Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026

🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.<h4>What You’ll Learn in This Episode:</h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3>Video Transcription</h3><h4>The Growing Challenge of Antibiotic Resistance</h4>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.<h4>Why the Bacterial Cell Wall Remains an Attractive Target</h4>The bacterial cell wall consists primarily of peptidoglycan, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.<h4>Targeting the Glycan Backbone</h4>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of small-molecule probes and inhibitors designed to recognize enzymes known as N-acetylglucosaminidases, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.Rather than beginning with purified enzymes, the researchers adopted a top-down phenotypic screening strategy. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.<h4>LC-MS as a Central Analytical Tool</h4>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.<h4>Sample Preparation Determines Data Quality</h4>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.<h4>Chemical Biology for Future Antibiotic Discovery</h4>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as chemical biology tools.By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.The research team is now expanding this strategy by combining their probes with proximity-labeling proteomics and mass spectrometry, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Agilent Technologies

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Shimadzu Corporation

This webinar will focus on the analytical methods and procedures being developed at NIST for accurately measuring cannabinoids (THC, THCA, CBD, etc.) in Cannabis samples and the solutions provided by Shimadzu Scientific Instruments.
NIST has started screening hemp plant and oil materials for 11 cannabinoids through the combination of liquid chromatography and a photodiode array detector (LC-PDA). Samples are extracted with methanol following the protocols approved in an AOAC Official Analytical Method for the quantitation of cannabinoids in Cannabis plant, concentrates, and oils. Samples at NIST are being analyzed using the external calibration approach for THC, THCA, and total THC with the “Cannabis Analyzer” from Shimadzu. 
After initial screenings, potential hemp RMs and CannaQAP materials will be well characterized using isotope dilution (ID) gas chromatography – mass spectrometry (GC-MS) and LC with tandem mass spectrometry (MS/MS).
<blockquote>
Presenter: Walter Brent Wilson (Research Chemist, National Institute of Standards and Technology (NIST))
</blockquote>
<blockquote>
Walter’s interests are in the development of new analytical methods for the determination of cannabinoids in complex Cannabis matrixes: dried plant, oils, extracts, concentrates, edibles, etc. through the use of liquid chromatography coupled to photodiode array detector or tandem mass spectrometry and gas chromatography coupled to mass spectrometry. Walter’s employment at NIST began as a postdoctoral associate with the measurement of organic contaminants in environmental and combustion related SRMs. Walter has also been heavily involved in the development of methods and measurements of nicotine and other alkaloids in tobacco products. In 2019, Walter began coordinating the <a href="https://www.nist.gov/programs-projects/nist-tools-cannabis-laboratory-quality-assurance">Cannabis research program in the Chemical Science Division at NIST</a> with a focus on developing Cannabis reference materials and a Quality Assurance Program (<a href="https://qa.nist.gov/cannaqap/">CannaQAP</a>).
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<blockquote>
Presenter: Melissa M. Phillips (Research Chemist, National Institute of Standards and Technology (NIST))
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Melissa&#39;s interests are in the development of new analytical methods for the determination of marker compounds, vitamins, and contaminants in dietary supplements and foods, as well as improving the measurement capabilities of the dietary supplement and food communities. Melissa&#39;s employment at NIST began as a postdoctoral associate with the measurement of organic acids, anthocyanidins, and flavonols in a suite of Vaccinium berry SRMs (cranberry, blueberry, and bilberry). Melissa has also been heavily involved in the development of methods and measurement of water-soluble vitamins (B1, B2, B3, B5, B6, choline, and carnitine) in numerous food SRMs. Melissa was a co-coordinator for the former Dietary Supplement Laboratory Quality Assurance Program (DSQAP) from 2009 to 2017. She is currently a co-coordinator of the <a href="https://www.nist.gov/programs-projects/health-assessment-measurements-quality-assurance-program">Health Assessment Measurements Quality Assurance Program</a> (HAMQAP) and also the <a href="https://www.nist.gov/programs-projects/measurements-and-standards-support-nutrition-labeling">food and nutrition SRM program</a>. From November 2016 through June 2017, Melissa was detailed to the Office of Reference Materials. In 2018, Melissa began formulation of evolving efforts into a grassroots Food Safety Program within the Chemical Sciences Division, and chaired the NIST Food Safety Workshop in October 2019.
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Presenter: Rachel Lieberman (Marketing Manager for Forensic Science, Shimadzu Scientific Instruments)
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Rachel received her B.S. degree in Chemistry with minor in Math in 2004 from the University of Cincinnati and her Ph.D. in Analytical Chemistry from University of North Carolina – Chapel Hill under the supervision of Prof. James Jorgenson. In May of 2011, Rachel joined Shimadzu as an Application Scientist in LC and LC-MS/MS at corporate headquarters in Columbia, MD. In 2013, Rachel became a Sr. LCMS Product Specialist and was promoted to LCMS Product coordinator in April of 2017. In October of 2017, Rachel became the first Marketing Manager for Forensic Science at Shimadzu Scientific Instruments.
</blockquote>

