Methods</h4>Serial dilution of Nitrosamine standards were subjected to Simultaneous Quantitation and Discovery (SQUAD) analysis using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Thermo Scientific™ Vanquish™ Horizon UHPLC system</a> and the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/561">Thermo Scientific™ Orbitrap IQ-X™ Tribrid™ mass spectrometer</a> . Nitrosamine spiked matrices of human pooled urine and human Serum were subjected to SQUAD analysis.<h4>Data Analysis&nbsp;</h4>Spectra were evaluated for nitrosamines using Thermo Scientific™ FreeStyle™ software and annotated using Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™ 8.0 software</a>. Untargeted data analysis was performed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer 3.4 software</a>. Calibration curves were generated using Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">TraceFinder™ 5.2 software</a>.<h4>Conclusions&nbsp;</h4><ul><li>Detection limits as low ppb levels ( 0.2 ppb –0.5 ppb) and quantitation limits (0.2 ppb- 5 ppb ) in presence of the matrix were achieved for all seven nitrosamines by applying parallel ion trap analysis&nbsp;</li><li>SQUAD analysis was run on Orbitrap IQ-X Tribrid MS and achieved low ppb level quantitation in ion trap and identified unknown compounds through discovery analysis in Orbitrap.&nbsp;</li><li>Employing nano LC helped achieve lower limit of quantitation (5 ppt)</li></ul><h3>4. Waters Corporation: Validated LC-MS/MS Method for Vitamin E Acetate in Cannabis Plants and Derived Products</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009082_en_c7a6fc6190.pdf">Full PDF for download</a></li></ul><h5>Benefits&nbsp;</h5><ul><li>The validated LC-MS/MS method provides high sensitivity and specificity for VEA quantification in complex cannabis matrices, including flower and concentrates.&nbsp;</li><li>Minimal sample preparation reduces handling time and improves laboratory throughput, supporting efficient routine analysis.&nbsp;</li><li>The method demonstrates robust performance across multiple matrices, ensuring reliable detection even in lipid-rich, chemically diverse samples.</li><li>Use of Waters ACQUITY UPLC H-Class System and Xevo TQ-S micro Mass Spectrometer enables accurate quantitation well below regulatory requirements.&nbsp;</li><li>The validated method complies with Missouri DCR and DHSS regulatory limits, enabling laboratories to confidently monitor VEA in cannabis products.</li></ul>VEA is used as a diluent and viscosity modifier in certain cannabis-derived products, including vaping formulations. Following increased public health awareness during 2019, VEA became a marker of interest for quality control and regulatory surveillance across cannabis supply chains due to its potential impact on lung health. In addition to concentrates and vape liquids, trace residues may be present in infused products and processing intermediates. Therefore, reliable quantification of VEA in these chemically diverse, lipid-rich matrices requires an analytical method that is sensitive, selective, and robust against matrix effects. In February 2024, Missouri’s DCR revised the tolerance limit for VEA in cannabis products from 0.2 ppm to 5 ppm.1&nbsp;Liquid chromatography coupled with tandem quadrupole mass spectrometry (LC-MS/MS) provides the sensitivity and specificity required to distinguish VEA from endogenous cannabinoids, terpenes, and other lipophilic excipients, while also offering the sensitivity to support low-ppm and sub-ppm action limits. Unlike UVbased methods, where co-eluting chromophores may interfere, LC-MS/MS enables targeted multiple reaction monitoring (MRM) for confident identification and accurate quantitation in complex cannabis matrices.&nbsp;This application note describes the validation of an LC-MS/MS method for the quantitation of VEA in two inhalable products, cannabis flower and cannabis concentrates. The method was developed and validated using the Waters ACQUITY UPLC H-Class System coupled with a Xevo TQ-S micro Triple Quadrupole Mass Spectrometer. Chromatographic separation was achieved with a reversed-phase ACQUITY UPLC BEH C18 Column. A streamlined sample preparation approach was applied for cannabis flowers and concentrates, which minimizes handling time and increases sample throughput.&nbsp;The validation study was conducted at GPA. Method performance was evaluated for calibration range, linearity, LOD, LOQ, accuracy, precision, recovery, repeatability, and robustness. The results of these are evaluated against acceptance criteria set by State of Missouri DHSS and presented in this application note.<h4>Experimental&nbsp;</h4><ul><li>LC system: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198">ACQUITY UPLC H-Class Plus System&nbsp;</a></li><li>MS detection: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209">Xevo TQ-S micro Mass Spectrometer&nbsp;</a></li><li>Column: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1952">ACQUITY UPLC BEH C18 Column</a>, 2.1 x 50 mm, 1.7 µm (p/n: 186002350)</li><li>Software: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635">MassLynx™ 4.1 Software&nbsp;</a></li><li>Data processing: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=TargetLynx">TargetLynx™</a> Application</li></ul><h4>Conclusion&nbsp;</h4>The LC-MS/MS method developed and validated for VEA quantification in cannabis flower and concentrates demonstrates excellent linearity, accuracy, precision, and robustness, and meets the regulatory requirements. Calibration curves achieved R² values greater than 0.999, with LOD and LOQ well below the Missouri action limit of 5 ppm. Matrix-spiked recoveries across flower and concentrate samples were within the 70–130% acceptance range, and QC samples met all performance criteria, confirming method reliability. Robustness testing across analysts and matrices further validated the method’s reproducibility. Overall, this approach provides a streamlined, sensitive, and regulatory-compliant workflow for laboratories, enabling accurate monitoring of VEA to support quality control, consumer safety, and regulatory compliance in cannabis-derived products

