Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 30th September 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring to you applications by Shimadzu, Thermo Fisher Scientific, Waters Corporation, and Metrohm and a tech nical note by Agilent Technologies!<h4>1. Agilent Technologies: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_openlab_oligo_analysis_accelerator_5994_7798en_agilent_d8503d3d3c.pdf">Purity, Assay, and Impurity Profiling of Single-Stranded Oligonucleotides Using Agilent Oligo Analysis Accelerator for OpenLab CDS</a></h4><ul><li>Technical note</li></ul>IntroductionChallenges with therapeutic synthetic oligonucleotides include determining the sample purity, label claim or assay, and quantitation of impurities. Traditional HPLC methods that rely on 260 nm UV detection may not have the necessary sensitivity or specificity for quality control (QC) testing. Using a more selective detector such as a mass spectrometer is a viable approach for QC testing needs. Although LC/MS methods are a mainstay for characterization of oligos, many high-resolution accurate mass detectors may not have the robustness needed in a routine testing environment such as QC. Additionally, a high level of technical expertise is often required for MS operation, data analysis, and data interpretation.A more practical approach uses a single quadrupole detector that is both sufficiently selective and sensitive enough for purity, assay, and impurity profiling of oligos. Given its relatively high mass range and sensitivity in full scan mode, the Agilent InfinityLab LC/MSD XT can meet the demands for LC/MS QC.As demonstrated by Rentel et al.,1 the LC/MSD XT can be used and validated for the QC lot release of oligos. However, one challenge that remains is the complex, multistep data analysis which requires the extraction of several extracted ion chromatograms and manual integrations. Performing these operations in a chromatography data system (CDS) while meeting compliance requirements can be time consuming. Manual transcriptions by the analyst may lead to a high margin of error, further delaying decision making.This technical overview will address the Oligo Analysis Accelerator (OAA) for Agilent OpenLab CDS. This software add-on acts as the data analysis front end to OpenLab CDS, leveraging built-in technical controls that help meet data integrity and compliance requirements. By performing overlays, integrations, and calculations in a fit-for-purpose user interface, data analysis is significantly streamlined. This not only saves time-on-task but also improves data quality.SummaryThe final step in the workflow is to review the results. This includes the SST results shown in previous steps, as well as purity and identity results. Assay results may vary depending on which formulation was selected for the sample.It is important to note that the summary itself is not the report. An OpenLab CDS report template must still be used to report the results for review. Further, any electronic signatures and approvals occur within OpenLab CDS, not in OAA. Again, this is because OAA leverages the already existing data compliance engine within the OpenLab CDS platform.Future considerationsLC/MS data analytical workflows have been confined to data systems either used to interpret spectra or chromatograms. However, the need for more user-friendly, fit-for-purpose data analysis is becoming increasingly important for the development of complex therapeutics such as oligonucleotides.&nbsp;Agilent Oligo Analysis Accelerator for OpenLab CDS is a paradigm shift in the way software is being developed.<h4>2. Waters Corporation: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008495_en_8ac74493e3.pdf">Repeatability and Reproducibility of the Oasis™ WAX/GCB for PFAS Analysis Cartridges in Aqueous Samples for EPA Method 1633</a></h4><ul><li>Application</li></ul>AbstractIn January 2024, EPA Method 1633 was finalized. EPA Method 1633 is the first method to incorporate the determination of 40 PFAS compounds across many challenging environmental sample matrices outside of drinking water, including non-potable waters (ground water, surface water, and wastewater) as well as soil, biosolids, and tissue by LC-MS/MS analysis. EPA Method 1633 requires use of both weak anion exchange (WAX) solid phase extraction with graphitized carbon black (GCB) cleanup and is a performance-based