Solid Phase Extraction (SPE) is one of the most highly selective sample preparation methods, meaning that the targeted analytes and their matrices must be considered when choosing the correct SPE sorbent and format. Drying down the sorbent before elution is an important step.<ul><li>Wash solvents are used to flush out unwanted compounds (i.e., contaminants). If the wash solvent is not completely removed, then residual wash solvent could contaminate the final elution.</li><li>If you do not dry the SPE sufficiently, it could make the final solution less concentrated and result in a reduction in sensitivity.</li><li>Drying helps physically break down the interactions between the sorbent and analyte for a more concentrated extract.</li></ul>After the wash solvent has been passed through the cartridge, continue to apply negative pressure (vacuum) or positive pressure. Use this pressure to dry the cartridge for 2 to 5 minutes (or until dry). Turn off the pressure and wipe the tips of the manifold needles to remove any residual solvent.<h5>Notes</h5><ol><li>Reproducibility: Drying removes traces of residual sample/matrix and wash solvent from the sorbent. This produces a more concentrated final extract with a consistent volume and composition and yields better reproducibility and higher recoveries.</li><li>Immiscibility: Drying is especially critical when using elution solvents that are immiscible with water (e.g. hexane, ethyl acetate, and methylene chloride). Residual water in the sorbent bed may prevent dissolution of the analyte in the elution solvent, resulting in low recoveries and irreproducibility.</li><li>Over-drying: Avoid over-drying the sorbent, since in some cases, analyte recovery may decrease with excessive vacuum or drying times above 5 minutes. Dry cartridges look distinctly different from moist ones. Make a note of the cartridge appearance before and after conditioning. Also, you could touch the cartridge while it is drying; wet cartridges feel colder than dry cartridges.&nbsp;</li></ol>

