<p><strong>In the week of February 16, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Agilent Technologies: From Complex to Simplicity – Get More Peace of Mind on Your HPLC Workflows with InfinityLab Assist</strong></h5><ul><li>Mo, 16.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5908">Registration</a></li></ul><p>Discover how Agilent InfinityLab Assist simplifies LC system control with intuitive multitouch operation, guided troubleshooting, and smart monitoring to boost efficiency and reduce downtime.</p><h5><strong>2. Thermo Fisher Scientific: Driving the Process Development of Recombinant Therapeutic Products with Automated MS-Based Target Peptide Mapping</strong></h5><ul><li>Tu, 17.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5931">Registration</a></li></ul><p>Explore automated MS-based MAM workflows for peptide mapping, enabling faster CMC decisions and scalable analytics from development to QC readiness.</p><h5><strong>3. Thermo Fisher Scientific: PFAS Analysis Solutions – Innovations for Today’s Labs</strong></h5><ul><li>Tu, 17.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5818">Registration</a></li></ul><p>Explore the latest challenges and solutions in PFAS analysis for environmental and food testing, including advanced workflows that improve sensitivity, accuracy, and lab efficiency.</p><h5><strong>4. 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Thermo Fisher Scientific: Single Cell and Immunopeptidomics Explorations on Orbitrap Astral MS</strong></h5><ul><li>Th, 19.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5932">Registration</a></li></ul><p>Discover how Orbitrap Astral MS and Vanquish Neo HPLC enable deep single-cell proteomics and immunopeptidomics from ultra-low-input clinical samples.</p><h5><strong>9. Evosep Biosystems: Experimental Design Considerations for Scalable Proteomics</strong></h5><ul><li>Th, 19.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5836">Registration</a></li></ul><p>Explore best practices for experimental design in LC-MS–based proteomics to build robust, reproducible, and scalable analytical workflows.</p><h5><strong>10. Waters Corporation: Ask Me Anything About Managing Computerized System Risks to Data Integrity</strong></h5><ul><li>Th, 19.2.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5809">Registration</a></li></ul><p>Learn practical strategies to manage computerized system risks in regulated laboratories and protect data integrity while ensuring compliance.</p><h5><strong>11. Shimadzu Corporation: Automating a Comprehensive Amino Acid Panel for Inborn Errors of Metabolism</strong></h5><ul><li>Th, 19.2.2026 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5936">Registration</a></li></ul><p>Discover how Shimadzu’s CLAM-2040 automates LC-MS/MS sample preparation to standardize workflows, reduce manual handling, and improve consistency in clinical labs.</p>