News from LabRulezLCMS Library - Week 04, 2026

Article

Application

Academy

Health

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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SIELC Alltesta Power Tower for HPLC Alltesta

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free cloud software at hplc.cloud.

The Alltesta™ Power Tower acts as a power supply and chromatography data acquisition device, connecting, controlling, and streaming your Alltesta™ devices to our free <a href="https://lcms.labrulez.com/products/1250">cloud software</a> at <a href="https://hplc.cloud/">hplc.cloud</a>. It serves as a bridge between your laboratory and a centralized data storage hub. With the Power Tower and your free HPLC.cloud subscription, you can access your equipment and experiment results from anywhere, on any device, at any time, using any interface!
<h4>Specifications</h4>
<ul>
<li>Voltage Range: 80 ~ 264VAC</li>
<li>Rated Power: 140W</li>
<li>Dimensions (WHL) - 61 x 115 x 255 mm / 2.4 x 4.53 x 10.04 in</li>
<li>Weight: 1.05 kg / 2.3 lb</li>
<li>Sampling Rate: 20 Samples per second</li>
<li>Resolution: -1 to 2.5V that corresponds to -1000 to 2500mAU</li>
<li>Noise Level: Less than 0.05mAU</li>
<li>Sync Time: Chromatogram is synced with HPLC Cloud every second</li>
<li>Operating Temperature: 0 to 40° C, non-condensing</li>
</ul>
<h4>Ports</h4>
<ul>
<li>4 USB-A</li>
<li>4 Power outputs 24V, 2A</li>
<li>1 CAT5 ethernet connector</li>
<li>1 Galvanic isolated analog input -1V to +2.5V</li>
</ul>
<h4>Key Functionality</h4>
Start &amp; stop injections; analyze chromatograms; control the detector, valve, autosampler, and pump via HPLC.cloud
<ul>
<li>Real-time chromatogram streaming</li>
<li>Connect up to 4 Alltesta™ units via 4 USB ports</li>
<li>Build and record sequences</li>
<li>Create and store methods</li>
<li>Keep your sample information and analysis results in the cloud, never lose your data</li>
<li>Build calibrations from standards, then analyze and quantitate samples</li>
<li>Start, stop, and edit your analysis remotely from anywhere in the world</li>
<li>Access your data via PC, tablet, or mobile phone</li>
<li>Search your results online</li>
</ul>

SIELC HPLC Software - HPLC.cloud

SIELC has developed the first and only HPLC software that is accessible via the cloud.

<h5>Each <a href="https://lcms.labrulez.com/products?instrumentationItems=27&amp;search=SIELC">instrument</a> is connected directly to our secure cloud server via an Ethernet line.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>All instrument parameters are visible and adjustable on the fly from any internet-enabled device. Instrument settings are stored as methods, which can be retrieved and used for specific compounds.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_2_f626a8f395.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>Multiple samples can be organized in a sequence with various methods. Results can be compared and quantitated:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_3_6eb75a29c9.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
Please see an online example of the separation results <a href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.
<h5>With your Alltesta and HPLC.cloud free subscription, you can analyze almost any compound from the palm of your hand. For example, you can analyze the dyes that are in your favorite purple sports drink:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_4_b8e9abd33a.jpg" alt="SIELC: Separation of Blue 1 and Red 40 dyes in Gatorade Zero – Grape."/>
<h5>Alternatively, you can analyze the caffeine content in your morning coffee:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_5_f39557543e.jpg" alt="SIELC: Retention of Caffeine."/>
<h5>Another option is to separate and analyze the vitamin content in your daily multi-vitamin:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_6_bbf8e002a3.jpg" alt="SIELC: Separation of Vitamin C and Vitamin B2."/>

