<p><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></p><h4><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h4><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h4><strong>Key takeaways</strong></h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h4><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h4><strong>Online SPE analysis provides key benefits like:</strong></h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h4><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h4><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h4><strong>The process for analyzing a hops standard solution is straightforward:</strong></h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h4><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h4><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4><strong>From carefully procured samples to tasty sips</strong></h4><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h4><strong>FAQs</strong></h4><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

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KNAUER

<p><strong>Streamlining workflows for higher productivity, sample purity and recovery are primary goals for any laboratory working within the field of preparative liquid chromatography. However, the path to success can vary widely. In this exclusive webinar series, produced in partnership with Agilent Technologies, experts from three different application areas will share their experiences and best practices to help you overcome the key challenges and setbacks associated with preparative  HPLC.</strong></p>
<p>Webinar 1 of Best practices to overcome challenges in preparative HPLC webinar series (November 03, November 10, &amp; November 24)</p>
<h4><strong>Keeping up with the world of purification in early drug development</strong></h4>
<p>In recent years, the demand for outsourced purification services has grown exponentially. Almost every Contract Research Organization (CRO) in Europe now employs a purification team to support its chemists. Join Peter Ridgway, co-founder and director of Reach Separations, for an overview of how purification in the field of early drug development has evolved. Ridgway will share insights from the challenging world of contract purification and discuss some of his more complex purification projects.</p>
<blockquote>
<p><strong>Presenter: Stéphane Dubant (Principle Scientist, Reach Separations Europe)</strong></p>
</blockquote>
<blockquote>
<p>Stéphane’s background is in organic synthetic chemistry, but he quickly became interested in chromatography. Initially he was involved in purification (which is still his favorite subject), and later in analytical chromatography. Stéphane has a broad industrial background. During his 5 years at Pfizer (UK), he delivered purification methods and tested and implemented new technologies such as CCC or SFC for purification purposes. He then moved to Waters as a purification and supercritical fluid specialist, where he had the opportunity to extend his experience to other application areas such as the petrochemical, food or cosmetics industries throughout Europe. He then joined Agilent as a liquid phase separation specialist in France. Currently, Stéphane is Principal Scientist at Reach Separations Europe and will be helping to set up the new Reach facilities in Strasbourg in early 2022.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Carrie Haslam (Associate Editor, SelectScience)</strong></p>
</blockquote>
<blockquote>
<p>Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Alzheimer’s disease and clinical diagnostics. She is finalizing her Ph.D. from Plymouth University, UK, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.</p>
</blockquote>


SelectScience: Best practices to overcome challenges in preparative HPLC: webinar series

Keeping up with the world of purification in early drug development

<p><strong>Fast, confident answers to everyday research questions are essential to modern drug discovery. In purification labs, confident answers often come at the expense of speed.</strong></p>
<p>This webinar will present strategies to maximize resolution while increasing sample throughput and demonstrate how drug discovery labs can capitalize on superficially porous particles in preparative LC columns to drive speed and efficiency.</p>
<blockquote>
<p><strong>Presenter: Lakshmi Subbarao (Applications Scientist, Preparative HPLC Columns, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Lakshmi Subbarao has over 15 years of experience in the chromatography industry, including analysis and purification and pharmaceutical and natural products. Her work has been published in many industry magazines and she has provided training and consultation services to Fortune 100 Pharmaceutical Companies. She received a Bachelor of Science Degree in Chemistry from the University of Delaware and a Master&#39;s Degree in Analytical Chemistry from Illinois Insitute of Technology. Lakshmi is currently a Senior Application Chemist at Agilent Technologies, supporting small-molecule preparative LC columns.</p>
</blockquote>


Agilent Technologies: Scale Up to the Drug Discovery Fast Lane with Superficially Porous Particle Preparative LC Columns

Scale Up to the Drug Discovery Fast Lane with Superficially Porous Particle Preparative LC Columns

<p><strong>As a synthetic chemist, biologist or engineer, it’s your job to study the impact of chemical compounds. In situations where the compound of interest is not available in pure form, you are challenged to purify it yourself. Possible scenarios include synthesis of the compound in multiple stages, using purification as an interim step, or isolation of the compound from a natural source when synthesis is too complex and tedious. Other scenarios that require you to turn to purification techniques include, for example, when flash chromatography did not yield the desired purity, or when crystallization did not work the way you expected.</strong></p>
<p>Do you want to overcome these challenges? Yes.... Join us for next ChromTalk Episode, where we shall discuss in details about the fundamentals behind purifications, selection of parameters to get the best yield, choice of columns &amp; system solutions &amp; automation for throughput requirements.</p>


Agilent Technologies: ChromTalk Episode 10: Concern for Compound Isolation or Purification? 