method allowing for modifications as long as acceptance criteria for recoveries and %RSDs are met.This application note highlights the sample extraction for non-potable aqueous samples in EPA Method 1633 using a dual-phase, or bilayer cartridge: Oasis WAX/GCB for PFAS Analysis meeting the method acceptance criteria and displays reproducibility of the cartridges.Benefits<ul><li>Oasis WAX/GCB for PFAS Analysis, a dual-phase cartridge is reproducible and repeatable for EPA Method 1633 aqueous samples in both inter- and intra-batch assays</li><li>Reduction of manual steps, overall sample preparation time by use of the dual-phase SPE cartridge</li><li>Acceptance criteria for recoveries and %RSDs are met for EPA Method 1633 for aqueous samples</li></ul>ConclusionThis study demonstrates the reproducibility of the dual-phase Oasis WAX/GCB for PFAS Analysis cartridges for determination of 40 PFAS and standards using the ACQUITY UPLC I-Class System and Xevo TQ-XS Mass Spectrometer. The cartridges are suitable for PFAS analysis in accordance with EPA 1633 guidelines for recovery and %RSD. The WAX/GCB cartridges show excellent repeatability across multiple product lots and within multiple replicates of each lot across non-potable water samples. The data demonstrates Oasis WAX/GCB for PFAS Analysis cartridges are ideally suited for PFAS analysis from complex matrices, such as non-potable waters like those described in EPA Method 1633. Out of the box performance is expected lot to lot and within a lot for SPE when using Oasis WAX/GCB for PFAS Analysis.<h4>3. Thermo Fisher Scientific: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ab_003164_en_dllme_triplus_pfas_15478b8469.pdf">Dispersive liquid-liquid micro-extraction for the automated sample preparation of PFAS in drinking water</a></h4><ul><li>Application</li></ul>Application benefits<ul><li>Improved sensitivity: DLLME provides high enrichment factors, which can improve the detection sensitivity of PFAS in drinking water samples, down to challenging regulatory detection and reporting limits.</li><li>Cost-effective: DLLME uses a small volume of solvent, which not only reduces the cost but also makes the method more environmentally friendly.</li><li>Versatility: DLLME can be employed for the analysis of a wide range of PFAS classes, followed by either high performance liquid chromatography or gas chromatography as a separation technique, coupled to tandem mass spectrometry or high-resolution accurate mass as detection method.</li></ul>GoalTo demonstrate a comprehensive method for the analysis of per- and polyfluoroalkyl substances (PFAS) by harnessing the potential of automated sample preparation on the Thermo Scientific™ TriPlus™ RSH SMART liquid handling station, with dispersive liquid-liquid micro extraction (DLLME) as a simple, cost-effective, and versatile extraction and pre-concentration technique. This was performed on LC-MS, but the same workflow can be also applied to GC-MS.Keywords: DLLME, PFAS, sample preparation, automation, liquid handling, drinking waterConclusionsThe DLLME method presented in this work is a promising technique for the extraction and pre-concentration of PFAS from drinking water samples. Thanks to the described careful optimization and validation of the method, it provides an automated, fast, efficient, and green alternative to traditional and manual extraction methods. As compared to SPE procedures, the only manual steps consist in adding internal standards and an acidic solution to the sample, and then the TriPlus RSH SMART instrument will perform the extraction automatically, while for SPE procedures, the rest of the steps are mostly manual. This workflow allows laboratories to overcome the challenges associated with this analysis, providing high enrichment factors for all the different compound classes studied, as well as good reproducibility and robustness of the extraction process. In summary, the presented automated DLLME sample preparation has the following advantages:<ul><li>Reduced sample volume (15 mL) for easy sample handling, transportation, and storage.</li><li>Cost reduced by low solvent usage and no need for filters or SPE cartridges.</li><li>Automation enables reproducible and accurate results, with low inner- and cross-contamination.</li><li>Short runtime and automated process managed by Chromeleon software saves time and allows staff to perform more profitable activities.