SIELC Samplify Automated sampling system

Samplify Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate

Samplify™ Is a device which allows unattended sampling of a reaction mixture or bioprocess or continue in line flow by taking small amount of liquid from the monitoring mixture and collecting it in a vial or a 96 wells plate. It also allows to dilute the sample with assigned dilution ratio and can provide reaction quenching to preserve the sample state at time of sampling. It has two main components: an autosampler and a probe. The Samplify™ &nbsp;controlled and setup by a PC software.<h3>Description</h3>It is a common task in the lab to periodically sample a reactor or a fermenter to obtain information about reaction kinetic, completion and have a tangible representation of intermediate states of a dynamic process. This usually done manually with pipettes or syringes, using stopwatch to measure time and requires to be in the lab during the entire process.With Samplify it can be done in automated mode. When an automated sampling process is used it increases accuracy, safety, and productivity. It reduces potential samples cross contamination, and exposure of the reaction mixture to oxygen or moisture, when anaerobic or moisture free conditions are required.<ul><li>Based on a proprietary alpha/beta mechanical configuration, Alltesta Miniature Autosampler and patent pending sample probe offers affordable and reliable automation for many analytical and liquid-handling applications.&nbsp;</li><li>Small controllable volume of sample can be programmed and precisely removed from the enclosed or open system. The sample will then be transferred to a vial with inert solvent with measured volume to create a controllable dilution factor.</li><li>A quench solution can be automatically preloaded in each vial to provide termination of reaction or biological process, and preserve sample content in the time of probing.</li><li>In order to effectively mix samples with diluents or quenching reagents a shaking capability of the autosampler tray can be initiated after each sample cycle.</li><li>PC based software allows quick, but flexible, setup of the sampling experiment. Several preloaded methods can be quickly adapted to a particular needs of the user.</li><li>For temperature sensitive samples the entire autosampler can be placed in the temperature controlled environment.</li><li>The device small size allow to place it close to the reactor and use short connection lines to minimize dispersion. Also a small cooler can be used to keep samples in cold conditions after they are collected.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg" alt="SIELC: SIELC Samplify™ Automated sampling system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Samplify_Automated_sampling_system_937b70e8d2.jpg 245w," sizes="100vw" width="1346" height="663"><h3>Specifications</h3><h4>Samplify™ Autosampler:</h4><ul><li>Syringe volume: 4.0 mL</li><li>Pressure: up to 250 psi</li><li>Valve configuration: 7×6</li><li>Power Requirements: 24 VDC</li><li>Current Requirements: Up to 1.0 A</li><li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li><li>Weight: &nbsp;3 kg / 6.6 lb</li><li>Inlets/Outlets: Thread 10-32 UNF compression type</li><li>Materials in contact with liquid: SS316, PTFE, PEEK</li><li>Tray capacity: 48 vials 1.8 mL volume or 96 wells plate</li><li>Communication: USB virtual serial port</li></ul><h4>Samplify™ Probe:</h4><ul><li>Connection: requires Alltesta-Samplify autosampler</li><li>Dimensions: 1 x 1 inch, DIA ¼ inch. Immersion length up to 6 inch</li><li>Materials in contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 µL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: &nbsp;200 °C</li></ul><h3>Software</h3>The Samplify can be controlled:<ul><li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li><li>remotely through our cloud software (autosampler.cloud),</li><li>directly via our Samplify PC software, allowing to fully control all the parameters of the sampling operations</li></ul><h3>Available Custom Options</h3>Metal-free version of the syringe pump includes no metal contact pump head.

PBI Barocycler 2320EXT Sample Preparation System

The Barocycler 2320EXT is a compact, bench top instrument that can process up to 16 samples simultaneously using PCT MicroTubes.

