<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

<h3>We're excited to announce that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Software</a> version 10.1.2 is now available!</h3>This minor update expands Clarity’s portfolio with new control modules.<h4>Newly added Instrument control modules</h4><ul><li>Ecom auxiliary modules</li><li>HTA ICF control modules</li><li>Spark pump</li></ul><h4>Updated Instrument control modules</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/168">Ecom</a> HPLC</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/328">Knauer</a> HPLC</li><li>Spark ICF control modules</li><li>YoungIn Chromass HPLC</li></ul><blockquote>For a complete list of module changes, see the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelog</a>. For more info about exciting new features directly in Clarity, explore what was introduced in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5622">Clarity 10.1 release highlights</a>.Clarity can control 1000+ instruments; the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">list can be previewed here</a>.</blockquote><h4>Supported Instruments in Clarity (DataApex) — Quick Guide</h4>Clarity Chromatography Software supports a wide range of chromatographs and peripherals via digital control (DD) and analog acquisition (A/D) using the Colibrick module.<h5>Gas Chromatographs (GC)</h5>Digital integration over LAN/RS232 (many via ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li><li>Also supported: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (selected models)</li></ul><blockquote>Note: Most Agilent 6890–8890 and Intuvo use LAN – ICF.</blockquote><h5>HPLC Systems</h5>Full system/module control:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 and Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen series</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h5>HPLC Modules (Pumps, Detectors, Ovens, Fraction Collectors)</h5><ul><li>Pumps: Agilent 1100–1290 families, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detectors: VWD/DAD(PDA)/RID/FLD/ELSD across Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom, etc.</li><li>Column Ovens: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Fraction Collectors: Knauer FC 6.1, Teledyne FOXY series, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h5>Autosamplers</h5><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS modules (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40 series</li><li>CTC Analytics PAL, HTA autosamplers, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR AS</li></ul><h5>Other Devices</h5><ul><li>GC×GC modulators (e.g., Charles University GC×GC Modulator)</li><li>Valves &amp; switch modules (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Degassers, flow monitors, managers, interfaces</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF (and others)</li><li>Balances (EA extension): Mettler Toledo, Sartorius</li></ul><h5>Analog Acquisition (Colibrick)</h5>A/D support for legacy or analog-output devices:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus series, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx, etc.</li><li>HPLC Detectors: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD series, many others</li><li>Elemental &amp; Amino Acid Analyzers: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>If you are interested in a DEMO VERSION, contact us!

DataApex Clarity Chromatography software 10.1.2 just released!

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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This presentation will cover the basic operation of metal speciation by the coupling of chromatography technique with ICPMS. This hybrid analytical technique provides lower detection limits in metal containing compound when compared to LCMS techniques. Some of the tips and tricks will also be discussed in this presentation.&nbsp;<blockquote>Presenter: Yan Cheung, PhD (Application Scientist, Agilent Technologies, Inc.)Yan received her Ph.D. in Analytical Chemistry from Indiana University Bloomington. She joined Agilent Technologies as an Application Scientist in the Life Science and Chemical Analysis team in April 2020. Yan has extensive experience in operating and method development in ICP-MS. Yan also has years of experience working in the oil and gas industry.</blockquote>

Agilent Technologies: Overview of Metal Speciation by LC/IC-ICPMS

Overview of Metal Speciation by LC/IC-ICPMS

This presentation will cover the basic operation of metal speciation by the coupling of chromatography technique with ICPMS. 