</li><li>The short runtime of the TriPlus RSH SMART instrument opens the possibility to feed one or more analytical instruments, including HPLC-MS or GC-MS technologies.</li><li>The extraction protocol without using filters and based on the use of low-density solvent for extraction can open the way for the extraction of other matrices such as wastewater.</li></ul><h4>4. Shimadzu: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00658_en_79d68e6421.pdf">Efficient Data Analysis with Peakintelligence - Application for Analyzing Per- and Polyfluoroalkyl Substances (PFAS)</a></h4><ul><li>Application</li></ul>User Benefits<ul><li>Peakintelligence peak integration software substantially reduces the time and effort required for data analysis in PFAS analysis.</li><li>Since it does not require parameter settings, Peakintelligence allows even inexperienced users to perform peak integration and achieve results on par with experienced workers.</li></ul>IntroductionPer- and polyfluoroalkyl substances (PFAS) are lyophobic, heat resistant, and chemically resistant compounds used extensively as coatings, surface treatment agents, emulsifiers, and fire-extinguishing agents. Concerns about PFAS and their persistence in the environment, accumulation in living organisms, toxicity to living organisms, and ability to travel long distances have recently led to fact-finding studies on PFAS and the introduction of regulations. As of October 2023, only three PFAS are subject to regulatory restrictions in Japan (PFOS, PFOA, and PFHxS), but more are subject to restrictions in the USA and Europe. Throughout the world, an increasing number of PFAS are expected to require analysis. These changes will create more analytical data that will have to be processed, creating a need for more efficient methods of data analysis.Peakintelligence is a peak integration software equipped with peak-finding algorithms that have been developed with the help of artificial intelligence (AI). This Application News describes a case example in which it was used to process chromatogram data of PFAS analysis and reduce the effort required and increase the efficiency of the analysis.ConclusionUsing Peakintelligence to process peaks in PFAS analysis substantially reduced the number of false detections and falsely identified peaks. It also significantly reduced the number of peaks that required manual processing, and it did not need pre-analysis adjustment of parameters. These improvements enable substantial reductions in the time and effort required to perform data analysis. Avoiding parameter settings by the user also eliminates variability in the results between users, which helps eliminate dependency on specific personnel. As the number of PFAS requiring analysis increases throughout the world, a more efficient method of analyzing peaks will be needed; Peakintelligence is an effective solution for this need.<h4>5. Metrohm: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_239_download_6884b899af.pdf">Iron speciation in LiFePO4 batteries</a></h4><ul><li>Application</li></ul><h5>Simultaneous determination of Fe(II) and Fe(III) in lithium iron phosphate with the Multi-Mode Electrode pro</h5>Lithium iron phosphate (also known as LiFePO4 or lfp) batteries last for over 2000 charges and are safer because of their lower risk of overheating. LiFePO4 batteries have a slightly lower energy density compared to lithium-ion batteries. However, high discharge rates of lfp batteries make them ideal for electric vehicles, renewable energy storage, and backup power systems. Lithium iron(II) phosphate is used as a cathode material in lithium iron phosphate batteries. Characterization of lfp and monitoring the oxidation state of iron in lfp batteries is relevant to battery performance in terms of durability, capacity, and safety. Additionally, analyzing chemical composition can be useful for battery research and can aid eco-friendly recycling practices. This is essential for driving battery technology forward and promoting clean energy solutions.Polarographic speciation of Fe(II) and Fe(III) can be used to evaluate the purity of LiFePO4 and its usability as a cathode material in lithium iron phosphate batteries. It can further be used to study the concentrations of Fe(II) and Fe(III) in the cathode material after several charging and discharging cycles to evaluate the aging behavior.

