<h3><strong>How are PFAS regulations changing, and what role will ion chromatography play in the future of environmental testing?</strong></h3><p>In this episode, &nbsp;David interviews <strong>Jay Gandhi, PhD, </strong>Vertical Markets Manager at <strong>Metrohm USA</strong>, for an in-depth discussion on PFAS analysis, the rise of AOF (Adsorbable Organic Fluorine) testing, and the development of EPA 1621 and ISO 18127 standards.</p><p>With more than 30 years of experience in ion chromatography, Jay shares valuable perspective on how the field has evolved—from early applications and NASA water recovery projects to today’s challenges involving PFAS, TFA, and emerging contaminants.</p><h4><strong>Topics covered in this episode include:</strong></h4><ul><li>What PFAS are and why they matter</li><li>The difference between targeted PFAS methods and AOF analysis</li><li>How combustion ion chromatography works</li><li>Behind the scenes of EPA 1621 and ISO 18127 method development</li><li>Regulatory trends in water, textiles, and food packaging</li><li>TFA analysis using IC-MS and IC-MS/MS</li><li>The future of ion chromatography in environmental labs</li></ul><h3><strong>Video Transcription</strong></h3><p>For more than three decades, Jay Gandhi has worked with ion chromatography (IC), watching the technique expand from a specialized tool for water analysis into an analytical platform used across environmental testing, food and beverage analysis, semiconductor manufacturing, battery applications, and, increasingly, PFAS analysis.</p><p>In an interview for <i>Concentrating on Chromatography</i>, Gandhi discusses his introduction to IC during his work on a NASA Mars exploration project, the emergence of PFAS as a major analytical challenge, and why techniques such as adsorbable organic fluorine (AOF) determination and combustion ion chromatography (CIC) can provide information that targeted LC-MS analysis alone cannot deliver. He also examines the development of standardized methods, sample preparation challenges, and the future role of IC-MS and total fluorine approaches in PFAS monitoring.</p><h4><strong>From NASA to ion chromatography</strong></h4><p>Gandhi's introduction to ion chromatography came through his work as a principal scientist on a NASA Mars exploration project focused on water recovery under zero-gravity conditions. IC played an important role because it enabled researchers to establish the ionic balance of anions and cations in water.</p><p>The technology was used both to investigate micronutrients relevant to agricultural applications and to evaluate water purity for potential astronaut consumption. During approximately three years of this work, Gandhi gained extensive experience with ion chromatography. This ultimately led him deeper into IC instrumentation and, 24 years ago, to joining Metrohm.</p><p>At the beginning of his career, IC was strongly associated with relatively straightforward anion and cation analysis in water. The technique itself had been used for water analysis since the 1970s, but Gandhi has seen its application range expand dramatically.</p><p>Food and beverage testing and environmental analysis became important fields, supported by both direct and indirect detection strategies. More recently, IC has also found applications in areas such as the semiconductor industry and battery recycling.</p><p>In Gandhi's words, ion chromatography has effectively "bloomed" into numerous application areas.</p><h4><strong>PFAS changes the analytical challenge</strong></h4><p>PFAS represent a particularly important chapter in this development. These chemicals have been used for decades in numerous consumer and industrial products, including non-stick materials, firefighting foams, water-resistant textiles and other applications.</p><p>Gandhi recalls work on PFOA and PFOS using ion chromatography with conductivity detection as early as 2008. However, the achievable detection limits were insufficient for the extremely low concentrations that later became important in PFAS analysis.</p><p>A major turning point for him came in 2015, when he encountered research from Sweden using <strong>combustion ion chromatography to investigate total fluorine</strong>. Subsequent collaboration with researchers at Clarkson University and Harvard University, particularly on aqueous film-forming foam (AFFF), further demonstrated the importance of looking beyond individual targeted PFAS compounds.</p><h4><strong>Why targeted LC-MS does not tell the whole story</strong></h4><p>Targeted LC-MS is highly sensitive and selective and can provide detailed information about individual PFAS compounds. However, according to Gandhi, the number of possible PFAS structures in the environment is vastly greater than the number routinely included in targeted methods.</p><p>A targeted method may monitor dozens or perhaps around one hundred compounds, while the overall PFAS universe can include thousands of chemical structures. Consequently, targeted analysis may characterize only a small fraction of the total PFAS burden.</p><p>There are other practical considerations. LC-MS measurements can be affected by matrix-dependent ion suppression, while accurate quantification frequently relies on isotope-labelled internal standards. Such standards are available for only a limited number of compounds and can be expensive.</p><p>This is where measurements of total or adsorbable organic fluorine can provide complementary information. Rather than asking <i>which specific PFAS compounds are present?</i>, these approaches can help answer a broader question: <i>how much fluorine associated with this chemical space is present in the sample?</i></p><h4><strong>AOF as a complementary approach</strong></h4><p>Adsorbable organic fluorine analysis provides a different perspective from targeted LC-MS. In the workflow discussed by Gandhi, organic fluorine compounds are captured during sample preparation and subsequently measured using combustion ion chromatography.</p><p>The principle of CIC builds upon older total organic halogen analysis. The sample is combusted at temperatures above approximately 1,000 °C, breaking carbon-halogen bonds. In PFAS-related analysis, carbon-fluorine bonds are broken and the resulting fluoride is collected in an absorption solution.</p><p>Ion chromatography is then used to separate and quantify the fluoride.</p><p>Replacing older detection approaches with IC provides both sensitivity and selectivity for the halogens being measured. Effective combustion is critical: oxygen supports the combustion process, while a carrier gas transports the products into the absorption solution for subsequent IC analysis.</p><h4><strong>The challenge of inorganic fluoride</strong></h4><p>The broad response of CIC is simultaneously one of its strengths and one of its analytical challenges. Combustion does not distinguish fluorine originating from PFAS from fluorine present in inorganic compounds such as fluoride salts.</p><p>This becomes particularly important when analyzing water containing relatively high concentrations of inorganic fluoride while attempting to quantify organic fluorine at very low levels.</p><p>Sample preparation therefore has a critical role. Inorganic fluoride must be effectively separated from the fraction of interest, while materials used during sample preparation must themselves have sufficiently low fluoride backgrounds.</p><p>Contamination can come from unexpected sources. Gandhi points out that background fluoride may originate not only from reagents and sample preparation materials but potentially from the laboratory environment. Careful blank control, appropriate materials and rigorous evaluation of interferences are therefore essential when moving toward increasingly low detection limits.</p><h4><strong>From research method to international standardization</strong></h4><p>The development of standardized AOF methods illustrates the importance of collaboration between academia, industry, regulatory organizations and analytical laboratories.</p><p>Gandhi describes work that began in Europe around 2015 and subsequently progressed through German standardization activities, ISO development, ASTM-related collaboration and the U.S. EPA. The process eventually contributed to methods such as <strong>EPA Method 1621</strong>.</p><p>He emphasizes that this was not the work of a single scientist or organization. Developing a robust method required years of experiments, discussions and sometimes difficult technical debates among numerous stakeholders.</p><p>For Gandhi, the essential principle was that experimental evidence ultimately had to determine the outcome. Collaboration becomes possible when scientists focus on the data rather than the organizations they represent.</p><h4><strong>PFAS monitoring is moving beyond drinking water</strong></h4><p>Regulatory attention to PFAS is also expanding beyond drinking water. Gandhi describes a broader strategy that begins with reducing the manufacture and use of PFAS-containing materials and continues with removing existing contamination from the environment.</p><p>Wastewater represents an important part of this picture because PFAS can enter it from numerous sources. Historical contamination from landfills and contaminated sites can also migrate into groundwater and ultimately affect drinking-water supplies.</p><p>Consumer products are another area of increasing attention. Food packaging, disposable containers, automotive fabrics, outdoor clothing and water-repellent textiles have historically used fluorinated substances to provide properties such as grease, water or stain resistance.</p><p>Reducing PFAS use in such products could therefore gradually decrease the amount entering waste streams and the wider environment.</p><h4><strong>Screening first, regulation later?</strong></h4><p>Gandhi expects AOF to be particularly valuable as a <strong>screening tool</strong>, providing an estimate of broader fluorine contamination before laboratories proceed to more specific analyses.</p><p>Its importance becomes clear when AOF results are compared with targeted PFAS measurements. Gandhi reports that laboratories working with EPA Method 1621 have observed differences between targeted PFAS results and broader AOF measurements ranging from approximately <strong>300- to 4,000-fold in the total fluorine mass balance</strong>.</p><p>Such discrepancies suggest that targeted methods can leave a substantial portion of fluorine unexplained.</p><p>Rather than competing with LC-MS, AOF and CIC therefore provide complementary information. A broad screening method can reveal the magnitude of total contamination, while targeted mass spectrometry identifies and quantifies specific compounds of interest.</p><h4><strong>Where IC-MS fits into the PFAS toolbox</strong></h4><p>No single technique can cover the entire PFAS problem. Gandhi highlights very short-chain, highly polar fluorinated compounds as another analytical challenge.</p><p>Compounds such as trifluoroacetic acid (TFA) are highly water-soluble and may not be quantitatively retained by the carbon materials used for AOF sample preparation. Direct ion chromatography can provide an alternative route for separating such compounds from common inorganic anions.</p><p>Coupling IC with mass spectrometry adds another level of sensitivity and selectivity. Gandhi therefore sees <strong>IC-MS and IC-MS/MS</strong> as increasingly important tools for highly polar fluorinated compounds. Similar approaches can also be useful beyond PFAS, including the determination of polar pesticides such as glyphosate and glufosinate.</p><h4><strong>Toward lower detection limits and more complete PFAS analysis</strong></h4><p>For Gandhi, current standardized methods represent a beginning rather than an endpoint. Improvements in instrumentation and particularly sample preparation could push total fluorine analysis to substantially lower concentrations.</p><p>He notes that current combustion IC configurations can reach approximately <strong>0.5 ppb</strong>, or 500 parts per trillion, and believes that improvements in sample volume, preparation procedures and mitigation of inorganic fluoride backgrounds could eventually move detection below 100 ppt and potentially toward 50 ppt.</p><p>Extractable organic fluorine workflows could provide another bridge between broad fluorine screening and targeted LC-MS methods. Combining improved extraction with CIC could allow samples to be screened for organic fluorine before more detailed compound-specific characterization.</p><p>The broader message from Gandhi's career is that PFAS analysis is unlikely to be solved by a single analytical technique. <strong>Targeted LC-MS provides compound-specific sensitivity and selectivity; AOF and combustion IC reveal a broader fluorine picture; and IC-MS can address highly polar compounds that are difficult to capture using conventional workflows.</strong> Used together, these techniques can provide laboratories with a much more complete understanding of PFAS contamination than any one approach alone.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