Webinars LabRulezLCMS Week 08/2026

Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry

<p><strong>Metalloproteins play essential roles in marine phytoplankton, yet their metalloproteomes remain poorly characterized. This study combines protein modeling, quantitative proteomics, and ICP-MS measurements to assess metal requirements across several phytoplankton species, including chlorophytes, a haptophyte, and a cyanobacterium.</strong></p><p>Results show strong agreement between proteome-inferred metal demands and measured cellular metal quotas, while providing deeper insight into biological functions. Mg-containing proteins were most abundant overall, with Zn, Fe, and Mn as dominant trace cofactors. Distinct differences between prokaryotic and eukaryotic phytoplankton were observed, and strain-level analysis demonstrated how metalloproteomes reflect environmental adaptation and metal limitation strategies.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233"><strong>Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes</strong></a></h4><p>Qiong Zhang*, Jiayou Ge, Fengjie Liu, Shabaz Mohammed, Kedong Yin, and Rosalind E. M. Rickaby</p><p>Environ. Sci. Technol. 2025, 59, 50, 27367–27377</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.est.5c11233">https://doi.org/10.1021/acs.est.5c11233</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><p>Many important biological processes of phytoplankton, including photosynthesis, carbon fixation, and nitrogen reduction, rely on enzymes that have metals as cofactors. <a href="javascript:void(0);">(1)</a> It has been estimated that half of all enzymes must be associated with a particular metal to function. <a href="javascript:void(0);">(2)</a> The availability of metals in the environment has changed over geological time and, given imperfections in the selectivity of metal transport systems, proteins have evolved to make the best use of metals that are more available. <a href="javascript:void(0);">(3−5)</a> Depending on their evolutionary histories, different groups of marine phytoplankton may have differences in their metalloprotein compositions and metal transport strategies. The evolving trace metal availability in the environment, thus, may provide a selective pressure to promote the proliferation of different phytoplankton groups in the oceanic environment, affecting the phytoplankton community structure with knock-on effects on the marine food web and carbon sequestration. <a href="javascript:void(0);">(5)</a> It is crucial to understand the utilization of trace metals by different groups of marine phytoplankton that inhabit contrasting niches in the modern ocean, not only by assessing the accumulation of trace metals in cells but also examining the functions of trace metals in different biological pathways.</p><p>In previous studies, the utilization or requirements of trace metals by marine phytoplankton were mostly determined by measuring the elemental stoichiometry in cells (metallome or quota of trace metals). <a href="javascript:void(0);">(6−8)</a> In the field, attempts have also been made to investigate the elements in phytoplankton <a href="javascript:void(0);">(9,10)</a> which have then been compared with profiles in the abiotic environment to understand their relationships. <a href="javascript:void(0);">(11)</a> The discovery of various metal storage proteins and the plasticity in elemental quotas under different environmental conditions raise the question of whether intracellular trace metal quotas reflect true differences in trace metal usage by phytoplankton. <a href="javascript:void(0);">(12,13)</a> In the past few decades, more and more studies have focused on metal–protein partnerships, using approaches such as genome-wide analysis to identify homologues of known metalloproteins and other deduced metal-binding motifs encoded within sequenced genomes. <a href="javascript:void(0);">(5,14)</a> However, this approach has limitations in estimating metal abundance in cells because not all genes predicted in the genome would be translated and expressed as proteins under all environments, and a single gene for a metalloprotein could be highly expressed, such that a translation from genetic presence to a required concentration is not linear and could be environment-dependent. To obtain <i>in situ</i> metal abundance in metalloproteins in cells, native separation of proteins by HPLC or PAGE followed by the detection of metals using ICP-MS <a href="javascript:void(0);">(15,16)</a> has been used. This approach also has limitations in that, without bioinformatics, it cannot identify the function of proteins within which the metals are found, and it may also depend on the composition of the media to which the organism has been exposed and in which it has been growing. An ideal way to solve the problem would be to separate and purify each of the proteins and then identify both the associated metal (<i>e.g.,</i> by ICP-MS) and the protein sequence (<i>e.g.,</i> by mass spectrometry). However, this approach is constrained by the resolution of chromatographic or electrophoretic techniques, making it challenging to establish the metal–protein associations unambiguously; many proteins often co-occur in metal-containing fractions. Additionally, this method requires huge volumes of biomass and is both time-consuming and complex, complicating the reconstruction of the expressed metalloproteome in cells. The techniques and challenges for metalloproteomics have been discussed in detail in previously published review articles (and the references therein). <a href="javascript:void(0);">(17)</a></p><p>In this study, we determined the trace metal utilization (the metallome) of different phytoplankton from their expressed proteome via a protein modeling and homologue identification approach, and then compared the resulting metallome with the metal quota data measured by the traditional inductively coupled plasma mass spectrometry (ICP-MS). This approach allows the building of a link between the truly used trace elements and their biological functions in phytoplankton. We aim to fill the gap between an estimation of metals encoded in the genome and metal analysis by ICP-MS in phytoplankton, which may be more broadly applicable to infer cellular metallomes.