Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Metrohm

Mettler-Toledo

BioLogic

SelectScience

Insights and Strategies for Maintaining High Water Quality
Event Program
<ul>
<li>Common misconceptions about impurities in pharmaceutical waters</li>
<li>Differences between chemical and microbial properties in WFI and PW</li>
<li>Recommended approaches for measuring different impurities</li>
<li>Insights on maintaining a healthy water loop</li>
</ul>
Enhancing Quality and Safety in Pharmaceutical Manufacturing
Without proper impurity control, pharmaceutical manufacturers risk compromising the safety and efficacy of their products. Microbial and organic impurities can enter water systems at various points in the manufacturing process and impact quality and safety.
Misunderstandings surrounding controlling impurities in pharma waters can lead to improper measurement and treatment approaches.
It is a common belief that chemical and microbiological properties in Water for Injection (WFI) and Purified Water (PW) are the same in pharmaceutical water systems. However, this is far from the truth. These properties react differently and are distributed unevenly in storage and distribution loops, which can have different effects on the health of the water system.
In this live roundtable discussion, we will dispel the most common myths about microbes and organics and controlling impurities in pharma waters. We will discuss the differences between chemical and microbiological properties in WFI and PW and recommend measurement approaches for each impurity. Additionally, we will cover how to maintain a healthy water loop to ensure high quality and safety in pharmaceutical manufacturing.
<blockquote>
Presenter: Maura Rury (Senior Product Manager, METTLER TOLEDO Thornton)
</blockquote>
<blockquote>
Maura manages METTLER TOLEDO&#39;s Total Organic Carbon (TOC) product line, along with related services and training. Her prior roles include marketing, product management, and technical applications at LGC and Thermo Fisher Scientific. She received her Ph.D. in Analytical Chemistry from the University of Massachusetts, Amherst.
</blockquote>
<blockquote>
Presenter: Anthony C. Bevilacqua (Principal Scientist, METTLER TOLEDO Thornton)
</blockquote>
<blockquote>
Anthony Bevilacqua earned a doctorate in Analytical/Physical Chemistry from Tufts University. Since 1994, he has led several areas of Research and Product Development that include: impact of CO2 on pure water, ultrapure water as a conductivity solution standard, development of digital sensors, and expansion of real-time analytical parameters (conductivity, TOC, microbial detection, ozone, sodium, and silica) for measurement/control of high purity water systems, including pharmaceutical water systems. Anthony was Chair of the inaugural USP Pharmaceutical Water Expert Committee from 2000-2005, and re-elected from 2005-2010. Anthony’s role continued in the 2010-2015 and 2015-2020 cycles for the USP Chemical Analysis Expert Committee, and he is currently serving on the current committee for 2020-2025.
</blockquote>
<blockquote>
Presenter: Areen Kalantari (Global Segment Manager, Pharma &amp; Life Sciences, METTLER TOLEDO Thornton)
</blockquote>
<blockquote>
Areen Kalantari holds a B.S. in Biological/Chemical Engineering and a Master of Business Administration (MBA) in International Business. He has experience in both biotechnology applications and pharmaceutical waters. Areen is a voting member of the American Society of Mechanical Engineers – Bioprocess Equipment (ASME-BPE) standards committee. He contributes to the Process Instrumentation subgroup to help implement standards for the bioprocessing and pharmaceutical industries, focusing on conductivity, pH, TOC, and other process technologies.
</blockquote>

Mettler-Toledo: Controlling Impurities in Pharma Waters

Controlling Impurities in Pharma Waters

Dispeling the most common myths about microbes and organics and controlling impurities in pharma waters.

