<div><p><strong>Per- and polyfluoroalkyl substances (PFAS) represent a vast class of more than 3,000 synthetic compounds containing perfluorinated chains (with the general formula CnF₂n+₁COOH), commonly utilized in a wide range of consumer products and industrial processes.</strong></p><p>The unique structure of PFAS, characterized by multiple carbon-fluorine (C-F) bonds, contributes to their <strong>exceptional chemical stability and resistance</strong> to degradation through environmental or biological processes. This durability makes them some of the <strong>most persistent pollutants in the environment</strong>. Their resistance to water and oil has led to widespread use in diverse applications, including water- and stain-repellent coatings for textiles, paints, personal care items, firefighting foams, fluoropolymer-based lubricants and additives, as well as grease- and water-resistant food packaging. Due to their extensive use, PFAS have been found in various environmental and biological samples, such as <strong>food, drinking water, wastewater, and human blood</strong>.</p></div><div><div><div><div><h3><strong>Why PFAS Testing Is Crucial?</strong></h3></div></div></div></div><div><p>Like many other chemicals, PFAS can cause<strong> </strong>various <strong>harmful health effects</strong>, depending on factors such as the level, duration, and route of exposure, as well as individual characteristics like age, sex, ethnicity, overall health, and genetic background. PFAS exposure has been linked to various health effects in both sexes, with some impacts supported by high certainty evidence—such as <strong>thyroid disease, liver damage, kidney cancer, and testicular cancer</strong>.</p><p>In females, PFAS may also affect pregnancy outcomes and fetal development, including increased miscarriage risk, reduced birth weight, and delayed mammary gland development. Because of their toxic nature, these compounds have been classified as “persistent organic pollutants” by the<strong> Stockholm Convention</strong> (including PFOS, PFOA, PFHxS, and their salts) and by the <strong>European Commission</strong> (including PFOA, PFOS, PFNA, and PFHxA). Their durability, chemical stability, harmful effects, and tendency to build up in living organisms make them a serious risk to both environmental and human health; hence, PFAS testing is crucial for monitoring and mitigating these threats.</p></div><div><div><div><div><h3><strong>PFAS Testing Techniques</strong></h3></div></div></div></div><div><p>PFAS testing employs advanced techniques to detect, identify, and quantify both known and emerging compounds across diverse environmental matrices.</p><h5><strong>Liquid Chromatography-Mass Spectrometry (LC-MS/MS)</strong></h5><p>LC-MS is a <strong>commonly used PFAS testing method</strong>. Samples are first processed using suitable preparation techniques, such as <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50"><strong>solid phase extraction </strong></a>(SPE). The resulting extracted samples are then examined using LC/MS/MS with calibration performed via standards. Target analytes are identified with help of isotopically labeled surrogate standards. . Additionally, product ion ratios are compared with those from standards to support identification. Once confirmed, the concentration of each identified target compound is determined based on the response of its main product ion using external standard calibration.</p></div><div><div><div><div><h4><strong>Gas Chromatography-Mass Spectrometry (GC-MS)</strong></h4></div></div></div></div><div><p>Non targeted analysis of PFAS has largely been dominated by LC-MS methods, although GC-MS can access a complementary chemical space, especially for volatile and semivolatile PFAS. Studies show that LC‑MS covers most ionic PFAS; GC‑MS captures neutral/volatile precursors less amenable to LC, highlighting the need for GC-based approaches. GC is particularly valuable for identifying novel PFAS and transformation products from sources like manufacturing waste, incineration, wastewater, and consumer products.</p></div><div><div><div><div><h4><strong>Total Organic Fluorine (TOF)</strong></h4></div></div></div></div><div><p>Total Organic Fluorine (TOF) analysis is an indirect method used to estimate the overall burden of per- and polyfluoroalkyl substances (PFAS) and other organofluorine compounds in a sample. Instead of targeting individual PFAS molecules, TOF methods quantify the sum of organically bound fluorine.</p><p>Because this process measures the organic fluorine present within a sample, it provides a helpful bulk measurement of fluorine from all PFAS present, including those which may not be covered by targeted LC-MS/MS methods.</p></div><div><div><div><div><h4><strong>High Resolution Mass Spectrometry (HRMS)</strong></h4></div></div></div></div><div><p>HRMS is increasingly used to detect emerging PFAS, with two main strategies applied for identification: suspect screening (SS) and non-target screening (NTS). SS is a more direct method, particularly effective when tandem mass spectrometry (MS/MS) reference spectra are available. In contrast, NTS is critical for uncovering new PFAS compounds in the absence of a suspect list, using fragment-based or homologue-based techniques.</p><p>HRMS contributes significantly by offering precise mass measurements, which are essential for identifying unknown substances. Since PFAS often form homologous series, patterns in accurate mass, retention time, and common fragments (such as CF₂, C₂F₄, and CF₂O) help in their recognition.</p></div><div><div><div><div><h3><strong>Choosing the Right Testing Method</strong></h3></div></div></div></div><div><p>C-MS/MS is the primary technique used for targeted PFAS analysis due to its high sensitivity and accuracy. It is validated by the U.S. EPA through established methods such as 1633, 533, and 537.1 for detecting PFAS in various environmental and drinking water samples. For laboratories seeking reliable and compliant workflows, choosing a comprehensive <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/industries/pfas">P<strong>FAS analysis solution</strong></a> that aligns with these regulatory methods is essential for accurate detection and quantification.</p><ul><li><strong>EPA Method 1633</strong> is designed for use under the Clean Water Act (CWA) to measure the levels of 40 different PFAS in various sample types including water, soil, biosolids, sediment, and biological tissue, using <strong>LC-MS/MS</strong>. Weak anion exchange solid phase extraction (WAX-SPE) is employed for extracting various sample matrix types, and PFAS compounds are quantified through calibration with isotopically labeled internal standards.<br>&nbsp;</li><li><strong>EPA Method 533</strong> utilizes weak anion exchange solid phase extraction (WAX-SPE) followed by LC-MS/MS to detect 25 specific PFAS in drinking water, including short chain PFAs. This method employs tandem mass spectrometry and isotope dilution to improve analytical selectivity.<br>&nbsp;</li><li><strong>Method 537.1 </strong>involves extraction via SDBL reversed phase SPE combined with LC-MS/MS for detection of 18 specific PFAs compounds, including the novel GenX compound. This method involves <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/documents/pfas-in-drinking-water">PFAS detection in drinking water</a> samples.</li></ul></div>