</p><h3><strong>Materials and Methods</strong></h3><h4><strong>ICP-MS Analysis</strong></h4><p>All preparation of samples for ICP-MS measurement was undertaken using trace metal clean methods. All plasticware used in this study, including culture flasks, pipette tips, and centrifuge tubes, was precleaned in 10% quartz-distilled HCl and thoroughly rinsed with 18 MΩ cm water in a clean laboratory. Trace elements in the filtered lysates of <i>G. huxleyi</i> (OA1, OA4, OA8, OA15, OA16, OA23, and RCC 1242) and <i>Synechocystis</i> (FACHB898) strains were measured using an ICP-MS. The detailed ICP-MS method and instrumental settings are described in Zhang et al. (2018). <a href="javascript:void(0);">(31)</a></p><h4><strong>Protein Mass Spectrometry</strong></h4><p>The peptides were resuspended in 5% formic acid and 5% DMSO prior to mass spectrometry. They were separated on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=Ultimate+3000+UHPLC"><strong>Ultimate 3000 UHPLC </strong></a>system (Thermo Fisher Scientific) and electrosprayed directly into a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/559"><strong>Q-Exactive Mass Spectrometer</strong></a> (Thermo Fisher Scientific) through an EASY-Spray nanoelectrospray ion source (Thermo Fisher Scientific). The peptides were trapped on a C18 PepMap100 precolumn (300 μm i.d. × 5 mm, 100 Å, Thermo Fisher Scientific) using solvent A (0.1% formic acid in water) at a pressure of 500 bar. The peptides were separated on an in-house packed analytical column (75 μm i.d. × 50 cm packed with ReproSil-Pur 120 C18-AQ, 1.9 μm, 120 Å, Dr. Maisch GmbH) using a linear gradient (length: 65 min, 15% to 35% solvent B [0.1% formic acid in acetonitrile], flow rate: 200 nL/min). The raw data were acquired on the mass spectrometer in a data-dependent mode (DDA). Full scan MS spectra were acquired in the Orbitrap (scan range 350–1500 <i>m</i>/<i>z</i>, resolution 70000, AGC target 3e6, maximum injection time 50 ms). After the MS scans, the 10 most intense peaks were selected for HCD fragmentation at 30% of normalized collision energy. HCD spectra were also acquired in the Orbitrap (resolution 17500, AGC target 5e4, maximum injection time 120 ms, isolation window 1.5 <i>m</i>/<i>z</i>) with the first fixed mass at 180 <i>m</i>/<i>z</i>. Charge exclusion was selected for unassigned and 1+ ions. The dynamic exclusion was set to 20 s.</p><h3><strong>Results and Discussion</strong></h3><h4><strong>Trace Elements Determined from the Expressed Proteomes of Different Phytoplankton</strong></h4><p>Based on the expressed proteome sequences, we predicted metal-binding proteins and compared their distribution among various phytoplankton species (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig2">Figure 2</a>). The inference revealed 28 different metals in <i>G. huxleyi</i>, 31 different metals in <i>C. reinhardtii</i>, and 30 different metals in <i>Synechocystis</i>. Although only 6 different metals were identified in <i>O. tauri</i> proteins, it has to be noted that the analysis for <i>O. tauri</i> was only based on the 40 most abundant proteins reported in this organism <a href="javascript:void(0);">(21)</a> and, hence, the listed elements, including Mg, Fe, Mn, Ca, Zn, and Cu, may represent the most essential metals for the species.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Environ_Sci_Technol_2025_59_50_27367_27377_Figure_2_The_number_of_metal_binding_proteins_identified_in_the_expressed_proteome_of_the_haptophyte_G_9b8ac0f004.jpeg" alt="Environ. Sci. Technol. 2025, 59, 50, 27367–27377: Figure 2. The number of metal-binding proteins identified in the expressed proteome of the haptophyte G. huxleyi (a), the chlorophyte C. reinhardtii (b) and O. tauri(c), and the cyanobacteria Synechocystis (d). Pie charts show the percentages of metalloproteins containing specific metals. Original proteome data for G. huxleyi are from Mckew et al. (2013) (24) for C. reinhardtii are from Hsieh et al. (2013) (77) for O.tauriare from Martin et al. (2012) (21) for Synechocystisare from Chen et al. (2018). (22) *Noted that for O. tauri, only the 40 most abundant proteins were included in this analysis. ND: no metal binding was found in the proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Environ_Sci_Technol_2025_59_50_27367_27377_Figure_2_The_number_of_metal_binding_proteins_identified_in_the_expressed_proteome_of_the_haptophyte_G_9b8ac0f004.jpeg 123w," sizes="100vw" width="934" height="1181"></p><p>The number of proteins that bind with different metals varies dramatically in phytoplankton. For metalloproteins, there are variations in the binding affinities of different metals to organic molecules. For example, the affinities of divalent metals to proteins have a tendency to follow a universal order of preference, namely the Irving-Williams series (Mg<sup>2+</sup> and Ca<sup>2+</sup> [weakest binding] &lt; Mn<sup>2+</sup> &lt; Fe<sup>2+</sup> &lt; Co<sup>2+</sup> &lt; Ni<sup>2+</sup> &lt; Cu<sup>2+</sup> &gt; Zn<sup>2+</sup>). <a href="javascript:void(0);">(43)</a> Cells can simultaneously contain proteins having high-affinity-binding metals, such as Cu and Zn, and others that require uncompetitive metals, such as Mg and Ca. One of the least competitive metals for binding with biological chelators, Mg, is even found to be the most widely distributed metal in the metalloproteomes of various phytoplankton species in this study, supporting the observations of high concentrations in the protein fraction of cells (at millimolar concentrations <a href="javascript:void(0);">(31)</a>).