Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Metrohm

The Analytical Scientist

Thermo Fisher Scientific

C&EN

Shimadzu Corporation

We live in a world of routine, familiarity and comfort. Anything outside of that is scary. It’s not often that we step outside of our comfort zones. Let’s be curious and take a step to the wild side of chromatography with the familiarity that we seek such as HPLC and GC, where we find out more than just counterions. Exploring the world of impurities whether they are organics or metal species, with simple changes and utilizing different detection can help solve many analytical challenges.
Who should attend?
Pharma, Chromatography, MS customers, Lab scientists and Lab managers
What you will learn:
<ul>
<li>The capability of ion chromatography to help solve analytical challenges</li>
<li>How to apply ion chromatography at different stages of drug development</li>
<li>Preventative and troubleshooting drug development with ion chromatography for impurities</li>
</ul>
<blockquote>
Presenter: Wai-Chi Man (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Wai-Chi has specialized in ion chromatography (IC) since 1989, with over 36 years of experience in analytical chemistry in a wide variety of techniques and industries. Her work at Thermo Fisher Scientific has supported a wide range of customers from various industries, providing IC support in method development, troubleshooting, sample preparation, training, seminars, events, and workshops.
</blockquote>

Thermo Fisher Scientific: How ion chromatography plays a vital role in drug development

How ion chromatography plays a vital role in drug development

How to apply ion chromatography at different stages of drug development. Preventative and troubleshooting drug development.

How ion chromatography plays a role in drug development

EPA Draft Method 1621 represents a shift in thinking in how we screen for PFAS and other fluorinated compounds in the environment, giving a better picture of the full scope of contamination. The method uses combustion ion chromatography (CIC) to determine adsorbable organic fluorine (AOF) in water samples, a non-targeted sum parameter defined by the method itself.
In this webinar, Amelia Paolantonio, from Enthalpy Analytical, will show attendees how she was able to successfully implement this method in her lab. Webinar participants will gain valuable insights into best practices for the method and things to watch out for, such as the importance of cleanliness. Dr. Jay Gandhi, from Metrohm USA, will discuss how to execute a total solution for your fluorine analysis to help you comply with Draft Method 1621. 
Key Learning Objectives:
<ul>
<li>Learn about EPA Draft Method 1621 and its requirements
</li>
<li>Find out the challenges of executing this method and the strategies to overcome them
</li>
<li>Learn how to implement a total solution for complying with the method
</li>
</ul>
Who Should Attend:
<ul>
<li>Environmental lab technicians, analysts, lab managers, and lab directors
</li>
<li>Those interested in learning about new monitoring technologies for PFAS and fluorinated compounds
</li>
</ul>
<blockquote>
Presenter: Amelia Paolantonio (Senior Scientist, Enthalpy Analytical)
</blockquote>
<blockquote>
Presenter: Dr. Jay Gandhi (Vertical Markets Manager, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)
</blockquote>

C&EN: The Changing PFAS Landscape: Insights Into EPA Draft Method 1621

The Changing PFAS Landscape: Insights Into EPA Draft Method 1621

EPA Draft Method 1621 represents a shift in thinking in how we screen for PFAS and other fluorinated compounds in the environment