News from LabRulezLCMS Library - Week 40, 2024

What chemicals are we exposed to on a daily basis? That is the central question of ‘non-targeted analysis’ or NTA, an emerging field of analytical science that aims to identify all chemicals around us. A daunting task, because how can you be sure to detect everything if you don’t know exactly what you’re looking for? In a paper in Environmental Science and Technology, researchers at the Universities of Amsterdam (UvA, the Netherlands) and Queensland (UQ, Australia) assess this problem. In a meta-analysis of NTA results published over the past six years, they estimate that less than 2% of all chemicals have been identified.According to Viktoriia Turkina who performed the research as a PhD student with Dr Saer Samanipour&nbsp; at the UvA’s Van ‘t Hoff Institute for Molecular Sciences, this limitation underscores the urgent need for a more proactive approach to chemical monitoring and management. “We need to incorporate more data-driven strategies into our studies to be able to effectively protect the human and environmental health”, she says.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/University_of_Amsterdam_HIMS_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Viktoriia_Turkina_de5a1652e1.jpg" alt="University of Amsterdam / HIMS: Viktoriia Turkina." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_University_of_Amsterdam_HIMS_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Viktoriia_Turkina_de5a1652e1.jpg 164w," sizes="100vw" width="700" height="665">Samanipour explains that current monitoring of chemicals is rather limited since it’s expensive, time consuming, and requires specialized experts. “As an example, in the Netherlands we have one of the most sophisticated monitoring programs for chemicals known to be of concern to human health. Yet we target less than 1000 chemicals. There are far more chemicals out there that we don't know about.”<h4>A vast chemical space</h4>To deal with those chemicals, some 15 to 20 years ago the concept of non-targeted analysis was introduced to look at possible exposure in an unbiased manner. The idea is to take a sample from the environment (air, water, soil, sewer sludge) or the human body (hair, blood, etc ) and analyse it using well-established analytical techniques such as chromatography coupled with high resolution mass spectroscopy. The challenge then is to trace the obtained signal back to the structures of chemicals that may be present in the sample. This will include already known&nbsp; chemicals, but also chemicals of which the potential presence in the environment is yet unknown.In theory, this ‘chemical space’ includes as many as 1060 compounds, an incomprehensible number that exceeds the number of stars in the universe by far. On the other hand, the number of organic and inorganic substances published in the scientific literature and public databases is estimated at around 180 million. To make their research even more manageable, Turkina, Samanipour and co-workers focused on a subset of 60.000 well-described compounds from the NORMAN database. Turkina: “This served as the reference to establish what is covered in NTA studies, and more importantly, to develop an idea about what is being overlooked.”<blockquote>The vast ‘exposome’ of chemicals that humans are exposed to on a daily basis is a sign of our times, according to Samanipour. “These days we are soaking in a giant ocean of chemicals. The chemical industry is part of that, but also nature is running all a whole bunch of reactions that result in exposure. And we expose ourselves to chemicals by the stuff we use - think for instance of the problem of microplastics. To solve all this we have to be able to go beyond pointing fingers. With our research, we hope to contribute to finding a solution together. Because we all are in the same boat.”</blockquote><div><div><div><div><h4>Much room for improvement</h4>The meta analysis, which included 57 NTA papers, revealed that only around 2% of the estimated chemical space was covered. This can indicate that the actual exposure to chemicals is indeed quite low, however, it can also point to shortcomings in the applied analyses. According to Turkina and Samanipour, the latter is indeed the case. They focused on NTA studies applying liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) -one of the most comprehensive methods for the analysis of complex environmental and biological samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/University_of_Amsterdam_HIMS_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Saer_Samanipour_5c78a79b5b.jpg" alt="University of Amsterdam / HIMS: Saer Samanipour." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_University_of_Amsterdam_HIMS_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Saer_Samanipour_5c78a79b5b.jpg 180w," sizes="100vw" width="700" height="606"><div>It turned out that there was much room for improvement. For instance in sample preparation, they observed a bias towards specific compounds rather than capturing a more diverse set of chemicals. They also observed poor selection and inconsistent reporting of LC-HRMS parameters and data acquisition methods. “In general”, Samanipour says, “the chemical analysis community is to a great extent driven by the available technology that vendors have developed for specific analysis purposes. Thus the instrumental set-up and data processing methods are rather limited when it comes to non-targeted analysis.”To Samanipour, the NTA approach is definitely worth pursuing. “But we need to develop it further and push it forward. Together with vendors we can develop new powerful and more versatile analytical technologies, as well as effective data analysis protocols.” He also advocates a data-driven approach were the theoretical chemical space is ‘back calculated’&nbsp; towards a subset of chemicals that are highly likely to be present in our environment. “Basically we have to better understand what is the true chemical space of exposure. And once those boundaries are defined, then it becomes a lot easier to assess that number of 2% we have determined.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/University_of_Amsterdam_HIMS_EST_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Distribution_of_all_chemicals_found_in_the_reviewed_articles_orange_overlaid_on_top_6a16bff0b0.jpg" alt="University of Amsterdam / HIMS / EST: Distribution of all chemicals found in the reviewed articles (orange), overlaid on top." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_University_of_Amsterdam_HIMS_EST_There_is_much_to_improve_in_identifying_all_the_chemicals_around_us_Distribution_of_all_chemicals_found_in_the_reviewed_articles_orange_overlaid_on_top_6a16bff0b0.jpg 245w," sizes="100vw" width="777" height="441"></div></div></div></div></div>

There is much to improve in identifying all the chemicals around us 

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<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 5</h4>
Analytical Method Development
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<ul>
<li>How to choose the right Analytical Column Kit</li>
<li>Select the critical Parameters for your Analytical Methods</li>
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Prezenter: Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)
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Andrea has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab position, and technical application support manager. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications.
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Prezenter: Tobias Langrock (Senior Application Specialist Chemistry Europe, Waters)
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During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.
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Back to Basics - Sample Preparation: Theory and Overview
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Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple
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Analytical Method Development

How to select analytical column kit and the critical parameters for your method.

