Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilnt Technologies: UV/Vis Software Bootcamp

UV/Vis Software Bootcamp

Agilent Technologies Molecular Instrumentation and Applications...
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>

Demystifying Molecular Instrumentation and Applications

Agilent Technologies Molecular Instrumentation and Applications...

In this third session of a four-part ‘Fundamentals of Spectroscopy’ course, having previously covered the ‘why’, we’ll now focus on the ‘how’. There is currently a dizzying array of tools available for spectroscopy, but the fundamental goal remains the same.
Here we will discuss the current state-of-the-art common spectroscopy components – spectrometers, detectors, and associated optics – as well as their historical development. We will discuss some of the technological breakthroughs that have made the heroic measurements of yesterday commonplace practice today, as well as how different equipment has evolved to suit the particular needs of different experiments.
Key learning objectives
<ul>
<li>Discover the function of a spectrograph and how the earliest spectrographs achieved this</li>
<li>Understand the difference between a prism and grating spectrograph and learn why grating spectrographs are significantly more common</li>
<li>Consider what is meant by “dispersion” in a spectrometer, what is spectral resolution, and how the two concepts relate to each other</li>
</ul>
Who should attend?
<ul>
<li>Academic researchers</li>
<li>R&amp;D scientists</li>
<li>Lab technicians</li>
<li>Principal investigators</li>
<li>Laboratory, facilities or corporate managers</li>
<li>Postdoctoral research assistants</li>
<li>Undergraduate and PhD students</li>
<li>Engineers or software engineers</li>
<li>Teaching fellows</li>
</ul>
<blockquote>
Presenter: Adam Wise (Andor Technology)
</blockquote>
<blockquote>
Adam Wise is an applications scientist at Andor Technology, specializing in spectroscopy and scientific cameras. He earned his PhD working with John Grey at the University of New Mexico, studying Raman spectroscopy of organic semiconductors. After his postdoctoral research at UMass Amherst, Wise entered the field of optical instrumentation, and continues to support research by helping scientists get the right cutting-edge tools.
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<blockquote>
Presenter: Georgina Wynne Hughes (Editorial Team)
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Georgina studied at Birmingham University where she gained a BSc in Biomedical Science, and an MSc by Research in Cancer and Genomic Sciences, which focused on defective autophagy in rare neurodegenerative diseases. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Optimizing equipment for optical spectroscopy

Optimizing equipment for optical spectroscopy

In this third session of a four-part ‘Fundamentals of Spectroscopy’ course, having previously covered the ‘why’, we’ll now focus on the ‘how’.

During this second session of a four-part ‘Fundamentals of Spectroscopy’ course, we will break down some common spectroscopic techniques (UV/VIS absorption spectroscopy, photoluminescence, and Raman scattering amongst others), examine how they work at a relatively low level, and discuss what they can be used to learn in different situations. We will pay special attention to the historical developments of these techniques, particularly the scientific and economic motivations that drove the people who created and refined them.
Key learning objectives
<ul>
<li>Understand the concept of absorption spectroscopy, the design of an absorption spectrometer, and see how UV-VIS absorption spectroscopy can be used in quantifying protein concentration.
</li>
<li>Differentiate between the different types of luminescence, specifically photoluminescence vs. fluorescence and understand the basics of fluorescence and phosphorescence spectrometers. Become familiar with applications using time-resolved fluorescence, solvatochromic shifts and Förster resonance energy transfer.
</li>
<li>Learn the basics of Raman spectroscopy, and the design of a Raman spectrometer. Understand the units used in Raman spectroscopy and learn how Raman specificity can be used to measure various physical and chemical properties in materials.
</li>
</ul>
<blockquote>
Presenter Shayne Harrel (Andor Technology)
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Shayne Harrel is a spectroscopy specialist for the Americas at Andor. He holds a PhD in physical chemistry from Yale University where he studied dynamical processes in charge transfer systems, magnetic materials and semiconductors using time-resolved terahertz spectroscopy. Prior to joining Andor, he was a senior applications engineer at MKS Instruments and has held previous positions as Chief Scientist-Americas for Izon Science, Senior Applications Scientist for Olympus Corporation, and as a chemist for the US Food and Drug Administration.
</blockquote>
<blockquote>
Presenter Georgina Wynne Hughes (Editorial Team)
</blockquote>
<blockquote>
Georgina studied at Birmingham University where she gained a BSc in Biomedical Science, and an MSc by Research in Cancer and Genomic Sciences, which focused on defective autophagy in rare neurodegenerative diseases. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Spectroscopy techniques in practice: Methods and motivations

