<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Waters Corporation

<h3><strong>Solving Problems That Matter - a Waters webinar series featuring USP speakers</strong></h3>
<h4><strong>Episode 5: Impurity Profile Characterization</strong></h4>
<p><strong>Summary</strong></p>
<p>Characterizing of a product’s impurity profile refers to the process of establishing the impurities and degradation products (both actual and potential) that are most likely to arise during the synthesis, purification, and storage of a drug substance and additionally during the manufacturing and storage of a drug product. This session will explore how LC with optical and mass detection can be employed for impurity profiling.</p>
<p><strong>Key learning objectives:</strong></p>
<ul>
<li>Understand the analytical challenges related to organic impurity profile characterization</li>
<li>Learn about the benefits of adding mass detection</li>
</ul>
<p><strong>Who should attend:</strong></p>
<p>GMP laboratory analysts and managers involved with organic impurity profile characterization</p>
<blockquote>
<p><strong>Presenter</strong>: Christian Zeine (US Pharmacopeia)</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Henry Foddy (Associate Scientist, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Henry is a Scientist in the Routine Mass Detection Team at Waters, working on applications to support Waters’ single quadrupole portfolio that span a variety of market segments. He has almost 3 years of experience with Waters, working previously in a food and environmental role support regulatory testing of Pesticides, PFAS, and Pyrrolizidine Alkaloids. Prior to joining Waters, Henry obtained an MSc in Neuroscience from King’s College London.</p>
</blockquote>
<blockquote>
<p><strong>Moderated by</strong>: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</p>
</blockquote>


Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Impurity Profile Characterization

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 5: Impurity Profile Characterization.

<p><strong>PhenoAcademy Webinars</strong></p>
<p> <strong>Liquid Chromatography Series</strong></p>
<h4><strong>HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns</strong></h4>
<ul>
<li>Recognize columns exhibiting comparable selectivity to your current column selection</li>
<li>Identify columns with different or orthogonal selectivity to your current column of choice</li>
<li>Understand the resources that are available for selectivity data for HPLC columns</li>
</ul>
<blockquote>
<p><strong>Presenter</strong>: Kevin Zhao (Senior Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Koen Askamp (Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?</p>
</blockquote>
<blockquote>
<p>I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Namrata Saxena (Technical Manager)</p>
</blockquote>
<blockquote>
<p>Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. </p>
</blockquote>
<blockquote>
<p>Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. </p>
</blockquote>
<blockquote>
<p>In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally.  </p>
</blockquote>


Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Recognize columns exhibiting comparable selectivity to your current column selection.

Identification of Equivalent and Orthogonal HPLC Columns

<h4><strong>European Free Educational Webinar Series</strong></h4>
<p><strong>Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.</strong></p>
<h4><strong>EPISODE 3</strong></h4>
<p><strong>Theory of Liquid Chromatography</strong></p>
<p><strong>What will you learn?</strong></p>
<ul>
<li>Theory of Chromatography</li>
<li>Creating great Separation Methods</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Tobias Langrock (Senior Application Specialist Chemistry Europe, Waters)</p>
</blockquote>
<blockquote>
<p>During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.</p>
</blockquote>
<h4><strong>What&#39;s next?</strong></h4>
<p>Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652"><strong>here</strong></a></p>
<p>Back to Basics - Sample Preparation: Theory and Overview</p>
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple</p>
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Theory of Liquid Chromatography</p>
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Basics of Liquid Chromatography</p>
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Analytical Method Development</p>
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - The importance of Compliance</p>
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Introduction to Mass Spectrometry</p>
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
<p>Back to Basics - Troubleshooting your LC and MS Systems</p>
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
<p><strong>Not sure to be able to attend live sessions?</strong></p>
<p>Register today and watch the webinars on-demand</p>


Waters Corporation: Back to Bacisc Free Educational Webinar Serries

Theory of Liquid Chromatography

 The theory of chromatography and how to create great separation methods.

