Ultrashort chain per- and polyfluoroalkyl substances (USC-PFAS),defined as those with three or fewer carbon atoms, are characterisedby their significant mobility within the environment, high water solubility,and low affinity for organic matter. These properties contributeto their widespread presence in environmental waters and wastewaters,as well as drinking waters. The environmental concerns associatedwith USC-PFAS include their persistence, potential for groundwatercontamination, and challenges in remediation due to their resistance to conventional treatment methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 1: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg 245w," sizes="100vw" width="602" height="247"><h4>Occurence, uses and sources</h4>Ultrashort chain PFAS have been poorly characterised compared with their longer chain counterparts, and understanding of their intended and unintended transformation processes is limited. Sources of USCPFAS include degradation of precursor compounds, atmospheric degradation of hydrofluorocarbons and hydrochlorofluorocarbons used as refrigerants, and intentional direct use in products like batteries. Crucially, TFA, a major ultrashort-chain PFAS, is also formed through the environmental breakdown of fluorinated pesticides and pharmaceuticals. They are also byproducts from historical electrochemical fluorination (ECF) manufacture of longer chain PFAS. USC-PFAS are also found in urban and industrial waste, as well as aqueous film-forming foams (AFFFs) used in firefighting.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 2: PFSA and PFCA categorization based on carbon chain length – ultrashort-chain, short-chain and long-chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg 245w," sizes="100vw" width="1496" height="629"><h4>Fate and transport</h4>Carbon chain length plays a key role in determining the physicochemical characteristics of different PFAS chemicals. Long chain PFAS for instance are more hydrophobic, a property that has been leveraged by removal techniques using carbonaceous materials like granular activated carbon (GAC). By contrast, short and ultrashort chain PFAS are hydrophilic, with characteristically low pKa values (i.e. more acidic), high water solubilities and decreased log Koc values (i.e. less adsorptive to organic carbon). These attributes mean that USC-PFAS dissociate freely in aqueous environments and are less prone to sorption onto natural solids. The outcome is that USCPFAS are highly mobile in the aquatic environment and can travel far from contamination source areas.<h4>The global regulatory landscape</h4>Awareness about the persistence and bioaccumulative nature of C8-C14 PFAS began to attract widespread concern near the turn of the millennium, and inclusion of PFOS, PFOA and PFHxS on the persistent organic pollutants (POPs) list of the Stockholm Convention soon ensued. Consequentially, manufacturers began adopting short and ultrashort chain alternatives.However, with the change from C8 to C4 and shorter fluorinated carbon chains came the sacrifice to lower technical performance, and greater quantities were needed for equivalent performance. Hence, while it is generally accepted that short and ultrashort chain PFAS have lower toxicological profiles, environmental risks are not necessarily obviated since USC-PFAS presence, and continuous, cumulative, release suggests that adverse risks may lie ahead.Global regulations of USC-PFAS vary by region and are often included under broader PFAS regulations. For example, the European Union has specific guidelines and limits for the total concentration of PFAS compounds in drinking water (as set out in EU Directive 2020/2184). The EU's Drinking Water Directive established a limit of 0.5 μg/L for 'PFAS Total,' although ongoing discussions concern the specific methodology for incorporating trifluoroacetic acid (TFA). Furthermore, TFA was recently added to the priority list for the initial proposed sum of 24 PFAS in surface water. <a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/09/23/water-pollution-council-and-parliament-reach-provisional-deal-to-update-priority-substances-in-surface-and-ground-waters/">LINK</a><h4>PFAS sampling containers</h4>Only tested and verified sampling containers are recommended to ensure reliable results in PFAS analysis; these containers are available upon request from ALS laboratories.<h4>Targeted analytical services are becoming more accessible</h4>Testing for USC-PFAS is challenging due to several analytical factors. Background contamination and matrix noise can interfere with the detection of these compounds, particularly trifluoroacetic acid (TFA). Furthermore, sample preparation presents a significant challenge because of the constant risk of cross-contamination. To mitigate this risk, the sample preparation procedure was specifically designed to be as simple and straightforward as possible.Other USC-PFAS compounds generally have lower ambient background concentrations, which makes achieving lower Limits of Reporting (LOR) more readily achievable. ALS has successfully developed a testing method that includes USC-PFAS compounds in environmental water matrices, achieving a total LOR of 50 ng/L for TFA and even lower LORs for other USC compounds. The full list of analyzed USC-PFAS is summarized in table 1:<h5>USC-PFAS Compounds / CAS No.</h5><ul><li>Trifluoroacetic acid (TFA), Perfluoroethanoic acid (PFEtA): 76-05-1</li><li>Trifluoromethane sulfonic acid (TFMS), Perfluoromethane sulfonic acid (PFMeS): 1493-13-6</li><li>Perfluoroethane sulfonic acid (PFEtS): 354-88-1</li><li>Perfluoropropanoic acid (PFPrA): 422-64-0</li><li>Perfluoropropane sulfonic acid (PFPrS): 423-41-6</li><li>Trifluoromethane sulfinic acid: 34642-42-7</li><li>Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI): 90076-65-6</li></ul>&nbsp;

EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources

<h4>Why Consider HILIC in Modern Oligonucleotide Analysis?</h4>Oligonucleotides play a central role in gene therapies, molecular diagnostics, and cutting-edge biomedical research. Before these molecules reach application, however, they must be carefully characterized to confirm purity, identity, and performance.Analyzing therapeutic oligonucleotides is far from straightforward. Their polarity, structural diversity, and modifications demand highly selective and MS-compatible separation methods. Although ion-pair reversed-phase LC (IP-RP-LC) remains the established standard for LC-MS workflows, hydrophilic interaction liquid chromatography (HILIC) is rapidly emerging as a powerful complementary technique.When IP-RP approaches reach practical or performance limits, HILIC offers an attractive alternative, delivering strong retention and efficient separation of highly polar oligonucleotides — without relying on ion-pairing reagents.<h4>How Does HILIC Separate Oligonucleotides?</h4>HILIC essentially reverses conventional reversed-phase logic. It combines:<ul><li>A polar stationary phase</li><li>An organic-rich mobile phase</li></ul>In this configuration, the stationary phase retains a thin, water-enriched layer on its surface. Polar analytes — such as oligonucleotides — partition into this aqueous layer while the acetonitrile-rich mobile phase passes through.This mechanism allows:<ul><li>Retention of short or highly polar oligonucleotides</li><li>Separation of modified sequences</li><li>LC-MS compatibility without ion-pairing reagents</li></ul>The key to performance lies in carefully tuning the parameters that govern HILIC selectivity and retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp" alt="KNAUER: Figure 1 - Key Parameter of HILIC Mode." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp 245w," sizes="100vw" width="945" height="433"><h4>What Controls Retention in HILIC?</h4>Several interaction mechanisms contribute to analyte behavior in HILIC:<h5>Hydrophilic Partitioning – The Primary Mechanism</h5>Polar molecules distribute into the thin water layer formed on the stationary phase surface. The greater the polarity of the analyte, the stronger its affinity for this layer, leading to increased retention.<h5>Hydrogen Bonding – Fine-Tuning Effects</h5>Functional groups such as –OH, –NH₂, and –COOH can form hydrogen bonds with the stationary phase, subtly influencing retention time and selectivity.<h5>Electrostatic Contributions – The Role of Charge</h5>Some HILIC materials (e.g., bare silica) exhibit surface charges. Depending on mobile phase pH and buffer composition, electrostatic interactions can either enhance or suppress analyte retention.<h5>Dipole and Ion–Dipole Interactions – Added Selectivity</h5>Highly polar or zwitterionic compounds — including phosphorylated species and sugars — may engage in dipole–dipole or ion–dipole interactions, further refining separation behavior.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp" alt="KNAUER: Figure 2 - Hydrophilic Partitioning on Water-enriched Layer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp 245w," sizes="100vw" width="945" height="409"><h4>How to Develop a HILIC Method for Oligonucleotides</h4>Like any chromatographic method, successful HILIC development follows a structured approach:<h5>Step 1: Define the Analytes</h5>Understand oligonucleotide length, polarity, charge, and modifications.<h5>Step 2: Select a Suitable Column</h5>Choose a polar stationary phase (e.g., amide or zwitterionic) appropriate for the oligonucleotide’s chemical properties.<h5>Step 3: Optimize the Mobile Phase</h5><ul><li>Start with 70–95% acetonitrile</li><li>Use a volatile buffer (e.g., ammonium formate or ammonium acetate, typically 5–20 mM)</li><li>Adjust pH (commonly between 6–8, with testing up to pH 9 if column stability allows)</li></ul>Small pH shifts can significantly alter selectivity and MS response.