We are pleased to announce that a maintenance release is now available for KnowItAll 2025.Maintenance releases are an important way to introduce improvements and bug fixes between major releases to make sure users get the most out of KnowItAll solutions.Late 2024, we announced the KnowItAll 2025 major release featuring the new LC Expert tool, KnowItAll Data Control, KnowItAll Drug Classification Models, and various advances for spectral analysis workflows. This is the first update available after that release.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Know_It_All_LC_Expert_Streamline_component_identification_with_the_power_of_the_new_Know_It_All_LC_Expert_s_automated_workflow_84a090a7c2.jpg" alt="Wiley: NEW! KnowItAll LC Expert: Streamline component identification with the power of the new KnowItAll LC Expert’s automated workflow." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Know_It_All_LC_Expert_Streamline_component_identification_with_the_power_of_the_new_Know_It_All_LC_Expert_s_automated_workflow_84a090a7c2.jpg 245w," sizes="100vw" width="1735" height="960"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Know_It_All_drug_classification_models_25277cc5e0.jpg" alt="Wiley: NEW! KnowItAll drug classification models." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Know_It_All_drug_classification_models_25277cc5e0.jpg 245w," sizes="100vw" width="603" height="324">We encourage users to ensure they are using the most recent version of the software for best performance.<blockquote>Current users can <a target="_blank" rel="noopener noreferrer" href="https://get.knowitall.com/Download?_gl=1*18h0vjr*_gcl_au*Mjk3ODY0Mzg1LjE3MTMyNzcyMDEuMTkwNDkyNDI4NS4xNzEzMzY3NDc0LjE3MTMzNjc0Nzk.">download the most recent version here</a>.</blockquote><h4>Learn more about KnowItAll 2025:&nbsp;&nbsp;</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/whats-new-in-knowitall-2025/">What's New&nbsp;</a> &nbsp;&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-version-history/">Version History</a></li></ul><h4>About KnowItAll</h4>KnowItAll Software offers solutions to analyze and manage multiple types of spectral and chemical data in multiple file and instrument formats. KnowItAll's integrated toolsets eliminate the need for multiple software packages and increase overall lab efficiency and staff proficiency. Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis.<h4>About Wiley</h4>Wiley (NYSE: WLY) is one of the world’s largest publishers and a trusted leader in research and learning. Our industry-leading content, services, platforms, and knowledge networks are tailored to meet the evolving needs of our customers and partners, including researchers, students, instructors, professionals, institutions, and corporations. We empower knowledge-seekers to transform today’s biggest obstacles into tomorrow’s brightest opportunities. For more than two centuries, Wiley has been delivering on its timeless mission to unlock human potential. Visit us at <a target="_blank" rel="noopener noreferrer" href="https://www.wiley.com/en-ie">Wiley.com</a>.&nbsp;

KnowItAll 2025 Maintenance Release 2025.1 Available

SIELC has developed the first and only HPLC software that is accessible via the cloud - <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a>.<h4>&nbsp;Special Features:</h4><ul><li>Cloud Integration: Real-time data streaming and storage on HPLC.cloud ensure your experiment data is always backed up and accessible.</li><li>Remote Access: Control and monitor your system from anywhere — lab, office, or on the go — using a PC, tablet, or phone.</li><li>Data Security: All your results are stored in a centralized, secure location — so no data is ever lost.</li><li>Sync Capabilities: Chromatograms are synced with HPLC.cloud every second, providing real-time experiment updates.</li></ul><h4>Key Functionality:</h4><ul><li>Real-time Chromatogram Streaming: Watch live data as it’s being collected.</li><li>Sequence Building and Recording: Build, record, and store experiment sequences.</li><li>Method Storage: Save and reuse methods for consistency and efficiency.</li><li>Data Accessibility: Store all your sample and result data securely in the cloud, accessible anytime.</li><li>Remote Control: Start, stop, or modify analyses from any internet-connected device.</li><li>Comprehensive Data Analysis: Calibrate, analyze, and quantify directly within the cloud interface.</li><li>Mobile and PC Access: Use on PC, tablet, or smartphone for maximum flexibility.</li><li>Online Data Search: Easily search and access your data from anywhere.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg 225w," sizes="100vw" width="801" height="554">No software installation. No USBs. No data loss.

