<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Thermo Fisher Scientific

Waters Corporation

<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 4</h4>
Basics of Liquid Chromatography
What will you learn?
<ul>
<li>LC System Components: Pumps and Injectors</li>
<li>LC Detectors: Which one is right for your Application?</li>
</ul>
<blockquote>
Prezenter: Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)
</blockquote>
<blockquote>
Andrea has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab position, and technical application support manager. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications.
</blockquote>
<blockquote>
Prezenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)
</blockquote>
<blockquote>
Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs.
</blockquote>
<blockquote>
Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.
</blockquote>
<h4>What&#39;s next?</h4>
Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652">here</a>
Back to Basics - Sample Preparation: Theory and Overview
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Theory of Liquid Chromatography
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Basics of Liquid Chromatography
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Analytical Method Development
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - The importance of Compliance
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Introduction to Mass Spectrometry
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
Back to Basics - Troubleshooting your LC and MS Systems
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
Not sure to be able to attend live sessions?
Register today and watch the webinars on-demand

Waters Corporation: Back to Bacisc Free Educational Webinar Serries

Basics of Liquid Chromatography

Intro to LC system components: pumps, injectors, and detectors.

Event Overview:
<ul>
<li>Principle of Chirality
</li>
<li>Chiral Stationary Phases (CSP)
</li>
<li>Polysaccharide CSPs and their Chiral Retention Mechanisms
</li>
<li>Chiral Method Development: Separation Modes and Screening Protocol
</li>
<li>SFC Chiral Method Development
</li>
<li>Chiral Method Optimization: Case Study
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who would like to understand how chiral separations work and how to apply the principles of chiral theory in developing an analytical method for separation of enantiomers.
</li>
<li>Any analyst who wishes to learn the basics of chiral theory.
</li>
<li>Any academic or pharmaceutical scientists who need to develop a method for separation and characterization of chiral compounds.
</li>
</ul>
Key Learning Points:
<ul>
<li>Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are
</li>
<li>Understand the three critical components of Polysaccharide CSPs and review the difference between coated and immobilized CSPs and how they are applied in different analytical contexts
</li>
<li>Learn how to approach a chiral screen using different separation modes.
</li>
</ul>
<blockquote>
Presenter: Laura K Ward (Technical Specialist, Phenomenex)
</blockquote>
<blockquote>
Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
</blockquote>

Phenomenex: LC Chiral Theory and Method Development

LC Chiral Theory and Method Development

Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are.

As part of the global effort to reduce CO₂ emissions, biofuels are becoming an increasingly important source of green energy.
Quality control testing of biofuels is therefore crucial to ensure the efficiency and reliability of biogas, biodiesel, and fuel ethanol. 
In this webinar you will learn about the latest laboratory testing techniques that are used to ensure the quality, efficiency, and sustainability of modern biofuels to enable producers to confidently meet international regulatory requirements and to promote their widespread adoption in both industry and the wider society at large. 
Experts from Agilent Technologies will introduce a wide range of analytical testing solutions covering multiple technologies that are routinely used to detect organic and inorganic impurities in biodiesel and enable the stringent quality control of fuel ethanol, biogas and biogas blends to be achieved. The expert panel will be available to answer questions from the audience at the end of the presentation. 
<blockquote>
Presenter: Danny van Oevelen (LC Product Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Luca Godina (GC &amp; GC/MS Application Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Sebastien Sannac (Spécialiste produit spectroscopie atomique, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Donald Inglis (Product Specialist EMEAI for FTIR, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Remko van Loon (GC &amp; GC/MS Product Specialist, Agilent Technologies)
</blockquote>

Agilent Technologies: Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

Learn about the latest laboratory testing techniques that are used to ensure the quality, efficiency, and sustainability of modern biofuels.

