This workflow confirms and quantifies 31 drugs of abuse in oral fluid (including THC) in a 4.5‑minute LC run on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar mass spectrometer</a> using full scan + targeted MS2 (tMS2) with both HCD and CID fragmentation. With 1 µL injection and 0.1% formic acid in water/methanol mobile phases, the method supports ~13 samples/hour (up to ~320/day continuous operation) while maintaining confirmation performance via ion ratios.<blockquote><h5>Method at a glance</h5><ul><li>What matrix is tested? Oral fluid</li><li>How many drugs? 31 drugs of abuse (panel includes THC)</li><li>Run time? 4.5 minutes</li><li>Throughput? ~13 samples/hour → up to ~320 samples/day (24‑hour operation)</li><li>Injection volume? 1 µL</li><li>Mobile phases? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B)</li><li>MS acquisition? Full scan + targeted MS2 (tMS2)</li><li>Fragmentation? Compound‑optimized HCD and CID in the same targeted list</li><li>Confirmation strategy? Ion ratios with ±20% tolerance (typical confirmatory approach)</li></ul></blockquote><h3>Why oral fluid—and why speed matters</h3>Oral fluid is attractive for clinical and forensic toxicology because collection is simple and the matrix aligns well with high‑volume workflows (workplace/occupational, roadside, and other settings). The challenge is building a method that’s simultaneously:<ul><li>Broad enough for today’s drug panels</li><li>Sensitive enough to reach required cutoffs</li><li>Fast enough to keep sample queues under control</li><li>Confirmation‑ready (consistent product ions and ion ratios)</li></ul>A 4.5‑minute method is a practical lever here: compress runtime, raise throughput, and still keep confirmation quality by optimizing MS/MS conditions per compound.<h3>The LC method</h3>A short, efficient separation is the foundation of the workflow:<ul><li>UHPLC system: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Thermo Scientific Vanquish Horizon UHPLC</a></li><li>Column: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424">Thermo Scientific Accucore Biphenyl</a>, 50 × 2.1 mm, 2.6 µm</li><li>Mobile phase A: 0.1% formic acid in water</li><li>Mobile phase B: 0.1% formic acid in methanol</li><li>Injection volume: 1 µL</li><li>Gradient runtime: 4.5 minutes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 1: Thermo Scientific Vanquish Horizon UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg 120w," sizes="100vw" width="747" height="969"><h3>The MS method (Full scan + targeted MS2 with HCD + CID)</h3><h5>Acquisition approach</h5>This method uses a “two‑layer” strategy for quantitation + confirmation:<ol><li>Full scan (broad coverage, context, quantitative support)</li><li>Targeted MS2 (tMS2) (confirmation‑focused product ions, ion ratios)</li></ol><h5>The key differentiator: HCD and CID in one targeted list</h5>Instead of forcing every analyte into a single fragmentation style, the method uses both:<ul><li>HCD (beam‑type fragmentation)</li><li>CID (resonance‑type fragmentation)</li></ul><h5>Why that improves method development:</h5>Drugs of abuse span multiple chemistries. A single collision approach often works “okay” for most compounds—but one or two analytes can become persistent problems (weak qualifiers, unstable ion ratios, excess noise). Having both HCD and CID available in one method lets you optimize per compound and move faster.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 2: Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg 245w," sizes="100vw" width="645" height="377"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 3: Full scan &amp; targeted MS2 methods, HCD and collision energies used." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg 245w," sizes="100vw" width="768" height="279"><h3>Why CID + HCD flexibility improves confirmation</h3>In this workflow, HCD performed well for most compounds. But for buprenorphine, HCD produced one dominant product ion with additional low m/z noise and noticeable residual precursor. Using CID generated two high‑intensity product ions and delivered consistent ion ratios across the calibration range.When your confirmation hinges on stable ion ratios, the ability to switch fragmentation mode (not just collision energy) is a method‑development advantage—especially for “problem analytes.”<h3>Controllable ionization</h3>Oral fluid methods often deal with limited sample volume and matrix effects. This workflow pairs low injection volume with ionization control using the Thermo Scientific OptaMax Plus HESI source.<ul><li>The OptaMax Plus HESI source supports high probe temperature capability (up to 650 °C).</li><li>In this study, a vaporizer temperature of 500 °C was used to help achieve sensitivity and improve relative peak areas across drugs of abuse.