Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.<h4>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</h4>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.<div><div>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (Figure 1).Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493">While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.<h4>Key takeaways</h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</h4>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.<h4>Online SPE analysis provides key benefits like:</h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul>Let’s explore what this technique looks like in practice.<h4>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</h4>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"><h4>The process for analyzing a hops standard solution is straightforward:</h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"> on the SPE column</a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"> analytical column </a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (Figure 3).</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"><h4>Online SPE for analyzing α- and β-bitter acids in real hops samples</h4>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (Figure 4).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4>From carefully procured samples to tasty sips</h4>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.<ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf">check out our application note today</a>.<h4>FAQs</h4><div><div><h5>What are the key compounds in hops analyzed by HPLC?</h5>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</div><div><h5>What column types are recommended for hop acid separations?</h5>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</div><div><h5>What’s the best workflow for hops analysis?</h5>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</div></div><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#UHPLC #Automation</blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

Webinars LabRulezLCMS Week 27/2026

The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607">A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;<h4>Evaluation of measurement procedure performance&nbsp;</h4>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;<ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"><h4>Characterisation of candidate reference materials&nbsp;</h4>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;<ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4>Other ILCs&nbsp;</h4>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).

Interlaboratory comparisons other than proficiency testing

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Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
By attending this event you will:
<ul>
<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
</li>
<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
</li>
<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
</li>
<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
</li>
<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
</li>
</ul>
<h5>Talk 1: Agilent Tools and Technologies to Drive Advancements in Biopharmaceutical Drug Development</h5>
<ul>
<li>Presented by Dr. Hervé Chaulet, Molecular and Biological Research Market Specialist, Agilent Technologies</li>
</ul>
Discovering, developing, and manufacturing biopharmaceuticals requires you to stay abreast of advances in knowledge and improvements in technology while constantly navigating a maze of business and scientific challenges. Agilent can be a trusted partner providing expertise and end-to-end workflows including systems, software, consumables, and services for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics, and cell and gene therapies.
<h5>Talk 2: Quality Control In IVT mRNA Therapeutics and Vaccine Development Workflow Using The Agilent Fragment Analyzer System</h5>
<ul>
<li>Presented by Dr. Leslie Friedmann, Product Manager, Agilent Technologies</li>
</ul>
Reliable and robust analysis is essential throughout in vitro transcription (IVT) mRNA workflows, providing important quality information to help minimize risk and aid in the production of a consistent product. The Agilent Fragment Analyzer systems can be used for several QC steps at different checkpoints in mRNA vaccine development, providing accurate and precise sizing and integrity analysis. These QC steps are to determine the quality and size of the linearized plasmid, the size and purity of the IVT mRNA, length of the poly(A) tail, and purity of the final mRNA vaccine product.
<h5>Talk 3: Analysis of NIST mAb Reference Material by Parallel SDS-Capillary Electrophoresis</h5>
<ul>
<li>Presented by Dr. Kyle Luttgeharm, Product Manager, Agilent Technologies</li>
</ul>
Monoclonal antibody drug products are complex biomolecules that must be tested for critical quality attributes (CQAs) such as monomeric purity and percent glycosylation. Capillary gel electrophoresis methods have been adopted as the standard method for size heterogeneity characterization, however legacy capillary electrophoresis (CE) methods involve complicated workflows or are cost prohibitive. To address these problems with legacy methods, Agilent has developed a 12-channel, parallel capillary electrophoresis-SDS (CE-SDS) instrument and reagents. The system uses a high-resolution sieving gel, allowing for resolution of mAb species including the non-glycosylated heavy chain and light chain. To demonstrate the capabilities of the ProteoAnalyzer, the NIST mAb was characterized under both reduced and non-reduced conditions and compared to the NIST data sheet.
<h5>Talk 4: Advanced Analytics for Cell Therapy Research, Process Development, and Manufacturing</h5>
<ul>
<li>Presented by Dr. Kelly Kroeger, Science &amp; Technology Advisor, Agilent Technologies</li>
</ul>
With immunology and immune-based therapies rising as a pillar of science and therapeutics, we are seeing a foundational shift from targeting specific proteins and pathways to actually targeting the immune cellular network that resolves and prevents disease. Therefore there is a critical need for cell analysis tools to facilitate understanding of the dynamic nature of the interaction of these cellular networks whether it is in the context of immune cells or immune cells with cancer cells.
To address these new challenges, we at Agilent Technologies have developed a portfolio of cell analysis solutions that provide researchers with more relevant tools to measure living cells, dynamically track them, and model their microenvironments. These next-generation therapies require methods and technologies to enable assessment of real-time cell function, phenotype, and fate. Used together, the Agilent tool bench provides the capability to measure and develop immune cell function more effectively, thus enabling translational researchers and developers to achieve the necessary level of potential therapeutic potency and safety.