Guide to PFAS Testing Methods

<p><span><strong>In analytical chemistry, achieving reliable results often depends on attention to seemingly minor details. One such detail is the use of micro inserts—small inserts placed inside standard HPLC vials that can significantly improve analytical performance when working with limited sample volumes. Although modest in size, these accessories help laboratories maximize sample utilization, improve reproducibility, and enhance measurement sensitivity.</strong></span></p><p><span>For laboratories handling valuable, scarce, or low-volume samples, incorporating micro inserts into daily workflows can be a simple yet highly effective way to improve chromatographic results.</span></p><h4><span><strong>Understanding Micro Inserts and Their Importance</strong></span></h4><p><span>Micro inserts are small vessels designed to fit inside conventional 1.5 mL autosampler vials. Their primary purpose is to reduce the effective sample volume required for analysis while ensuring that even very small quantities of sample remain accessible to the autosampler needle.</span></p><p><span>This capability is particularly valuable in applications where sample availability is limited. Whether working with pharmaceutical compounds, environmental extracts, forensic specimens, or food safety samples, analysts often need to obtain reliable results from only a few microliters of material.</span></p><p><span>By concentrating the sample into a smaller space, micro inserts help improve sample recovery and analytical sensitivity. As a result, laboratories can detect low-level compounds more effectively while minimizing unnecessary sample consumption.</span></p><p><span>Ultimately, micro inserts contribute to more efficient analyses, improved data quality, and better utilization of valuable samples.</span></p><h3><span><strong>Key Benefits of Using Micro Inserts in HPLC</strong></span></h3><p><span>Although simple in design, micro inserts offer several important advantages that can improve both analytical performance and laboratory productivity.</span></p><h4><span><strong>Optimized Use of Small Sample Volumes</strong></span></h4><p><span>One of the primary reasons for using micro inserts is their ability to accommodate very small sample quantities. Samples in the low-microliter range can be analyzed efficiently without dilution or excessive waste.</span></p><p><span>This is especially beneficial when dealing with expensive, rare, or difficult-to-obtain materials, as it ensures that nearly all available sample can be used for analysis rather than remaining inaccessible within the vial.</span></p><h4><span><strong>Protection Against Sample Loss Through Evaporation</strong></span></h4><p><span>Small liquid volumes are particularly susceptible to evaporation, which can alter sample concentration and compromise analytical accuracy.</span></p><p><span>Micro inserts help minimize this risk by reducing exposed surface area and providing a more contained environment for the sample. This added protection is particularly useful when handling volatile compounds that may otherwise be lost before analysis.</span></p><h4><span><strong>Improved Sensitivity and Analytical Precision</strong></span></h4><p><span>The design of micro inserts allows the autosampler to access a greater proportion of the available sample. More efficient sample uptake often translates into improved chromatographic performance.</span></p><p><span>Benefits may include:</span></p><ul><li><span>Better peak shapes</span></li><li><span>Increased signal intensity</span></li><li><span>Enhanced signal-to-noise ratios</span></li><li><span>Improved reproducibility</span></li></ul><p><span>These advantages become especially important when quantifying trace-level analytes or working near detection limits.</span></p><h4><span><strong>Lower Risk of Carryover</strong></span></h4><p><span>Carryover remains a common concern in chromatographic analysis, particularly when highly concentrated samples are followed by low-level samples.</span></p><p><span>Because micro inserts confine the sample to a smaller and more controlled space, they can help reduce residual contamination between injections. The result is cleaner analyses and greater confidence in reported data.</span></p><h4><span><strong>Compatibility with Automated Systems</strong></span></h4><p><span>Modern laboratories increasingly rely on autosamplers and automated workflows to maximize productivity.</span></p><p><span>Micro inserts are specifically designed to function seamlessly within these systems, enabling reliable sample positioning and injection while reducing the likelihood of handling errors. This makes them particularly valuable in high-throughput environments where consistency and efficiency are critical.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/knauer_lc_lab_application_development_1920x1280_ee0a43ad6e.webp" alt="KNAUER: HPLC Lab - Application Development" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_knauer_lc_lab_application_development_1920x1280_ee0a43ad6e.webp 234w," sizes="100vw" width="1024" height="682"></p><p><span>By improving sample handling, reducing waste, and supporting automation, micro inserts can significantly enhance overall HPLC performance.</span></p><h3><span><strong>Selecting the Best Material: Glass or Plastic?</strong></span></h3><p><span>The choice of insert material can influence both analytical quality and operational costs. Selecting the appropriate insert depends largely on sample composition and solvent compatibility.</span></p><h4><span><strong>Glass Inserts</strong></span></h4><p><span>Glass inserts are often preferred for demanding analytical applications.</span></p><p><span>Their advantages include:</span></p><ul><li><span>Excellent chemical resistance</span></li><li><span>Compatibility with organic solvents</span></li><li><span>Minimal risk of analyte interaction</span></li><li><span>Reduced contamination potential</span></li></ul><p><span>For sensitive analyses and reactive samples, glass inserts typically provide the highest level of confidence in sample integrity.</span></p><h4><span><strong>Plastic Inserts</strong></span></h4><p><span>Plastic inserts offer a practical and economical alternative.</span></p><p><span>Advantages include:</span></p><ul><li><span>Lower cost</span></li><li><span>High durability</span></li><li><span>Reduced breakage risk</span></li></ul><p><span>However, certain solvents may interact with plastic materials, making them less suitable for some applications involving aggressive chemical environments.</span></p><h3><span><strong>Common Micro Insert Designs and Their Applications</strong></span></h3><p><span>Different insert geometries are available to accommodate specific analytical requirements.</span></p><h4><span><strong>Flat-Bottom Inserts</strong></span></h4><p><span>Flat-bottom inserts represent the most versatile option and are widely used for routine analyses.</span></p><p><span>Key benefits include:</span></p><ul><li><span>Excellent sample accessibility</span></li><li><span>High sample recovery</span></li><li><span>Broad autosampler compatibility</span></li></ul><p><span>These inserts are suitable for most standard HPLC workflows.</span></p><h4><span><strong>Conical Inserts</strong></span></h4><p><span>Conical inserts feature a tapered bottom that concentrates the sample into a very small area.</span></p><p><span>This design is particularly advantageous when:</span></p><ul><li><span>Sample volumes are extremely limited</span></li><li><span>Maximum recovery is required</span></li><li><span>Trace-level analysis is being performed</span></li></ul><p><span>The conical geometry helps ensure that even minute sample quantities remain accessible to the autosampler needle.</span></p><h4><span><strong>Spring-Fitted Inserts</strong></span></h4><p><span>Spring-fitted inserts incorporate a plastic spring mechanism that secures the insert within the vial.</span></p><p><span>Advantages include:</span></p><ul><li><span>Stable positioning</span></li><li><span>Reduced insert movement</span></li><li><span>Improved performance in automated systems</span></li></ul><p><span>These inserts are especially useful in laboratories operating high-throughput autosampler platforms.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_3_Flat_Bottom_Insert_left_Conical_Insert_center_Spring_fitted_Insert_right_682322556f.jpg" alt="KNAUER: Fig. 1–3  Flat Bottom Insert (left), Conical Insert (center), Spring-fitted Insert (right)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_3_Flat_Bottom_Insert_left_Conical_Insert_center_Spring_fitted_Insert_right_682322556f.jpg 245w," sizes="100vw" width="1152" height="366"></p><p><span>Selecting the appropriate insert design can improve recovery, reduce variability, and enhance overall analytical reliability.</span></p><h3><span><strong>Applications Where Small Volumes Matter Most</strong></span></h3><p><span>Micro inserts are widely used in analytical fields where sample quantities are limited and measurement accuracy is critical.</span></p><h4><span><strong>Pharmaceutical Analysis</strong></span></h4><p><span>Drug development and quality control frequently involve precious samples and low-concentration impurities.</span></p><p><span>Micro inserts help maximize recovery and ensure accurate quantification, supporting reliable pharmaceutical testing and regulatory compliance.</span></p><h4><span><strong>Environmental Analysis</strong></span></h4><p><span>Environmental laboratories often work with trace contaminants present at extremely low concentrations.</span></p><p><span>Micro inserts improve sensitivity and help preserve valuable extracts, making them useful for pollutant monitoring and environmental investigations.</span></p><h4><span><strong>Forensic and Toxicological Testing</strong></span></h4><p><span>In forensic science, sample quantities are frequently limited and cannot be replaced.</span></p><p><span>Micro inserts enable efficient analysis of small biological specimens while preserving as much material as possible for potential repeat testing.</span></p><p><span>Across these disciplines, the ability to obtain high-quality results from minimal sample volumes makes micro inserts an indispensable analytical tool.</span></p><h3><span><strong>Supporting Sustainable Laboratory Practices</strong></span></h3><p><span>Sustainability has become an increasingly important consideration in analytical laboratories.</span></p><p><span>Micro inserts contribute to greener workflows by:</span></p><ul><li><span>Reducing sample consumption</span></li><li><span>Lowering solvent usage</span></li><li><span>Minimizing reagent waste</span></li><li><span>Improving overall analytical efficiency</span></li></ul><p><span>These benefits align well with laboratory initiatives aimed at reducing environmental impact and operating costs.</span></p><p><span>Future developments may introduce inserts manufactured from recyclable or biodegradable materials, further enhancing their sustainability profile. Even today, however, micro inserts represent a straightforward way to improve resource efficiency without compromising analytical performance.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_sustainability_cd6da6aab0.webp" alt="KNAUER / unsplash.com: Sustainability" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_sustainability_cd6da6aab0.webp 245w," sizes="100vw" width="720" height="412"></p><h3><span><strong>Small Components, Significant Benefits</strong></span></h3><p><span>Although micro inserts occupy only a small space inside an HPLC vial, their influence on analytical quality can be substantial.</span></p><p><span>By improving sample recovery, enhancing sensitivity, minimizing evaporation, reducing carryover, and supporting automated workflows, these simple accessories help laboratories achieve more reliable and reproducible results. Careful selection of insert material and design allows analysts to tailor performance to specific applications while making better use of valuable samples.</span></p><p><span>Micro inserts serve as a reminder that in chromatography, attention to the smallest details often delivers the greatest improvements in analytical success.</span></p>