Comprehensive Quality Control Testing of Biofuels

Learn how Light Scattering can benefit your drug development!
The scientific community aims to enhance transparency and reliability in published research, with a specific focus on the quality of biological reagents, notably recombinant proteins. Concerns have been raised regarding irreproducible results, prompting discussions on common quality issues associated with recombinant proteins and their impact on downstream applications. In response, professionals in protein purification and characterization have collaboratively developed guidelines for improved quality control.
These guidelines include minimal information requirements for protein identity, production parameters, and long-term stability, as well as a minimal set of quality tests for purity, homogeneity, and identity. Additional recommendations cover DNA contamination, “spectral and thermal denaturation signatures”, homogeneity, “competent fraction”, storage conditions, batch-to-batch reproducibility, and other factors based on intended applications. 
Evaluation of these guidelines over a one-year period suggests that their implementation can enhance experimental reliability and optimize protein quality. Investing in protein QC benefits all stakeholders in life sciences, including researchers, editors, and funding agencies, by improving data accuracy and minimizing resource wastage
Key learning topics:
<ul>
<li>The latest guidelines for Quality control of Protein purification and characterisation 
</li>
<li>How these guidelines can enhance reliability and improve protein quality in your labs
</li>
<li>How to reduce wastage and improve data accuracy in protein quality control
</li>
</ul>
Who should attend?
<ul>
<li>Lab Managers and Scientists working in upstream recombinant protein production and QC
</li>
<li>Lab Managers and Scientists working in downstream protein purification QC
</li>
</ul>
<blockquote>
Presenter: Dr. André Matagne (Full Professor, University of Liège, Belgium)
</blockquote>
<blockquote>
Dr. Matange is a Full Professor at the University of Liège, Belgium, where he is the chair at the Centre for Protein Engineering within the Faculty of Sciences.
</blockquote>
<blockquote>
With a background in biochemistry, his research is primarily focused on proteins, particularly exploring their folding, stability, and biological activity. Notably, he has played a pivotal role in the development of Robotein®, an advanced technological platform designed for to high throughput protein expression, purification, formulation, and analysis.
</blockquote>
<blockquote>
Since 2016, Dr.Matagnehas been honored as an elected titular member of the Royal Academy of Sciences in Belgium.
</blockquote>

Waters Corporation: Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results​

Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results​

Learn how Light Scattering can benefit your drug development.

Quality Control of Purified Proteins to Improve Research

<h4>Live from the Lab hosted by NIBRT: Solutions for biotherapeutic analysis</h4>
By your side to accelerate your biopharmaceutical development journey
Join us for the second Live from the Lab of 2024, taking place on September 17th, an exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry. Focusing on all stages of biotherapeutics development, from discovery and research, all the way to routine and QC analysis, this event will give you valuable insights to accelerate your biopharmaceutical development journey.
The following speakers will talk at this event at the NIBRT research facility which will be streamed live all over Europe:
<ul>
<li>Dr. Jonathan Bones, NIBRT</li>
<li>Prof. Mark Dickman, University of Sheffield</li>
<li>Dr. Dan Bach Kristensen, Symphogen</li>
<li>Dr. Mark Hilliard, Pfizer</li>
<li>Dr. Ken Cook, Thermo Fisher Scientific</li>
<li>Jon Bardsley, Thermo Fisher Scientific</li>
</ul>
Whether you are a researcher, scientist, or industry professional in our region, this biopharma event is not to be missed. Expand your knowledge, network with experts, and discover how the Thermo Scientific solutions will stand by your side to accelerate your biopharmaceutical development journey.
Mark your calendars for September 17th and join us for this impactful hybrid event foster collaboration within the scientific community.
Participation is free of charge, registration is required.
For any questions about this event or regarding your registration, please email CMDEvents@thermofisher.com.
<h4>How to attend?</h4>
3 ways of attending:
<h5>Online attendees (remote):</h5>
Unable to travel to a satellite? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from NIBRT.
<ul>
<li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/Flyers/fl-003119-pb-live-from-the-lab-ms-biotherapeutics-agenda-online-fl003119-em-en.pdf">Agenda</a></li>
</ul>
<h5>NIBRT Research Facility (in-person event):</h5>
Join the heart of the event where the main agenda will be hosted and live streamed across our region to our satellite events and our online audience. Attendance through VIP invitation only!
<h5>Join a ‘Satellite’ location (in-person event):</h5>
Across Europe, our in-person satellite events will be broadcasting the main agenda live streamed from NIBRT, Dublin and supplement the day with local speakers/lab tours pending on the satellite. Take this opportunity to network with fellow scientists and meet your Thermo Fisher representatives from technical, commercial, and service functions. Check out the closest location to you and their individual agenda!
<ul>
<li>Søborg, Denmark (AGC Biologics)</li>
<li>Dreieich, Germany (Thermo Fisher Scientific)</li>
<li>Segrate, Italy (MI) (Thermo Fisher Scientific S.P.A.)</li>
<li>Leiden, Netherlands (Janssen Biologics BV)</li>
<li>Ljubljana, Slovenia (Four Points by Sheraton Ljubljana Mons)</li>
<li>Reinach, Switzerland (Thermo Fisher Scientific)</li>
<li>Hemel Hempstead, UK (Thermo Fisher Scientific)</li>
</ul>

Thermo Fisher Scientific: Live from the Lab: Solutions for biotherapeutics

Live from the Lab: Solutions for biotherapeutics

An exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry.

