<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Technology Networks

Unchained Labs

C&EN

SCIEX

Evosep Biosystems

<h5><strong>Key Learning Points</strong></h5>
<ul>
<li><p>An overview of sample preparation strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix.</p>
</li>
<li><p>Find ways ensure effective sample preparation and cleanup methods are implemented.</p>
</li>
<li><p>Learn about method optimization for small volume sample extraction and clean-up solutions for oligonucleotides will be explored to further optimize challenging methods.</p>
</li>
</ul>
<h5><strong>Who Should Attend</strong></h5>
<p>Any Bioanalytical chemists new to working with oligonucleotides, or anyone new to oligonucleotide characterization by LC-MS</p>


Phenomenex: Novel Oligonucleotide Sample Clean-up and Method Optimizations

Novel Oligonucleotide Sample Clean-up and Method Optimizations

Sample preparation strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix.

Oligonucleotide Sample Clean-up and Method Optimizations

<p><strong>Tissue homogenization and cell lysis are crucial steps in protein extraction for LC-MS-based proteomics and many other research applications.</strong></p>
<p>During this webinar, you will discover ways to improve your protein extraction, such as by reducing workflow complexity, improving results from scarce samples, setting up robust assays and working with large sample cohorts. But why stop here?</p>
<p>We will also show how the full sample preparation workflow for LC-MS-based proteomics can be standardized and simplified with the BeatBox platform, which was developed for a wide variety of sample matrices and input ranges as well as how this can be applied to research such as cancer biomarker identification.</p>
<p>Attend this webinar to:</p>
<ul>
<li>Learn how to obtain reliable results through reproducible sample homogenization, even for challenging proteomics projects</li>
<li>Explore how to simplify and standardize the full sample preparation workflow for LC-MS-based proteomics</li>
<li>Discover how the BeatBox, a novel tissue homogenizer and cell lyser, can help you to efficiently extract proteins from a wide range of tissue and cell types</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Jasmin Johansson (Application Scientist, PreOmics)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Silvia Wuertenberger (Product Manager, PreOmics)</p>
</blockquote>


Technology Networks: Streamlining Protein Extraction for Proteomics and Beyond

Streamlining Protein Extraction for Proteomics and Beyond

You will discover ways to improve your protein extraction, such as by reducing workflow complexity, improving results from scarce samples ...

<p>Key learning objectives</p>
<ul>
<li><p><strong>Reversed Phase-Ion Pair LC for Oligos - Challenges and Optimization</strong></p>
</li>
<li><p><strong>Rapid Isolation of Oligo Therapeutics from Biological Samples</strong></p>
</li>
<li><p><strong>High-throughput, RPC Purification</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Mahesh Thondawada (Business Development Manager Biopharma/Biologic)</p>
</blockquote>


Analytical BioSolutions for Therapeutic Oligonucleotides

Reversed Phase-Ion Pair LC for Oligos - Challenges and Optimization...

<p><strong>Sepsis is a growing global challenge due to factors such as high mortality rate and the increasing prevalence of antimicrobial resistance.</strong></p>
<h4><strong>A single and rapid Zeno SWATH DIA proteomic analysis for concomitant identification and antibiotic resistance profiling of micro-organisms from positive blood cultures</strong></h4>
<ul>
<li><strong>Presenter:</strong> Iulia MACAVEI, PhD (student in Analytical Chemistry, Biological Analysis and Mass Spectrometry, University of lyon)</li>
</ul>
<p>Today, approximately 1.3 million people die each year worldwide from bacterial antimicrobial resistance (AMR). AMR is of great concern in the context of bloodstream infections (BSIs) when it evolves into sepsis. If MALDI-TOF has markedly shortened the delay of the pathogen identification step, AMR evaluation is still based on growth tests in the presence of antibiotics. With the aim of streamlining this turnaround time to less than 90min from a positive blood culture, the LC/Zeno SWATH DIA sensitivity and specificity performances were evaluated to concomitantly provide the identity and antibiotic resistance profile of etiological agents related to BSIs based on peptide surrogates.</p>
<h4><strong>Plasma proteomic landscape and patient heterogeneity in sepsis revealed by high-throughput mass spectrometry</strong></h4>
<ul>
<li><strong>Presenter:</strong> Dr. Yuxin Mi (Researcher at Wellcome Centre for Human Genetics, University of Oxford)</li>
</ul>
<p>Incomplete knowledge of the sepsis plasma proteome has limited our understanding of pathophysiology in the heterogeneous syndrome of sepsis. Here we apply high-throughput liquid chromatography – tandem mass spectrometry to delineate the plasma proteome for sepsis and comparator groups, involving 2622 samples in a single batch. We show how this scale of data can establish shared and specific proteins, pathways and co-expression modules in sepsis, and be integrated with paired leukocyte transcriptomic data. This work reveals novel subphenotypes informative for the response state and differential outcome, highlighting opportunities for a systems-based precision medicine approach to sepsis.</p>