<h4>For the PCT-HD Sample Preparation System</h4>Pressure Cycling Technology (PCT) is a unique, patented technology platform based on repeated cycles of hydrostatic pressure between ambient (14.7 psi) and ultra-high levels. These rapid changes in pressure are used to control biomolecular interactions for applications such as accelerated proteolytic digestion, and improved extraction of cellular components, including proteins and lipids. The PCT platform allows for a high degree of safety, speed, reproducibility, and convenience in modern sample preparation protocols.The PCT-HD Sample Preparation System is a proven PCT-based workflow for processing small tissue samples for proteomic and other applications. PCT-HD combines efficient, hands-off tissue homogenization and protein extraction with pressure-enhanced digestion, for rapid, efficient and reproducible generation of peptides for MS analysis. This unique workflow combines two of PBI’s innovative sample preparation tools: the Barocycler 2320EXT and our patentpending PCT MicroPestles. PCT-HD and the Barocycler 2320EXT can provide unprecedented speed and reproducibility for biomarker discovery, yielding peptides ready for clean-up and MS within 2-4 hours from the start of tissue processing.The Barocycler 2320EXT is a compact, bench top instrument that can process up to 16 samples simultaneously using PCT MicroTubes. Features that come standard with the 2320EXT include: data input and output options to accommodate validation and quality control; computer-operated control with touch screen programming and automatic data logging; and the ability to control multiple pressure cycling parameters, such as the rate of pressure increase/decrease, both the high and low pressure levels, and the shape of the pressure profile (e.g., sine wave, square wave, and others). In addition, the 2320EXT is available with a choice of two different methods for temperature control (an external circulating waterbath for heating and cooling, or a built-in electric heater).<blockquote>Patent Pending</blockquote><h4>The Barocycler 2320EXT</h4><ul><li>Computer-operated control with Touch Screen Interface</li><li>Pressure Range: ambient - 45,000psi (310 MPa)</li><li>Choice of temperature control - heating/cooling or heating only</li><li>Max Temperature: 95˚C</li><li>Min Temperature: 4˚C with circulating waterbath, ambient with electric heating</li><li>Insulated closure for easier use at high temperatures</li><li>Chamber capacity: up to 16 samples in MicroTubes</li><li>Easily accessible USB port on front panel</li><li>Requires 110psi input air pressure</li><li>Input air pressure can be provided by an air compressor, house air or nitrogen lines, air or nitrogen tanks.</li><li>Pressure medium: Distilled Water</li></ul><h4>Touch Screen Interface</h4>Pressure Profiles are programmed via an onboard touch screen, which can be connected to a network. The instrument is also equipped with a USB port that can be used to connect a mouse or keyboard to the 2320EXT.<ul><li>Pressure Mode Settings: Barocycler, Ramp, Waveform</li><li>Cycled or Static Pressure</li><li>Network Enabled</li><li>Automatic Logging of All Run Parameters</li><li>Data Output&nbsp;</li><li>Save and Record Programs</li><li>Real-time pressure display graph shows pressure and temperature throughout the run</li><li>Touch Screen Temperature Control (with electric heating option only)</li></ul><h4>MicroTubes and MicroPestles</h4>The 2320EXT comes with a full MicroTube Adapter Kit, that includes the MicroTube Cartridges, Capper Tool, and other accessories that are required when using PCT MicroTubes in the 2320EXT. PCT μPestles, used in conjunction with PCT MicroTubes in the Barocycler, are designed for rapid, hands-off homogenization and extraction when working with minute amounts of solid tissue. PCT MicroTubes and MicroPestles are an integral part of the PCT-HD workflow.<ul><li>Process up to 3mg of solid tissue in 30uL of extraction reagent</li><li>Process as few as 5 x 104 cells</li><li>MicroTubes are constructed of non-wetting, low binding FEP plastic</li><li>MicroPestles are made from inert PTFE (Teflon)</li></ul>

Trajan Mitra Microsampling Devices - Specimen bag

The Specimen Bag Mitra format is perfect for those looking to create their own collection kits for remote sampling or maintain bulk devices for centralized sampling in clinical or field settings.

<h3>About</h3>The Specimen Bag Mitra format is perfect for those looking to create their own collection kits for remote sampling or maintain bulk devices for centralized sampling in clinical or field settings.Each Specimen Bag is sealed and includes a barcoded Mitra device with a unique identifier and desiccant. After collection, the device is placed back into the Specimen Bag to facilitate quick drying and protect the sample from external contamination.If you need logistics help, bioanalytical lab services, or kitting support, we can connect you with experienced organizations that specialize in microsampling.<h4>Build Your Own Kits or Sample Large Cohorts Remotely</h4>With this format you can collect many samples in the field and ship them back to the lab in bulk. You may prefer to insert them into your own all-in-one kit.<ul><li>10, 20, &amp; 30 µL volumes</li><li>Protective outer casing</li><li>Barcoding to manage chain of custody</li><li>Packed in specimen bag with desiccant for transport &amp; storage</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17218576460536_2"><ul><li>Indication for Use: A single-use, non-sterile device used as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses.&nbsp;</li><li>Sampler Tip Volume: 10, 20, or 30uL&nbsp;&nbsp;</li><li># of Samplers per Device: 2 or 4&nbsp;</li><li>Shelf Life: 18 months&nbsp;</li><li># Device per pack: 30/Pk</li><li>Pack content: Mitra Device, Specimen Bag with Desiccant, Sample ID Barcode, 1x IFU</li><li>Regulatory status: Mitra® devices are CE-IVD (IVDR) devices intended as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses. They are available as registered IVD Devices in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, Mitra devices are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices for the particular diagnostic testing intended.</li></ul><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Mitra%20Device%20Specimen%20Bag/VLBL-0125-Rrev00.pdf">Instructions for Use (RUO)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Mitra%20Device%20Specimen%20Bag/VLBL-0125rev00.pdf">Instructions for Use (MD)</a></li></ul><h3>Resources</h3><ul style="list-style-type:disc;"><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_2022%20Mitra%20Collateral/Mitra%20Specification%20Sheet%20(3).pdf">Mitra Device Specification Sheet</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/User%20Guides/Mitra%20User%20Guide%20Extracting%20&amp;%20Processing%20Microsamples.pdf">User Guide | How to Extract &amp; Process Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/GenTegraRNA-NEO%20for%20Mitra%20devices%20USER%20GUIDE%2011.15.23.pdf">User Guide | How to Stabilizer RNA on Mitra Microsamples with GenTegraRNA-NEO™</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions?hsLang=en">Frequently Asked Questions</a></li></ul><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3></div>Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases</li></ul>