ChromTalk Episode 10: Concern for Compound Isolation or Purification? 

<p><strong>At the analytical scale, liquid chromatography (LC) is widely used by scientists to separate, quantify, and characterize components in mixtures. At the preparative scale, LC is primarily used to purify samples for further downstream analysis. The adoption of preparative LC into a workflow, with larger columns and instrumentation, can appear to be a daunting task. However, by following some fundamental guidelines and a little practical advice, it can be quite simple.</strong></p>
<p>This webinar will outline the purification process and the steps for a successful purification. We will also discuss the considerations when establishing a purification laboratory, and how to identify and avoid common pitfalls during purification.</p>
<h3><strong>Key learning objectives</strong></h3>
<ul>
<li>Become familiar with the analytical to preparative chromatography workflow</li>
<li>Learn strategies to efficiently develop methods for purification success</li>
<li>Know how to avoid or recover from common mistakes</li>
<li>Understand how to think like a purification scientist</li>
</ul>
<h3><strong>Who should attend?</strong></h3>
<ul>
<li>Analytical and medicinal chemists who want to learn more about purification or preparative LC, or regarding scale-up from analytical to preparative LC</li>
<li>Lab managers supporting purification labs</li>
<li>Scientists who are curious about incorporating purification into their laboratory workflow</li>
</ul>
<blockquote>
<p><strong>Presenter: Lakshmi Subbarao (Applications Scientist, Preparative HPLC Columns, Agilent)</strong></p>
</blockquote>


SelectScience: Level up your scale-up: Practical advice for scaling from analytical to preparative liquid chromatography

Level up your scale-up: Practical advice for scaling from analytical to preparative liquid chromatography

This webinar will outline the purification process and the steps for a successful purification.

Advices for scaling from analytical to preparative HPLC

<p><strong>Activated carbons are widely used for purification and separation applications. However, they are not employable as chromatography media due to the lack of chemical purity, irregular shape, poor mechanical integrity, and lack of reproducibility.</strong></p>
<p>Carboxen® synthetic carbon adsorbents are a synthetic form of activated carbon. These synthetic carbons are derivatized resins. Due to their high purity, spherical shape, and outstanding mechanical properties, Carboxen® synthetic carbon adsorbents are well suited for use in low-pressure liquid chromatography (LPLC) applications. This chromatography media can withstand pressures up to 110 MPa, which is several orders of magnitude greater than other LPLC media. The tapered pores improve the adsorbent’s kinetic and thermodynamic efficiency, enabling greater capacity while operating at increased throughputs. The highly engineered, derivatized resins bring value in both adsorption and hydrophobic interaction chromatography.</p>
<p>These media bring value to a wide range of LPLC applications for both small and large molecule separations. As example, these adsorbents are used for clearance of high-risk impurities in biochemical purifications such as host cell proteins, trace metals, and extractables and leachables. These synthetic carbons have been trusted by NASA on several missions including their use on the International Space Station.</p>
<h5><strong>By attending this presentation you will...</strong></h5>
<ul>
<li>learn about the features, advantages, and benefits of synthetic carbon separation media</li>
<li>understand how a carbon media derived from resin offers a unique stationary phase</li>
<li>discover several applications in both liquid and gas purifications where synthetic carbons bring value.</li>
</ul>
<blockquote>
<p><strong>Presenter: Joe Abrahamson (Global Product Manager, Carbon Adsorbents and LPLC, MilliporeSigma)</strong></p>
</blockquote>
<blockquote>
<p>Dr. Joe Abrahamson received his Ph.D. from Penn State, where he studied carbon materials and engineering. Before joining MilliporeSigma, Joe spent several years in the carbon and materials industries as an R&amp;D scientist. Presently, Joe is the Global Product Manager for Carbon Adsorbents and LPLC at MilliporeSigma. Joe enjoys splitting his time between product management and product development.</p>
</blockquote>


Separation Science: Carboxen® Synthetic Carbon: A Derivatized Resin for Process and Preparative Chromatography

Carboxen® Synthetic Carbon: A Derivatized Resin for Process and Preparative Chromatography

Webinar highlighting the advantages and value of synthetic carbon separation media in both liquid and gas purifications.