</li></ul>Why this matters: If you’re injecting only 1 µL, ionization stability and efficiency can be the difference between “meets cutoff” and “misses at LOQ.”<h3>Sample preparation</h3>Oral fluid panels become more challenging when THC is included because most drugs of abuse are basic, while THC is largely neutral, and that can stress extraction chemistry.In this workflow, oral fluid calibrators and samples were extracted using a mixed‑mode approach designed to recover THC alongside other drugs:<ul><li>Calibrators prepared across eight levels (broad dynamic range)</li><li>Extraction performed using mixed‑mode chemistry tips (SCX/WAX) in a dispersive SPE‑style workflow</li><li>Steps automated on a liquid handling platform to support high‑throughput consistency</li><li>Samples dried and reconstituted prior to LC‑MS analysis</li><li>Only 1 µL injected per run</li></ul>Even if your lab uses different automation or consumables, the method structure (fast LC + full scan + targeted MS2 with dual fragmentation) transfers well.<blockquote><h5>Throughput math</h5><ul><li>4.5 minutes per injection → 60 / 4.5 = 13.3 injections/hour</li><li>That’s ~13 samples/hour in typical phrasing</li><li>Over 24 hours: 13.3 × 24 = ~320 samples/day (continuous operation)</li></ul>If you run a single shift, just scale accordingly (e.g., ~106/day for an 8‑hour shift, excluding QC and overhead).</blockquote><h3>Performance summary</h3><h5>Sensitivity and linearity</h5><ul><li>Linearity from LOQs as low as 0.25 ng/mL up to 1,000 ng/mL (panel-dependent)</li><li>For THC specifically: LOQ at 1 ng/mL, with a dynamic range extending to 1,000 ng/mL in this evaluation</li><li>Across the panel, LOQs were reported as lower than recommended confirmation cutoffs referenced in SAMHSA guidance and NSC Tier 1 cutoffs (study-specific outcome)</li></ul><h5>Confirmation via ion ratios</h5><ul><li>Ion ratios evaluated using ±20% tolerance (typical confirmatory acceptance framework)</li><li>The workflow produced consistent ion ratios across calibrator levels for the full panel in this evaluation</li></ul><h5>Case sample comparison (real-world relevance)</h5>When compared against a validated triple quadrupole method for case study samples, the Stellar MS workflow:<ul><li>Confirmed the positive hits observed by the reference method</li><li>Reported a few additional positives for select analytes, attributed to lower LOQs</li><li>Highlighted an operational efficiency point: 1 µL injection in this method versus 20 µL injection in the compared method</li></ul><h3>FAQ</h3><div><div><ol><li>How long does the LC‑MS run take for the 31‑drug oral fluid panel? 4.5 minutes per injection using a short UHPLC gradient. &nbsp;</li><li>How many drugs of abuse can Stellar MS detect in this workflow? 31 drugs of abuse, including THC, using full scan + targeted MS2. &nbsp;</li><li>What MS/MS approach is used? A combined strategy: full scan plus targeted MS2 (tMS2) for confirmation. &nbsp;</li><li>Why use both HCD and CID? Because some analytes fragment better—and produce more stable confirmation ions/ion ratios—under one mode versus the other. Using both increases flexibility and speeds method optimization. &nbsp;</li><li>What injection volume is used? 1 µL of extracted sample. &nbsp;</li><li>What mobile phases are used? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B). &nbsp;</li><li>How many samples can you run per day? At 4.5 minutes/injection, throughput is ~13 samples/hour or up to ~320/day with continuous operation (actual throughput depends on QC and scheduling).</li></ol></div></div><h3>What makes this workflow “easy” to develop on Stellar MS</h3>Here’s the short list of features that directly reduce method‑development friction:<ul><li>HCD or CID in one method → optimize per compound, not compromise</li><li>Fast MS2 acquisition speeds (up to 140 Hz for MS2) → more data points across narrow peaks</li><li>Fast polarity switching (true 5 ms) → supports mixed panels efficiently</li><li>Adaptive RT (real‑time retention time window adjustment) → helps reduce reinjections when retention times drift (e.g., column aging) and supports method transfer from longer to shorter gradients</li><li>OptaMax Plus HESI source control → supports sensitivity at low injection volumes</li></ul>If you’re building a fast oral fluid confirmation workflow—or updating an existing DOA panel—this 4.5‑minute method shows how Stellar MS can combine speed, confirmation-ready MS2, and fragmentation flexibility (HCD + CID) in a single targeted workflow. Reach out to learn how to adapt the targeted list, collision settings, and scheduling strategy to your lab’s cutoffs and throughput goals.<blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#MassSpectrometry #OralFluid #DrugsOfAbuse #MethodDevelopment #HighThroughput</blockquote>