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

<h3>Mastering Ion-Pair Reversed-Phase Chromatography for Oligonucleotide Analysis</h3><h4>Why Purity Is Critical in the Oligonucleotide Era</h4>Oligonucleotides — short, synthetic strands of DNA or RNA — are central to modern biotechnology. They power gene therapies, advanced diagnostics, and cutting-edge molecular research. However, before these molecules can be used in clinical or research settings, their purity and structural integrity must be thoroughly verified. High-resolution chromatographic analysis plays a crucial role in ensuring quality and safety.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Separation_Example_of_Oligonucleotides_5577514561.jpg" alt="KNAUER: Figure 1 - Separation Example of Oligonucleotides." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Separation_Example_of_Oligonucleotides_5577514561.jpg 245w," sizes="100vw" width="1024" height="368"><h4>Understanding Ion-Pair Reversed-Phase (IP-RP) Chromatography</h4>For scientists involved in oligonucleotide synthesis and characterization, Ion-Pair Reversed-Phase Chromatography (IP-RP) is one of the most effective analytical tools available.Unlike separation techniques that rely solely on charge differences, IP-RP combines:<ul><li>Ionic interactions</li><li>Hydrophobic interactions</li></ul>This dual retention mechanism enables exceptional resolution — even for oligonucleotides that differ by only a single nucleotide or subtle chemical modification. Whether assessing synthesis efficiency or purifying therapeutic-grade material, IP-RP provides reliable, reproducible separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overview_of_IP_RP_Mode_prinicple_ecd54b6e9c.jpg" alt="KNAUER: Figure 2 - Overview of IP-RP Mode prinicple." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overview_of_IP_RP_Mode_prinicple_ecd54b6e9c.jpg 245w," sizes="100vw" width="1024" height="456"><h4>The Principle Behind Ion-Pairing</h4>Oligonucleotides possess a highly negatively charged phosphate backbone. In a traditional reversed-phase system, such hydrophilic, charged molecules would show little retention.IP-RP overcomes this limitation by introducing a positively charged ion-pairing reagent into the mobile phase. The cation associates with the negatively charged oligonucleotide, forming a transient ion pair with increased hydrophobic character. This modified complex can now interact with the hydrophobic stationary phase.Retention strength depends on:<ul><li>The hydrophobicity of the ion-pair reagent</li><li>The strength of ionic interaction</li><li>The applied organic gradient</li></ul>During gradient elution (commonly using acetonitrile), analytes are released in a controlled manner according to size, sequence, and chemical modifications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Ion_Pair_Reversed_Phase_Principle_9a4fcd12f2.jpg" alt="KNAUER: Figure 3 - Ion-Pair Reversed Phase Principle." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Ion_Pair_Reversed_Phase_Principle_9a4fcd12f2.jpg 245w," sizes="100vw" width="1024" height="393"><h4>Choosing the Right Ion-Pair Reagent</h4>Successful IP-RP separation depends heavily on selecting an appropriate ion-pair reagent. Two key factors determine retention behavior:<ol><li>Alkyl chain length</li><li>Charge density</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Ion_Pair_Classification_eaad172a4e.jpg" alt="KNAUER: Figure 4 - Ion-Pair Classification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Ion_Pair_Classification_eaad172a4e.jpg 245w," sizes="100vw" width="1024" height="398"><h4>Strong Cationic Ion-Pair Reagents</h4>Ion-pair reagents with longer alkyl chains provide stronger hydrophobic interactions and increased retention. A widely used example is tetrabutylammonium (TBA⁺), frequently applied for strongly retained anionic analytes.These reagents are particularly useful when high retention strength is required.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Strong_Cationic_Ion_Pair_Reagents_8dbf00c57e.jpg" alt="KNAUER: Table 1 - Strong Cationic Ion-Pair Reagents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Strong_Cationic_Ion_Pair_Reagents_8dbf00c57e.jpg 245w," sizes="100vw" width="1024" height="396"><h4>Volatile (Weaker) Ion-Pair Reagents</h4>Short-chain ion-pair reagents generate weaker retention but offer a significant advantage: compatibility with LC–MS detection.Triethylammonium (TEA⁺) is among the most commonly used reagents for oligonucleotide analysis, particularly when mass spectrometric detection is involved. Other options, such as diisopropylethylammonium (DIEA⁺), are also widely applied, often combined with HFIP to enhance MS performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_2_Weak_Cationic_Ion_Pair_Reagents_6332a4975e.jpg" alt="KNAUER: Table 2 - Weak Cationic Ion-Pair Reagents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_2_Weak_Cationic_Ion_Pair_Reagents_6332a4975e.jpg 245w," sizes="100vw" width="1024" height="283"><h4>Building an IP-RP Method for Oligonucleotides</h4>Developing a robust IP-RP method follows a logical sequence:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_3_IP_RP_Method_Step_Procedure_for_Oligos_c2216c24a0.jpg" alt="KNAUER: Table 3 - IP-RP Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_3_IP_RP_Method_Step_Procedure_for_Oligos_c2216c24a0.jpg 245w," sizes="100vw" width="1024" height="304"><h5>1. Characterize the Analyte</h5>Oligonucleotides typically contain 10–50+ nucleotides and exhibit high negative charge density. This necessitates a suitable cationic partner such as TEA⁺ or DIEA⁺.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_Cationic_Ion_Pair_Principle_for_Oligonucleotides_cb2dfb7457.jpg" alt="KNAUER: Figure 5 - Cationic Ion-Pair Principle for Oligonucleotides." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_Cationic_Ion_Pair_Principle_for_Oligonucleotides_cb2dfb7457.jpg 245w," sizes="100vw" width="512" height="320"><h5>2. Select the Column</h5>Use a reversed-phase column with:<ul><li>Large pore size (≈300 Å)</li><li>Appropriate internal diameter</li></ul>These parameters support the so-called “ON–OFF” retention mechanism — a bind-and-elute behavior where analytes are strongly retained until the gradient triggers rapid elution.<h5>3. Optimize the Mobile Phase</h5>A typical starting condition includes:<ul><li>10 mM TEA buffer at pH ~7</li><li>Acetonitrile as organic modifier</li></ul>Gradient programs from approximately 5% to 50% organic solvent over 15–30 minutes are commonly effective for complex oligonucleotide mixtures.<h5>4. Adjust Retention Strength if Needed</h5>To increase retention:<ul><li>Raise ion-pair concentration (up to ~100 mM if required)</li><li>Select a more hydrophobic ion-pair reagent</li></ul><h5>5. Validate the Method</h5>Ensure robustness, sensitivity, and reproducibility by carefully evaluating:<ul><li>UV chromatograms</li><li>MS traces</li><li>Peak shape and resolution</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_UV_MS_traces_of_IP_RP_Oligonucleotide_Analysis_81faf4e9f0.jpg" alt="KNAUER: Figure 6 - UV-MS traces of IP-RP Oligonucleotide Analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_UV_MS_traces_of_IP_RP_Oligonucleotide_Analysis_81faf4e9f0.jpg 245w," sizes="100vw" width="1024" height="365"><h4>Why IP-RP Is Considered the Gold Standard</h4>Ion-Pair Reversed-Phase Chromatography uniquely combines the resolving power of reversed-phase LC with the ability to retain highly polar, charged biomolecules.Its advantages include:<ul><li>Tunable selectivity</li><li>Compatibility with UV and MS detection</li><li>Scalability from analytical to preparative workflows</li><li>Applicability from research to pharmaceutical production</li></ul>By transforming highly charged oligonucleotides into hydrophobic ion-pair complexes, IP-RP enables precise and reproducible separations that would otherwise be impossible in conventional reversed-phase systems.For these reasons, IP-RP is widely regarded as the benchmark technique for oligonucleotide analysis.<h4>Final Thoughts</h4>Mastering IP-RP is less intimidating than it may seem. A successful method comes down to:<ul><li>Selecting the right ion-pair reagent</li><li>Choosing a suitable column</li><li>Optimizing gradient conditions</li><li>Fine-tuning temperature and concentration parameters</li></ul>With thoughtful method development, IP-RP delivers sharp peaks, clean separations, and reliable performance — fully compatible with modern mass spectrometry workflows and ready to meet the demands of advanced oligonucleotide analysis.