Spectroscopy techniques in practice: Methods and motivations

We will break down UV/VIS absorption spectroscopy, photoluminescence, and Raman scattering amongst others and examine how they work.

UV-VIS typical maintenance and some common validation tests will be discussed.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Maintaining Your UV-VIS Spectrometer

Maintaining Your UV-VIS Spectrometer

UV-VIS typical maintenance and some common validation tests will be discussed.

This webinar will guide you on the best practices, tools, and techniques needed to get the most reliable, verifiable insights into your optical components. Appropriate choice of technique and methodology is critical to getting the information you need to improve design, verify product performance, and accelerate manufacturing.
We will explore the subtleties of reflection and transmission measurements at normal incidence and at oblique angles, emphasizing high absorbance, optical density, linearity, and effects of incident beam collimation. We will discuss diffuse scattering measurements and automation/robotics to broaden quality assurance programs and speed up analysis. 
The webinar is relevant to developers of optics, photonics, and electro-optical or semiconductor devices such as visual displays, laser-based range detection (LiDAR), optical communications systems and light-based scientific instrumentation. Improve your understanding of the link between your desired outcomes and the spectrophotometric approaches needed to achieve those insights. 
Key Learning Objectives:
<ul>
<li>The best spectrophotometric practices to extract deeper insights into the characteristics of optical samples.</li>
<li>How to overcome the limitations of traditional spectrophotometric tools and verify outcomes.</li>
<li>Accelerating QA/QC of optics in a production environment.</li>
</ul>
Who Should Attend:
<ul>
<li>Commercial or Academic organizations in the fields of optics, photonics, electro-optical, or semiconductors</li>
<li>Laboratory Managers </li>
<li>Scientist/Staff Scientists</li>
<li>Lab Technician/Technologist</li>
<li>Analyst</li>
<li>Project Manager</li>
<li>SME Directors, Core Lab/ Facility Managers </li>
<li>Under/Post Graduate Researchers</li>
</ul>
<blockquote>
Presenter: Travis Burt (Global Product Manager – Cary UV-Vis-NIR Spectrophotometers, Agilent Technologies)
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Presenter: Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)
</blockquote>

C&EN: Optimizing Optics Using Advanced Spectrophotometry

Optimizing Optics Using Advanced Spectrophotometry

This webinar will guide you on the best practices, tools, and techniques needed to get the most reliable, verifiable insights into your optical components.

There are many different applications which can be run on an UV-Vis-nir instrument. Within these applications there are a number of instrument parameters which the user can adjust to improve the quality of the data collected or use to optimize the collection time.
A discussion will be held around both available applications and pertinent instrument parameters.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>

Agilent Technologies: Navigating Your Way through Parameters and Applications of an UV-Vis-nir Instrument

Navigating Your Way through Parameters and Applications of an UV-Vis-nir Instrument

A discussion will be held around both available applications and pertinent instrument parameters.

Parameters and Applications of an UV-Vis-nir Instrument

When creating nanoparticles as fast as possible, researchers need analytical techniques which keep up. Whether that means keeping up with tons of new particle varieties or just being able to run batch after batch made during process optimization, gathering payload concentration and nanoparticle size data always sucks up lots of time. Current methods for quantification are often complex, requiring standard curves and breaking down your particle. Getting size information is usually a slow one-at-a-time job and consumes high volumes of sample.Stunner now does the heavy lifting for you to produce high-throughput, non-destructive nanoparticle quant and sizing. Stunner’s intelligent UV/Vis-based quantification measures your payload as-is, without dyes or destructive workflows, and pairs it with high-throughput DLS to deliver size and aggregation info – all on just 2 µL of sample. In this webinar, we’ll show you how easy it is to customize the quantification of your nanoparticle, no matter what’s loaded inside: RNA, DNA, protein, or small molecules. Step up your nanoparticle characterization game with reagent-free, standard-free, and hassle-free quant and sizing on Stunner.<blockquote>Presenter:&nbsp;Nelis Denys (Product Manager, Unagi, Unchained Labs)Moderator: Kevin Lance PhD (Marketing Manager, Analytical Instruments, Unchained Labs)</blockquote>