The sample throughput of high-sensitivity liquid chromatography-mass spectrometry (LC-MS) based proteomics analysis is restricted by the pressure limitations of LC and column hardware, with some methods wasting as much as 50% of the method cycle time outside of MS acquisition.
Traditional routes for boosting throughput include shorter columns and gradients, higher flow rates, and using trap-and-elute workflows – all of which introduce trade-offs in sensitivity and proteome depth.
The Thermo Scientific™ Vanquish™ Neo tandem direct injection workflow leverages hardware innovations and software intelligence to eliminate method overhead while maintaining data quality across nano, capillary, and micro-flow LC-MS.
In this webinar experts from Evotec and Bayer will showcase how they implemented the tandem direct injection workflow for their proteomics work. Dr. Nagarjuna Nagaraj will present on how Evotec is using tandem direct injection with nano-LC columns packed in-house to boost sample throughput of their chemoproteomics screening pipeline, while Dr. Alexander Boychenko from Bayer will discuss how his lab is using a micro-flow tandem direct injection approach to analyze plasma samples for translational proteomics.
Learning Objectives
<ul>
<li>See how minimizing LC-MS method overhead can accelerate sample throughput while maintaining proteome depth
</li>
<li>Discover how labs are using tandem direct injection for chemoproteomics and translational proteomics applications
</li>
<li>Learn how to implement tandem direct injection in your proteomics workflow
</li>
</ul>
<blockquote>
Presenter: Dr. Nagarjuna Nagaraj (Group Leader High Sensitivity Proteomics, Evotec)
</blockquote>
<blockquote>
Dr. Nagarjuna Nagaraj is a proteomics group leader at Evotec focusing on high sensitivity and high throughput proteomics applications with more than 18 years of experience in proteomics and mass spectrometry. Nagarjuna obtained his PhD from the Matthias Mann lab focusing on sample preparation and MS data acquisition strategies for clinical applications. After a postdoc he directed the mass spectrometry core facility at the Max-Planck-Institute of Biochemistry working on a wide range of protein and peptide mass spectrometry projects such as bottom-up proteomics, structural mass spectrometry including HDX, native and crosslinking MS, intact protein and small molecule analysis.
</blockquote>
<blockquote>
Presenter: Dr. Alexander Boychenko (Head of R&amp;D Laboratory Precision Medicine Markers, Bayer)
</blockquote>
<blockquote>
Alexander Boychenko is leading Omics Research within the Translational Sciences team of the Pharmaceuticals division at Bayer Group. He is focused on implementing innovative analytical technologies and developing novel methodologies for proteomics, metabolomics, and ligand-binding assays (LBA) to comprehensively characterize human and animal model phenotypes, enabling disease understanding and novel biomarker discovery. He holds a Ph.D. in Analytical Chemistry from Kharkiv National University, Ukraine. During his post-doctoral work at the University of Groningen, The Netherlands, he combined LC-MS and immunoassays to discover and validate protein biomarkers in biofluids for cancer screening. Before joining Research and Early Development at Bayer AG, he developed and introduced new liquid chromatography-mass spectrometry products at Thermo Fisher Scientific. Alexander is a subject matter expert in LC-MS, biological data interpretation, and precision medicine. He utilizes his passion for applying technical expertise to develop new medicines.
</blockquote>
<blockquote>
Presenter: Dr. Alec Valenta (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Alec is the product marketing manager for the Vanquish Neo UHPLC system and low-flow LC columns. Prior to joining Thermo Fisher Scientific he earned his PhD in analytical chemistry from the University of Michigan where he helped advance microdialysis sampling of neurochemicals and developed LC-MS methods to quantify small molecule neurotransmitters, neuropeptides, and metabolites in a variety of complex biological samples.
</blockquote>

Thermo Scientific: A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

How experts from Evotec and Bayer implemented the tandem direct injection workflow for their proteomics work.