Oligos Made Easy - Part 3: IP-RP

News from LabRulezLCMS Library - Week 08, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<div>Join us for the last 5 series on building the perfect method for light scattering analysis.Learn advanced tools and techniques to master ASTRA software, from normalization to DLS with flowing systems.<h4>Session Overview:</h4><ul><li>Part 6 of 10: Building the Perfect Method - Normalization, Alignment, &amp; Inter-Detector Band Broadening Corrections<ul><li>Friday, September 26</li></ul></li><li>Part 7 of 10: Navigating Sample Parameters – A Close Look at dn/dc and Extinction Coefficients<ul><li>Friday, October 3</li></ul></li><li>Part 8 of 10: Advanced Characterization Tools for Multi-Component Analytes - Protein Conjugates, AAV, LNP and ADC<ul><li>Friday, October 10</li></ul></li><li><h5>Part 9 of 10: ASTRA for Particles: When and How to Deploy</h5><ul><li>Friday, October 17</li></ul></li><li>Part 10 of 10: DLS with Flowing Systems – Leveraging Rh and the Burchard-Stockmayer Plot in ASTRA<ul><li>Friday, October 24</li></ul></li></ul><h4>Why Attend:</h4><ul><li>Deepen your understanding of light scattering and ASTRA.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Andy Meyer.</li></ul>The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.<blockquote>Hosted by: Dr. Andy MeyerDr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt's "Light Scattering University" and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Principal Trainer for Waters | Wyatt Technology. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.</blockquote></div>

Waters Corporation: Becoming an ASTRA Expert

Becoming an ASTRA Expert: Part 9

Find out how and when to use ASTRA for particle characterization. Learn tips for Rh, radius distributions and aggregation analysis.

Trick-or-Treat, want to listen to something sweet? Join our speakers to discuss all things sugar at this glycan imaging seminar!<blockquote>Presenter: Gus Grey (Associate Professor, Department of Physiology, University of Auckland, New Zealand)Presenter: Caroline Kittrell (PhD Candidate, Drake Lab Pharmacology and Immunology, Medical University of South Carolina, USA)Moderator: Dr. Jessica MooreModerator: Kelsey Buondono</blockquote>

US HUPO: Sugar: The sweetest molecule in imaging

Sugar: The sweetest molecule in imaging

Trick-or-Treat, want to listen to something sweet? Join our speakers to discuss all things sugar at this glycan imaging seminar!