<p><strong>In late February 2025, a train carrying benzene derailed in Hustopeče nad Bečvou, and within hours, a race was on to save the groundwater and surface water. One of the most toxic industrial substances began seeping into the gravel-sand subsoil. While fieldwork was underway to prevent the spread of contamination, decisions were already being made in the CXI TUL laboratories regarding how to subsequently clean up the contaminated rock environment.</strong></p><p>The accident ranks among the <strong>most serious environmental incidents in the Czech Republic in recent years</strong>. After a tanker train derailed, hundreds of<strong> metric tons of benzene leaked</strong>; due to the highly permeable geological subsoil, it quickly seeped into deeper soil layers and into the groundwater. The combination of the substance’s high toxicity and local conditions complicated both the emergency response and the subsequent remediation. The key was to stop further spread, determine the extent of the contamination, and establish a remediation strategy for the entire site.</p><p>It was at this stage that CXI TUL became involved in the solution. At the request of the renowned remediation company DEKONTA, a.s., a team led by <strong>RNDr. Jan Němeček, Ph.D.</strong>, began testing the feasibility of the<strong> in situ chemical oxidation (ISCO) method</strong>. This technology is designed to<strong> break down benzene directly within the rock matrix</strong>, without the need for extensive excavation of contaminated soil or the pumping out of large volumes of groundwater. The goal was simple: to find a method that would work quickly, safely, and under the actual conditions of the affected site.</p><h4><strong>Benzene Remediation: Technically Simple, Complicated in Practice</strong></h4><p>Benzene is a highly toxic and carcinogenic substance that rapidly penetrates into groundwater in permeable subsoil, where it dissolves and spreads further as the water flows toward surface water bodies. Benzene also binds to rock particles and migrates in the form of vapors into overlying unconsolidated layers.</p><p>Scientists in Liberec therefore tested <i>in situ </i>chemical oxidation (ISCO) technology under laboratory conditions. The goal was to find an oxidant capable of breaking down benzene directly underground into less hazardous substances.</p><p>The tests evaluated two main options:<strong> activated hydrogen peroxide and sodium peroxodisulfate</strong>. Both substances removed up to<strong> 99.99% of the benzene</strong>, but there was a difference in their kinetics and impacts on the natural environment. Activated hydrogen peroxide achieved nearly complete degradation of the contaminant within a single day. Activated sodium peroxodisulfate was slower and also significantly altered the chemistry of the water; it acidified the environment and increased the sulfate content in the water, which poses an additional environmental burden.</p><p>For field applications, the researchers therefore unequivocally recommended <strong>activated hydrogen peroxide</strong>.</p><h4><strong>Dosage Matters</strong></h4><p>It was not just the oxidant’s effectiveness that was key. It was equally important to determine how the chemical reaction would behave in a real geological environment. The team of scientists therefore also analyzed what is known as <strong>soil oxidant consumption (SOD)</strong>. It turned out that a significant portion of the oxidizing agent does not react solely with benzene but is also consumed to a significant extent by the rock environment itself, particularly by natural organic substances present in the fine-grained fraction of the soil.</p><p>An oxidant consumption value was determined specifically for the local composition of the aquifers, which is essential for the correct dosing of chemicals in the field so that the remediation is not only effective but also economically viable and safe for surrounding ecosystems.</p><h4><strong>One Year After the Accident: Research Turns into Real-World Assistance</strong></h4><p>While the public often perceives university science as a distant world of laboratories and grant projects, it is precisely situations like this that demonstrate its practical impact. The results of the work by researchers at CXI TUL did not remain confined to academic studies but directly influenced decisions regarding the technology used to clean up one of the largest environmental accidents in recent years.</p>