How to Use HPLC Cloud - A Complete Guide

News from LabRulezLCMS Library - Week 20, 2025

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<div><div>Customers across various industries, including pharma, biopharma, new modality companies, and academic institutions, seek to purify compounds such as medicinal chemistry, peptides, oligonucleotides, and impurities for drug discovery and development. Purification labs require high-capacity sample processing and fraction collection in smaller prep scales, along with more efficient and cost-effective purification solutions to accelerate drug-to-market timelines, reduce costs, and lower laboratory operating expenses.In this insightful webinar, discover how to elevate your analytical scale purification performance with the new Waters Analytical Scale Purification System, which offers higher efficiency, easier scale-up, and reduced operational costs with more predictable results.Paul Lefebvre, the General Manager at NeoPharm Boston, will be the guest speaker. With more than 25 years of experience in separations and purification, Paul's expertise spans various purification applications, including both small and large molecules. Paul will demonstrate three purification applications — GLP-1, oligos, and Spinetoram — using the Waters Analytical Scale Purification System. He will showcase how this new solution maximizes sample yield and purity, simplifies scale-up from method scouting, accelerates drug-to-market timelines, and reduces operating costs.Key takeaways:<ul><li>How to scale up a UPLC method to an analytical scale purification method for both small and large molecules</li><li>How to leverage the Analytical Scale Purification System with sub-5-μm particle size preparative columns to boost purification efficiency and reduce lab operation cost</li><li>Tips and tricks for GLP-1 peptides and oligonucleotide purification</li></ul><blockquote>Presenter: Darcy Shave (Principal Product Manager, Waters Corporation)Darcy Shave is currently a principal product manager in the Small Molecules group at Waters Corporation (Milford, MA, USA), responsible for the purification and hardware automation products. Darcy has been with Waters for over 20 years, starting as an applications chemist in the purification group. From there, he moved into a variety of business development roles before becoming a product manager for purification software about 10 years ago and more recently for the entire purification portfolio. He has a degree in Agricultural Chemistry from the University of Saskatchewan and worked in value added processing of natural products before joining Waters.Presenter:&nbsp;Michael R. Miller (Principal Scientist, Neopharm Boston)Michael has worked at Neopharm Boston for 4 years and has over 10 years of chromatographic and mass spectrometric experience. At SUNY University at Albany, he studied RNA Biochemistry and LC-MS/MS analysis of large RNAs. Joining Neopharm Boston in 2021 as an Analytical Scientist, Michael spent two years performing HPLC and SFC separations at analytical and preparative scale. Moving up to Lab Supervisor and ultimately Principal Scientist he using his large molecule knowledge to bring Neopharm Boston into the large molecule space.</blockquote></div></div>

Waters Corporation: Enhance Your Analytical Scale Purification Performance with Advanced Prep Solution

Enhance Your Analytical Scale Purification Performance with Advanced Prep Solution

In this webinar, discover how to elevate scale purification performance with new Waters Analytical Scale Purification System

Analytical Scale Purification with Advanced Prep Solution

Polysorbates and other surfactants are critical components of biopharmaceutical formulations as they prevent aggregation and the formation of proteinaceous particles, as well as to prevent surface interactions, which may cause protein degradation. &nbsp;However, polysorbates are prone to degradation through hydrolysis or oxidation and there is an increasing awareness and amount of research on this issue. &nbsp;Current methods to characterize surfactant degradation tend to be long, over 40 minutes, and have limited resolution, particularly the resolution between the free fatty acid and the polysorbate monoester. &nbsp;Here, we present a novel method and column designed for higher resolution and higher throughput analyses for polysorbate degradation.<blockquote>Presenter: Wendi Hale, PhD (Global Product Manager, Agilent Technologies, Inc.)Wendi is a global product manager for Agilent bio LC columns. She joined Agilent in 2017 as an LC/MS applications scientist, focused on biopharma applications. Her interest in biopharma applications started during her PhD at the University of Michigan under Prof. Kicki Håkansson, studying top-down and bottom-up protein analysis with FT-ICR mass spectrometry. She also studied phosphoproteomics during her post-doc at the University of Geneva in Switzerland.</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

Avoiding Polysorbate Pitfalls: A Novel Method to Characterize Polysorbate Degradation

In this webinar we present a novel method and column designed for higher resolution and higher throughput analyses for polysorbate degradation.