Carboxen Synthetic Carbon: A Resin for Chromatography

<p><strong>Agilent proven workflows designed for biopharma and optimized to meet the challenges of oligonucleotide assessment can help with the productivity needs of your lab.</strong></p>
<p>We know that time taken away from the lab is often limited, so we are pleased to present our half day <strong>Oligos Virtual Event</strong> with three short talks, each followed by a Q&amp;A with a Biopharma specialist.</p>
<p>Register to the event on <strong>28 September</strong> to listen to the latest Biopharma innovations Agilent has to offer, and additionally you can request a <strong>software consultation</strong> on a day convenient to you.</p>
<h3><strong>Agenda</strong></h3>
<p>Join us for a morning of short presentations focusing on oligonucleotide analysis. Our experts will cover a range of topics including LC method development; technique comparisons; and software introduction. Each presentation will be followed by a Q&amp;A session with our technical specialist.</p>
<h5><strong>10:30-11:00 Tips &amp; Tricks for Oligonucleotide Analysis</strong></h5>
<ul>
<li><p>Scaling purification from analytical to prep LC</p>
</li>
<li><p>From your sample to the data: Improving your HPLC method (RP and IEX)</p>
</li>
</ul>
<h5><strong>11:00-11:30 Alternative Analysis Techniques for Oligonucleotides</strong></h5>
<ul>
<li><p>Capillary Electrophoresis (CE) and CE/MS</p>
</li>
<li><p>Advantages and limitations comparing CE &amp; LC</p>
</li>
</ul>
<h5><strong>11:30-12:25 In-depth Analysis of Oligonucleotides</strong></h5>
<ul>
<li>LC/MS advantages with new data analysis tools (Mass Hunter Bioconfirm 12)</li>
</ul>


Oligos Virtual Event

Innovations in Oligonucleotide Analysis. Lab productivity is at the forefront of every Biopharma lab.

<p><strong>This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.</strong></p>
<p>Learning Objectives:</p>
<ul>
<li><p><strong>Differences between Semi-micro and Analytical Scale Prep</strong></p>
</li>
<li><p><strong>Things to consider in semi-micro and analytical scale purification</strong></p>
</li>
<li><p><strong>Instrumentation and software advantages</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Earl Mckoy (Senior LC/LCMS Technical Specialist, Shimadzu UK)</p>
</blockquote>


Shimadzu: Semi-micro to Analytical Scale LC Purification

Semi-micro to Analytical Scale LC Purification

Differences between Semi-micro and Analytical Scale Prep.

<p><strong>This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.</strong></p>
<p>Learning Objectives:</p>
<ul>
<li><p><strong>Principles, common issues and tips and tricks of Semi-Preparative Chromatography</strong></p>
</li>
<li><p><strong>UFPLC - Ultra Fast Preparative and Purification LC</strong></p>
</li>
<li><p><strong>Semi-preparative SFC Scale Purification</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Earl Mckoy (Senior LC/LCMS Technical Specialist, Shimadzu UK)</p>
</blockquote>


Shimadzu: Semi-Preparative Scale LC Purification

Semi-Preparative Scale LC Purification

Principles, common issues and tips and tricks of Semi-Preparative Chromatography.

<p><strong>Purify an Analyze using Cannabis as an example</strong></p><p>Using cannabis as an example, we will present our new systems *Cannabis Profiler, *Cannabis Purifier and *Cannabis Producer and will be happy to answer your questions. Of course we are also looking forward to great virtual conversations, interesting "encounters" and informative exchange.</p><blockquote><p><strong>Presenter: </strong>Lisa Loxterkamp</p><p><strong>Presenter: </strong>Max von Delbrück</p><p><strong>Presenter: </strong>Paul Pietsch</p></blockquote>

Purify and Analyze Cannabis

Using cannabis as an example, we will present our new systems Cannabis Profiler, Cannabis Purifier and Cannabis Produce.

<p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}"><strong>Anson Lewis, Global Sales Manager at Tosoh Bioscience, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. On April 8, Marissa Heino, Product Manager Process Solutions, and Abbie Breton, Application Development Manager at Tosoh Bioscience, will demonstrate the advantages of multi-column chromatography (MCC) technology.&nbsp;</strong></span></p><p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}">Batch purification leads to a substantial rise in expenses for end users, including costs of resin, buffers, and labor due to extended processing times. Advances in downstream mAb processing, such as multi-column chromatography systems, have proven effective in significantly lowering purification costs while increasing productivity. In this study, Marissa and Abbie highlight the advantages of MCC technology by showcasing a two-step purification platform using a benchtop MCC system to reduce adventitious viruses, with seamless scalability to production-scale MCC systems.&nbsp;</span></p><blockquote><p><strong>Presenter:</strong> Anson Lewis (Global Sales Manager at Tosoh Bioscience)</p><p><strong>Presenter: </strong>Marissa Heino (Product Manager Process Solutions)</p><p><strong>Presenter:&nbsp;</strong>Abbie Breton (Application Development Manager at Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Intensified Batch Chromatography: A Viral Clearance Case Study

Tosoh Tuesday - Intensified Batch Chromatography: A Viral Clearance Case Study

Marissa Heino and Abbie Breton will demonstrate the advantages of multi-column chromatography (MCC) technology.

LCMS webinars focusing on PrepLC - page 5