Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.<h4>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</h4>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.<div><div>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (Figure 1).Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493">While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.<h4>Key takeaways</h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</h4>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.<h4>Online SPE analysis provides key benefits like:</h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul>Let’s explore what this technique looks like in practice.<h4>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</h4>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"><h4>The process for analyzing a hops standard solution is straightforward:</h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"> on the SPE column</a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"> analytical column </a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (Figure 3).</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"><h4>Online SPE for analyzing α- and β-bitter acids in real hops samples</h4>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (Figure 4).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4>From carefully procured samples to tasty sips</h4>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.<ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf">check out our application note today</a>.<h4>FAQs</h4><div><div><h5>What are the key compounds in hops analyzed by HPLC?</h5>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</div><div><h5>What column types are recommended for hop acid separations?</h5>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</div><div><h5>What’s the best workflow for hops analysis?</h5>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</div></div><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#UHPLC #Automation</blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

Webinars LabRulezLCMS Week 27/2026

The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607">A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;<h4>Evaluation of measurement procedure performance&nbsp;</h4>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;<ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"><h4>Characterisation of candidate reference materials&nbsp;</h4>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;<ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4>Other ILCs&nbsp;</h4>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).

Interlaboratory comparisons other than proficiency testing

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In this part of the course we will deal with sample preparation: why, how and which sample preparation is the most suitable for the target analyte?SPE, LLSE, or syringe filter?&nbsp;<blockquote>Presenter: Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Sample Preparation in HPLC

Why, how and which sample preparation is the most suitable for the target analyte?

<h4>Key Learning Points:</h4><ul><li>Identify the common challenges associated with solid phase extraction methods &nbsp;</li><li>Understand and troubleshoot the recovery, contamination and reproducibility issues &nbsp;</li><li>Learn how to find and apply solutions to develop robust and reproducible extraction methods for their sample preparation needs</li></ul><h4>Who Should Attend:</h4><ul><li>Any scientists who wants to learn about solid phase extraction and it’s associated challenges, common troubleshooting steps and solutions to develop robust and reproducible extraction methods for their sample preparation needs</li></ul><blockquote>Presnter: Namrata Saxena (Technical Manager, Phenomenex)Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Sydney, Australia. As a member of the global technical team, she is responsible for assisting external customers as well as internal marketing and sales teams of Phenomenex for multifarious technical queries. She specifically support company’s bio-products portfolio through external and internal seminars/webinars, trainings and also contribute in biologics related technical content creation.Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH.Namrata has a post graduate degree with specialization in Analytical Chemistry. Her competence and focus lies in biotherapeutics characterization through liquid chromatography, antibodies and oligonucleotide chemistry, gene therapy and new bio-therapeutic modalities.</blockquote>

Phenomenex: Solid Phase Extraction Troubleshooting Tips and Tricks

Solid Phase Extraction Troubleshooting Tips and Tricks

Identify the common challenges associated with solid phase extraction methods.

