<p><strong>In the world of biotherapeutics, bigger often means better — at least when it comes to circulation time in the bloodstream. One of the most widely used strategies to extend the half-life of protein drugs is PEGylation: attaching polyethylene glycol (PEG) chains to a therapeutic protein. The added polymer shields the protein, reduces immune clearance, and improves pharmacokinetics.</strong></p><h4><strong>PEGylation creates a major analytical challenge</strong></h4><p>PEG is <strong>large and polydisperse</strong> (a distribution of chain lengths), and when attached to already complex, glycosylated proteins, it creates a molecular puzzle. How many PEGs are attached? Where are they attached? Are there impurities left behind?</p><p>In this study, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Charge Detection Mass Spectrometry and a Glu-C/Lys-C Digestion-Based Data-Dependent Approach Suggest Mono-PEGylation of a Heterogenous Therapeutic Protein</strong></a>, researchers developed a powerful mass spectrometry workflow that answers these questions — using Orbitrap-based charge detection mass spectrometry and a clever bottom-up strategy. The result? A confident determination that the therapeutic protein carries <strong>a single ~30 kDa PEG</strong>, along with precise mapping of its attachment sites.</p><p>Let’s break down how they did it.</p><h3><strong>The analytical problem: pegylation meets glycosylation</strong></h3><p><strong>The therapeutic protein studied here is already highly heterogeneous:</strong></p><ul><li><strong>165 </strong>amino acids</li><li><strong>3 </strong>N-glycosylation sites</li><li><strong>1 </strong>O-glycosylation site</li></ul><p>Add a <strong>~30 kDa polydisperse PEG </strong>on top of that, and conventional mass spectrometry quickly runs into trouble:</p><ul><li>PEG ionizes very efficiently, masking protein charge states</li><li>Charge envelopes overlap</li><li>Intact mass deconvolution becomes difficult</li><li>PEGylated peptides fragment poorly</li></ul><p>Traditional approaches like post-column triethylamine (TEA) addition or MALDI have <strong>limitations</strong>, especially when glycosylation adds another layer of complexity.</p><p>This is where <strong>Orbitrap-based Charge Detection Mass Spectrometry (CD-MS)</strong> changes the game.</p><h3><strong>Step 1: Measuring the intact mass with charge detection MS</strong></h3><p>The <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>team</strong></a><strong> </strong>used a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/560"><strong>Thermo Scientific Q Exactive UHMR</strong></a> instrument operating in <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/direct-mass-technology.html"><strong>Direct Mass Technology mode</strong></a>.</p><p><strong>Unlike conventional ensemble MS, CD-MS measures:</strong></p><ul><li>The m/z of each individual ion</li><li>The charge of each ion directly</li></ul><p>This enables direct mass determination of highly heterogeneous species.</p><h5><strong>What did they see?</strong></h5><p><strong>From the data:</strong></p><ul><li><strong>Non-PEGylated protein (TP):</strong> ~28.95 kDa</li><li><strong>PEGylated protein (PEG-TP):</strong> ~58.42 kDa</li></ul><p>That mass shift strongly supports <strong>mono-PEGylation</strong> — one PEG per protein molecule.</p><p>Even more impressively, <strong>CD-MS revealed:</strong></p><ul><li>A minor dimer impurity (~3.7%)</li><li>A small free PEGylation reagent (~30.4 kDa), likely residual reagent</li></ul><p>The mirror plot (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Figure 2 on page 4</strong></a>) visually highlights this mass shift, clearly separating the TP and PEG-TP populations.</p><h4><strong>Why CD-MS was critical</strong></h4><p>Because PEG is polydisperse, it broadens the mass distribution. In conventional ensemble MS, overlapping charge states prevent proper deconvolution. CD-MS bypasses this by assigning charge per ion, enabling:</p><ul><li>Accurate intact mass measurement</li><li>Impurity detection</li><li>Quantification</li><li>High reproducibility across runs</li></ul><p>For complex PEGylated biologics, this represents a<strong> major analytical advance</strong>.</p><h3><strong>Step 2: Mapping the pegylation sites (bottom-up strategy)</strong></h3><p>Knowing there’s one PEG is not enough — regulators and developers need to know <strong>where</strong> it’s attached.</p><p><strong>The team designed a multi-enzyme digestion workflow:</strong></p><ul><li>Lys-C digestion</li><li>Glu-C digestion</li><li>Lys-C/Glu-C combination</li><li>PNGase F treatment to remove N-glycans</li></ul><p>Each digestion generated peptides of different lengths, providing complementary information.</p><p>But there was still a problem.</p><p>PEGylated peptides are difficult to fragment because the large PEG dominates ionization and masks peptide signals.</p><h3><strong>The clever trick: high source CID</strong></h3><p>They applied <strong>high in-source collision-induced dissociation (~90 eV)</strong> to partially strip PEG chains before MS/MS.</p><p>This generated truncated PEG forms (PEG1–PEG50 variants), allowing:</p><ul><li>Precursor isolation</li><li>Efficient HCD fragmentation</li><li>Identification of diagnostic fragment ions</li></ul><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Figure 4 (page 7)</strong></a><strong> </strong>dramatically shows how high source CID transforms a messy PEG-dominated spectrum into clean multiply charged peptide precursors ready for sequencing.</p><h3><strong>The result: three pegylation sites identified</strong></h3><p>Using DDA and software-assisted analysis (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1837"><strong>Biopharma Finder</strong></a>), the study confidently identified PEGylation at:</p><ul><li><strong>K52 (major site)</strong></li><li><strong>K45</strong></li><li><strong>N-terminal A1</strong></li></ul><p><strong>K52 — The dominant site</strong><br>K52 was consistently observed across all digestion strategies. Fragment maps showed strong diagnostic ions carrying truncated PEGs (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Figures 5–7</strong></a>).<br>Multiple truncations (PEG22–PEG29 and beyond) were observed, confirming robust assignment.</p><p><strong>K45 — A secondary site</strong><br>K45 was confirmed in the combined Lys-C/Glu-C digestion (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Figure 8</strong></a>), where shorter peptides improved fragmentation and site localization.</p><p><strong>N-Terminal A1 — also modified</strong><br>Unexpectedly, PEGylation was also identified at the N-terminus (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Figure 9</strong></a>), with highly accurate fragment ion mass measurements supporting this assignment.</p><p><strong>The evidence suggests that:</strong></p><ul><li>K52 and A1 are the most frequently occupied sites</li><li>K45 is less populated</li></ul><h3><strong>Why this matters for biopharma</strong></h3><p><strong>PEGylated therapeutics must demonstrate:</strong></p><ul><li>Batch-to-batch consistency</li><li>Controlled PEGylation stoichiometry</li><li>Clear impurity profiles</li><li>Verified modification sites</li></ul><p><strong>This workflow delivers:</strong></p><ul><li><strong>Intact mass confirmation under native condition</strong></li><li><strong>Quantification of impurities</strong></li><li><strong>Confident site localization</strong></li><li><strong>High reproducibility</strong></li><li><strong>Software-assisted interpretation</strong></li></ul><p>Importantly, it requires minimal sample — a key advantage in early development.</p><h3><strong>The bigger picture: a blueprint for complex biologics</strong></h3><p><strong>The combination of:</strong></p><ul><li>Orbitrap-based Charge Detection MS</li><li>Native intact mass analysis</li><li>Multi-enzyme digestion</li><li>High source CID optimization</li><li>Advanced data processing</li></ul><p>creates a seamless, comprehensive characterization strategy.</p><p>Since pharma companies have been adding PEGylated modalities to their pipelines, the importance of this study will continue to shine. Furthermore, PEGylation and other polymer conjugations continue to evolve, and the analytical workflows must keep pace. This study demonstrates how modern mass spectrometry — particularly single-ion charge detection — can tackle extreme molecular heterogeneity with confidence.</p><p>For PEGylated therapeutics, the mass spectrometer doesn’t just weigh molecules. It tells the full molecular story.</p><blockquote><h4><strong>Reference:</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00788?goto=supporting-info"><strong>Charge Detection Mass Spectrometry and a Glu-C/Lys-C Digestion-Based Data-Dependent Approach Suggest Mono-PEGylation of a Heterogenous Therapeutic Protein</strong></a></p><p>Zoltan Szabo, Shero Lao, Katie S. Peterson, Kristina Srzentič, Kyle P. Bowen, Amanda E. Lee, Michael W. Senko, Dietmar Reusch, Steffen Lippold, and Markus Haberger</p><p><i>Journal of Proteome Research</i> <strong>Article ASAP</strong></p><p>DOI: 10.1021/acs.jproteome.5c00788</p></blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#PEGylation #Pharmacuetical #teamMassSpec #therapeutics</p>