Trajan Mitra Microsampling Devices - Microsample collection kit

The Microsample Collection Kit Mitra format streamlines the blood microsampling process, enabling both assisted and self-collection at home or in the field.

<h3>About</h3>The Microsample Collection Kit Mitra format streamlines the blood microsampling process, enabling both assisted and self-collection at home or in the field. Each kit is equipped with device, barcoding for secure chain of custody, easy-to-follow instructions, all necessary finger-prick sampling supplies, and a return-mailing envelope, all packaged for global distribution. Ideal for remote blood collection, these kits ensure that samples can be directly sent to accredited labs for precise analysis.For customization to meet the demands of large-scale projects or to optimize your workflows and logistics, please consult with our <a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/contact">Microsampling Consultants</a>. They will provide tailored recommendations to align with your project’s specific objectives.<h4>All-in-One Solution for Remote Specimen Collection</h4>Our Mitra devices can be packed in convenient kits with sterile components for safe sample collection. Kits can be shipped to your end-users.<ul><li>10, 20, &amp; 30 µL volumes</li><li>Protective outer casing</li><li>Barcoding to manage chain of custody</li><li>Packed in specimen bag with desiccant for transport &amp; storage</li><li>Contains devices &amp; supplies</li><li>Includes instructions for use &amp; return shipping envelope</li><li>Sized for easy global mailing</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_1721774838869_2"><ul><li>Indication for Use: The Microsample Collection Kit is a single-use non-sterile procedure pack intended for the collection, storage and transport of capillary whole blood specimens of specified volume.</li><li>Sampler Tip Volume: 10, 20, or 30uL</li><li># of Samplers per Device: 2 or 4</li><li># Device per kit: 1/Pk</li><li>Pack content: 1x Mitra Device, 1x Specimen Bag with Desiccant, Sample ID Barcode, Lancet (x2 or x4), Gauze (x1 or x2), Bandage (x2 or x4), 1x Plastic Shipping Envelope, 1x IFU</li><li>Shelf Life: 18 months</li><li>Regulatory status: The Mitra Blood Sample Collection Kit is a procedure pack. The kits are available as registered Medical Device in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, procedure packs are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices and Mitra Microsample collection kit for the particular diagnostic testing intended.</li></ul></div><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Collection%20Kits/VLBL-0124-Rrev00.pdf">Instructions for Use (RUO)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/Collection%20Kits/VLBL-0124rev00.pdf">Instructions for Use (MD)</a></li></ul><h3>Resources</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_2022%20Mitra%20Collateral/Mitra%20Specification%20Sheet%20(3).pdf">Mitra Device Specification Sheet</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/Guidelines%20for%20Collecting%20Samples%20at%20Home-1.pdf">Guidelines for Collecting Samples at Home</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions">Frequently Asked Questions</a></li></ul><div class="tabs__tab txt--light tabs__tab--active" id="tab_1721774838869_4"><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3>Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases</li></ul></div>

Trajan Mitra Microsampling Devices - 96-autorack

The 96-Autorack™ format is designed for laboratories to efficiently batch and process Mitra microsamples using a standard 96-well format.