<p><strong>Sample-to-sample and run-to-run reproducibility in chromatography is of utmost importance.</strong></p>
<p><strong>What do you do when your chromatography suddenly changes?</strong></p>
<p>In this webinar, we’ll discuss some of the most common chromatography issues that we see as technical support specialists. Whether you have retention time changes, pressure changes, new peaks, ghost peaks, or peak shape changes, troubleshooting these issues can be a challenge. We’ll provide explanations for why these changes may be happening and make recommendations for how to avoid them in the future.</p>
<blockquote>
<p><strong>Presenter:</strong> Jean Lane (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.</p>
</blockquote>


Agilent Technologies: My Chromatography Has Changed: Steps for Effective Troubleshooting

My Chromatography Has Changed: Steps for Effective Troubleshooting

We’ll discuss some of the most common chromatography issues that we see as technical support specialists.

Steps for Effective HPLC Troubleshooting

<h4><strong>Waters 2024 LC Symposium</strong></h4>
<p><strong>Pushing the Boundaries of LC to Address Today’s Challenges</strong></p>
<h5><strong>Overview</strong></h5>
<p>Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. </p>
<p>Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. </p>
<h3><strong>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</strong></h3>
<h5><strong>Advanced LC Technologies for Increasingly Complex Molecules</strong></h5>
<p>Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.</p>
<h5><strong>Learning objectives</strong></h5>
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
<p>Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.</p>
<blockquote>
<h3>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Next Generation HPLC: the Quest for Performance and Simplicity</h5>
</blockquote>
<blockquote>
<p>Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)</p>
</blockquote>
<blockquote>
<p>Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).</p>
</blockquote>
<blockquote>
<p>He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)</p>
</blockquote>
<blockquote>
<p>Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)</p>
</blockquote>
<blockquote>
<p>Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</p>
</blockquote>


Waters Corporation: Waters 2024 LC Symposium

Advanced LC Technologies for Increasingly Complex Molecules

Free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges.

<h4><strong>Waters 2024 LC Symposium</strong></h4>
<p><strong>Pushing the Boundaries of LC to Address Today’s Challenges</strong></p>
<h5><strong>Overview</strong></h5>
<p>Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. </p>
<p>Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. </p>
<h3><strong>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</strong></h3>
<h5><strong>Next Generation HPLC: the Quest for Performance and Simplicity</strong></h5>
<p>Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.</p>
<p><strong>Learning objectives</strong></p>
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
<p>Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.</p>
<blockquote>
<h3>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Advanced LC Technologies for Increasingly Complex Molecules</h5>
</blockquote>
<blockquote>
<p>Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)</p>
</blockquote>
<blockquote>
<p>Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).</p>
</blockquote>
<blockquote>
<p>He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)</p>
</blockquote>
<blockquote>
<p>Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)</p>
</blockquote>
<blockquote>
<p>Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</p>
</blockquote>