A Tandem LC Workflow for Maximizing Mass Spectrometer

<div class="summary-section section">This webinar will introduce liquid chromatography and provide a comprehensive overview of an HPLC system, helping new users understand the purpose of each component. It will also explore some common chromatographic terms, what they mean to the separation, and how understanding these terms can help analysts make better decisions. Finally, we will discuss the attributes of chromatographic columns and how they impact the separation process.<h5>Key Learnings:</h5>1. HPLC system overview2. Key chromatographic terms3. Chromatography overview4. Understanding the chromatographic column&nbsp;<div style="clear:both;">Who should attend: Analysts who are brand new to HPLC or those who need a strong understanding of the principal concepts of HPLC.</div><div style="clear:both;">&nbsp;</div><blockquote><div>Presenter: Maureen DeLoffi (Senior Product Manager, Waters Corporation)</div><div> Maureen DeLoffi is a Senior Product Manager at Waters Corporation, where she manages the small molecule portfolio of Analytical HPLC columns. Prior to her Product Manager role, she spent over 12 years as a scientist in the Column Technology Development Lab at Waters. She holds a bachelor’s degree in Mechanical Engineering from the University of New Hampshire.</div></blockquote></div>

Waters Corporation: Fundamentals of HPLC Separations for Small Molecule Analyses

Fundamentals of HPLC Separations for Small Molecule Analyses

This webinar will introduce liquid chromatography and provide a comprehensive overview of an HPLC system.

We start the LC Beginner webinar series with an overview of basic terms relevant to liquid chromatography.&nbsp;<blockquote>Presenter: Laura Montis (Product Specialist Liquid Phase Separations, Agilent)Laura began her carrier in the Liquid Chromatography over 15 years ago. After experiencing the pharmaceutical world in an analytical development lab, she went on to work with preparative chromatography and gained experience with Supercritical Fluid Chromatography prior to moving onto special HPLC applications, like 2DLC and Online LC.Throughout her career, Laura has held several positions, including application chemist, trainer, sales specialist, and marketing business development. She is currently Product Specialist for liquid phase chromatography systems within the International Distributor Organization in EMEA at Agilent Technologies.Laura studied analytical chemistry and holds a master’s degree from University of Lyon in France.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Liquid Chromatography Fundamentals

We start the LC Beginner webinar series with an overview of basic terms relevant to liquid chromatography. 

The allowable adjustments to compendial methods published in USP General Chapter 621 and Ph. Eur. 2.2.46 provide scope for analysts to migrate methods to smaller column dimensions, providing advantages in terms of time and cost savings.This webinar will provide insight into the considerations necessary when applying allowable adjustments to gradient monographs.<h4>Key Learning Points:</h4><ul><li>Learn about Allowable adjustments possible when working with gradient monograph methods</li><li>Understand system considerations in terms of dwell volume</li><li>Dig into calculations to perform in order to scale gradient conditions correctly</li></ul><h4>Who Should Attend:</h4><ul><li>Any analysts working with pharmacopeia methods or chromatographers who work with gradient methods&nbsp;</li></ul><blockquote>Presenter: James Turner (Global Market Manager, Phenomenex)With more than 25 years of experience at Phenomenex, James has been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various customer problems. James has also been involved with the technical training of many Phenomenex technical consultants, across the multiple techniques we serve.</blockquote>

Phenomenex: Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Insight into the considerations necessary when applying allowable adjustments to gradient monographs.

Working with Allowed Adjustments Pharmacopeia HPLC

The use of biotherapeutic synthetic peptides in drug development has grown significantly in the past decade.Biotherapeutic synthetic peptides often have higher efficacy, safety, and tolerability in humans compared to small molecules. &nbsp;They also have lower production complexity and cost than protein-based biopharmaceuticals.While synthesis reactions are carefully controlled, impurities as well as degradation species are inevitably formed and must be characterized and identified. Having a robust Reversed-Phase (RP) method provides a solid foundation in the characterization and identification of these impurities. &nbsp;This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.<ul><li>Principles of Reversed-Phase LC and LC/MS of Peptides</li><li>Considerations in Selecting an Appropriate RP Column</li><li>Considerations in Selecting an Appropriate RP Eluent</li><li>Step by Step Approach to Developing a Robust Peptide Separations</li></ul><blockquote>Presenter: Bill Warren (Principal Consultant Product Manager, Waters Corporation)In 1986, Bill joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids.He currently is a Principal Consulting Bio separations Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.</blockquote>

Waters Corporation: Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.

LCMS webinars focusing on HPLC - page 23

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Live from the Lab: Solutions for biotherapeutics

Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

LC Chiral Theory and Method Development

Fundamentals of HPLC Separations for Small Molecule Analyses

Liquid Chromatography Fundamentals

Basics of Liquid Chromatography

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LCMS webinars focusing on HPLC - page 23

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results​

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Live from the Lab: Solutions for biotherapeutics

Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

LC Chiral Theory and Method Development

Fundamentals of HPLC Separations for Small Molecule Analyses

Liquid Chromatography Fundamentals

Basics of Liquid Chromatography

Related content

News from LabRulezLCMS Library - Week 27, 2026

Avoiding Common Syringe Filter Mistakes

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Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results