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>For decades, analyzing proteins has required laborious and time-consuming sodium dodecyl sulfate (SDS) polyacrylamide gels resulting in inconsistent results and limited quantification. Advances in capillary-based separations now offer a faster, more reproducible way to assess protein size, purity and quantity with minimal hands-on time. In this webinar, our expert speakers will highlight the limitations of traditional gels, explore an advanced &nbsp;streamlined approach and demonstrate how capillary electrophoresis technology can improve data quality and free up valuable lab resources. Real-time interaction, through a live Q&amp;A session, will help you translate these insights into action within your own workflow.<h5>Attend this webinar to:</h5><ul><li>Understand modern advancements and how they compare to long-standing techniques</li><li>Learn how an automated separation method can overcome common SDS-gel challenges to improve speed, reproducibility and quantification</li><li>Gain knowledge, with a live demonstration of Agilent’s ProteoAnalyzer system, on how you can modernize your protein analysis strategy</li></ul>For Research Use Only. Not for use in diagnostic procedures.&nbsp;<blockquote>Presenter: Kyle Luttgeharm, PhD (Product Manager, Agilent Technologies)Presenter:&nbsp;Samina Kaufmann (Product Specialist for Sample QC, Agilent Technologies)</blockquote></div></div></div>

Agilent Technologies: A Modern Approach for Reliable Protein Denaturing Electrophoresis

A Modern Approach for Reliable Protein Denaturing Electrophoresis

See how Agilent ProteoAnalyzer improves protein analysis with capillary electrophoresis—faster, reproducible results vs SDS gels, plus a live demo and Q&A.

Approach for Reliable Protein Denaturing Electrophoresis

<div>Biotherapeutics such as monoclonal antibodies, fusion proteins, peptides and cytokines are increasingly complex, creating challenges for rapid and accurate quality characterization. Traditional liquid chromatography methods, such as size exclusion chromatography and hydrophobic interaction chromatography, often struggle with electrospray ionization mass spectrometry (MS) compatibility, slowing impurity identification.This webinar introduces a refined matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS workflow that streamlines analysis, enables high-throughput screening and integrates with existing development pipelines. Attendees will gain insights into approaches that address structural complexity, enhance analytical efficiency and support faster decision-making in biotherapeutic development.Join this webinar for:<ul><li>Learn about the development of cutting-edge biotherapeutic characterization methods using MALDI-MS</li><li>Discover an automated impurity identification workflow, suitable for different modalities and based on MALDI</li><li>Explore methodologies which analyze therapeutic polyethylene glycol linked proteins</li><li>Assess the development of cell-based membrane permeability assay using MALDI-MS</li></ul><blockquote>Presenter: Gregory F. Pirrone, PhD (Associate Principal Scientist, Merck &amp; Co., Inc.)Dr. Gregory F. Pirrone is an associate principal scientist in analytical research and development at Merck research laboratories (MRL). He earned his PhD from Northeastern University under the guidance of Dr. John R. Engen.Presenter: Alexey A. Makarov, PhD (Senior Principal Scientist, Merck &amp; Co., Inc.)Dr. Alexey A. Makarov is a senior principal scientist in the analytical research and development (ARD) department at Merck research laboratories (MRL). He earned his PhD in chemistry from Stevens Institute of Technology, New Jersey and has over 20 years of experience in the pharmaceutical industry.Presenter: Elizabeth F. Bayne, PhD (Senior Scientist, Merck &amp; Co., Inc.)Dr. Elizabeth F. Bayne is a senior scientist in analytical research and development at Merck Research Laboratories. Elizabeth earned her PhD in chemistry from the University of Wisconsin - Madison in 2023 under Professor Ying Ge, specializing in mass spectrometry-based intact protein analysis, multi-omics integration of proteomics and metabolomics data to better understand cardiac systems.</blockquote></div>

Technology Networks: Enhancing Biotherapeutic Characterization With MALDI Mass Spectrometry

Enhancing Biotherapeutic Characterization With MALDI Mass Spectrometry

Join this webinar to explore MALDI-TOF MS workflows for biotherapeutics, enabling high-throughput impurity ID and robust analysis of complex modalities.