Cracking the Code of PEGylated Proteins: How Advanced Mass Spectrometry Reveals What’s Really Attached

Pipetting Made Easy – Part 1: Best Practice Guide

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 20th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / HPLC Symposium </strong>and<strong> Waters Corporation / ASMS </strong>and technical note by<strong> KNAUER!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Comprehensive characterization of oligonucleotides and related impurities using advanced LC/Q-TOF analytical workflows</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_oligonucleotide_lc_q_tof_mp_536_asms_2026_en_agilent_b896ffee63.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Therapeutic oligonucleotides are synthetic, short nucleic acid sequences designed to modulate gene expression by targeting RNA or DNA with high sequence specificity. However, their inherent structural heterogeneity makes impurity profiling essential, as during GMP manufacturing, impurities like phosphorothioate to phosphodiester, 5′ and 3′ truncations or extensions, depurination, and abasic species can directly impact both product safety and efficacy. Antisense oligonucleotides (ASO) like Nusinersen (7127.171 g/mol) are indicated for the treatment of spinal muscular atrophy (SMA). Their closely related variants coelute with the main peak, complicating their characterization in conventional one-dimensional LC/MS workflows. To address this challenge, a 2D LC/MS strategy was implemented using the Agilent 1290 Infinity III Bio LC system with multiple heart cutting, coupled to the 6545XT AdvanceBio LC/Q-TOF and MassHunter BioConfirm software. The first-dimension separation uses an ion pairing buffer-based method to achieve higher chromatographic peak resolution, while the second dimension provides MS compatible desalting, enabling enhanced mass spectrometric sensitivity and improved detection of low-level impurities.&nbsp;</p><h4><strong>Instrumentation</strong></h4><p>1290 Infinity III Bio 2D-LC &amp; <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6545XT"><strong>6545XT Q-TOF</strong></a> includes:&nbsp;</p><ul><li>1290 Infinity III Bio Flexible Pump (G7131A)&nbsp;</li><li>1290 Infinity III Bio High-Speed Pump (G7132A)&nbsp;</li><li>1290 Infinity III Bio Multisampler (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Variable Wavelength Detector (G7114B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B)&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=2D-LC"><strong>2D-LC System</strong></a> with MHC set up</li></ul><h4><strong>Conclusions</strong></h4><ul><li>A 2D-LC/MS workflow enabled effective separation and identification of coeluting impurities.&nbsp;</li><li>HiRes sampling with multi-injection improved resolution and throughput while preserving 1D separation for MScompatible desalting.&nbsp;</li><li>6545XT AdvanceBio LC/Q-TOF delivered accurate mass and MS/MS data for low-abundance impurity detection.&nbsp;</li><li>MassHunter BioConfirm software enabled automated sequence confirmation and impurity identification.&nbsp;</li><li>This integrated platform offers a robust solution for oligonucleotide characterization in therapeutic development.</li></ul><h3><strong>2. KNAUER: Smart Monitoring &amp; Optimization Solutions for SMB Processes: In-Line and Off-Line Tools for SMB Systems</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0048_knauer_in_line_and_off_line_tools_for_smb_systems_50a5f1c1cf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In liquid chromatography, the purification of target compounds is classically carried out using single column batch chromatography. More efficient approaches include semi – continuous systems with two to three columns and continuous multicolumn systems. The most widely used of the latter is the simulated moving bed (SMB) chromatography which enables the continuous separation of binary or pseudo-binary mixtures. SMB purification is used in various areas such as petrochemical, pharmaceutical or food industry for the production of fine chemicals in different quantities and purities ranging from gram to tons per day. SMB systems vary significantly in size from small lab scale systems operating columns with 8 mm inner diameter (ID) to industrial systems operating columns of onemeter ID or larger.</p><p>Ideally, an SMB process is developed in a smaller lab scale system to reduce solvent and sample consumption compared to development at production scale. Once the separation parameters are established, the SMB process allows for accurate up scaling from lab to production scale. During method development but also production, different tools can be used to monitor and control the process which can be distinguished between in-line and off-line measures. The most crucial parameter, in addition to measuring the pressure, is monitoring the flow rates of the pumps, outlets and/or zones accurately with additional flowmeters. Normally, SMB systems are not equipped with detectors, but UV or RI detectors can be integrated to monitor process stability.&nbsp;</p><p>A standard off-line tool is used to analyse samples taken for one switch or cycle at the raffinate and extract outlets. The resulting chromatograms are used to check, if the separation is working. Further, samples can be taken during the process allowing the depiction of concentration profiles in the different zones. These tools will be described in more detail in the following sections.</p><h4><strong>MATERIAL AND METHODS</strong></h4><ul><li><strong>AZURA Lab SMB system</strong>: SMB, biocompatible, 30 ml/min zone1, 4 ml/min feed, 10 – 130 bar&nbsp;</li><li><strong>AZURA Lab SMB system: </strong>SMB, stainless steel, 30 ml/min zone1, 4 ml/min feed, 10 – 130 bar&nbsp;</li><li><strong>AZURA Pilot SMB system, standard: </strong>SMB, stainless steel, 250 ml/min zone1, 40 ml/min feed, 2 – 100 bar&nbsp;</li><li><strong>AZURA Pilot SMB system, high flow:</strong> SMB, stainless steel, 400 ml/min zone1, 100 ml/min feed, 2 – 60 bar</li><li><strong>Refractive index detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2429"><strong>AZURA® RID2.1L HighFlow detector</strong></a> 100 ml/min&nbsp;</li><li><strong>UV detector: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2428"><strong>AZURA®UVD 2.1S detector</strong></a>, w/o flow cell</li><li><strong>UV detector: </strong>AZURA®UVD2.1S UV detector, fiber optics w/o flow cell</li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>SMB is a continuous separation process that can run for several hours or days without interruption. Therefore, it is important to monitor the process and to be able to adapt to changes during the run. Different tools are available that can be used for process monitoring and method development. These tools can be divided into two main categories: in-line and off-line tools. In-line tools monitor parameters such as pressure and flow rate directly during the run, whereas off-line tools take a sample, which is then analyzed by an HPLC system. The tools described can be added to the KNAUER SMB systems depending on the customer’s needs. The more parameters that are measured, the better the process can be monitored. This is especially helpful during SMB method development.</p><h3><strong>3. Shimadzu / ASMS: Accelerating drug screening in forensic hair analysis by applying high-speed polarity switching in HR LC-MS/MS</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_180_Accelerating_drug_screening_in_forensic_hair_analysis_by_applying_high_speed_polarity_switching_in_HR_LC_MSMS_168f8cc0b2.