Shimadzu ASMS 2020: NIST Tools for Confidently Distinguishing Between Hemp and Marijuana

ASMS 2020: NIST Tools for Confidently Distinguishing Between Hemp and Marijuana

Phenomenex has recently launched a unique web series unlike anything they have done before. “Inside the Lab” invites viewers to not only learn from a panel of experts of the related topic, but allows you to ask questions in real time and become a part of the discussion.
You may have attended a <a href="https://www.phenomenex.com/Info/Page/upcoming-webinars">webinar Phenomenex</a> has hosted before, but this will be something different. This series is an opportunity for you to virtually talk face-to-face with the people who design and create Phenomenex products and consider chromatography not just a science, but an art. You’ll be able to ask them anything! But of course, we can only guarantee expertise in chromatography.

Phenomenex: Inside the Lab with Phenomenex - GC

Inside the Lab with Phenomenex - GC

Reversed phase is the most common mode of HPLC but if it’s not working for you, don’t despair, there are other options. Perhaps HILIC, Chiral, IEX, GPC/SEC, Affinity, SFC, or HIC might be better suited to your sample type.
In this seminar, we will look at additional chromatographic techniques and discuss:
<ul>
<li>Mechanism of separation
</li>
<li>Column selection and available chemistries
</li>
<li>Typical method and eluent conditions
</li>
</ul>
Becoming familiar with other modes of chromatography and investigating alternative chemistry options can result in an improved separation or provide additional information for your sample.
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Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
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Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: There is More to HPLC Than Reversed Phase; Exploring Other Options

There is More to HPLC Than Reversed Phase; Exploring Other Options

There is More to HPLC: Exploring Other Options

Whether your chromatography suddenly changes or you’re not able to reproduce a method from, e.g. a pharmacopeia, isolating the source of the problem can be a challenge.
In this seminar we will develop your troubleshooting skills as we:
Discuss common chromatography problems
<ul>
<li>Pressure
</li>
<li>Peak Shape
</li>
<li>Retention
</li>
<li>Look at how to isolate the problem
</li>
<li>Chemistry related
</li>
<li>Hardware/software
</li>
</ul>
We will also talk about ways to prevent future problems.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: What's Wrong With My Chromatography? Is it the Column, Method or Instrument

What's Wrong With My Chromatography? Is it the Column, Method or Instrument

Develop your troubleshooting skills. Common chromatography problems.

What's Wrong With My Chromatography?

A rugged HPLC method is one that produces reproducible analytical results under actual use conditions.
To produce results that are accurate, repeatable, and reproducible an HPLC separation must either be relatively insensitive to small variations in analysis conditions, or those variations must be identified and controlled satisfactorily. This is termed method robustness. It is a required validation criterion for analytical methods submitted to national and international regulatory agencies.
In this presentation we will discuss:
<ul>
<li>Important factors that affect the robustness and ruggedness of HPLC separations
</li>
<li>Describe the importance of and the advantages of considering these factors as part of the method development process
</li>
<li>The development of a robust method is not only cost effective in the long term, but it also increases the likelihood of successful method transfer to other in-house or contract analytical laboratories.
</li>
</ul>
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Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: HPLC Separation Robustness and Ruggedness - Variables to Evaluate during Method Development

HPLC Separation Robustness and Ruggedness - Variables to Evaluate during Method Development

Important factors that affect the robustness and ruggedness of HPLC separations.

Variables to Evaluate during Method Development

Whether you’re new to HPLC or have been doing it for years, there are always things we can learn.
There are many pitfalls or “gotchas”. Even the issue of what tubing and fittings are compatible and which are not can be a serious impediment to a successful HPLC separation.
In this talk, we will discuss:
<ul>
<li>The importance of connections
</li>
<li>Mobile phase considerations
</li>
<li>Quality
</li>
<li>Preventing microbial growth
</li>
<li>Sample/solvent compatibility
</li>
<li>Column characteristics; their importance and limitations
</li>
<li>The importance of sample prep
</li>
<li>Avoiding these pitfalls can help you achieve long-term, reliable results.
</li>
</ul>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: HPLC - There's a Lot to Learn - Tips for Avoiding Pitfalls

HPLC - There's a Lot to Learn - Tips for Avoiding Pitfalls

Whether you’re new to HPLC or have been doing it for years, there are always things we can learn.

Developing reliable gradient methods is more challenging compared to isocratic methods.
In this seminar we will discuss:
<ul>
<li>Why and when a gradient separation makes sense
</li>
<li>Isocratic vs. gradient
</li>
<li>Sample types
</li>
<li>How to develop a reliable gradient method
</li>
<li>What parameters can maximize gradient resolution
</li>
</ul>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: Gradient, Reversed Phase HPLC - The Why, What, When and How

Gradient, Reversed Phase HPLC - The Why, What, When and How

Developing reliable gradient methods is more challenging compared to isocratic methods.