<h5>Step 4: Design the Gradient</h5>Employ a gradient that increases water content to modulate retention and separate oligonucleotides by size or chemical modification.<h5>Step 5: Match Injection Conditions</h5>Samples should be injected in a solvent similar to the starting mobile phase (high organic content) to prevent peak distortion.<h5>Step 6: Evaluate Performance</h5>Assess:<ul><li>Resolution</li><li>Retention time stability</li><li>Peak shape</li><li>Reproducibility</li></ul>Balancing separation performance with MS ionization efficiency is essential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp" alt="KNAUER: Table 1 - HILIC Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp 245w," sizes="100vw" width="945" height="357"><h4>Practical Tips for High-Performance HILIC</h4><h5>Maintain a Stable Water Layer</h5>A minimum of ~5% water is necessary at the start of the run. Too little water can eliminate retention entirely. Adjust water content carefully to fine-tune retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp" alt="KNAUER: Figure 3 - Water Adjustment Principle for HILIC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp 245w," sizes="100vw" width="951" height="374"><h5>Use High Organic Content</h5>HILIC typically operates best with 70–95% acetonitrile. Polar analytes are retained more effectively under these conditions.<h5>Include a Volatile Buffer</h5>Use low concentrations (5–20 mM) of ammonium formate or acetate to:<ul><li>Stabilize pH</li><li>Reduce peak tailing</li><li>Maintain MS compatibility</li></ul><h5>Control Injection Solvent</h5>Avoid high-water sample solvents. They disrupt the partitioning mechanism and distort peaks.<h5>Allow Proper Column Equilibration</h5>HILIC columns require longer equilibration times than reversed-phase columns — typically 10–20 column volumes after major gradient or solvent changes.<h5>Consider Temperature</h5>Operating at 30–50 °C can:<ul><li>Improve peak shape</li><li>Reduce mobile phase viscosity</li><li>Stabilize retention behavior</li></ul><h4>From Alternative to Essential? Final Perspective</h4>HILIC-MS is steadily advancing as a strong, ion-pair-free option for oligonucleotide analysis. While IP-RP-LC still dominates in sensitivity for many applications, HILIC is closing the gap and offering valuable flexibility — particularly for highly polar or challenging sequences.Although HILIC follows different chromatographic principles than reversed-phase separations, once optimized, it can deliver robust and highly selective results.Rather than being a niche method, HILIC is becoming a strategic tool in modern oligonucleotide workflows — especially when MS compatibility and method versatility are priorities.If you are developing or refining an oligonucleotide LC-MS method, HILIC may be worth integrating into your analytical toolbox.<blockquote>For additional information or discussion, feel free to contact: <a>sales@knauer.net<svg><use></use></svg></a>For direct scientific inquiries, reach out to the author at: <a>kindler@knauer.net<svg><use></use></svg></a></blockquote>Stay tuned for more insights in the “Oligos Made Easy” series.

Oligos Made Easy - Part 4: HILIC

News from LabRulezLCMS Library - Week 09, 2026

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For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

ALS Czech Republic

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

The Analytical Scientist

Agilent Technologies

Technology Networks

Bruker

Phenomenex

Waters Corporation

PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns</h4>
<ul>
<li>Recognize columns exhibiting comparable selectivity to your current column selection</li>
<li>Identify columns with different or orthogonal selectivity to your current column of choice</li>
<li>Understand the resources that are available for selectivity data for HPLC columns</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Recognize columns exhibiting comparable selectivity to your current column selection.

