Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Metrohm

Merck

Mettler-Toledo

C&EN

Modern titration offers a simple, yet highly accurate method to ensure the quality and safety of many products we consume daily. While not a complicated technique, there are some subtleties that can ensure your titration results are accurate. Simple electrode care and optimization of basic titration parameters are crucial to helping you obtain results you can trust.
During this webinar, Sagar Irrinki, Quality Control Manager at Coastal Gulf &amp; International, Inc. will share how his laboratory navigates executing these tests on various types of titration applications. and how to overcome challenges with helpful titration tips. Eduardo Simoes, Product Specialist for Titration at Metrohm USA, will provide guidance for electrode selection and best practices for electrode care and maintenance. Don’t miss the live Q&amp;A at the end to get all your questions answered by Sagar and Eduardo. 
<h5>Key Learning Objectives:</h5>
<ul>
<li>Understand potentiometric electrode care and maintenance </li>
<li>Learn how to overcome titration challenges with useful tips</li>
<li>Receive simple guidelines to remove doubt from titration results </li>
<li>Learn how to select the appropriate electrode for your titration</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone who wants to learn more about electrode care and maintenance</li>
<li>People interested in improving the consistency of their titration results</li>
<li>Anyone who needs a refresher on titration best practices</li>
<li>Chemists who want simple tips to improve confidence in titration analysis</li>
</ul>
<blockquote>
Presenter: Eduardo Simoes (Titration Product Specialist, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Sagar Irrinki (Quality Control Manager, Coastal Gulf &amp; International, Inc.)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

Mastering Potentiometric Titration through Effective Electrode Selection and Method Optimization

Simple electrode care and how to overcome challenges with helpful titration tips.

Mastering Potentiometric Titration

Karl Fischer (KF) titration is renowned for its rapid and precise measurement of water content in a wide variety of samples.
Understanding the factors that can impact the accuracy of your Karl Fischer titration process is crucial for ensuring optimal results.
Join us for an in-depth webinar that will delve into the critical factors affecting the Karl Fischer titration process and provide a comprehensive overview from start to finish. Participants will gain insights into essential procedures such as titer determination and explore critical applications.
This session is designed to enhance your understanding and improve the precision of your titration techniques.
<h4>Program Overview</h4>
<ul>
<li>Exploring the Components of a Volumetric Karl Fischer Titration Apparatus: Gain a detailed understanding of each element in the apparatus to enhance your practical knowledge.
</li>
<li>Introduction to Karl Fischer Reagents &amp; Standards: Familiarize yourself with the variety of reagents &amp; standards used in Karl Fischer titration and their specific applications.
</li>
<li>The Importance of Titer Determination: Learn why accurate titer determination is crucial for reliable results and discover the best practices for conducting it effectively.
</li>
<li>Analyzing Challenging Samples: Delve into practical examples that demonstrate how to handle and accurately analyze complex samples.
</li>
<li>Innovations in Titration Technology: Learn about our advanced titration technology that utilizes SmartChemicals to seamlessly transfer data to the Karl Fischer titrator.
</li>
</ul>
<blockquote>
Presenter: Bettina Straub-Jubb (Global Product Manager, Karl Fischer Titration at the Life Science Business of Merck KGaA, Darmstadt, Germany)
</blockquote>
<blockquote>
Bettina is the Global Product Manager for Karl Fischer titration and general titration within the Analytical Essentials team at Merck KGaA, Darmstadt, Germany. She has over 20 years of experience in various facets of titration, including Karl Fischer titration, instrumental analysis reagents, and reference materials, contributing significant expertise to her position. Bettina is deeply familiar with all steps of the titration process and collaborates closely with the application laboratories and R&amp;D department to continually enhance titration methodologies. She holds a degree in Chemical Technology from the Darmstadt University of Applied Sciences, Germany, and completed an apprenticeship as a medical technical laboratory assistant at the Medical Technical Academy in Esslingen, Germany.
</blockquote>
<blockquote>
Presenter: Thomas Hitz (Application Chemist, METTLER TOLEDO)
</blockquote>
<blockquote>
Thomas currently serves as a technical advisor for product development at METTLER TOLEDO, Nänikon, Switzerland. With over 15 years of experience in application development, sales, and customer training, he provides expert support for complex automation solutions in both general and Karl Fischer titration. He holds a degree in biochemistry from the University of Zürich and completed his PhD in 2003 at the Swiss Federal Institute of Technology Zurich in the Department of Material Sciences.
</blockquote>

Mettler Toledo: Optimize Your Volumetric Karl Fischer Titration

Optimize Your Volumetric Karl Fischer Titration

Critical factors affecting the Karl Fischer titration process and a comprehensive overview from start to finish.