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

<h2><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h2>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/644">35th International Symposium on Chromatography</a></h4>📆6. - 10. September 2026📍Prague, Czech Republic<h3>REGISTRATION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register HERE for the ISC 2026</a>Late Registration until 25 August 2026</blockquote><h3>INDUSTRY ACTIVITIES</h3><h4>Activity 1: Discussion Session: Industry &amp; Education</h4><ul><li>Monday, 7 September 2026 | 16:30-17:50</li><li>Place: Club A</li><li>Moderators: Tony Edge and Deirdre Cabooter&nbsp;</li></ul><h5>Concept:</h5>This discussion session will explore the relationship between education in separation science in academia and the expectations of industry, focusing on how universities and companies can better collaborate to prepare graduates for successful careers in analytical laboratories and R&amp;D environments. The goal of the session is to provide perspectives from both academia and industry on how education in chromatography and related separation techniques can evolve to better reflect current technological, methodological, and regulatory challenges.<h5>Discussion Topics:</h5><ul><li>Bridging academia and industry: how both sectors perceive current cooperation and where improvements are possible&nbsp;</li><li>Graduate preparedness: which skills and competencies are often missing when entering industrial roles, and in which areas graduates typically excel&nbsp;</li><li>Approaches to teaching separation science: how chromatography and related analytical techniques are taught across different universities and regions&nbsp;</li><li>Industry support for education: examples of collaboration such as internships, industrial placements, joint teaching activities, or access to instrumentation&nbsp;</li><li>Experiences with academia–industry partnerships: what models of cooperation have proven effective in practice&nbsp;</li><li>Future perspectives: how education in separation science should adapt to emerging trends such as automation, digitalization, sustainability, and regulatory requirements&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Shimadzu&nbsp;</li><li>Novartis&nbsp;</li><li>Jelle De Vos, RIC Group&nbsp;</li><li>Davy Guillarme, University of Geneva&nbsp;</li><li>Caterina Temporini, University of Pavia&nbsp;</li></ul><h4>Activity 2: Career Presentation&nbsp;</h4><ul><li>Monday, 7 September 2026 | 18:00-19:00</li><li>Place: Club A</li><li>Moderators: Adrian Clarke and Martina Catani</li></ul><h5>Concept:</h5>This session will provide an overview of career opportunities in separation science across industry, highlighting the wide range of professional pathways available to graduates and experienced scientists working in chromatography and related analytical disciplines.Participating companies will introduce their organizations, present typical roles involving separation techniques, and share insights into the skills and competencies they seek in candidates. The session is intended to support students, early-career researchers, and experienced professionals interested in exploring career options in industry sectors.<h5>Discussion Topics:&nbsp;</h5><ul><li>Overview of career paths involving chromatography and separation science&nbsp;</li><li>Entry-level opportunities for graduates and PhD holders&nbsp;</li><li>Positions for experienced scientists and technical specialists&nbsp;</li><li>Roles in R&amp;D, analytical development, quality control, regulatory science, application support, and instrument development&nbsp;</li><li>Skills and competencies valued by employers in separation science&nbsp;</li><li>Internships, graduate programs, and early-career development opportunities&nbsp;</li><li>Mobility between academia and industry&nbsp;</li></ul><h5>Format:</h5>Short presentation providing:<ul><li>overview of different carrier paths&nbsp;</li><li>introduction to the company and its activities related to separation science&nbsp;</li><li>examples of typical roles and career progression paths&nbsp;</li><li>information about current or expected hiring needs&nbsp;</li><li>advice for candidates interested in pursuing careers in industry&nbsp;</li><li>the session should include time for informal discussion, allowing participants to interact with company representatives and learn more about specific opportunities.&nbsp;</li></ul><h4>Activity 3: Job Fair</h4><ul><li>Monday, 7 September and Tuesday, 8 September 2026 | 12:30-14:00</li><li>Place: Club A</li><li>Main organizer: Adrian Clarke</li></ul><h5>Concept:</h5>The ISC 2026 Job Fair provides a structured opportunity for direct interaction between companies active in separation science and conference participants seeking career opportunities. The session is designed to facilitate efficient, focused conversations between employers and candidates interested in positions related to chromatography and analytical science.Participating candidates will submit their CV in advance allowing companies to review profiles prior to the event and identify potential matches based on expertise, experience, and career interests.