</p><p>In all phytoplankton species in our analysis, the Mg-containing proteins are found to have the highest proportion in all metalloproteins identified. About 30% of the metalloproteins in different strains of phytoplankton are found to contain Mg or depend on it for function (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig2">Figure 2</a>), suggesting the important role of Mg in phytoplankton cells. It is generally coordinated in protein complexes by ATP or ADP and has also been found to be largely distributed in the chloroplasts or thylakoids in <i>G. huxleyi</i> and <i>Synechocystis</i>. <a href="javascript:void(0);">(22,24)</a> The essential roles of Mg in plants have been reported in various studies. Mg<sup>2+</sup> is known to be the central atom of the chlorophyll molecule, and its levels in the chloroplast regulate the activity of key photosynthetic enzymes. <a href="javascript:void(0);">(44)</a> It was reported that approximately 15–35% of total Mg is in the chloroplasts of a plant, <a href="javascript:void(0);">(45)</a> and it also serves as an osmotically active ion in the regulation of cells <a href="javascript:void(0);">(46)</a></p><h4><strong>Comparison Between Metallome Measured Directly and Reconstructed from Proteins</strong></h4><p>A large proportion of metals, such as Fe, Mn, and Cu, are found in membrane proteins,and may contribute significantly to total cellular quotas. <a href="javascript:void(0);">(54,55)</a> However, for method development purpose, we endeavored to remove the cell membranes from our samples to focus mostly on the analysis of intracellular metal quotas and metalloproteins in the cytosol fraction in this study. We note that our analysis includes some membrane-bound proteins likely derived from organelles or surface membranes disrupted during sonication.</p><p>The derivation of cellular metal composition, as determined by two different methods, is compared in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>. The abundance of metal in the proteins is determined as the relative abundance of the protein multiplied by the number of metal ions needed for the specific protein. The abundance of the proteins was normalized to ensure that the total intensities from each replicate measurement for the same species were similar. Since the proteomic data used here were generated from different studies using different instruments, we cannot quantify or compare the absolute biomass of proteins used for each species. Therefore, we cannot compare the absolute trace metal abundance between species solely from metal inferences of expressed proteomes. Nevertheless, we can estimate the relative abundance of different metals in the same species. For the elements that are both measured by the ICP-MS and identified in the proteome, there are generally good correlations (<i>r</i><sup>2</sup> = 0.81 in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>a and <i>r</i><sup>2</sup> = 0.74 in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>b) between the two approaches. This indicates that the metal compositions determined from the protein modeling may be reliable and, therefore, can reflect the metal usage in different phytoplankton. This would provide a level of assurance to further investigate the metal binding associated with various proteins, thereby deepening our understanding of the roles that different metals play in phytoplankton.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Environ_Sci_Technol_2025_59_50_27367_27377_Figure_3_Relationship_between_the_abundance_of_metals_determined_by_ICP_MS_analysis_e7b259e376.jpeg" alt="Environ. Sci. Technol. 2025, 59, 50, 27367–27377: Figure 3. Relationship between the abundance of metals determined by ICP-MS analysis (normalized to C, mmol/mol C) and metal abundance determined from the metalloproteome analysis (the unit is arbitrary). To account for strain variations, the ICP-MS data for the cellular metal content of G. huxleyi are average values from 7 G. huxleyi strains isolated from different areas of the ocean, and the proteome-derived metal composition data are average values calculated from 2 of those G. huxleyi strains (OA1 and OA16) isolated previously, and data from Mckew et al., 2013. (24) The ICP-MS data for Synechocystis are from Synechocystis sp. FACHB898 measured in this study, and the proteome data are calculated from Chen et al., 2018. (22) Noted that the abundance of metals from each proteome was calculated by using the metal stoichiometry multiplied by the relative abundance of proteins, which provides only a relative relationship of different trace metals, not absolute abundance. The symbols represent the mean values, and the error bars indicate standard deviations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Environ_Sci_Technol_2025_59_50_27367_27377_Figure_3_Relationship_between_the_abundance_of_metals_determined_by_ICP_MS_analysis_e7b259e376.jpeg 245w," sizes="100vw" width="1384" height="641"></p><p>Compared to the other metals investigated in this study, Ca and Mg appear to have a much higher relative abundance in the ICP-MS analysis than in the proteomic analysis of <i>G. huxleyi</i> and <i>Synechocystis</i> (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>). These two elements are essential macronutrients for phytoplankton and plants. Ca is the most prominent secondary messenger that plays a crucial role in response to extracellular stimuli in all eukaryotes. Ca<sup>2+</sup> fluxes are transported via Ca channels and transporters, such as Ca-ATPase, regulating a series of important biological functions, including mineral uptake and utilization, K<sup>+</sup> homeostasis adjustment, and regulatory network activity in response to P or N deficiency. <a href="javascript:void(0);">(56)</a> Ca is not always incorporated into protein structures. The Ca abundance determined from the proteins in this study, therefore, is much lower than the actual Ca needed intracellularly. Similarly, the intracellular Mg<sup>2+</sup> and K<sup>+</sup> abundances should also be higher than those determined from the protein structures. Free Mg<sup>2+</sup> and K<sup>+</sup> are known to regulate cation–anion balance and serve as osmotically active ions in the regulation of cells <a href="javascript:void(0);">(46)</a> but free metal ion concentrations cannot be determined through the approach used in this study. Thus, it is not surprising to see much higher relative abundances of Ca and Mg in ICP-MS analysis than in proteomic analysis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>).</p>

Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes

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Thermo Scientific Syncronis Amino HPLC Columns

Improve the consistency and reliability of your liquid chromatography (LC) separations with Thermo Scientific™ Syncronis™ Amino LC Columns.

<p><strong>Improve the consistency and reliability of your liquid chromatography (LC) separations with Thermo Scientific™ Syncronis™ Amino LC Columns. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis and quality control.</strong></p><p>High surface area silica, dense stationary phase coverage and double end-capping minimize secondary interactions and ensure reproducible high-resolution separations.</p><h5><strong>Highly Reproducible Separations</strong></h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li><li>Double endcapped for extra surface coverage</li></ul><h5><strong>Exceptional Column-to-Column Consistency</strong></h5><ul><li>Manufactured, packed and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5><strong>Excellent Performance in Four Modes</strong></h5><ul><li>Aminopropyl phase performs well in reversed phase, normal phase, weak ion exchange and HILIC modes</li></ul><h5><strong>Available in Three Particle Sizes</strong></h5><ul><li>1.7μm for rapid ultrahigh-performance LC (UHPLC) separations</li><li>3μm and 5μm for more traditional HPLC analysis</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Mixed mode</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Max. Pressure:</strong> 5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> Amino (NH2)</li><li><strong>USP Type:</strong> L8</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load:</strong> 4%</li><li><strong>Phase:</strong> Reversed Phase, Normal Phase, Ion Exchange, HILIC</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Syncronis Phenyl  HPLC Columns

Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. 