Insights Into EPA Draft Method 1621

Discrete analyzers are robust, easy-to-use, and provide parallel measurement of multiple analytes in as fast as 10 minutes. Compared to traditional wet chemistry techniques, they provide equivalent or better data quality while speeding up the determination process significantly. Adding this automation to one’s laboratory workflow significantly reduces the amount of reagent used and analysts’ bench time. Ion chromatography (IC) is a well-established analytical method that expands the breadth of water analyses by offering distinct capabilities that include ultra-trace level determinations for a broad spectrum of analytes. Thermo Scientific Dionex IC Systems can be tailored to meet specific anion and cation analysis needs providing direct determination of multiple analytes in a single run and the ability to handle challenging sample matrices.
In this webinar, Timothy Traynor, Technical Specialist at Pace Analytical will present comprehensive water analysis workflows utilizing integrated discrete analyzers and Dionex IC systems and discuss their complementary benefits that offer high sensitivity, flexibility, and sample throughput, helping laboratories in Pace’s network to reduce overall cost per test and increase laboratory productivity.
Gary He, Product Marketing Manager, will also discuss the recent advances of Thermo Scientific Gallery Aqua Master Discrete Analyzers that enable more automation and better U.S. EPA compliance.
Dr. Carl Fisher, Product Marketing Manager, will conclude the session by providing a high-level overview of the tailored Thermo Scientific IC solutions that are integral to comprehensive water analysis.
<blockquote>
Presenter: Timothy Traynor (Technical Specialist, Pace Analytical)
</blockquote>
<blockquote>
Timothy Traynor has been a Technical Specialist in Pace’s IDEA Lab since 2019. Prior to his current role, Traynor has worked as a Chemist and a Metals Analyst at Pace, and a Chemist at the North Dakota Department of Health, among others. Traynor has a bachelor’s degree in Chemistry from Concordia College and a master’s degree in Theology from Saint John’s University.
</blockquote>
<blockquote>
Presenter: Gary He (Product Marketing Manager - Gallery Discrete Analyzers, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Currently serving as the Product Marketing Manager for the Gallery discrete analyzers within Thermo Fisher Scientific, Gary is responsible for leading the marketing programs to drive sustainable technology adoption in established applications such as water, nutrient, agricultural, food and beverage analyses, and in new adjacent applications, such as enzyme analysis for food and beverage, animal nutrition, grain processing, home care detergent, and biopharma.
</blockquote>
<blockquote>
Presenter: Carl Fisher (Product Marketing Manager - Ion Chromatography, Thermo Fisher Scientific)
</blockquote>
<blockquote>
In his current role, Carl is the Product Marketing Manager focusing on environmental applications for ion chromatography and sample preparation products . Prior to this, he spent several years as an analytical chemist as a member of the Applications Laboratory team, producing technical and application notes.
</blockquote>

Thermo Scientific: Expanding the breadth of water contaminant determinations with ion chromatography and discrete analysis

Expanding the breadth of water contaminant determinations with ion chromatography and discrete analysis

IC Systems can be tailored to meet anion and cation analysis needs providing direct determination of multiple analytes in a single and challenging matrices.

Water contaminant determinations with ion chromatography

Ion chromatography (IC) is a technique extensively used to analyze water samples from different sources, including drinking water, surface water, and wastewater.
Strict adherence to national or global standardized ion chromatography methods, including ISO, ASTM, and DIN, is critical to guarantee reliable and accurate results. Furthermore, standardization provides a unified analytical method for comparing and evaluating environmental monitoring data sets.
In this webinar, our European Senior Ion Chromatography Expert, Detlef Jensen, will explore the critical role of standardized ion chromatography methods in water contaminant analyses. 
ISO and ASTM IC procedures for the following water contaminants will be covered:
<ul>
<li>Disinfection byproducts e.g., bromate
</li>
<li>Perchlorate
</li>
<li>Chromate
</li>
<li>Cations, e.g., sodium, calcium, and ammonium
</li>
<li>Anions, e.g., chloride, nitrate, and fluoride
</li>
</ul>
Who should attend:
<ul>
<li>Environmental and water testing laboratories 
</li>
<li>Laboratory scientists and technicians testing using IC 
</li>
<li>Water and environmental analytical chemistry laboratory manager 
</li>
<li>Contract testing laboratories
</li>
</ul>
<blockquote>
Presenter: Detlef Jensen (Sr. Application Expert Applied Markets, EMEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Detlef holds a PhD in chemistry from the Technical University Berlin/Germany. For 30 years, he covered many positions at Dionex and Thermo Fisher Scientific. He is supporting our users and our team of field experts all over Europe with his deep knowledge of all expects of ion chromatography and its applications
</blockquote>

Thermo Scientific: Overview of standardized IC methods for water analysis

Overview of standardized IC methods for water analysis

Explore the critical role of standardized ion chromatography methods in water contaminant analyses.