Why Micro Inserts Are Essential for Precision in HPLC Analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 15th June 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies, Shimadzu </strong>and<strong> Thermo Fisher Scientific, </strong>technical note by<strong> KNAUER </strong>and brochure by <strong>Waters Corporation!</strong></p><h3><strong>1. Agilent Technologies: Large-Volume Dilute-and-Shoot Analysis of PFAS in Drinking Water Using the Altura PFAS Column</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_analysis_pfas_drinking_water_altura_5994_9186en_agilent_a66334e5b0.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Per‑ and polyfluoroalkyl substances (PFAS) are synthetic fluorinated compounds valued for their oil‑ and water‑repellent properties, but their environmental persistence and mobility have driven the development of increasingly stringent regulations for drinking water monitoring. Existing dilute-and-shoot methods, such as EPA 8327 and ASTM D7979, were originally designed for screening PFAS in nonpotable water. These methods typically rely on 10 to 30&nbsp;µL injection volumes, setting practical quantitation limits appropriately 10 ng/L.<sup>1,2&nbsp;</sup></p><p>We present a large‑volume injection workflow that extends the capabilities of these methods toward trace PFAS quantitation in drinking water. Using the Agilent 6495D triple quadrupole LC/MS (LC/TQ) coupled with a 1290 Infinity II LC, we achieved injections of 200 µL of methanol‑diluted water on the Altura PFAS column without requiring SPE concentration. The Altura PFAS column maintained peak symmetry and retention stability under these injection conditions. This configuration enabled a working calibration range of 0.25 to 25 ng/L, extending the low-end sensitivity substantially relative to published dilute-and shoot methods.<sup>1,2&nbsp;</sup></p><p>This performance enhancement simplifies operations by removing labor‑intensive extraction steps, while maintaining sensitivity required for regulatory guidelines thereby supporting high‑throughput PFAS workflows suitable for routine drinking water analysis. The PFAS compounds analyzed were PFBS, HFPO-DA, PFHpA, PFHxA, PFHxS, PFOA, PFOS, PFNA, and PFDA. These compounds were selected due to their relevance to ongoing regulatory actions and proposed maximum contaminant level (MCL) considerations for drinking water.&nbsp;</p><p>Specific highlights of this experiment include:&nbsp;</p><ul><li>Minimized peak tailing and solvent effects with 200 µL injections water/methanol (1:1) injections.</li><li>Increased throughput by elimination of solid phase extraction (SPE).&nbsp;</li><li>Enhanced sensitivity of 6495D LC/TQ at low end (0.25&nbsp;ng/L) for the dilute-and-shoot method in drinking&nbsp;water.</li></ul><h4><strong>Experimental&nbsp;</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=+1290+Infinity+II"><strong>Agilent 1290 Infinity II LC</strong></a> coupled to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D"><strong>Agilent 6495D LC/TQ system</strong></a> was utilized for the LC/MS/MS analysis. An Infinity&nbsp;II multisampler with an extended multidraw capillary tube (G7137-68307) was connected to the needle seat and injection valve with a union for large volume injection (200&nbsp;µL). The data were acquired with MassHunter acquisition software version 12.2. All LC/MS parameters are shown in Table 1 and 2. PFAS multiple reaction monitoring (MRM) transition methods were imported from the PFAS MRM database (G1736AA) while labelled homologues were optimized to obtain correct MRM transitions (Table 3).</p><h4><strong>Conclusion&nbsp;</strong></h4><p>This study demonstrates a practical large‑volume dilute-and-shoot workflow for the determination of PFAS in drinking water using the Agilent 1290 Infinity II LC coupled to the 6495D triple quadrupole LC/MS, and Agilent Altura Poroshell PFAS column. With 200 µL water samples diluted in methanol can be injected directly, eliminating the need for SPE concentration and significantly reducing sample preparation time. This method exhibits good sensitivity, accuracy, and reproducibility. The Altura PFAS column maintained consistent peak performance and retention stability even at low sub‑ppt concentrations, supporting long‑term reliability for trace‑level PFAS monitoring. Overall, the findings highlight the method potential to support routine, high‑sensitivity PFAS monitoring in drinking water laboratories.</p><h3><strong>2. KNAUER: Size matters - How to perform narrow calibration using the ClarityChrom® GPC Extension</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0049_knauer_size_matters_how_to_perform_narrow_calibration_using_the_claritychrom_gpc_extension_3cfdc7a45b.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a separation technique that separates molecules based on their hydrodynamic size rather than chemical interactions. In a GPC column, separation is based on the ability of molecules to access the pores of the stationary phase. Smaller molecules can penetrate these pores and therefore spend more time inside the column, resulting in a delayed elution. Larger molecules, on the other hand, cannot enter the pores and pass through the column more quickly, leading to earlier elution.&nbsp;</p><p>As a result, molecules are separated by size in solution, providing the basis for the determination of molecular weight and molecular weight distributions. Calibration is therefore a fundamental requirement for ensuring the accuracy and reliability of the results. The ClarityChrom® GPC Extension enables users to efficiently create and apply different calibration models for precise analysis. The workflow presented here focuses on narrow calibration using standards with defined molecular weights and low polydispersity (PD ≈ 1).</p><h4><strong>Material and Methods</strong></h4><ul><li><strong>Software&nbsp;</strong><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=503&amp;search=ClarityChrom"><strong>ClarityChrom</strong></a>® 10.1 - station single instrument license one time base</li><li>ClarityChrom® 10.1 - GPC license for GPC data processing &nbsp;</li></ul></li><li><strong>Pump: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2372"><strong>AZURA P 6.1L isocratic HPLC pump</strong></a> with 10 ml pump head for normal phase applications, stainless steel&nbsp;</li><li><strong>Degasser: </strong>Analytical 2 channel GPC degasser&nbsp;</li><li><strong>Autosampler:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2375"><strong>AZURA® AS 6.1L</strong></a>, analytical HPLC autosampler, 862 bar&nbsp;</li><li><strong>Detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2429"><strong>AZURA® RID 2.1L</strong></a>, analytical refractive index detector&nbsp;</li><li><strong>Thermostat: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2376"><strong>AZURA® CT 2.1</strong></a> ATC00 Capillaries Start-Up Kit with flexible, precut capillaries for analytical HPLC systems with 1/16" connections</li></ul><h4><strong>Conclusion</strong></h4><p>This technical note demonstrates how to successfully create and apply a narrow calibration using the ClarityChrom® GPC Extension. By following the outlined workflow, users can ensure reliable calibration and determination of molecular weight and molecular weight distributions. The described approach supports consistent and reproducible results, making it a valuable tool for routine GPC/SEC analysis. For further optimization and advanced applications, additional related technical and application notes can be consulted.</p><h3><strong>3. Shimadzu: UHPLC Method for Sensitive Automatic Analysis of Thirty-Seven D/L-Amino Acids and for Liquor Profiling</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01169_en_2dab331398.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Enables high-sensitivity simultaneous fast analysis of thirty-seven D/L-amino acids using simple operations.</li><li>Good reproducibility of analytical results can be achieved by keeping the derivatization reaction time constant.</li><li>Statistical analysis software enables easy multivariate analysis of multiple samples.</li></ul><p>Recent advances in analytical techniques have revealed the presence of D-amino acids in fermented foods and biological samples and clarified their role in the taste, preservation, and aroma of foods , and as potential disease biomarkers. Therefore, the demand for D/L separation of amino acids is increasing. Because D-amino acids are only present in trace amounts in fermented foods and biological systems, unlike L-amino acids, it is necessary to determine the concentration of each D-amino acid by separating them from the high concentrations of Lamino acids.&nbsp;</p><p>A comprehensive method for quantitating the thirty-seven D/Lamino acids was previously reported1), in which two chiral derivatizing reagents were employed to provide different diastereomers for each amino acid, with the instrument automatically switching between two separation methods to analyze each sample twice.