Our guest speakers will discuss automation within their lab and how the Andrew+ liquid handling robot has helped streamline their sample preparation workflows for proteomic analysis.Automation is fast becoming a priority for labs who are looking to improve liquid handling pipetting procedures for simple, yet critical, protocols. The automation of liquid handling procedures allows labs to streamline workflows, helping to improve.<ul><li>Reproducibility - Laboratory automation eliminates most human errors resulting in more reliable data and improved productivity.</li><li>Productivity - Andrew+ combines the flexibility of single channel pipetting (0.2 μL to 10 mL) with the higher throughput of multichannel pipetting.</li><li>Health in the Lab - Pipetting requires repetitive movements (of up to 500 times per day for the average user) that can lead to Musculo-skeletal disorders like repetitive strain injury. The use of automated liquid handling reliefs users from this high throughput pipetting burden, helping to improve health within the lab setting.</li></ul><h4>Testimonials from our guest speakers</h4><h5>Dr Harry Whitwell, Imperial College London</h5>Andrew+ has been a huge asset to the group and is used continuously to support routine sample prep from protein assay to digestion, desalting, peptide assay, sample-standardisation, plate formatting and study reference generation, helping to substantially minimise batch-variation. Data from repeated dilution series across months shows excellent reproducibility in pipetting accuracy and stability with inter-assay CV &lt;5% and R2 above 0.99.<h5>Dr Pavel Shliaha, MRC</h5>In our lab, we use automation in attention-heavy workflows, e.g. concentration normalisation and SPE. The main workflows that we are using the Andrew+ is for concentration normalisation for proteomics. We also plan to use it for SPE for proteomics. My favourite things about the Andrew+ robot are as follows: user interface, great technical support, ability to easily switch pipettes, without the necessity for calibration.<h5>Dr Colleen Maxwell, University of Leicester</h5>We previously found it a major challenge to find sample preparation solutions to match the large scale required for our large cohort clinical proteomics studies without compromising on flexibility – we used to rely on laborious protocols requiring manual pipetting steps in small batches, we have found that these are no longer suitable, and are likely to introduce unwanted batch effects over the long term. Andrew+ has revolutionised our workflows, offering versatility beyond standard bottom-up proteomics digestion and clean-up. Andrew+ is such an adaptable system, so has had a transformative impact on all aspects of experimental design, and has been adopted throughout all our bottom-up proteomics workflows at the van Geest Multiomics Facility in the University of Leicester.<blockquote>Presenter:&nbsp;Dr Harry Whitwell (Lecturer and Proteomics Platform Lead, Imperial College London)Dr Harry Whitwell has been proteomic lead at the National Phenome Centre at Imperial College London since June 2020. Alongside this position, he was appointed Lecturer in Proteomics and Integrative Data Analysis in October 2023. Building on funding MRC, EPSRC and BBSRC, he has established a far-reaching facility, supporting projects across the UK. His research focuses on combining multiple analytical modalities to understand disease including mass spec-imaging, proteomics and network-based data analysis techniques.Presenter:&nbsp;Dr Pavel Shliaha (Head of Proteomics and Metabolomics, MRC, Laboratory of Medical Sciences)Dr Pavel Shliaha completed his Ph.D. in proteomics at the laboratory of Professor Kathryn Lilley in Cambridge, UK, focusing on organelle proteomics. He then pursued a postdoctoral project in Professor Ole Jensen's group at the University of Southern Denmark, concentrating on histone PTM profiling and intact protein analysis. Pavel briefly worked at Memorial Sloan Kettering Cancer Center as a postdoc before moving to MSD (New Jersey). Throughout his career, Pavel has been interested in automation and high-throughput sample processing. In particular, he has experience working with Hamilton Star, Opentrons, and Andrew+ systems.Presenter: Dr Colleen Maxwell (Postdoctoral Researcher, University of Leicester)Colleen is a post-doctoral research associate at the Leicester van Geest Multiomics Facility at the University of Leicester. She completed her undergraduate studies in Chemistry at Imperial College London, graduating in 2019. During this time, she spent a year working in a pharmaceutical company, where she gained her initial exposure to analytical chemistry and mass spectrometry. Motivated by her interest in this field, Colleen pursued a PhD at the University of Leicester, focusing on the use of shotgun and targeted proteomics to develop and validate multiplexed biomarker panels for coronary artery disease. After completing her PhD in 2023, she continued her research as a postdoctoral associate, expanding her work to include new projects that integrate deep cardiac MRI phenotyping with proteomics in collaboration with the cardiac imaging team at Leicester. Colleen is passionate about the automation and standardisation of proteomics to ensure highly reproducible and high-throughput biomarker studies in large clinical cohorts</blockquote>

Waters Corporation: Streamlined Proteomics with the Power of Andrew+

Streamlined Proteomics with the Power of Andrew+

How the Andrew+ liquid handling robot has helped streamline their sample preparation workflows for proteomic analysis.