Preventing pressure, peak shape, and retention time problems saves you the time and aggravation of troubleshooting. Join us on a journey through the LC system from the solvent bottles through the detector.
During this free webinar we will make stops to explore:
<ul>
<li>The role your instrument plays
</li>
<li>The benefits of incorporating sample prep into your workflow
</li>
</ul>
The importance of selecting the best column for your analysis Problems can introduce unexpected detours. Plan ahead to insure your sample arrives as expected at its destination.
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Agilent Technologies: Around the LC System in 45 Slides!

Around the LC System in 45 Slides!

LC system from the solvent bottles through the detector.

Whether your chromatography suddenly changes or you’re not able to reproduce a method from, e.g. a pharmacopeia, isolating the source of the problem can be a challenge.
In this seminar we will develop your troubleshooting skills as we:
<ul>
<li>Discuss common chromatography problems
</li>
<li>Pressure
</li>
<li>Peak Shape
</li>
<li>Retention
</li>
<li>Look at how to isolate the problem
</li>
<li>Chemistry related
</li>
<li>Hardware/software
</li>
</ul>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: Taking the Trouble Out of Troubleshooting - Is it the Column, Method or Instrument

Taking the Trouble Out of Troubleshooting - Is it the Column, Method or Instrument

Taking the Trouble Out of Troubleshooting

Data quality and data integrity are critical in key decisions supporting the safety and efficacy of pharmaceutical products. Regulatory observations in pharmaceutical laboratories routinely cite concerns regarding the suitability of analytical methods, particularly chromatographic methods that require analysts to intervene, adjust or repeat testing.
Inspired by a suite of new ICH guidelines, chromatographers are now managing methods using a lifecycle approach to improve the consistency, ruggedness and quality of separations to enable right-first-time analyses. Development and deployment of these analytical methods to modern, routine-use HPLC platforms mitigates the risk of invalidated data, continually adjusted or manual peak integration, and system suitability failures.
The webinar hosts will examine key challenges faced in the routine analysis laboratory and demonstrate method transfer to the new Waters Arc HPLC System. The Arc HPLC is designed to receive transferred methods seamlessly and run existing HPLC methods with improved robustness and precision over traditional HPLCs, avoiding system suitability failures and the invalidation of results. The Arc family of LC systems, when paired with appropriate column technology and gold standard, compliant-ready Empower Software, combine to offer robust performance in routine analysis, facilitating data review, eliminating burdensome quality or compliance documentation, and improving the overall quality of laboratory data.
<h3>Key learning objectives</h3>
<ul>
<li>Current trends in regulatory observations referencing chromatography analyses</li>
<li>How analytical method performance can be a root cause of data integrity concerns</li>
<li>How new ICH guidelines will influence how chromatographic methods are developed and documented, resulting in more stringent system suitability and reproducibility requirements</li>
<li>How improvements in the performance and ruggedness of HPLC instruments can improve data quality</li>
</ul>
<h3>Who should attend</h3>
<ul>
<li>Pharmaceutical directors, managers and analysts who wish to ensure data quality in the QC/routine analysis laboratory</li>
</ul>
<blockquote>
Presenter: Heather Longden (Senior Marketing Manager, Pharmaceutical Intelligence, Waters Corporation)
</blockquote>
<blockquote>
After a number of years in training, supporting and selling Waters Software, Heather&#39;s current role is as a specialist in compliance to e-record regulations, acting as a resource to the global informatics community at Waters and the users of all Waters informatics products. This involves helping design the compliance into Waters informatics products and services, attending and presenting at specialist conferences, keeping up to date on the latest interpretations and amendments to the regulations and disseminating this information to the Waters field and corporate organizations.
</blockquote>
<blockquote>
Presenter: Paula Hong, PhD (Principal Consulting Scientist, Waters Corporation)
</blockquote>
<blockquote>
Dr. Paula Hong is a principal consulting scientist at Waters Corporation in Milford, MA. She received her Ph.D. in inorganic chemistry from the University of Pennsylvania (Philadelphia, PA) and began her career as an applications chemist at Waters Corporation over 15 years ago in the area of bioseparations. She has authored numerous journal articles, white papers, and application notes on both LC and SFC techniques. Her more recent work has focused on LC/MS applications, method transfer and method lifecycle management.
</blockquote>
<blockquote>
Moderator: Finn Price (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Fionnbhar Price is a member of the SelectScience Editorial Team, who plays a core role in sourcing and publishing content for the site, with a particular focus on the field of applied chemistry. He has an MChem in Chemistry from Cardiff University, UK, and undertook a year in research characterizing bacterial protein.
</blockquote>

Pixabay/Tumisu: Ensuring Data Quality and Addressing Chromatographic Challenges in Routine Pharmaceutical Analysis: Tools for Success

LCMS webinars focusing on HPLC - page 74