This webinar highlights the importance of impurity testing in pharmaceuticals and how ion chromatography can provide excellent sensitivity and selectivity when testing for ionic impurities.
The management and control of pharmaceutical impurities is a matter of great concern and is an important part of drug development and regulatory assessment.
Current regulations and guidelines demand a comprehensive understanding of the impurity profile of pharmaceutical products, as their safety and quality can be impacted by these impurities.
The ICH quality guidelines ICH Q3A and Q3B address questions related to the regulation of impurities in the drug substance and drug product, whereas other ICH quality guidelines address specific types of impurities, such as residual solvents and mutagenic impurities.
This webinar explores the different kinds of impurities that may be present in pharmaceutical products and how ion chromatography (IC) is the analytical tool of choice to meet regulatory authorities’ requirements and in defining effective control strategies.
Learn how ion chromatography can help to automate impurities analysis workflows. Inline sample preparation techniques will also be discussed.
Key takeaways:
<ul>
<li>Understand the origins of impurities in pharmaceuticals and why impurity profiling is important
</li>
<li>Gain insight into the regulations surrounding impurities analysis in pharmaceuticals
</li>
<li>Learn about how ion chromatography makes an ideal technique for the analysis of ionic impurities, offering enhanced sensitivity and selectivity, and helping to automate and simplify workflow.
</li>
</ul>
<blockquote>
Presenter: Daniel Pereira (Senior Scientist, Regulatory Affairs, Hovione)
</blockquote>
<blockquote>
Daniel Pereira has been an analytical scientist at Hovione since 2018 and is currently a Senior Scientist of the chemical characterisation team in the R&amp;D Analytical Development Area. He has extensive expertise in the field of chemical characterisation techniques with focus in several chromatography techniques and in the development and optimisation of analytical methods following AQbD approach. Daniel holds a bachelor´s degree in Pharmacy from University of Rio de Janeiro, Brazil and a MSc in analytical chemistry form Minho University, Portugal.
</blockquote>
<blockquote>
Presenter: Linx Waclaski (IC Product Manager, Metrohm USA)
</blockquote>
<blockquote>
Linx Waclaski is the Product Manager for Ion Chromatography at Metrohm USA, in Riverview, Florida. He has years of experience in multiple chromatographic techniques, including ion chromatography, gas chromatography, liquid chromatography, and mass spectrometry, with extensive experience in R&amp;D and applications development. He has an MS in Forensic Science and a BA in Biochemistry from Duquesne University in Pittsburgh, Pennsylvania, US.
</blockquote>

Metrohm: Analysis of impurities in pharmaceuticals using ion chromatography

Analysis of impurities in pharmaceuticals using ion chromatography

The importance of impurity testing in pharmaceuticals and how ion chromatography can provide excellent sensitivity and selectivity.

Analysis of impurities in pharmaceuticals

Welcome to The Analytical Scientist’s Trends and Challenges in PFAS Analysis forum!
Per- and poly-fluorinated alkyl substances, or PFAS, are a family of synthetic substances used in everyday products, such as stain and grease-repellent coatings and have appeared in almost all foods and drinking water. Toxic at extremely low levels, regulators demand low detection limits, and the list of PFAS compounds under investigation continues to expand. 
We recently asked our readers for their thoughts on the challenges involved in PFAS analysis, whether current methods are up to the task, and what analytical advances may be on the horizon. 
Here, our panel of experts will discuss the survey findings and share their perspectives on the latest trends and best practices in PFAS analysis. 
The event will have three parts: 
<ul>
<li>Part I: A discussion of the results of our recent vendor-neutral audience survey (10-15 minutes).
</li>
<li>Part II: Technology/application-focused presentations from each of our speakers (15 minutes each).
</li>
<li>Part III: A chance for you to ask our speakers questions as part of an audience Q&amp;A (15 minutes).
</li>
</ul>
<blockquote>
Presenter: Day Powell (Environmental Application Specialist , Agilent)
</blockquote>
<blockquote>
Day has worked for Agilent as an Environmental Specialist for one year. Before this he worked for a major UK Water company (United Utilities) as a senior analyst responsible for developing new methodologies for both LC/MS and GC/MS applications.
</blockquote>
<blockquote>
Day has analysed PFAS for several years and helped United Utilities to gain UKAS accreditation for determining 47 PFAS by LC/MS in drinking water using direct injection to address the latest Drinking Water Inspectorate (DWI).
</blockquote>
<blockquote>
Presenter: Marcus Chadha (EMEA Applications Specialist Manager, Agilent) Dr. Marcus Chadha has worked for Agilent for seven years where he first worked as an LC/MS Field Application Specialist before moving on to being the EMEA Applications Specialist Manager in 2021.
</blockquote>
<blockquote>
In his roles he has collaborated with our Environmental and Food customers, developed new methods, and trained LC/TQ and LC-Q/TOF MS workflows to new and existing customers.
</blockquote>
<blockquote>
He now coordinates these activities using his team of GC/MS and LC/MS application specialists. Marcus is also responsible for the market specialisation activities for the environmental market across Europe.
</blockquote>
<blockquote>
Presenter: Linx Waclaski (Product Manager for Ion Chromatography, Metrohm USA)
</blockquote>
<blockquote>
Linx Waclaski is the Product Manager for Ion Chromatography at Metrohm USA, in Riverview, Florida. He has years of experience in multiple chromatographic techniques, including ion chromatography, gas chromatography, liquid chromatography, and mass spectrometry, with extensive experience in R&amp;D and applications development.
</blockquote>
<blockquote>
His chromatography applications experience extends across environmental, food safety, and life sciences industries. He has an M.S. in Forensic Science from Duquesne University.
</blockquote>
<blockquote>
Presenter: Ruth Marfil-Vega (Senior Market Manager - Environmental, Shimadzu)
</blockquote>
<blockquote>
Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants.
</blockquote>
<blockquote>
Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success.
</blockquote>
<blockquote>
She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.
</blockquote>