<h5>Format:</h5>The Job Fair will follow a structured rotation format:<ul><li>Each participating company will be assigned a dedicated table&nbsp;</li><li>One company representative will meet candidates individually&nbsp;</li><li>Each candidate will have 5 minutes per meeting to introduce their background and interests&nbsp;</li><li>Participants will rotate between companies at timed intervals&nbsp;</li><li>Session timing will be managed by the organizer, with a signal indicating when to move to the next meeting&nbsp;</li></ul><h5>Candidates:</h5>Participants interested in joining the Job Fair are asked to:<ul><li>submit their CV before August 23rd &nbsp;</li><li>indicate areas of expertise and career interests&nbsp;</li><li>specify whether they are seeking internships, entry-level roles, or experienced positions&nbsp;</li><li>specify if you cannot be available on a certain day e.g. owing to poster presentation&nbsp;</li><li>send these materials to: <a href="mailto:careers@isc2026.org">careers@isc2026.org</a>&nbsp;</li></ul><h5>Companies:</h5>Confirmed participating companies to date include:<ul><li>Novartis&nbsp;</li><li>RIC Group&nbsp;</li><li>Shimadzu&nbsp;</li><li>further companies to be added&nbsp;</li></ul>Potential employers please register your interest to <a href="mailto:careers@isc2026.org">careers@isc2026.org</a> to participate in the speed-dating (first come first served basis).“Jobs position” board will be available in the Careers fair in the foyer in the symposium venue and attendees can visit the company booths in the exhibition hall or discuss with recruiting companies during the conference.<blockquote>Recruiters please send an email with your open positions to <a href="careers@isc2026.org">careers@isc2026.org</a> by the 23rd August and bring the job adverts to post on the jobs board (there will also be the possibility to upload the open positions to the Symposium app).</blockquote><h4>Activity 4: Discussion Session: Artificial Intelligence in Separation Science: From Concept to Practice</h4><ul><li>Tuesday, 8 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Bob Pirok</li></ul><h5>Concept:</h5>Artificial intelligence (AI) and data-driven approaches are increasingly influencing analytical chemistry, offering new possibilities for method development, data interpretation, process optimization, and decision support. This discussion session will explore how AI is currently being applied in separation science, with a particular focus on practical implementation in industry environments.The session aims to provide a realistic overview of the current state of AI adoption, highlighting both successful applications and remaining challenges. Contributions from industry and academia will help clarify where AI already delivers value, where expectations may exceed current capabilities, and what developments are needed to enable broader implementation.<h5>Discussion Topics:&nbsp;</h5><ul><li>Current applications of AI and machine learning in separation science&nbsp;</li><li>Use of AI in chromatographic method development and optimization&nbsp;</li><li>Data analysis, peak detection, and automated interpretation of complex datasets&nbsp;</li><li>AI-supported troubleshooting and predictive maintenance of analytical instruments&nbsp;</li><li>Integration of AI into regulated environments (e.g., pharmaceutical industry)&nbsp;</li><li>Data requirements, data quality, and standardization challenges&nbsp;</li><li>Comparison between academic developments and real-world industrial implementation&nbsp;</li><li>Barriers to adoption, including regulatory considerations, validation requirements, and transparency of models&nbsp;</li><li>Future outlook: digital laboratories, autonomous method development, and AI-assisted decision-making in analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h4>Activity 5: Discussion Session: Sustainability in Separation Science</h4><ul><li>Wednesday, 9 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Gertrud Morlock</li></ul><h5>Concept:</h5>Sustainability has become an increasingly important priority in analytical science, driving the development of greener methods, reduced solvent consumption, improved energy efficiency, and more responsible laboratory practices. While academic research frequently proposes innovative sustainable approaches, their implementation in industry is often influenced by additional considerations such as robustness, regulatory compliance, cost efficiency, scalability, and long-term reliability.This discussion session aims to explore how sustainability principles are applied in real industrial environments and how collaboration between academia and industry can help translate promising research concepts into practical, validated solutions.By bringing together perspectives from both sectors, the session will highlight opportunities to better align research priorities with industrial needs and identify realistic pathways toward more sustainable separation workflows.<h5>Discussion Topics:&nbsp;</h5><ul><li>Reduction of solvent consumption and the transition to more environmentally friendly mobile phases&nbsp;</li><li>Miniaturized and more efficient separation and extraction techniques&nbsp;</li><li>Energy consumption and efficiency of laboratory processes and analytical instrumentation&nbsp;</li><li>Lifecycle considerations for columns, consumables, and laboratory waste&nbsp;</li><li>Metrics and approaches for measuring and evaluating the “greenness” of analytical methods&nbsp;</li><li>Regulatory constraints and requirements that may limit the implementation of greener analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory contributions from invited speakers representing industry, academia, and technology providers, followed by interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Sébastien Rolle, Shimadzu&nbsp;</li><li>Ken Cook, Thermo Fisher Scientific&nbsp;</li><li>Andreas Otto, Agilent&nbsp;</li><li>Janusz Pawliszyn, University of Waterloo&nbsp;</li><li>Giorgia Purcaro, University of Liège&nbsp;</li></ul>