Biotherapeutic Characterization With MALDI MS

Join our upcoming webinar to explore the innovative approach of Multi-Attribute Monitoring (MAM) for mRNA medicines using online partial RNase T1 digest and LC-MS. Mark Dickman from the University of Sheffield showcases a novel workflow to enhance the precision and efficiency of mRNA analysis.Learn about the methodology, applications and benefits of mRNA attribute monitoring with LC-MS and the use of novel software additions with Thermo Scientific™ BioPharma Finder™ software.Don't miss this opportunity to stay ahead in the rapidly evolving field of mRNA therapeutics.<h5>Key Learning Objectives:</h5><ul><li>Understand the principles and methodology of Multi-Attribute Monitoring (MAM) for mRNA medicines using online partial RNase T1 digest and LC-MS.</li><li>Learn how to apply RNase T1 digest and LC-MS techniques to enhance precision and efficiency in mRNA medicine analysis.</li><li>Gain insights into the benefits of MAM for ensuring better quality control and regulatory compliance in the development and production of mRNA therapeutics.</li></ul><h5>Who Should Attend:</h5><ul><li>Laboratory managers</li><li>Chromatography and mass spectrometry scientists</li><li>mRNA researchers</li><li>Biologics production scientists</li><li>QC analysts</li></ul><blockquote>Presenter: Mark J. Dickman (Professor, University of Sheffield)Presenter:&nbsp;Alexandra Taylor (Contributing Editor, C&amp;EN Media Group)</blockquote>

C&EN: Multi-Attribute Monitoring of mRNA Medicines Using Online Partial RNase T1 digest and LC-MS

Multi-Attribute Monitoring of mRNA Medicines Using Online Partial RNase T1 digest and LC-MS

Join webinar to explore innovative approach of Multi-Attribute Monitoring (MAM) for mRNA medicines using online partial RNase T1 digest and LC-MS.

Multi-Attribute Monitoring of mRNA by LC-MS & RNase T1

The Analytical Scientist is hosting a live roundtable discussion on sample preparation on October 21 at 3:00–4:00 PM (CEST) / 9:00–10:00 AM (EDT).Sample preparation has seen remarkable progress over the past few decades – from powerful new technologies and deeper fundamental insights to a wealth of breakthrough applications. Yet despite this momentum, sample prep is still too often seen as an “art” rather than a rigorous science, or as merely application-driven.This interactive session will challenge that perception by bringing together leading voices in the field to discuss how cutting-edge innovations are driving efficiency, precision, and reproducibility – while also addressing the growing imperative for greener, more sustainable laboratory practices.Join us for a dynamic roundtable conversation exploring the latest advances, best practices, and forward-looking strategies shaping the future of sample preparation – and take the opportunity to put your questions directly to the experts.<blockquote>Presenter: Marcela Segundo (Professor, Member of Executive Board, Faculty of Pharmacy, University of Porto)Presenter: Marcello Locatelli (Associate Professor of Analytical Chemistry, Department of Science, University "G. d' Annunzio" Chieti-Pescara, Italy)Presenter: Stig Pedersen-Bjergaard (Professor, University of Oslo, Norway)</blockquote>

The Analytical Scientist: State-of-the-Art Sample Preparation – Innovation to Sustainability

State-of-the-Art Sample Preparation – Innovation to Sustainability

Join our live roundtable discussion on pioneering new tools, greener workflows, and a bold vision for the future of sample preparation

State-of-the-Art Sample Preparation

<div>This technical webinar provides a comprehensive overview of high-performance liquid chromatography (HPLC) detection technologies commonly utilized in bioseparations. It will detail the operational principles, analytical capabilities, and performance characteristics of UV detection systems, including tunable UV (TUV) and photodiode array (PDA) detectors.The session will further explore complementary detection modalities such as fluorescence (FLR), evaporative light scattering (ELS), and single quadrupole (SQ) mass detection. Each detection technique will be examined, highlighting its working mechanism, strengths, limitations, and suitability for specific analytical tasks.Application examples will demonstrate how each detector supports specific analytical workflows, enabling informed decisions for method development and routine analysis.<blockquote>Presenter: Jennifer Simeone (Principal Consulting Scientist, Waters Corporation)Jennifer Simeone serves as Principal Consulting Scientist in the LC-MS Business Strategy Group at Waters Corporation in Milford, MA. With nearly 20 years of experience, she brings deep expertise in both chromatography and LC instrumentation, specializing in how design innovations shape method performance and enable seamless method transfer across LC platforms. Her role blends scientific rigor with strategic insight, driving product development and advancing analytical capabilities.Presenter: Kieron Faherty, Ph.D. (Principal Separations and Mass Spectrometry Market Development Manager, Waters Corporation)Kieron Faherty holds a B.Sc. (Hons) in Chemistry from the University of Reading in the UK and a Ph.D. in Chemistry from the University of Massachusetts, Amherst. With over two decades of dedicated service at Waters Corporation, Kieron has built a career focused on customer success and technological excellence.Starting as a field service engineer, Kieron gained in-depth experience with quadrupole and time-of-flight MS systems. He spent fourteen years in the Field Applications Group, ultimately leading a team that specialized in large molecule applications. In his current role as the Principal Market Development Manager for Separations and Mass Spectrometry, Kieron continues to leverage his extensive knowledge and commitment to cutting-edge technology to support and enhance customer success.</blockquote></div>