SEPSIS

In this webinar we will uncover how our users combine technology with the Evosep One to further research into sepsis and its biomarkers.

Evosep SEPSIS

<p><strong>Get insight into topics ranging from the history of PFAS to analytical testing methods.</strong></p>
<p><strong>Day 1:</strong> Participate in an open discussion forum with our internal PFAS scientists from across the globe. Hear the story of how PFAS has shaped our environmental research. Take advantage of this digital space to share, learn and teach your fellow colleagues in the PFAS testing world.</p>
<p>Join this event to:</p>
<ul>
<li><strong>Learn about the history of PFAS and the need to increase testing for these “forever chemicals”</strong></li>
<li><strong>Understand how PFAS regulations differ across the globe</strong></li>
<li><strong>See what the future could look like in the PFAS testing world</strong></li>
<li><strong>Explore different matrices for PFAS testing</strong></li>
<li><strong>Discover how LC-MS can help solve challenges related to testing for PFAS chemicals</strong></li>
<li><strong>Get tips and tricks from seasoned PFAS testing experts</strong></li>
<li><strong>Find out what your peers are doing and share your knowledge in our open forum</strong></li>
</ul>
<p>This 2-day event is the first session of many PFAS summits SCIEX will host in the coming year, and there will be exciting activities for you to participate in throughout 2023. Join us for this first session and find out more about the next one: the in-person version!</p>
<h3><strong>Agenda Day 1</strong></h3>
<h5><strong>A brief history of PFAS</strong></h5>
<ul>
<li><strong>Dr. Craig Butt</strong> (Manager, Applied Market, SCIEX)</li>
</ul>
<h5><strong>Part 1: PFAS workflow overview: past, present and future</strong></h5>
<h5><strong>Part 2: The EMEAI perspective</strong></h5>
<ul>
<li><strong>Simon Roberts</strong> (Staff Application Scientist, SCIEX)</li>
<li><strong>Jianru Stahl-Zeng</strong> (Senior Technical Manager, EMEAI, SCIEX)</li>
</ul>
<h5><strong>PHX complementary workflow</strong></h5>
<ul>
<li><strong>Sam Lodge</strong> (Senior Business Development Manager, Phenomenex)</li>
</ul>
<h5><strong>Automating the Solid Phase Extraction (SPE) of PFAS for a range of methods and matrices</strong></h5>
<ul>
<li><strong>Ian Wan</strong> (Product Manager, PromoChrom Technologies)</li>
</ul>
<h5><strong>What does 4ppq actually look like?</strong></h5>
<ul>
<li><strong>Karl Oetjen</strong> (Senior Scientist, Technical Marketing, SCIEX)</li>
</ul>
<h5><strong>PFAS and customer support overview</strong></h5>
<ul>
<li><strong>Megumi Shimizu</strong> (LC-MS Application Scientist, SCIEX)</li>
</ul>
<h5><strong>An overview of different PFAS exposure pathways</strong></h5>
<ul>
<li><strong>Holly Lee</strong> (Food LC-MS Scientist, SCIEX)</li>
</ul>


PFAS summit: a scientific series (Day 1)

Join the discussion as SCIEX customers and industry experts explore how forever chemicals have shaped our environmental research.