<h3>About</h3>The 96-Autorack™ format is designed for laboratories to efficiently batch and process Mitra microsamples using a standard 96-well format. Compatible with most 1mL &amp; 2mL collection plates and 96-channel pipettors &amp; liquid handling workstations, the 96-Autorack enhances lab productivity and integration. We provide two options: an empty 96-Autorack, perfect for accessioning microsamples from Mitra devices received from the field for building plates, extractions, and long-term storage; and prefilled 96-Autoracks with 96 Mitra samplers, ideal for method development and handling calibrators and QCs.<h4>Accession &amp; Process Multiple Mitra Samples at Once</h4>This high-throughput solution allows you to rapidly process Mitra microsamples for method development, plate building, extractions - or all of the above!<ul><li>Racks available empty or filled with 96 microsamplers (10, 20, &amp; 30 µL volumes)</li><li>Filled racks come with a 2mL deep well storage plate</li><li>Compatible with 96-channel pipettors &amp; liquid-handling workstations</li><li>Options with &amp; without barcoding</li></ul><h3>Specifications</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_2"><ul><li>Indication for Use: A single-use, non-sterile device used as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses.&nbsp;</li><li>Sampler Tip Volume: 10, 20, or 30uL&nbsp;&nbsp;</li><li># of Samplers per Device: 0 or 96&nbsp;</li><li># Device per pack: 1/Pk</li><li>Pack content: 1x 96-Autorack (empty or pre-filled), Sample ID Barcode, 1x Collection Plate, 1x IFU</li><li>Shelf Life: 18 months&nbsp;</li><li>Regulatory Status: Mitra® devices are CE-IVD (IVDR) devices intended as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses. They are available as registered IVD Devices in the European Union and United Kingdom, Australia, Brazil, China, and Canada, as well as multiple Health Ministries worldwide. In the USA, Mitra devices are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices for the particular diagnostic testing intended.</li></ul><h3>How to Use</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Mitra%20IFUs/2024/VLBL-0098rev04.pdf">96-Autorack Instructions For Use (IVD)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/IFUs/2023/96%20autorack/VLBL-0098-Rrev01.pdf">96-Autorack Instructions For Use (RUO)</a></li></ul><h3>Resources</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/User%20Guides/Mitra%20User%20Guide%20Extracting%20&amp;%20Processing%20Microsamples.pdf">User Guide | How to Extract &amp; Process Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/NEOX_Content/Technical%20Briefs%20and%20Guidances/Long%20&amp;%20Short%20Term%20Storage%20of%20Mitra%20Samples_TG00010122.pdf">User Guide | How to Store Dried Mitra Microsamples</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/hubfs/GenTegraRNA-NEO%20for%20Mitra%20devices%20USER%20GUIDE%2011.15.23.pdf">User Guide | How to Stabilizer RNA on Mitra Microsamples with GenTegraRNA-NEO™</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.neoteryx.com/frequently-asked-questions?hsLang=en">Frequently Asked Questions</a></li></ul><h3>Mitra devices deliver tremendous ease &amp; value to specimen collection!</h3><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_3"><div class="tabs__tab txt--light tabs__tab--active" id="tab_17217760014256_4">Mitra® Microsampling Devices offer a streamlined, flexible, and decentralized approach to biological specimen collection. Enjoy the benefits of easier trial recruitment, increased adherence / compliance, elimination of cold-chain shipping / storage &amp; reduced reliance on trained staff.<h4>The gold-standard in remote specimen collection</h4>Powered by VAMS® technology, the patented Mitra® Microsampling Device offers a streamlined, flexible, and decentralized approach to biological specimen collection, transport, and preparation. Use Mitra to facilitate sampling direct from animals, humans, or tubes to collect precise, reliable dried microsamples compatible with a wide variety of downstream applications as evidenced by the hundreds of published peer-reviewed manuscripts and the millions of devices that have been used in the field.<h4>Mitra: pioneering VAMS technology</h4>Trajan’s proprietary VAMS® Technology is at the heart of the Mitra device, featuring a sampler tip crafted from a porous, hydrophilic polymeric material. This 'precision sponge' rapidly and consistently absorbs a specific volume of biological fluid—simply by contacting the source. The fluid is then allowed to dry acting as a stabilizer for most analytes.Available in three tip sizes—10, 20, and 30 µL— and multiple formats, Mitra devices meet diverse end-user and analytical needs. Configurations include dual (2) or quad (4) samplers across various formats.&nbsp; Collect from 20 µL to 120 µL per device based on format selected. Our team of expert microsampling consultants is on hand to provide scientific support, helping you choose the optimal format and workflow for your specific applications.<ul><li>Reliable: Quantitative sampling with pipette-like precision (RSD ≤ 5%). VAMS eliminates traditional blood hematocrit biases and results in extremely low in-lab sample rejection rates. No glue used in assembly, no matrix interferences.</li><li>Versatile: Collect biological fluids—blood, plasma, urine, saliva, and even tears—efficiently from humans and animals (e.g., finger, arm, knee, tail, etc.) or containers. Manual to fully automated workflows are available.</li><li>User-Friendly: 99% sample acceptance rate validated by studies. VAMS tip acts as a fill indicator to confirm successful sampling. Outer housing protects against contamination and facilitates easy remote or home sampling.</li><li>Cost-Effective: Eliminate phlebotomy, couriers, and cold-chain shipping / storage requirements. Devices are compatible with standard automated liquid handling systems and available in various formats to suit different budgets.</li></ul><h4>Successful uses</h4>Have you seen the published, 3rd party literature of how others have successfully implemented Mitra devices?<ul><li>Preclinical</li><li>Clinical Trials / Research</li><li>Therapeutic Drug Monitoring</li><li>Omics</li><li>Biomonitoring</li><li>Toxicology</li><li>Hormones / Steroids</li><li>Biomarkers</li><li>Vitamins</li><li>Allergens</li><li>Population Screening</li><li>Infectious Diseases&nbsp;</li></ul></div></div></div>