Waters Corporation: Advancing Bioseparations: A Deep Dive into HPLC Detection Technologies

Advancing Bioseparations: A Deep Dive into HPLC Detection Technologies

This technical webinar provides a comprehensive overview of HPLC detection technologies commonly utilized in bioseparations. 

Advancing Bioseparations: Deep Dive into HPLC Detection

Transferring analytical methods between laboratories is essential in regulated industries, but often deceptively complex. Although reproducibility should be straightforward in theory, inconsistencies in execution across labs can result in unreliable data, potentially triggering false positives or negatives. These missteps pose serious risks: from product recalls and delayed approvals to regulatory citations and costly batch rejections.<h5>In this webinar, we'll discuss:</h5><ul><li>Comparative testing as a proven strategy for assessing method transfer success, examine its strengths and weaknesses</li><li>The importance of continuous monitoring for long-term robustness and data integrity</li><li>Innovative digital tools that can simplify and accelerate both method transfer and post-transfer monitoring</li></ul>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.<blockquote>Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.Presenter:&nbsp;Zoe Chung (Principal Product Marketing Manager, Waters Corporation)Zoe Chung is a Product Marketing Manager at Waters, where she specializes in Empower Software Solutions. With over eight years of experience in marketing—and the last four focused on cloud-native technologies in the life sciences sector—Zoe brings a deep understanding of how digital innovation can transform scientific workflows. Her passion lies in advancing the next generation of software tools that enhance scientific productivity, unlock new ways to utilize data, and accelerate scientific discovery. At Waters, Zoe plays a key role in communicating the evolving capabilities of the Empower portfolio. She helps customers understand how to leverage cloud applications to gain faster, deeper insights from their chromatography data. Zoe is a dynamic advocate for modernizing lab informatics and is dedicated to helping organizations harness the full potential of their data through smarter, more connected solutions.</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Data-Driven Approaches to Minimize Risk and Promote Data Quality

Empower Tips: Ask Me Anything About Data-Driven Approaches to Minimize Risk and Promote Data Quality

Streamline method transfer with comparative testing, monitoring and Empower software—cut errors and protect data integrity. Live Q&A with Waters experts.