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In 2023, the Society of Hair Testing (SoHT) published updated consensus guidelines for the forensic analysis of drugs of abuse in hair, defining recommended targets across eight major drug classes: opiates, cocaine, amphetamines, cannabinoids, opioids, methadone, buprenorphine, and ketamine. Legal drivers, accepted use cases, and degree of prescription differ between US and Europe but in both jurisdictions, mass spectrometry is widely applied to support legally defensible conclusions. The challenge for any drug testing in hair samples, whether it is in forensic and safeguarding contexts, workplace testing or a judicial setting is the need to develop methods capable of detecting expanded search panels with the increased prevalence of new psychoactive substances (NPS) and non-medical prescription drug use. The approach applied in this work considered a LC/QTOF analysis using DDAMS/MS with rapid polarity switching with a novel data processing application, Insight Profiler. The software was designed to support a single click approach to automate data processing of complex data sets in non-targeted analysis from feature detection to compound identification.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>Reversed phase LC Separation: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+X2"><strong>Nexera X2&nbsp;</strong></a></h5><ul><li>Shim-pack Velox Biphenyl (100 x 2.1 mm, 2.7 µm) column; column temp. 40 ºC, flow rate: 0.3 mL/min, 17 min total analysis time. Methanol:water gradient with 2 mM ammonium formate and 0.002% formic acid&nbsp;</li></ul><h5><strong>High resolution QTOF analysis: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-9050"><strong>LCMS-9050&nbsp;</strong></a></h5><ul><li>MS scan m/z 100-1000, 100 msec scan time. Polarity switching time 800 msec.&nbsp;</li><li>MS/MS DDA: 4 scans in positive ion mode (m/z 40-1000, 33 msec), 2 scans in negative ion mode (m/z 40-1050, 33 msec) CE 5-55V (1.998 sec total cycle time).</li></ul><h5><strong>Data processing</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Insight+Profiler"><strong>Insight Profiler</strong></a>, feature detection threshold set to low, compound identification search lists included NIST, HighResNPS, Shimadzu Forensic Toxicology database.</p><h4><strong>4. Conclusions&nbsp;</strong></h4><ul><li>The targeted DDA-MS/MS polarity switching approach successfully identified a significant number of unknown compounds in the samples not routinely targeted by triple quadrupole analysis.&nbsp;</li><li>The method using DDA-MS/MS was easily configured to support an expanded DoA target panel to meet the needs of forensic toxicology screening.&nbsp;</li><li>Non-targeted analysis using Insight Profiler accelerated data analysis by aligning identified compounds from the same hair donor showing direction of change for pharmaceutical and illicit compounds detected.</li></ul><h3><strong>4. Thermo Fisher Scientific / HPLC Symposium: Enhancing semi-volatile analyte detection in HPLC-CAD using temperature coupling mode for optimized evaporation control</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004766_hplc_vanquish_cad_fatty_acid_quantification_po004766_na_en_4a0868c2cb.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Polysorbates are widely used in biopharmaceutical formulations but can degrade or contain FAs impurities that must be accurately measured to ensure product quality and safety. Charged Aerosol Detection is commonly used for FA analysis, though its performance depends strongly on EvapT. Higher EvapT improves S/N ratios but can reduce sensitivity for semi-volatile compounds like FAs, while lower EvapT has the opposite effect. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2176"><strong>Thermo Scientific Vanquish Charged Aerosol Detector P</strong></a> series introduces a Temperature Coupling Mode that links EvapT with the charging detection module (CDM) temperature, overcoming limitations of earlier systems with fixed CDM temperature of 40 ºC. This coupling improves response and measurement reliability for semi-volatile species, enabling better optimization of FA analysis<sup>1</sup>.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>Advanced temperature control in CAD improved method sensitivity up to ~47% for semi-volatile fatty acids.&nbsp;</li><li>The best overall S/N was achieved at 25.0 °C EvapT with Temperature Coupling Mode enabled.&nbsp;</li><li>The optimized CAD approach supports more sensitive FA impurities and degradants quantification in pharmaceutical and biopharmaceutical formulations.</li></ul><h3><strong>5. Waters Corporation / ASMS: High mass and spatial resolution MS imaging of soft fruit sample cross-sections using desorption electrospray ionization (DESI)</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009492_282619e388.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>This poster demonstrates the use of <strong>desorption electrospray ionization mass spectrometry imaging (DESI MSI)</strong> for visualizing the spatial distribution of metabolites in high-water-content soft fruits, specifically strawberries and raspberries. The authors developed a simple direct-transfer workflow to overcome the challenges associated with imaging delicate fruit tissues and compared three transfer substrates: plain glass microscope slides, nitrocellulose membranes, and Hamamatsu Poropare plates. The goal was to determine which surface best preserves metabolite localization while enabling high-quality mass spectrometry imaging.</p><p>Fresh fruit cross-sections were transferred to each substrate by direct contact for 10 seconds before analysis using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=DESI+XS"><strong>Waters DESI XS ion source</strong></a> coupled to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2433"><strong>Waters Xevo™ MRT P10 high-resolution mass spectrometer</strong> </a>operating in negative electrospray ionization mode. MS imaging was performed with a <strong>50 µm pixel size</strong>, using a <strong>95:5 methanol/water</strong> spray solvent, while sucrose (m/z 341.1089) served as the lock mass for accurate mass calibration. High mass resolution enabled tentative assignment of metabolites such as citric acid, asparagine, malic acid, glutamic acid, palmitic acid, stearic acid, and sucrose.</p><p>The comparison of transfer substrates revealed significant differences in metabolite localization and image quality. Glass slides produced the strongest metabolite signals but also exhibited considerable metabolite delocalization during sample transfer, reducing spatial accuracy. Nitrocellulose membranes transferred a broader range of metabolites and provided complementary chemical information, although they also showed greater analyte spreading and suffered physical damage after a single DESI acquisition. In contrast, <strong>Poropare plates</strong> minimized metabolite delocalization and preserved fine structural details within the fruit cross-sections, despite producing somewhat lower signal intensities than glass slides.</p><p>The study concludes that direct transfer combined with DESI MSI is an effective approach for imaging soft fruit tissues that are otherwise difficult to analyze because of their high water content. Among the evaluated substrates, <strong>Hamamatsu Poropare plates</strong> provided the best balance between spatial resolution and preservation of metabolite localization, making them particularly suitable for high-resolution metabolomic imaging, while high mass accuracy facilitated confident tentative metabolite identification.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 30, 2026