Industry program at ISC 2026

We are delighted to invite food scientists from academia and industry, representatives of (inter)national agencies, control authorities, governmental and commercial laboratories to attend <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/928">RAFA 2026</a> (12th Symposium on Recent Advances in Food Analysis), that takes place 3–6 November 2026 in Prague..<blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/">SUBMIT ABSTRACT</a> FOR POSTER PRESENTATION by August 31, 2026Do not miss the opportunity to present your latest results at RAFA 2026.</blockquote><h5>Next deadlines:</h5><ul><li>August 31, 2026: 2nd window – &nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/sponsors_exhibitors_media_partners_info.html">Application for participation as a Sponsor or Exhibitor</a></li><li>September 30, 2026:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/registration.html">Registration at the regular rate</a></li><li>Abstract submission – Last minute posters (May not appear in the RAFA 2026 program)</li></ul></li></ul>Since its first edition in 2003, RAFA has grown into a prestigious international event, typically welcoming up to 850 participants from more than 60 countries worldwide, with the support of more than 50 sponsors, exhibitors, media and supporting partners.RAFA 2026 will provide an overview of contemporary trends in the ANALYTICAL &amp; BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discuss the CHALLENGES AND NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.View the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/invited_speakers.html">list of distinguished speakers</a> who have accepted our invitation to RAFA 2026 and will present current hot topics and future perspectives in food analysis!<blockquote>👉More details on the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/scientific_programme.html">RAFA 2026 preliminary program</a> can be found HERE.👉OUR <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/about_sponsors_exhibitors_partners.html">SPONSORS, EXHIBITORS, MEDIA and SUPPORTING PARTNERS</a></blockquote>You are kindly invited to join and contribute to our exciting scientific program and unforgettable social events!We look forward to meeting you again in Prague!<ul><li>Prof. Jana Pulkrabova &amp; Dr. Stefan van Leeuwen, Symposium Chairs</li><li>Prof. Michele Suman, Symposium Co-chair</li><li>Prof. Jana Hajslova &amp; Prof. Michel Nielen, Symposium Honorary Chairs</li></ul>