Technology Networks: Biopharma Innovations Summit

Biopharma Innovations Summit Day 1

Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development.

Biomonitoring of toxicants like pesticides and PFAS in humans is essential for understanding how chemical exposure affects our health, yet chemical coverage is currently limited with targeted LC-MS approaches.Untargeted data acquisition, however, is emerging as a promising tool for broader chemical coverage and understanding the possible outcomes of chemical exposure on human health.In this webinar, our expert speaker will discuss how a new method with sequential acquisition of targeted and untargeted data can be used to analyze the exposome and the metabolome in a single LC-MS run, providing greater insights into the effects of chemical exposure.Attend this webinar to:<ul><li>Find out about the benefits of sequential targeted and untargeted data acquisition in a single LC-MS run</li><li>Understand how high sensitivity and broad chemical coverage can be achieved simultaneously for performing exposomic and metabolomic experiments</li><li>Discover a novel method that can be tailored for many different applications in the field of small molecule omics</li></ul><blockquote>Presenter: Dr. Vinicius Verri Hernandes (Postdoctoral Researcher at University of Vienna)</blockquote><blockquote>Vinicius obtained his degree in Chemistry followed by a Master in Analytical Chemistry in 2013 from the University of Campinas in Brazil, with focus on mass spectrometry. He later started a joint PhD program between the same university and the Centre for Excellence in Metabolomics in Bioanalysis in Madrid, Spain, working on multiplatform metabolomics on dried blood spots. From 2018 to 2022 he worked as a postdoctoral researcher at the European Research Academy (EURAC) in Bolzano, Italy, being responsible for targeted and untargeted data generation for 5000 samples from the CHRIS populational study. Currently at the University of Vienna, Vinicius focus his work on the combination of targeted and untargeted approaches for exposomics and metabolomics, as well as in the development of analytical workflows for exposure analysis on dried blood spots.</blockquote>

Technology Networks: Bridging Exposomics and Metabolomics in a Single LC-MS Run

Bridging Exposomics and Metabolomics in a Single LC-MS Run

How a new method with sequential acquisition of targeted and untargeted data can be used to analyze the exposome and the metabolome in a single LC-MS run.

Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
By attending this event you will:
<ul>
<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
</li>
<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
</li>
<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
</li>
<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
</li>
<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
</li>
</ul>
<h5>Talk 1: Advanced Analytical Solutions for Nucleic Acid Analysis and Purification</h5>
<ul>
<li>Presented by Dr. Charlotte Lekieffre, Biopharma Application Specialist, Agilent Technologies</li>
</ul>
The emphasis during the second day of the Summit will be on advanced analytical solutions, such as HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Additionally, you’ll discover techniques to enhance the efficiency and accuracy of your processes by scaling up oligonucleotide purification using Agilent biocolumns. 
<h5>Talk 2: Extracted Yeast tRNA Sequencing by LC-MS/MS</h5>
<ul>
<li>Presented by Aurore Attina, Engineer of Mass Spectrometry, University of Montpellier</li>
</ul>
RNA modifications are essential for RNA stability, maturation, and functionality; directly influencing protein translation and gene regulation. They play a central role in various biological processes and are associated with many diseases, including cancers and neurological disorders. tRNA (transfer RNA), which plays a key role in protein synthesis, is the most modified molecule in the organism known to date. tRNA modifications are essential to ensure their correct function in protein translation, maintain the accuracy and fidelity of the genetic code, and enable flexible adaptation to changing cellular conditions and are therefore a crucial attribute to monitor. To monitor and map these modifications of tRNA, we have developed a LC-HRMS analytical workflow, first using synthetic oligonucleotide for the method optimization. In this webinar, we will first go through the various steps involved in LC-MS/MS method optimization using synthetic oligonucleotides. In a second part, we will present the protocol development of an enzymatic digestion applied to tRNA extracted from yeast. This digestion generates oligonucleotide fragments that can be analyzed by LC-MS/MS for sequence confirmation.
<h5>Talk 3: Discover Agilent’s Software Tools to Confirm Oligonucleotide Sequence Integrity</h5>
<ul>
<li>Presented by Dr. Holger Stalz, LC/MS Product Specialist Austria &amp; Switzerland, Agilent Technologies</li>
</ul>
Mass spectrometry (MS) is increasingly important for the development of therapeutic modalities such as oligonucleotides. A common analytical method for large molecules is intact mass by LC/MS. This can be used for the characterization and identification of a product or process-related impurities, or simply as a confirmation of molecular weight. The newly introduced Agilent OpenLab CDS 2.8 SW version comes with a set of features to enable the automatization of biopharma workflows. If you want to know how LC/MSD Spectral Deconvolution of your biopharma samples can be automated, we will provide answers during our presentation. Even more details on Oligonucleotide sequence confirmation and impurities characterization can be acquired using the power of a high-resolution accurate mass system (HRAM) on the Agilent 6545XT AdvanceBio LC/Q-TOF. Analyzing the sample data with the software workflows in Agilent BioConfirm version 12 will support the user to get the trusted answers to their most relevant questions.
<h5>Talk 4 Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns</h5>
<ul>
<li>Presented by Dr. Andrea Tripodi, Application Development Engineer, Agilent Technologies</li>
</ul>
Several classes of nucleic acids, such as antisense oligonucleotides, small interfering RNAs (siRNAs), and aptamers are being investigated for potential therapeutic applications. Synthetic oligonucleotides inevitably contain many impurities due to limitations in the efficiency of each chemical reaction used during manufacture. They include deletion sequences (missing one or more nucleotides) and reaction by-products often arising during the final deprotection or cleavage steps. Ion pair reversed-phase (IP-RP) chromatography stands out as the prevalent method for analyzing and purifying oligonucleotides. For increased resolution and separation of your full-length synthetic oligonucleotide from closely related impurities, AdvanceBio Oligonucleotide is the ideal choice. The latest developments include semi-preparative, and lab-scale preparative columns packed with superficially porous particles that provide excellent performance and resolution without compromising column loading. A fast and easy scale-up method from analytical to semi-preparative scale was successfully achieved using a 10 mm ID column enabling higher sample loading using the same analytical LC instrument.