&nbsp;</p><p>This article introduces an improved method that employs a single chiral derivatizing reagent to enable simultaneous analysis of D/L-amino acidsin a shorter time.</p><h4><strong>D/L-Amino Acid Analysis by UHPLC&nbsp;</strong></h4><p>In general, LC/MS or multi-dimensional LC is used to analyze D/L-amino acids by HPLC, because it is difficult to separate proteinogenic D/L-amino acids with a single separation mode. However, it is known that LC/MS is susceptible to matrix effects and less quantitative than HPLC. It is also known that the multidimensional LC method requires a long analysis time and very complicated HPLC setup. Therefore, a simple operational method that provides good separation for D/L-amino acids in a short time is needed.&nbsp;</p><p>For food analysis, a small particle column is used to achieve appropriate separation of the small amounts of D-amino acids from the large amounts of L-amino acids and co-existing contaminants. A <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=X3"><strong>UHPLC system (Nexera X3</strong></a>) was employed in this study due to the increased system pressure caused by the use of a small particle column.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>A method was developed for simultaneous separation of OPA/NIBC diastereomers for thirty-seven D/L-amino acids using a simple UHPLC system in approximately half the time of the conventional methods. By optimizing the derivatization reaction, all compounds were determined sensitively, accurately, and precisely. Separation of the target compounds from the contaminants was good in the liquor samples when evaluated using real liquor samples. It is expected to be easily applicable to D/L-amino acid profiling in food and beverage samples without using expensive MS detection or complicated multidimensional HPLC configurations as long as samples are prepared properly. Moreover, D/L-amino acid profiling could be used to research and develop foods containing D-amino acids intended to help to maintain and improve the intestinal environment, and could help in the early detection of diseases and determining optimal treatment strategies through biomarker discovery</p><h3><strong>4. Thermo Fisher Scientific: Screening of per- and polyfluoroalkyl substances (PFAS) in cosmetics: Utilizing a new combustion-ion chromatography system for total organic fluorine (TOF) analysis</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ap_004098_ic_systems_combustion_ic_environmental_ap004098_na_en_a3a1d33a24.pdf"><strong>Full PDF for download</strong></a></li></ul><p>This application note describes a method for <strong>screening per- and polyfluoroalkyl substances (PFAS) in textiles</strong> using total organic fluorine (TOF) analysis by combustion-ion chromatography (C-IC). PFAS are widely used in textiles to provide water, oil, and stain resistance, but increasing concerns about their environmental persistence and potential health effects have led to stricter regulations worldwide. California’s AB 1817 legislation, for example, limits TOF concentrations in textiles to 100 ppm beginning in 2025 and 50 ppm by 2027, creating a need for reliable analytical methods capable of assessing PFAS content in textile products.</p><p>The study evaluates a new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2150"><strong>Thermo Scientific Cindion Combustion Ion Chromatography System</strong></a>, which combines a Dionex Inuvion IC system with a Cindion combustion/absorption module. The system features an optimized Z-fold combustion tube design that improves combustion efficiency, shortens analysis time, reduces instrument footprint, and allows complete control through Chromeleon software. Unlike surface-sensitive techniques such as PIGE, the combustion-IC approach analyzes the entire sample, providing results that are independent of sample thickness. The method measures total fluorine (TF) after combustion and total inorganic fluorine (TIF) by direct IC analysis, with TOF calculated as the difference between TF and TIF.</p><p>To validate the approach, six textile samples representing waterproof and stain-resistant consumer products were analyzed and compared with results previously obtained using a Nittoseiko combustion-IC system. TOF concentrations ranged from approximately 1 ppm in one nylon fabric to nearly 375 ppm in a polyester baby bib. The values generated by the new Cindion system showed excellent agreement with the reference system, achieving between 100% and 105% of the previously reported results. Reproducibility was also strong, with relative standard deviations below 8% for all samples.</p><p>The authors conclude that the new Cindion C-IC platform provides an accurate, reliable, and efficient solution for PFAS screening in textiles. In addition to matching the performance of the established combustion-IC method, the enhanced system offers a flexible “2-in-1” configuration that allows seamless switching between combustion-IC and standalone IC operation. This improves workflow efficiency, reduces manual intervention, and provides manufacturers and testing laboratories with a practical tool for monitoring compliance with evolving PFAS regulations in textile products.</p><h3><strong>5. Waters Corporation: Advanced Acquisition Modes for the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2433"><strong>Xevo MRT P10 Mass Spectrometer</strong></a></h3><ul><li>Brochure</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009362_9baa39e7de.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The Waters solution sheet introduces the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2433"><strong>Xevo MRT P10 Mass Spectrometer</strong>,</a> a next-generation high-resolution mass spectrometer designed to provide significantly greater analytical depth, sensitivity, and flexibility for modern omics workflows. Compared with the previous Xevo MRT platform, the instrument delivers a reported <strong>20-fold increase in MS/MS sensitivity</strong>, incorporates Wideband Enhanced Duty Cycle (WB EDC) technology, and supports ultrafast acquisition rates of up to <strong>100 Hz for MS data</strong> and <strong>200 Hz for MS/MS data</strong>. These performance improvements are intended to enhance the detection of low-abundance compounds while maintaining high-quality spectral information across complex biological samples.</p><p>A major focus of the document is the introduction of the new <strong>SONAR Pulse Acquisition Mode</strong>, an advanced data-independent acquisition (DIA) approach. SONAR Pulse improves selectivity by rapidly stepping a quadrupole isolation window across a defined precursor mass range, allowing fragment ions to be associated with narrower precursor windows than in conventional DIA experiments. This strategy reduces spectral complexity, improves confidence in compound identification, and increases the likelihood of detecting low-level analytes that might be missed by traditional data-dependent acquisition (DDA) methods. The method supports precursor and product ion scans up to m/z 2000, multiple quadrupole isolation window sizes, acquisition speeds up to 200 Hz, and full compatibility with WB EDC technology.</p><p>The second major innovation described is <strong>Mixed Mode Acquisition</strong>, a hybrid workflow that combines targeted and untargeted analysis within a single injection. In this approach, high-sensitivity <strong>Tof MRM</strong> quantification is performed simultaneously with untargeted DIA (MSE) acquisition. This enables users to obtain quantitative data for predefined targets while also collecting comprehensive full-scan information for discovery and screening purposes. The workflow integrates seamlessly with Waters software tools, allowing targeted quantification through the MS Quan application and untargeted analysis through UNIFI or export to open data formats such as mzML for processing in third-party software packages including MZmine, MS-DIAL, DIA-NN, PEAKS, and Progenesis QI.</p><p>According to Waters, both acquisition strategies are designed to maximize information obtained from each sample injection. SONAR Pulse emphasizes deeper molecular coverage, improved selectivity, and simplified interpretation of complex DIA datasets, while Mixed Mode provides a unified quantitative–qualitative workflow that combines targeted measurement and discovery screening without compromising sensitivity or acquisition speed. Together, these capabilities position the Xevo MRT P10 as a versatile platform for metabolomics, lipidomics, proteomics, and other multiomics applications where both comprehensive characterization and accurate quantification are required.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 25, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Metrohm