Quantitative analysis of proteins (Bioanalytic) 

In our laboratory, proteomic analyses, including quantitative analysis of proteins, are performed by HPLC-MS or HPLC-MS/MS methods on a ZenoTOF 7600 high-resolution mass spectrometer (SCIEX).

Quantitative analysis of proteins is important in many industries and applications. Proteins include a diverse group of compounds that participate in virtually every biological process. Proteins are building blocks, hormones, transport molecules, cell receptors, cell growth and differentiation factors. Because of this, the analysis of protein interactions is crucial in drug design and production.Proteins are macromolecules - the basis of their structure are polypeptide chains containing from 100 to more than 10000 amino acids (10 - 100 kDa). Proteins are found in simple and complex forms. Complex proteins contain a non-protein component (sugar, lipids, metal ions or dyes) in their structure.In our laboratory, proteomic analyses, including quantitative analysis of proteins, are performed by HPLC-MS or HPLC-MS/MS methods on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=ZenoTOF+7600">ZenoTOF 7600</a> high-resolution mass spectrometer (SCIEX). We process the obtained results using Sciex OS, Spectronaut, Skyline software.<h4>MS vs MS/MS</h4>Quantitative analysis of intact protein (whole protein in intact form) leads to the concentration (determined from a prepared curve from a standard (with or without matrix) and confirmation of the identification by superimposing (on a mirrored basis) the MS spectrum of the protein determined in the sample to the MS spectrum of the standard. For your needs, we can generate a list of protein-structured compounds (impurities) that were identified in the sample and determine the ratio of their signal area to the signal area of the analyzed protein.The result of proteomic quantitative peptide analysis allows precise and much more sensitive determination of protein concentration. The analysis is based on MS/MS data obtained for peptides obtained by digestion with proteolytic enzymes. The reported result consists of the concentration of the protein under study and confirmation of its presence based on the determined amino acid sequence. For such analyses, it is recommended to add standards internal standards.<blockquote>Our offer:<ul><li>For proteins and peptides with low masses - intact analysis with MS detection;</li><li>Proteins with masses &gt;20kDa require optimization of the digestion method and MS/MS detection. This results in specific signals from the analyzed protein in the mixture/matrix;</li><li>From complex matrices (including serum, cell culture, fungal suspension), sample analysis involves several steps:&nbsp;<ul><li>Sample purification;</li><li>Digestion to obtain unique peptides to distinguish the analyzed proteins from other proteins in the sample;</li><li>Sample preparation requires appropriate method optimization and validation (let's set validation criteria individually);</li></ul></li><li>Reported quantitative result includes confirmation of the presence of the protein in native form or a peptide derived from it (usually confirmation includes at least two peptide sequences);</li><li>Statistical analysis using tools: MarkerView (PCA), GraphPad Prism, Trends, Sciex OS.</li></ul>To optimize and validate the method, it is necessary to provide a standard, in the case of proteins with high masses - it is recommended to add an internal standard.See also:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/service/identyfikacja-bialek/">Protein identification</a>,</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1296">Analysis and characterization of monoclonal antibodies</a>.</li></ul></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_analysis_of_proteins_1_de494646f2.jpg" alt="Bioanalytic: Quantitative analysis of proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_analysis_of_proteins_1_de494646f2.jpg 200w," sizes="100vw" width="777" height="606">

Identification of the substance – what is in your sample? (Bioanalytic)

We are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.