Automatic titration plays a crucial role in ensuring precise and consistent results in various industry and research sectors, directly impacting the quality and safety of products we use daily. Despite the automation of the titration process itself, many manual steps still require an analyst&#39;s full-time attention. By optimizing titration procedures and using advanced instruments and accessories, it is possible to automate auxiliary tasks such as pipetting, diluting, and adding reagents. This optimization also enables the system to make decisions, such as whether to proceed with an analysis or take specific actions based on the results.
In this webinar, Monica Laurel, Analytical Manager at Dow Chemical, will share success stories of titration methodology optimization carried out by her and her team across various Dow Chemical units during her 20-year career. Eduardo Simões, Product Specialist for Titration at Metrohm USA, will demonstrate how automated systems can systematically reduce human error, ensure compliance with laboratory safety regulations, and enhance the accuracy and reproducibility of results.
Don&#39;t miss the live Q&amp;A at the end, where Monica and Eduardo will answer all your questions.
Please plan to attend if you:
<ul>
<li>Want to learn about titration procedure optimization</li>
<li>Are interested in reducing exposure to chemicals during titration analysis</li>
<li>Would like to understand how advanced software functions can monitor results and help with decision making</li>
<li>Are interested in improving the consistency of your titration results</li>
</ul>
Key Learning Points
<ul>
<li>Understand different ways to optimize your current titration methodology</li>
<li>Learn how a sample robot can help save analyst time by automating manual steps</li>
<li>Learn advanced software functions for better programming and monitoring data</li>
</ul>
<blockquote>
Presenter: Monica Laurel (Analytical Manager, Dow Chemical)
</blockquote>
<blockquote>
Monica Laurel is a Research Scientist with DOW Chemical in Freeport, Texas. She received her BS in Chemistry from the University of Texas at Austin. Monica joined Union Carbide in St. Charles, LA in 1995 as a process chemist. In 2004 she moved back to Texas and joined the Analytical Sciences group at DOW Chemical and began work in the Inorganic Lab focusing on titration technology. She has 20 years experience in numerous titration and Karl Fischer methods and techniques where she has worked to improve and automate titrations for implementing in DOW’s quality labs.
</blockquote>
<blockquote>
Presenter: Eduardo Simões (Titration Product Specialist, Metrohm USA)
</blockquote>
<blockquote>
Eduardo Simoes, MSc is the Titration Product Specialist at Metrohm USA in Riverview, Florida. He received his bachelor and masters in analytical chemistry at the University of Campinas, Brazil. Over the last 21 years with Metrohm, he specialized in potentiometry and electroanalysis, gaining extensive field experience in applications and instrument installations, technical support &amp; training.
</blockquote>

Metrohm: Real-World Titration Optimization: Advanced Techniques for Improved Results

Real-World Titration Optimization: Advanced Techniques for Improved Results

We will share success stories of titration methodology optimization and demonstrate how automated systems can systematically reduce human error.

Titration: Advanced Techniques for Improved Results

Titration is generally considered a fairly simple analytical technique. But what do you do, if your titration results are not what they should be? Enter Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters!In his webinar on September 18, Robert will show you what typical errors can occur during a titration – and how to avoid or remedy them. Focusing on troubleshooting, Robert will cover everything from the electrode to the setup of your titrator to titration parameters and calculation of &nbsp; the result. The following questions give you an idea of the scope and practical relevance of this webinar:<ul><li>How important is calibration?</li><li>Do I really need to stir the titration solution – and why?</li><li>What do I do, if several equivalence points are detected – or none at all</li><li>How to clean the electrode properly?</li></ul>These questions and many more will be answered step by step so that everyone can follow and understand. During a Q&amp;A session at the end of the webinar, you can ask Robert any question you may have about titration.<blockquote>Presenter: Robert Tobler (Senior Application Specialist Titration, Metrohm International Headquarters)After his apprenticeship as a chemical laboratory technician and further training as a biomedical analyst, Robert Tobler studied chemistry at the Zurich University of Applied Science in Waedenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since then, Robert has become an expert in applied titration sharing his expertise through the global network of Metrohm subsidiaries.</blockquote>

Metrohm: Troubleshooting titration: essential know-how for quality results

Troubleshooting titration: essential know-how for quality results

What typical errors can occur during a titration – and how to avoid or remedy them.

