Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Metrohm

Mettler-Toledo

Technology Networks

Wiley

C&EN

Noisy Signal? Sloping Baseline? Current Spikes? Electrochemistry can be tough to get right sometimes, so join us for this free-to-attend webinar and learn step-by-step processes on how to troubleshoot everyday problems you might have encountered before in the lab.
<blockquote>
Presenter: Dr. James Taylor (Application Scientist, Metrohm Autolab)
</blockquote>

Metrohm USA: Troubleshooting Electrochemical Systems

Troubleshooting Electrochemical Systems

Learn step-by-step processes on how to troubleshoot everyday problems you might have encountered with electrochemistry

This webinar is offered several times. Select the date and time that works best for you.
<ul>
<li>Tue, Mar 19, 2024, 10:00 AM - 11:00 AM CET</li>
<li>Tue, Mar 19, 2024, 3:30 PM - 4:30 PM CET</li>
</ul>
Ever wondered about what&#39;s inside your potentiostat and whether it really matters? In this webinar we&#39;ll answer questions you&#39;ve always wanted to ask, explain how a potential is applied or a current measured in a conventional three-electrode setup. Do you know what the compliance voltage or the bandwidth of the instrument is, and why one should care?
With a good understanding of how the instrument is made and operates you’ll be able to make sense of the data it provides, troubleshoot common electrochemical experiments, and boost your confidence in the lab. 
This webinar is accessible to anyone: no background in electronics is required. 
<blockquote>
Presenter: Thomas Touzalin, PhD. (Product Manager at Metrohm Autolab)
</blockquote>
<blockquote>
Helping the electrochemistry researchers achieve their goals by providing the right solution for any research problems as well as offering technical support for the Autolab product range.
</blockquote>
Surprise: We will be giving away the &quot;A guide to flawless electrochemical measurements&quot; lab poster, a step-by-step guide to address common issues you may encounter when using a potentiostat. You can hang it in your lab to ensure the success of every experiment. If you would like to stand a chance to win one through a lucky draw, please indicate below.

Metrolab: Beyond the black box: how a potentiostat works

Beyond the black box: how a potentiostat works

In this webinar we'll explain how a potential is applied or a current measured in a conventional three-electrode setup.

Event Program
<ul>
<li>Introduction</li>
<li>Temperature effects on sensors</li>
<li>Temperature effects on buffers/solutions</li>
<li>Temperature compensation on the part of the sensor</li>
<li>Temperature compensation on the part of the buffers</li>
<li>Summary</li>
<li>Q&amp;A and feedback</li>
</ul>
The temperature plays a critical role in pH measurements. Achieving accurate pH values is not only about the chemical composition of a solution but also about understanding the impact of temperature fluctuations. Temperature can significantly influence the behavior of pH electrodes and the characteristics of the sample, making it essential to understand its effects on pH measurements. Challenges arise when temperature differentials between the electrode and the sample lead to unstable readings, highlighting the need for a deeper understanding of temperature&#39;s influence on pH.
During our webinar, you will learn about the relationship between temperature and pH. From the temperature dependence of the dissociation constant of water to the impact of temperature on the activity coefficient and acid constants, we will see the complexities of temperature effects on pH measurements. Understanding these nuances can help you enhance the accuracy and reliability of your pH measurements, ensuring precise results across diverse temperature conditions.
Register now for our upcoming webinar to gain valuable insights into the effect of temperature on pH and deepen your understanding of this relationship. Join us and gain the knowledge to optimize your pH measurements and achieve consistent, reliable results.
<h5>How does temperature affect pH measurements?</h5>
Temperature can significantly impact pH measurements by altering the behavior of pH electrodes and the characteristics of the sample. Temperature changes can cause the pH electrode to drift until the temperature of the electrode and the sample become equal, leading to unstable readings. Additionally, the temperature dependence of the dissociation constant of water and the solution&#39;s activity coefficient and acid constants can cause changes in the H+ concentration and real pH value changes.
<h5>How can I compensate for temperature effects on pH measurements?</h5>
Temperature compensation tables have been determined for standard buffer solutions, which can be programmed into pH meters to ensure the correct pH value is used for the buffer at the temperature at which the calibration is performed. However, it is almost impossible to compensate for the temperature coefficient of every sample solution, making it essential to measure samples at the same temperature to make accurate comparisons.
<h5>What are the best practices for handling pH electrodes in temperature-sensitive environments?</h5>
To ensure accurate pH measurements in temperature-sensitive environments, it is crucial to use the correct pH electrode for the specific sample and give the electrode enough time to equilibrate and to maintain the electrode properly.
When measuring in samples that do not consist of easy-to-measure clear, aqueous solutions, problems of electrical or chemical origin can occur, which should be addressed by following the manufacturer&#39;s instructions for electrode handling and maintenance.
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Presenter: Dr Reena Mascarenhas
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Reena is an electrochemist, working for METTLER TOLEDO, with over five years of experience in training others in various aspects of the field. Her in-depth understanding, technical expertise, and engaging and informative training style make her a prominent speaker for this webinar.
</blockquote>