Article

Product

Academy

Science and research

Poster

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Phenomenex

SelectScience

C&EN

Shimadzu Corporation

SCIEX

Waters Corporation

<h4><strong>Three professors. One topic. New perspectives.</strong></h4><p>We're bringing the classroom straight to you with <strong>From the lecture hall: Mass spectrometry fundamentals.</strong></p><p>This 3-part webinar series brings you inside a virtual classroom led by professors from top-tier institutions, who will break down the basics of mass spectrometry. Zero dull moments and no heavy textbooks required.</p><p>Whether you’re new to mass spectrometry or looking to brush up on your knowledge, you’ll walk away with new perspectives.</p><h5><strong>Join us to learn about:</strong></h5><ul><li>Basic concepts from mass-to-charge ratio to resolution</li><li>Key instrumentation such as triple quadrupoles and time-of-flight systems</li><li>Real-world applications</li><li>Interpreting data with confidence</li></ul><p>It’s not every day you get a seat in a classroom like this.</p><blockquote><p><strong>Speaker:&nbsp;</strong>Alec Saitman, PhD (Oregon Health and Science University)</p></blockquote>

SCIEX: From the lecture hall: Mass spectrometry fundamentals

From the lecture hall: Mass spectrometry fundamentals - Part II

Learn mass spectrometry fundamentals in a 3-part webinar series covering instrumentation, data interpretation, and practical applications.

