<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Thermo Fisher Scientific

Shimadzu Corporation

SCIEX

Waters Corporation

<h4><strong>LC-MS/MS fundamentals webinar series 2.0</strong></h4>
<p><strong>Advanced techniques for data acquisition and processing</strong></p>
<p><strong>Elevate your analytical expertise with advanced techniques and workflows</strong></p>
<p>Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.</p>
<p>Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.</p>
<h4><strong>This webinar:</strong></h4>
<p>July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <strong>Excel strategies for mass spectrometry data analysis</strong></p>
<p>Take an in-depth look at when and how to use Excel to increase your productivity.</p>
<h4><strong>Other webinars from the series:</strong></h4>
<p>May 7, 2024 11:00 a.m. - 11:30 a.m. CET    - <a href="https://lcms.cz/webinar/3820"><strong>Beyond the fundamentals</strong></a></p>
<p>May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3852"><strong>Advanced MS/MS method building</strong></a></p>
<p>Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.</p>
<p>June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853"><strong>Mastering chromatography</strong></a></p>
<p>Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.</p>
<p>June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854"><strong>It&#39;s a TRAP! Getting the most out of your QTRAP system</strong></a></p>
<p>Learn when to utilize QTRAP functionality to create more selective and sensitive methods.</p>
<p>July 2, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855"><strong>Troubleshooting at the source</strong></a></p>
<p>Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.</p>
<p>July 16, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3856"><strong>Automating data processing</strong></a></p>
<p>Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.</p>


SCIEX: LC-MS/MS fundamentals webinar series 2.0

Excel strategies for mass spectrometry data analysis

Elevate your analytical expertise with advanced techniques and workflows.

<p><strong>Explore the latest knowledge on the safety, authenticity and quality of food and beverages, as well as the technology that supports the critical work done to ensure the integrity of the food supply chain.</strong></p>
<h5>This online symposium will explore:</h5>
<ul>
<li><strong>Untargeted analysis techniques used to ensure food authenticity</strong></li>
<li><strong>Sample preparation techniques for food applications</strong></li>
<li><strong>Use of thermal infrared imaging for salmonella detection</strong></li>
<li><strong>Elemental analysis for food and beverage testing</strong></li>
<li><strong>Miniaturized food analysis devices</strong></li>
<li><strong>Sustainable lab consumables for food analysis</strong></li>
<li><strong>Heavy metals in processed baby food</strong></li>
<li><strong>Residual analysis using GC/MS</strong></li>
</ul>
<h3><strong>Day 2 – July 25</strong></h3>
<h5><strong>16:00 – 16:40 BST Miniaturization of Analytics: A Better Control from Farm to Fork</strong></h5>
<ul>
<li>Marta Prado, PhD (University of Santiago de Compostela)</li>
</ul>
<h5><strong>16:40 – 16:50 BST</strong> Networking Break</h5>
<h5><strong>16:50 – 17:10 BST Unleashing the Power of AI powered LIMS in Food and Beverage Testing</strong></h5>
<ul>
<li>Daniel Call (LabVantage)</li>
</ul>
<h5><strong>17:10 – 17:20 BST</strong> Networking Break</h5>
<h5><strong>17:20 – 18:00 BST Heavy Metals in Processed Baby Food: Could Regulators be Doing More to Ensure Consumer Safety?</strong></h5>
<ul>
<li>Robert Thomas (Scientific Solutions)</li>
</ul>
<h5><strong>18:00 – 18:10 BST</strong> Networking Break</h5>
<h5><strong>18:10 – 18:30 BST Sustainable Lab Consumables without Compromising Performance</strong></h5>
<ul>
<li>Jack Gikonyo (Eppendorf Americas)</li>
</ul>
<h5><strong>18:30 – 18:40 BST</strong> Networking Break</h5>
<h5><strong>18:40 – 19:20 BST Applications of NIRS and NIR Imaging in Food</strong></h5>
<ul>
<li>Vincent Baeten, PhD (Walloon Agricultural Research Centre)</li>
</ul>
<h5><strong>19:20 BST</strong> Event Close</h5>


Technology Networks: Advances in Food and Beverage Testing 2024

Advances in Food and Beverage Testing 2024 (Day 2)

Explore the latest knowledge on the safety, authenticity and quality of food and beverages.