Mettler-Toledo: Temperature Effects on pH Measurement

Temperature Effects on pH Measurement

A Live Webinar to Understand the Relation between Varying Temperature and pH.

<h5>Event Program</h5>
<ul>
<li>What exactly is data integrity for electronic record keeping and how does it affect process instrumentation for pharmaceutical water systems?</li>
<li>How can a transmitter and software combination help pharmaceutical companies implement data integrity?</li>
</ul>
<h5>Easy Data Integrity Implementation</h5>
In this live webinar, we will discuss what data integrity for electronic record keeping is and how it impacts process instrumentation for pharmaceutical water systems. 
The presenter will also introduce updates and additions to the RecordLOC transmitter and PC software, the METTLER TOLEDO Thornton solution for implementing data integrity for TOC, ozone, and conductivity in pharmaceutical water systems. 
See how RecordLOC helps pharmaceutical companies easily implement data integrity.
Attendees will have the opportunity to ask the presenter your questions about electronic record keeping.
<blockquote>
Presenter: Joel Kenyon (Senior Global Product Manager for Conductivity and Transmitters for METTLER TOLEDO Thornton.)
</blockquote>
<blockquote>
Joel Kenyon is the Senior Global Product Manager for Conductivity and Transmitters for METTLER TOLEDO Thornton. A biologist by training, for the last 15 years Joel has developed and supported analytical instrumentation for academic and industrial applications across a broad range of disciplines.
</blockquote>

Mettler-Toledo: Data Integrity Requirements For Process Instrumentation

Data Integrity Requirements For Process Instrumentation

We will discuss what data integrity for electronic record keeping is and how it impacts process instrumentation for pharmaceutical water systems.

Improving Process Performance
Event Program
<ul>
<li>The value of in-line measurement of quality-relevant analytical parameters</li>
<li>How a new pH sensor improves brewery processes</li>
<li>The importance of precise recognition of phase changes</li>
<li>How to ensure accuracy of dissolved oxygen sensors</li>
</ul>
Greater Confidence in Your Processes.
In-line measurement of quality-related analytics supplement process control with important information. 
In this webinar, we will show how the in-line measurement of relevant parameters helps to drive processes closer to their optimum.
One focus will be on the in-line measurement of pH and we will present a new sensor technology that simplifies the brewing process from mashing to filling. 
The webinar will also shed light on how the combination of color and turbidity measurement with scattered light at 25° and 90° has a positive influence on mashing, fermentation and filtration. And how transmitted light and reflection sensors optimize phase changes and enable product recognition.
Oxygen measurement along the process helps to detect faults in the system, enabling timely actions before out-of-spec oxygen causes lasting damage to your product.
The large number of measuring points in breweries requires a smart concept for the verification and calibration of in-line dissolved oxygen sensors. A solution for these tasks will also be presented.
<blockquote>
Presenter: Holger Schmidt (Mettler Toledo)
</blockquote>
<blockquote>
Holger Schmidt backs up his practical know how as brewer and malster with a brewmaster degree from TUM Weihenstephan. For two decades he has been globally mediating the triangle between Food and Beverage producers, plant and machine builders, and automation companies, with a focus on hygienic sensor technologies.
</blockquote>

Mettler-Toledo: In-line Quality Control in Breweries

In-line Quality Control in Breweries

In-line measurement of relevant parameters helps to drive processes closer to their optimum.