Novel Method to Characterize Polysorbate Degradation

<h4>Expand Your Expertise with Our Chromatography Webinar Series 2025</h4>A comprehensive journey through fundamental principles, HPLC optimization, and cutting-edge mixed-mode techniques. Led by industry experts, these free sessions provide practical insights and real-world applications to enhance your analytical skills.<h5>Session 3: Introduction to Mixed-Mode Chromatography in HPLC</h5><ul><li>What is mixed-mode chromatography?</li><li>Advantages over single-mode techniques (RP, IEX, HILIC, etc.)</li><li>Mechanisms of multiple interactions: hydrophobic, ionic, hydrogen bonding, and π-π interactions</li><li>How mixed-mode selectivity can improve complex separations</li></ul><blockquote>Presenter: Jovana Mitrovic (R&amp;D Chemist at SIELC Technologies)Jovana holds a Master of Science in Molecular Engineering from the Pritzker School of Molecular Engineering at the University of Chicago and a Bachelor of Science in Biomedical Sciences from St. John’s University. Her background in molecular and biomedical sciences supports a multidisciplinary approach to addressing complex analytical challenges.</blockquote>

SIELC Technologies: HPLC Educational Webinar Series

Introduction to Mixed-Mode Chromatography in HPLC

Join us for a webinar and learn about what is mixed-mode chromatography, advantages over single-mode techniques (RP, IEX, HILIC) and other useful things.

<h4>Expand Your Expertise with Our Chromatography Webinar Series 2025</h4>A comprehensive journey through fundamental principles, HPLC optimization, and cutting-edge mixed-mode techniques. Led by industry experts, these free sessions provide practical insights and real-world applications to enhance your analytical skills.<h5>Session 4: Practical Method Development in Mixed-Mode Chromatography</h5><ul><li>Selecting the right stationary and mobile phases</li><li>Optimization strategies: balancing retention, peak shape, and resolution</li><li>Overcoming common challenges in method development</li><li>Case studies: real-world applications in pharmaceuticals, biological samples, and complex matrices</li></ul><blockquote>Presenter: Jovana Mitrovic (R&amp;D Chemist at SIELC Technologies)Jovana holds a Master of Science in Molecular Engineering from the Pritzker School of Molecular Engineering at the University of Chicago and a Bachelor of Science in Biomedical Sciences from St. John’s University. Her background in molecular and biomedical sciences supports a multidisciplinary approach to addressing complex analytical challenges.</blockquote>

Practical Method Development in Mixed-Mode Chromatography

This webinar explains how to select the right stationary and mobile phases and how to overcome common challenges in method development.

<h4>Expand Your Expertise with Our Chromatography Webinar Series 2025</h4>A comprehensive journey through fundamental principles, HPLC optimization, and cutting-edge mixed-mode techniques. Led by industry experts, these free sessions provide practical insights and real-world applications to enhance your analytical skills.<h5>Session 5: Real-World Applications &amp; Future Innovations in Mixed-Mode HPLC</h5><ul><li>Advanced applications in pharmaceuticals, biotechnology, and food analysis</li><li>Strategies for handling complex sample matrices with mixed-mode chromatography</li><li>Emerging research and novel stationary phases</li><li>Future trends and developments in mixed-mode HPLC</li></ul><blockquote>Presenter: Jovana Mitrovic (R&amp;D Chemist at SIELC Technologies)Jovana holds a Master of Science in Molecular Engineering from the Pritzker School of Molecular Engineering at the University of Chicago and a Bachelor of Science in Biomedical Sciences from St. John’s University. Her background in molecular and biomedical sciences supports a multidisciplinary approach to addressing complex analytical challenges.</blockquote>

Real-World Applications & Future Innovations in Mixed-Mode HPLC

Join us for a webinar discussing advanced applications in pharmaceuticals, biotechnology, and food analysis 

Applications & Future Innovations in Mixed-Mode HPLC

Discover the Agilent InfinityLab LC Series Join us for an exclusive webinar introducing the future of liquid chromatography – the Agilent InfinityLab LC Series, designed to meet the challenges of tomorrow's laboratories today.&nbsp;During this session, you will:<ul><li>Get an in-depth look at the Agilent InfinityLab LC Series</li><li>Discover innovative features that enhance performance, usability, and sustainability</li><li>See how these systems can streamline your workflow and deliver reliable results</li><li>Engage with Agilent expert in a live Q&amp;A session</li></ul>Don’t miss this opportunity to stay ahead with cutting-edge HPLC technology!&nbsp;<blockquote>Presenter: Laura Montis</blockquote>

Altium Poland: Say Hello to the HPLC Made for Tomorrow

Say Hello to the HPLC Made for Tomorrow

We invite you to webinar series with Agilent Technologies, where we will present latest solutions in liquid chromatography