CVS (Cyclic Voltammetric Stripping) is the preferred method for monitoring the concentration of suppressors, brighteners, and levelers used in the production of high-quality printed circuit boards (PCBs) and in advanced packaging of semiconductors. Automating this monitoring can help manufacturers to significantly save time and effort while helping them to keep pace with increasing numbers of samples.
In her webinar, Barbara Zumbrägel, Product Manager Voltammetry at Metrohm International Headquarters, will present the latest technology to take fully automated quality control in PCB manufacturing with CVS to the next level of performance, efficiency, and convenience. Following the webinar, Barbara will be available for a Q&amp;A Session to answer any questions you may have.
Key learnings of this webinar:
<ul>
<li>How automated sample transfer and dosing can make your determination of suppressors, brighteners, and levelers more efficient</li>
<li>How to handle the increasing number of auxiliary solutions with an automated pipetting system</li>
<li>How to optimize your workflow by customizing methods in the viva software to meet the specific requirements of your analysis protocol</li>
</ul>
Who should attend?
<ul>
<li>Lab technicians and lab managers from the PCB, PWB, and semiconductor manufacturing industries</li>
</ul>
<blockquote>
Presenter: Barbara Zumbrägel (Product Manager Voltammetry, Metrohm International Headquarters)
</blockquote>
<blockquote>
Barbara Zumbrägel earned her diploma in chemistry from Technische Universität Clausthal, Germany, and joined Metrohm International Headquarters in 1999 as a product and application specialist for voltammetry. Since 2003, she has also played a significant role in the ongoing development of the Metrohm portfolio of CVS products and applications. In 2006, Barbara was promoted to Product Manager Voltammetry.
</blockquote>

Metrohm: Next level of CVS automation - how to increase the efficiency of your routine analysis

Next level of CVS automation - how to increase the efficiency of your routine analysis

Fully automated quality control in PCB manufacturing with CVS for the next level performance, efficiency, and convenience.

CVS automation - how to increase the efficiency

Event Program
<ul>
<li>Discover the fundamentals of multiparameter analysis and its role in increasing the productivity.</li>
<li>Explore real-world applications in pharmaceuticals, food &amp; beverage, and water testing.</li>
<li>Learn how automation in electrochemistry revolutionizes efficiency and empowers sustainable practices.</li>
<li>Gain insights into navigating compliance regulations seamlessly with METTLER TOLEDO&#39;s products and services.</li>
</ul>
In today&#39;s world, maximizing productivity is no longer optional. Across industries, there&#39;s a growing need for efficient and reliable methods to evaluate and optimize solutions. This is where multiparameter analysis emerges as a promising tool.
Precise pH measurement plays a crucial role in various sustainability initiatives. From ensuring optimal growth conditions in biofuel production to maintaining safe drinking water quality, pH data is essential. METTLER TOLEDO pH meters offer exceptional accuracy and reliability, making them an indispensable tool for reliable data in your sustainability journey.
The true strength of these meters also lies in their ability to seamlessly connect with other instruments for measuring critical parameters like refractive index, density, spectroscopic data etc. This enables analysis of a wider range of parameters simultaneously or in parallel. This comprehensive approach provides a holistic view of the samples, empowering one to optimize resource utilization, ensure product quality and safety, and develop sustainable solutions. By combining pH with other key parameters as mentioned above, deeper insights can also be obtained to minimize waste, maximize efficiency, and guarantee consistent product quality that adheres to safety regulations – all crucial aspects of sustainable practices.
This webinar, led by Dr. Reena Mascarenhas, Assistant Manager of Market Development, will explore multiparameter analysis and its role in streamlining the processes across various industries.
Join us for a Live Webinar on Multiparameter Analysis for Sustainable Solutions!
Register now and secure your spot!
For multiparameter analysis, what instruments can a pH meter be connected to?
Beyond parameters like conductivity, DO, ISE, and Redox, which can be measured by the same meter, a pH meter can be connected to other instruments like a refractometer, density meter, UV/VIS spectrometer, titrator, and balance.
In which industries can quality control labs leverage multiparameter analysis to improve their processes?
Multiparameter analysis empowers quality control labs across various industries. In the pharmaceutical sector, labs can ensure consistent drug quality by simultaneously measuring parameters like pH, conductivity, and concentration. The food and beverage industry, including the wine industry, benefits by monitoring key parameters like pH, conductivity, density, and dissolved oxygen to guarantee consistent taste, texture, and shelf life. It also plays a crucial role in monitoring safe drinking water (concentration of ions, pH, conductivity) and optimizing agricultural soil conditions (pH, conductivity, nutrient levels). By measuring multiple parameters simultaneously, labs achieve efficiency and ensure high-quality products across diverse industries.
<blockquote>
Presenter: Dr Reena Mascarenhas
</blockquote>
<blockquote>
Reena is an electrochemist, working for METTLER TOLEDO, with over five years of experience in training others in various aspects of the field. Her in-depth understanding, technical expertise, and engaging and informative training style make her a prominent speaker for this webinar.
</blockquote>