Modern titration offers a simple, yet highly accurate method to ensure the quality and safety of many products we consume daily. While not a complicated technique, there are some subtleties that can ensure your titration results are accurate. Simple electrode care and optimization of basic titration parameters are crucial to helping you obtain results you can trust.
During this webinar, Sagar Irrinki, Quality Control Manager at Coastal Gulf &amp; International, Inc. will share how his laboratory navigates executing these tests on various types of titration applications. and how to overcome challenges with helpful titration tips. Eduardo Simoes, Product Specialist for Titration at Metrohm USA, will provide guidance for electrode selection and best practices for electrode care and maintenance. Don’t miss the live Q&amp;A at the end to get all your questions answered by Sagar and Eduardo. 
<h5>Key Learning Objectives:</h5>
<ul>
<li>Understand potentiometric electrode care and maintenance </li>
<li>Learn how to overcome titration challenges with useful tips</li>
<li>Receive simple guidelines to remove doubt from titration results </li>
<li>Learn how to select the appropriate electrode for your titration</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone who wants to learn more about electrode care and maintenance</li>
<li>People interested in improving the consistency of their titration results</li>
<li>Anyone who needs a refresher on titration best practices</li>
<li>Chemists who want simple tips to improve confidence in titration analysis</li>
</ul>
<blockquote>
Presenter: Eduardo Simoes (Titration Product Specialist, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Sagar Irrinki (Quality Control Manager, Coastal Gulf &amp; International, Inc.)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

Mastering Potentiometric Titration through Effective Electrode Selection and Method Optimization

Simple electrode care and how to overcome challenges with helpful titration tips.

Mastering Potentiometric Titration

Key Analytical Measurements to Increase Yield
Semiconductor FABS are the largest industrial user of pure and ultrapure water (UPW), which are vital to quality and yield in the production of wafers and integrated circuits.
Today&#39;s industry is challenged to produce more accurate measurements and higher-quality UPW, as well as reduce the volume of water and energy used in production; along with maintaining a focus on water reclaim, reuse, recycle.
With demand for semiconductors continuing to grow and rapid advancements in the industry, critical analytical parameters required for the production of UPW for improved process control and knowledge on how to use these measurements for maximum output are key.
Program
<ul>
<li>Total Organic Carbon control to ensure detection of sub-ppb organics and prevent bacterial contamination.
</li>
<li>Resistivity to monitor the ionic UPW quality and its quality in the RCA process and other SEMI tools.
</li>
<li>Dissolved oxygen and its monitoring and control in UPW.
</li>
<li>pH control as a critical parameter to the CMP process and HF, ammonia waste neutralization Recommendations for segregation of reclaim/reuse/recycle streams.
</li>
<li>The Role and Importance of UPW Measurements.
</li>
</ul>
This webinar is by courtesy of Semiconductor Digest.
<blockquote>
Presenter: Jim Cannon (Head of OEM &amp; Markets, METTLER TOLEDO Thornton)
</blockquote>
<blockquote>
After receiving a B.S. in Biology, he continued with graduate degrees in Microbiology and in Biophysics, and an MBA in Marketing and International Management. Mr. Cannon has over 40 years of experience in the management, design and development of ultrapure water treatment and technology. This includes measuring differential conductivity and UV oxidation for the detection of TOC in UPW and the design and commercialization of Electrodeionization modules. Mr. Cannon is currently involved in the standards and regulatory organizations for the Semiconductor, Photovoltaic and Pharmaceutical markets, including the facilities and liquids committee, Reclaim/Reuse/Recycle Task Force and the UPW task Force. He has also been intimately involved in the development and the commercialization of alternative online microbial detection technology.
</blockquote>

Mettler-Toledo: Water Sustainability Trends in MicroE

Water Sustainability Trends in MicroE

Critical UPW measurements: Organic carbon, resistivity, dissolved oxygen, pH control. Stream segregation recommendations.

For years, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has been the go-to method for protein purity analysis. Despite shortcomings in sensitivity, reproducibility, and its semi-quantitative nature, SDS-PAGE is still widely used.
Capillary electrophoresis (CE-SDS) with its superior separation power, accurate quantitation, and ability for automation is quickly replacing SDS-PAGE in laboratories around the world. In addition to the obvious advantages in time and labor, CE-SDS also stands out as a ‘greener’ method over SDS-PAGE. As laboratories worldwide increasingly focus on waste management with various methods, CE-SDS presents clear advantages over SDS-PAGE for protein purity analysis.​
In this webinar, Prof. Hermann Wätzig from the Institute of Medicinal and Pharmaceutical Chemistry, Braunschweig, Germany and Dr. Chris Heger, Director, Applications Science, Bio-Techne will discuss how transitioning from SDS-PAGE to CE-SDS benefits research, both from an academic and industrial perspective. The speakers will also discuss the current state-of-the art instrumentation and method development landscape.
<blockquote>
Presenter: Dr. Hermann Wätzig (Professor, Technische Universität Braunschweig)
</blockquote>
<blockquote>
Presenter: Dr. Chris Heger (Director, Applications Science, Bio-Techne)
</blockquote>

Wiley: Time to Turn the PAGE on SDS-PAGE!