Identification of Equivalent and Orthogonal HPLC Columns

The CLIA proficiency testing revisions will take effect on July 11, 2024, introducing significant changes to both microbiology and non-microbiology laboratory sections. These updates include modifications to grading, reporting and the regulation of certain analytes.
Join Dr. Bremansu Osa-Andrews from the University of Florida for an in-depth webinar where he will elucidate the details of the CLIA 2024 final rule, helping laboratories stay informed and prepared for future inspections.
In this comprehensive session, Dr. Osa-Andrews will:
<ul>
<li>Summarize the CLIA Proficiency Testing Final Rule&#39;s historical aspects and other provisions</li>
<li>Describe the finalized requirements, including the microbiology changes and the non-microbiology changes, total allowable error and other acceptable limits</li>
<li>Explain some applicable steps laboratorians to prepare for the upcoming CLIA regulatory changes</li>
</ul>
<blockquote>
Presenter: Bremansu Osa-Andrews, PhD (Medical Director and Clinical Assistant Professor of Pathology, University of Florida)
</blockquote>

Technology Networks: What the CLIA 2024 Final Rule Means for Your Laboratory

What the CLIA 2024 Final Rule Means for Your Laboratory

Elucidating the details of the CLIA 2024 final rule, helping laboratories stay informed and prepared for future inspections.

Immune cell activation, differentiation and function are metabolically demanding processes, and it has now become clear that nutrient access and metabolism play a major role in regulating lymphocyte fate and function. These axes of regulation are crucially important in shaping the immune system when it is activating in metabolically demanding environments, such as tumor tissue. Indeed, the immune response to cancer is characterized by several metabolic defects.
In this webinar, Dr. Delgoffe will discuss some of these metabolic insufficiencies within the T cell compartment of solid tumors and reveal novel insights into how these metabolic nodes may be modulated to improve the immune response to cancer.
Attend this webinar to learn:
<ul>
<li>How T cells are sensitive to metabolites</li>
<li>The metabolic programs enacted by T cells as they become exhausted</li>
<li>How immunometabolism may be leveraged for cancer therapy</li>
</ul>
<blockquote>
Presenter: Dr. Greg M. Delgoffe (Associate Professor of Immunology &amp; Director of the Tumor Microenvironment Center, University of Pittsburgh &amp; UPMC Hillman Cancer Center)
</blockquote>

Technology Networks: Immunometabolic Features of Cancer Immunity

Immunometabolic Features of Cancer Immunity

Discussion of metabolic insufficiencies within the T cell compartment of solid tumors and reveal how these metabolic nodes may be modulated.

Join us for an overview of a powerful software solution specifically designed for scientists, offering a wide range of features and functionalities to meet the diverse needs of modern laboratories. From data management to instrument integration and advanced analytics, this software provides a comprehensive solution for laboratory automation and optimization.
During this webinar, we will highlight numerous advantages of the platform, including:
<ul>
<li>Enhanced lab efficiency: Secure, centralized data management that features automated data transfer, built-in backup and archiving capabilities, audit trails and user and role access control.</li>
<li>Easy collaboration: Unhindered web-based access facilitates efficient teamwork and data sharing from anywhere, at any time.</li>
<li>Improved instrument utilization: Oversee operations with real-time instrument status and ensure effective instrument management and utilization with digital logbooks and online scheduler. </li>
<li>Increased functionality: Explore the added functionality delivered by the Thermo Scientific Ardia Platform to other Thermo Fisher Scientific software applications including, Xcalibur, Chromeleon Chromatography Data System, BioPharma Finder, Proteome Discoverer and Compound Discoverer.</li>
<li>Greater flexibility: Discover the integration of this platform with third party software, e.g., Skyline and Spectronaut, using automated data transfer.</li>
</ul>
Unlock the potential to expand your lab&#39;s capabilities and achieve extraordinary results. Join this webinar and help us understand your journey with time for questions and participation in discussions. We look forward to seeing you there! 
Webinar Learning Objectives:
<ul>
<li>Acquire a comprehensive understanding of the Thermo Scientific Ardia Platform and its potential to transform your laboratory operations.</li>
<li>Discover the benefits of a secure, centralized data repository and management system that enables collaboration.</li>
<li>Learn how laboratory assets can be automated and managed more efficiently to optimize laboratory operations.</li>
<li>Gain actionable knowledge on how to leverage software to optimize workflows, improve data management, and drive scientific discoveries.</li>
</ul>
<blockquote>
Presenter: Mark Sanders (Director, Business Development &amp; Partner Management, Chromatography &amp; Mass Spectrometry Software, Thermo Fisher Scientific)
</blockquote>
<blockquote>
With a wealth of experience in the field of chromatography and mass spectrometry software, Mark Sanders currently serves as the Director of Business Development &amp; Partner Management for Chromatography &amp; Mass Spectrometry Software at Thermo Fisher Scientific, where he plays a pivotal role in driving the strategic growth and development of software solutions in this domain. Prior to his current role, Mark led the software product management team for Life Sciences Mass Spectrometry Software at Thermo Fisher Scientific. In this capacity, he was responsible for overseeing the software portfolio in crucial areas such as metabolomics/lipidomics, small molecule structure elucidation, bioanalytical quantitation, metabolism, proteomics, and protein characterization. Before joining Thermo Fisher Scientific in 2008, Mark held the position of Senior Principal Scientist and Leader of the Discovery Analytical Sciences Mass Spectrometry Group at Bristol-Myers Squibb in Princeton, NJ. In this role, he led a team of experts in the field of mass spectrometry, driving innovation and advancing analytical capabilities for drug discovery and development.
</blockquote>