From the lecture hall: Mass spectrometry fundamentals II

<h4><strong>Three professors. One topic. New perspectives.</strong></h4><p>We're bringing the classroom straight to you with <strong>From the lecture hall: Mass spectrometry fundamentals.</strong></p><p>This 3-part webinar series brings you inside a virtual classroom led by professors from top-tier institutions, who will break down the basics of mass spectrometry. Zero dull moments and no heavy textbooks required.</p><p>Whether you’re new to mass spectrometry or looking to brush up on your knowledge, you’ll walk away with new perspectives.</p><h5><strong>Join us to learn about:</strong></h5><ul><li>Basic concepts from mass-to-charge ratio to resolution</li><li>Key instrumentation such as triple quadrupoles and time-of-flight systems</li><li>Real-world applications</li><li>Interpreting data with confidence</li></ul><p>It’s not every day you get a seat in a classroom like this.</p><blockquote><p><strong>Speaker:&nbsp;</strong>Adina Badea, PhD, DABCC (Brown University)</p></blockquote>

From the lecture hall: Mass spectrometry fundamentals - Part III

From the lecture hall: Mass spectrometry fundamentals III

<div><h4><strong>Latest advances in RT-MALS</strong></h4><p><strong>The ultraDAWN RT-MALS sensor integrates inline or online with downstream unit operations to monitor essential biophysical attributes such as molecular weight, nanoparticle size, physical titer and – for AAV or similar small viral vectors/VLPs – full capsid ratio, empty and full titer and more. Now, with the addition of an embedded dynamic light scattering (DLS) module, an ultraDAWN reveals even more product attributes in real time: hydrodynamic size and polydispersity.</strong></p><p>In addition to RT-DLS, we introduce the latest RT-MALS product – ultraDAWN Pilot - designed for inline operation with pilot scale DSP unit ops such as preparative chromatography or LNP-mRNA encapsulation.</p><h4><strong>What you will learn:</strong></h4><ul><li>How RT-MALS/RT-DLS accelerates process development, deepens process knowledge and enables model-free, CQA-based process control for recombinant proteins, viral vectors, LNPs and polysaccharide conjugate vaccines</li><li>How the ultraDAWN integrates with DSP unit ops inline at bench scale, and online at any scale.</li><li>How the ultraDAWN’s high measurement frequency means more complete process characterization and faster feedback for process control.</li><li>How the ultraDAWN Pilot enables smooth transfer of light-scattering-based PAT methods from PD to production.</li></ul><h4><strong>Who should attend?</strong></h4><p>This webinar is designed for :</p><ul><li>Scientists, Engineers, PAT leads and MSAT personnel involved in downstream process development or production of recombinant proteins, viral vectors, VLPs, LNPs, biologics, polysaccharides, etc.</li><li>CDMO, CRO and other organization who are interested in streamlining process development and production.</li></ul><blockquote><p><strong>Presenter: </strong>Daniel Some, Ph. D.<strong> </strong>(Sr. Principal Product Manager, Waters | Wyatt Technology)</p><p>Dan Some currently leads product, application and market development for Waters’ real-time light scattering product line.</p><p>Previously he contributed to Wyatt’s scientific and technical marketing, and developed instrumentation and software for characterizing biomolecular interactions.</p><p>Dan obtained his Ph.D. in physics from Brown University, then carried out postdoctoral research at Los Alamos National Laboratory and the Weizmann Institute of Science. Prior to joining Wyatt he was involved in defense technology and the semiconductor industry.</p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Udayabagya (Bud) Halim (Sr. Product Marketing Analyst, Waters Corporation)</p><p>Bud Halimis a senior marketing analyst at Waters | Wyatt Technology with a PhD in physical chemistry from UCLA.</p><p>He began his career teaching biochemistry and chemistry and contributing to laboratory manuals before transitioning into the scientific instrumentation industry as an applications scientist.</p><p>At Waters, he supports researchers in the characterization of peptides, biologics, lipid nanoparticles (LNPs), and viral vectors.</p></blockquote></div>

Waters Corporation: Scale up, see more - latest advances in real-time light scattering as inline/online PAT for downstream bioprocess

Scale up, see more: latest advances in real-time light scattering as inline/online PAT for downstream bioprocess

Learn how ultraDAWN RT-MALS and RT-DLS accelerate bioprocess development. Monitor CQAs like full/empty capsid ratios and LNP size in real time

Real-Time Light Scattering (RT-MALS) as Bioprocess PAT

<p><strong>High‑throughput LC‑MS/MS workflows using complex matrices are often limited by column degradation, leading to poor peak shape, rising backpressure, and unplanned downtime that impact productivity and cost. &nbsp;Endrix LC columns address these challenges through a hardware‑driven approach, combining proven Kinetex and Luna Omega chemistries with proprietary design to extend usable column lifetime without compromising performance. &nbsp;Real‑world results from a 75‑analyte pain management panel demonstrate significantly increased column lifetime while maintaining consistent chromatographic integrity. &nbsp;By reducing column replacement frequency and minimizing troubleshooting, Endrix enables greater instrument uptime, improved efficiency, and lower cost per injection in demanding, high‑throughput workflows.</strong></p><h4><strong>Key Learning Points</strong></h4><ul><li>Identify key factors that limit LC column lifetime in high‑throughput and complex‑matrix workflows</li><li>Understand how hardware design impacts column durability, beyond stationary‑phase chemistry</li><li>Implement strategies to extend column lifetime, reduce downtime, and lower cost per injection</li><li>Assess when guard columns are optional and how to simplify workflows without sacrificing performance</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any LC-MS/MS scientists in clinical, toxicology, and pharmaceutical laboratories, as well as analytical chemists working with complex or “dirty” matrices such as urine, plasma, and tissue samples.</li></ul><h4><strong>Viewing Options</strong></h4><h5><strong>Session 1</strong></h5><p><strong>Tuesday, July 21, 2026</strong></p><ul><li>Los Angeles: 10:00 AM PT | New York: 1:00 PM EST<br>Mexico City: 12:00 PM CDT</li></ul><h5><strong>Session 2</strong></h5><p><strong>Wednesday, July 22, 2026</strong></p><ul><li>London: 9:00 AM BST | Berlin: 10:00 AM CEST&nbsp;<br>Mumbai: 1:30 PM IST</li></ul><blockquote><p><strong>Presenter: </strong>Grace Guo &nbsp;(Senior Technical Specialist, Phenomenex)</p><p>&nbsp;Grace Guo graduated from Johns Hopkins University with a Master’s degree in Material Science and Engineering. She joined Phenomenex Technical Team in 2017 providing technical support globally. With more than 9 years of experience in SPE, HPLC and GC, she delivered 90+ seminars and webinars globally covering food and environmental, clinical, pharma and biopharma industry.&nbsp;</p></blockquote>

