<div><div><div>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in Nature Communications.</div></div><div>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916">As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en">Prof. Michal Hocek</a>, has now developed a technology that significantly shortens the development process.“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” says Marek Ondruš.<blockquote>Video:<a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></blockquote>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. “We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,” says Marek Ondruš.In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"><blockquote><h4>Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas">Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</a></h4>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal HocekNat. Commun., 2026<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y">https://doi.org/10.1038/s41467-026-73676-y</a>&nbsp;<h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity</a> 10.2 highlights</h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4>Instrument control modules</h4>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"><h4>MS data import/export improvements</h4>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"><h4>Obsolete hardware support terminated</h4>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.2.👉<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog&nbsp;</a></blockquote>

Clarity Chromatography Software 10.2 released!

🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

Article

Product

Video

Scientists

Environment

Water

Interview

Science and research

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Agilent Technologies

Shimadzu Corporation

Waters Corporation

When it comes to LC analysis, a common question for users is, “Which LC column should I choose?” The column is the heart of the LC system, which plays a pivotal role in ensuring high-resolution separation. The fundamental knowledge of column selection can hence help you decide the optimal column for your specific application.
In this second series of Understanding LC Columns webinar, we will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC. We will discuss some of the common applications for each of these separation techniques. The difference in column chemistry and column specifications can significantly impact your separation. This webinar will also cover the different column chemistry and the considerations when selecting a column for the different applications.
Learning Objectives
<ul>
<li>Understand the different mode of separation and their application in liquid chromatography.</li>
<li>Understand the different column chemistry and their separation mechanisms.</li>
</ul>
<blockquote>
Presenter: Dr. Derrick Tan (Application Chemist (Consumables), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Dr. Derrick Tan obtained his PhD in chemistry from Nanyang Technological University, Singapore. He has more than 7 years of research experience in small molecules, nucleic acids, peptides and protein synthesis, separation and purification. He joined Shimadzu (Asia Pacific) Pte Ltd in 2021 as an Application Chemist (Consumables). At Shimadzu, he is responsible for the technical support and application of consumables in chromatography and spectroscopy. Products include LC and GC columns, UV/Vis cuvettes, sample vials and accessories.
</blockquote>

Shimadzu: Understanding LC Columns - Difference in Column Types and Phases

Understanding LC Columns - Difference in Column Types and Phases

We will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC.

Understanding LC Columns - Column Types and Phases

In UV-VIS-NIR spectroscopy, there is a wide range of instruments available that cover all measurements from routine to research.
We will discuss in this presentation some of the key performance differentiators between spectrometers as well as the impact that these have on measurement capabilities.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Picking the Right UV-Vis-NIR For Me: What Specs Do I Need?

Picking the Right UV-Vis-NIR For Me: What Specs Do I Need?

Key performance differentiators between UV-VIS-NIR spectrometers as well as the impact that these have on measurement capabilities.

How do I collect a UV-VIS Baseline? Why is my concentration curve non-linear? What amount of averaging do I need to do?
Join us in this webinar where we will discuss some of the most common questions for UV-VIS data collection.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, gilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Common UV-Vis Questions Answered

Common UV-Vis Questions Answered

Join us in this webinar where we will discuss some of the most common questions for UV-VIS data collection.

CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES
This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins. Understanding the fundamentals of bioseparations will enable you to develop methods more efficiently. At the end of this series, you will be up to date on cutting edge principles and innovations surrounding bioseparations.
Who should attend? 
<ul>
<li>Those interested in a refresher on foundational principles and tips for bioseparations </li>
<li>Those revisiting which columns and mobile phases are optimal for which workflows </li>
<li>Those looking for an overview of industry advancements and new offerings</li>
</ul>
<blockquote>
Presenter: Bill Warren (Principal Product Manager – Chemistry Technology Center, Waters Corporation)
</blockquote>
<blockquote>
Bill received his Masters in Microbiology and Immunology from University of Louisville School of Medicine. In 1986, he joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Following four years as a field based technical sales representative for Waters, Bill enjoyed a tenure as Manager, Core Product Evaluation Laboratory within the Waters Worldwide Marketing Organization. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.
</blockquote>
<blockquote>
HOST: Zerene Manaloto (Chemistry Products Marketing Manager, Waters Corporation)
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<blockquote>
Zerene is a Chemistry Product Marketing Manager within the Americas Field Marketing team. She holds a Chemical Engineering Bachelor’s degree from Stevens Institute of Technology. Early in her career, Zerene was a research scientist at Bristol-Myers Squibb in Process Research &amp; Development involved in preparative scale purification and glass plant operations to support IND and clinical supplies of drug candidates. Prior to joining Waters, Zerene was in consumable field sales, supporting key accounts and customers within the pharmaceutical and clinical industries.
</blockquote>

Waters Corporation: CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS

This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins.