The Analytical Scientist: Unlock the power of connectivity: Enhancing collaboration and data sharing in chromatography and mass spectrometry analysis

Unlock the power of connectivity: Enhancing collaboration and data sharing in chromatography and mass spectrometry analysis

Overview of a powerful software solution specifically designed for scientists.

Collaboration and data sharing in chromatography and MS

<h4>Event Overview:</h4>
In this webinar, we present an mRNA characterization workflow that includes sample preparation, LC-MS methodology and bioinformatics processing for the evaluation of 5&#39; Cap efficiency, mapping of oligonucleotide sequence fragments, isomer resolution and Poly (A) tail intact mass analysis.
Who Should Attend:
<ul>
<li>Researchers and Scientists: Ideal for those seeking to expand their insights into LC-MS characterization techniques and applications of mRNA modifications.</li>
<li>Quality Control and In-Process Testing Professionals: Recommended for professionals involved in quality control, in-process testing, or analytical development.</li>
<li>Biopharmaceutical Industry Professionals: Valuable for those evaluating and optimizing their current mRNA-based therapeutics development and characterization solutions.</li>
</ul>
Key Learning Points:
<ul>
<li>Explore the LC-MS characterization method for 5&#39; Cap, coding sequence mapping, and Poly(A) tail analysis.</li>
<li>Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances.</li>
<li>Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification.</li>
<li>Review of bioinformatics processing workflow</li>
</ul>
<blockquote>
Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
</blockquote>

Phenomenex: mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

Presentation of mRNA characterization workflow including sample preparation, LC-MS methodology and bioinformatics processing.

mRNA Characterization: What IPRP–MS Can Tell You

Psychedelics have captivated both scientists and the general public, offering groundbreaking insights into mental health and consciousness. For chemists, these compounds present a fascinating area of study, revealing intricate chemical structures and mechanisms of action.
Technology Networks is excited to offer an exclusive Ask Me Anything (AMA) session with Andrew Wood, a distinguished chemist renowned for his extensive research on psychedelics. His work has greatly enhanced our understanding of the chemical properties and therapeutic potential of substances like psilocybin.
What to Expect:
In this interactive Q&amp;A session, Andrew Wood will explore the complexities of psilocybin, answering your questions and discussing crucial topics such as:
<ul>
<li>Analytical Approaches for Psilocybin: Discover the sophisticated methods used to analyse and characterize psilocybin, ensuring its purity and potency.</li>
<li>Chemical Structure and Properties of Psilocybin: Gain insights into the molecular composition of psilocybin and how its chemical properties influence its effects.</li>
</ul>
This free-to-attend event allows you to ask Andrew any question during our live virtual session. We highly encourage you to join us live to have your questions answered in real-time. 
Whether you&#39;re a seasoned researcher, a student of chemistry, or simply curious about psychedelics, this AMA offers valuable insights and a unique opportunity to engage directly with an expert in the field.
<blockquote>
Presenter: Andrew Wood (Research and Development Scientist, Apex)
</blockquote>

Technology Networks: Ask Me Anything: The Chemistry of Psilocybin

Ask Me Anything: The Chemistry of Psilocybin

Discover the sophisticated methods used to analyse and characterize psilocybin, ensuring its purity and potency.