Science on the Front Lines: Cleanup of a Benzene Spill

<p><strong>This study uses ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) lipidomics to characterize plasma lipid alterations associated with long-term aromatase inhibitor therapy in postmenopausal breast cancer patients. A total of 649 lipid species across 23 classes and subclasses were relatively quantified, revealing significant differences in phosphatidylcholines, sphingomyelins, ceramides, hexosylceramides, and other lipid groups.</strong></p><p>Multivariate analysis showed clear group separation, while a Naive Bayes model based on lipid-class profiles achieved an AUC of 0.79. The results indicate that aromatase inhibitor therapy is associated with systematic lipidomic changes linked to estrogen deprivation and sphingolipid metabolism, providing potential mechanistic insight into long-term metabolic effects of treatment.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926"><strong>Aromatase Inhibitor Therapy Is Associated with Distinct Plasma Lipidomic Profiles in Postmenopausal Breast Cancer Patients&nbsp;</strong></a></h4><div><span>Aleksandra Arsic, Ales Kvasnicka</span><a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0001-5973-316X"><span><img></span></a><span>, David Friedecky</span><a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0002-3448-9073"><span><img></span></a><span>, Nebojsa Ivanovic, Maja Milosevic</span><a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0002-0985-0728"><span><img></span></a><span> and Vesna Vucic</span></div><p>Int. J. Mol. Sci. <strong>2026</strong>, 27(4), 1926</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.3390/ijms27041926">https://doi.org/10.3390/ijms27041926</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><div><p>Breast cancer (BRC) is the most common malignancy among women, with over 2.2 million new cases diagnosed globally in 2022 [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B1-ijms-27-01926">1</a>]. A substantial proportion of breast cancers are estrogen receptor-positive (ER+) and/or progesterone receptor-positive (PR+) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B2-ijms-27-01926">2</a>], necessitating multimodal treatment approaches, including surgery, chemotherapy, radiotherapy, and endocrine therapy. Aromatase inhibitors (AIs) represent the gold-standard adjuvant endocrine treatment for postmenopausal women, as they improve disease control and reduce the risk of distant metastasis by inhibiting peripheral aromatase activity [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B3-ijms-27-01926">3</a>]. This inhibition prevents the conversion of androgenic precursors into estrogens in peripheral tissues, resulting in a marked reduction in systemic estrogen levels. For postmenopausal women with ER+ and PR+ breast tumors, a five-year course of AI therapy, often extended beyond this duration, is recommended to reduce the risk of disease recurrence [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B4-ijms-27-01926">4</a>].</p><p>AIs decrease plasma and tissue concentrations of endogenous estrogens by inhibiting aromatase (cytochrome P450), the rate-limiting enzyme responsible for estrogen biosynthesis from testosterone and adrenal androgens [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B5-ijms-27-01926">5</a>]. Because estrogen plays a protective role in lipid metabolism, AI therapy is frequently associated with dyslipidemia. Increased levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL) have been reported in patients receiving AIs [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B6-ijms-27-01926">6</a>], thereby increasing the risk of cardiovascular disease (CVD) in postmenopausal women with BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B7-ijms-27-01926">7</a>].</p><p>Disturbances in lipid metabolism are recognized as a hallmark of cancer, leading to increasing interest in lipidomic profiling as a tool for characterizing tumor-associated metabolic alterations [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B8-ijms-27-01926">8</a>]. In recent years, clinical lipidomics studies have been conducted across multiple malignancies, including lung [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B9-ijms-27-01926">9</a>], pancreatic [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B10-ijms-27-01926">10</a>], renal cell carcinoma [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B11-ijms-27-01926">11</a>], colorectal [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B12-ijms-27-01926">12</a>], and BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B13-ijms-27-01926">13</a>], highlighting cancer-specific lipidomic signatures and the growing relevance of lipid metabolism in oncology. Lipidomic reprogramming is now recognized as a defining feature of BRC. To date, elevated levels of phosphatidylcholine (PC 32:1), stearic acid, and ceramide (Cer 43:1), along with reduced levels of diacylglycerol (DG 34:2), have been reported in BRC patients compared with healthy women [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B14-ijms-27-01926">14</a>]. These lipid species, together with phosphatidylinositol (PI 16:0/16:1) and PI (18:0/20:4), have been proposed as potential biomarkers for BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B14-ijms-27-01926">14</a>].</p><p>Despite these advances, there remains a paucity of studies investigating the lipidomic profiles in BRC patients in relation to endocrine therapy. The present cross-sectional study aims to address this gap by conducting mass spectrometry-based profiling of lipid classes and comparing profiles between postmenopausal BRC patients receiving AI therapy and another cohort assessed before this treatment. This approach aims to characterize associations between AI therapy status and plasma lipidomic patterns.</p><h4><strong>2. Results</strong></h4><h5><strong>2.3. Lipidomic Analysis</strong></h5><p>Plasma lipid profiling identified 649 lipids across 23 lipid classes and subclasses, covering the major structural families of the circulating lipidome, including neutral lipids (triacylglycerols, diacylglycerols, cholesteryl esters, and cholesterol), glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylglycerols, phosphatidylserines, and their lysophospholipid counterparts), sphingolipids (sphingomyelins, ceramides, monohexosylceramides, and dihexosylceramides), and free fatty acids. In addition to diacyl phospholipids, the dataset included ether-linked and plasmalogen species (denoted as O- and P-), most prominently within PC, PE, and LPC and LPE classes. An overview of the plasma lipidome is shown as a global lipid network (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f001">Figure 1</a>). To assess whether the observed lipid changes reflect coordinated remodeling rather than isolated signals, we performed Fisher’s combined probability test at the lipid-class level. This analysis revealed significant cumulative differences in several lipid classes, including Cer, LPC, ether/plasmalogen LPC, LPE, and SM (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f001">Figure 1</a>), supporting systematic class-level alterations despite limited power at the single-species level.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Int_J_Mol_Sci_2026_27_4_1926_Figure_1_Global_lipid_network_analysis_of_plasma_lipidomic_differences_between_breast_cancer_patients_BRC_prior_to_initiation_of_the_aromatase_inhibitor_6f82827773.jpg" alt="Int. J. Mol. Sci. 2026, 27(4), 1926: Figure 1. Global lipid network analysis of plasma lipidomic differences between breast cancer patients (BRC) prior to initiation of the aromatase inhibitor (AI) therapy and patients receiving AI. Cytoscape-based network visualization illustrates lipid species grouped according to structural similarity and lipid class. Each node represents an individual lipid species, while edges indicate class-based relationships. Node size corresponds to the −log10 p-value from the Mann–Whitney U test, reflecting the statistical significance (−log p-Value &gt; 1.3) of differences between groups. Node color indicates the direction and magnitude of change, based on log2 fold change, with red representing higher abundance in AI-treated patients and blue representing higher abundance in patients before AI therapy. Major lipid classes, including glycerophospholipids (GPL), sphingolipids (SP), glycerolipids (GL), fatty acids (FA), and cholesteryl esters (CE), are shown to highlight coordinated class- and species-level lipidomic alterations. Fisher’s combined p-value for individual lipid classes is shown at the bottom (significant p-Value &lt; 0.05). " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Int_J_Mol_Sci_2026_27_4_1926_Figure_1_Global_lipid_network_analysis_of_plasma_lipidomic_differences_between_breast_cancer_patients_BRC_prior_to_initiation_of_the_aromatase_inhibitor_6f82827773.jpg 245w," sizes="100vw" width="1600" height="847"></p><h5><strong>2.4. Multivariate Analysis of Plasma Lipidomic Profiles</strong></h5><p>Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were applied to the lipidomic dataset to assess global variation and group separation between the BRC and AI groups (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f002">Figure 2</a>). PCA was performed as an unsupervised analysis, including all samples and quality control (QC) samples. The first two principal components explained 37.9% (PC1) and 15.6% (PC2) of the total variance, accounting for 53.5% of the cumulative variance. QC samples clustered tightly near the center of the PCA score plot, indicating analytical stability and reproducibility of the LC–MS measurements. In the PCA score plot, samples from the BRC and AI groups showed partial overlap, with no complete separation observed along the first two principal components. To further evaluate group discrimination, a supervised OPLS-DA model was constructed using the same dataset. The OPLS-DA score plot demonstrated improved separation between the BRC and AI groups along the first latent variable, with group-specific clustering highlighted by confidence ellipses. Permutation testing confirmed that the supervised OPLS-DA model was not overfitted, with a negative Q<sup>2</sup> intercept (Q<sup>2</sup> = −0.55), indicating that permuted models lacked predictive ability compared with the original model (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Figure S1</a>). Together, these multivariate analyses indicate differences in lipidomic profiles between the BRC and AI groups, while also demonstrating acceptable analytical performance as evidenced by the clustering of QC samples.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Int_J_Mol_Sci_2026_27_4_1926_Figure_2_Multivariate_analysis_of_plasma_lipidomic_profiles_in_breast_cancer_patients_BRC_56f65f265b.jpg" alt="Int. J. Mol. Sci. 2026, 27(4), 1926: Figure 2. Multivariate analysis of plasma lipidomic profiles in breast cancer patients (BRC) prior to initiation of the aromatase inhibitor (AI) therapy and patients receiving AI. Principal component analysis (PCA) score plot (a) shows the distribution of plasma lipidomic profiles from patients before AI therapy (BRC, blue), patients receiving AI therapy (AI, red), and quality control samples (QC, gray). Orthogonal partial least squares–discriminant analysis (OPLS-DA) score plot (b) demonstrates improved separation between BRC and AI groups. The ellipse represents the 95% Hotelling’s T2 confidence interval. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Int_J_Mol_Sci_2026_27_4_1926_Figure_2_Multivariate_analysis_of_plasma_lipidomic_profiles_in_breast_cancer_patients_BRC_56f65f265b.jpg 245w," sizes="100vw" width="1600" height="652"></p><h4><strong>4. Materials and Methods</strong></h4><h5><strong>4.5. Lipidomic Analysis</strong></h5><p>Chromatographic separation was performed using an ultra-high-performance liquid chromatography (UHPLC) <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=ExionLC"><strong>ExionLC system</strong></a> (SCIEX, Framingham, MA, USA) coupled to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=6500"><strong>QTRAP<sup>®</sup> 6500+ mass spectrometer </strong></a>(SCIEX, Framingham, MA, USA). Lipids were separated on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1953"><strong>ACQUITY Premier BEH C8 column</strong></a> (100 × 2.1 mm, 1.7 μm; Waters Corporation, Wilmslow, UK). The column temperature was kept at 55 °C, and the autosampler was set to 10 °C. The mobile phases consisted of solvent A (acetonitrile/water, 6:4, <span>v</span>/<span>v</span>, containing 10 mM ammonium acetate) and solvent B (isopropanol/acetonitrile, 9:1, <span>v</span>/<span>v</span>, containing 10 mM ammonium acetate), delivered at a flow rate of 0.4 mL/min. The total analytical run time was 20 min per sample. Data acquisition was carried out using Analyst software (version 1.6.2), and data processing and semiautomatic peak integration were performed with MultiQuant software (version 3.0; SCIEX, Framingham, MA, USA). Samples were analyzed in a randomized sequence with continuous monitoring of quality control samples. Raw peak areas were QC-normalized by LOESS [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B48-ijms-27-01926">48</a>] and IS-normalized by the corresponding IS class for each lipid.</p><h5><strong>4.6. Statistical Analysis</strong></h5><p>The data were processed using the Metabol package [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B48-ijms-27-01926">48</a>] in R program (v 3.6.3). Preprocessing of the data included locally estimated scatterplot smoothing (LOESS), Pareto scaling, filtering of analytes (coefficients of variation in the QC above 30%), mean centering, and the lnPQN normalization (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Table S1</a>) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B49-ijms-27-01926">49</a>]. Univariate statistical analysis (based on the non-parametric non-paired Mann–Whitney U test (<span>p</span>-values shown as −log) together with the log2 fold-change in medians) was performed and visualized using GraphPad Prism (version 9.0, GraphPad Software, LLC, Boston, MA, USA). Data were also evaluated using multivariate statistical analysis, namely the principal component analysis (PCA) and orthogonal discriminant analysis by partial least squares (OPLS-DA) and validated by permutation using SIMCA software (version 18.0, Sartorius Umetrics, Umeå, Sweden). Benjamini–Hochberg correction was used for false discovery rate correction. Detected lipid species were mapped to standardized lipid classes and subclasses according to accepted lipidomics nomenclature, enabling consistent grouping of lipid species for downstream statistical, class-level, and network analyses. LipidOne 2.4 was further used to support lipid-centric data integration and visualization prior to multivariate and pathway-based analyses (such as heatmaps, ROC analyses using a Naive-Bayesian model and Logistic Regression model, and protein–protein interaction network based on lipidomics data) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B50-ijms-27-01926">50</a>]. Lipid network visualization was performed using Cytoscape software (v 3.8.2) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B51-ijms-27-01926">51</a>]. Differentially abundant lipid species were organized according to lipid class and structural similarity. Node size reflected the −log10 <span>p</span>-value from univariate analysis, while node color represented log2 fold change between groups. Calculation of Fisher’s combined probability test for assessing systematic lipid class-level differences was performed (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Table S2</a>) and calculated following procedures described previously [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B52-ijms-27-01926">52</a>].</p><h4><strong>5. Conclusions</strong></h4><p>In conclusion, our findings demonstrate significant alterations in lipid metabolism in BRC patients undergoing AI therapy, with pronounced changes in Cer, SM, DG, TG, PC, PI, and PE lipids and lipid classes. However, the precise mechanisms underlying the differences in lipid metabolism between patients before and during AI treatment remain to be fully elucidated. Future longitudinal studies with paired pre- and post-treatment samples from the same individuals leveraging comprehensive lipidomic profiling are needed to deepen our understanding of AI-induced metabolic alterations and to inform the development of targeted interventions aimed at mitigating adverse effects, ultimately improving long-term outcomes and quality of life for these patients.</p></div></div>