<p><strong>Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. High surface area silica, dense stationary phase coverage, and double end-capping minimize secondary interactions and deliver reproducible high-resolution separations. The Phenyl-bonded aromatic phase offers alternative selectivity to C18 columns and is particularly useful for optimized retention of aromatic analytes.</strong></p><p>Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.</p><h5><strong>Highly Reproducible Separations</strong></h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li><li>Double endcapped for extra surface coverage</li></ul><h5><strong>Exceptional Column-to-Column Consistency</strong></h5><ul><li>Manufactured, packed and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5><strong>Alternative Selectivity</strong></h5><ul><li>Enhanced retention of aromatic compounds</li></ul><h5><strong>Available in Three Particle Sizes</strong></h5><ul><li>1.7μm for rapid ultrahigh-performance LC (UHPLC) separations</li><li>3μm and 5μm for more traditional HPLC analysis</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Reversed Phase</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Max. Pressure:</strong> 5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> Phenyl</li><li><strong>USP Type:</strong> L11</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load:</strong> 11%</li><li><strong>Phase:</strong> Reversed Phase,</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Syncronis PREP HILIC HPLC Columns

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes.

<p><strong>Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations.&nbsp;</strong></p><p>The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. Our columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Zwitterionic bonded phase enhances retention of polar and hydrophilic analytes.</li><li>No need for ion-pair or derivatization.</li><li>Enhanced sensitivity with MS detection.</li><li>Highly organic mobile phase facilitates desolvation in ESI-MS</li></ul><p><strong>Preparative HPLC Column Hardware Options</strong> - based on column inner diameter (i.d.) and column performance needs.</p><h5><strong>Standard Threaded and Standard Flange Hardware</strong></h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5><strong>XtendedLife Hardware</strong></h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul><p>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> HILIC</li><li><strong>Endcapped:</strong> No</li><li><strong>Max. Pressure:</strong> 2000 psi (140 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> Zwitterionic</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load: </strong>5%</li><li><strong>Phase:</strong> HILIC</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Dionex IonPac AS10 IC Columns

Analyze trace-levels of inorganic anions and organic acids using isocratic or gradient methods with the Thermo Scientific™ Dionex™ IonPac™ AS10 IC Column.

<p><strong>Analyze trace-levels of inorganic anions and organic acids using isocratic or gradient methods with the Thermo Scientific™ Dionex™ IonPac™ AS10 IC Column. Its high capacity permits the analysis of trace-level analytes in high-ionic-strength matrices. Use the Dionex IonPac AS10 column for the analysis of inorganic anions in high nitrate samples.</strong></p><h5><strong>Superior Chromatographic Performance in High Nitrate Samples</strong></h5><ul><li>Excellent resolution of weakly retained low-molecular-weight aliphatic acids</li><li>Selectivity and high capacity for analysis of trace-level analytes in high-ionic-strength matrices</li><li>Strongly retains nitrate for high-resolution analysis of trace anions in nitric acid and nitrate salts</li></ul><h5><strong>For Best Results</strong></h5><ul><li>Use a Dionex eluent generator for simplified eluent preparation for applications using less than 100mN hydroxide</li><li>Use the Thermo Scientific™ Dionex™ Anion Self-Regenerating Suppressor (ASRS™ 300) with the Dionex IonPac AS10 column for continuous suppression of traditional eluents and more concentrated eluents up to 200mM NaOH</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 170 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Separation of Inorganic Anions and Organic Acids</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS10</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li><li><strong>pH: </strong>0 to 14</li></ul>

Thermo Scientific Dionex IonPac AS11 IC Columns

Resolve a large number of inorganic anions and organic acids in approximately 15 minutes with the Thermo Scientific Dionex IonPac AS11 Analytical & Guard Column. 