Biopharma Innovations Summit Day 2

Day one: Innovation and data insights<ul><li>Discover how to achieve high sensitivity, speed, and resilience when working with large, complex sample sets</li><li>Learn strategies to speed up your covalent protein drug discovery</li><li>Gain insights into accelerating the development of multiple modalities</li><li>Understand the latest advancements in quantitative proteomics with ZT Scan DIA</li><li>Overcome limitations with cutting-edge artificial intelligence quantitation software</li></ul>Agenda-at-a-glanceWelcome and introduction to innovation and data insights&nbsp;&nbsp;&nbsp;&nbsp;Advancing robustness and serviceability of a high-end triple quad mass spectrometer in a high-throughput bioanalytical lab<ul><li>Highly sensitive mass spectrometers allow the advanced bioanalytical labs of today to achieve the LOQ required of potent biologic drugs and provide maximum flexibility in choice of sample prep techniques. However robust mass spectrometers are crucial for several reasons:<ul><li>Aid in ensuring the consistent quantitative performance of an assay</li><li>Effectively allow a lab to meet their project timelines</li><li>Are essential for handling the inherent variability of analyzing diverse molecules and diverse samples required for bioanalysis</li></ul></li></ul>The new fast in drug discovery: a new MS system to study covalent protein drugs<ul><li>The combination of a super-fast sampling interface with the highly sensitive ZenoTOF 7600 system makes it an ideal system for evaluating covalent binding screens. Main findings in our experiments using the Echo® MS+ system:<ul><li>Speed: measurements from a full 384-well plate in 15 min or 1536-well plate in less than one hour</li><li>Precision: low injection volume supports repetitive measurements and time series; capture full kinetic data set in one experiment</li><li>Quality: high data quality allows for full automated processing, delivering immediate hitlist output</li></ul></li></ul>icIEF-UV/MS characterization of charge Isoforms for biotherapeutic products<ul><li>Characterization of icIEF charge isoforms, whether in early product development or associated with licensure filings, is usually achieved through ion exchange (IEX) liquid chromatography coupled with mass spectrometry. icIEF-MS technology has recently emerged to enable the direct characterization of specific species in the icIEF electropherogram, eliminating the need for extensive method development.<ul><li>Discover opportunities to advance core platform technologies with novel characterization approaches</li><li>Hear case studies on understanding molecular structure information</li><li>New approaches to characterizing post-translational modifications of charge isoforms</li></ul></li></ul>Expanding qualitative and quantitative sensitivity for immunopeptidomics using ZT Scan DIA<ul><li>Learn how utilizing a research-grade Zeno-trap time-of-flight LC-MS system can advance current applications in immunopeptidomics and proteomics, including:<ul><li>What is ZT Scan DIA, the technology and how it benefits data acquisition</li><li>Preliminary proteomics and immunopeptidomics results using ZT Scan DIA</li><li>Future applications of immunopeptidomics using ZT Scan DIA</li></ul></li></ul>Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysi<ul><li>High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:<ul><li>A molecular structure-dependent workflow</li><li>The holistic and user-independent processesing of HRMS data</li><li>The automatic quantification of small drug-like molecules</li></ul></li></ul>Q&amp;A roundtable: innovation and data insights with the experts<ul><li>Moderated by: Jamie Wighton, Senior Director LC-MS Research, SCIEX</li><li>Panelists include:<ul><li>Rathna Veeramachaneni, Director, Biopharma LC-MS/MS, KCAS Bio</li><li>Xiaping He, Senior Scientist, Pfizer</li><li>Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical Research</li><li>Tony Purcell, Professor, Monash University&nbsp;</li><li>Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert</li></ul></li></ul><blockquote>Moderator: Jamie Wighton (Senior Director LC-MS Research, SCIEX)Jamie joined SCIEX 20 years ago and has held multiple technical and leadership roles throughout his career at SCIEX. &nbsp;Starting out as an engineer in manufacturing operations, Jamie has since led teams in supply chain, product development, and most recently the research and product applications teams within R&amp;D. Prior to joining SCIEX, Jamie worked at JDS Uniphase as an optical test engineer.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Rathna Veeramachaneni (Director, Biopharma LC-MS/MS, KCAS Bioanalytical)Presenter: Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical ResearchPresenter: Xiaoping He, Senior Scientst, PfizerPresenter: Tony Purcell, Professor, Monash UniversityPresenter: Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert</blockquote>

