Size exclusion chromatography (SEC) columns are highly sensitive to operating conditions, including the composition of the mobile phase and the nature of analyzed samples. Improper handling and unsuitable method conditions can significantly reduce column lifetime. This guide summarizes common causes of column degradation and provides practical recommendations for extending column lifespan.<h3>Common Causes of Reduced Column Lifetime</h3>Several factors can negatively affect the performance and durability of SEC columns:<h4>1. Contamination of the Stationary Phase</h4><ul><li>Accumulation of impurities from samples or mobile phases</li><li>Microbial growth due to infrequent replacement of mobile phase</li></ul><h4>2. Stationary Phase Degradation</h4><ul><li>Caused by unsuitable pH conditions</li><li>Exposure to inappropriate temperatures</li><li>Use of harsh chemical agents</li></ul><h4>3. Improper Column Storage</h4><ul><li>Incorrect storage conditions may reduce column performance over time</li></ul><h4>4. Column Inlet Blockage</h4><ul><li>Particulates from insufficiently prepared samples can clog the column inlet</li></ul><h3>Preventive Measures and Best Practices</h3><h4>1. Preventing Contamination</h4><ul><li>Use high-quality solvents</li><li>Prepare buffer solutions daily</li><li>Filter both mobile phases and samples before use</li><li>Perform regular column cleaning</li></ul>Important note: Not all SEC columns can be reverse flushed. For example:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and Yarra SEC-X7 columns must not be backflushed</li></ul><h4>2. Protecting the Stationary Phase</h4><ul><li>Operate within recommended pH and temperature limits</li><li>When using strong denaturants (e.g., urea or SDS):<ul><li>Perform immediate and thorough water flushing after use</li></ul></li></ul><h4>3. Proper Column Storage</h4>Before storage always clean the column thoroughly<ul><li>Two storage options:<ul><li>Buffer with bacteriostatic agent (e.g., sodium azide)</li><li>No buffer: water with 20% methanol</li></ul></li></ul>When introducing methanol:<ul><li>Use low flow rates</li><li>Prevent pressure spikes that could damage the column</li></ul>When restarting the column:<ul><li>Consider that the column contains 20% methanol</li><li>Adjust flow conditions accordingly</li></ul><h4>4. Preventing Column Inlet Clogging</h4>Use protective components:<ul><li>Guard column</li><li>Precolumn</li></ul>These act as barriers to prevent contamination of the analytical column.<h5>Types of Protection:</h5><ul><li>Security guard cartridges<ul><li>Minimal added length</li></ul></li><li>Short precolumns<ul><li>Same stationary phase as analytical column</li></ul></li></ul><h5>Key Advantage:</h5><ul><li>Essential for columns that cannot be backflushed</li><li>Protects against:<ul><li>Poorly centrifuged samples</li><li>Particulate contamination</li><li>Dirty sample matrices</li></ul></li></ul><h3>Conclusion</h3>Extending the lifetime of SEC columns requires careful attention to:<ul><li>Sample preparation</li><li>Mobile phase quality</li><li>Operating conditions</li><li>Storage procedures</li><li>Use of protective accessories</li></ul>Implementing these practices helps prevent contamination, degradation, and mechanical damage, ensuring consistent column performance and longer service life.