Mettler-Toledo: Multiparameter Analysis for the Lab: from Complexity to Simplicity

Multiparameter Analysis for the Lab: from Complexity to Simplicity

A Live Webinar for Optimizing Workflows for pH, Refractometry, and Density Determination.

Multiparameter Analysis for the Laboratories

Electrochemical methods are useful for determining various material properties of the components in electrochemical devices. Specifically, electrochemical impedance spectroscopy (EIS) performed on cells containing only the essential components can provide detailed information about each part of the device. This approach allows for a clearer distinction of each component&#39;s contribution to the overall performance. It also helps explain the electrochemical behavior of the complete system.
In this webinar, Dr. Christoffer Karlsson will show some examples of this methodology, focusing on battery component properties such as the MacMullin number of separators, tortuosity of active material coatings, and electrolyte conductivity. A workflow for simple and reliable impedance data fitting and parameter evaluation will also be shown.
Who should attend?
<ul>
<li>Professionals working on the design and optimization of electrochemical devices such as batteries, fuel cells, and supercapacitors, etc. </li>
<li>Professionals in industries related to energy storage, renewable energy, and electronic devices</li>
<li>Researchers interested in high-throughput EIS analysis</li>
<li>Researchers curious for better understanding of material transfer and interface tortuosity</li>
</ul>
Key Learning Points
<ul>
<li>Learn about device components of lithium-ion batteries</li>
<li>Learn how to determine conductivity, MacMullin number, tortuosity electrolytes by EIS</li>
<li>Learn how to set up a test cell for component analysis</li>
</ul>
<blockquote>
Presenter: Dr. Christoffer Karlsson (Senior R&amp;D Scientist, rhd instruments)
</blockquote>
<blockquote>
Dr. Christoffer Karlsson is a Senior R&amp;D Scientist at rhd instruments with a background in electrochemical analysis and material development for batteries and supercapacitors. With a proven track record of designing and carrying out investigative research projects, he has developed active materials and electrolytes for all-organic proton batteries, as well as active material formulations for lithium-metal batteries. He is keen to be a part of creating a sustainable society by developing new solutions for energy applications.
</blockquote>
<blockquote>
Presenter: Kia Williams, PhD (Electrochemistry Product Manager, Metrohm USA)
</blockquote>
<blockquote>
Kia has a PhD in chemistry from the University of South Florida. Her work included analyzing and characterizing Metal-Organic Frameworks and carbon materials for energy storage and conversion applications. She joined Metrohm in 2018 as the Electrochemistry Product Manager where she focuses on electrochemical and spectroelectrochemical applications.
</blockquote>

Metrohm: Accessing Battery Material Properties with Electrochemical Techniques

Accessing Battery Material Properties with Electrochemical Techniques

Focusing on battery component properties such as the MacMullin number of separators, tortuosity of active material coatings, and electrolyte conductivity.