Agilent Technologies: Trends and Challenges in PFAS Analysis

Trends and Challenges in PFAS Analysis

Survey findings and perspectives on the latest trends and best practices in PFAS analysis.

In this on-demand webinar, Wai-Chi Man, Product Marketing Manager at Thermo Fisher Scientific, discussed the basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography with Mass Spectrometry (LC-MS), and complimentary to any Hydrophilic Interaction Liquid Chromatography (HILIC) analysis.
You can also expect to:
<ul>
<li>Enhance your understanding of the instrument requirements for IC and MS: which one is best for your application of interest.
</li>
<li>Learn how to set up the connectivity or simply switch from IC-MS to LC-MS.
</li>
<li>Explore the switch over from applications such as Polar Pesticides from Anionic to Cationic with one instrument set-up, and how it’s applicable to other applications such as Metabolomics.
</li>
</ul>
<blockquote>
Presenter: Wai-chi Man (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Wai-Chi Man, MRSC has been with Thermo Fisher Scientific since 2011. She started on the application sales support team but is now a product marketing manager. She has specialized in ion chromatography (IC) for more than 33 years, with over 36 years of experience in analytical chemistry in a wide variety of techniques. Before Thermo Fisher Scientific, she spent seven years at Wellcome Research supporting structural elucidation for medicinal chemists using a wide range of analytical instrumentation. Wai-Chi spent a total of 20 years in the pharma industry as part of GlaxoSmith Kline, from research to transferring methods to manufacturing. She worked on and was responsible for many drugs from anti-viral to respiratory in the market today.
</blockquote>
<blockquote>
Wai-Chi’s work at Thermo Fisher Scientific has supported a wide range of customers from various industries, providing IC support in method development, troubleshooting, sample preparation, trainings, seminars, events, and workshops. This experience has helped her build greater knowledge, skill and understanding within areas such as IC-MS, IC-ICP-MS, HPAE-PAD analysis, metabolomics, and polar pesticides.
</blockquote>

Thermo Scientific: Back to Basics – IC-MS 

Back to Basics – IC-MS 

The basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography.

Ultrapure water (UPW) is crucial for the production of superior-quality semiconductors, but ensuring final product quality can be a challenge without the right tools.
UPW undergoes extensive and costly purification, utilizing various physical and chemical treatments to meet increasingly rigorous purity standards. In the face of global water shortages, efficient UPW production is essential. Water recycling facilities at semiconductor plants are tasked with addressing this concern, regenerating wastewater back into UPW. However, the integrity of regenerated UPW must be verified to ensure final product quality. This is where online ion chromatography provides a novel advantage: detecting trace anions, cations, and silica contaminants in real-time down to parts-per-trillion levels. 
Join us for a conversation with two industry experts: Ben Greenwood, Senior Manager at Micron Technology&#39;s Chemistry Lab, and Dariana Martinez, Product Application Specialist at Metrohm USA. Together, they will delve into the pressing water challenges currently faced by the semiconductor industry. Additionally, they will shed light on the pivotal role of ultrapure water and the significance of real-time ion chromatography to ensure quality control. 
Key Learning Objectives:
<ul>
<li>Understand the pivotal role of ultrapure water (UPW) in semiconductor manufacturing and its direct impact on product quality and performance. 
</li>
<li>Gain insights into the scale of water consumption in the semiconductor industry and the pressing challenges faced in meeting increasingly rigorous purity standards. 
</li>
<li>Discuss the advanced analytical capabilities of online ion chromatography, and learn how it accurately measures anions, cations, and silica contaminants to verify UPW purity.
</li>
</ul>
Who Should Attend:
<ul>
<li>Anyone interested in leveraging automation for process optimization and near-real-time data analytics
</li>
<li>Process engineers and plant operators responsible for analytical testing and QA/QC Managers responsible for ensuring maximum product quality
</li>
<li>Those involved in adopting or implementing Industry 4.0 initiatives and/or focused on developing newer, more efficient manufacturing processes
</li>
</ul>
<blockquote>
Presenter: Ben Greenwood (Senior Manager, Micron Technology&#39;s Chemistry Lab)
</blockquote>
<blockquote>
Presenter: Dariana Martinez (Product Application Specialist, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

C&EN: Mastering UPW Quality with Real-Time Trace Ion Detection

Mastering UPW Quality with Real-Time Trace Ion Detection

The pressing water challenges currently faced by the semiconductor industry.