Extending the Lifetime of Size Exclusion Columns

<h4>Let’s begin with a simple question: what do we actually mean by high throughput?</h4>In the laboratory, high throughput usually refers to the ability to analyze tens, hundreds, or even thousands of samples per day, either across a laboratory workflow or on a single analytical system. Achieving this requires more than just a fast method. It depends on reliable instrumentation, automation, sufficient sample capacity, and smart system design that minimizes idle time between runs.In this article, we look at the key requirements for high-throughput HPLC analysis and how they are addressed in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2371">HTQC system</a>.<h4>Speed</h4>High-throughput HPLC workflows typically rely on short methods. This includes both the chromatographic runtime and the cycle time of the injection module.With the HTQC system, fast gradient applications up to 1200 bar can be implemented using two integrated UHPLC high-pressure gradient pumps.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_2_HPG_pumps_of_HTQC_system_left_P_8_1_L_max_pressure_1240_bar_right_P_6_1_L_max_pressure_1000_bar_4d4873ff2f.jpg" alt="KNAUER: Fig. 1–2 HPG pumps of HTQC system, left: P 8.1L (max. pressure 1240 bar), right: P 6.1L (max. pressure 1000 bar)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_2_HPG_pumps_of_HTQC_system_left_P_8_1_L_max_pressure_1240_bar_right_P_6_1_L_max_pressure_1000_bar_4d4873ff2f.jpg 245w," sizes="100vw" width="1266" height="386">We will not discuss the full details of transferring classical HPLC methods to fast, high-resolution UHPLC methods here, but UHPLC implementation can dramatically reduce total analysis time.Let’s use a hypothetical example. Imagine Giorgia, a randomly chosen name, working in pharmaceutical quality control in 1998. She must have travelled back in time, because she would have been too young to work in a lab then, but the example still works. With a classical HPLC method, each sample takes approximately 75 minutes, including equilibration, analysis, and backflushing of a 250 × 4.6 mm ID column. This type of method has been common in QC laboratories around the world. During an eight-hour working day, Giorgia can analyze around six samples.Now let’s transfer the same method to UHPLC. The improvement is substantial. Analysis time, equilibration time, and backflushing time are all reduced to under six minutes per sample. Giorgia can now process around 90 samples in one working day.You may wonder why the HTQC system requires two UHPLC gradient pumps. We will come back to that shortly.The next way to increase speed is to reduce injector cycle time. Injection procedures and washing steps can sometimes take a significant amount of time. This is where the LH 8.1 liquid handler becomes important.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_3_KNAUER_LH_8_1_Liquid_Handler_77ab506ca6.webp" alt="KNAUER: Fig. 3 KNAUER LH 8.1 Liquid Handler" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_3_KNAUER_LH_8_1_Liquid_Handler_77ab506ca6.webp 129w," sizes="100vw" width="800" height="971">The LH 8.1 supports overlapped injections. This means that the next injection can already be prepared while the current analysis is still running. Necessary cleaning steps of the injection system are still maintained, but idle time is reduced.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Schematic_buildup_of_injections_with_and_without_overlapped_injections_af089ba652.webp" alt="KNAUER: Fig. 4 Schematic buildup of injections with and without overlapped injections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Schematic_buildup_of_injections_with_and_without_overlapped_injections_af089ba652.webp 245w," sizes="100vw" width="1920" height="452">Let’s return to Giorgia. By using overlapped injections in her QC method, she saves about one minute per sample. This increases her capacity to approximately 105 samples per day.<h4>Sample Throughput</h4>A high-throughput system must also be able to handle and store a sufficient number of samples. Here, the LH 8.1 provides another major advantage. In the long-rail version, it can be equipped with up to three robotic coolers.With three coolers, each containing three drawers, the system can hold nine 10 × 13 sample trays. That means a total capacity of 1170 vials. The samples are also temperature controlled.