Measurement of FT4 and FT3 is typically performed by immunoassay, however these can be prone to suffer from interferences and a lack of selectivity. Currently, the accepted gold standard for the measurement of FT4 and FT3 is using equilibrium dialysis (ED) and combined with the use of LC-MS/MS can overcome the issues with immunoassay in the measurement of FT4 and FT3. However, the equilibrium dialysis procedure can be quite time consuming and often being performed overnight.Here, we describe a simple clinical research method to perform ED using a commercially available device followed by sample preparation by solid phase extraction (SPE) and UPLC-MS/MS analysis using the Waters™ ACQUITY™ UPLC and analytically sensitive Xevo™ TQ Absolute Mass Spectrometer for clinical research. Data analysis and review has been simplified with the use of the waters_connect™ with QUAN Review Software. This has allowed for the complete workflow including equilibrium dialysis to be performed within a typical working day.Simplified Equilibrium Dialysis for the UPLC-MS/MS Analysis of Free Testosterone (FT) for Clinical ResearchHistorically, the measurement of free testosterone in human serum has presented a challenge for laboratory scientists, with such a large portion bound and the complexity and time involved in “gold standard” equilibrium dialysis. With a simple, fast, workflow combined with the analytical sensitivity afforded by the Waters Xevo™ TQ Absolute Mass Spectrometer, a newly developed, precise, accurate and selective clinical research method will be presented.<blockquote>Presenter: Robert Wardle (Senior Clinical Scientist, Waters Corporation)Robert Wardle is currently a Senior Scientist employed at Waters Corporation in Wilmslow, UK, performing research and development of applications and products for Clinical Research. His primary area of research is vitamins and in particular vitamin D metabolites, steroid hormones and in-depth knowledge of offline and online automation.Robert joined Waters in 2010 as a Clinical Validation Scientist, playing a key role in verification activities for the MassTrak XE Immunosuppressants kit available in the EU. He moved into his current role as a Senior Scientist in 2013, where he developed clinical research methods for a range of applications including vitamin D metabolites, estrogens, a large panel of steroid hormones and more recently this application on free T4 and free T3.Presenter: Stephen Balloch (Senior Clinical Scientist, Waters Corporation)Stephen Balloch graduated from the University of York (UK) with a Master’s degree in Chemistry, which included a strong focus on analytical chemistry. Subsequently he worked as a laboratory scientist in a GLP compliant research facility, progressing to Study Director. And, since 2010, he has been part of the clinical team at Waters (Wilmslow, UK). His principal focus has been on the development and validation of bioanalytical methods according to CLSI guidelines, with a focus on therapeutic drug monitoring. Additionally, he has supported newborn screening, vitamin and steroid analysis, including this clinical research method for free testosterone</blockquote>

Free Testosterone and Free T3/T4

Simplified Equilibrium Dialysis and SPE Method for the UPLC™-MS/MS Analysis of Free Thyroxine (FT4) and Triiodothyronine (FT3) for Clinical Research.

Manual sample preparation has its limitations. As sample throughput increases, so does fatigue, leading to a decline in data quality. This issue is significant across various markets but is especially critical in drug development and the production of therapeutics in biotechnology.However, employing automation can enhance data consistency and improve reproducibility. Additionally, automation reduces the need for costly sample reruns and frees up users’ time for other tasks.Attend this webinar to:<ul><li>Discover the benefits of automation and identify who should consider adopting it</li><li>Explore comprehensive workflow solutions that guide you from sample preparation to data-driven decisions, tailored to biopharma and proteomics applications</li><li>Learn how the updated AssayMAP Citation Index assists users in finding published methods that utilize the AssayMAP Bravo for biopharma applications</li><li>See how compliant software can facilitate the transition of methods from non-compliant to compliant labs, such as moving from early to late stages of drug discovery</li></ul><blockquote>Presenter: Kenda LJ Evans, PhD (Automation Workflow Specialist, Agilent Technologies)Kenda received her Ph.D. from The University of Texas Health Science Center in San Antonio in the field of Pharmacology with a focus on the signal transduction pathway for the Serotonin 1A (5-HT1A) receptor. Kenda’s post-doctoral work occurred at the University of Houston.</blockquote>