Time to Turn the PAGE on SDS-PAGE!

Transitioning from SDS-PAGE to CE-SDS benefits research, both from an academic and industrial perspective.

The metal-finishing industry faces the ongoing challenge of managing wastewater effectively, especially when treating dissolved metals such as copper and hexavalent chromium. These metals pose significant environmental risks and introduce compliance and financial challenges. Traditional ion exchange and chemical precipitation methods exhibit limitations in selectively targeting specific metals and rely on hazardous chemical handling and secondary waste product treatment. Both methods have limited effectiveness with increased metal concentrations, competing ions&#39; presence, and dissolved solids&#39; overall concentration.
Join James Landon, Chief Technology Officer at ElectraMet, as he introduces an innovative solution to conventional dissolved metal approaches, employing an electrochemical process to efficiently remove or convert dissolved metals. Beyond treatment, both sustainability and operational optimization hinge on real-time data. Learn how online Process Analyzer Technology (PAT) can revolutionize wastewater management, offering precise, real-time monitoring and analysis.
<h5>Key Learning Objectives:</h5>
<ul>
<li>Understand how the innovative electrochemical process overcomes challenges in metal finishing.</li>
<li>Learn how real-time monitoring and analysis can optimize the wastewater treatment process</li>
<li>Learn how integrating electrochemical processes with PAT can transform wastewater into revenue</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Process Engineers looking to optimize wastewater treatment processes or who are looking to implement PAT in their metal finishing lines</li>
<li>Sustainability and Operations Managers tasked with implementing eco-friendly practices within their organizations and overseeing the day-to-day operations of metal finishing lines</li>
<li>Environmental Compliance Officers tasked with ensuring their facilities meet regulatory standards for wastewater</li>
<li>Wastewater Treatment Consultants advising on wastewater management strategies</li>
</ul>
<blockquote>
Presenter: James Landon, PhD (Chief Technology Officer and Co-founder, ElectraMet)
</blockquote>
<blockquote>
Presenter: Brett Lane (Field Applications Specialist, Process Wet Chemistry, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Ann Thayer (Contributing Editor, C&amp;EN Media Group)
</blockquote>

Metrohm: Beyond Ion Exchange: Advancing Wastewater Treatment in Metal Finishing

Beyond Ion Exchange: Advancing Wastewater Treatment in Metal Finishing

Process Analyzer Technology (PAT) can revolutionize wastewater management, offering precise, real-time monitoring and analysis.

Beyond Ion Exchange: Advancing Wastewater Treatment

Harvest time has come for modern biotechnology and biotherapeutics, bringing a cornucopia of products – CRISPR Cas nucleases, therapeutic viruses, cells and engineered tissue constructs.
Along with superior therapeutic efficacy, these products feature unprecedented complexity of biomolecular features and range of scales that define their identity, efficacy and safety.
In this webinar, our expert speaker Dr. Stefano Menegatti from NC State University, will discuss his team’s approach in leveraging affinity technology to introduce new tools for purifying and characterizing biotherapeutics to match the growing demands of clinics and biotech industries worldwide. 
Attend this webinar to:
<ul>
<li>Learn about affinity ligands operating in negative and positive capture mode for the continuous purification of biotherapeutics</li>
<li>Discover first of its kind RNA triggers for the purification of CRIPSR-CAS nucleases via affinity chromatography</li>
<li>Explore the complexity of EIS sensors for one-step quantification of total virus particle and empty capsid quantification</li>
</ul>
<blockquote>
Presenter: Stefano Menegatti, PhD (Associate Professor, Chemical and Biomolecular Engineering, NC State University)
</blockquote>

Technology Networks: Modernizing the Purification Toolbox for Multi-Specific Antibodies, CRISPR and Cell and Gene Therapies

Modernizing the Purification Toolbox for Multi-Specific Antibodies, CRISPR and Cell and Gene Therapies

Leveraging affinity technology to introduce new tools for purifying and characterizing biotherapeutics.

LCMS webinars focusing on Electrochemistry - page 3