Recent instrumental breakthroughs in mass spectrometry are a real game-changer and have opened new possibilities for proteomic analysis implying extreme sensitivity at high throughput.
On the one hand single cell proteome analysis has become feasible: we have pushed a timsTOF Ultra instrument to its limits to reach a proteome coverage of around 5.000 proteins identified in a single cell. On the other hand, chromatography and MS acquisition parameters optimizations combined with innovative AI-based data interpretation have enabled us to reach unprecedented coverage in immunopeptidomics with close to 20.000 immunopeptides identified from limited numbers of cells. We will show how fine application-specific optimizations of every step of the proteomics workflow are strongly beneficial to the output results.
<blockquote>
Presenter: Christine Carapito (CNRS Research Director, Co-head of the BioOrganic Mass Spectrometry Laboratory, CNRS and University of Strasbourg, France)
</blockquote>
<blockquote>
Presenter: Jeewan Babu Rijal (Post-doctoral fellow at the BioOrganic Mass Spectrometry Laboratory, CNRS and University of Strasbourg, France)
</blockquote>

Bruker: timsTOF Ultra: Immunopeptidomics and single cell proteomics explorations

timsTOF Ultra: Immunopeptidomics and single cell proteomics explorations

Fine application-specific optimizations of every step of the proteomics workflow are strongly beneficial to the output results.

timsTOF Ultra: Immunopeptidomics and proteomics

<h4>Automating Laboratory Workflows with Andrew+ and OneLab Software: A Virtual Demonstration</h4>
We are excited to invite you to our upcoming webinar series on how to easily bring automation to your lab and transform your current workflows.
<h4>EPISODE 2 - Pharma Workflows</h4>
In this episode, we’re excited to show off Andrew+, an advanced pipetting robot, and OneLab, a workflow design execution software, two game changers in pharmaceutical workflows. They use automation to streamline everything from drug discovery to quality control.
In this episode, you will:
<ul>
<li>Hear directly from Maria Maher, from Regeneron Ireland, how she successfully increased the overall lab efficiency by implementing the Andrew+ pipetting robot to multistep sample preparation workflows.</li>
<li>Take a tour of the user-friendly OneLab Software, showing how Andrew+ can be intuitively guided to carry out complex tasks. This reduces manual labor and minimizes errors.</li>
</ul>
Andrew+ and OneLab can automate sample preparation protocols, including:
<ul>
<li>General liquid handling</li>
<li>Serial dilutions</li>
<li>Concentration normalizations</li>
<li>And even more complex workflows involving filtration, sample extraction or magnetic beads.</li>
</ul>
These steps are crucial for ensuring the accuracy and reliability of subsequent analytical procedures.
We’ll also talk about data management capabilities, highlighting how OneLab ensures data integrity and helps meet regulatory standards.
So, come join us for this demonstration!
Not sure to be able to attend live sessions?
Register today and watch the webinars on-demand
<h4>Other episodes rfom the series:</h4>
Transform Your Daily Lab Operations with Automation - Episode 1: Biomolecules workflows
<ul>
<li>Tuesday, June 11, 2024, 11:00 AM CEST</li>
</ul>
Transform Your Daily Lab Operations with Automation - Episode 3: Food and Environmental Workflows
<ul>
<li>Thursday, September 19, 2024, 11:00 AM CEST</li>
</ul>
<blockquote>
Presenter: Maria Maher (Process Scientist, Regeneron Ireland)
</blockquote>
<blockquote>
Maria Maher is a Process Scientist in the Process Sciences Department in Regeneron Ireland. She jhas worked with the Andrew+ pipetting robot to incorporate multistep sample preparation workflows that involve multiple sample manipulation steps in an effort to reduce the overall variability of the results achieved, minimise analyst input and increase the overall lab efficiency.
</blockquote>
<blockquote>
Presenter: Jasper Eaton (Senior Product Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Jasper has a Bachelor’s degree in General Biology and obtained a Masters Degree in Molecular and Cellular Biology from the University of Grenoble-Alpes. He was previously the EU Sales Manager at Andrew Alliance since 2017, and is now a Senior Product Marketing Manager at Waters Corporation. His experience has made him well versed in the challenges faced by customers looking to automate their lab workflows.
</blockquote>
<blockquote>
Presenter: Tobias Langrock (Senior Application Specialist Chemistry, Europe, Waters Corporation)
</blockquote>
<blockquote>
During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.
</blockquote>