Monitoring analytical procedure performance is essential for enabling proactive quality control and effective lifecycle management. In this webinar, you’ll gain practical insights from Phil Borman, Ph.D. from GSK, and Trish Meek, Ph.D. from Waters Corporation, on approaches that support these goals.Phil will introduce the newly published USP General Chapter &lt;1221&gt; on Ongoing Procedure Performance Verification (OPPV), explaining how OPPV ensures reliable procedure performance across the lifecycle through proactive monitoring—leading to continuous improvement and reduced risk.Then, Trish will demonstrate how the waters_connect™ Data Intelligence Software transforms laboratory data into actionable insights. She will highlight how integrated dashboards streamline compliance, enhance productivity, and support smarter, faster decision-making.The session will conclude with a live panel discussion. Bring your questions!<h5>Key learning objectives:</h5><ul><li>"Discover USP&lt;1221&gt; and the role of OPPV in the analytical procedure lifecyle</li><li>Learn how to apply risk-based monitoring and performance metrics</li><li>Explore how data intelligence tools enhance compliance and lab efficiency</li></ul><h5>Who should attend:</h5><ul><li>Pharma analytical scientists and lab managers, QA/QC professionals, Informatics and data specialists and anyone involved in lab digital transformation &nbsp;</li></ul><blockquote>Presenter: Phil Borman, Ph.D. (Sr Director, Pharma R&amp;D, GSK)Dr. Phil J. Borman is a fellow of the Royal Society of Chemistry with over 28 years of experience in the pharmaceutical industry. He holds Masters degrees in both Chemistry and Statistics and a Doctorate of Sciences. As Senior Director at GSK, he leads the Quality by Design and Digital Platforms Group supporting small molecules, oligonucleotides, biopharmaceuticals, and vaccines CMC development. A pioneer in adapting QbD principles to analytical procedures, Phil has widely published in Analytical Chemistry. He has also served as Vice-Chair of the USP Measurement and Data Quality Expert Committee and Chair of the Joint Sub-Committee that developed general chapter &lt;1221&gt;.Presenter:&nbsp;Trish Meek (Sr Director, Connected Science, Waters Corporation)Trish Meek is a Sr. Director and Portfolio Owner in the Informatics Business Segment at Waters Corporation. In her current role, she leads the product management, development, cybersecurity and cloud infrastructure for Waters next generation cloud applications. Trish has over 25 years of experience in the laboratory informatics industry.Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Ongoing Procedure Performance Verification

Gain insights on USP <1221>, OPPV, and waters_connect™ software to improve procedure performance, compliance, and lab efficiency.

<h4>SCIEX OS software summer school - part 2</h4>Ready to take your SCIEX OS skills to the next level?Join us for part 2 of our live summer school series and continue building your confidence in SCIEX OS software. These short, 30-minute sessions are designed to fit into your day and give you actionable insights, fast.<ul><li>Practical, focused 30-minute sessions</li><li>Live Q&amp;A with SCIEX experts</li><li>Build confidence using SCIEX OS software</li><li>Designed for busy scientists and lab professionals</li></ul><h5>Agenda:</h5><ul><li>23 July, 11:00 am CET: MS tuning</li><li>6 August, 11:00 am CET:&nbsp;Compound optimization</li><li>20 August, 11:00 am CET: Data acquisition (setting up your method)</li><li>3 September, 11:00 am CET: Data processing (quantitation)</li></ul>

SCIEX: SCIEX OS software summer school – part 2 

SCIEX OS software summer school – part 2: Data acquisition (setting up your method)

Join us for part 2 of our live summer school series and continue building your confidence in SCIEX OS software. 

SCIEX OS software Data acquisition (setting up your method)

<div>Endocannabinoids (eCBs) are endogenous lipid messengers that interact with cannabinoid receptors CB1/CB2 and play a critical role in the central nervous system, often altered in neurological disorders. The low concentration in tissue posts a challenge to ionize and spatially analyze these compounds. Applying a laser-induced post-ionization (MALDI-2) approach improves sensitivity and limit of detection. After optimization, this method was applied to a mouse model of mild traumatic brain injury.Key Learning Objectives:<ul><li>Gain an understanding of the benefits that MALDI Imaging brings to neuropharmacology applications</li><li>Learn applications where MALDI-2 enhances sensitivity</li><li>Understand the technological benefits of MALDI-2</li></ul><h5>Who should attend:</h5><ul><li>Researchers in pharmacology that want to add a spatial dimension to their research</li><li>Investigators that want to expand their technological understanding of MALDI</li></ul><blockquote>Presenter: Eduardo Sommella, Ph.D., Assoicate Professor, Department of Pharmacy, University of Salerno, Naples, ItalyEduardo Sommella is Associate Professor in Medicinal Chemistry at the Department of Pharmacy of the University of Salerno (Italy), and coordinator of the Mass Spectrometry Platform. His work focuses on the development and application of multi-omics-mass spectrometry-based approaches for drug discovery and clinical-diagnostics fields.</blockquote></div>

