<div><p><strong>Air bubbles in an HPLC system can disrupt pumping reliability, cause baseline noise, and result in poor result reproducibility and valve function. To prevent these, proper mobile phase degassing is essential.&nbsp;</strong></p><p>Degassing in HPLC not only ensures stable pumping but also improves sensitivity and accuracy by preventing dissolved oxygen from forming bubbles that interfere with detectors such as UV-VIS, fluorescence, refractive index, or electrochemical. Effective HPLC solvent degassing is therefore a critical step for stable, accurate, and reproducible chromatography.</p></div><div><div><div><div><h3><strong>What is HPLC Solvent Degassing and Why is it Necessary?</strong></h3></div></div></div></div><div><p>When solvents such as water, buffers, acetonitrile, and methanol contact the atmosphere, nitrogen and oxygen dissolve into them. When mixed, their combined air content can exceed the solubility of the mixture, creating a supersaturated condition that leads to outgassing and bubble formation. These bubbles disrupt <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc">HPLC</a> performance by causing unstable baselines, bad peak shape, pump cavitation, retention time shifting, and loss of sensitivity.</p><p>In pumps, air can stop flowing or cause erratic retention times, while in optical detectors, bubbles scatter light and generate noise or false signals. Although it is not necessary to remove all dissolved air, degassing in HPLC lowers gas levels below supersaturation and prevents these issues. Low-pressure mixing systems are especially prone to bubble formation, whereas high-pressure mixing reduces outgassing because solvents blend under elevated pressure.</p></div><div><div><div><div><h3><strong>Types of Degassing Techniques in HPLC</strong></h3></div></div></div></div><div><p>Degassing the mobile phase was once a major concern in liquid chromatography with outgassing problems being among the most common issues during the first decades of its use. The introduction of inline degassers in HPLC in the past 15–20 years has significantly reduced the occurrence of these problems, to the point that many users today have little experience with them. However, while inline degassing prevents most solvent outgassing issues, it does not eliminate all problems caused by dissolved air.</p></div><div><div><div><div><h4><strong>Inline Degassing</strong></h4></div></div></div></div><div><p>Inline degassing works by passing the mobile phase through a gas-permeable membrane within a vacuum chamber, allowing dissolved gases to escape while retaining the liquid. Different techniques include:</p><ul><li><strong>Vacuum degasser:</strong> Inline vacuum degassers, now standard in most modern HPLC systems, have greatly reduced bubble-related pump issues by efficiently removing dissolved gases. Solvents pass through porous tubing within a vacuum chamber, where gases diffuse out through micropores to waste, while the degassed liquid continues flowing onto the pump.</li><li><strong>Membrane degasser:</strong> Over the years, various polymer materials have been tried with varying success for degassers. Modern degassers use a thin, highly gas-permeable membrane, making the degassing process very efficient.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>Inline degassers are highly reliable and effective at reducing dissolved gases in the mobile phase to acceptable levels and are now standard in most HPLC systems. It is usually automatic and trouble-free. They require little to no maintenance, making them convenient and reliable for routine use.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>Despite their effectiveness, inline degassers do not remove all dissolved air from the mobile phase. The residual air can still cause problems in certain sensitive applications. Membrane replacement, vacuum pump failure, or damaged tubing can also cause issues, so proper maintenance is essential to avoid costly repairs. Additionally, microbial growth in the buffer can block membrane pores and contaminate the entire HPLC system. To prevent microbial growth, contamination, or blockages, it is best not to store the degasser with water or buffer in the tubing and to flush with 30% phosphoric acid followed by water if contamination occurs.