Unchained Labs: Level up your nanoparticle quant and sizing on Stunner

Level up your nanoparticle quant and sizing on Stunner

Speed up nanoparticle quant and sizing with Stunner. Get fast, non-destructive data on size and payload from just 2 µL—no dyes, no standards, no hassle.

<h5>The Problem:</h5>Wringing every drop of data from a tiny sample volume of your AAV can be the difference between understanding what’s really going on with your sample and leaving questions unanswered. In AAV characterization, there’s always a need for rapid answers on metrics like capsid titer, genome titer, empty/full ratio, aggregation, and total protein or DNA quant. Together all that data can paint the picture of what’s going on in your sample and your process, but with so many characterization options available knowing how they all stack up can make it a challenge to pick the right analytical tool.<h5>The Solution:</h5>To get the most data from the smallest sample volume, Stunner combines UV/Vis, static and dynamic light scattering (SLS &amp; DLS) to give a full read-out in about a minute on just 2 µL of AAV sample. Uniting these three techniques creates a uniquely robust approach to a classic light scattering method that goes toe-to-toe with other techniques. In this webinar we’ll cover how Stunner is being used and published to show how it stacks up in the world of AAV analytics and process development.<h5>The Proof:</h5>We’ll check out how Stunner’s technologies provide a robust method useful for rapid sample QC, optimizing downstream purification chromatography, and improving the workflow of other common analytical assays. Whether you’re working with fractions, coming off a purification column or trying to pick the best sample for a sample-hungry assay, we’ll show data and case studies on how well Stunner can accelerate your AAV characterization to save you sample and time.<blockquote>Presenter:&nbsp;Kevin Lance PhD (Marketing Manager, Analytical Instruments, Unchained Labs)Moderator: Martha Perez, PhD (Marketing Director, LNP Solutions, Unchained Labs)</blockquote>

Unchained Labs: Uncover way more AAV data in one shot on Stunner

Uncover way more AAV data in one shot on Stunner

Need more data from less AAV? See how Stunner delivers genome titer, empty/full ratio, and more in 2 µL—fast, accurate analytics without draining your sample.

<h5>The Problem&nbsp;</h5>Working with ADCs can really be a nightmare. They are sticky and throw up a fight when you try to characterize them: first you never have enough for all tests you need to run and then they are likely to aggregate before you have a chance to complete your testing.<h5>The Solution&nbsp;</h5>Unchained Labs is all about providing solutions that only need a few microliters of sample, combine a bunch of measurements into one experiment and get you your data fast. Stunner quantifies proteins and labelling efficiency, plus it gauges sample quality by assessing aggregates. Uncle monitors stability of your biologic and is your go-to when testing formulations, both in quick thermal ramps and in long term isothermal tests. Honeybun treats you to viscosity data with small volumes and takes no time. Sweet.<h5>The Proof&nbsp;</h5>Join our webinar and check out concentration, labeling, stability, and viscosity data of a mAb and its conjugate. We'll show you how to use Unchained Labs tools to squeeze all that info from only a couple of microliters of sample – to give you the full scoop, and no nightmares. &nbsp;<blockquote>Presenter:&nbsp;Andree Mueller (Senior Product Manager for Aunty and Honeybun, Unchained Labs)</blockquote>

Unchained Labs: Get the full scoop on your ADC from microliters

Get the full scoop on your ADC from microliters

Join our webinar and check out concentration, labeling, stability, and viscosity data of a mAb and its conjugate. 

LCMS webinars focusing on UV-Vis - page 8