Analytical Method Development (HPLC, LC/MS, columns)

Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
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<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
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<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
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<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
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<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
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<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
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<h5>Talk 1: Advanced Analytical Solutions for Nucleic Acid Analysis and Purification</h5>
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<li>Presented by Dr. Charlotte Lekieffre, Biopharma Application Specialist, Agilent Technologies</li>
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The emphasis during the second day of the Summit will be on advanced analytical solutions, such as HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Additionally, you’ll discover techniques to enhance the efficiency and accuracy of your processes by scaling up oligonucleotide purification using Agilent biocolumns. 
<h5>Talk 2: Extracted Yeast tRNA Sequencing by LC-MS/MS</h5>
<ul>
<li>Presented by Aurore Attina, Engineer of Mass Spectrometry, University of Montpellier</li>
</ul>
RNA modifications are essential for RNA stability, maturation, and functionality; directly influencing protein translation and gene regulation. They play a central role in various biological processes and are associated with many diseases, including cancers and neurological disorders. tRNA (transfer RNA), which plays a key role in protein synthesis, is the most modified molecule in the organism known to date. tRNA modifications are essential to ensure their correct function in protein translation, maintain the accuracy and fidelity of the genetic code, and enable flexible adaptation to changing cellular conditions and are therefore a crucial attribute to monitor. To monitor and map these modifications of tRNA, we have developed a LC-HRMS analytical workflow, first using synthetic oligonucleotide for the method optimization. In this webinar, we will first go through the various steps involved in LC-MS/MS method optimization using synthetic oligonucleotides. In a second part, we will present the protocol development of an enzymatic digestion applied to tRNA extracted from yeast. This digestion generates oligonucleotide fragments that can be analyzed by LC-MS/MS for sequence confirmation.
<h5>Talk 3: Discover Agilent’s Software Tools to Confirm Oligonucleotide Sequence Integrity</h5>
<ul>
<li>Presented by Dr. Holger Stalz, LC/MS Product Specialist Austria &amp; Switzerland, Agilent Technologies</li>
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Mass spectrometry (MS) is increasingly important for the development of therapeutic modalities such as oligonucleotides. A common analytical method for large molecules is intact mass by LC/MS. This can be used for the characterization and identification of a product or process-related impurities, or simply as a confirmation of molecular weight. The newly introduced Agilent OpenLab CDS 2.8 SW version comes with a set of features to enable the automatization of biopharma workflows. If you want to know how LC/MSD Spectral Deconvolution of your biopharma samples can be automated, we will provide answers during our presentation. Even more details on Oligonucleotide sequence confirmation and impurities characterization can be acquired using the power of a high-resolution accurate mass system (HRAM) on the Agilent 6545XT AdvanceBio LC/Q-TOF. Analyzing the sample data with the software workflows in Agilent BioConfirm version 12 will support the user to get the trusted answers to their most relevant questions.
<h5>Talk 4 Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns</h5>
<ul>
<li>Presented by Dr. Andrea Tripodi, Application Development Engineer, Agilent Technologies</li>
</ul>
Several classes of nucleic acids, such as antisense oligonucleotides, small interfering RNAs (siRNAs), and aptamers are being investigated for potential therapeutic applications. Synthetic oligonucleotides inevitably contain many impurities due to limitations in the efficiency of each chemical reaction used during manufacture. They include deletion sequences (missing one or more nucleotides) and reaction by-products often arising during the final deprotection or cleavage steps. Ion pair reversed-phase (IP-RP) chromatography stands out as the prevalent method for analyzing and purifying oligonucleotides. For increased resolution and separation of your full-length synthetic oligonucleotide from closely related impurities, AdvanceBio Oligonucleotide is the ideal choice. The latest developments include semi-preparative, and lab-scale preparative columns packed with superficially porous particles that provide excellent performance and resolution without compromising column loading. A fast and easy scale-up method from analytical to semi-preparative scale was successfully achieved using a 10 mm ID column enabling higher sample loading using the same analytical LC instrument.

Technology Networks: Biopharma Innovations Summit

Biopharma Innovations Summit Day 2

Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development.

