<h3>A Dedicated Solution for Oligonucleotide Analysis</h3>Oligonucleotides have become key players in modern pharmaceutical and biological research, particularly in the development of highly targeted and personalized therapies. As increasing numbers of oligonucleotide-based drugs enter clinical trials or receive regulatory approval, analytical laboratories face growing demands for both throughput and data quality. These molecules present unique chromatographic challenges due to their long, highly polar structures and subtle variations in nucleobase sequence or functionalization.<h3>Sepapure oliGO – Designed for Oligonucleotide Separations</h3>Selecting the optimal chromatographic approach for oligonucleotide analysis can be complex. Many laboratories rely on ion-pair reversed-phase (IP-RP) chromatography because of its speed, robustness, and compatibility with mass spectrometry. However, commonly used ion-pair reagents can degrade conventional silica-based stationary phases.<a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/columns-oligonucleotide-columns">Sepapure oliGO</a> addresses this limitation through a hybrid silica matrix combined with specialized surface end-capping, ensuring resistance to aggressive IP-RP reagents. Its functionalized surface enables both strong reversed-phase interactions and controlled ionic contributions, effectively creating a mixed-mode separation mechanism. This design results in improved durability compared with standard mixed-mode columns, making Sepapure oliGO a robust choice for routine oligonucleotide workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg" alt="KNAUER: Figure 1 - Sepapure oliGO columns with laser-etched column information." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg 223w," sizes="100vw" width="1186" height="828"><h3>Method Transfer and Comparative Performance</h3>In collaboration with BianoGMP GmbH, KNAUER’s application team evaluated Sepapure oliGO against a widely used reference column. The study compared oligonucleotides of varying lengths and purities, including a fully thiolated 43-mer DNA. Particular attention was paid to potential interactions between thiolated oligonucleotides and stainless-steel hardware, which have been reported to cause peak tailing.The results demonstrated that direct method transfer between the reference column and Sepapure oliGO was generally feasible without significant adjustment. Chromatographic resolution remained comparable, while retention times were slightly shorter on Sepapure oliGO. Furthermore, the anticipated benefits of expensive bioinert hardware were not confirmed under the tested conditions, suggesting that such effects may primarily influence very fast (U)HPLC separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg" alt="KNAUER: Figure 2: Overlayed chromatogram of crude product of a 43mer thiolate oligonucleotide. Light blue - reference column and dark blue – Sepapure oliGO." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg 245w," sizes="100vw" width="1223" height="453"><h3>Low Backpressure for Reliable Operation</h3>Sepapure oliGO can be applied effectively in both HPLC and (U)HPLC workflows. Its low operating backpressure minimizes risks associated with clogging, excessive system wear, and performance deterioration over time. Typical IP-RP separations using 3 µm particles generated pressures around 200 bar, enabling laboratories without ultra-high-pressure systems to perform high-quality oligonucleotide analysis.<h3>Best Practices for Column Performance</h3>To maintain optimal performance, users should observe recommended operating conditions, including a broad pH range of 1.5–10 and temperatures up to 60 °C. Employing a guard column can prevent coarse synthesis by-products from reaching the analytical column. If pressure increases are observed, backflushing at low flow rates can restore performance.When the system is idle, thorough flushing to remove salts is essential. For storage, the column should be kept in a 1:1 mixture of methanol–water or acetonitrile–water. Gradient transitions are recommended when switching between standard RP eluents and IP-RP reagents. Regeneration procedures developed for RP columns are also suitable for Sepapure oliGO.<h3>Beyond Analytical Separations</h3>For large-scale purification, ion-exchange chromatography (AEX) may offer advantages. Scalable preparative systems are available to support purification workflows, ranging from compact laboratory setups to pilot-scale platforms with flow rates up to 800 mL/min. Bioinert configurations can be integrated where required, allowing laboratories to tailor purification solutions to their specific oligonucleotide applications.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at folmert@knauer.net&nbsp;</li></ul></blockquote>

Oligos Made Easy - Part 8: Sepapure oliGO

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

News from LabRulezLCMS Library - Week 13, 2026

Article

Product

Events

Application

Science and research

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Metrohm

Thermo Fisher Scientific

This webinar presents solutions for advanced electrocatalysis (ECAT) research and applications.
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Presenter: Julia van Drunen, PhD (Area Manager, Metrohm Autolab, Nl)
</blockquote>
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Presenter: Pietro Lopez, PhD (Staff Scientist Argonne National Lab, USA)
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Metrohm: Electrocatalysis research and applications

Electrocatalysis research and applications

 Solutions for advanced electrocatalysis (ECAT) research and applications.