Next Generation HPLC: the Quest for Performance and Simplicity

Next Generation HPLC: Performance and Simplicity

<p><strong>Join us for thought provoking webinar series on the entire value chain of therapeutic peptides.</strong></p>
<p><strong>Register now and get an opportunity to learn about:</strong></p>
<ul>
<li>Peptide manufacturing specifications</li>
<li>Raw materials and intermediates</li>
<li>USP general chapters and regulatory considerations</li>
<li>Chromatographic strategies for optimizing peptide analysis and purification with reversed phase chromatography and suitable stationary phases</li>
<li>End-to-end analytical to preparative workflow for synthetic peptides</li>
</ul>
<h5><strong>Untold Control Strategies in Complex Peptide Manufacturing</strong></h5>
<ul>
<li>Dr. Vasanthakumar G. Ramu</li>
</ul>
<h5><strong>Regulatory Considerations for Peptides Therapeutics</strong></h5>
<ul>
<li>Dr. Manoj Kumar Metta</li>
</ul>
<h5><strong>Analysis and Purification of Synthetic Peptides</strong></h5>
<ul>
<li>Ms. Paramjeet Khandpur</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Vasanthakumar G. Ramu (Vice President – Head R&amp;D, Peptides and Complex API’s, Alembic Pharmaceutical Ltd.)</p>
</blockquote>
<blockquote>
<p>Dr. Vasanthakumar Ramu, is one of the pragmatic scientists in the pharma industry working around complex API’s. He studied chemical biology from Bangalore University and Indian Institute of Science. In early 2007, hemoved to Max-Planck Institute of Molecular Physiology to work on peptide decorated supramolecular architectures as modulators to study cell protein interactions. Continued further studies as research scientist in Ruhr Universität Bochum, Germany, with the objective to work on backbone modified peptides of HIV protease inhibitors. In 2011 spring, moved to Spain, where he was working as Marie Curie Experienced Researcher to develop Kyotorphin derived peptides, antimicrobial peptides, and other small molecules for neurodegeneration. Returned to India in 2012 and led the startup company called Omgene Life Sciences Pvt. Ltd. Before joining Alembic, was heading peptide process development groups in Biocon Ltd., and Aurobindo Pharma Ltd., Transferred the technologies, successfully validated and filed various DMF’s and involved in commercialization of API’s.</p>
</blockquote>
<blockquote>
<p>His work was centered on developing diverse peptides and complex therapeutic API’s to treat T2D and various other health ailments. Published more than 30 peer reviewed scientific articles and 50+ patent applications filed/granted in various IP jurisdictions. Dr. Vasanthakumar’s efforts have been recognized through Marie Curie and Max Planck Institute fellowships. He is fellow of Chemical Research Society of India (CRSI) and Nuclear Magnetic Resonance Society of India (NMRS) and recently, he has been invited as Fellow of Royal Society of Chemistry, London in leadership category in recognition of his contributions in chemical sciences. Presently, heading R&amp;D, and leading end to end development and technology transfer activities for various peptides and complex generic API’s.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Manoj Kumar Metta (Team Lead, Senior Scientist II Global Biologics, United States Pharmacopeia (USP))</p>
</blockquote>
<blockquote>
<p>Dr. Manoj Metta is a renowned Biological Scientist with 21 years of experience in Biologics Quality assessment, and Regulatory sciences. He has rich hands-on experience in commercial development of protein, and peptide biopharmaceutical products with emphasis on developing analytical control strategies, physicochemical and functional characterization, analytical method development/validations and CMC review to support product licensure in global markets.</p>
</blockquote>
<blockquote>
<p>Dr. Manoj brings a wealth of experience and expertise to his role at the US Pharmacopeia (USP), where he has served for over 12 years in various scientific capacities. His contributions span across multiple areas, including the development of Monographs, Reference standards and General chapters in therapeutic proteins, monoclonal antibodies, synthetic peptides, and oligonucleotides. Dr. Manoj is a frequent speaker at national and international biopharmaceutical and regulatory scientific events.</p>
</blockquote>
<blockquote>
<p>Academically, he received Ph.D. degree in Biotechnology from Gandhi Institute of Technology and Management, on “Development of novel mammalian expression systems by using polyomaviral elements in CHO cell line” for monoclonal antibody production”.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Ms. Paramjeet Khandpur (Biopharma Workflow Commercialization Lead. Agilent Techologies)</p>
</blockquote>
<blockquote>
<p>Paramjeet Khandpur, M.Tech., holds a bachelor’s degree in chemical engineering from VTU University and a Master&#39;s in Bioprocess Technology from the Institute of Chemical Technology, Mumbai. Paramjeet is currently the Biopharma Workflow Commercialization Lead at Agilent. In this role, she leverages her expertise to deliver comprehensive workflow solutions and offer support to customers, catering to their analytical requirements across the Biopharma value chain.</p>
</blockquote>
<blockquote>
<p>Previously, she worked as an Application Engineer at Agilent&#39;s Centre of Excellence Lab in Bangalore, specializing in various liquid chromatography platforms. She also collaborated with users in the Pharma and Biopharma industry for method development, troubleshooting, and method transfer.</p>
</blockquote>


Agilent: Advance Your Peptide Product Commercialization: CMC and Regulatory Considerations

Advance Your Peptide Product Commercialization: CMC and Regulatory Considerations

Webinar series on the entire value chain of therapeutic peptides.