Battery Material Properties with Electrochemical Techniques

Understanding and measuring pH accurately is crucial in both chemical and biological contexts, affecting a range of applications.
For example, in lithium batteries, precise pH control optimizes its performance and ensures safety. In paint, it determines the color consistency, stability, and product longevity. Similarly, in plant-based proteins, pH influences protein extraction and quality, affecting texture and nutritional value. However, the complex matrices and diverse physical properties of these samples often interfere with pH measurements, posing significant challenges.
Join our webinar to learn how to overcome these challenges and optimize your pH measurement results in both chemical and biological samples. Let our experts guide you through advanced techniques, best practices, and practical solutions tailored to different applications.
<h4>Program Overview</h4>
<ul>
<li>Introduction to pH measurement: Learn about the importance of pH accuracy in fields like chemistry and biology.
</li>
<li>pH in chemicals: Discover how precise pH monitoring ensures chemical stability, reactivity, and shelf life. Learn tips for managing pH-dependent chemical processes for optimal results.
</li>
<li>pH in biological samples: Explore the critical role of pH in physiological functions, enzyme activity, and disease diagnosis.
</li>
<li>Techniques and best practices: Discuss pH measurement techniques, calibration, buffers, and overcoming common challenges.
</li>
<li>Interactive Q&amp;A session
</li>
</ul>
<blockquote>
Presenter: Dr Reena Mascarenhas
</blockquote>
<blockquote>
Dr. Reena is a seasoned electrochemist with over 5 years of dedicated experience at METTLER TOLEDO. Her in-depth knowledge and expertise in the field have made her a sought-after speaker for live webinars. With a strong academic foundation and a passion for electrochemical advancements, Dr. Reena is committed to sharing her insights and contributing to the growth of the scientific community.
</blockquote>

Mettler Toledo: How to Measure pH in Chemicals and Biological Samples

How to Measure pH in Chemicals and Biological Samples

How to overcome these challenges and optimize your pH measurement results in both chemical and biological samples.

Whether you are developing new materials for batteries electrodes, testing full or half cells or working on new electrolytes, electrochemical methods are at the core of your daily activities.&nbsp;In this webinar we will cover theoretical and practical aspects of various electrochemical techniques for battery research and testing. From the most fundamental CC cycling, to more advanced &nbsp;techniques such as galvanostatic intermittent titration technique (GITT), this webinar is meant to help you navigate this field and chose with confidence with techniques are most fit for your research.This webinar is accessible to anyone working in the field of battery, materials research, with or without a background in electrochemistry.<blockquote>Presenter: Thomas Touzalin, PhD.&nbsp;Product Manager at Metrohm Autolab, helping the electrochemistry researchers achieve their goals by providing the right solution for any research problems as well as offering technical support for the Autolab product range.</blockquote>

Metrohm: Electrochemical techniques for battery research and testing

Electrochemical techniques for battery research and testing

Theoretical and practical aspects of various electrochemical techniques for battery research and testing.

In laboratory analysis, pH measurement is a fundamental yet often overlooked hero. However, even the most straightforward tasks can lead to unexpected pitfalls if not performed with precision.Imagine a lab where every pH reading is spot-on—analyses are consistent, reliable, and yield top-notch results. This isn’t just a dream; it can be your reality! Join our informative webinar, where we'll guide you through the essentials of pH theory and measurement processes. We’ll also provide expert tips on sensor maintenance and troubleshooting, ensuring you have all the guidance to measure pH confidently.With a solid foundation in pH measurement practices, you’ll sharpen your skills and transform the overall quality of your results. Don’t miss this opportunity to enhance your expertise in pH measurement—let’s embark on this journey together!Event Program<ul><li>The Fundamentals of pH: A Deep Dive into the Basics</li><li>pH Measurement Setup and Workflow: Simplifying and Streamlining Your Measurement Process</li><li>Sensor Maintenance and Troubleshooting: Optimizing Your Sensor Performance</li><li>Good Electrochemistry Practices: Elevating Your Expertise for Precise Results</li><li>Interactive Q&amp;A Session with Our Speaker</li></ul><blockquote>Presenter: Dr. Reena MascarenhasDr. Mascarenhas is a seasoned electrochemist with over 5 years of dedicated experience at METTLER TOLEDO. Her in-depth knowledge and expertise in the field have made her a sought-after speaker for live webinars. With a strong academic foundation and a passion for electrochemical advancements, Dr. Mascarenhas is committed to sharing her insights and contributing to the growth of the scientific community.</blockquote>

What Is pH?