The recent rise in export rejections due to contamination from mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) has become a pressing concern for the food industry. Known for their links to health risks such as organ toxicity, carcinogenicity, and tissue accumulation, MOSH/ MOAH contamination underscores the urgent need to safeguard public health and comply with evolving regulations.Shimadzu, in collaboration with Axel Semrau, introduces an enhanced turnkey analytical solution to enable effective MOSH/MOAH analysis. Join our latest session in Digital Classrooms to gain exclusive insights into the new and improved MOSH/MOAH detection method, strategies for navigating regulatory compliance, and actionable solutions to mitigate contamination risks effectively.Tailored for regulators, private testing laboratories, and food manufacturers, this session is a must-attend to enhance your expertise to do food testing with compliance.Equip yourself with Shimadzu's solutions to address one of the food industry’s most significant challenges. Register now!<h5>Learning Objectives</h5><ul><li>Discover the latest trends and challenges surrounding MOSH/ MOAH contamination in the food industry.</li><li>Explore cutting-edge advancements in MOSH and MOAH analyzers, paving the way for more accurate and efficient detection.</li><li>Learn how Shimadzu’s innovative solutions can effectively manage contamination risks and ensure compliance with evolving regulations.</li></ul><blockquote>Presenter: Goh Wan Yu Elton (Market Specialist - Food And Environmental, Shimadzu (Asia Pacific) Pte Ltd)Elton dedicates his career to food analytics, ensuring the safety and quality of our daily consumption. As a Food Specialist at Shimadzu, he develops and applies advanced analytical techniques to detect and quantify compounds in food products, from contaminants to nutrients. Elton takes pride in advancing food science, enhancing safety, and building consumer trust. His goal is to make valuable contributions to analytical chemistry and the global food industry.</blockquote>

Shimadzu: Enhancing Food Testing: New And Improved Method For MOSH/MOAH Contamination

Enhancing Food Testing: New And Improved Method For MOSH/MOAH Contamination

Learn about MOSH/MOAH detection, regulatory tips, and solutions to reduce contamination risks in our latest Digital Classrooms session.

Food Testing: Method For MOSH/MOAH Contamination

<h4>Overview</h4>This webinar, co-hosted by Phenomenex and the Bio-Analytical Separation Science Group at Vrije Universiteit Brussel, will cover:<ul><li>The growing potential of oligonucleotide therapeutics and the importance of thorough impurity profiling.</li><li>Challenges in detecting critical impurities such as shortmers and phosphodiesters (PO) during synthesis.</li><li>Ion-pair reversed-phase liquid chromatography’s (IP-RPLC) mechanism of action for high-resolution impurity analysis.</li><li>Evaluating the impact of column preheaters on analyte dispersion and method performance.</li><li>The role of fluoroalcohols in enhancing LC separation and MS detection.</li><li>Comparing bio-inert columns for improved impurity profiling outcomes.</li></ul><h4>Key Learning Points</h4><ul><li>Understand the therapeutic mechanisms of oligonucleotides and the significance of base, ribose, and phosphate modifications.</li><li>Recognize the analytical challenges in oligonucleotide impurity profiling, including shortmers and phosphodiester impurities.</li><li>Learn how to develop and optimize IP-RPLC-MS methods for high-throughput analysis.</li><li>Evaluate the influence of preheater effects and fluoroalcohols on separation and detection sensitivity.</li><li>Compare the performance of bio-inert columns for impurity profiling.</li></ul><h4>Who Should Attend</h4><ul><li>Oligonucleotide Researchers: Ideal for scientists involved in developing and characterizing oligonucleotide therapeutics.</li><li>Analytical Chemists: Professionals focused on impurity profiling and method optimization using advanced chromatographic techniques.</li><li>Biopharmaceutical Quality Control Experts: Valuable for those ensuring the purity, stability, and safety of oligonucleotide-based therapeutics.</li><li>Contract Research Organizations</li><li>Biopharmaceutical Innovator Companies</li></ul><blockquote>Speaker: Dr. Sebastiaan Eeltink &nbsp;(Professor and lead investigator of the Eeltink Laboratory at the University of Brussel &amp; Editor of Analytical Science Advances at Wiley)Dr. Sebastiaan Eeltink is a Full Professor and lead investigator of the Eeltink Laboratory at the University of Brussel and an Editor of Analytical Science Advances at Wiley. His team focuses on developing and designing novel analytics for the high-resolution profiling of biomolecules and biomolecular interactions using ultrahigh-pressure liquid chromatography (UHPLC) and multi-dimensional liquid chromatographic workflows with mass spectrometry (MS) detection. In addition, his team also manages the University’s proteomics platform which offers high-performance LC-MS proteomics profiling expertise and advise to academic and industrial collaborators.Speaker: Quang-Dong Bui &nbsp;(PhD student at University of Brussels)Quang-Dong Bui is currently a PhD student at University of Brussels under the supervision of Professor Sebastiaan Eeltink, where he develops analytical methods for key oligonucleotide projects and is an active scientist in the oligonucleotide and mRNA research community.</blockquote>