RAFA 2026 - Deadline for Abstract Submission for poster presentation is coming

Article

Product

Events

Application

Food

Video

RAFA provides an overview of contemporary trends in ANALYTICAL & BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discusses CHALLENGES / NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.

Recent Advances in Food Analysis (RAFA)

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Tosoh Bioscience

Agilent Technologies

Phenomenex

Shimadzu Corporation

KNAUER

Waters Corporation

When it comes to LC analysis, a common question for users is, “Which LC column should I choose?” The column is the heart of the LC system, which plays a pivotal role in ensuring high-resolution separation. The fundamental knowledge of column selection can hence help you decide the optimal column for your specific application.
In this second series of Understanding LC Columns webinar, we will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC. We will discuss some of the common applications for each of these separation techniques. The difference in column chemistry and column specifications can significantly impact your separation. This webinar will also cover the different column chemistry and the considerations when selecting a column for the different applications.
Learning Objectives
<ul>
<li>Understand the different mode of separation and their application in liquid chromatography.</li>
<li>Understand the different column chemistry and their separation mechanisms.</li>
</ul>
<blockquote>
Presenter: Dr. Derrick Tan (Application Chemist (Consumables), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Dr. Derrick Tan obtained his PhD in chemistry from Nanyang Technological University, Singapore. He has more than 7 years of research experience in small molecules, nucleic acids, peptides and protein synthesis, separation and purification. He joined Shimadzu (Asia Pacific) Pte Ltd in 2021 as an Application Chemist (Consumables). At Shimadzu, he is responsible for the technical support and application of consumables in chromatography and spectroscopy. Products include LC and GC columns, UV/Vis cuvettes, sample vials and accessories.
</blockquote>

Shimadzu: Understanding LC Columns - Difference in Column Types and Phases

Understanding LC Columns - Difference in Column Types and Phases

We will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC.

Understanding LC Columns - Column Types and Phases

At the analytical scale, liquid chromatography (LC) is widely used by scientists to separate, quantify, and characterize components in mixtures. At the preparative scale, LC is primarily used to purify samples for further downstream analysis. The adoption of preparative LC into a workflow, with larger columns and instrumentation, can appear to be a daunting task. However, by following some fundamental guidelines and a little practical advice, it can be quite simple.
<h5>Key learning objectives</h5>
<ul>
<li>Become familiar with the analytical to preparative chromatography workflow</li>
<li>Learn strategies to efficiently develop methods for purification success</li>
<li>Know how to avoid or recover from common mistakes</li>
<li>Understand how to think like a purification scientist</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Analytical and medicinal chemists who want to learn more about purification or preparative LC, or regarding scale-up from analytical to preparative LC</li>
<li>Lab managers supporting purification labs</li>
<li>Scientists who are curious about incorporating purification into their laboratory workflow</li>
</ul>
 
<blockquote>
Presenter: Scott Bollen (Business Development Manager EMEA, Agilent Technologies)
</blockquote>
<blockquote>
Scott specializes in small molecule LC columns, ranging from HPLC to UHPLC. Scott has worked at Agilent for 17 years. He started as a technical support manager, and now supports new LC products in R&amp;D as well as the entirety of the existing small molecule LC column portfolio in business development.
</blockquote>

Agilent Technologies: Scale Up from Analytical to Prep with Agilent Prep LC Columns

Scale Up from Analytical to Prep with Agilent Prep LC Columns

Become familiar with the analytical to preparative chromatography workflow and learn strategies to efficiently develop methods for purification success.