SCIEX summit Day one: Innovation and data insights

Importance of LC-MS in food and environmental testing, pharmaceutical and biopharmaceutical discovery and development and biomedical and omics research.

Day two: Characterization and discovery<ul><li>Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics</li><li>Achieve in-depth profiling of metabolites and lipids in biological samples</li><li>Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)</li><li>Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches</li><li>Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to characterization and discoveryTop-down characterization of post-translational arginylation using electron activated dissociation (EAD)<ul><li>Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:<ul><li>Arginylation regulates CALR degradation and ER function</li><li>EAD is efficient in characterizing N-terminal arginylation</li><li>The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling</li></ul></li></ul>In-depth metabolomics and lipidomics analyses<ul><li>Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.<ul><li>Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies</li><li>Enhanced sensitivity enables deeper profiling of biological samples</li><li>Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)</li></ul></li></ul>MetID innovations in pharma: exploring new avenues for metabolite structure elucidationTime-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches<ul><li>Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:<ul><li>Overview of practical targeted and non-targeted approaches in food analysis</li><li>Application of a SWATH® approach to the analysis of pesticide residues in food</li><li>Application of IDA approaches in non-targeted food analyses</li></ul></li></ul>Reduce risk and accelerate cell line development with iCIEF-UV/MS technology<ul><li>Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability. &nbsp;<ul><li>How to utilize icIEF-UV/MS to identify sequence variants</li><li>How this information can be used to create a mitigation strategy</li><li>What more information in less time means to groups throughout process development</li></ul></li></ul>Q&amp;A roundtable: A deeper look at high-resolution mass spectrometry<ul><li>Moderated by: ose Castro-Perez, Vice President of Product Management, SCIEX</li><li>Panelists include:<ul><li>Tom Lin, Instructor, Washington University in St. Louis</li><li>Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich</li><li>Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería</li><li>Kirsten Nields, Principal Scientist, J&amp;J Innovative Medicines</li><li>Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</li></ul></li></ul><blockquote>Moderator: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEXTatjana is a Senior Product Manager at SCIEX, overseeing accurate mass systems since 2021. She collaborates with R&amp;D, sales, and marketing teams to ensure effective project execution and portfolio management.Previously, Tatjana was a Product Manager at MOBILion Systems, leading the pre-commercialization of SLIM-based ion mobility instruments. She also has held roles at Thermo Fisher Scientific as a Sr. Vertical Marketing Specialist and Sr. Field Applications Scientist.Tatjana holds a B.A. in chemistry from Austin College and a Ph.D. in biomedical sciences and biochemistry from the University of North Texas Health Science Center.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Tom Lin, Instructor, Washington University in St. LouisPresenter: Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz MunichPresenter: Francisco José Díaz-Galiano, Postdoctoral researcher, University of AlmeríaPresenter: Kirsten Nields, Principal Scientist, J&amp;J Innovative MedicinesPresenter: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</blockquote>