Aromatase Inhibitor Therapy Is Associated with Distinct Plasma Lipidomic Profiles in Postmenopausal Breast Cancer Patients

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Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer

Perform both basic and complex analyses with the Thermo Scientific™ GENESYS™ 30 Visible Spectrophotometer, an intuitive, reliable solution for education or in the lab.

<h3><strong>Designed for Users, Designed for Results</strong></h3><p><strong>The Thermo Scientific ™ GENESYS ™ 30 is the most thoughtfully designed spectrophotometer available today. Its high resolution color display, large sample compartment, and data export options meet modern expectations for students, educators and lab technicians. The GENESYS 30 blends the simplicity to satisfy novice students with the capabilities that labs need for accurate quantitative analysis.</strong></p><h4><strong>System Benefits:</strong></h4><ul><li>Large sample compartment with front access simplifies sample handling</li><li>High resolution color display</li><li>Outstanding durability in challenging environments thanks to:</li><li>Waterproof screen</li><li>Sealed, rubberized keypad</li><li>Removable, washable sample compartment</li><li>Sloping surfaces that shed spills</li><li>Chemical resistant PC-ABS covers</li><li>3A linear measurement range for concentrated solutions</li><li>Multi-point quant offers higher measurement confidence</li><li>Traditional monochromator paired with advanced electronics delivers outstanding stability</li><li>Scan mode for spectral analysis and interpretation</li><li>Data any way you want it - excellent snap-on thermal printer or USB data export</li><li>Expanded capabilities with optional VISIONlite software</li><li>2-year warranty</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg 195w," sizes="100vw" width="2103" height="1685"></p><h3><strong>Modern Power meets Traditional Simplicity</strong></h3><h4><strong>Live Display and Concentration Mode</strong></h4><p>Replicate the familiar display of absorbance or transmittance, and single standard concentration measurements of prior generation instruments in the GENESYS 30 Live Display mode. All your parameters are set and kept in view on a single screen as the calculated concentration updates every two seconds.</p><h4><strong>Fixed Mode</strong></h4><p>Apply a factor to an absorbance measurement at one wavelength, or measure at two wavelengths with two factors and perform addition, subtraction or division of the values. Display results for up ten samples then print, save or clear your data before going on to the next sample series.</p><h4><strong>Quantitative Analysis with up to Six Standards</strong></h4><p>Go beyond the basic spectrophotometer by recording and storing up to six standards to calibrate your method. Choose a linear fit with an intercept or forced through zero. Name and save your method on the instrument or back it up to a USB memory device. All data are recorded with an index number (S1 – S10) and a time stamp. The result table accommodates up to ten samples for you to print or save before going on to the next sample series or analysis.</p><h4><strong>Go negative!</strong></h4><p>Some methods call for blanking the instrument on a dark standard and reading absorbance in the negative range. Most single beam instruments cannot measure below -0.3A. GENESYS 30 uses advanced signal processing to deliver stable, reliable results down to -3A.</p><h4><strong>Simple to Sophisticated:</strong></h4><p>The GENESYS 30 has the flexibility to perform routine analysis one minute and complex analytical methods the next. The custom Analyzer mode enables you to build your formula with up to six wavelengths, six factors, standard arithmetic operators and absorbance values raised to the 2nd or 3rd power. Use the included Windows® application to design your method. Transfer the method to your GENESYS 30 and run it with the touch of a button. Analyzer mode shows your method name and a continuous display of your results. Technicians can write the result down or print it with a time stamp using the printer accessory.</p><h4><strong>Smarter by Design:&nbsp;</strong></h4><p>GENESYS 30 offers the convenient SmartStart™ feature that has delighted GENESYS 10S users since its introduction. The SmartStart menu gives you direct access to your<strong> 12 favorite methods</strong>. Allowing you to work smarter, not harder.</p><h4><strong>Snap on Printer:&nbsp;</strong></h4><p>Fast and clear printing when you need it. Add printing capability to your instrument. The snap-on thermal printer installs<br>in moments.&nbsp;</p><h4><strong>Quick and Simple Lamp Replacement:&nbsp;</strong></h4><p>No need to remove the cover, just loosen two screws, slide out the old lamp fitting and slide a new one back in. Everything is pre-aligned, just let the new lamp warm up and go back to work.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: Quick and simple lamp replacement." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg 197w," sizes="100vw" width="1737" height="1376"></p><h3><strong>At Home in the Instructional Laboratory</strong></h3><p><strong>GENESYS spectrophotometers are core teaching instruments in chemistry and biology laboratories around the world. The Thermo Scientific™ GENESYS™ 30 allows students and teachers to focus on examining scientific data and learning scientific concepts instead of learning instrumentation.</strong></p><h4><strong>Easy to Teach, Easy to Learn&nbsp;</strong></h4><p>Clearly labeled, uniquely colored keys simplify instrument operation. New users require little or no training.</p><h4><strong>Get Working – Faster!</strong></h4><p>Large, color digital screen and numeric keypad help you set up methods faster. Simplified software interface gives users maximum control and efficiency.</p><h4><strong>Designed for Today, Built for Tomorrow – Inside and Out!</strong></h4><p>On the inside, GENESYS 30 uses advanced optics, electronics and signal processing to deliver superior measurement performance for its class. On the outside, GENESYS 30 presents a tough exterior built to last for years in the most demanding environments.</p><ul><li>Resistant to spills</li><li>Removable and washable sample compartment</li><li>The sample compartment lid<ul><li>stays where you put it - open or closed</li><li>has a magnetic latch to hold it closed during measurements</li><li>is customer-replaceable should it get broken through abuse</li></ul></li></ul><h4><strong>Simple and Familiar</strong></h4><p>Open the Live Display application with a single button-press to monitor the absorbance or transmittance live – just like a GENESYS 20 or SPECTRONIC 20. The spectrum indicator shows the color of light being absorbed.</p><h4><strong>Special support for OD 600 and test-tube measurements</strong></h4><p>For measurements in test-tubes, the test-tube holder accessory accommodates 100mm x 13mm test-tubes with the lid closed. Or add the tall test-tube adaptor to use larger diameter, taller tubes (up to 150mm, 6 inches) with the lid open. Special OD600 mode for cell growth measurements. Select it from the Home menu and go! No method setup required.</p><h4><strong>Scanning: Simple and Fast</strong></h4><p>Find peaks in the spectrum of your sample by scanning across the spectrum in any range from 325nm to 1100nm with 1nm precision and noise-free peaks up to 3A. Use the cursor and zoom controls to identify λ<sub>max</sub>.</p><h4><strong>Data you can use</strong></h4><ul><li>Print your scan and stick it into a lab notebook using the printer accessory and optional self-stick printer paper.</li><li>Save the data points of your scan to a USB memory device and load the data into a spreadsheet or graphing program for optimal reporting.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: USB stick." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg 181w," sizes="100vw" width="543" height="469"></p><h3><strong>VISIONlite Software</strong></h3><p><strong>For users who need the power of computerized data analysis, optional Thermo Scientific™ VISIONlite™ software pairs a simple user</strong><br><strong>interface with a wider range of options for experiment design and data analysis.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific - Genesys spectrophotometers: VISIONlite Software." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h4><strong>Scan Application:</strong></h4><ul><li>Automated peak pick and label</li><li>Overlay spectra and print the results</li></ul><h4><strong>Kinetics Application:</strong></h4><ul><li>Data intervals as low as 1 second</li><li>Enzyme rate calculation with factor</li></ul><h4><strong>Quantitative Analysis:</strong></h4><ul><li>Up to 100 standards</li><li>Linear, quadratic or cubic data fits</li></ul><h4><strong>Performance Verification:</strong></h4><ul><li>Full suite of tests to verify performance of your instrument</li><li>Pre-loaded pass/fail limits specific to your instrument</li></ul><h3><strong>GENESYS 30 Accessories</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: GENESYS 30 Accessories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg 243w," sizes="100vw" width="1444" height="929"></p><h3><strong>GENESYS 30 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> &lt;0.003 A, 325 to 1000 nm smoothed</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm pathlength</li><li>Available accessory for test-tubes up to 25mm diameter and 150mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong> USB-A, USB-B</li><li><strong>Description:</strong> GENESYS 30 VIS Spectrophotometer with EU Power Cable</li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> &lt;0.002 A/hr. (At 500 nm after 2 hr. warmup.) Typical &lt;0.004 A/hr. after 1 hr. warmup</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/250 V, 50/60 Hz</li><li><strong>Keypad:</strong> Tactile rubber 23 keys with numeric keypad</li><li><strong>Lamp Life:</strong> 1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500nm. 60 consecutive measurements)<ul><li>≤0.001A at 0A</li><li>≤0.001A at 1A</li><li>≤0.002A at 2A</li></ul></li><li><strong>Optical Design:</strong> Single Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002 A (0 to 0.3 A), 0.5% of ABS reading (0.301 A to 2.5 A)</li><li><strong>Photometric Display:</strong> -3A to +3A, 0 to 200 000 %T, 0 to 9 999 999 C</li><li><strong>Photometric Range:</strong> -3A to +3A</li><li><strong>Photometric Repeatability:</strong> ± 0.002 A (Measured at 1.0 A at 546 nm)</li><li><strong>Printer Included:</strong> Thermal printer accessory with 80 mm width paper</li><li><strong>Scan Speed:</strong> Automatic – up to 1200 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.1%T at 340 nm and 400 nm</li><li>Measured using glass cutoff filters or NaNO₂ at 340 nm</li><li><strong>Warranty:</strong> 2 years standard with 1 year extension upon registration of the instrument with Thermo Fisher Scientific within 6 months of purchase</li><li><strong>Wavelength Accuracy:</strong> ±2 nm</li><li><strong>Wavelength Data Interval:</strong> 1 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±1 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Silicon Photodiode</li><li><strong>Display:</strong> 5 in. 32-bit Color Display, 800 x 480 pixels</li><li><strong>Languages:</strong> English, French, German, Spanish, Italian, Portuguese, Russian, Chinese, Japanese, Korean, Thai, Arabic</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 40 Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 40 Vis Spectrophotometer</strong></h3><p><strong>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.</strong></p><h5><strong>Advanced usability, modern functionality</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg" alt="Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg 234w," sizes="100vw" width="1536" height="1024"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 40 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.005 A</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm cells</li><li>Available accessory for test tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> GENESYS 40 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt;1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002 A at 0.5 A, ±0.004 A at 1.0 A, ±0.008 A at 2.0 A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –3A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001A at 1A measured at 1.0A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Automatic – up to 1800 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.05%T at 340 nm and 400 nm</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li><strong>Light Source:</strong> Tungsten Halogen</li><li><strong>Spectral Bandwidth:</strong> 5 nm</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 140 Vis Spectrophotometer