Agilent Technologies

Technology Networks

Unchained Labs

Thermo Fisher Scientific

SelectScience

Evosep Biosystems

Waters Corporation

<p><strong>The rapid growth of genetic medicines - including oligonucleotides, plasmid DNA, mRNA, lipid nanoparticles (LNPs), and viral vectors - has introduced unprecedented analytical challenges. From shear‑induced degradation to poor resolution and reproducibility, traditional SEC approaches often struggle to deliver actionable insights for development, scale‑up, and QC.</strong></p><p>In this webinar, we explore next‑generation SEC columns designed specifically for mega molecules. Discover how wide‑pore SEC technology enables robust, high‑resolution size‑based characterization while preserving sample integrity. Through real‑world examples and best practices, our speaker will demonstrate how modern SEC workflows support faster decision‑making, improved inter‑lab reproducibility, and smoother regulatory pathways for advanced genetic medicine products.</p><h4><strong>What You’ll Learn</strong></h4><h5><strong>Attendees will gain practical insights into:</strong></h5><ul><li>Common SEC challenges in analyzing plasmids, mRNA, LNPs, and viral vectors</li><li>Strategies to minimize shear degradation and maintain biomolecule integrity during separation</li><li>Optimizing SEC methods for mega molecules using wide‑pore column technology</li><li>How to achieve reproducible, high‑resolution separations across labs and sites</li><li>Best practices for method development and extending SEC column lifetime</li><li>How faster SEC workflows can reduce development timelines and support regulatory submissions</li></ul><h4><strong>Why attend?</strong></h4><p>Traditional sizing techniques often fall short when applied to today’s complex, high‑molecular‑weight genetic medicines, leading to low resolution, high variability, and long run times.</p><h5><strong>In this session, you’ll learn how modern wide‑pore SEC solutions:</strong></h5><ul><li>Deliver robust, high‑throughput profiling for early discovery through late‑stage development</li><li>Support multi‑attribute characterization, including size distribution, aggregates, and integrity</li><li>Enable seamless integration with MALS detection for enhanced molecular weight and structural insight</li></ul><h4><strong>Who should attend?</strong></h4><p>This webinar is ideal for analytical scientists, process development teams, QC laboratories, and CROs working in cell and gene therapy, vaccines, and RNA‑based therapeutics.</p><blockquote><p><strong>Presenter: </strong>Ali Tiss, Ph.D., MSc., Eng. (Chemistry Market Development Manager, Biopharma &amp; Cell and Gene Therapies, Waters Corporation)</p><p>Ali joined Waters in 2021 and has since supported colleagues and users in the biomolecule separation field (proteins, peptides, Abs, conjugates, Cell &amp; Gene Therapy products). Ali completed his Ph.D. in biochemistry and structural biology at the Mediterranean University (CNRS, Marseille, France), where he contributed to the R&amp;D of the anti-obesity drug, Xenical®, developed by Roche Ltd. Then, he earned over two decades of research experience in biochemistry, liquid chromatography, mass spectrometry, and proteomics, focusing on detecting and characterizing biomarkers related to human diseases such as cancer, diabetes, and obesity. Ali is passionate about supporting customer business and innovative applications.</p></blockquote>