<p><strong>Resolve a large number of inorganic anions and organic acids in approximately 15 minutes with the Thermo Scientific™ Dionex™ IonPac™ AS11 Analytical &amp; Guard Column. The Dionex IonPac AS11 is a relatively low-capacity column designed for fast gradient screening of inorganic anions and organic acid anions in simple sample matrices such as from foods, beverages, chemical process solutions, wastewaters, brines, and power plant waters.</strong></p><h5><strong>Superior Chromatographic Performance:</strong></h5><ul><li>Facilitates rapid analysis of organic acids and inorganic anions in well-characterized samples</li><li>Ideal for highly charged anions, including polyphosphates and polycarboxylates</li><li>Resolves organic acids and inorganic anions in complex sample matrices</li><li>Shortens analysis time and speeds throughput</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 45 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 45 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Analysis of Inorganic and Organic Acid Anions</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS11</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li><li><strong>pH: </strong>0 to 14</li></ul>

Thermo Scientific Dionex IonPac AS12A IC Columns

Thermo Scientific Dionex IonPac AS12A Analytical and Guard Columns resolve trace chloride and sulfate in high-carbonate samples

<p><strong>Separate inorganic anions and oxyhalides, such as chlorite and bromate, in environmental samples. The Thermo Scientific™ Dionex™ IonPac™ AS12A Analytical and Guard Columns resolve trace chloride and sulfate in high-carbonate samples and meet or exceed the requirements of U.S EPA Methods 300.0 (A) and (B). However, the advanced, high-capacity IonPac AS23 Carbonate Eluent Anion-Exchange column is recommended for determination of oxyhalides and inorganic anions, such as trace bromate, in ozonated drinking water.</strong></p><h5><strong>Fast Isocratic Separation of Inorganic Anions and Oxyhalides in Diverse Matrices</strong></h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A) and (B)</li><li>Separation of common inorganic anions and oxyhalides including chlorite, bromate and chlorate in less than 12 minutes</li><li>Superior retention and quantification of fluoride</li><li>Trace analysis of chloride and sulfate in high-carbonate samples</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5><strong>Multiple Formats Available for Application Flexibility</strong></h5><ul><li>4x200mm, 2x200mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 200mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 52 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 52 μeq</li><li><strong>Column Chemistry:</strong> Carbonate/Bicarbonate</li><li><strong>For Use With (Application):</strong> Separation of Inorganic Anions</li><li><strong>Ion Exchange Group: </strong>Alkyl Quaternary Ammonium</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS12A</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS11-HC IC Columns

Resolve a large number of inorganic anions and organic acid anions in complex matrices with the Thermo Scientific Dionex IonPac AS11-HC Capillary, Analytical & Guard Column.

<p><strong>Resolve a large number of inorganic anions and organic acid anions in complex matrices with the Thermo Scientific™ Dionex™ IonPac™ AS11-HC Capillary, Analytical &amp; Guard Column. Complex sample matrices such as chemical wastewater effluents and fermentation broth solutions contain a variety of inorganic anions and organic acids. With the high-capacity Dionex IonPac AS11-HC column, you can inject higher concentration samples without column overloading or peak broadening.</strong></p><p>The Dionex IonPac AS11-HC column was specifically designed to resolve analytes in a single run using a hydroxide gradient. The high-capacity column delivers improved separation for monovalent carboxylic acids including: quinate, lactate, acetate, propionate, formate, and butyrate.</p><h5><strong>High Capacity for Higher Resolution</strong></h5><ul><li>Requires longer retention times, but with higher resolution</li><li>Facilitates rapid analysis of organic acids and anions in well-characterized samples</li><li>Ideal for organic acids and anions in complex sample matrices or uncharacterized samples</li><li>Recommended for monovalent and divalent organic acids</li></ul><h5><strong>Reduce Operating Costs With Lower Eluent Requirements</strong></h5><p>The new Dionex IonPac AS11-HC Capillary column is packed with the same material as the equivalent standard bore and microbore versions (providing the same performance as a 4mm and 2mm column) using only 1% of the eluent flow rate, which helps reduce operating costs.</p><p>Use the Thermo Scientific™ Dionex™ IonPac™ ICE-AS1 or ICE-AS6 columns for organic acids in high-ionic-strength samples.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 290 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 290 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS11-HC</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS14 IC Columns

Deliver excellent results for fast isocratic separations of inorganic anions in diverse matrices with the Thermo Scientific Dionex IonPac AS14 Carbon Eluent Anion-Exchange Column.