Join us and our partners as we co-host a webinar presenting the latest news about ion chromatography (IC) applied to anions analysis in water samples and learn how to:
<ul>
<li>New technology is used for the analysis of older contaminants, such as nitrate, nitrite, and other anions.
</li>
<li>Improve performance of methods EPA 300/300.1.
</li>
<li>Increase your productivity with Shimadzu’s trusted and durable hardware.
</li>
</ul>
We will host a Q&amp;A session after the presentation to address any questions or comments.
<blockquote>
Presenter: Ruth Marfil-Vega, PhD (Sr Market Manager – Environmental, Shimadzu Scientific Instruments)
Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success. She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.
</blockquote>
<blockquote>
Presenter: Nivesh Mittal (HPLC Product Manager, Shimadzu Scientific Instruments)
Dr. Nivesh Mittal has extensive experience in HPLC methods development and validation. Before joining Shimadzu in 2021, Nivesh was working in the pharmaceutical industry as a scientist, researching on the formulation development of nanoparticulate drug delivery systems for hard to manufacture compounds. Nivesh holds a BS and MS in Biotechnology from India, a PhD in Pharmaceutical Sciences from the University of Tennessee, and an MBA from the University of Memphis.
</blockquote>

Shimadzu Scientific Instruments: Ion Chromatography: A Break From the Tradition

Ion Chromatography: A Break From the Tradition

Join us and our partners as we co-host a webinar presenting the latest news about ion chromatography (IC) applied to anions analysis in water samples.

MagIC Net is the powerful software solution to control the ion chromatography instruments from Metrohm.
In this webinar you will learn about advanced functionalities to make your daily laboratory work more efficient such as the timer function, user-defined data monitoring, and the implementation of logical features in your IC method.
Participants in this webinar will also learn how to make their data handling more productive. Here, the relevant tools and features are advanced multi-parameter data search, control charts, and automatic database backup. A live Q&amp;A session will follow the webinar to address any questions you may have. 
<h4>Key learnings of this webinar:</h4>
<ul>
<li>Learning how to use the timer for automated start and stop options, how to implement a barcode reader, and how to set up user-defined monitoring parameters for system health checks as well as the validation of results and calibrations.
</li>
<li>Learning about logical decisions and how to implement them in your methods for inline dilution or automatic recalibration.
</li>
<li>Getting to know functions for advanced data handling and result management such as combining filters, customizing result overview, and minimizing manual steps for data backup.
</li>
<li>Learning how to personalize MagIC Net to adjust the software to your specific needs.
</li>
</ul>
Who should attend?
IC analysts, lab technicians, chemists, and everybody else currently using MagIC Net software.
<blockquote>
Presenter: Javier Espuelas, PhD (Product Specialist Ion Chromatography, Metrohm International Headquarters)
Javier Espuelas has been working for Metrohm for more than 20 years as Application Specialist developing analytcial methods and supporting customers in the field of ion chromatography. Before working for Metrohm, Javier obtained his PhD in chemistry from the Department of Analytical Chemistry of the University of Zaragoza in Spain, where he studied chromatography, electrochemistry, and spectroscopic analytical techniques.
</blockquote>
<blockquote>
Pesenter: Iris Reber (Sr. Product Specialist Ion Chromatography, Metrohm International Headquarters)
Iris is Senior Product Specialist at the Competence Center Ion Chromatography at Metrohm International Headquarters, Switzerland. She received her degree in chemistry from EPFL (Swiss Federal Institute of Technology, Lausanne).
Iris has worked for more than 12 years in the IC applications laboratory gaining extensive knowledge in this field.
</blockquote>

Metrohm: Showcasing advanced functionalities of MagIC Net

Showcasing advanced functionalities of MagIC Net

Showcasing advanced functionalities of MagIC Net software solution to control the ion chromatography instruments from Metrohm

LCMS webinars focusing on IC - page 6