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS 

Christian Rohrer, Director Sales and Marketing at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.
On Tuesday, February 14, Andrea Krumm, Product Manager Analytical Columns at Tosoh Bioscience, will present how powerful Hydrophobic Interaction Chromatography is in analyzing proteins.
You will learn how:
<ul>
<li>HIC works
</li>
<li>HIC methods are developed
</li>
<li>HIC is applied to analyze various protein modifications
</li>
</ul>
<blockquote>
Presenter: Christian Rohrer (Director Sales and Marketing at Tosoh Bioscience GmbH)
</blockquote>

Tosoh Bioscience: Tosoh Tuesday - HIC for protein analysis: why, what, and how?

HIC for protein analysis: why, what, and how?

How powerful Hydrophobic Interaction Chromatography is in analyzing proteins.

This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.
<ul>
<li>What is LC purification and how do you get the most out of the technique.
</li>
<li>How much sample can you expect to purify?
</li>
<li>Up-scaling basics
</li>
<li>Recycling and concentration
</li>
</ul>
<blockquote>
Presenter: Richard Hodgson (Pharmaceutical Market Specialist, Shimadzu UK Limited)
</blockquote>

Shimadzu: Fundamentals of LC Purification

Fundamentals of LC Purification

What is LC purification and how do you get the most out of the technique.

Your new Cary 3500 UV-Vis has arrived, been installed, and now you need to collect data. This presentation will walk you through various applications such as Scan, Kinetics, and Thermal Melts and the process of setting up instrument parameters and collecting data.

Agilent Technologies: Agilent Webinars On Demand

Scans, Kinetics, and Thermal Melts: An UV/Vis Application Workshop

Cary 3500 UV-Vis - We will walk you through various applications such as Scan, Kinetics, and Thermal Melts and the process of setting up instrument parameters

Scans, Kinetics, and Thermal Melts: An UV/Vis Application 

Cannabis is a new and popular industry with potential for exponential growth over the coming decade. As the industry continues to mature, regulations surrounding its quality are expected to become more stringent.&nbsp;This webinar will discuss the basic principles needed to ensure that laboratory data are both compliant and defensible according to ISO 17025 and current Good Manufacturing Procedures (cGMP). The webinar will also cover the differences between method validation, verification, and modification. The instrumental method validation process will be reviewed step-by-step and will include the documentation necessary for methods to be approved by ISO-17025:2017 auditors as well as most other regulatory bodies. Tips and tricks for successful validations are provided.&nbsp;Topics covered include:<ul><li>Data Integrity</li><li>Instrument Qualifications</li><li>Method Validation</li><li>Method performance parameters like accuracy, precision, linearity, LOD, LOQ, and robustness</li><li>ISO-17025:2017 Documentation</li></ul>Key Learning Objectives:<ul><li>Fundamentals of data integrity</li><li>Importance of instrument qualifications</li><li>Understand why and when a method needs to be validated</li><li>Elements of method validation</li><li>Tips and tricks in method validation</li></ul>Who Should Attend:<ul><li>Laboratory personnel working in compliant laboratories and/or requiring reliable and accurate analysis data.</li><li>Testing facilities of cannabis-derived products (Hemp or Marijuana)</li><li>ISO 17025 accredited labs</li></ul>In case of any technical questions. please contact Martha Devia: <a href="mailto:Mdevia@mjhlifesciences.com">Mdevia@mjhlifesciences.com</a><blockquote>Presenter: Donna Payne (Analytical Chemist, Quality Systems Manager ISO 17025:2017 Certified Food Lab, CEO AnalytEval)Growing up in South Florida, Donna Payne wanted to be a scientist from age 9. She graduated from the University of Miami with a BS in Chemistry. Donna attended graduate school at the University of South Florida in Tampa, earning an MS degree in Physical Analytical Chemistry. She gained invaluable experience in Quality Assurance working on the manned space program as well as other aerospace and defense programs. Donna spent 17 years as a professor of Chemistry at two colleges in the Houston, Tx, area and has managed a private, ISO-17025:2017 certified analytical food lab for the last 6 years. She specializes in analytical method development and method validation.Presenter: Matt Abrahms (Compliance Application Expert, Agilent Technologies, Inc.)Matt Abrahms is a laboratory compliance specialist focused on instrument qualifications and computer system validation. He is a frequent speaker at Agilent’s compliance seminars/webinars and has authored multiple whitepapers. He has performed over 100 instrument qualifications in GMP environments and works closely with Agilent’s customers to comply with new regulatory updates. As a specialist he collaborates with Agilent’s solutions unit and service teams to ensure the quality of Agilent’s compliance delivery.</blockquote>