SCIEX summit Day two: Characterization and discovery

CIEX summit Day two: Characterization and discovery

Day three: Quantitation and screening<ul><li>Enhanced sensitivity and robustness deliver stringent quantitation for veterinary drug testing for meat</li><li>See how intuitive, compliant-ready SCIEX OS software enables pharmacokinetic parameter monitoring with ease and reliability</li><li>Understand the challenges and best practices for oligoneucleotide analysis by LC-MS&nbsp;</li><li>Discover how exploiting fast acquisition rates can be used to identify and quantify over 2250 fatty-acids lipids</li><li>Hear about the accurate detection and quantitation of trace-lelvel PFAS compounds</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to quantitation and screening&nbsp;&nbsp;&nbsp;&nbsp;Veterinary drug analysis: SCIEX 7500 system vs 7500+ system<ul><li>Biochemie Lab shares their expertise in veterinary drug analysis. With over 25 years of experience and strict adherence to Commission Regulation (EU) No 37/2010, Biochemie Lab processes more than 100,000 samples annually, providing comprehensive food and environmental testing.<ul><li>Learn how Biochemie Lab conducts veterinary drug testing with specialized veterinarians, covering a wide range of analyses, including residual veterinary drugs, anabolic substances, and microbiological tests.&nbsp;</li><li>Discover how they overcome challenges in analyzing complex animal-origin matrices like meat and offal using the SCIEX QTRAP 7500 system.</li><li>Explore their proficiency testing, method validation results, and ask questions directly from leading experts in the field.</li></ul></li></ul>Maximizing analytical potential and navigating changes in the validated laboratory with SCIEX OS software<ul><li>Having the right information available at every stage of the process is vital to bringing safe and effective therapies to market.. SCIEX OS software is a vital component of this process, from discovery to development. See how:<ul><li>Your pharma lab workflows can benefit from SCIEX OS software</li><li>Central Admin Console enables comprehensive audit configurations for full traceability</li><li>Professional services are available to assist in migrating from legacy Analyst software and change control support plansis</li></ul></li></ul>Approaches for the bioanalytical analysis of oligonucleotide therapies<ul><li>Oligonucleotide therapeutics are on the rise in drug development and regulatory submissions. These drugs face challenges with bioanalytical extraction and analytical detection due to their chemical backbone or other stability-enhancing modifications.<ul><li>Learn about bioanalytical challenges and best practices for oligonucleotide analysis</li><li>Hear case studies in the quantitation of oligonucleotide therapies using different mass spectrometry platforms</li><li>Gain insights into improved method development for obtaining validated assays for regulatory evaluations using high-resolution mass spectrometry in early-stage development</li></ul></li></ul>Fast scanning quantitative lipidomics analysis using the SCIEX 7500+ system<ul><li>A panel of &gt;2250 lipids was used to demonstrate the power and capability of the SCIEX 7500+ system to sensitively identify and quantify lipids at the fatty acid level using a multiple reaction monitoring (MRM). HILIC-like chromatography provides class separation to mitigate inter-lipid class isobaric overlap, but means that the speed at which individual MRM transitions are measured is crucial to ensure good coverage and quantitative accuracy.<ul><li>The ~4X faster analytical speed of the SCIEX 7500+ system improves the quantitative performance of a large, broadly targeted lipid panel</li><li>The number of lipid molecular species quantified with %CV &lt; 20 increased 59% during a period of the highest</li><li>MRM concurrency (n = 925) compared to the fastest speed recommended on the SCIEX 7500 system The faster MRM acquisition rates improve peak shapes by increasing the number of data points across the peak and providing better resolution of near-eluting lipid isomers</li></ul></li></ul>From sample collection to data processing - PFAS program at State Hygienic Lab<ul><li>The State Hygienic Lab at University of Iowa had the PFAS program running for couple of years and is accredited and certified by the state, EPA, and UCMR5 program to test for PFAS in water samples. However, testing encompasses different environmental and biological matrices. This presentation will look at the program and focus on:<ul><li>Approaches from sample collection to data processing and reporting.</li><li>Testing for PFAS in water matrices (drinking, raw, well, surface, and groundwater) based on EPA 537.1 and 533 FAS methods.</li><li>New method for PFAS in biological samples.</li></ul></li></ul>Q&amp;A roundtable: &nbsp;quantitation and screening<ul><li>Moderated by: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEX</li><li>Panelists include:<ul><li>Roberto Riccio, Biochemie Lab</li><li>Shane Karnik, Senior Laboratory Director, Aliri Bioanalysis</li><li>Ebru Selen, Biologics Applications Scientist, SCIEX</li><li>Paul Baker, Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX</li><li>Craig Butt, Senior Manager, Scientific Marketing, Applied Markets, SCIEX</li></ul></li></ul><blockquote>Moderator: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEXWith over ten years in product development, Ian is the Senior Technical Product Manager for nominal mass LC-MS/MS systems at SCIEX. He has also served as a Technical Product Manager and Global Technical Marketing Manager. Ian began his SCIEX career as an Application Scientist, focusing on biologics bioanalysis and DiscoveryQuant software validation.Before SCIEX, Ian was the Lead Analytical Scientist at Custom Biologics, overseeing LC-MS/MS method development and clinical trial analysis. He also served as a Senior Scientist at Affinium Pharmaceuticals, focusing on metabolic stability profiling and enzyme inhibition assays.Ian holds a postdoctoral position at McMaster University, specializing in antibiotic resistance and enzyme inhibitor screeningPresenter: Roberto Riccio (Biochemie Lab)Presenter: Shane Karnik (Senior Laboratory Director, Aliri Bioanalysis)Presenter: Ebru Selen (Biologics Applications Scientist, SCIEX)Presenter: Paul Baker (Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX)Presenter: Craig Butt (Senior Manager, Scientific Marketing, Applied Markets, SCIEX)</blockquote>

SCIEX summit Day three: Quantitation and screening

Food allergies are a public health and regulatory challenge, with an estimated 1–2% of adults affected by food allergies. Risk analysis and management of allergens within the food and beverage industry rely on accurate allergen analysis.High-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS) could be the future of food allergen analysis.In this webinar, our expert speaker will discuss how HPLC-HRMS can detect a wide range of allergens in a single run, reducing the time and effort involved in food analysis. He’ll also highlight how integrating HPLC with HRMS approaches enhances accuracy and makes allergen detection more reliable, ensuring robust food safety and compliance with regulatory standards.<h4>Attend this webinar to:</h4><ul><li>Learn how to multiplex allergen detection &nbsp;</li><li>Improve the accuracy and speed of your food analysis &nbsp;</li><li>Discover a complete workflow including sample preparation and data analysis</li></ul><blockquote>Presenter: Prof. Jens Brockmeyer (Head of Department, Food Chemistry, University of Stuttgart, Germany)</blockquote>

Technology Networks: Moving Towards the Next Generation of Food Allergen Analysis

Moving Towards the Next Generation of Food Allergen Analysis

How HPLC-HRMS can detect a wide range of allergens in a single run, reducing the time and effort involved in food analysis.