Empower Tips Data-Driven Approaches to Minimize Risk

<div>The accurate, precise and reproducible measurement of amino acids is critical in the detection and monitoring of inherited metabolic disorders such as PKU, MSUD, UCDs etc. Waters' Kairos Amino Acid offers mass spectrometry based detection that is accurate, precise and enables the quantification of 40+ amino acids.&nbsp;In this webinar, Marijn Van Hulle from Waters will provide an overview of the existing Kairos Amino Acid kit as well as a preview of upcoming optimziations to the kit's method. Additionally, Kiara Theron from McMaster Children's Hospital will present the outcome of her validation studies of the Kairos Amino Acid Kit in plasma, urine &amp; CSF patient samples.<h4>Learning points:</h4><ul><li>Description of the kit &amp; overview of the AccQTag derivatization chemistry</li><li>Advantages of the status quo Kairos Amino Acid Kit&nbsp;</li><li>Overview of the optimization changes for the Kairos Amino Acid Kit</li><li>Validation of the Kairos Amino Acid kit in plasma at McMaster Children's Hospital</li></ul><h4>Who should attend:</h4><ul><li>Lab Scientists/Managers/Directors in General Hospitals or Children Hospitals and Reference Labs interested in developing and validating an LDT for Amino Acid analysis for routine clinical care&nbsp;</li></ul><blockquote>Presenter: Kiara Theron (Research Assistant &amp; Master's student at McMaster University Pathology &amp; Molecular Medicine, McMaster Children's Hospital)Kiara Theron is a second year Master’s student in McMaster’s Medical Sciences graduate program. She is currently working under Dr. Murray Potter at McMaster Children’s Hospital to optimize and validate LC-MS/MS methods for biochemical monitoring in patients with inherited metabolic disorders. Her more recent work has focussed on amino acid analysis for these patients as part of which she optimized and validated the Kairos Amino Acid kit for plasma, and urine, and CSF samples against the lab's predecessor method which was based on the MassTrak Amino Acid Kit.Presenter: Marijn Van Hulle (Global Director for Clinical Applications &amp; Support, Water Corporation)Marijn Van Hulle is the Global Director for Clinical Applications &amp; Support. He oversees field applications, global escalations of chemistry, IVD kit and consumables support, method transfers, and the application notes pipeline in collaboration with R&amp;D. Marijn joined Waters in 2004 as an LC-MS application specialist in the Benelux after earning his PhD in analytical chemistry from Ghent University. He has (co)authored nine Waters application notes and four peer-reviewed papers.</blockquote></div>

Waters Corporation: Optimization and Validation of the Kairos Amino Acid Kit in a hospital laboratory

Optimization and Validation of the Kairos Amino Acid Kit in a hospital laboratory

In this webinar, Waters will provide overview of the existing Kairos Amino Acid kit as well as a preview of upcoming optimziations to the kit's method.

Kairos Amino Acid Kit in a hospital laboratory

Time is a critical resource in laboratory operations. However, challenges such as redundant sample data management, manual data handling and transferring of results as well as time-consuming approval processes are common in many labs.In our upcoming webinar on October 21, we will explore how digitalizing laboratory workflows address these issues head-on. Based on a best-practice example we will demonstrate how the ready-to-use interfaces of the 1LIMS Laboratory Information Management System, OMNIS Software, and some lab devices facilitate process optimization through seamless connectivity and integration. As we will demonstrate with our example workflow, time savings of more than one minute per analysis are feasible.In a Q&amp;A session at the end of this webinar, we will be happy to answer any questions you can have about digitalizing laboratory workflows. Don’t miss this opportunity and learn more about how to make your routine analysis more efficient.<h4>Key takeaways of this webinar:</h4><ul><li>Understand why connectivity is key to integrating your laboratory operations</li><li>Understand how seamless integration enables better planning and resource management</li><li>How to maximize efficiency of your laboratory operations</li><li>How to transform your laboratory into a fully integrated, digital ecosystem&nbsp;</li></ul><h4>Who should attend?</h4><ul><li>Laboratory managers, IT managers, and anyone interested in making their laboratory processes more efficient.</li></ul><blockquote>Presenter: Simon Böhm, PhD (Head of Comptence Center Software, Metrohm International Headquarters)Simon Böhm is Head of Competence Center Software at Metrohm International Headquarters in Herisau, Switzerland. He joined Metrohm Germany in 2017 as Product Specialist for titration. After being involved in rolling out OMNIS at customer sites in Germany, he took the opportunity to become global Product Manager at Metrohm International Headquarters and bring in his extensive experience. Simon studied chemistry at the University of Constance and earned his PhD from the ETH Zurich in physical chemistry.Presenter: Philipp Osterwalder (Co-founder and CEO, 1LIMS Lab and Quality Solution)Philipp Osterwalder is a skilled laboratory technician with further expertise as a biomedical analyst. While studying to become an environmental engineer specialising in eco-technology and ecological analytics, he founded 1LIMS as co-founder and current CEO to digitalize laboratories with a simple, flexible, smart solution. Today, 1LIMS is an international competence centre in lab process optimisation, laboratory digitalisation and automation, focusing on holistic expertise using lean Six Sigma.</blockquote>

Metrohm: Time is money - how digitalized workflows enhance laboratory efficiency

Time is money - how digitalized workflows enhance laboratory efficiency

Discover how OMNIS and 1LIMS integration boosts lab efficiency, saves time, and enables full digital connectivity.

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