Bruker: Visualizing the Spatial Landscape of the Brain Endocannabinoidome by MALDI-2 Imaging 

Visualizing the Spatial Landscape of the Brain Endocannabinoidome by MALDI-2 Imaging 

Explore how MALDI-2 enhances endocannabinoid imaging in brain tissue. Learn how it boosts sensitivity for neuropharmacology and brain injury research.

Visualizing the Spatial Landscape by MALDI-2 Imaging 

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Join us for an insightful webinar on MS Data Processing using Thermo Scientific Chromeleon 7.3.2 software. This session is designed for anyone interested in running mass spectrometry (MS) with Chromeleon software. Whether you are a beginner or an experienced user, this webinar will provide valuable insights and practical knowledge to enhance your MS data processing skills.<h5>Topics:</h5><ul><li>QQQ MS Data Processing: Learn the best practices and techniques for processing triple quadrupole (QQQ) MS data using Chromeleon 7.3.2.</li><li>High-Resolution MS Data Processing: Discover how to efficiently process high-resolution MS data, ensuring accuracy and reliability in your results.</li><li>Chromeleon reporting for MS Users: Get tips and tricks on generating comprehensive reports tailored for MS users, utilizing the powerful reporting capabilities of Chromeleon software.</li></ul><h5>Who Should Attend:&nbsp;</h5>This webinar is open to anyone with an interest in running MS with Chromeleon. Whether you are a researcher, lab technician, or scientist, this session will equip you with the knowledge and tools to optimize your MS data processing workflows.Cannot attend the broadcast on August 21? Please register anyway to automatically receive the link to the recording.<blockquote>Presenter: Sebastian Lopez (Lab Application Specialist | LC &amp; LCMS | Pharma &amp; Biopharma, Thermo Fisher Scientific)Seb has been an HPLC and Mass Spectrometry Specialist at Thermo Fisher Scientific for 5 years and has over 15 years of analytical chemistry experience. He is passionate about enabling customer to achieve their analytical goals especially using technology from Thermo Fisher.</blockquote></div></div></div>

Thermo Fisher Scientific: How to process MS data in Chromeleon CDS software

How to process MS data in Chromeleon CDS software

Join us for an insightful webinar on MS Data Processing using Thermo Scientific Chromeleon 7.3.2 software. 

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Join us for an insightful webinar where we take a deep dive into the integration capabilities of Chromeleon Chromatography Data System (CDS) with other software. This session will provide a comprehensive overview of how Chromeleon CDS interfaces with various third-party applications, enhancing your laboratory's efficiency and data management processes.&nbsp;Whether you aim to streamline your workflows or improve data interoperability, this webinar will provide you with the knowledge and tools to achieve seamless software integration.<h5>Key takeaways:</h5><ul><li>Understand the integration capabilities of Chromeleon CDS with other software</li><li>Learn how to set up and configure interfaces for optimal performance</li><li>Discover best practices for ensuring data integrity and consistency</li><li>Get answers to your specific questions during the live Q&amp;A session</li></ul><h5>Who should attend:</h5><ul><li>Chromeleon CDS users looking to enhance software integration</li><li>IT professionals and system administrators managing laboratory software</li><li>Laboratory managers and analysts seeking to improve data workflows</li><li>Anyone interested in learning more about Chromeleon CDS integration capabilities</li></ul>Don't miss this opportunity to enhance your understanding of Chromeleon CDS and its integration with other software. Register now to secure your spot! &nbsp;<blockquote>Presenter: Kimy Young (Senior Manager, Software Product Specialists, Thermo Fisher Scientific)Kimy Young is the Senior Manager of software product specialists at Thermo Fisher Scientific. She has been with Thermo Fisher since November 2015. Her background includes administration of Chromeleon CDS, laboratory information management systems (LIMS), Quality Assurance (QA), and IT projects.Presenter: Juan Carmona (Chromeleon CDS Specialist, Thermo Fisher Scientific)Juan Carmona is a Chromeleon Specialist at Thermo Fisher Scientific based out of Pflugerville, TX. He has been with Thermo Fisher Scientific for over 15 years. At Thermo Fisher Scientific, he is currently focusing on expanding the customer base using Chromeleon CDS for small networks and enterprise deployments. Prior to joining this group, he was part of the GC/GCMS Center of Excellence team providing support for the product line.Presenter: Monica Driscoll (Regional Marketing Specialist, Thermo Fisher Scientific)</blockquote></div></div></div>