Phenomenex: From Downtime to Data: How Long‑Life Endrix Columns Transform High‑Throughput Labs

From Downtime to Data: How Long‑Life Endrix Columns Transform High‑Throughput Labs

Discover how Phenomenex Endrix LC columns transform high-throughput workflows. Extend column lifetime and maintain peak shape in complex-matrix LC-MS

Long-Life Endrix LC Columns for High-Throughput Labs

<h4><strong>Scaling LC Methods Sustainably</strong></h4><p><strong>Reducing solvent consumption is a growing priority for modern laboratories but should not come at the expense of performance. In this webinar, you will learn how to maintain recovery, sensitivity and reproducibility with MaxPeak Premier columns in new 300 µm, 1.0 mm, and 3.0 mm formats for LC workflows that are more efficient and sustainable.</strong></p><p>Discover how the dimensions of these columns support scalable method development, reduce waste and operating costs, and improve data confidence, especially for challenging, metal-sensitive analytes.</p><h4><strong>What you will learn:</strong></h4><ul><li>How to reduce solvent consumption and waste across LC and LC-MS workflows without sacrificing performance</li><li>Applications where 300 µm, 1.0 mm, and 3.0 mm columns can drive improved efficiency and sustainability.</li><li>How MaxPeak Premier Technology improves recovery and repeatability, especially for challenging, metal-sensitive analytes</li></ul><h4><strong>Who should attend</strong></h4><ul><li>Analytical scientists/method developers looking to reduce solvent use without losing performance&nbsp;</li><li>LC and LC-MS practitioners working with metal-sensitive analytes who desire improved recovery and/or reproducibility&nbsp;</li><li>Lab directors/managers focused on workflow efficiency, &nbsp;operational costs and sustainability</li><li>Scientists focused on developing, transferring, and scaling methods across varying column dimensions and/or LC platforms</li></ul><blockquote><p><strong>Speaker: </strong>Chris Collins, Ph.D. (Principal Product Marketing Manager, Waters Corporation)</p><p>Chris Collins, Ph.D. earned his Ph.D. in Analytical Chemistry from North Carolina State University in 2014. He has over 20 years experience in the pharmaceutical industry with a focus on LC/MS Method Development.</p><p>Chris started working at Waters Corporationin2018 as a Chemistry Account Manager for North Carolina/South Carolina. In 2023, he transitioned to the role of Principal Product Marketing Manager for ACQUITY UPLC columns and MaxPeak Premier Column Technology.</p></blockquote><blockquote><p><strong>Speaker:&nbsp;</strong>Keil Brinster (Senior Product Marketing Manager, Waters Corporation)</p><p>Moderated by Keil B., Senior Product Marketing Manager at Waters.</p></blockquote>

Next Level LC Efficiency: Reduce Solvent, Maximize Recovery

Discover sustainable LC method scaling with Waters MaxPeak Premier columns. Reduce solvent consumption and improve recovery for metal-sensitive analytes

Next Level LC Efficiency: Reduce Solvent & Maximize Recovery

<p><strong>The multi-attribute method (MAM) is a powerful approach to monitoring critical quality attributes (CQAs) and detecting product variants in a single, streamlined biopharmaceutical workflow. However, widespread adoption in QC has been limited by method complexity, high costs, and operational burden.</strong></p><p>Join Prof. Jared Auclair of Northeastern University and Melissa Sato of Agilent Technologies in this SelectScience® webinar as they present a practical path forward – the Agilent MAM.</p><p>The Agilent MAM solution addresses these challenges by combining purpose-built Agilent MAM for OpenLab CDS software with robust, highly efficient Agilent LC/MS systems, enabling the confident transition of MAM from R&amp;D into regulated QC environments. Designed according to USP &lt;1060&gt;, this comprehensive solution includes software, LC/MS instrumentation, consumables, and built-in system performance checks to simplify QC method implementation, reduce training requirements, and improve QC turnaround time.</p><h5><strong>Certificate of attendance</strong></h5><p>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</p><h5><strong>Webinar details</strong></h5><ul><li>Cost: Free to attend</li><li>Location: Online</li><li>Duration: 60 minutes</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>Lab managers</li><li>Quality control professionals</li><li>Biopharmaceutical R&amp;D professionals</li></ul><h5><strong>What will this webinar cover?</strong></h5><ul><li>Pharmaceutical regulatory guidelines outlined by USP &lt;1060&gt;</li><li>The key operational challenges of implementing MAM in QC and how a purpose-built solution mitigates complexity, training needs, and compliance risk</li><li>How this solution includes built-in system performance checks and new peak detection to ensure reliable, routine MAM execution</li><li>How to evaluate method transfer from R&amp;D to QC to reduce rework, improve consistency, and accelerate batch release</li><li>How a novel new peak detection algorithm minimizes false positives while still providing sufficient limits of quantitation</li></ul><p><i>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.</i></p><blockquote><p><strong>Speaker:</strong> Prof. Jared Auclair (Dean, Northeastern University College of Professional Studies, Director, Bioinnovation)</p><p>Prof. Jared Auclair holds a PhD in Biomedical Science from the University of Massachusetts Medical. He now serves as the Dean of the College of Professional Studies, a professor at Northeastern University and, most recently, Vice Provost Research Economic Development and Director of Bioinnovation.</p></blockquote><blockquote><p><strong>Speaker: </strong>Melissa Sato (BioPharma Software Workflow Manager, Agilent Technologies)</p><p>Melissa Sato brings extensive biopharma experience specializing in analytical sciences, including mass spectrometry and chromatography for mAbs, BiTEs, fusion proteins, and oligonucleotides. She also brings over 10 years of experience in multi-attribute method expanding across development to GMP from her previous roles at Amgen and Thermo Fisher Scientific. &nbsp;Now at Agilent, she leads the MAM for OpenLab CDS product and oversees BioConfirm and ExDViewer, driving innovation in biopharma mass spectrometry applications.</p></blockquote><blockquote><p><strong>Moderator:</strong> Olivia Long</p><p>Olivia Long is a science editor specializing in pharmaceutical and applied science. She has a BSc in Pharmacology, and an MSc in Neuroscience, where she investigated neuro‑oncology drug‑resistance pathways using patient‑derived cell models. Her laboratory experience spans R&amp;D hygiene testing, controlled‑environment sample production, and protocol development, giving her a broad understanding of analytical workflows and the scientific principles underpinning biopharmaceutical quality control.</p></blockquote>