Phenomenex: Advanced IP-RPLC-MS Strategies for High-Resolution Oligonucleotide Impurity Profiling

Advanced IP-RPLC-MS Strategies for High-Resolution Oligonucleotide Impurity Profiling

Discover advanced IP-RPLC-MS methods for high-resolution oligonucleotide impurity analysis. Enhance your analytical workflows and data precision.​

Advanced IP-RPLC-MS for Oligonucleotide Profiling

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Capillary scale liquid chromatography (LC) offers separations comparable to the more traditional analytical scale at a fraction of the solvent consumption. In this webinar, learn how your lab can benefit from a compact capillary scale LC instrument that can be hand-carried anywhere and used on-the-spot.In order to be considered as a viable replacement for larger scale systems, a system must demonstrate its robustness and repeatability. Key repeatability metrics will be discussed, including interday and intraday repeatability, and injection carryover. Discover how these metrics provide valuable insights to those looking to integrate capillary scale separations into their workflows.<h5>Key learning objectives</h5><ul><li>See how capillary scale chromatography can help your lab save solvent and sample</li><li>Learn about compact capillary scale instrumentation</li><li>View methods for the quantification of system repeatability and discover how the Axcend Focus LC® performs</li></ul><h5>Who should attend?</h5><ul><li>Chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners, and academics.</li></ul><h5>Certificate of attendance</h5><ul><li>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</li><li>If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</li></ul><blockquote>Presenter: Dr. Samuel Foster (Application Scientist, Axcend)Dr. Samuel Foster completed his Ph.D. in Pharmaceutical Chemistry from Rowan University in 2025. His research has focused on the development and application of capillary scale liquid chromatography instrumentation. He currently works at Axcend as an application scientist focusing on the development of chromatographic workflows for a variety of analyte classes including oligonucleotides, monoclonal antibodies, and drugs of abuse.Moderator: Sarah Thomas (Associate Editor, SelectScience)</blockquote></div></div></div>

SelectScience: Save solvent and samples with compact capillary scale LC

Save solvent and samples with compact capillary scale LC

Join this educational webinar where Senior Product Manager (Thermo Fisher Scientific) will introduce NanoDrop™ Ultra for nucleic acid quantification

Save Solvent and Sample with Capillary Scale LC

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>When looking for new ways to increase sample throughput in proteomics, without compromising on proteome profiling or quantitation, Thermo Scientific Vanquish Neo UHPLC systems and Thermo Scientific µPAC Neo columns can really make a difference.Using the Vanquish Neo system, with its newly introduced tandem workflow, a dual separation workflow in a single system is created to maximize MS utilization for high-throughput and deep-dive proteomics.Benefiting from the perfectly ordered microfabrication of the Thermo Scientific™ µPAC™ Neo columns, this workflow has advanced proteomics research into the next era with maximal reproducibility and robustness.Join this free webinar to learn more about how you can employ Vanquish Neo Tandem Workflow systems and µPAC Neo columns in your lab.In this webinar, we present to you how to meet increasing sample queue demands without compromising separation and MS results by:<ul><li>using the Vanquish Neo UHPLC system for low-flow separations in proteomics</li><li>maximizing mass spectrometer utilization with the Vanquish Neo Tandem Direct-Injection workflow for increased sample throughput, and minimized column carryover</li><li>Tandem Direct-Injection workflow for nano, capillary and micro-flow applications</li><li>utilizing the unique micro-pillar array backbone to provide both, highest robustness and consistency</li></ul>Cannot join on May 20? Please register anyway to automatically receive the link to the recording.<blockquote>Presenter: Robert van Ling (Product Manager Low-flow HPLC Columns, Thermo Fisher Scientific)Experienced marketeer &amp; business developer, as well as technical sales support specialist, for the biopharma and omics community. 25+ years of protein, peptide and RNA chromatography and LC/MS experience, focused on providing researchers with innovative and high-performance workflows and solutions.</blockquote></div></div></div>

Thermo Fisher Scientific: Innovative separation approaches for MS-based proteomics

Innovative separation approaches for MS-based proteomics

Join this free webinar to learn more about how you can employ Vanquish Neo Tandem Workflow systems and µPAC Neo columns in your lab.

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