<p><strong>Tangential flow filtration (TFF), also known as cross-flow filtration, is a rapid and efficient method for the separation and purification of biomolecules.</strong></p>
<p>TFF can be used to concentrate sample solutions ranging in volumes from 10 mL to thousands of liters. It can also be used to fractionate large from small biomolecules, harvest cell suspensions and clarify fermentation broths and cell lysates.</p>
<p>Another major usage of TFF is to perform a process called diafiltration, a technique that uses ultrafiltration membranes to completely remove, replace or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids and other biomolecules.</p>
<p>This webinar will explore the applications of diafiltration across immunology, protein chemistry, molecular biology, biochemistry and biotechnology.</p>
<p>Attend this webinar to learn:</p>
<ul>
<li>How to optimize laboratory-scale protein purifications</li>
<li>About tangential flow filtration techniques and ultrafiltration membrane selection</li>
<li>How to operate both continuous and discontinuous diafiltration methods</li>
</ul>
<p><strong>Presenter:</strong> Richard Pearce (Independent Consultant)</p>


Technology Networks: Simplified Diafiltration for Protein Purification

Simplified Diafiltration for Protein Purification

This webinar will explore the applications of diafiltration across immunology, protein chemistry, molecular biology, biochemistry and biotechnology.

<p><strong>The field of synthetic biology revolves around the design-build-test-learn (DBTL) cycle, where biological components are adjusted in multiple iterations. Automation allows synthetic biologists to spend less time pipetting liquids and devote more time understanding the science and finding creative solutions to problems.</strong></p>
<p>By combining automation for sample preparation with rapid separation methods we provide a phenotyping workflow suited to the needs of the synthetic biology community.</p>
<p>Attend this webinar to discover:</p>
<ul>
<li><strong>Automation solutions for synthetic biology applications</strong></li>
<li><strong>Rapid separation method workflows</strong></li>
<li><strong>How to improve confidence in annotation using ion mobility</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Tessa Moses (Metabolomics Specialist and Facility Manager of EdinOmics, Center for Engineering Biology, The University of Edinburgh)</p>
</blockquote>


Technology Networks: An Automated Workflow for Untargeted Metabolomics of Complex Biological Samples

An Automated Workflow for Untargeted Metabolomics of Complex Biological Samples

By combining automation for sample preparation with rapid separation methods we provide a phenotyping workflow for the synthetic biology community.

An Automated Workflow for Untargeted Metabolomics

<p><strong>The AssayMAP Bravo is a flexible yet easy-to-use automated micro chromatography-based protein sample preparation platform that leverages packed resin bed cartridges, precision flow control, and preoptimized applications to perform a wide variety of sample preparation workflows required during biotherapeutic development.  We have made several advances that allow the AssayMAP Bravo to address even more of the drug development process.  The system can now use large capacity cartridges, work in 21 CFR part 11 compliant labs, and automatically generate reports detailing the sample preparation steps executed.  These new capabilities enable walkup operation, simplified documentation, and seamless method transfer between labs so projects can be moved forward as quickly as possible.</strong></p>
<p>Key Learning Objectives:</p>
<ul>
<li>Discover how the AssayMAP Bravo makes automated sample preparation accessible to non-automation experts to reduce variability, human error, and manual labor.</li>
<li>Learn how new 21CFR part 11 enabling features in the new AssayMAP software enable walkup operation and simplify transfer of assays along the entire biotherapeutic development process, including moving from non-compliant to compliant labs.</li>
<li>Find out how documentation has been simplified and enhanced with the new automated report generation feature.</li>
<li>See the workflows that are enabled on the AssayMAP platform with new large capacity AssayMAP cartridges.</li>
</ul>
<p>Who Should Attend:</p>
<ul>
<li>Scientists doing biotherapeutic drug development from discovery to pre-clinical work.</li>
<li>People involved in quality control and assurance.  </li>
<li>People looking to increase reproducibility and throughput. </li>
<li>Scientists doing proteomics and phosphoproteomics research.</li>
</ul>
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<p><strong>Presenter:</strong> Steve Murphy, Ph.D. (Workflows and Applications Manager, Agilent Technologies)</p>
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<p><strong>Presenter:</strong> Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)</p>
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C&EN: Automated Protein Sample Prep For Non-Automation Experts

Automated Protein Sample Prep For Non-Automation Experts

The AssayMAP Bravo is a flexible yet easy-to-use automated micro chromatography-based protein sample preparation platform.