Agilent Technologies: Implement multi-attribute methods with confidence – A solution designed for biopharma QC labs

Implement multi-attribute methods with confidence – A solution designed for biopharma QC labs

Transition MAM from R&D to regulated QC. Discover Agilent's comprehensive LC/MS and OpenLab CDS solutions for streamlined biopharma CQA monitoring

Implementing Multi-Attribute Methods in Biopharma QC

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>When (bio)pharma formulation components have little or no UV response, HPLC methods can take longer to develop and optimize, become harder to transfer, and leave uncertainty around critical results. The right universal LC detector can help reduce method development effort, improve detection confidence, and support more robust routine workflows.</strong></p><p>Using one of the most challenging formulation excipients to characterize as a concrete example, we will walk you through how to analyze Polysorbate 80 using <strong>Thermo Scientific™ Vanquish™ Charged Aerosol Detector (CAD) </strong>and compare with results generated by ELSD. Additional case studies from lipid nanoparticle sample testing will show how the two detectors compare in practice, explain key differences in parameter settings, and offer practical advice on how to evolve your methods from ELSD to CAD.</p><p>Join us for a practical guide to evaluate a more robust path for detecting challenging analytes in your formulation.</p><h4><strong>Who should attend:</strong></h4><ul><li><strong>HPLC users who rely on ELSD</strong> but struggle with time-consuming optimization, sensitivity limits, noisy baselines, reproducibility, or transfer of methods into QC</li><li><strong>Analytical development, QC, and formulation teams </strong>working with complex (bio)pharma excipients, polysorbates, lipid nanoparticle formulations, related lipids, impurities, degradants, or other analytes with little or no UV response.</li></ul><h4><strong>Attendees will gain practical strategies to:</strong></h4><ul><li><strong>Improve detection confidence</strong> for low-abundance, non-chromophoric analytes</li><li><strong>Build more robust, transferable HPLC methods</strong> for development and QC workflows</li><li><strong>Reduce method transfer risk </strong>by understanding which detector settings affect ELSD-to-CAD performance</li></ul><p><strong>Register now to gain practical guidance for improving confidence in universal HPLC detection.</strong></p><blockquote><p><strong>Presenter: </strong>Chris John, PhD (Research Scientist, Thermo Fisher Scientific)</p><p>Chris John received his Ph.D. in Chemistry from Michigan State University in 2019. He then joined PPD’s Biopharmaceuticals Method Development team where he led the development and validation of HPLC methods for quantifying excipients, impurities, and APIs in biopharmaceutical products. Chris later transferred to PPD’s Cell and Gene Therapy group as an HPLC subject matter expert where he continues to lead method development and validation activities.</p></blockquote><blockquote><p><strong>Presenter: </strong>Adam Punke (Sr. Research Scientist, Thermo Fisher Scientific)</p><p>Adam Punke has 15+ years of experience conducting HPLC/UPLC analyses in a GMP Environment. After obtaining his bachelor’s degree in chemistry and biology at the University of Wisconsin, he joined PPD’s inhalation department and performed testing to support stability studies of dry powder inhaled (DPI) products.</p><p>In 2011, Adam transitioned to PPD’s Biopharmaceuticals group to lead stability testing associated with a protein-based DPI. In 2015 Adam earned his master’s degree in chemistry from the University of North Carolina.</p><p>He is currently an HPLC subject matter expert in the method development and validation team in the PPD Gene and Cell Therapy department where he oversees method development/validation projects and provides internal support to troubleshoot chromatographic issues.</p></blockquote></div></div></div>

Thermo Fisher Scientific: How to save time and gain confidence in complex excipient analysis with universal LC detection

How to save time and gain confidence in complex excipient analysis with universal LC detection

Discover how Vanquish CAD improves HPLC detection of non-UV analytes and compare its performance with ELSD for biopharmaceutical analysis.