In the second part of the stationary phases webinars, we look at other separation modes: IEX, SEC, ligand exchange and HILIC and what we think they are best suited for.<blockquote>Presenter: Cecilia Mazza (Product Specialist, EMEA IDO - Chemistries &amp; Suppliers, Agilent)With more than 20 years of professional experience, Cecilia is presently the Product Specialist for columns and supplies at Agilent. Supporting distributors and laboratory users in the analysis and purification of small and large molecules, for a wide range of LC and GC applications as well as sample preparation.Cecilia holds a Ph.D. in Chemical Engineering from Rensselaer Polytechnic Institute in US.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Stationary Phases in HPLC - Part II

We look at other separation modes: IEX, SEC, ligand exchange and HILIC and what we think they are best suited for.

In this part of the course we will deal with sample preparation: why, how and which sample preparation is the most suitable for the target analyte?SPE, LLSE, or syringe filter?&nbsp;<blockquote>Presenter: Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</blockquote>

Sample Preparation in HPLC

Why, how and which sample preparation is the most suitable for the target analyte?

Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting.After the session, we will be able to identify causes and avoid errors.<blockquote>Giorgio Ferlat (MSc, EMEAI IDO Product Specialist, Chemistries and Supplies, Agilent Technologies)Giorgio achieved his Master Degree in Chemistry at the University of Trieste, Italy and has over 25 years of analytical chemistry experience, both in the laboratory and in the field, with strong expertise.He has over 15 years of experience in consultative consumable sales and business development covering both national and international positions.</blockquote>

Troubleshooting and Everyday Routine (HPLC Columns)

Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting. 

Per- and Polyfluoroalkyl Substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications that include more than 12,000 different compounds.Due to the persistent nature of these compounds, as well as their ability to be easily transported through the environment, there is a significant concern for the presence of PFAS in the human body. A LC-MS/MS method utilizing microelution SPE for sample cleanup, presented for analysis of seven PFAS, identified significant by National Academies of Sciences, Engineering, and Medicine for clinical research and biomonitoring in public health labs.&nbsp;<h4>Who Should Attend:</h4><ul><li>Any scientists interested in LC-MS/MS and sample preparation or anyone curious about PFAS from biological samples</li></ul><h4>Key Learning Points:</h4><ul><li>Learn key steps to LC-MS/MS and sample preparation of biological samples</li><li>Understand potential Challenges when extracting PFAS from biological samples</li><li>Find advantages of using Micro elution SPE sorbent technology</li></ul><blockquote>Presenter: Shahana W. Huq (Senior Application Scientist, Phenomenex)Shahana is a Senior Application Scientist focusing on emerging technologies and solutions within Clinical Research and Sample Preparation, at the Phenomenex Applications Laboratory since the year 2000. She holds an MS in Biochemistry from California State University, Los Angeles. Her work involves experimental design, analytical method development, optimization, and troubleshooting. &nbsp;Furthermore, she has extensive expertise in R&amp;D product innovation and application development, as well as hands-on experience in sample preparation and LC-MS/MS. Shahana’s drive, and vision of the clinical field has motivated her to participate in various scientific publications and even as a coauthor of Phenomenex patent on sample preparation products. Currently, Shahana works closely with global customers in the applied market, providing technical support and consultation services as well as onsite seminars and webinars to promote peer development. &nbsp;Presenter: Richard Jack, PhD (Global Market Development Manager, Phenomenex)Richard is the global market development manager for the environmental and food markets at Phenomenex. He has over 18 years of experience with chromatography and mass spectrometry for the environmental, food, cannabis, semiconductor, chemical, and pharmaceutical industries. Jack has collaborated with global regulatory agencies to develop validated methods through new applications, instrumentation, column chemistries, and software. He is a former EPA scientific advisor for the EPA’s panel on hydraulic fracturing and a coauthor for EPA 557 and 557.1, ASTM D8001, and updates to D4327 and D6919 methods. He is currently the second vice chairman for the ASTM D19 subcommittee on water analysis. Previously, Jack was a vertical marketing manager with Thermo Fisher Scientific, Dionex, and Hitachi High Technologies, where he designed analytical instrumentation, including IC and HPLC systems, pumps, autosamplers, and a variety of detectors. He received his PhD in biochemistry and anaerobic microbiology from Virginia Tech in Blacksburg, Virginia. He received his master’s degree in ecology from the University of Tennessee in Knoxville.</blockquote>

Phenomenex: LC-MS/MS Analysis of PFAS in Bovine Serum for Clinical Research Using Microelution SPE

LC-MS/MS Analysis of PFAS in Bovine Serum for Clinical Research Using Microelution SPE

A LC-MS/MS method utilizing microelution SPE for sample cleanup, presented for analysis of seven PFAS.