<p><strong>Deliver excellent results for fast isocratic separations of inorganic anions in diverse matrices with the Thermo Scientific™ Dionex™ IonPac™ AS14 Carbon Eluent Anion-Exchange Column. This universal column for the separation of inorganic anions delivers improved peak resolution and retention of fluoride in applications performed previously with the Dionex IonPac AS4A, AS4A-SC and AS12A columns, however, the Dionex IonPac AS22 Carbonate Eluent Anion-Exchange column, an advanced carbonate eluent column, is recommended for fast analysis of inorganic anions.</strong></p><h5><strong>Fast Separation of Inorganic Anions in Diverse Matrices</strong></h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Separation of common inorganic anions in 13 min in drinking water, wastewater, food &amp; beverages, scrubber solutions, and other diverse sample matrices</li><li>Superior retention and quantification of fluoride, glycolate, acetate, and formate</li><li>Compatible with a sodium tetraborate gradient to optimize difficult separations</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5><strong>Multiple Formats Available for Application Flexibility</strong></h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 25mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><p><i><strong>Note: </strong>Use with the Dionex IonPac AS14 Eluent Concentrate for simple, accurate eluent preparation.</i></p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 65 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 65 μeq</li><li><strong>Column Chemistry:</strong> Carbonate/Bicarbonate</li><li><strong>For Use With (Application):</strong> Analysis of Fluorides and Other Inorganic Anions</li><li><strong>Ion Exchange Group: </strong>Alkyl Quaternary Ammonium</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 2 to 12, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS14</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li><li><strong>pH: </strong>2<strong> </strong>to 12</li></ul>

Thermo Scientific Dionex IonPac AS14A IC Columns

Thermo Scientific Dionex IonPac AS14A IC Column is high capacity anion-exchange column for fast isocratic separation of inorganic anions in environmental matrices

<p><strong>Deliver fast isocratic separation of inorganic anions from diverse and complex matrices such as drinking water, wastewater, foods and beverages, and scrubber solutions with the Thermo Scientific™ Dionex™ IonPac™ AS14A Carbon Eluent Anion-Exchange Column. The Dionex IonPac AS14A column meets or exceeds the requirements of U.S EPA Method 300.0 (A). However, the Dionex IonPac AS22 Carbonate Eluent Anion-Exchange column, an advanced carbonate eluent column, is recommended for fast analysis of inorganic anions. For the fastest separation of inorganic anions using a carbonate/bicarbonate eluent, the Dionex IonPac AS22-Fast column is recommended.</strong></p><h5><strong>Fast Separation of Inorganic Anions in Diverse Matrices</strong></h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Separation of common inorganic anions in 8 min (3 x 150mm format) or 13 min (4 x 250mm format) in diverse sample matrices</li><li>Superior retention and quantification of fluoride</li><li>Compatible with sodium or potassium hydroxide gradient to optimize difficult separations</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5><strong>Multiple Formats Available for Application Flexibility</strong></h5><ul><li>4x250mm, 3x150mm analytical columns</li><li>4x50mm, 3x30mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Shorter 150mm columns produce faster runs of relatively clean matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><p><i><strong>Note: </strong>Use with the Dionex IonPac AS14 Eluent Concentrate and the Combined Seven Anion Standard for simple, accurate eluent preparation.</i></p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 65 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 120 μeq</li><li><strong>Column Chemistry:</strong> Carbonate/Bicarbonate</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkyl Quaternary Ammonium</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 2 to 11, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS14A</li><li><strong>Format:</strong> Standard Bore</li><li><strong>pH: </strong>2<strong> </strong>to 11</li></ul>

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