Save time and gain confidence with universal LC detection

<p><strong>Are your titration results keeping up with the demands of modern petrochemical operations? Many facilities still rely on manual or lab-only titration workflows, limiting data traceability, delaying response to process upsets, and increasing exposure to corrosion, compliance risk, and avoidable rework. Automated titration changes that, but the real opportunity is realized when validated lab methods are extended into the process.</strong></p><p>Join Brett Lane and Eduardo Simões from Metrohm USA and Kris Kohl, Lead Technical Support Engineer at Baker Hughes, for office hours focused on lab-to-process titration in the petrochemical industry. Drawing on real-world field experience, these experts will walk through three high‑impact applications: crude overhead water analysis (ammonia, chloride, and iron), mercaptan measurement, and total acid and base number (TAN/TBN). You will see how lab methods translate into a continuous process analyzer and what that means for your accuracy, repeatability, data traceability, and ROI across lab, process, and plant operations.</p><p>Bring your questions to the live Q&amp;A focused on real lab‑to‑process titration decisions.</p><h4><strong>Key Learning Objectives:</strong></h4><ul><li>Understand the distinct and complementary roles of lab and process titration</li><li>See how key titration applications translate from lab to process in petrochemical operations</li><li>Learn how automaton improves accuracy, repeatability, and data traceability</li><li>Explore the ROI of process titration through real-world field experience from Baker Hughes</li><li>Identify which applications in your own operation could benefit from extending lab methods into the process</li></ul><h4><strong>Who Should Attend:</strong></h4><ul><li>Laboratory analysts and chemists performing titration-based quality control and routine sample analysis</li><li>Process engineers evaluating or managing inline and at-line measurement for real-time process control</li><li>Plant managers and technical decision makers assessing the ROI of process analytical technology</li><li>Environmental, health, and safety professionals responsible for sampling protocols and safe operation in hazardous areas</li><li>Anyone in the petrochemical industry working with crude overhead water, mercaptans, or TAN/TBN applications</li></ul><blockquote><p><strong>Presenter: </strong>Brett Lane (Field Applications Specialist, Metrohm USA)</p><p><strong>Presenter:&nbsp; </strong>Eduardo Simões, MSc (Titration Product Specialist, Metrohm USA)</p><p><strong>Presenter:&nbsp;</strong>Kris Kohl (Lead Technical Support Engineer, Baker Hughes)</p><p><strong>Presenter:&nbsp;</strong>Melissa O'Meara (Forensic Science Consultant, ACS Media Group)</p></blockquote>

Metrohm: Office Hours: Lab and Process Titration for the Petrochemical Industry

Office Hours: Lab and Process Titration for the Petrochemical Industry

Explore lab-to-process titration for petrochemical analysis, including crude overhead water, mercaptans, TAN/TBN, automation and ROI.

Lab and Process Titration for Petrochemical Industry

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>UV detection has traditionally been the standard approach for protein analysis in capillary electrophoresis (CE) workflows used in biopharmaceutical development and quality control. However, fluorescence-based detection methods are increasingly being adopted to address analytical challenges that require greater sensitivity and selectivity.</strong></p><p>In this live webinar, you will learn how the two main fluorescence detection approaches used in CE—native fluorescence (NF) and laser-induced fluorescence (LIF)—work and where they can provide advantages over conventional UV detection. Through practical application examples, we will explore how fluorescence detection can support protein characterization workflows, including charge heterogeneity and fragment analysis.</p><h4><strong>What you will learn</strong></h4><ul><li>The principles of native fluorescence (NF) and laser-induced fluorescence (LIF) detection in CE</li><li>Key differences between fluorescence and UV detection methods</li><li>When fluorescence detection can provide greater sensitivity and selectivity</li><li>Applications of NF and LIF for protein analysis in biopharmaceutical workflows</li><li>Real-world examples of charge heterogeneity and fragment analysis using fluorescence detection</li><li>Considerations for selecting the most appropriate detection strategy for your analytical goals</li></ul><blockquote><p><strong>Presenter: </strong>Stephen Lock (Sr. Market Development Manager – Biopharma, SCIEX)</p><p>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry.</p><p>He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world.</p><p>He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p></blockquote></div></div></div>

SCIEX: Understanding the benefits of fluorescence detection in capillary electrophoresis (CE)

Understanding the benefits of fluorescence detection in capillary electrophoresis (CE)

Explore NF and LIF detection in capillary electrophoresis for biopharma protein analysis, charge heterogeneity and fragment analysis.

Fluorescence detection in capillary electrophoresis

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Accelerating the adoption of renewable fuels is central to the global energy transition, but analyzing complex matrices like Sustainable Aviation Fuel (SAF) and biodiesel can pose new challenges.</strong></p><p>In this latest session on Shimadzu’s Digital Classrooms, discover practical strategies into navigating these shifts with Shimadzu's latest innovations. Get insights into how to streamline fuel characterization, ensure strict quality control, and maintain asset reliability using the new Nexis GC-2060, Transformer Oil Gas Analysis (TOGAS) systems, dedicated System GC solutions, GC-SCD sulfur analysis, and LC-based technologies.</p><p>We will also demonstrate how to expand your laboratory's R&amp;D capabilities using Plasmion SICRIT® technology for direct GC-LCMS coupling—giving you the advanced insights needed to optimize both conventional petrochemicals and next-generation renewable fuels. Register Now!</p><h4><strong>Learning Objectives</strong></h4><ul><li>Explore applications of Transformer Oil Gas Analysis (TOGAS) for transformer condition monitoring and energy infrastructure reliability</li><li>Discover Shimadzu's latest Nexis GC-2060 and System GC solutions for energy, refinery, petrochemical, and fuel testing laboratories</li><li>Applications of GC, LC, and GC-SCD technologies in HPI, SAF, and biodiesel analysis</li><li>Understand how SICRIT® technology enables direct GC coupling to LC-MS systems for advanced fuel and petrochemical research</li></ul><blockquote><p><strong>Presenter: </strong>Peter, Puah Perng Yang (Market Specialist Chemical, Shimadzu Asia Pacific)</p><p><strong>Presenter:&nbsp;</strong>Elvi Horiyanto (Product Specialist, GC, Shimadzu Asia Pacific)</p><p><strong>Presenter:&nbsp;</strong>Dr. Jan Bucek (Head of Global Sales, Plasmion GmbH)</p></blockquote></div></div></div>

Shimadzu: Powering Energy Security and the Energy Transition: Advanced Analytical Solutions for Petroleum and Renewable Fuels

Powering Energy Security and the Energy Transition: Advanced Analytical Solutions for Petroleum and Renewable Fuels

Shimadzu webinar on SAF, biodiesel and petrochemical analysis using GC, LC, GC-SCD, TOGAS and SICRIT GC-LCMS coupling.

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