<h4><strong>SCIEX OS software summer school</strong></h4>
<p><strong>Master the essentials of SCIEX OS software with our live webinars this summer</strong></p>
<p>Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software. Over the course of next months, we offer a series of concise, 30-minute webinars designed to fit into your schedule.</p>
<p>Agenda:</p>
<ul>
<li>23 July, 11:00 a.m. CET – Hardware configuration and instrument verification</li>
<li>6 Aug, 11:00 a.m. CET – Compound optimization</li>
<li>20 Aug, 11:00 a.m. CET – Data processing: quantitation</li>
<li>2 September, 11:00 a.m. CET – Data processing: screening</li>
</ul>


SCIEX OS software summer school - Compound optimization

Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software.

Compound optimization

SCIEX OS software summer school - Data processing: quantitation

Data processing: quantitation

SCIEX OS software summer school - Data processing: screening

Data processing: screening

<p><strong>Digitalizing laboratory sample workflows is crucial yet challenging due to complex data types, resource constraints, and the need for integration, automation, training, and more.</strong></p>
<p>But with CaliberLIMS, these challenges become opportunities. CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.</p>
<p>With the upgraded version, CaliberLIMS 4.0 enables users to effortlessly adopt a futuristic workflow that integrates instruments, workflows, and data across the entire laboratory. Packed with powerful features, this advanced software can enhance compliance, integrity, and productivity, making your laboratory tomorrow-ready.</p>
<p>Register now to secure your spot!</p>
<p><strong>Learning Objectives:</strong></p>
<ul>
<li>Learn how LIMS application can accelerate laboratory efficiency </li>
<li>Explore how digitalization can be made easier with scalable solutions </li>
<li>Understand how to achieve seamless integrations with CaliberLIMS for automated workflows</li>
<li>Discover the latest advancements in Caliber 4.0</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Kanimozhi Sivarajan (Informatics Manager, Shimadzu (Asia Pacific) Pte Ltd)</p>
</blockquote>
<blockquote>
<p>Dr. Kani is having 10 years of experince in Laboratory Management and Informatics  Systems. She is serving as Informatics Manager at Shimadzu Asia Pacific, Singapore,  providing Lab Informatics Solutions such as LabSolutions CS, LIMS and other QMS applications. She is supporting customers from various segments on implementing laboratory automation and responsible for providing consultation on streamlining Lab Workflows, Regulatory Compliance requirements for Electronic Data Management and Lab Practices/Analysis. </p>
</blockquote>


Shimadzu: Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence

Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence

CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.

 Pharma 4.0: CaliberLIMS For Digital Excellence

<p><strong>Does regulated laboratory software use in a modern software ecosystem have to be complex?</strong></p>
<p>Join us for our latest webinar to unlock insights into the intricacies of regulated laboratory software. We will discuss the changing regulatory landscapes, observations, and considerations when aligning software records with ALCOA+ principles as well as the essentials of requirements, qualification and validation for laboratory software. If you work in QC, or are working in a regulated laboratory, you do not want to miss this!</p>
<p>Learning Objectives:</p>
<ul>
<li><p><strong>Review the dynamics of regulatory changes impacting modern software ecosystems</strong></p>
</li>
<li><p><strong>Discover the significance of aligning software records with ALCOA+ principles for data integrity and compliance</strong></p>
</li>
<li><p><strong>Identify and gain insights into the requirements, qualification, and validation processes essential for effective laboratory software management</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Joe Ault (Sr. Product Marketing Manager, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>Joe Ault has a diverse professional background in pharma, having contributed significantly to validation campaigns for both small-scale, gene therapy startups and large pharma firms. Previously, Joe was a senior manager of computerized system validation at a cell and gene therapy startup, where his focus was on commercialization. He has also held roles as a computerized system validation engineer focused on laboratory and manufacturing systems. In addition to his roles at pharmaceutical firms, Joe has served as a validation consultant for industry leaders specializing in chromatography data systems. In this capacity, he played a crucial role in ensuring the validation and compliance of these systems, contributing to the reliability and accuracy of critical data for regulated customers. With a career spanning commercialization in startups and validation expertise of chromatography data systems, Joe is now focused on the creation and delivery of validation services in support of Thermo Fisher Scientific customers of enterprise software products.</p>
</blockquote>


Thermo Scientific: Navigating changes in regulated software environments

Navigating changes in regulated software environments

Changing regulatory landscapes, observations, and considerations when aligning software records with ALCOA+ principles.