<p><strong>Unleash the power of DIA and enable the exploration of complex biological systems with unprecedented depth and precision for large-scale quantitative protein analysis combined with short methods on the Evosep One.</strong></p>
<h3><strong>Global proteomics with Evosep and DIA-PASEF</strong></h3>
<p>Recent progress in liquid chromatography and mass spectrometry, as well as sample preparation and search algorithms for spectra deconvolution and identification, plays an important role in bringing proteomics to the forefront of drug discovery and development. Nanoflow liquid chromatography coupled to tandem mass spectrometry is one of the most essential elements in a proteomics workflow. The four dimensional ion detection achieved with the latest generation of Bruker’s trapped ion mobility spectrometers led to superior speed and sensitivity. Evosep One liquid chromatography platform offers speed and robustness while maintaining, even exceeding previously achieved performance level with high-performing nano LC systems. We share here some results only possible with this combination of LC-MS and dia-PASEF.</p>
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<p><strong>Presenter:</strong> Anh Reynolds (Scientist, Bristol Myers Squibb)</p>
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<h3><strong>Extending sensitivity limits with Evosep and Slice-PASEF</strong></h3>
<p>Sensitivity improvements in LC-MS proteomics have recently led to exciting new applications, such as comprehensive proteomic profiling of single cells or 2-dimensional proteomics of tissue slices. In this talk, I will describe Slice-PASEF, a new acquisition technology for timsTOF instruments, which, in combination with the DIA-NN data analysis software, provides a fold-change signal boost. When paired with the Evosep system, Slice-PASEF allows to achieve high sensitivity in robust high-throughput workflows, paving a way for accessible sensitive proteomics on a large scale.</p>
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<p><strong>Presenter:</strong> Vladim Demichev (Group Leader, Quantitative ProteomicS, Charité – Universitätsmedizin Berlin)</p>
</blockquote>


Pixabay/sethink: Revolutionizing proteomics with Evosep-DIA

Revolutionizing proteomics with Evosep-DIA

Global proteomics with Evosep and DIA-PASEF & Extending sensitivity limits with Evosep and Slice-PASEF.

<h5><strong>The Problem&nbsp;</strong></h5><p>When working with adenovirus (AdV) vectors it’s a big deal to get fast answers on capsid titer, empty/full ratio, and if things are aggregated. UV/Vis absorbance at 260 nm (A260) is considered the quickest check of AdV titer – but it requires denaturing your AdV, easily making it a 30 minute assay. On top of that, A260 results get thrown off by protein, DNA contamination, or just about anything else that absorbs. Other more technical methods to get titer, like qPCR, are too slow, hands-on or sample-hungry, and none are ready for a quick, easy check of your adenovirus sample.</p><h5><strong>The Solution&nbsp;</strong></h5><p>Stunner’s unique combination of UV/Vis with static and dynamic light scattering (SLS &amp; DLS) gives researchers a full read-out on AdV titer, empty/full ratio, and aggregation – in about a minute using only 2 µL.</p><h5><strong>The Proof&nbsp;</strong></h5><p>In this seminar you’ll learn how Stunner delivers dye-free, standard-free, and hassle-free titers down to 1E9 vp/mL, and checks the size and aggregation of 96 samples in just 1 hour. We’ll look at all the info Stunner delivers, how these can be used for adenoviral vector sample QC, and how adenovirus from a bunch of different vendors stacks up.<br>&nbsp;</p><blockquote><p><strong>Presenter:&nbsp;</strong>Nelis Denys (Product Manager, Unchained Labs)</p><p><strong>Moderator: </strong>Kevin Lance PhD<strong> </strong>(Marketing Manager, Analytical Instruments, Unchained Labs)</p></blockquote>

Unchained Labs: Snag spot-on adenovirus titers & empty/full ratios with Stunner

Snag spot-on adenovirus titers & empty/full ratios with Stunner

Join our webinar and check out concentration, labeling, stability, and viscosity data of a mAb and its conjugate. 

LCMS webinars focusing on Sample prep - page 28