Thermo Fisher Scientific: Chromeleon CDS webinar series

Chromeleon CDS connections – Exploring options for interfaces

Discover Chromeleon CDS integration tips and best practices to improve lab data workflows and software interoperability. Live Q&A with Thermo Fisher experts.

Chromeleon CDS – Exploring options for interfaces

When analyzers underperform, the issue is rarely the instrument itself, it’s the sample. In fact, studies show that up to 80% of analyzer problems originate in the sampling system. If the sample isn’t representative, even the most advanced analyzer can’t deliver meaningful data.This webinar takes a critical look at the role of sampling system design in achieving accurate, timely measurements. You’ll gain insights into how to minimize lag time, avoid dead volume and maintain steady flow, ensuring your analyzer sees the process as it truly is. We’ll also cover practical piping considerations, filtration strategies and loop configurations that preserve sample integrity and improve system responsiveness.Bring your curiosity, challenges and questions, you’ll have the chance to ask them directly to our experts during a live Q&amp;A. This session will feature a live discussion between two industry experts as they walk through real-world examples, highlight common design pitfalls, and unpack the planning decisions that lead to more reliable results.<h5>This webinar is ideal for:</h5><ul><li>Production and Operations Leaders – including Production Managers, Plant Managers, and Operational Managers</li><li>Technical and Quality Experts – such as Process Engineers, Quality Control Specialists, and Automation &amp; Control Engineers</li><li>Strategic Decision-Makers – such as Project Leads and Technical Stakeholders</li></ul><h5>Key learning points</h5><ul><li>Implementation Strategy: Learn how to assess feasibility, avoid common pitfalls, and implement robust sampling.</li><li>System Design &amp; Integration: Explore how to design and build a robust sample flow path.</li><li>Smart Control: Understand how to program QC checks and sequencing logic to boost confidence and reduce errors.</li></ul><blockquote>Presenter: Brad Herbert (Sr. Process Engineer, Jacobs)Brad Herbert is a Sr. Process Engineer with Jacobs. Brad’s areas of focus include ultrapure water, wastewater, chemicals, mentoring, and staff development. Over the past 30 years, Brad has worked as a global facilities engineer, site facilities engineer, facilities manager, design consultant, project manager, and product engineer in the semiconductor and water treatment industries. He has led engineering efforts, managed projects, and supported operations throughout The Americas, Europe, and Asia. His work history includes onsemi, LSI Logic, AMD, IDC (CH2M), DeNora Water Technologies, Monsanto, and Sigma-Aldrich. Brad is a licensed Professional Engineer and has a B.S. Degree in Chemical Engineering from the University of Missouri.Presenter:&nbsp;Brett Lane (Process Wet Chemistry Field Applications Specialist, Metrohm USA)Brett joined Metrohm USA in 2021 after working as a senior chemist in an industrial/pharmaceutical quality control laboratory. He has a background in auto-titration, chromatography, mass spectrometry and spectroscopy. His current focus is on applications development for advanced online wet chemistry analysis including titration and ion chromatography systems. Brett graduated from the University of Guelph in 2016 with a BSc. in Chemical Physics, spending time at several R&amp;D internships.</blockquote>

Metrohm: What Your Analyzer Isn’t Telling You: The Role of Sampling Design

What Your Analyzer Isn’t Telling You: The Role of Sampling Design

Learn why sampling—not the analyzer—is often the weak link. Improve accuracy with better system design. Join industry experts for insights and real-world tips.