Advancing Size Exclusion Chromatography (SEC) for Characterization of Genetic Therapeutics

Advance SEC workflows for genetic medicines. Discover wide-pore column technology for high-resolution characterization of mRNA, LNPs, and viral vectors

Advancing SEC for Characterization of Genetic Medicines

<div><p><strong>As biomarker testing becomes central to precision oncology workflows, research laboratories are under increasing pressure to select and integrate methods that deliver reliable results across diverse sample types, including liquid biopsy.</strong><br>This webinar explores how to make informed methodological decisions when implementing and combining approaches for biomarker detection.<br>Through real-world examples, attendees will gain insight into balancing sensitivity, turnaround time, and sample constraints, alongside key considerations for verification and workflow design. Join to better align your research laboratory strategy with evolving clinical and translational demands.</p><h5><strong>Attend this webinar to:</strong></h5><ul><li>Gain an overview of NGS, qPCR and dPCR targeted for biomarker detection across FFPE, cytology, and liquid biopsy samples</li><li>Learn to integrate multiple methodologies for optimal results, including key considerations like limitations, workflows, and turnaround time</li><li>Discover best practices for analytical validation to help ensure reliable implementation in research laboratories</li></ul><p>“For Research Use Only. Not for use in diagnostic procedures.”</p><blockquote><p><strong>Presenter:</strong> Javier Hernández Losa, PhD ( Director, Molecular Biology Laboratory, University Hospital Vall d’Hebron )</p><p>Dr. Javier Hernández Losa has a PhD in biology from the Universidad Autonoma de Madrid (2005). He has led the molecular biology laboratory within the pathology service of the Hospital Universitari Vall d´Hebron in Barcelona since 2006. The laboratory specializes in the study of biomarkers, mainly in tissue samples, and these studies have recently been extended to liquid biopsy samples. Over the last few years, he has coordinated three PhD theses and supervises several master’s projects based on the study of cfDNA and exosomes, both in in vitro models and in clinical samples from patients with advanced tumors. Currently, he is a member of the Low Prevalence Tumors Program of CIBERONC and is also the principal investigator of several research projects.</p></blockquote></div>

Technology Networks: Selecting Biomarker Methods for Evolving Oncology Research Workflows

Selecting Biomarker Methods for Evolving Oncology Research Workflows

Optimize biomarker detection in oncology research. Learn to balance NGS, qPCR, and dPCR workflows across FFPE, cytology, and liquid biopsy samples

Selecting Biomarker Methods for Oncology Research

<h4><strong>How can you know that you catch all PFAS?</strong></h4><p><strong>Per- and polyfluoroalkyl substances (PFAS) comprise a group of highly persistent compounds that pose significant environmental concerns, particularly in water quality monitoring. Comprehensive characterization of PFAS contamination remains analytically challenging due to the chemical diversity of these substances and the limitations of individual methods.</strong></p><p>This webinar discusses complementary ion chromatography–based approaches for PFAS analysis in accordance with current standards. Combustion ion chromatography (CIC) enables the reliable determination of the total PFAS-related sum parameter AOF (adsorbable organically bound fluorine) and is standardized in DIN 38409-59, ISO 18127, and EPA 1621. &nbsp;This approach provides a robust overview of PFAS contamination levels.</p><p>However, the AOF approach does not capture all PFAS. Ultra-short chain PFAS are highly hydrophilic and are less efficiently retained on activated carbon, and therefore are not detected by AOF analysis. One of the most prominent ultra-short chain PFAS is trifluoroacetic acid (TFA), a relevant degradation product that may account for a significant proportion of total PFAS in water samples. Ion chromatography coupled with mass spectrometry (IC-MS/MS) provides a sensitive and reliable approach for TFA determination in water matrices. In this webinar, the validated approach in accordance with DIN 38407-53 will be presented.&nbsp;</p><h4><strong>Key learnings of this webinar:</strong></h4><ul><li>Understand the regulatory background for PFAS analysis in water (DIN 38409‑59, ISO 18127, EPA 1621, DIN 38407‑53)</li><li>Learn how combustion ion chromatography (CIC) enables the reliable determination of total PFAS-related parameters such as AOF</li><li>Understand the relevance of trifluoroacetic acid (TFA) as a key ultra-short chain PFAS in water quality monitoring</li><li>Discover how IC‑MS/MS provides a sensitive and reliable approach for TFA determination according to DIN 38407‑53</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Water utility operators who need robust and dependable analytical techniques to guarantee the safety of drinking water</li><li>Scientists, researchers, and analytical method developers who need to stay up -to date on sophisticated/state-of-the-art methods for water analysis</li><li>Laboratory staff and managers who considering implementing reliable, cost- and time-efficient analysis of HAA in their lab</li></ul><blockquote><p><strong>Speaker: </strong>Dr. Katinka Ruth (Senior Application Specialist IC, Metrohm International Headquarters)</p><p>Dr. Ruth received her MSc in Chemistry at the Technical University Munich and a PhD in Environmental Sciences at the Swiss Federal Institute of Technology (ETH Zurich). She gained extensive knowledge in ion chromatography and hyphenation of ion chromatography to mass spectrometers during her 17 years of experience at Metrohm International Headquarters, where she holds a position as Senior Application Specialist.</p></blockquote><blockquote><p><strong>Speaker:&nbsp;</strong>Theresa Steurer (Product Specialist IC, Metrohm International Headquarters)</p><p>Theresa Steurer is a Product Specialist in the Ion Chromatography Competence Center at Metrohm International Headquarters in Herisau, Switzerland. She holds a degree in chemistry from the University of Vienna.</p><p>With over eight years of experience in the ion chromatography applications laboratory, Theresa has developed extensive expertise in ion chromatography, with a particular focus on combustion ion chromatography.</p></blockquote>