<p><strong>Delivering connected science for your lab evolution</strong></p>
<p>In the fast-paced world of laboratory operations, staying ahead of regulatory changes, increasing demands, and fierce competition is essential. However, outdated instrumentation and software can often hinder your progress.</p>
<p>Our newly launched software is meticulously crafted to elevate laboratory efficiency and ensure reproducibility, all while minimising manual errors. One of the core tenets of our software is its user-friendly interface, designed with simplicity in mind. Here’s your opportunity to get firsthand experience!</p>
<h3><strong>AGENDA</strong></h3>
<h5><strong>09:00 Welcome + Coffee + Networking</strong></h5>
<h5><strong>09:30 Introduction to waters_connect: Delivering connected science for your lab evolution</strong></h5>
<h5><strong>09:45 Streamlining System Setup and Data Acquisition: Maximizing efficiency and accuracy</strong></h5>
<h5><strong>10:30 Coffee and networking opportunities</strong></h5>
<h5><strong>11:00 Compound optimization with just a few clicks - Hands-on workshop</strong></h5>
<h5><strong>11:45 Mastering MS Quan: Demonstration of next generation of data processing</strong></h5>
<h5><strong>12:30 Lunch</strong></h5>
<h5><strong>13:30 Mastering MS Quan: Unleash your analytical potential- hands-on workshop</strong></h5>
<h5><strong>15:30 General discussion, coffee and wrap-up</strong></h5>


Waters Corporation: waters_connect for Quantitation workshop

waters_connect for Quantitation workshop

Here’s your opportunity to get firsthand experience.

<p><strong>We are excited to bring you a three-part Ask Me Anything webinar series on one of the topics you ask about most – processing data. Empower Software allows you to use two different peak detection algorithms, Traditional and ApexTrack. What are the differences between these algorithms and when might you use one versus the other? Given all the choices on the Integration tab of the Processing Method, how do you set these important parameters to ensure accurate peak integration? &nbsp;</strong></p><p>In the first part of this webinar series, we will explore the algorithms and learn how to set the parameters in a logical fashion. <strong>We will discuss:</strong></p><ul><li>How both algorithms work</li><li>Starting with peak width and threshold first</li><li>Interactively setting timed events in the chromatogram</li><li>Practical examples</li><li>Empower System Policies that impact your peak detection and integration</li></ul><p>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p></blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 1

Empower Tips: Ask Me Anything About Processing Data - Part 1

We will explore the algorithms and learn how to set the parameters in a logical fashion.

Empower Tips: Ask Me Anything About Processing Data

<p><strong>Method development and validation is a time-consuming process that requires the attention of experienced laboratory personnel. There are tools that integrate with Empower Software that dramatically reduce the time needed to build methods, evaluate results, and validate the final method. With these tools you can free up experienced laboratory personnel to carry out other tasks. These tools are compatible with older versions of Empower Software if you have Windows 10.</strong></p><p><strong>In this webinar we will discuss:</strong></p><ul><li><strong>Method Developer Application</strong> – A new autonomous tool for method screening</li><li><strong>Sample Set Generator </strong>– A tool that automates the creation of Instrument Methods, Method Sets and Sample Set Methods for method development and validation</li><li><strong>Method Validation Manager</strong> – An Empower Software option that automates chromatographic method validation and performs all necessary statistical calculations</li><li><strong>Scoring Report</strong> – A Report Method that scores and ranks the results of method development experiments making it easy to select the optimum separation</li><li><strong>Third-Party AQbD Software</strong> – Software that integrates seamlessly with Empower Software to define the experimental region within which changes to method parameters will not significantly affect the separation</li></ul><p>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter: </strong>Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p></blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Tools for Method Screening, Development, and Validation

Empower Tips: Ask Me Anything About Tools for Method Screening, Development, and Validation

In this webinar we will discuss method Developer Application – A new autonomous tool for method screening, sample Set Generator etc.

LCMS webinars focusing on Software - page 24