Perform quantitative measurements in routine industrial QA/QC or university chemistry labs with the automated and network-capable Thermo Scientific™ GENESYS 140 Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 140 Vis Spectrophotometer</strong></h3><p><strong>For advanced teaching labs, R&amp;D, research and life science applications requiring higher throughput or temperature control.</strong></p><h5><strong>Elite performance, enhanced capabilities</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg 225w," sizes="100vw" width="516" height="358"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific: Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 140 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.005 A</li><li><strong>Compartments:</strong> Accommodates (optional):<ul><li>8-position cell changer</li><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> Genesys 140 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt;1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002A at 0.5A, ±0.004A at 1.0 A±0.008 A at 2.0A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –3A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Automatic – up to 1800 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.05%T at 340 nm and 400 nm</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li><strong>Light Source:</strong> Tungsten Halogen</li><li><strong>Spectral Bandwidth:</strong> 5 nm</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 50 UV-Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 50 UV-Vis Spectrophotometer</strong></h3><p><strong>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.</strong></p><h5><strong>Advanced usability, modern functionality</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg" alt="Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg 188w," sizes="100vw" width="534" height="443"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 50 UV-Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.002 A</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100 mm cells</li><li>Available accessory for test-tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> GENESYS 50 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt; 5 years typical, 3 years guaranteed</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –2A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Slow, medium and fast – up to 1600 nm/min.</li><li><strong>Stray Light:</strong> &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO<sub>2</sub>)</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li><strong>Spectral Bandwidth:</strong> 2 nm</li><li><strong>Wavelength Range:</strong> 190 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific Orbitrap Exploris MX Mass Detector

Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information. 

<p><strong>Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information.&nbsp;</strong></p><p>The <strong>Thermo Scientific Orbitrap Exploris MX mass detector</strong> is the perfect fit for deploying routine mass monitoring.</p><p>In addition to high-resolution mass detection provided by Thermo Scientific Orbitrap mass analyzers, this fit for purpose system is simple to operate and compliance ready with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/chromatography/chromatography-learning-center/chromeleon-cds-information/cds-mass-spectrometry-software.html">Thermo Scientific Chromeleon chromatography data system (CDS) software</a>. Consistent performance between the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Thermo Scientific Orbitrap Exploris 240 mass spectrometer</a> and Orbitrap Exploris MX detector ensures seamless method transfer from development to QC.</p><h4><strong>High-resolution accurate-mass (HRAM) data provide utmost confidence for:</strong></h4><ul><li>Sequence confirmation</li><li>Peptide monitoring</li><li>Intact protein analysis (from small to large molecules, reduced mAbs, and intact mAbs under native and denaturing conditions)</li><li>Glycan profiling</li><li>Oligonucleotide and oligonucleotide impurity mass confirmation</li></ul><h3><strong>Highlights of Orbitrap Exploris MX mass detector&nbsp;</strong></h3><div colctrl-id="5-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Simplicity for scientists and technicians</strong></h4><p>Simplify tasks and reduce errors for more ‘right first time’ analyses with Chromeleon CDS, with built in methods, control, data processing, and management. The suite of smart tools makes your work faster and easier, while ensuring reproducible, high-quality mass monitoring.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Accelerate productivity</strong></h4><p>High uptime boosts productivity. Rapid “set and forget” calibration procedures provide consistent mass stability for at least four weeks at prescribed conditions. Instrument status monitoring and optimized pressure control alert users when maintenance is needed, avoiding unnecessary downtime and repeat analyses.</p></div></div></div></div><div colctrl-id="6-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Compliance for competitive advantage</strong></h4><p>Designed for data integrity, data security, and compliance, fully scalable Chromeleon CDS provides comprehensive technical controls to give you a competitive edge in meeting global regulatory requirements.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Consistent results deliver confidence</strong></h4><p>Robust and reliable performance from system to system and site to site ensure confidence in results used to make critical decisions. Industry-proven Orbitrap technology, now purposely designed for mass monitoring in future-proof lab operations.</p></div></div></div></div><div colctrl-id="7-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Seamless method transfer to MAM</strong></h4><p>Easy transfer of sophisticated Multi-Attribute Method (MAM) assays from development to manufacturing bridges the gap between development and QC.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Dedicated, global support team</strong></h4><p>The dedicated, global Thermo Scientific MAM team maximizes your productivity and confidence with installation, training, and fast service and support. Standardized system performance evaluation test (SET) and installation qualification/operational qualification (IQ/OA) thoroughly assess system performance with comprehensive installation and post-maintenance acceptance criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg" alt="Orbitrap Exploris MX mass stability: mass deviation relative to first observed mass in parts-per-million for 9 BSA tryptic peptides monitored over a period of 6 weeks using the new internal recalibration option. Each data point is an average of 19 individual injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg 184w," sizes="100vw" width="1240" height="1052"></p><h3><strong>Accelerate MAM deployment&nbsp;</strong></h3><h4><strong>Seamless knowledge and method transfer</strong></h4><p>Benefit from the end-to-end Thermo Scientific MAM 2.0 workflow approach to comprehensive characterization and monitoring of biotherapeutics from development to commercialization. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Orbitrap Exploris 240</a> mass spectrometer methods, established during development, seamlessly transfer to the Orbitrap Exploris MX detector in manufacturing or by contract partners. In addition to full integration into the high-resolution MAM 2.0 workflow, the usability and low cost of the Orbitrap Exploris MX detector make it the perfect fit for high-throughput QC and contract labs.</p><p>Users can identify and evaluate relevant product quality attributes (PQAs) via high-throughput analytics provided by Thermo Scientific BioPharma Finder software. Connected labs can access data from development through QC using MAM 2.0 powered by Thermo Scientific Ardia Platform. And, PQAs can be monitored throughout the entire drug development process using the MAM target peptide workbook built from the list of identified peptides upon incorporation into a Chromeleon processing method. The processing method along with the instrument method, injection sequence, view setting and report template can be stored into a MAM eWorkflow for easy and direct transfer to QC via Chromeleon CDS Enterprise software.</p><div><h5><strong>MAM 2.0 offers:</strong></h5><ul><li>Fast decision making based on high-confidence PQAs obtained using industry-leading HRAM Orbitrap technology</li><li>Seamless knowledge and method transfer across instruments, departments, and sites using compliance-ready, Chromeleon CDS enterprise software, and Ardia Platform</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg 245w," sizes="100vw" width="1170" height="504"></p><blockquote><p><strong>Thermo Scientific MAM 2.0</strong></p><p>The end-to-end MAM solution for a fully connected, compliance-ready platform. MAM 2.0 includes everything you need to bring your next biotherapeutic candidate straight through to breakthrough.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/pharma-biopharma/biopharmaceutical-analytical-testing/multi-attribute-method.html"><strong>Learn more</strong></a></li></ul></blockquote></div></div></div></div></div>

Thermo Scientific Orbitrap Exploris Mass Spectrometers

Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening, identification, confirmation and quantitation.