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_5_6_Robotic_Cooler_with_two_10x6_trays_LH_8_1_887_version_with_two_Robotic_Coolers_855b10c2a3.jpg" alt="KNAUER: Fig. 5–6 Robotic Cooler with two 10x6 trays, LH 8.1 (887 version) with two Robotic Coolers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_5_6_Robotic_Cooler_with_two_10x6_trays_LH_8_1_887_version_with_two_Robotic_Coolers_855b10c2a3.jpg 245w," sizes="100vw" width="1262" height="503">Combined with the low eluent consumption typical of UHPLC, this large sample capacity makes it possible to extend operation from an eight-hour workday to 24-hour operation.For Giorgia and her QC lab, this changes everything again. The same UHPLC method, including overlapped injections, can now run continuously around the clock. This allows approximately 320 samples to be processed per day.Is that enough? Maybe not. As the blog title suggests: we just can’t get enough.<h4>Automation</h4>In addition to a standard 6-port, 2-position injection valve, the HTQC system includes an additional valve. This special column-switching and backflushing valve enables two columns to be operated alternately within a single UHPLC system.While one column is used for analysis, the second column can be backflushed and equilibrated in the reverse flow direction at the same time. This is particularly useful for reducing matrix effects, which are often encountered in QC laboratories when complex sample matrices are analyzed. It also helps reduce overall method runtime.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_7_Exemplary_flow_scheme_of_special_column_switching_valve_d837a71117.webp" alt="KNAUER: Fig. 7 Exemplary flow scheme of special column switching valve" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_7_Exemplary_flow_scheme_of_special_column_switching_valve_d837a71117.webp 127w," sizes="100vw" width="735" height="905"><h4>Parallel Processing</h4>Remember the earlier question: why does the system use two UHPLC gradient pumps?The reason is now clear. The second gradient pump is required to backflush one column while the other column is connected to the analytical flow path through the special switching valve.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_8_Schematic_illustration_of_column_cleaning_equilibration_and_analysis_with_special_backflush_valve_eae2e6f705.webp" alt="KNAUER: Fig. 8 Schematic illustration of column cleaning, equilibration and analysis with special backflush valve" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_8_Schematic_illustration_of_column_cleaning_equilibration_and_analysis_with_special_backflush_valve_eae2e6f705.webp 245w," sizes="100vw" width="1920" height="281">Giorgia immediately ordered a second UHPLC pump and the special valve for her QC lab. She now has a complete HTQC system and is very pleased with the result.Why? Let’s calculate it one final time. With all automation features in place, Giorgia can clean one column while the other column analyzes a sample. This enables her to process an impressive 600 samples per day.Compared with the classical HPLC method she used in 1998, the daily sample throughput has increased from six samples to 600 samples. That is a 100-fold increase.<h4>Summary</h4>We started with a question, so let’s end with one too: what steps increased the throughput?First, the method was scaled down from classical HPLC to a fast UHPLC approach. Then, a second pump and a special column-switching and backflushing valve were added to the UHPLC setup. Together with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1454">LH 8.1 liquid handler</a> and its exceptional sample capacity, the HTQC system becomes a flexible and powerful configuration for high-throughput analysis.The system can also be coupled to nearly any detector used in HPLC. Because of its ability to operate at very high speed, it is especially well suited for mass spectrometric methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_9_Complete_flow_scheme_of_HTQC_system_with_highlighted_system_components_edef36cb42.webp" alt="KNAUER: Fig. 9 Complete flow scheme of HTQC system with highlighted system components" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_9_Complete_flow_scheme_of_HTQC_system_with_highlighted_system_components_edef36cb42.webp 161w," sizes="100vw" width="800" height="775"><blockquote>For further information on this topic, please contact our authors: kramer@knauer.net, m.myrach@knauer.net</blockquote>