Metrohm: PFAS Analysis with Ion Chromatography-Based Techniques

From Challenge to Confidence: PFAS Analysis with Ion Chromatography-Based Techniques

Advance your PFAS water monitoring. Combine Combustion IC for total AOF determination with IC-MS/MS for sensitive ultra-short chain TFA quantification

How to Ensure Complete PFAS Detection in Water Analysis

<p><strong>Robustness, reproducibility, and scalability are becoming increasingly important as proteomics workflows move closer to clinical and diagnostic applications.</strong></p><p>In this webinar, we will explore how the Evosep Eno supports the development of robust diagnostic assays through standardized, high-throughput LC–MS workflows. Join us to learn how reproducible performance, streamlined operation, and scalable proteomics methods can help accelerate the transition from discovery research to real-world applications.</p><h5><strong>Robust Data from Evosep Eno coupled to Exploris 480 for the Development of Diagnostic Assays</strong></h5><p>Models derived from machine learning approaches possess enormous diagnostic potential. At OXcan we create reliable machine learning models using training data sets generated from LC-MS data. A good model is accurate, reliable, and resistant to overfitting. In order to achieve this performance, the clinical and control data must have enough features and be sufficiently robust to allow the model to generate diagnostic features with a high degree of confidence.</p><p>Accepting that there is a pressing need to create reliable proteomic data, to facilitate the training of machine learning models, we will discuss our workflow for generating robust data from digested plasma samples. The workflow leverages the performance capabilities of our Evosep Eno LC system coupled to a Thermo Exploris MS. This LC-MS platform gives us reproducible data, with the throughput required for the development of our machine learning derived diagnostic assays.</p><blockquote><p><strong>Speaker:</strong> Giles Drinkwater (Researcher at OXcan Analytics)</p></blockquote>

Evosep: Assay Development for translational and research applications

Assay Development for translational and research applications

Advance translational proteomics for diagnostic assays. Explore high-throughput LC-MS workflows using Evosep Eno and Exploris 480 for machine learning models

High-Throughput LC-MS Workflows for Diagnostic Assays

<p><strong>Effective gene therapies require a thorough understanding of your delivery vehicle. Is it the right size? Does it contain an intact capsid, payload, and surface markers? Classic characterization methods don’t give structural insights, only look for a single component, and require purified samples to get actionable results. With the rise of in vivo CAR-T cell therapies, viral vectors have become increasingly complex to target the right cells and deliver cargo at the right time. Researchers need flexibility to adapt their characterization to the rapidly changing strategies for specified cell targeting and efficient gene delivery.</strong></p><p>Leprechaun is the only platform to deliver the complete biophysical ins and outs of individual lentivirus particles, with any combination of surface markers, at any stage from cell harvest to final stage, purified. Leprechaun combines interferometry and multi-channel fluorescence using a maximum of 25 µL of lentivirus, to characterize up to 16 samples in as little as a few hours. By assessing quality of lenti preps earlier in development, Leprechaun helps researchers obtain the most structurally intact lentivirus.</p><p>In this webinar, we'll cover how Leprechaun delivers both sizing and structural data on lentivirus particles throughout purification and pinpoint steps for optimization. We’ll show how Leprechaun goes beyond ELISA and qPCR to provide additional insights by measuring multiple parameters on the same sample. Additionally, we’ll show some unique assays to measure breakout CAR expression to make sure your lenti is ready for in vivo CAR-T action. All these results showcase how Leprechaun is the go-to tool for complete biophysical characterization of lenti being developed for in vivo CAR-T cell therapies.<br>&nbsp;</p><blockquote><p><strong>Presenter:&nbsp;</strong>Brian Haldeman<strong> </strong>(Product Manager, Leprechaun, Unchained Labs)</p></blockquote>

Unchained Labs: Know the ins and outs of your lentivirus with Leprechaun 

Know the ins and outs of your lentivirus with Leprechaun

Characterize lentivirus particles for in vivo CAR-T cell therapies. Learn how Leprechaun delivers biophysical insights on sizing and surface markers

Advanced Lentivirus Characterization with Leprechaun

<p><strong>Advancing protein and viral vector characterization requires detection strategies that deliver higher sensitivity without adding complexity to established workflows. Traditional UV detection can introduce challenges such as labelling steps, baseline drift, and lower signal‑to‑noise ratios, making it harder to resolve critical quality attributes in capillary electrophoresis–sodium dodecyl sulfate (CE‑SDS) as workflows scale and regulatory expectations increase.</strong></p><p>In this SelectScience® TechTalk, Staff Application Scientist, Peter Holper, with 15+ years of biopharma industry experience, will demonstrate how native fluorescence detection (NFD) on the BioPhase 8800 system is raising the bar for CE-SDS analysis. By harnessing the natural fluorescence of aromatic amino acids, NFD eliminates protein labeling while delivering improved signal-to-noise ratios and cleaner baselines.</p><p>You will also discover how 8‑capillary parallel processing accelerates throughput across workflows such as CE‑SDS purity analysis and AAV capsid protein characterization, supported by real data showing accuracy and repeatability across detection modes. With streamlined sample preparation and stable baselines, your lab can get to answers faster and with greater confidence.</p><h5><strong>Certificate of attendance</strong></h5><p>If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p><p>If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.</p><h5><strong>TechTalk details</strong></h5><ul><li><strong>Cost: Free to attend</strong></li><li><strong>Location: Online</strong></li><li><strong>Duration: 20 minutes</strong></li></ul><p><i>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.</i></p><h5><strong>This event is perfect for:</strong></h5><ul><li>Analytical development scientists</li><li>Process development scientists</li><li>CMC Directors</li></ul><blockquote><p><strong>Speaker:</strong> Peter Holper (Staff Application Scientist, SCIEX)</p><p>Peter Holper earned his degree in Biochemistry and Cell Biology from the University of California, San Diego. He has over 15 years of experience in biopharma, including as an analytical chemist at Eli Lilly and Company where he was responsible for developing the analytical control strategy for bioproducts. Holper currently works at SCIEX where he is responsible for developing and optimizing CE applications and providing customer demo sample analysis support.</p></blockquote>

SelectScience: Native fluorescence detection for high-throughput CE-SDS and AAV characterization in drug development SCIEX

Native fluorescence detection for high-throughput CE-SDS and AAV characterization in drug development

Discover native fluorescence detection (NFD) on the BioPhase 8800 system for label-free, high-throughput CE-SDS purity analysis and AAV characterization