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #8: Instruments &amp; Software for Non-Target Screening | F&amp;E Summer School - Day 2 - Module 4</h3>This session will focus on presenting in detail the data evaluation of HRMS-Screening experiments. Firstly, we will explain the data-independent acquisition modes MSE and HDMSE. We will describe an intelligent and efficient peak detection algorithm of the HRMS data. The waters_connect software supports us with several tools for the challenging non-Target screening. We will present a selection of discovery tools which collect additional information about the unknown masses to reduce the number of potential candidates. This includes a binary comparison to find differences between samples or the get information on common fragments etc. In addition, multivariate statistics can also be used as a powerful tool for identifying significant non-Target masses. Finally, the determination of the molecular formula of potential candidates and a possible search in online libraries will be presented.<h5>What you'll learn:</h5><ul><li>Data acquisition in depth (MSE, CCS)</li><li>waters_connect Software &amp; Intelligent Peak Detection</li><li>UNIFI App and Workflow</li><li>Binary Compare - find the Differences</li><li>Discovery Tools – Common Fragments, Mass defect filtering, Halogen Match and more</li><li>Multivariate statistics</li><li>Pattern Targeting Application</li></ul><blockquote>Presenter:&nbsp;Christoph Thomas (Principal Sales Applications Chemist, Waters Corporation)Christoph Thomas is a Principal Sales Applications Chemist from the Central Europe Customer Success Team based in Eschborn, Germany. He has more than 25 years’ experience in the field of Chromatography and Mass Spectrometry and is familiar with several application areas using Quadrupole and Tof instruments. Within the last decade his projects were mainly focused on the waters_connect Screening Solution in food and environmental screening, toxicology screening, metabolite identification and in the field of extractables and leacheables.Presenter:&nbsp;Gunnar Weibchen (Principal MS Sales Dev Specialist, Waters Corporation)Gunnar studied Chemistry and obtained a PhD in Organic and Analytical Chemistry in Hamburg. After working as an Application Chemist in the demo laboratory at Varian, has been with Waters in Sales and Marketing since 2010</blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Software and Instruments for NTS

This session will focus on presenting in detail the data evaluation of HRMS-Screening experiments. 

Clinical blood analysis is crucial for monitoring health and diagnosing diseases, with blood-based biomarkers playing a key role.However, current detection methods often lack sufficient coverage. Mass spectrometry-based proteomics provides a robust solution for in-depth plasma proteome analysis, but precise sample preparation is essential.This webinar will guide you on maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum. Dr. König and Dr. Wendt will discuss developing an optimized, automated workflow for comprehensive plasma biomarker discovery, showcasing results from two complementary techniques: LC-MS and Olink.<h4>Attend this webinar to:</h4><ul><li>Discover how to efficiently prepare blood-derived samples, including plasma and serum</li><li>Explore how to implement a fully automatable, reproducible and standardized plasma workflow with scalable throughput</li><li>Achieve comprehensive plasma proteome characterization, addressing the dynamic range challenge for deeper insights</li><li>Learn to apply LC-MS-based proteomics combined with affinity-based methods to discover novel disease biomarkers</li></ul><blockquote>Presenter: Dr. Ann-Christine König (Proteomics Core Facility Manager, Helmholtz Zentrum Munich) Presenter:&nbsp;Dr. Fabian Wendt (Product and Application Manager, Mass Spectrometry, Tecan)</blockquote>

Technology Networks: Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum.

Unlocking the Plasma Proteome: Advanced Profiling

This session will provide a comprehensive introduction to the user interface, ensuring you can navigate with ease. Discover the various acquisition modes available and learn how to select the best one for your specific application.Additionally, we will explore the innovative Intelligent Reflex feature, highlighting its benefits in boosting your productivity.Whether you’re new to MassHunter or looking to refine your skills, this webinar is designed to enhance your understanding and efficiency.<blockquote>Presenter: Christian Hegmanns (Application Specialist LC/MS, Agilent Technologies, Waldbronn, Germany)Christian Hegmanns is a state-certified food chemist and works at Agilent as an LC/MS application specialist.With over 10 years of experience in the field of instrumental analysis, Christian develops customer-oriented solutions for a variety of applications with a focus on food analysis.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part I – Basic Overview

Join us for an insightful webinar where we delve into the essentials of MassHunter Acquisition Q-TOF. 

LCMS webinars focusing on LC/TOF - page 14