Waters Corporation: Transform Your Daily Lab Operations with Automation - Episode 2: Pharma workflows

Transform Your Daily Lab Operations with Automation - Episode 2: Pharma workflows

 Showing off Andrew+, an advanced pipetting robot, and OneLab, a workflow design execution software, two game changers in pharmaceutical workflows.

Automation with Andrew+ Episode 2: Pharma workflows

After the approval of the final version of ICH Q14, dealing with the Analytical method development and the new update of the ICH Q2, analytical validation, incorporating the AQbD concept to the analytical testing, it’s becoming difficult to generate results at the same pace as we have done historically. However, these changes give the developers a powerful tool that assures the ruggedness and reliability of the analytical method. In these webinars we are going to review with our partner Chromsword, the chance to incorporate the new requirements proposed in the quality guidelines in the LC method development workflow, along with the automation possibilities. Easing the analytical and statistical effort in an unprecedent manner.
During the first part of the seminar are going to review the regulatory landscape, and the available tools both from the software point of view and hardware able to automatize the whole process. Actually the combination of the right hardware and software leads to a significant reduction of development times, securing the method’s ruggedness during the process. A process which meets all requirements from ICH Q14 guidelines.
<blockquote>
Presenter: Antonio Chana (Product Specialist Liquid Phase Separation, Agilent Technologies)
</blockquote>
<blockquote>
Antonio Chana, Doctor of Chemical Sciences and product specialist in liquid chromatography. Antonio joined Agilent from the pharmaceutical industry, where he occupied the positions of analytical development manager, quality control manager and laboratory manager. Over the years he has participated in audits of quality and integrity of data with different national and international regulatory bodies.
</blockquote>
<blockquote>
Presenter: Arturs Kalimulins (Sales Director, ChromSword)
</blockquote>
<blockquote>
Arturs Kalimulins is the Sales Director at ChromSword, bringing over 15 years of industry experience to the table. Throughout his career, Arturs has collaborated with pharmaceutical companies worldwide to enhance the speed and efficiency of HPLC method development. His background in IT solutions has provided him with a unique perspective on typical HPLC challenges and workflows.
</blockquote>
<blockquote>
Currently, Arturs is leading the AQbD workflow management project at ChromSword. This initiative aims to develop and deliver tools to analytical laboratories that organize and systemize every step, task, and document in the HPLC method development process.
</blockquote>

Agilent Technologies - Analytical Method Development under ICH Q14

Analytical Method Development under ICH Q14 Part 1

We will review the chance to incorporate the new requirements proposed in the quality guidelines in the LC method development workflow.

After the approval of the final version of ICH Q14, dealing with the Analytical method development and the new update of the ICH Q2, analytical validation, incorporating the AQbD concept to the analytical testing, it’s becoming difficult to generate results at the same pace as we have done historically. However, these changes give the developers a powerful tool that assures the ruggedness and reliability of the analytical method.
In both webinars we will review, with our partner Chromsword, the chance to incorporate the new requirements proposed in the quality guidelines in the LC method development workflow, along with the automation possibilities. Easing the analytical and statistical effort in an unprecedent manner. 
During this webinar, the second in a two part series, we will share examples of use. Both from the real implementation in a pharmaceutical lab, and an example of complex development upon lipidic nanoparticles composition.
<blockquote>
Presenter: Dr. Aline Bayerle (Applikationsspezialistin für LC basierte Lösungen, Agilent Technologies)
</blockquote>
<blockquote>
Aline works as an application specialist for LC based solutions at Agilent. During 10+ years of working as an analytical chemist in both the pharmaceutical industry, a GLP/cGMP environment, and at Agilent, she was able to gain experience in a variety of applications.
</blockquote>

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