Watch this webinar to learn more about fast, simple, and sensitive electrochemical biosensing using screen-printed electrodes.
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Presenter: Susana Campuzano, PhD (Asst. Professor, Universidad Complutense de Madrid)
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Presenter: Laura Fernández Llano, PhD (Product Manager, Dropsens)
</blockquote>

Metrohm: Biosensing with screen-printed electrodes

Biosensing with screen-printed electrodes

Learn more about fast, simple, and sensitive electrochemical biosensing using screen-printed electrodes.

Watch this webinar to learn more about common non-destructive electrochemical corrosion techniques.
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Presenter: Julia van Drunen, PhD (Area Manager, Metrohm Autolab B.V.)
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Presenter: Luis F. Garfias, PhD (Consultant, Enviromental and Corrosion Testing of Materials, LLC)
</blockquote>

Metrohm: Corrosion Tests: Non-destructive methods

Corrosion Tests: Non-destructive methods

Learn more about common non-destructive electrochemical corrosion techniques.

In this webinar Dr Julia van Drunen introduces electrochemical impedance spectroscopy (EIS) as a valuable tool for the analysis of battery materials, components, and fuel cells.
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Presenter: Julia van Drunen, PhD (Area Sales and Marketing Manager, Metrohm Autolab)
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Metrohm: EIS Data For Battery Materials R&D

EIS Data For Battery Materials R&D

Electrochemical impedance spectroscopy (EIS) as a valuable tool for the analysis of battery materials, components, and fuel cells.

Noisy Signal? Sloping Baseline? Current Spikes? Electrochemistry can be tough to get right sometimes, so join us for this free-to-attend webinar and learn step-by-step processes on how to troubleshoot everyday problems you might have encountered before in the lab.
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Presenter: Dr. James Taylor (Application Scientist, Metrohm Autolab)
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Metrohm USA: Troubleshooting Electrochemical Systems

Troubleshooting Electrochemical Systems

Learn step-by-step processes on how to troubleshoot everyday problems you might have encountered with electrochemistry

This webinar is offered several times. Select the date and time that works best for you.
<ul>
<li>Tue, Mar 19, 2024, 10:00 AM - 11:00 AM CET</li>
<li>Tue, Mar 19, 2024, 3:30 PM - 4:30 PM CET</li>
</ul>
Ever wondered about what&#39;s inside your potentiostat and whether it really matters? In this webinar we&#39;ll answer questions you&#39;ve always wanted to ask, explain how a potential is applied or a current measured in a conventional three-electrode setup. Do you know what the compliance voltage or the bandwidth of the instrument is, and why one should care?
With a good understanding of how the instrument is made and operates you’ll be able to make sense of the data it provides, troubleshoot common electrochemical experiments, and boost your confidence in the lab. 
This webinar is accessible to anyone: no background in electronics is required. 
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Presenter: Thomas Touzalin, PhD. (Product Manager at Metrohm Autolab)
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Helping the electrochemistry researchers achieve their goals by providing the right solution for any research problems as well as offering technical support for the Autolab product range.
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Surprise: We will be giving away the &quot;A guide to flawless electrochemical measurements&quot; lab poster, a step-by-step guide to address common issues you may encounter when using a potentiostat. You can hang it in your lab to ensure the success of every experiment. If you would like to stand a chance to win one through a lucky draw, please indicate below.

Metrolab: Beyond the black box: how a potentiostat works

Beyond the black box: how a potentiostat works

In this webinar we'll explain how a potential is applied or a current measured in a conventional three-electrode setup.

Achieving product consistency for beer, wine, cider, and other adult beverages requires the analysis of multiple parameters using several automated and/or wet chemistry procedures.
Many labs still follow the traditional wet chemical methods like colorimetry, titration, and flow analysis, as well as measurement methods such as pH, conductivity, and ion selective electrodes. Integrated discrete analysis consolidates beverage testing and simultaneously automates the analysis of critical parameters resulting in faster turnaround, reduced human error, lower reagent use, and expanded laboratory analytical capabilities. Better testing means better product to retain and expand your customer base. We will present an overview of the integrated discrete analyzer and important parameters to measure in adult beverages.
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Presenter: Hari Narayanan (Product Marketing Manager, Thermo Fisher Scientific)
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Dr. Hari Narayanan is the Product Marketing Manager at Thermo Fisher Scientific. He has nearly 20 years of experience in managing research activities and applications development. He collaborates with regulatory agencies like ASTM, EPA, EPRI, USP, and AOAC and has been part of several new method developments. He is well-versed in multivariate analysis, sample preparation, titration, ion chromatography, Combustion IC, molecular spectroscopy (FTNIR, FTIR, RAMAN and UV), voltammetry, flash chromatography, particle size analysis, ICP and XRF. He received his B.Sc and M.Sc. in Chemistry from the Madurai Kamaraj University and a Ph.D. in Analytical Chemistry from the University of Madras, India. Before joining Thermo Fisher Scientific, Hari was Director of Strategic Marketing at Metrohm USA.
</blockquote>

Thermo Scientific: Consistent Quality from Consolidated Beverage Testing

Consistent Quality from Consolidated Beverage Testing

Overview of the integrated discrete analyzer and important parameters to measure in adult beverages.