From Analytical to Prep with Agilent Prep LC Columns

CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES
This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins. Understanding the fundamentals of bioseparations will enable you to develop methods more efficiently. At the end of this series, you will be up to date on cutting edge principles and innovations surrounding bioseparations.
Who should attend? 
<ul>
<li>Those interested in a refresher on foundational principles and tips for bioseparations </li>
<li>Those revisiting which columns and mobile phases are optimal for which workflows </li>
<li>Those looking for an overview of industry advancements and new offerings</li>
</ul>
<blockquote>
Presenter: Bill Warren (Principal Product Manager – Chemistry Technology Center, Waters Corporation)
</blockquote>
<blockquote>
Bill received his Masters in Microbiology and Immunology from University of Louisville School of Medicine. In 1986, he joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Following four years as a field based technical sales representative for Waters, Bill enjoyed a tenure as Manager, Core Product Evaluation Laboratory within the Waters Worldwide Marketing Organization. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.
</blockquote>
<blockquote>
HOST: Zerene Manaloto (Chemistry Products Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Zerene is a Chemistry Product Marketing Manager within the Americas Field Marketing team. She holds a Chemical Engineering Bachelor’s degree from Stevens Institute of Technology. Early in her career, Zerene was a research scientist at Bristol-Myers Squibb in Process Research &amp; Development involved in preparative scale purification and glass plant operations to support IND and clinical supplies of drug candidates. Prior to joining Waters, Zerene was in consumable field sales, supporting key accounts and customers within the pharmaceutical and clinical industries.
</blockquote>

Waters Corporation: CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS

This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins.

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS 

PRINCIPLES OF SIZE EXCLUSION CHROMATOGRAPHY: OPTIMIZING PARTICLE SIZE, PORE SIZE, AND COLUMN DIMENSIONS

PRINCIPLES OF SIZE EXCLUSION CHROMATOGRAPHY: COLUMNS

<ul>
<li>Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs
</li>
<li>Listen in to a column selection discussion, which includes pore volume and column dimensions to ensure monomer and aggregate resolution, and optimize mobile phases for complex characterizations
</li>
<li>Learn about SEC combined with nHRMS, and how to select a SEC column
</li>
</ul>
Who Should Attend:
Any scientists wanting to learn how to combine their size exclusion chromatography and High-resolution Mass Spec for improvements in their mAb characertizations. 
<blockquote>
Presenter: Lorne Nelson (Senior Scientist - Field Applications, Phenomenex)
</blockquote>

Phenomenex: SEC-nHRMS for the Characterization of mAbs

SEC-nHRMS for the Characterization of mAbs

Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs

Key learning objectives
<ul>
<li>Guidance to making Adjustments to chromatographic methods
</li>
<li>Column Phases, Particle Sizes, Internal diameter, Flow rate
</li>
<li>Composition, Injection volume, Dwell volume
</li>
<li>System suitability
</li>
</ul>
<blockquote>
Presenter: Dr.Heiko Behr (Senior Business Development Manager, Europe)
</blockquote>

Phenomenex: Harmonisation of Ph.Eur. 2.2.46 and USP 621

Harmonisation of Ph.Eur. 2.2.46 and USP 621

Guidance to making Adjustments to chromatographic methods...

Key learning objectives
<ul>
<li>Reversed Phase-Ion Pair LC for Oligos - Challenges and Optimization
</li>
<li>Rapid Isolation of Oligo Therapeutics from Biological Samples
</li>
<li>High-throughput, RPC Purification
</li>
</ul>
<blockquote>
Presenter: Mahesh Thondawada (Business Development Manager Biopharma/Biologic)
</blockquote>

Analytical BioSolutions for Therapeutic Oligonucleotides

Reversed Phase-Ion Pair LC for Oligos - Challenges and Optimization...