Engage, Explore, and Learn the Key Concepts of pH Measurement.

Fuel cells and electrolyzers are paving the way for a sustainable energy future. But unlocking their full potential demands a deep understanding of the electrochemical processes at work.Electrochemical methods are essential for understanding the complex phenomena underlying the operation of electrolyzers and fuel cells, advancing clean energy innovation.This webinar offers three focused sessions:<ul><li>Scientific Insights: Francisco Jose Fernandez Carretero will explore key research questions addressed through potentiostats and other electrochemical techniques.</li><li>Best Practices: Olivier Roux will discuss Electrochemical Impedance Spectroscopy (EIS), emphasizing how to avoid experimental artifacts and ensure accurate measurements.</li><li>Advanced Tools: Cedric Georges will present BioLogic’s cutting-edge instruments, optimized for advanced electrochemical research.</li></ul>The session will conclude with a Q&amp;A, providing attendees an opportunity to engage directly with the speakers.Hosted by Dr. Lindsey Monger and Dr.GianLuca Santoni, this webinar will provide actionable strategies for both academic and industrial researchers.

BioLogic: Electrochemical methods for the characterization of electrolyzers and fuel cells

Electrochemical methods for the characterization of electrolyzers and fuel cells

This webinar will provide critical insights into electrochemical methods and best practices, helping you refine your research and achieve more reliable results.

Electrochemical methods for electrolyzers and fuel cells

Join experts from METTLER TOLEDO as they highlights sources of potential errors associated with pH measurements that could significantly impact the accuracy, repeatability and reliability of your results. With the focus on both qualitative and quantitative errors and how to prevent them, you will gain practical insights to elevate your pH measurement and enhance your workflow progression.Plus, if you have a particular question you'd like to ask, Jonasz Puchała, Product and Application Specialist at METTLER TOLEDO, will offer top tips and expert advice during the live Q&amp;A session.<h5>Key learning objectives:</h5><ul><li>Explore the overall model to understand different influencing factors</li><li>Gain insights into quantifiable and unquantifiable errors</li><li>Explore theoretical information about the cause of the errors</li><li>Learn how to improve measurement accuracy for respective errors</li><li>Learn how to prioritize improvements</li></ul><h5>Who should attend?</h5><ul><li>Those working within R&amp;D, QC and manufacturing within biotechnology, pharmaceutical, and food and beverage industries.</li></ul><h5>Certificate of attendance</h5>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Jonasz Puchała (Product and Application Specialist pH Lab , METTLER TOLEDO)Jonasz Puchała studied Medical Biotechnology at the University of Opole, Poland, with a specific focus on clinical biochemistry and biotech engineering leading to the completion of his Master's in 2022. Since graduating, he has been working in the laboratory equipment industry as a Product and Application Specialist at METTLER TOLEDO.Moderator: &nbsp;Olivia Long (Editorial Team, SelectScience)</blockquote>

SelectScience: How to reduce and prevent pH measurement errors

How to reduce and prevent pH measurement errors

Join experts from METTLER TOLEDO as they highlights sources of potential errors associated with pH measurements

Whether you are developing novel electrochemical sensors, optimizing electrode materials, or enhancing detection strategies, electrochemical techniques are essential to your research.In this webinar, we will cover both theoretical and practical aspects of electrochemical methods for sensor development. From fundamental techniques like cyclic voltammetry and square wave voltammetry to advanced methods such as electrochemical impedance spectroscopy and absorptive stripping voltammetry, this session will help you choose the right approach for your application with confidence.This webinar is planned to last about 45 minutes and is accessible to anyone working in sensor research with or without a background in electrochemistry.<div><blockquote>Presenter: James Taylor (PhD. Application Scientist, Metrohm Autolab)</blockquote></div>

Metrohm: Electrochemical techniques for sensors research

Electrochemical techniques for sensors research

This webinar explores both the theoretical and practical aspects of electrochemical methods for sensor development.

LCMS webinars focusing on Electrochemistry - page 2