Troubleshooting titration: essential know-how for quality

Key takeaways from this webinar include:<ul><li>Understanding surfactants and their significance</li><li>How to accurately measure cationic, anionic, and non-ionic surfactants using potentiometric titration</li><li>The distinction between aqueous surfactant titration and two-phase surfactant titration, and when to use each method</li><li>How two-phase surfactant titration serves as a viable alternative to the widely used Epton Titration</li><li>Choosing the right potentiometric sensor for specific titrations</li></ul><blockquote>Presenter: Mona Wagner (Application Specialist Titration, to learn how to analyze surfactants by automatic potentiometric titration)</blockquote>

Metrohm: Best practice for surfactant analysis by potentiometric titration

Best practice for surfactant analysis by potentiometric titration

How to accurately measure cationic, anionic, and non-ionic surfactants using potentiometric titration.

Best practice for surfactant analysis

Dive into the future of Karl Fischer titration in this engaging webinar, where we will unveil the cutting-edge advancements and emerging trends shaping this critical analytical field.This session is specifically designed to highlight the innovative features of the EVA KF Titrators, which represent the forefront of titration technology. We will explore the integration of advanced technologies such as the Fast-Forecasting Amperometric (FFA™) Control Algorithm, which enhances the speed and reliability of water content determination, drastically reducing titration times without compromising result quality. Learn about the advanced drift management techniques that ensure consistent and reliable measurements, critical for industries where accuracy is paramount.Participants will also gain insight into how these technologies are being applied across various sectors, driving improvements in everything from pharmaceuticals to food and chemical manufacturing. The session will cover how the flexibility of the EVA KF titrators enables laboratories to adapt quickly to new regulations and testing requirements, supporting sustained compliance and operational excellence.<h4>Program Overview</h4><ul><li>Technological Innovations: Uncover the cutting-edge advancements in Karl Fischer titration, including the integration of the Fast-Forecasting Amperometric (FFA™) Control Algorithm.</li><li>Precision and Efficiency: Learn about new drift management techniques that enhance the accuracy and reliability of measurements, crucial for industries where precision is paramount.</li><li>Adaptability to Regulations: Explore how EVA KF Titrators' flexibility aids laboratories in swiftly adapting to new regulations and maintaining operational excellence.</li></ul><blockquote>Presenter: Erik GublerA Global Product Manager at METTER TOLEDO in Switzerland, specializing in analytical instruments. He holds a master's degree in chemistry from the Swiss Federal Institute of Technology (ETH Zurich). He has been an integral part of developing the next generation of automated titrators and software. His chemistry expertise and product insight ensure the continuous advancement and reliability of METTLER TOLEDO’s titrators across various industries.Presenter:&nbsp;Lukas FritscheAn Application Specialist at METTLER TOLEDO in Switzerland, specializing in titration technology. With a Ph.D. in chemistry from ETH Zurich, Lukas plays a pivotal role at METTLER TOLEDO in advancing application development and enhancing instrument capabilities. His work supports the continuous innovation and reliability of the company’s analytical instruments.</blockquote>

Mettler Toledo: The Future of Karl Fischer Titration

The Future of Karl Fischer Titration

The innovative features of the EVA KF Titrators, which represent the forefront of titration technology.