The objective of this webinar is to guide LC users on LC column care &amp; troubleshooting.
Audience will learn about ways to maintain LC column performance and prolong column lifetime. Guidance to troubleshoot issues caused by LC column will be share in this webinar.
Learning Objectives:
<ul>
<li>Learning on maintaining LC column performance and prolong column lifetime
</li>
<li>Troubleshooting issues caused by LC column
</li>
</ul>
<blockquote>
Presenter: Yap Siew Qi (Ph.D.) (Consumables Technical Specialist, Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
Yap Siew Qi is Consumables Technical Specialist at Shimadzu Asia Pacific. She joined the company after obtaining her PhD from National University of Singapore. At Shimadzu, she is responsible for the technical support of consumables in chromatography and spectroscopy. The products include GC/LC columns, UV cuvettes, vials and accessories. Her responsibilities include technical support of sales/engineers, customer support and producing applications.
</blockquote>

Shimadzu: LC Column Care & Troubleshooting

LC Column Care & Troubleshooting

Learn about ways to maintain LC column performance and prolong column lifetime.

Do you also wonder why there are more than 300 different types of C18 columns on the market?In this webinar, you will learn about the different stationary phases and columns for HPLC and when to use which one.Regarding the most well-known HPLC modes NP, RP, HILIC and Iex, we will focus on reversed phase columns as they are used in the majority of HPLC applications worldwide. In the end, you will get an understanding of why one C18 column is not like the other one.<blockquote>Presenter: Mareike Margraf (KNAUER expert for your column questions)</blockquote>

KNAUER HPLC columns for reversed phase 

In this webinar, you will learn about the different stationary phases and columns for HPLC and when to use which one. 

Anson Lewis, Global Sales Manager at Tosoh Bioscience, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. On April 8, Marissa Heino, Product Manager Process Solutions, and Abbie Breton, Application Development Manager at Tosoh Bioscience, will demonstrate the advantages of multi-column chromatography (MCC) technology.&nbsp;Batch purification leads to a substantial rise in expenses for end users, including costs of resin, buffers, and labor due to extended processing times. Advances in downstream mAb processing, such as multi-column chromatography systems, have proven effective in significantly lowering purification costs while increasing productivity. In this study, Marissa and Abbie highlight the advantages of MCC technology by showcasing a two-step purification platform using a benchtop MCC system to reduce adventitious viruses, with seamless scalability to production-scale MCC systems.&nbsp;<blockquote>Presenter: Anson Lewis (Global Sales Manager at Tosoh Bioscience)Presenter: Marissa Heino (Product Manager Process Solutions)Presenter:&nbsp;Abbie Breton (Application Development Manager at Tosoh Bioscience)</blockquote>

Tosoh Bioscience: Tosoh Tuesday - Intensified Batch Chromatography: A Viral Clearance Case Study

Tosoh Tuesday - Intensified Batch Chromatography: A Viral Clearance Case Study

Marissa Heino and Abbie Breton will demonstrate the advantages of multi-column chromatography (MCC) technology.

LCMS webinars focusing on Consumables - page 22

PRINCIPLES OF SIZE EXCLUSION CHROMATOGRAPHY: OPTIMIZING PARTICLE SIZE, PORE SIZE, AND COLUMN DIMENSIONS

Analytical BioSolutions for Therapeutic Oligonucleotides

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS

Harmonisation of Ph.Eur. 2.2.46 and USP 621

SEC-nHRMS for the Characterization of mAbs

Scale Up from Analytical to Prep with Agilent Prep LC Columns

Understanding LC Columns - Difference in Column Types and Phases

LC Column Care & Troubleshooting

Tosoh Tuesday - Intensified Batch Chromatography: A Viral Clearance Case Study

KNAUER HPLC columns for reversed phase

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