<p><strong>Benefit from record-setting performance. With built-in intelligence, sensitivity and selectivity; the Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) for analysis depth to the lowest levels with high quantitative accuracy and precision to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before.</strong></p><h3><strong>Why choose an Orbitrap Exploris mass spectrometer for your laboratory?</strong></h3><p>Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1490"><strong>Orbitrap Exploris MX mass detector</strong></a></h4><p>Accelerate biopharmaceutical commercialization, manufacturing and quality control by easily and reliably obtaining compliance-ready, high-quality mass information with the Thermo Scientific Orbitrap Exploris MX Mass Detector.</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization and QC, intact protein analysis, Multi-Attribute Method (MAM), oligonucleotides, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Absolute and Relative Quantitation (MS)</p><p><strong>Resolution powers: </strong>180,000</p><p><strong>Mass range: </strong>m/z 40–3,000 up to m/z 8,000 with BioPharma option</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg 169w," sizes="100vw" width="1696" height="1575"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/403"><strong>Orbitrap Exploris 120 Mass Spectrometer&nbsp;</strong></a></h4><p>Benefit from best in class high-resolution accurate mass for confident testing, screening and quantification of small molecules with the Thermo Scientific Orbitrap Exploris 120 Mass Spectrometer.&nbsp;&nbsp;</p><p><strong>Typical applications: </strong>Food and environmental testing, metabolomics, forensic toxicology, clinical research, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>120,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 – 3,000</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg" alt="Thermo Orbitrap Exploris 120 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/thumbnail_Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg 171w," sizes="100vw" width="938" height="856"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402"><strong>Orbitrap Exploris 240 Mass Spectrometer&nbsp;</strong></a></h4><p>Get unmatched confidence for discovery and identification, with application versatility and operational simplicity with the Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer.&nbsp;</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization, proteomics, metabolomics, lipidomics, food and environmental research, clinical research</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>240,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>30Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 240 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/thumbnail_Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg 175w," sizes="100vw" width="945" height="841"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/401"><strong>Orbitrap Exploris 480 Mass Spectrometer&nbsp;</strong></a></h4><p>Achieve next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use with the Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer.</p><p><strong>Typical applications: </strong>Shotgun proteomics (LFQ DIA, DDA), single cell proteomics, TMT multiplexing, targeted peptide quantification, metabolomics, biopharmaceutical applications (including HCP and protein characterization).</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>480,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>40Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 480 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/thumbnail_Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg 235w," sizes="100vw" width="880" height="584"></p>

Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system

The Inuvion Core system gives you all the benefits of chemical suppression and higher-pressure operation, with manual eluent preparation.

<h3><strong>Welcome to the evolution of everyday IC reliability</strong></h3><p>The new<strong> Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system</strong> makes ion analysis simpler and more intuitive than ever before. And it delivers exactly what a busy analytical testing lab is looking for: <strong>consistently excellent results-every shift, every day, for every operator.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg 245w," sizes="100vw" width="825" height="498"></p><h3><strong>Start with Inuvion Core</strong></h3><p>The <strong>Inuvion Core system</strong> gives you<strong> </strong>all the benefits of chemical suppression and higher-pressure operation, with manual eluent preparation. You still enjoy the<strong> same robust Dionex Inuvion platform</strong>-easily configurable and economically upgradeable to full-featured capabilities as budget allows.</p><figure class="image"><img style="aspect-ratio:650/650;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_Core_ion_chromatography_system_6c8824cb1d.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_Core_ion_chromatography_system_6c8824cb1d.jpg 156w," sizes="100vw" width="650" height="650"></figure><h3><strong>SIMPLE, INTUITIVE USER EXPERIENCE&nbsp;</strong></h3><ul><li><strong>Smart, function-driven design</strong> lets you quickly and safely access everything on the instrument—no hard-to-reach compartments, and no step stools or extra tools needed!</li><li><strong>Automated startup and shutdown routines</strong> ensure the system is quickly ready for the day’s work without user intervention.&nbsp;</li><li><strong>Built-in how-to videos</strong> reduce training time and simplify setup and operation.</li></ul><h3><strong>ULTRA-RELIABLE PERFORMANCE&nbsp;</strong></h3><ul><li><strong>Advanced high-performance pump technology and electronics</strong> let you take advantage of a wider range of columns and chemistries to improve the speed and quality of your results.</li><li><strong>Fast, flexible, convenient electrolytic suppression: </strong>Just connect the suppressor; no external pump or offline conditioning needed for regeneration.</li><li><strong>Reagent-free IC (RFIC™) capability</strong> makes eluent preparation as simple as “just add water,” while ensuring more consistent results and enabling optimized gradient separations.</li></ul><h3><strong>EASILY CONFIGURABLE AND UPGRADEABLE</strong></h3><ul><li><strong>Versatile, adaptable platform</strong> lets you configure the exact Dionex Inuvion IC system for today’s needs and budget, with confidence that your system will also meet your future needs.</li><li><strong>Multiple options and peripherals </strong>extend IC capabilities, letting you easily—and cost-effectively—adapt to changing sample types and workflow requirements.</li></ul><h3><strong>Simply the best system for everyday ion analysis</strong></h3><figure class="image"><img style="aspect-ratio:1107/755;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg 229w," sizes="100vw" width="1107" height="755"></figure><p><strong>1. Operator-friendly engineering </strong>saves time and reduces errors by allowing easy, unobstructed access to all components, connections and consumables</p><p><strong>2. Compact design </strong>helps you make the most of valuable bench space</p><p><strong>3. Digital gas pressure regulator </strong>increases precision of gas delivery for maximum day-to-day consistency (optional)</p><p><strong>4. Temperature-controlled conductivity cell </strong>improves reproducibility and ensures baseline stability</p><p><strong>5. Vacuum degassing capability</strong> enables more consistent peak identification from smoother baselines</p><p><strong>6. Seal wash pump</strong> reduces maintenance costs when using manually prepared eluents (optional)&nbsp;</p><p><strong>7. Reagent-free IC (RFIC)</strong> saves time and ensures greater day-to-day and operator-to-operator consistency, while also allowing the use of gradient separations</p><p><strong>8. Electrolytic suppression</strong> entirely eliminates the need for manual regenerant preparation (or, opt for a continuously regenerated, always- ready-to-go chemical suppressor)</p><p><strong>9. Thermo Scientific™ Dionex™ IC PEEK Viper™ fittings</strong> ensure confident, low dead volume connections every time—no tools required! (optional)</p><p><strong>10. Integrated column heater</strong> minimizes the impact of fluctuations in lab temperature that can adversely affect results (optional)</p><p><strong>11. 6- or 10-port auxiliary valve</strong> lets you automate in-line sample preparation</p><p><strong>12. State-of-the-art high-pressure pump (up to 5000 psi)</strong> lets you increase flow rates with smaller particle columns to reduce run times or add additional separation capacity with longer column length</p><p><strong>13. System self-diagnostics </strong>automatically detect any issues with hardware and consumables</p><p><strong>14. Self-service capabilities and user-swappable parts</strong> help you get back up and running in a hurry Automatic eluent monitor alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)</p><p><strong>15. Automatic eluent monitor </strong>alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)</p><h3><strong>Flexible, adaptable Dionex Inuvion platform</strong></h3><p>You can <strong>configure your Dionex Inuvion system</strong> to meet specific analytical and budget requirements, and easily reconfigure as needed. Multiple configurations and peripherals extend IC capabilities, letting you easily-and cost-effectively-adapt to changing sample types and workflow requirements.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg 245w," sizes="100vw" width="1246" height="572"></p><h3><strong>Consistently excellent results every day</strong></h3><p>The <strong>Dionex Inuvion IC system supports a full range of columns </strong>to improve the speed and quality of results, for both<strong> anions and cations.</strong> Expanded capabilities, such as Reagent-Free IC (RFIC), enhance operator-to-operator consistency, while enabling the use of eluent gradients to optimize separations.</p><h4><strong>Rapid results</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 1. Elution of seven common inorganic anions using carbonate eluent. Short columns and fast flow rates let you reduce run times." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg 245w," sizes="100vw" width="1622" height="739"></p><h4><strong>Exceptional reproducibility</strong></h4><figure class="image"><img style="aspect-ratio:1693/754;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_3_Ukazka_reprodukovatelnosti_analyzy_zakladnich_aniontu_40_nastriku_Spickova_technologie_cerpadla_a_elektronika_pristroje_poskytuji_konzistentni_a_reprodukovatelne_vysledky_f66e7430cf.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 2. Overlay of 40 replicate injections. State-of-the-art pump technology and Electronics deliver consistent, reproducible results." width="1693" height="754"></figure><h4><strong>Increased sensitivity and consistency</strong></h4><figure class="image"><img style="aspect-ratio:1860/836;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_4_Ukazka_vysoce_citlive_analyzy_aniontu_ve_vodach_Eluent_generator_KOH_a_elektrolyticka_suprese_snizuji_pozadi_cimz_dochazi_ke_zvyseni_citlivosti_da55facfd0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 3. Determination of inorganic anions in drinking and surface waters. KOH eluent generation and electrolytic suppression yield lower backgrounds for greater sensitivity and increased day-to-day consistency." width="1860" height="836"></figure><h4><strong>Method optimization</strong></h4><figure class="image"><img style="aspect-ratio:1799/889;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_5_Ukazka_optimalizace_metody_zkraceni_doby_analyzy_pouzitim_gradientu_mobilni_faze_fc1fbe9cb0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 4. Comparison of isocratic and gradient runs using eluent generation. Reduce run length and sharpen later-eluting peaks with the same ease as an isocratic run with no need for two separate eluents or a proportioning pump." width="1799" height="889"></figure><h3><strong>Compliance-ready Chromeleon CDS</strong></h3><p>A trusted industry standard all over the world, ”Simply Intelligent”<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software </strong></a>brings out the full power of the Dionex Inuvion IC system. Easy to learn and easy to use, it automates and optimizes daily tasks and helps even inexperienced users achieve consistent, high quality results.</p><p><img style="aspect-ratio:3700/1660;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Thermo_Scientific_Dionex_Inuvion_2_303a6eb73b.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system." width="3700" height="1660"></p>