LC-MS/MS Analysis of PFAS in Bovine Serum

The allowable adjustments to compendial methods published in USP General Chapter 621 and Ph. Eur. 2.2.46 provide scope for analysts to migrate methods to smaller column dimensions, providing advantages in terms of time and cost savings.This webinar will provide insight into the considerations necessary when applying allowable adjustments to gradient monographs.<h4>Key Learning Points:</h4><ul><li>Learn about Allowable adjustments possible when working with gradient monograph methods</li><li>Understand system considerations in terms of dwell volume</li><li>Dig into calculations to perform in order to scale gradient conditions correctly</li></ul><h4>Who Should Attend:</h4><ul><li>Any analysts working with pharmacopeia methods or chromatographers who work with gradient methods&nbsp;</li></ul><blockquote>Presenter: James Turner (Global Market Manager, Phenomenex)With more than 25 years of experience at Phenomenex, James has been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various customer problems. James has also been involved with the technical training of many Phenomenex technical consultants, across the multiple techniques we serve.</blockquote>

Phenomenex: Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Insight into the considerations necessary when applying allowable adjustments to gradient monographs.

Working with Allowed Adjustments Pharmacopeia HPLC

This comprehensive webinar will equip you with the knowledge and skills needed to excel in N-glycan analysis, from sample preparation to data interpretation.Enhance your understanding of the latest tools Agilent offers to address your biotherapeutic characterization needs.<h4>By attending this webinar, you will:</h4><ul><li>Understand the significance of N-glycans &nbsp;</li><li>Learn about the latest N-glycan sample preparation techniques &nbsp;</li><li>Understand how to select the right columns and perform HILIC separations &nbsp;</li><li>Analyze data with the new Glycan Peak Assignment Tool</li></ul><blockquote>Presenter: Tom Rice (Bioconsumables Product Manager, Agilent Technologies)Tom Rice is the Bioconsumables Product Manager at Agilent Technologies, coming to Agilent through the acquisition of ProZyme in 2018. Prior to joining Agilent, he held various sales and marketing roles within ProZyme beginning in 2014. Much of Tom’s work, both at ProZyme and Agilent, has been focused on bringing innovative glycobiology solutions to biopharma customers. Tom is based in California and holds a B.S. from Sonoma State University.Presenter:&nbsp;Randall Robinson (Research and Development Scientist, Chemistries and Supplies Division at Agilent Technologies)Randall Robinson is a Research and Development Scientist in the Chemistries and Supplies Division at Agilent. Prior to joining Agilent in 2021, Randall completed his PhD in food chemistry with a focus on developing analytical techniques to characterize bioactive oligosaccharides in milk and dairy streams. Since joining Agilent, he has contributed to the design of numerous products utilized for N-glycan analysis, including the HILIC column described in this presentation.</blockquote>

Separation Science: Streamlining N-Glycan Analysis: A Complete Workflow from Agilent

Streamlining N-Glycan Analysis: A Complete Workflow from Agilent

Learn about the latest N-Glycan sample preparation techniques.

Streamlining N-Glycan Analysis: A Complete Workflow

PFAS testing has become one of the most important topics facing food and environmental laboratories over the last two years.&nbsp;Phenomenex and SCIEX have worked with collaborators to overcome the significant challenges of such analyses, and now offer extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.<h4>Key Learning Points</h4><ul><li>Background to PFAS and their regulations</li><li>System Settings and requirements</li><li>Correct HPLC column choice</li><li>Sample Preparation strategies from various matrices</li><li>PFAS as ubiquitous contaminants - Inerting the system</li><li>A sensitive and robust method applicable to water and food sample</li><li>Some insight about designed for PFAS products</li></ul><h4>Who should attend</h4><ul><li>LC/MS or Sample Prep Users, working with several matrices that want to get tips on PFAS method development or on improving their method.</li></ul><blockquote>Presenter: Jianru Stahl-Zeng, Dr.&nbsp; (Senior Technical Marketing Manager EMEAI, SCIEX)Jianru has been part of SCIEX since 2001. She initially was part of our application Support team and covered not only proteomics but also small molecules applications. Jianru moved to technical marketing and focused on Food and Environmental applications.Since 2018, she is managing the EMEAI technical marketing team and projects. Jianru is also the Global Technical Leader for Food and &nbsp;Environmental application.Presenter:&nbsp;Duilio Romanello (Senior Technical Specialist, Phenomenex)Duilio has been working with Phenomenex since 2008. Since 2010 he has been Product Specialist for the GC and SPE lines and then Account Manager for the South of Italy. Since November 2023 he is covering the role of Senior Technical Specialist.He has acquired experience in the Food and Environmental sectors by collaborating with important companies in the industry and providing technical support for the development of method optimization, guidance on applications and troubleshooting, assistance in choosing columns for HPLC, Gas Chromatography (GC), SFC and SPE.</blockquote>