<h4>Eight Webinars and a Roundtable with Experts from Merck</h4>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.From understanding quality grades in inorganics and solvents to exploring sustainable practices in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to optimize your Karl Fischer titration, achieve precision in PFAS testing, and gain troubleshooting tips for LC-MS to effectively address common challenges and improve your laboratory results.The sessions will also address critical aspects of safety and sustainability, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.<h3>Day 3: Wednesday, 12 February</h3><h4>Optimize Your Karl Fischer Titration: Expert Insights for Enhanced Results</h4>With Karl Fischer titration, you can quickly and accurately determine water content in a wide variety of samples. The accuracy of the results depends on a well-defined titration application process. Pre-titration and titer determination are essential for obtaining correct results. The main challenges arise from samples that do not dissolve or that undergo side reactions in standard solvents. Learn the measures you can take to release water from such samples and the preferred methods to use—volumetry or coulometry. Additionally, discover how digital data transfer enables efficient, fast, and secure data integration with your titrator software.By attending this webinar, you will learn:<ul><li>The key steps in the Karl Fischer titration process</li><li>The importance of accurate pre-titration and regular titer determination</li><li>Effective methods for completely releasing water from your samples</li><li>How digital data transfer can streamline and secure your daily data transfer and documentation</li><li>Practical tips for overcoming daily challenges in Karl Fischer Titration</li></ul>Who should attend: This webinar will be especially valuable for individuals involved in developing Karl Fischer titration applications, performing titrations in QC labs or during in-process control, and those responsible for assessing and enhancing processes. This includes lab technicians, lab managers, and quality assurance managers across various segments.<blockquote>Presenter: Bettina Straub-Jubb, (Global Product Manager Analytical Technologies, Merck)</blockquote><h4>Beyond Safety Standards – Best Practices for Safe Handling of Hazardous Chemicals</h4>This webinar illustrates the role of safety in the lab as well as the challenges that can occur while handling hazardous goods in the daily lab work and how to overcome these challenges. Rules and regulations for daily handling of hazardous goods are discussed, and options are shown on how to improve daily processes regarding personal safety. The presentation focuses on the prevention of accidents, emergency help in case of chemical spillages, and safe and reliable cleaning of lab equipment. Join the webinar for an informative overview of safety solutions and tools for maximum safety and reliability in your lab.By attending this webinar, you will learn:<ul><li>Basic safety risks and rules for daily handling of hazardous goods and options for how to improve daily processes regarding personal safety</li><li>Packaging-related safety topics</li><li>Practical guidelines for handling hazardous chemicals in case of spillages</li><li>Lab equipment cleaning know-how for reliable analytical results</li></ul>Who should attend: Staff and head of chemical labs (e.g. quality control, research), as well as safety managers/safety engineers for chemical laboratories.<blockquote>Presenter: Annett Schneider, (Global Product Manager and Lead Expert for Safety Products and Essentials, Merck)&nbsp;</blockquote>

Merck: Webinar Series & Expert Roundtable

Webinar Series & Expert Roundtable (Day 3)

Part 1: Optimize Your Karl Fischer Titration and Part 2: Beyond Safety Standards – Best Practices for Safe Handling of Hazardous Chemicals

<h4>Eight Webinars and a Roundtable with Experts from Merck</h4>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.From understanding quality grades in inorganics and solvents to exploring sustainable practices in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to optimize your Karl Fischer titration, achieve precision in PFAS testing, and gain troubleshooting tips for LC-MS to effectively address common challenges and improve your laboratory results.The sessions will also address critical aspects of safety and sustainability, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.<h3>Day 5: Friday, 14 February</h3><h4>Roundtable Expert Discussion</h4>Our experts will come together to discuss the best practices to streamline your analytical workflows and enhance your efficiency. We will cover some questions asked during the week, as well as answer new questions during the roundtable discussion. &nbsp;<blockquote>Presenter: Ernie Hillier (Independent Pharmaceutical Consultant, EJH Consulting)</blockquote>

Webinar Series & Expert Roundtable (Day 5)