What Your Analyzer Misses: Sampling Design

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>This presentation explores the use of Enhanced Dynamic Range (EDR) mass spectrometry (MS) to investigate metabolic communication in hepatocellular carcinoma (HCC). EDR MS significantly improves the detection and quantification of metabolites by enhancing the signal-to-noise ratio and peak shapes, making it possible to identify low-abundance metabolites that were previously undetectable. This advancement provides deeper insights into crucial metabolic pathways, such as the valine pathway, cholesterol metabolism, and folate metabolism, which are vital for cancer cell growth, proliferation, metastasis, and drug resistance.&nbsp;The study underscores the importance of understanding metabolic rewiring in HCC to identify novel drug targets. Utilizing advanced MS equipment like the Thermo Scientific Orbitrap Excedion Pro, researchers can achieve a more comprehensive view of cancer metabolism. The findings highlight the potential of EDR MS to significantly enhance cancer research, offering new avenues for therapeutic strategies in treating HCC.&nbsp;<h5>Learning points:</h5><ul><li>Enhanced Dynamic Range (EDR) mass spectrometry (MS) improves detection of low-abundance metabolites, providing deeper insights into metabolic pathways in hepatocellular carcinoma (HCC).&nbsp;</li><li>Understanding metabolic rewiring in HCC through EDR MS can identify novel drug targets and aid in developing new therapeutic strategies.&nbsp;</li><li>Advanced MS equipment, like the Thermo Orbitrap Excedion Pro, enhances the detection and quantification of metabolites, offering a comprehensive view of cancer metabolism.</li></ul><h5>Who should attend:</h5><ul><li>Cancer researchers and oncologists interested in metabolic pathways and novel therapeutic targets in hepatocellular carcinoma (HCC).&nbsp;</li><li>Biochemists and molecular biologists focusing on advanced mass spectrometry techniques and their applications in cancer research.&nbsp;</li><li>Pharmaceutical scientists and drug developers looking for new insights into cancer metabolism and potential drug targets.&nbsp;</li></ul>If you cannot attend this webinar please register to receive a link to the On Demand version the following day. &nbsp;<blockquote>Presenter: Prof. dr. C.R. Celia Berkers (Professor, Universiteit Utrecht)Celia studied chemistry at Utrecht University, where she graduated cum laude in 2003. She started her PhD at Harvard Medical School in Boston and continued her research at the Netherlands Cancer Institute in Amsterdam, obtaining her PhD cum laude in 2010. Since 2013 Celia is a research group leader at Utrecht University’s Bijvoet Center for Biomolecular Research.She received a Rubicon Fellowship from the Netherlands Organisation for Scientific Research (NWO) in 2011. In 2013, that same organisation awarded her a Veni Grant. In 2014 she received a Young Scientists Award for Biochemistry and Biophysics for her research into the workings of the proteasome, a structure that breaks down proteins in biological cells.​</blockquote></div></div></div>

Thermo Fisher Scientific: Enhanced Dynamic Range MS Reveals Metabolic Communication in HCC

Enhanced Dynamic Range MS Reveals Metabolic Communication in HCC

EDR MS boosts detection of low-abundance metabolites in HCC, offering deeper metabolic insights and guiding new therapeutic strategies.