Peptide Product Commercialization: CMC and Regulatory

<p><strong>This webinar unveils the development of a high-throughput multidimensional liquid chromatography approach in combination with charged aerosol detection (CAD), leveraging a combination of ion exchange and reversed-phase columns, ensuring accurate and precise analysis within minutes.</strong></p>
<p>Discover how this method advances formulation development, stability testing, and batch release, providing researchers with valuable insights into surfactant degradation dynamics. </p>
<p>Learning Points: </p>
<ul>
<li><strong>Discover cutting-edge techniques for rapid analysis of surfactants in antibody formulations</strong></li>
<li><strong>Unveil high-throughput liquid chromatography with charged aerosol detection</strong></li>
<li><strong>Gain valuable insights for optimizing formulation development and stability testing</strong></li>
</ul>
<p>Who should attend?</p>
<ul>
<li><strong>Biopharmaceutical scientists and researchers specializing in monoclonal antibody development</strong></li>
<li><strong>Analytical chemists and technicians involved in pharmaceutical formulation analysis</strong></li>
<li><strong>Quality control and regulatory affairs professionals in the pharmaceutical industry</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Maksymilian Zegota, PhD (Senior Scientist, Coriolis Pharma)</p>
</blockquote>
<blockquote>
<p>Dr. Zegota earned his PH.D. in chemistry from the Max Planck Institute for polymer research in Mainz, Germany, concentrating on site-selective protein modification.</p>
</blockquote>
<blockquote>
<p>After completing his doctorate, he joined Coriolis Pharma in Martinsried, Germany.</p>
</blockquote>
<blockquote>
<p>Currently, Dr. Zegota focuses on developing Novel methods for surfactant analysis in biologics to assist in the root-cause analysis of polysorbate and poloxamer degradation, contributing to formulation development in the pharmaceutical field.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Marcus Klee (Marcus Klee, Regional Marketing Manager HPLC, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>Graduated in Chemical Engineering from Hochschule Fresenius in 2000, specialized in Marketing &amp; Sales. Since then, held various marketing roles since 2000, currently serving as the Regional Marketing Manager EMEA for HPLC</p>
</blockquote>


Thermo Scientific: Advancing analytical techniques in monoclonal antibody formulations

Advancing analytical techniques in monoclonal antibody formulations

Explore cutting-edge methodology for online protein removal and characterization of polysorbates and poloxamer in monoclonal antibody formulations.