Thermo Scientific™ LipidSearch™ Software

Thermo Scientific™ LipidSearch™ software provides accurate identification of lipids and automatically integrates complex data into a concise report.

<h3><strong>The promise of lipidomics</strong></h3><p><strong>Lipidomics is a new field of study crucial for understanding cellular physiology and pathology. The application of lipidomic profiling to disease phenotype analysis is a rapidly growing aspect of translational medical research. Identification of unique lipid biomarkers has the potential to distinguish healthy individuals from individuals at risk for disease, detect diseases earlier, and facilitate development of personalized treatments.</strong></p><p>Liquid chromatography combined with mass spectrometry (LC/MS) is a widely adopted technique for lipidomics analyses. Relative and absolute quantitation, and identification, of lipids from biological samples requires sophisticated software with an extensive, comprehensive database.<strong> Thermo Scientific™ LipidSearch™ software provides accurate identification of lipids and automatically integrates complex data into a concise report. With its easy-to-use web-based interface, it dramatically reduces data analysis time.</strong></p><figure class="image"><img style="aspect-ratio:915/494;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_2_73b7d0a9e2.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Easy-to-use web browser interface for automated and reliable identification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_2_73b7d0a9e2.jpg 245w," sizes="100vw" width="915" height="494"></figure><h3><strong>Automated identification and relative quantitation with LipidSearch software</strong></h3><p>LipidSearch software, developed jointly by Professor Ryo Taguchi and MKI, (Tokyo, Japan), is a powerful new tool for<strong> automatic identification and relative quantification of cellular lipid molecular species from large amounts of mass spectrometric data obtained in nano-infusion or LC-MS experiments. </strong>Using the industry-leading, high-resolution, accurate-mass <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=orbitrap"><strong>Thermo Scientific Orbitrap™ technology</strong></a> with exclusive<strong> LipidSearch software</strong>, the most accurate and confident lipid profiles and identifications can be achieved more quickly than ever before.</p><ul><li><strong>Compatible with</strong> data acquired from Thermo Scientific™ triple quadrupole, ion trap, and Orbitrap mass spectrometers</li><li><strong>Largest lipid database</strong> containing <strong>&gt;1.5 million</strong> lipid ions and their predicted fragment ions</li><li><strong>Identification algorithms</strong> for product ion, precursor ion and neutral loss scans</li><li><strong>Alignment of lipid data</strong> obtained from multiple<strong> LC-MS and MS<sup>n</sup> experiments</strong></li><li><strong>Relative quantitation </strong>of identified lipid precursors in either LC-MS or infusion experiments</li></ul><p>The database contains <strong>defined structures and includes more than 1.5 million lipid ions and their predicted fragment ions. </strong>Fragmentation patterns are calculated and improved by using expert knowledge based on experimental results. Lipid adduct ions and MS<sup>n</sup> fingerprints are also included. Data are stored in XML files and are easily customized.</p><figure class="image"><img style="aspect-ratio:990/497;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_3_91f1458663.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Lipid fragment ions related to lipid headgroup, fatty acids, and backbone." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_3_91f1458663.jpg 245w," sizes="100vw" width="990" height="497"></figure><h3><strong>LipidSearch software provides an easy-to-use, automated workflow</strong></h3><p><strong>From peak detection to relative quantitaiton and identification, LipidSearch software provides an easy-to-use, automated workflow.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_4_2043f3d667.jpg" alt="Thermo Scientific™ LipidSearch™ Software: LipidSearch software provides an easy-to-use, automated workflow." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_4_2043f3d667.jpg 245w," sizes="100vw" width="1122" height="530"></p><h4><strong>Step 1 Data Analysis Module — Peak Detection</strong></h4><ul><li><strong>Raw data file reading</strong></li><li><strong>Smart peak detection</strong></li></ul><p>The peak detection engine implemented in<strong> LipidSearch software </strong>can handle<strong> different MS experiments and platforms. </strong>The combination of unique peak detection algorithms, tailored for each experiment and instrument type, and mass spectral processing functions enables accurate peak detection.</p><p><strong>LipidSearch software provides two different identification algorithms as well as scoring algorithms:</strong></p><ul><li><strong>A group-specific algorithm</strong> identifies lipids based on the polar head groups or fatty acids using a combination of precursor ion scanning and neutral loss scans from lipids mixtures.</li><li><strong>The comprehensive ID algorithm</strong> is used for product ion scans and can discriminate each lipid by matching the predicted fragmentation pattern stored in the database.</li><li>LipidSearch software also provides a <strong>set of scoring algorithms</strong> to filter out lower probability results</li></ul><h4><strong>Step 2 Identification Module — Lipid Identification</strong></h4><ul><li><strong>Group-specific ID (targeted)</strong></li><li><strong>Comprehensive ID (untargeted)</strong></li><li><strong>Scoring algorithms</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_5_0abd534dd5.jpg" alt="Thermo Scientific™ LipidSearch™ Software: PG 17:0/17:0 Search Results." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_5_0abd534dd5.jpg 245w," sizes="100vw" width="1003" height="456"></p><h4><strong>Step 3 Quantitation Module — Alignment and Quantitation</strong></h4><ul><li><strong>XIC peak integration</strong></li><li><strong>Retention time alignment</strong></li><li><strong>Relative quantitation</strong></li><li><strong>Statistical analysis</strong></li></ul><p>Prior to quantitation, lipid results from each sample are aligned within a retention time window. Identified lipids are quantified by detecting their precursor ions from full-scan MS and integrating extracted ion chromatograms.</p><p>Accurate peak areas are computed by denoising and smoothing the peak profiles prior to separating any partially overlapped peaks. Comparative analysis is then carried out between the multiple sample and control groups using <strong>t-test statistics.</strong> The mean peak area result for each group is displayed in a “box and whisker” plot.</p><figure class="image"><img style="aspect-ratio:1129/909;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_6_196073034c.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Alignment results for CoQ7, [M+NH4]+." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_6_196073034c.jpg 194w," sizes="100vw" width="1129" height="909"></figure><p>Using the industry-leading, high-resolution, accurate-mass <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=orbitrap"><strong>Thermo Scientific™ Orbitrap™ technology</strong></a><strong> </strong>with exclusive<strong> LipidSearch software</strong>, achieve the most accurate and confident lipid profiles and identifications more quickly than ever before. For targeted quantitation, LipidSearch software also supports the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=tsq"><strong>Thermo Scientific TSQ triple quadrupole MS systems</strong></a><strong>. </strong>For both lipidomics workflows, LipidSearch software <strong>automatically integrates complex data into a combined report to dramatically reduce data analysis.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_7_f6a5102f0a.jpg" alt="Thermo Scientific™ LipidSearch™ Software." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_7_f6a5102f0a.jpg 245w," sizes="100vw" width="924" height="570"></p><h5><strong>Minimum/Recommended PC Requirements</strong></h5><ul><li><strong>OS: </strong>Microsoft® Windows® 7 Professional (x64) or Windows 8 (x64); English language<br><strong>CPU: </strong>Quad - or multi-core CPU, 2 GHz or higher<br><strong>Memory:</strong> 8 GB RAM or higher<br><strong>HDD: </strong>50 GB free space for LipidSearch software; 500 GB hard drive<br>SSD (optional): 50 GB free space for LipidSearch software; 256 GB solid-state drive</li></ul>

Thermo Scientific™ ProSightPD Software

Perform comprehensive analysis of top-down proteomics data with Thermo Scientific™ ProSightPD™ software.