Phenomenex: Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.

Advanced strategies in LC/MS/MS for the clean-up

<h4>Overview</h4>We invite you to our 2nd Annual Virtual Biosymposium. This year, we will explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA, along with key method development considerations with global thought leaders.We look forward to seeing you! Key Event Objectives:<ul><li>Learn about novel approaches to characterizing established and modern biotherapeutic modalities</li><li>Understand key considerations when developing characterizing and quantitating methods for biomolecules</li><li>Take a deep dive into the values of fit-for-purpose methods to make critical drug decisions</li></ul>AGENDA7:30am AM (PT) - Welcoming Introductions<ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul>7:35 AM (PT) - "Solutions &amp; Characterization Strategies for IVT mRNA Therapy Development"<ul><li>Charles Bai, Lead Application Scientist, VectorBuilder, Chicago, USA</li></ul>8:05 AM (PT) - &nbsp;"Utilizing Size Exclusion Chromatography for the Quality Control Analysis of Gene Therapeutics”<ul><li>Rebecca Hirschberger, QC Manager Bioanalytical Implementation, and Dr. Christopher Gawlig, QC Manager Research and development, Biospring, Germany</li></ul>8:35 AM (PT) "Impurity Profiling Optimization of GalNAc-conjugated Oligonucleotides by LC-MS-UV"<ul><li>Roxana Eggleston-Rangel, Senior Applications Scientist, Phenomenex, California, USA</li></ul>9:05 AM (PT) – Round Table Q&amp;A<ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul>9:25 AM (PT) – Closing Remarks<blockquote>Presenter: Charles Bai (Lead Application Scientist)Charles has received his PhD in Microbiology at Purdue University. As a Lead Application Scientist at VectorBuilder, his main responsibilities are to provide technical consultation for clients regarding gene delivery using viral and non-viral vectors. Regarding the application of in vitro transcribed (IVT) RNA, Charles has been helping researchers with the design, production and encapsulation of IVT mRNA, circRNA or saRNA for therapy and vaccine development.Presenter: Rebecca Hirschberger (Quality Control Bioanalytical Method Development)Rebecca has been in the chromatography industry for over 2 years and has practical experience in the quality control of gene therapeutics. She has earnt a Masters in pharmaceutical science from Ludwig-Maximilians University, Munich, focused on instrumental analytics. Since Dezember 2022 she is a licensed Pharmacist in Germany. Rebecca Hirschberger joined BioSpring in August of 2021 and she serves as QC Manager Bioanalytical Implementation. Her area of responsibility includes: Various analyses of oligonucleotides, including (tandem) mass spectrometry via qToF, biochemical techniques, i.e. ELISA, enzymatic transformation of oligonucleotides for analytical and quality control purposes and the Separation of oligonucleotides by chromatographic methods.Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)Roxana joined Phenomenex in 2018 as an Applications Scientist and is currently working with the applications team to produce analytical solutions for scientist in the biopharma industry. Roxana’s current work focuses on characterization of mRNA, sgRNA and siRNA. Prior to Phenomenex, Roxana worked for Caltech and the University of Southern California developing LM-CS workflows for the proteomics community. Roxana Eggleston-Rangel received her bachelor’s and master’s degree in Chemistry and Biochemistry from California State University, Los Angeles.</blockquote>

Phenomenex: 2nd Annual Virtual Biosymposium

2nd Annual Virtual Biosymposium

Explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA

LCMS webinars focusing on Consumables | LabRulez LCMS