Technology Networks: Automation Answers to Your Liquid Handling Challenges

Automation Answers to Your Liquid Handling Challenges

Discover the benefits of automation and identify who should consider adopting it

<div><div><div>Pure MHC, a pioneer in immunopeptidomics, has partnered with Bruker, a leader in state-of-the-art mass spectrometry, to demonstrate one of Pure MHC’s immunopeptidomics workflows. In this Live from the Lab webinar, tissue sample processing, data acquisition with a Bruker timsTOF Ultra, and data analysis will be reviewed as tools for the development and validation of HLA peptide targets for immunopeptidomic development.Key Learning Objectives:<ul><li>Get insights into Pure MHC's sample preparation strategies to maximize the yield of immunopeptides from tissue samples</li><li>Discover how the unparalleled sensitivity and robustness of the Bruker timsTOF Ultra empowers users to maximize the potential of their immunopeptide samples</li><li>Understand data processing workflows for both DDA and DIA data processing</li></ul><blockquote>Presenter: Curtis McMurtrey, Ph.D. (Director of Immunoproteomics, Pure MHC, LLC, Oklahoma City, OK, USA)Dr. McMurtrey earned his Ph.D. in Microbiology and Immunology from the University of Oklahoma Health Sciences Center in 2010. He currently serves as the Director of Immuno-Proteomics at Pure MHC, LLC.Dr. McMurtrey has studied HLA and its role in immunity since 2005. He is a member of American Society for Histocompatibility and Immunogenetics (ASHI) and holds the distinction of having won four ASHI Top Scholar Awards, more than any other scientist in the history of ASHI. Dr. McMurtrey is most notable for his work on West Nile virus epitope discovery and validation with HLA-A*02:01 and B*07:02; epitopes he discovered have been successfully tested in a WNV vaccine (collaboration with Drs. Hansen and Diamond at Washington University). His most recent accolades come from discovery of MR1 ligands from Mycobacterium tuberculosis (Mtb) as well as over 200 novel ligands from Toxoplasma gondii utilizing Deep Ligand Sequencing (DLS).Dr. McMurtrey has expertise in BSL-3 containment techniques, protein purification, HLA ligand isolation, HPLC separation, mass spectrometric analysis, ELISPOT, and multicolor flow cytometry as well as provides bioinformatics expertise. To date, he has directed mass spectrometry (both IDA and SWATH) acquisition as well as comparative analysis and interpretation for a wide variety of MHC ligands including those from tuberculosis, toxoplasmosis, influenza, WNV, HIV and multiple cancers. Dr. Curtis McMurtrey oversees DLS acquisition and analyses and also manages database library collection.Presenter: Jason R. Krawic, Ph.D. (Business Development Manager, Pure MHC, LLC, Oklahoma City, OK, USA)Dr. Krawic earned his Master’s degree in Biomolecular Sciences from Central Connecticut State University in 2012 before returning to academia and earning his Ph.D. in Microbiology and Immunology from the University of Oklahoma Health Sciences Center in 2024. He currently serves as the Director of Business Development at Pure MHC, LLC.Dr. Krawic began his scientific career teaching Microbiology, Biology, and Chemistry to community college students in Connecticut and Oklahoma. He has studied HLA and non-classical MHC molecules since 2018. He is most recognized for discovering multiple isomeric conformations of MR1 ligands, as well as his work in MHC Class I and Class II ligands and cancer immunotherapies.Dr. Krawic’s expertise ranges from classical microbiology and bacteriology to HPLC separation, protein purification, negative ion mode small molecule mass spectrometry, DDA, DIA and MRM acquisition and a variety of bioinformatics tools. In addition to his scientific career, Dr. Krawic has more than a decade of experience in sales and retail management. With this skill set he combines his science and sales backgrounds to serve Pure MHC’s customer base.Presenter: Kris Looney, MBA (President, Pure MHC, LLC, Austin, TX, USA)As President of Emergent Technologies, the manager of Pure MHC, Kris Looney is responsible for conceiving and executing key business initiatives, developing strategic plans, and managing business processes including accounting, marketing, intellectual property, and business development. Kris has more than two decades of experience as a business generalist with a background in start-ups, management, accounting, marketing, negotiation, and sales.Before joining Emergent, Kris served as the COO and Interim CEO of the American Red Cross of Central Texas, where he balanced multiple stakeholder needs with service responsibilities in a competitive philanthropic environment. Prior to that, he was the CFO of Transformation Enzyme Corporation, where his financial management and business development helped a bootstrapped entrepreneurial start-up become a leader in the nutritional supplement market. Kris has a B.A. in marketing and an M.B.A. from the University of Texas at Austin.Presenter: Matt Willetts, Ph.D. (Proteomics Applications Manager, Bruker Scientific LLC, Billerica, MA, USA)Matt received his Ph.D. in mass spectrometry with Prof. Malcolm Clench at Sheffield Hallam University in the UK. He then joined Waters as the Technical Marketing Specialist for LCMS and MALDI products, focusing on application development for proteomics-based workflows. Matt joined Bruker in 2010 and is responsible for proteomics applications and support in North America.</blockquote>&nbsp;</div></div></div>