How to Ensure Laboratory Compliance in Your Cannabis Testing Lab

The basic principles needed to ensure that laboratory data are both compliant and defensible according to ISO 17025 and cGMP

How to Ensure Laboratory Compliance in Your Cannabis Lab

The best HPLC method is one that produces reproducible analytical results under actual lab conditions. To produce results that are accurate and consistent over the long term the HPLC separation must either be relatively insensitive to small variations in analysis conditions, or those variations must be identified and controlled satisfactorily.&nbsp;The development of a robust method is not only cost effective by reducing wasteful lab downtime, it also increases the likelihood of successful method transfer to other in-house operations, or contract analytical laboratories.This presentation will:<ul><li>demonstrate how to determine the controlling parameters in a separation</li><li>evaluate proper operating range of those parameters</li><li>control strategy for those parameters</li><li>facilitate faster method development and validation</li></ul>By learning how to investigate and control the important factors in HPLC separations, attendees will be able to develop good, new methods for new products that are brought to market. As in any process that analyzes material for public consumption, improved analytical methods minimize unplanned downtime, increase lab efficiency and safe-guard the quality of those products. Less Downtime-More Sample Throughput.<blockquote>Presenter: John Palmer (LC Column Application Engineer, Agilent Technologies, Inc.)<ul><li>B.S. degree in chemistry from the University of Cincinnati in 1977.</li><li>Perkin Elmer GC and HPLC Specialist from 1984-1986.</li><li>MAC-MOD Analytical in 1986 as a Chromatography Specialist for Zorbax HPLC Columns. His primary assignment was to aid customers in improving HPLC applications.</li><li>Agilent Technologies in 2001 to continue to deliver chromatography support for the Agilent ZORBAX separation products.</li></ul>John’s primary interest is enhancing the utility and robustness of HPLC/UHPLC separations by practical application of theory to everyday laboratory practice. His current interests are in high speed and high resolution separations of small molecules and bio-molecules utilizing both conventional HPLC and UHPLC technology.He has collaborated on several papers and presentations including “Development and Validation of Analytical Methods in Pharmaceutical and Biomedical Research” for Pharmaceutical and Biomedical Applications of Liquid Chromatography (Riley, Lough, and Wagner).</blockquote>

Eliminating Unwanted Variability in LC Methods: More Uptime, More Sample Throughput

We'll show you how to investigate and control the important factors in HPLC separations and develop new methods for new products that are brought to market

Eliminating Unwanted Variability in LC Methods

The Importance of Reliable THC Concentration and the Role of Analytical Data.<blockquote>Presenter: Jodi McDonald (President, Keystone Labs Inc.)</blockquote>

LCMS webinars focusing on Academy/Principles - page 24

HIC for protein analysis: why, what, and how?

CHROMATOGRAPHY CONCEPTS FOR PEPTIDES AND PROTEINS

Picking the Right UV-Vis-NIR For Me: What Specs Do I Need?

Common UV-Vis Questions Answered

Understanding LC Columns - Difference in Column Types and Phases

Fundamentals of LC Purification

The Importance of Reliable THC Concentration and the Role of Analytical Data

Scans, Kinetics, and Thermal Melts: An UV/Vis Application Workshop

How to Ensure Laboratory Compliance in Your Cannabis Testing Lab

Eliminating Unwanted Variability in LC Methods: More Uptime, More Sample Throughput

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