Wet chemical methods are an integral part of modern analytical labs. Many of the regulatory bodies, such as the US EPA, recommend wet chemical methods like colorimetry, titrimetry, and flow analysis, as well as measurement methods like pH, conductivity, and ion selective electrodes for drinking water and wastewater applications.
Why? Because wet chemical methods are proven and modern instrumentation often cannot determine results that many specific wet chemical tests provide. However, these traditional wet chemical methods are tedious, time-consuming, and consume large volumes of samples and reagents.
In this webinar we will provide an overview of US EPA recommended automated wet chemical methods for drinking water and wastewater and discuss several application examples that highlight the benefits of transferring traditional wet chemical methods to the integrated discrete analyzer.
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Presenter: David Glutz (Product Specialist, Thermo Fisher Scientific)
</blockquote>
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David Glutz has been working at Thermo Fisher Scientific as a Product Specialist for 12 years. He has strong working and method development knowledge and is the go-to person for the Discrete Analyzer product line. David supports customer training and application development for discrete analyzers, ion chromatography, and sample preparation at the Center of Excellence. Trained in Applied Chemical Technology from the University of Cincinnati’s OMI College of Applied Science, David has a unique background in the environmental field with more than 20 years of experience.
</blockquote>

Thermo Scientific: Automated Environmental Wet Chemical Analysis

Automated Environmental Wet Chemical Analysis

Overview of US EPA recommended automated wet chemical methods for drinking water and wastewater and discuss several application examples.

Conventional wet chemistry is manual, labor intensive, and involves large sample volumes.
We will show how a discrete analyzer enables simultaneous multi-parameter analysis and discuss how fully automated wet chemistry analysis in microliter-scale provides cost efficiency and complements ion chromatography.
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Presenter: Hari Narayanan (Product Marketing Manager, Discrete Industrial Analyzer, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dr. Hari Narayanan has nearly 20 years of experience in managing research activities and applications development. He collaborates with regulatory agencies and has been part of several new method developments. He is well-versed in multivariate analysis, sample preparation, titration, ion chromatography, molecular spectroscopy (FTNIR, FTIR, RAMAN and UV), and voltammetry. He received his B.Sc. and M.Sc. in Chemistry from the Madurai Kamaraj University and a Ph.D. in Analytical Chemistry from the University of Madras, India.
</blockquote>

Thermo Scientific: Automate Your Wet Chemistry Analysis

Automate Your Wet Chemistry Analysis

How a discrete analyzer enables simultaneous multi-parameter analysis.

Adoption of cloud-based LIMS solutions continues to grow, as laboratories across all industries consider the financial and operational benefits of cloud-based solutions.
For new laboratories implementing a LIMS for the first time, cloud can provide a quick road to go-live with low capital expenditure and minimal resources to run and manage the system. For existing laboratories looking to modernize their LIMS implementation, cloud can help minimize IT costs while providing unmatched scalability. In this session, we’ll explore real-world customer stories of laboratories that selected cloud deployments for their Thermo Scientific™ SampleManager LIMS™ software.
<blockquote>
Presenter: Katie Evans (Senior Product Manager, Digital Science, Thermo Fisher Scientific)
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Katie Evans is a Senior Product Manager for the Digital Science business at Thermo Fisher Scientific and is located in Altrincham, in the Manchester area of the UK. During her 22 years at Thermo Fisher Scientific, Katie has focused on product management and strategy development of Laboratory Information Management Systems (LIMS) software with an emphasis on the needs of laboratories serving regulated markets. Katie received her Master of Science degree from Exeter University, UK.
</blockquote>
<blockquote>
Presenter. Jon Webb (Senior Sales Manager, Digital Science, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Jon Webb has been with Thermo Fisher Scientific since 2014. His career has focused on helping global R&amp;D organizations manage their scientific data effectively and his work experience has provided him a deep understanding of the benefits of deploying enterprise software to the cloud. His work at Thermo Fisher Scientific has focused on implementing enterprise software solutions to support complex processes and business objectives across the Oil &amp; Gas industry.
</blockquote>

Thermo Scientific: Software to Drive Your Science - Why Some Oil & Gas Companies are Looking to the Cloud

Software to Drive Your Science - Why Some Oil & Gas Companies are Looking to the Cloud

Real-world customer stories of laboratories that selected cloud deployments for their Thermo Scientific™ SampleManager LIMS™ software.

LCMS webinars focusing on Electrochemistry - page 4