Introducing Our System Solution for High Throughput Quality Control – HTQC

News from LabRulezLCMS Library - Week 19, 2026

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Thermo Scientific Acclaim 120 C18 Columns

Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns.

Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns. These columns feature an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver symmetrical peak shapes. The unique bonding chemistry yields excellent surface coverage to provide highly predictable separations that are unaffected by secondary interactions. Use these high-efficiency columns for maximum resolution in a wide variety of applications.<h5>High Performance Reversed-Phase Columns for Reproducible Results</h5><ul><li>Very high surface coverage and very low metal content together result in columns with excellent efficiencies</li><li>Reproducible column-to-column performance</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 11600 psi (800 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C18</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon load: 18%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim 300 C18 HPLC Columns

Achieve high-resolution peptide mapping and protein separations with Thermo Scientific™ Acclaim™ 300 C18 LC Columns.

Achieve high-resolution peptide mapping and protein separations with Thermo Scientific™ Acclaim™ 300 C18 LC Columns. The 3μm ultrapure silica particles reduce the limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns for faster separations.<h5>High-Resolution Separation of Proteins and Peptides</h5><ul><li>High-performance C18 bonding chemistry on 300Å pore silica</li><li>Application tested for suitability in peptide mapping</li><li>Reproducible for dependable results</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 .5 and 7.5</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 6000 psi (414 bar)</li><li>Packing Material: Ultrapure Silica</li><li>Stationary Phase: C18</li><li>Surface Area: 100 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon load: 8%</li><li>Phase: Reversed phase</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim 120 C8 HPLC Columns

For reversed-phase separation of analytes with moderate hydrophobicity, use Thermo Scientific™ Acclaim™ 120 C8 LC columns.

For reversed-phase separation of analytes with moderate hydrophobicity, use Thermo Scientific™ Acclaim™ 120 C8 LC columns. These high-performance columns provide selectivity that is similar to Acclaim 120 C18 columns with a predictable elution order but less retention. Compounds elute more quickly, yielding faster separations. The unique chemistry of densely bonded C8 ligands on an ultrapure, spherical, porous silica substrate gives the Acclaim 120 C8 columns excellent efficiencies.<h5>High Performance Reversed-Phase Columns for the Separation of Small Molecules</h5><ul><li>Low silanol activity for excellent peak shapes</li><li>Reproducible column-to-column performance</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Porous, Ultrapure Silica</li><li>Stationary Phase: C8</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L7</li><li>Carbon load: 11%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim C30 HPLC Columns

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. 

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development.<h5>High Shape Selectivity for the Separation of Structurally Related Isomers</h5><ul><li>Unique selectivity complementary to other reversed-phase columns</li><li>High efficiency and rugged packing</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 8000 psi (550 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C30</li><li>Surface Area: 200 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L62</li><li>Carbon load: 13%</li><li>Phase: Reversed phase</li><li>Pore Size: 200 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim HILIC-10 HPLC Columns

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. 

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.<h5>Rugged and Reproducible Separations of Highly Polar Compounds:</h5><ul><li>Unique selectivity, complementary to reversed-phase columns</li><li>Enhanced sensitivity for MS detection</li><li>Hydrolytically stable</li><li>Rugged column packing</li><li>Broad application range, including separation of hydrophilic drugs and drug metabolites</li></ul>Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.<h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Silica</li><li>Surface Area: 300 m²/g</li><li>Temperature: 70°C</li><li>USP Type: N/A</li><li>Carbon load: 8%</li><li>Phase: HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Polar Advantage HPLC Columns

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns.

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes.&nbsp;In addition, the mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negates the problems of dewetting common with conventional C18 reversed-phase columns. Use Acclaim PA columns for efficient separations of nucleic acid and bases or pharmaceutical compounds using highly aqueous mobile phases.<h5>Unique Selectivity for the Separation of Polar and nonpolar Analytes</h5><ul><li>Low silanol activity for excellent peak shape with basic compounds</li><li>High selectivity for hydrophobic aromatic molecules</li><li>Compatible with 100% aqueous mobile phase</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li><li><h4>Specifications</h4></li><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar Embedded</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L60</li><li>Carbon load: 17%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim VANQUISH C18 UHPLC Columns

Achieve even greater power of separation, speed, and throughput using Thermo Scientific Acclaim VANQUISH C18 UHPLC columns.

Achieve even greater power of separation, speed, and throughput using Thermo Scientific™ Acclaim™ VANQUISH™ C18 UHPLC columns. These reversed phase columns were developed in conjunction with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3&amp;search=vanquish">Vanquish UHPLC system</a> to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges in liquid chromatography (LC) and LC-MS analyses.<h5>Delivering Powerful Separations</h5>The expanded range of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver:<ul><li>Better Separations: high efficiency enables separation of very complex mixture</li><li>More Results: High efficiency at high flow rates</li><li>Easier Interactions: Seamless workflow solution, for simple and easy separations</li><li>Wider Capabilities: Wide range of column selectivities and formats</li></ul><h4>Specifications</h4><ul><li>Column type: Reversed Phase</li><li>Endcapped: yes</li><li>Max. Pressure: 21756 psi (1500 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: C18</li><li>USP Type: L1</li><li>Surface Area: 300 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 18%</li><li>Phase: Reversed Phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim VANQUISH Polar Advantage II UHPLC Columns

Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run using Thermo Scientific Acclaim VANQUISH PA2 UHPLC columns.

Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run using Thermo Scientific™ Acclaim™ VANQUISH™ PA2 UHPLC columns. These reversed-phase columns were developed in conjunction with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3&amp;search=vanquish">Vanquish UHPLC system</a> to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges in liquid chromatography (LC) and LC-MS analyses. These Polar Advantage II columns provide selectivity that is complementary to conventional C18 columns.<h5>Delivering Powerful Separations</h5>The expanded range of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver:<ul><li>Better Separations: high efficiency enables separation of very complex mixture</li><li>More Results: High efficiency at high flow rates</li><li>Easier Interactions: Seamless workflow solution, for simple and easy separations</li><li>Wider Capabilities: Wide range of column selectivities and formats</li></ul><h4>Specifications</h4><ul><li>Column type: Reversed Phase</li><li>Endcapped: yes</li><li>For use with: LC/MS and RSLC</li><li>Max. Pressure: 21756 psi (1500 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar</li><li>USP Type: L60</li><li>Surface Area: 300 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 17%</li><li>Phase: Reversed Phase</li><li>Pore Size: 120 Å</li><li>pH: 1.5 to 10</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Syncronis Phenyl  HPLC Columns

Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. 

Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. High surface area silica, dense stationary phase coverage, and double end-capping minimize secondary interactions and deliver reproducible high-resolution separations. The Phenyl-bonded aromatic phase offers alternative selectivity to C18 columns and is particularly useful for optimized retention of aromatic analytes.Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.<h5>Highly Reproducible Separations</h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li><li>Double endcapped for extra surface coverage</li></ul><h5>Exceptional Column-to-Column Consistency</h5><ul><li>Manufactured, packed and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5>Alternative Selectivity</h5><ul><li>Enhanced retention of aromatic compounds</li></ul><h5>Available in Three Particle Sizes</h5><ul><li>1.7μm for rapid ultrahigh-performance LC (UHPLC) separations</li><li>3μm and 5μm for more traditional HPLC analysis</li></ul><h4>Specifications</h4><ul><li>Column type: Reversed Phase</li><li>Endcapped: Yes</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Phenyl</li><li>USP Type: L11</li><li>Surface Area: 320 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 11%</li><li>Phase: Reversed Phase,</li><li>Pore Size: 100 Å</li><li>pH: 2 to 8</li></ul><h4>Syncronis HPLC and UHPLC Columns</h4><h5>Enter a new world of chromatography</h5>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.<h5>Features of Syncronis columns</h5>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:<ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Syncronis PREP HILIC HPLC Columns

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes.

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations.&nbsp;The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. Our columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.<h5>Alternative Selectivity</h5><ul><li>Zwitterionic bonded phase enhances retention of polar and hydrophilic analytes.</li><li>No need for ion-pair or derivatization.</li><li>Enhanced sensitivity with MS detection.</li><li>Highly organic mobile phase facilitates desolvation in ESI-MS</li></ul>Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.<h5>Standard Threaded and Standard Flange Hardware</h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5>XtendedLife Hardware</h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.<h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Endcapped: No</li><li>Max. Pressure: 2000 psi (140 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Zwitterionic</li><li>Surface Area: 320 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 5%</li><li>Phase: HILIC</li><li>Pore Size: 100 Å</li><li>pH: 2 to 8</li></ul><h4>Syncronis HPLC and UHPLC Columns</h4><h5>Enter a new world of chromatography</h5>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.<h5>Features of Syncronis columns</h5>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:<ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Products from Thermo Fisher Scientific - page 7

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