<h4>Substance Identification - Looking for answers to what's in your sample? - we can help you.</h4>Substance identification based on LC-MS/MS techniques can involve single compounds or mixtures of substances. In our analyses, we rely primarily on the knowledge and skills we have acquired after many years of work. For analysis, we use full laboratory facilities for sample preparation and high-resolution mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/o-nas/">equipment</a>).We use different work algorithms depending on the material we are dealing with and the analytical task to be solved. We use DDA (IDA) or DIA (SWATH) methods or targeted approaches (e.g. MRMhr) if we know what to expect. Thus, we are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg 245w," sizes="100vw" width="1881" height="1156"><h4>COST OF SERVICE</h4>We determine the cost of the service individually. The final cost is influenced by many factors related to the nature of the sample itself and the client's expectations. Thus, the price depends, among other things, on the complexity of the sample preparation procedure, the scope and number of analyses, etc.The service includes (depending on individual arrangements):<h5>Development of sample preparation procedure</h5><ul><li>Depending on the matrix and test substances.</li></ul><h5>Development of analytical methods</h5><ul><li>Application of high-resolution LC-MS/MS analyses using DDA (IDA) or DIA (SWATH) experiments and software to support the development of targeted screening methods.</li></ul><h5>Analysis of results includes:</h5><ul><li>finding and identifying the substances of interest, interpreting MS/MS spectra and proposing a structural formula.</li><li>Comparative analysis of sample series, e.g. PCA,</li><li>profiling of analytes in sample series</li></ul><h5>Reporting of results</h5><ul><li>Compile results into easy-to-read reports.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg 245w," sizes="100vw" width="1919" height="1208">

Characteristics of monoclonal antibodies (Bioanalytic)

At LabExperts, proteomic analyses are performed on a ZenoTOF 7600 high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation.

Antibodies are molecules made up of 4 polypeptide chains: two heavy (H - high-chain) linked to each other by a specific type of chemical bond - a disulfide bridge, and two light (L - light-chain) linked by an identical bond to the heavy chains. Monoclonal antibodies (mABs) are used in various forms - as free molecules, in the form of a drug-antibody combination, and in the form of an immunoconjugate, a drug-antibody-biopolymer combination.Their main use is in highly specific targeted therapy for cancer, cardiovascular, autoimmune and nervous system diseases. They are also used in the treatment of osteoporosis, Alzheimer's disease or transplantation. The range of applications of monoclonal antibodies is systematically expanded. The introduction of a new antibody to the market requires complex product characterization, which involves numerous proteomic analyses, which are performed by LC-MS/MS techniques, among others.<h4>LC-MS/MS</h4>The characterization of monoclonal antibodies involves many aspects, including, but not limited to, a suite of analyses related to confirmation of the structure, chain sequence and degree of modification of the antibody. Post-translational modifications (PTMs) are chemical changes that occur after the translation process. These changes generally result in an increase in the immunogenicity of the drug. For this reason, it is essential to monitor modifications as critical quality attributes (CQAs) during both the production and storage of the drug. To ensure monitoring of CQA points, the primary structure of the protein is characterized based on peptide mapping (peptide mapping) analysis. The protein under study is subjected to proteolytic digestion using enzymes such as trypsin, chymotrypsin, Lys-C, Glu-C. As a result of various proteolytic enzymes, a wide range of peptides is obtained. Ultimately, coverage of the entire sequence of the protein under study is obtained.<figure class="image"><img style="aspect-ratio:1536/947;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg" alt="Bioanalytic: Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg 245w," sizes="100vw" width="1536" height="947"></figure>At LabExperts, proteomic analyses are performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=ZenoTOF+7600">ZenoTOF 7600</a> high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation. This provides us with data for unambiguous identification of protein-building amino acids. We process the results using Sciex OS, Skyline and Spectronaut software.<blockquote>We perform:<ul><li>Whole protein analyses - Intact - to determine the exact mass and confirm the theoretical mass;</li><li>N-glycosylation profile determination (based on MS spectrum reconstruction):<ul><li>Glycosylated form;</li><li>Deglycosylated;</li><li>Glycosylated with reduced disulfide bridges;</li><li>Deglycosylated with reduced disulfide bridges.</li></ul></li><li>Peptide maps:<ul><li>Protein digestion with various proteases so that sequence coverage is close to 100%<ul><li>Analysis of PTMs:</li><li>Location of modifications</li></ul></li><li>[%] of modifications compared to the unmodified sequence</li></ul></li><li>TiPOS testing - (technical proof of similarity) - technical confirmation of the similarity of the protein synthesized by the customer (from several LOTs) with the target protein (provided as a template).</li></ul>In order to maintain the highest possible analytical standards, we perform monoclonal antibody analyses under the regime of an internally developed and implemented laboratory quality system based on GLP principles.See also our other proteomics services: <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/service/identyfikacja-bialek/">Protein identification</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1294">Protein quantitative analysis</a></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg 245w," sizes="100vw" width="1536" height="908"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg 245w," sizes="100vw" width="1536" height="858">

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