<div container-id="cq-colctrl-lt8" comp-id="B1"><div><h3><strong>Confident proteoform identification and characterization</strong></h3></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div><p><strong>Uncover and confidently characterize proteoforms within complex matrices, including proteoforms containing truncations, alternative splices, and post-translationally modified proteins.</strong>&nbsp;</p><p>Extensive searches make use of <strong>various fragmentation methodologies (e.g., ETD, HCD, UVPD)</strong>, allowing you to make full use of the high-quality <strong>MS/MS data</strong> obtained when searching against the<strong> full biological complexity present in UniProt. </strong>With<strong> Thermo Scientific ProSightPD software, </strong>see what is really there and make the most of your data.</p><div><h3><strong>Key benefits</strong></h3><h5><strong>Sophisticated tools at your fingertips</strong></h5><p><strong>Utilize several search modes</strong> for identifying intact and truncated proteins. ProSightPD software offers the ability to search entire raw files or quickly select specific MS<sup>2</sup> scans for further searching. Information in UniProt enables the identification of modified or processed proteins.</p><h5><strong>Remove the guesswork from database management</strong></h5><p><strong>Import result files </strong>from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong> Proteome Discoverer</strong></a><strong> software</strong> or entire proteomes via UniProt formatted XML and FASTA files with Database Manager. Fully customize imported databases using thr<strong> ProSight Annotator GUI </strong>to characterize PTMs, disulfide bonds, mutations and truncations.</p><h5><strong>Intuitive user interface</strong></h5><p><strong>Analyze large datasets</strong> with many data files using <strong>ProSightPD nodes for </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Proteome Discoverer</strong></a><strong> software. </strong>Create multi-step searches and utilize built in results interpretation tools.</p><h3><strong>Search Types</strong></h3><div><p><strong>Different database search modes</strong> allow you to optimize data interrogation based upon your experimental needs. Searches are driven through the use of a node-based system allowing flexibility of experimental design.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_8a96430534.jpeg" alt="Thermo Scientific™ ProSightPD Software: ProSightPD Database Searching." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_8a96430534.jpeg 245w," sizes="100vw" width="960" height="540"></p><div><p>Further information relating to the two search modes, Absolute Mass Search and Biomarker Search.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_2_01f6c31076.jpeg" alt="Thermo Scientific™ ProSightPD Software: Complementary Search Types: Digging Deeper in Complex Samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_2_01f6c31076.jpeg 245w," sizes="100vw" width="960" height="540"></p><h3><strong>Database Management</strong></h3><div><p><strong>Confident proteoform identification </strong>is realized through the use of XML-formatted databases, ensuring that nothing is missed.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_3_db19f0cee9.jpeg" alt="Thermo Scientific™ ProSightPD Software: Database Manager: Add remove isoforms and PTMs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_3_db19f0cee9.jpeg 245w," sizes="100vw" width="960" height="540"></p><div><p><strong>Improvements to data visualization</strong> ensure that you can better interact with and interpret your data, minimizing the time taken to deliver actionable results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_4_575aa9dff5.jpeg" alt="Thermo Scientific™ ProSightPD Software: LFQ Visualizations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_4_575aa9dff5.jpeg 245w," sizes="100vw" width="960" height="540"></p><h3><strong>ProSightPD nodes</strong></h3><p><strong>Top-Down Workflows:</strong> ProSightPD&nbsp;includes templates to easily build the most commonly used-top down data analysis workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_5_4f28a7434d.jpg" alt="Thermo Scientific™ ProSightPD Software: ProSightPD 2.0: Tailored Workflows for All Types of Data." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_5_4f28a7434d.jpg 245w," sizes="100vw" width="740" height="400"></p></div></div></div></div></div></div></div>

Thermo Scientific™ Mass Frontier™ Spectral Interpretation Software

Transform your mass spectral data into knowledge quickly and easily using Thermo Scientific™ Mass Frontier™ spectral interpretation software. 

<p><strong>Transform your mass spectral data into knowledge quickly and easily using Thermo Scientific™ Mass Frontier™ spectral interpretation software. Take the challenge out of small molecule structural elucidation for metabolism, metabolomics, forensics, natural products, impurities, and degradants studies.</strong></p><p><strong>High-quality, curated experimental spectral and fragmentation libraries</strong> simplify the deconvolution, evaluation, interpretation and management of infusion, and LC, nominal mass, or high resolution mass spectral data. Streamline building and curating your library and sharing it with your colleagues and collaborators. <strong>Mass Frontier software complements Thermo Scientific™ </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759"><strong>Compound Discoverer™</strong></a><strong> software</strong> for routine-based small molecule discovery and identification.</p><h3><strong>See what's really there</strong></h3><ul><li>Deconvolute your nominal mass or high resolution LC-MS<sup>n</sup> or infusion data.</li><li>Capture all of your data, no matter how complex, with simple interrogation of all components.</li></ul><h3><strong>Know more unknowns</strong></h3><ul><li><strong>Transform </strong>your small molecule unknown data into actionable knowledge.</li><li><strong>Search </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764"><strong>mzCloud™</strong></a><strong> mass spectral library</strong>, the world's most comprehensive and structurally diverse fully curated mass spectral database.</li><li><strong>Maximize the utility of your MS<sup>n</sup> data</strong> for the confident identification of unknown with spectral tree searching.</li><li><strong>Enhance your confidence</strong> in proposed unknown candidate structures by using <strong>Thermo Scientific™ </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1762"><strong>mzLogic™</strong></a><strong> algorithm</strong>, based on similarity searching to millions of fragment annotated spectra.</li></ul><figure class="image"><img style="aspect-ratio:740/405;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_3_167df5dc63.jpg" alt="Thermo Scientific™ Mass Frontier™ Spectral Interpretation Software: mzCloud mass spectral library." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_3_167df5dc63.jpg 245w," sizes="100vw" width="740" height="405"></figure><h3><strong>Go from spectra to structure</strong></h3><ul><li>The HighChem Fragmentation Library™ includes <strong>&gt;54,000 fragmentation schemes, ∼ 220,000 individual reactions, and 256,000 chemical structures</strong>, all from peer-reviewed literature.</li><li>Spectral trees are fully annotated with fragmentation structures.</li><li><strong>FISh (fragment ion search) detection</strong> enables detection of structurally related compounds, indicating the site of transformations for drug metabolites.</li></ul><figure class="image"><img style="aspect-ratio:740/350;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_2_37f024ef32.jpg" alt="Thermo Scientific™ Mass Frontier™ Spectral Interpretation Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_2_37f024ef32.jpg 245w," sizes="100vw" width="740" height="350"></figure><h3><strong>Build your pathways</strong></h3><ul><li>Interactively search more than <strong>370 curated</strong> <strong>biochemical pathways</strong> with<strong> Thermo Scientific™ Metabolika™</strong> pathway mapping.</li><li>Create and understand fragmentation pathways and mechanisms using advanced fragmentation scheme knowledge base drawn from more than 99% of published fragmentation pathways.</li></ul><figure class="image"><img style="aspect-ratio:740/410;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_4_7d1b4f8671.jpg" alt="Thermo Scientific™ Mass Frontier™ Spectral Interpretation Software: An example of an included Metabolika biological pathway, which can be fully edited and added to." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_4_7d1b4f8671.jpg 245w," sizes="100vw" width="740" height="410"></figure><h3><strong>Store, share and utilize your knowledge</strong></h3><ul><li><strong>Translate your data into actionable knowledge:</strong> create, curate and store your own spectral libraries and pathways.</li><li><strong>Ensure the highest quality proprietary libraries</strong> with full curation and mass recalibration tools.</li></ul><figure class="image"><img style="aspect-ratio:740/345;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_5_e89924a0b7.jpg" alt="Thermo Scientific™ Mass Frontier™ Spectral Interpretation Software: The new curator module automates spectral tree curation, fragment annotation and mass recalibration for your proprietary compounds to build a high quality curated local MSn library." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Mass_Frontier_Spectral_Interpretation_Software_5_e89924a0b7.jpg 245w," sizes="100vw" width="740" height="345"></figure>

Products from Thermo Fisher Scientific - page 13

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