EDR MS Reveals Metabolic Communication in HCC

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>The presentation outlines the installation and utilization of the Orbitrap Astral mass spectrometer for multiomics analysis. Bart highlights the central dogma of biology, emphasizing the importance of integrating proteomics, metabolomics, and lipidomics to gain a comprehensive understanding of biochemical processes. The feasibility study involved analyzing various mouse tissues, demonstrating significant protein and metabolite identification. The presentation addresses challenges in multiomics, including sample preparation, methodology, and data processing.&nbsp;The speaker showcases the advantages of fractionation and the use of advanced mass spectrometry techniques to achieve high-quality data. The presentation concludes with acknowledgments to team members and funding institutions, emphasizing the collaborative effort in advancing multiomics research.&nbsp;<h5>Learning points:</h5><ul><li>Comprehensive Multiomics Approach: Emphasizes the importance of integrating proteomics, metabolomics, and lipidomics to gain a holistic understanding of biochemical processes and disease models.&nbsp;</li><li>Challenges in Multiomics Analysis: Highlights the difficulties in sample preparation, methodology selection, and data processing, and suggests fractionation as a solution to isolate different levels of metabolites, proteins, and lipids.&nbsp;</li><li>Technological Advancements: Showcases the use of advanced mass spectrometry techniques, such as the Orbitrap Astral, for fast discovery and slow validation in multiomics research, ensuring robust and reproducible data.&nbsp;</li></ul><h5>Who should attend:</h5><ul><li>Researchers in Biochemistry and Molecular Biology: Individuals interested in understanding the integration of proteomics, metabolomics, and lipidomics.&nbsp;</li><li>Mass Spectrometry Specialists: Professionals looking to learn about the latest advancements and applications of the Orbitrap Astral mass spectrometer.&nbsp;</li><li>Multiomics Data Analysts: Experts focused on overcoming challenges in data processing and methodology in multiomics research.</li></ul>If you cannot attend this webinar please register to receive a link to the On Demand version the following day. &nbsp;<blockquote>Presenter: Prof. Bart Ghesquière (Expert technologist, VIB - KU Leuven)Bart Ghesquière is an expert technologist leading the VIB – KU Leuven Metabolomics Expertise Center since 2014. Trained as a biotechnologist at the University of Ghent, he worked on chromatographic technologies and mass spectrometry for studying protein modifications and N-glycosylation. He joined NIH in 2011 to quantify methionine oxidation in biological models, earning the Orloff Science award. In 2012, he applied his mass spectrometry expertise to angiogenesis research under Prof Peter Carmeliet.Currently, he leads a team of 11 researchers, focusing on tracer metabolomics to profile metabolic disorders and develop technology. Bart Ghesquière is a Full Professor at KU Leuven and head of the Metabolomics Expertise Center at VIB.</blockquote></div></div></div>

Thermo Fisher Scientific: Mining the multi-ome

Mining the multi-ome

Orbitrap Astral powers multiomics by integrating proteomics, metabolomics, and lipidomics, overcoming key challenges for robust, high-quality data.

<h4>Nitrosamine Analysis Made Simple: Achieve Compliance with Shimadzu Mass Spectrometry</h4>Nitrosamine impurities are a growing concern in the pharmaceutical industry due to their toxicity and strict regulatory limits. This webinar will showcase how Shimadzu’s high-sensitivity LC-MS and GC-MS platforms help streamline nitrosamine detection in complex matrices, reduce method development time, and ensure compliance with FDA, EMA, and ICH M7 guidelines. Through expert insights and real-world examples, you’ll learn how to implement efficient workflows for your QA/QC and R&amp;D labs.<h5>Learning Objectives:</h5><ul><li>An overview of current regulatory requirements for nitrosamine testing (USP and FDA)</li><li>Challenges in detecting nitrosamines in complex pharmaceutical matrices.</li><li>How Shimadzu’s LC-MS and GC-MS systems deliver high sensitivity and reliable results.</li><li>Streamlined workflows techniques to reduce analysis time.</li><li>Real-world case studies demonstrating successful nitrosamine analysis in QA/QC and R&amp;D.</li></ul><h5>Who should attend:</h5><ul><li>Pharma QA/QC</li><li>Pharma R&amp;D</li><li>Regulatory Affair in need to comply with nitrosamines analysis</li></ul><blockquote>Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin is the Market Manager for Pharma/BioPharma at Shimadzu Scientific Instruments. Prior to that, Kevin worked in Analytical R&amp;D in biopharma for over 6 years, with experience in characterization for biologics. He also has over 3 years of experience in the academia field doing neuroscience research.</blockquote>

Shimadzu: Are you Nitrosamine Ready? Solutions for Nitrosamine Analysis

Are you Nitrosamine Ready? Solutions for Nitrosamine Analysis

Shimadzu webinar: Simplify nitrosamine testing with LC-MS/GC-MS. Achieve regulatory compliance and streamline workflows for pharma QA/QC and R&D labs.

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