Bruker: Pure MHC's immunopeptidomics workflow with the timsTOF Ultra

Pure MHC's immunopeptidomics workflow with the timsTOF Ultra

Tissue sample processing, data acquisition with a Bruker timsTOF Ultra, and data analysis for immunopeptidomic development.

In today’s fast-paced research environment, optimizing lab workflows is crucial to maintaining productivity and ensuring high-quality results. Sample preparation is a critical step in chromatography and mass spectrometry, yet it often involves time-consuming and error-prone manual processes.Join us for an exclusive webinar with Dr. Jens Lättig, Senior Scientist at Tecan and Dr. Cassandra Rusher, European Technical Support Specialist at Eprep, where they’ll reveal how the Resolvex® Prep revolutionizes sample preparation for chromatography and mass spectrometry.Key learning objectives:<ul><li>Slash prep time: Discover how you can reduce your setup to just 5–15 minutes, freeing you to focus on critical tasks while Resolvex® Prep works autonomously.</li><li>Enhance accuracy: Learn how analytical syringes and specialized tools deliver consistent, reliable results – minimizing human error.</li><li>Achieve consistency: Find out how you can achieve repeatable results across various sample types and workflows, streamlining your processes.</li><li>Unlock versatility: Explore Resolvex® Prep’s built-in wash station, RFID deck scan, and seamless sample transfer, all designed to meet your research needs.</li></ul>Who should attend?<ul><li>Laboratory scientists and managers from analytical laboratories in: pharma, biopharma, biotech, the food industry, the environmental sector, agriculture, drug screening, proteomics, and metabolomics.</li></ul>Certificate of attendanceAll webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Jens Lattig, PhD (Sales Development Manager Proteomics &amp; MS Sample Prep, Tecan)Dr. Jens Lättig is a Sales Development Manager Proteomics &amp; MS Sample Preparation at Tecan. He has a PhD in biochemistry, and has worked in drug research projects in Germany and France. At Tecan, he is responsible for the Resolvex™ instruments and consumables portfolio in Europe.Presenter: Cassandra Rusher, PhD (European Technical Support Specialist, Eprep)Dr. Cassandra Rusher is a technical support specialist for the Resolvex Prep. at Eprep. She has a PhD in chemistry and a background specializing in chromatography including sample preparation. Her previous roles have included technical support and product management in the analytical chromatography sector. Her focus now is on supporting the automation of sample preparation methods across a range of industries that are utilizing analytical chromatography techniques.Moderator: Carrie Haslam (Associate Editor, SelectScience)</blockquote>

SelectScience: Efficient sample prep for expert mass spectrometry and chromatography

Efficient sample prep for expert mass spectrometry and chromatography

Join us for a webinar where we will reveal how the Resolvex® Prep revolutionizes sample preparation for chromatography and mass spectrometry.

Efficient sample prep for MS and chromatography

Did you know pipetting errors can quickly amplify across a normal workflow? And as protocols become more complex, there is an increased risk for experimental errors. Experimental error can propagate from many different sources:&nbsp;<ul><li>Incorrect calculations during experimental planning</li><li>Unstable or incorrect sample or reagent preparation</li><li>Incorrect instrument use or pipetting technique</li></ul>Join our live webinar to learn strategies for reducing pipetting errors to help you produce consistent and reliable results.Webinar agenda:<ul><li>Pipetting error propagation</li><li>Good science is control</li><li>Quick checking a pipette</li><li>Pipette performance verification</li><li>Pipette management software</li></ul>

Mettler-Toledo: Strategies for Reducing Pipetting Errors 

Strategies for Reducing Pipetting Errors

Join our live webinar to learn strategies for reducing pipetting errors to help you produce consistent and reliable results.