Analytical techniques in monoclonal antibody formulations

<p><strong>The bioprocessing industry has identified an urgent need to intensify downstream operations, making them smaller, faster, and more cost-effective. While numerous technologies and techniques exist to intensify individual unit operations, implementing an integrated next-generation bioprocess presents significant challenges. Our collaboration with Transcenta has culminated in the development of a state-of-the-art, highly automated bioprocessing train for the manufacture of monoclonal antibodies and other biologics.</strong></p>
<p>This webinar will detail the bioprocessing template, operational paradigm, and the complexities involved in qualifying such an advanced manufacturing method.</p>
<p><strong>In this webinar, you will learn:</strong></p>
<ul>
<li>What constitutes an intensified bioprocess,</li>
<li>Transcenta&#39;s template for next-generation bioprocess capability,</li>
<li>The installation and qualification of a multi-column chromatography system and a flow-through polishing system,</li>
<li>Strategies for managing and qualifying long-duration bioprocessing challenges, such as bioburden control.</li>
</ul>
<p><em>An on-demand version of this webinar will be available after the live event using the same link. Register now and access the webinar at your convenience.</em></p>
<blockquote>
<p><strong>Presenter:</strong> Jerome Dalin (Associate Director – Alliance Management, BioContinuum™ Business Franchise)</p>
</blockquote>
<blockquote>
<p>Jerome Dalin holds an MSc/Engineering degree in Industrial Manufacturing and Biological Engineering. He began his career at Sanofi Pasteur 21 years ago, focusing on vaccine process development in France, Canada &amp; USA. In 2013, Jerome joined our company to manage bioprocessing sales and later transitioned to the Strategy organization to lead global initiatives for vaccines and the Factory of the Future. In his current role, Jerome is at the helm of Alliance Management, driving the development of the BioContinuum™ platform across the biopharmaceutical industry.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Kara Pizzelli (Downstream Process Strategic Product Manager, Biocontinuum™ Business Franchise)</p>
</blockquote>
<blockquote>
<p>Kara Pizzelli holds a MSc degree in Chemical Engineering. Her career started at our company 25 years ago focusing on the optimization and scale-up of the unit operations from Clarification to Bulk Drug Filtration for customers in the US. Kara more recently managed the global Clarification business, owning the strategy and innovation, before taking on the role of DSP Strategic Product Manager in the Biocontinuum™ Business Franchise in 2023.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Chris Hwang (EVP, CTO, Transcenta)</p>
</blockquote>
<blockquote>
<p>Chris Hwang holds a Ph.D. in Biochemical Engineering from MIT and has over 30 years of experience in the biopharmaceutical industry. He began his career at Genzyme and later at Sanofi, supporting and leading CMC development as well as process and technology development. He also served as project lead for the Integrated Continuous Biomanufacturing platform program. Chris joined HJB/Transcenta in 2016, where he is responsible for CMC development and developing and industrializing highly intensified biomanufacturing platforms to increase facility output, enhance process control, and reduce the cost of goods to expand patient access to innovative biologics.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jewell Zhou (Lead Scientist – CMC Process Development, Downstream Platform, Transcenta)</p>
</blockquote>
<blockquote>
<p>Jewell Zhou holds a Msc/Engineer degree in Biology and Bioprocessing Engineering. He joined the HJB company in 2018 as a scientist to develop downstream purification process for CMC. Since 2020, he joined the HJB HiCB group and started to build and support continuous purification platform for advanced antibody manufacturing. In his current role, Jewell is working on HICB/Transcenta platform development and supporting continuous manufacturing control strategy.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Paul Beckett (Senior Strategic Product Manager – Clarification and Chromatography GTM Lead)</p>
</blockquote>
<blockquote>
<p>Paul holds a bachelor’s degree in biochemistry from Imperial College London and has a master’s degree and doctorate in biochemical engineering from University College London. Paul has worked in the pharmaceutical industry for 20 years and has held a number of roles in this capacity, including managing bioprocesses for early phase clinical material, CMC regulatory affairs consultancy and programme management of the next generation bioprocessing initiative. His current role is the global GTM lead for the chromatography and clarification technologies at our company.</p>
</blockquote>


Merck: Collaborative Bioprocessing: Next-Gen mAb Downstream Manufacturing Enhanced by Transcenta Partnership

Collaborative Bioprocessing: Next-Gen mAb Downstream Manufacturing Enhanced by Transcenta Partnership

This webinar will detail the bioprocessing template, operational paradigm, and the complexities involved in qualifying such an advanced manufacturing method.

Next-Gen mAb Manufacturing in Partnership with Transcenta

<p><strong>Advances in monoclonal antibody (mAb) engineering have led to additional challenges in the purification of these modalities, including high levels of aggregates. Ion exchange chromatography is often not sufficient to achieve purity targets meaning hydrophobic interaction chromatography (HIC) and mixed-mode resins are needed as orthogonal tools. These technologies can be implemented in flow-through mode allowing for high loadings and fast processing times, making them well-suited for large-scale implementation.</strong></p>
<p>This webinar highlights three case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics by reducing the levels of aggregates and host cell proteins (HCPs) and effectively clearing retroviruses.</p>
<p><strong>Attend this webinar to explore case studies on:</strong></p>
<ul>
<li>Effective aggregate removal and viral clearance via HIC flow-through unit operation</li>
<li>High molecular weight reduction and viral clearance for a novel, pre-conjugated mAb for ADC production</li>
<li>Simultaneous HCP reduction and aggregate removal with mixed-mode chromatography for a high aggregate mAb feedstream</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Robert Stairs (Field Applications Staff Scientist, Thermo Fisher Scientific)</p>
</blockquote>


Technology Networks: Advances in Flow-Through Technology To Enhance mAb Polishing

Advances in Flow-Through Technology To Enhance mAb Polishing

Case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics.

LCMS webinars focusing on HPLC - page 25