Webinar Series & Expert Roundtable (Day 5) - Roundtable Expert Discussion

<h5>By attending this discussion, you will:</h5><ul><li>Discover how automated control algorithms enhance precision and reduce titration times without compromising accuracy.</li><li>Understand how advanced drift management tools ensure reliable results, even in demanding conditions.</li><li>Explore how modern titration systems address safety and sustainability goals with features such as remote operation and support for greener solvents.</li></ul><h5>Event Overview</h5>For nearly a century, Karl Fischer titration has been the trusted method for determining water content in products ranging from pharmaceuticals to processed snacks. However, even reliable techniques face challenges. For example, antibiotics can be difficult to analyze due to their limited solubility in conventional solvents, often requiring additional heating steps to ensure accurate measurements. Environmental factors, such as humidity, can further complicate workflows by introducing drift and reducing precision.Join us for an expert discussion exploring how the latest innovations in Karl Fischer titration technology tackle these issues head-on. Panelists Nicolas Monney (Application Chemist, Mettler-Toledo) and Matteo Stefanoni (Senior Scientist, Mettler-Toledo) will answer questions related to advancements such as high-resolution sensors, low-dead-volume burets, and modular designs that improve accuracy, safety, and workflow efficiency. Learn how the EVA KF Titrator’s Fast Feedback Algorithm redefines titration workflows by adapting in real time to reaction conditions.<h5>Who Should Attend:</h5><ul><li>Scientists, lab managers, and analysts working in water determination across industries, including pharmaceuticals, food, biopharma, and materials science.</li><li>Professionals seeking practical solutions to improve accuracy, efficiency, and reproducibility in Karl Fischer titration workflows.</li><li>Anyone facing challenges with complex samples, drift, or evolving regulatory requirements in moisture analysis.</li></ul><h5>Share Your Challenges Across Industries</h5>Water determination plays a pivotal role in advancing quality and innovation. Do you struggle with reactive compounds, complex sample matrices, or meeting regulatory demands? This event offers the chance to have your specific questions addressed by experts.Submit your questions through the webinar registration form, and our panel will address selected challenges during the broadcast.<blockquote>Presenter: Nicolas Monney (Application chemist, Mettler-Toledo)Nicolas Monney holds a Ph.D. in Physical Chemistry. His research focused on studying electron transfer through proteins using low-temperature spectroscopy and ab-initio simulations.Nicolas began his career at Mettler-Toledo nearly ten years ago, taking on the role of Applications Specialist for Titration. In this role, he develops innovative titration applications and collaborates closely with the R&amp;D department to facilitate the development of new products. Additionally, he supports the sales force of Mettler-Toledo and its partners on titration projects worldwidePresenter: Matteo Stefanoni (Senior Scientist, Mettler-Toledo International)Matteo is a scientist with a strong academic background and experience in the fields of chemistry and material science. He earned his Master’s degree in Chemistry from the University of Milano and a PhD from ETH Zürich, focusing on electrochemistry. After completing his doctoral studies, Matteo spent two years as a postdoctoral researcher, further enhancing his expertise in the field. Currently, Matteo serves as a Senior Scientist at Mettler-Toledo, where he has spent the last five years developing advanced control algorithms for automatic titration instruments. Notably, he has dedicated four of those years to developing an innovative model-based control algorithm for Karl Fischer titration analysis, significantly improving the efficiency of this analytical technique.</blockquote>

Separation Science: Rethinking Karl Fischer Titration: How Smarter Algorithms Tackle Complex Water Determinations

Rethinking Karl Fischer Titration: How Smarter Algorithms Tackle Complex Water Determinations

Join us for an expert discussion exploring how the latest innovations in Karl Fischer titration technology tackle issues.

KF Titration: Smarter Algorithms Tackle Water Determinations

Analytical laboratories face continuous pressure to handle increasing sample volumes while delivering rapid, high-quality results. Quality managers and analysts must balance competing demands: maintaining precision and accuracy, meeting regulatory requirements, reducing environmental impact, and optimizing costs. These challenges are particularly acute in moisture analysis, where traditional methods often require significant time and resources, while inconsistencies between operators can impact reliability.Join industry experts as they explore modern approaches to moisture analysis in chemical, polymer, and petrochemical environments. This educational session will examine how laboratories can overcome common analytical challenges through the integration of complementary techniques. Through real-world examples and industry case studies, we'll discuss how combining reference methods with spectroscopic techniques can enhance laboratory efficiency. The session will conclude with insights from Lubrizol's experience in modernizing their analytical workflows, offering practical takeaways for laboratories looking to improve their testing procedures.<h5>Key Learning Objectives:</h5><ul><li>Fundamentals of Karl Fischer and NIR analysis</li><li>Strategies for workflow optimization and automation</li><li>Practical considerations for implementing new analytical methods</li><li>How OMNIS reduces waste while increasing speed and accuracy</li></ul><h5>Who Should Attend:</h5><ul><li>Analytical lab managers</li><li>Quality control or assurance mangers/directors</li><li>Process engineers or production managers</li><li>Anyone interested in learning about more efficient ways to do moisture analysis</li></ul><blockquote>Presenter: Bridget Brassell (Quality Assurance Improvement Manager, Lubrizol)Presenter:&nbsp;Eduardo Simoes, MSc (Titration Product Specialist, Metrohm USA)Presenter:&nbsp;Lenzi Williams, PhD (Spectroscopy Product Specialist, Metrohm USA)Presenter:&nbsp;Alexandra Taylor (Contributing Editor, C&amp;EN Media Group)</blockquote>

C&EN: From Challenges to Solutions: Revolutionizing Moisture Analysis with NIR and Titration

From Challenges to Solutions: Revolutionizing Moisture Analysis with NIR and Titration

Join industry experts as they explore modern approaches to moisture analysis in chemical, polymer, and petrochemical environments. 

LCMS webinars focusing on Titration - page 2