Strategies for Reducing Pipetting Errors 

Learn how to extend your NGS capabilities with the Magnis NGS Prep System.We are thrilled to invite you to our upcoming webinar on how to Extend your NGS Capabilities with the Magnis NGS Prep System.Discover the latest NGS technologies ensuring that labs produce consistent, high-quality data, no matter the application or sample requirements.In today’s fast-paced scientific environment, maximizing lab efficiency and productivity is crucial. The Magnis NGS Prep offers a comprehensive solution designed to streamline NGS library preparation workflows, reduce manual errors, and accelerate research outcomes.Webinar Learning:<ul><li>Automated Processes: Magnis System automates routine tasks, freeing up valuable time for scientists to focus on critical experiments.</li><li>Library Quality: &nbsp;Magnis System produces robust and reproducible results enabling an increased focus on data analysis.</li><li>Versatility: Magnis System adapts to your samples requirements with SureSelect catalog and custom panel options supporting a range of NGS applications.</li></ul><blockquote>Presenter: Mary Buchanan (Director of Product Marketing - Genomics Division, Agilent Technologies)Mary is Director of product management at Agilent, covering the NGS automation, microarray and functional genomics portfolios.Presenter: Andrea Citterio (Molecular Biologist, IRCCS E.Medea)Andrea has a Masters degree in industrial biotechnology and postgraduate student in medical genetics.Presenter: Fabiana Mambretti (Molecular Biologist, IRCCS E.Medea)Fabiana has a Masters degree in industrial biotechnology and postgraduate student in medical genetics.Presenter: Elena Panzeri (Molecular Biologist, IRCCS E.Medea)Elena successfully gained a Master’s degree in medical biotechnology, a PhD in neuroscience and postgraduate student in medical genetics.</blockquote>

Agilent: Extend your NGS Capabilities with the Magnis NGS Prep System

Extend your NGS Capabilities with the Magnis NGS Prep System

Discover the latest NGS technologies ensuring that labs produce consistent, high-quality data, no matter the application or sample requirements.

The complete Solution for Food Analysis: Presented by Retsch, CEM, Shimadzu and EltraAccurate and rapid food analysis is becoming increasingly important as regulations become stricter and consumers become more demanding. This expert day on food analysis will give you a comprehensive overview of the optimal steps from sample preparation to analysis, familiarize you with modern methods and bring you up to date.Registration is free of charge and without obligation.<div><h3>Reproducible sample preparation for food analysis&nbsp;</h3>Food occurs in different consistencies and is often inhomogeneous. For meaningful analysis, food laboratories require representative samples, which makes homogenization necessary as part of sample preparation.Ideally, this step should involve as little work as possible. The prepared subsample must represent both the laboratory sample and the initial sample. This would not be the case with an inhomogeneous sample, as some components of the initial sample could be over- or under-represented.</div><h5>In this webinar you will learn more about:</h5><ul><li>Homogenization, standard deviation, correct analysis values</li><li>How do I find the optimal method for my sample</li><li>Cryogenic grinding (dry ice, liquid nitrogen)</li><li>Suitable laboratory mills for homogenizing foodstuffs</li><li>Live demos of selected mills</li><li>Application examples</li><li>Grain size determination using sieve analysis</li></ul><blockquote>Presenter: Dr. Tanja Butt, Dr. Lena Weigold and Dr. Gerhard Beckers</blockquote>This webinar is part of a series of four webinars (Retsch, CEM, Shimadzu, Eltra). Please register for the time slot of each company which fits best. Americas should select the second time slot, Asia the first. Each presentation by the different companies requires an own registration. Here you can select the time slot for Retsch. For the other companies you need to visit the landingpage.

Reproducible sample preparation for food analysis

For meaningful analysis, food laboratories require representative samples, which makes homogenization necessary as part of sample preparation.

LCMS webinars focusing on Sample prep - page 15

Extend your NGS Capabilities with the Magnis NGS Prep System

Reproducible sample preparation for food analysis

Strategies for Reducing Pipetting Errors

Automation Answers to Your Liquid Handling Challenges

Efficient sample prep for expert mass spectrometry and chromatography

Sample Preparation in HPLC

Pure MHC's immunopeptidomics workflow with the timsTOF Ultra

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Streamlined Proteomics with the Power of Andrew+

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