Native Fluorescence for CE-SDS and AAV Characterization

<p><strong>On June 23, Abbie Breton, Applications Development Manager, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. Yuliya McAnany, Applications Scientist – Process Resins at Tosoh Bioscience, will showcase a suite of high-performance tools for reliable separation and analysis of AAV particles. Yuliya will start by discussing Tosoh’s novel full capsid enrichment method for TOYOPEARL GigaCap Q-650S resin combined with choline chloride, achieving 90% purity and 75% recovery. The conversation will include a look into Tosoh’s analytical toolkit for increased reliability, and improved sensitivity for critical quality monitoring.</strong></p><p>Participants will learn how to simplify the development of a high-performing enrichment step and will learn how Tosoh’s TSKgel Q-STAT, TSKgel GMPWxl, and LenS3 MALS detector can be used to advance quality monitoring of AAV products.</p><p>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</p><blockquote><p><strong>Presenter:</strong> Abbie Breton (Applications Development Manager, Tosoh Bioscience)</p></blockquote><blockquote><p><strong>Presenter:</strong> Yuliya McAnany (Applications Scientist – Process Resins, Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit

Tosoh Tuesday - Exploring the AAV Toolkit

Advance your AAV quality monitoring. Explore Tosoh's full capsid enrichment methods using TOYOPEARL resins and precise TSKgel SEC analysis with MALS detection

Exploring the AAV Toolkit: Tosoh Chromatography Webinar

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>GLP-1 receptor agonists are among the most impactful peptide therapeutics, but their structural complexity presents significant analytical challenges. From sequence modifications to lipidation and manufacturing variability, achieving confident characterization requires advanced approaches.</strong></p><p>Join us to learn how a streamlined LC-MS workflow can simplify GLP-1 analysis. This session will highlight integrated strategies combining UHPLC separation, high-resolution mass spectrometry, and automated data analysis to support robust characterization and impurity monitoring of peptides such as semaglutide and tirzepatide.</p><h5><strong>What You Will Learn</strong></h5><ul><li>How to optimize LC-MS workflows for comprehensive GLP-1 peptide characterization</li><li>Practical strategies for improving separation of closely related peptide species</li><li>Approaches to enhance sequence coverage using advanced fragmentation techniques</li><li>Methods for confident impurity profiling and structural heterogeneity assessment</li></ul><p>Secure your spot today and gain practical insights to simplify complex GLP-1 analysis.</p><blockquote><p><strong>Presenter: </strong>Dr. Sara Carillo (Bioanalytical Research Lead, NIBRT)</p><p>Dr. Carillo specializes in structural characterization of complex biomolecules using mass spectrometry and NMR. At NIBRT, she develops advanced analytical strategies to better understand biomanufacturing processes and the structural complexity of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Presenter: </strong>Dr. Kai Sheffler (Senior Manager, Product Manager for BioPharma Workflows, Thermo Fisher Scientific)</p><p>Dr. Scheffler is a biochemist specializing in LC-MS technologies for biopharmaceutical analysis. His expertise includes peptide mapping, intact mass analysis, and impurity profiling using HRAM MS. He focuses on developing LC-MS solutions for comprehensive characterization and quality assessment of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Moderator:</strong> Dr. Kelly Broster (Sr Manager, Market Development &amp;&nbsp;Collaborations, Pharma Biopharma, Thermo Fisher Scientific)</p></blockquote></div></div></div>

Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Optimize LC-MS workflows for GLP-1 analysis. Master impurity profiling, peptide separation, and structural characterization of semaglutide and tirzepatide

Simplifying GLP-1 Agonist Analysis & Impurity MS

<h4><strong>One webinar, two viewing options</strong></h4><p><strong>We know you're busy, select a viewing session that works best for you.</strong></p><h5><br><strong>Viewing Options</strong></h5><p><strong>Session 1</strong></p><ul><li>Tuesday, June 23, 2026</li><li>Los Angeles: 10:00 AM PT | New York: 1:00 PM EST</li><li>Mexico City: 12:00 PM CDT</li></ul><p><strong>Session 2</strong></p><ul><li>Wednesday, June 24, 2026</li><li>London: 9:00 AM BST | Berlin: 10:00 AM CEST&nbsp;</li><li>Mumbai: 1:30 PM IST</li></ul><p>Supported Liquid Extraction (SLE) for the cleanup of biological matrices has several advantages over Solid Phase Extraction (SPE). SLE requires fewer steps and less method development compared to SPE. For most samples that can be processed by liquid-liquid extraction, SLE can usually be used to provide cleaner and faster solvent partitioning. Historically, to get the cleanest SLE extracts, halogenated solvents like dichloromethane were used for elution of target analytes from the sorbent. A supported liquid extraction (SLE) approach that produced clean extracts with high recoveries and low matrix interferences using ethyl acetate, a more sustainable organic solvent, will be presented. Results for steroids, methylmalonic acid (MMA), and underivatized estrogens in human serum, in addition to optimized LC-MS/MS conditions and LC column considerations, are also discussed.</p><h4><strong>Key Learning Points</strong></h4><ul><li>Discover how SLE provides clean extracts for LC-MS/MS quantitation at low concentrations.</li><li>Describe conditions for the extraction and analysis of MMA from human serum.</li><li>Define method parameters for low-level quantitation of estrogens without derivatization.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>All clinical, CROs, and forensic/toxicology analysts using LC/MS-MS.</li></ul><blockquote><p><strong>Presenter: </strong>Stephanie J Marin (Sr. Global Market Development Manager - Applied Markets, Phenomenex)</p><p>Stephanie J. Marin is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University and has expertise in sample preparation, liquid chromatography, and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer-facing roles in customer and technical support, service, and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies, and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA-certified laboratory. She is the author of over 30 peer-reviewed publications, book chapters, and over 100 abstracts presented at national and international meetings.&nbsp;</p></blockquote>

Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods 

Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods

Advance clinical LC-MS/MS analysis. Discover sustainable Supported Liquid Extraction (SLE) methods for steroids, MMA, and underivatized estrogens in serum

Sustainable SLE and LC-MS/MS for Clinical Analysis

<p><strong>Whether you’re starting a new method or running a routine one, having a checklist to review before starting can help avoid chromatography problems.</strong></p><h4><strong>In this webinar, we will discuss:</strong></h4><ul><li>Sample characteristics (target analytes, matrix, etc.) and how to prepare the sample for LC analysis</li><li>Which supplies to use to get the best results possible</li><li>How to select the appropriate column, equilibrate it, benchmark it with your system, and how to take care of it</li><li>Getting to know your LC system, the importance of maintenance and having a “housekeeping” list.</li></ul><blockquote><p><strong>Speaker:</strong> Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)</p><p>Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.</p><p>Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.</p><p>Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.</p></blockquote>

Agilent Technologies: Becoming A Better Chromatographer Webinar Series

Preparing for LC Analysis: What to Consider Ahead of Time

Master LC analysis preparation. Discover essential checklists for sample matrix prep, column benchmarking, and routine liquid chromatography maintenance

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