</p></div><div><div><div><div><h4><strong>Offline Degassing</strong></h4></div></div></div></div><div><p>Offline degassing involves removing dissolved air from the mobile phase before it enters the HPLC system. The techniques include:</p><ul><li><strong>Helium sparging:</strong> This method requires a solvent inlet filter to prevent particulates and improve gas dispersion, but the degassed solvent can reabsorb air once in the system. To reduce bubble formation, slight back pressure should be maintained, often using a backpressure regulator after the detector. Helium purging can remove <strong>up to 80% of dissolved air</strong>, requiring equal helium volume for organic–aqueous phases. However, the flow rate should be reduced over time to avoid losing volatile components.</li><li><strong>Vacuum filtration:</strong> Vacuum degassing removes <strong>over 60%</strong> of dissolved air from the mobile phase and is performed through batch-wise treatment. It can also be done during filtration, before the chromatographic process with a 0.45 or 0.22 μm membrane filter, though this method is less effective.</li><li><strong>Sonication:</strong> Ultrasonic baths alone are not effective for degassing, as they remove<strong> only about 20-30% of dissolved gases</strong>, while at least 50% removal is needed. However, when combined with helium sparging or vacuum degassing, sonication becomes a <strong>useful complementary method</strong>. Since ultrasonic baths are common in laboratories, they are best applied alongside other degassing techniques.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>An offline degasser offers the advantage of using a 2 µm solvent inlet filter to prevent particulates from entering the mobile phase while ensuring efficient gas dispersion. It is also cost-effective and flexible, as it relies on standard laboratory equipment and can handle large solvent volumes in a batch.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>A major drawback of offline helium sparging is that the mobile phase can gradually reabsorb air once placed in the HPLC system, leading to signal loss and changing sensitivity. Additionally, helium sparging can be expensive as it requires specialized gas cylinders.</p><h3><strong>Inline vs Offline Degassing: Side-by-Side Comparison</strong></h3><h5><strong>Timing:</strong></h5><ul><li><strong>Offline Degassing: </strong>It is usually done before the mobile phase enters the HPLC run.</li><li><strong>Inline Degassing: </strong>It is a continuous process that occurs during an HPLC run.</li></ul><h5><strong>Degassing Efficiency:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The efficiency can be variable. For instance, vacuum/sonication removes 60–70% of dissolved air, sonication alone removes <strong>20–30%</strong> of air and helium sparging removes 80% of air.</li><li><strong>Inline Degassing:&nbsp;</strong>It does not remove 100% of dissolved gas, but it removes most of it. However, it prevents bubble formation.</li></ul><h5><strong>Risk of Re-dissolving:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The risk is high as solvents can reabsorb air when exposed to the atmosphere.</li><li><strong>Inline Degassing:&nbsp;</strong>The risk is low as the continuous vacuum keeps new gas from dissolving.</li></ul><h5><strong>Cost:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Low initial investment and requires only basic lab equipment. <strong>Helium</strong> sparging can be expensive as it requires specialized gas cylinders.</li><li><strong>Inline Degassing:&nbsp;</strong>Higher initial cost (dedicated degasser unit and vacuum pump).</li></ul><h5><strong>Ease of Use:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Manual setup each time which is time-consuming.</li><li><strong>Inline Degassing:&nbsp;</strong>Automated after installation.</li></ul><h5><strong>Impact on Solvent Composition:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Some loss of volatiles is possible such as evaporation during sparging.</li><li><strong>Inline Degassing:&nbsp;</strong>Solvent composition remains unchanged.</li></ul><h5><strong>Effect on Chromatographic Stability:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Improves initial stability but may allow bubble re-formation later.</li><li><strong>Inline Degassing:&nbsp;</strong>Very stable baselines and retention. Bubble-related problems are virtually eliminated.</li></ul><div><div><div><div><h3><strong>When to Choose Offline vs Inline Degassing</strong></h3></div></div></div></div><div><p>The choice between an offline and inline HPLC degasser depends on the situation, including factors like routine use, troubleshooting needs, and method sensitivity.</p><ol><li>Offline degassing is suitable for occasional use, limited equipment setups, or when routine inline degassing is not available. While inline degassing is preferred for routine analyses requiring consistent performance and high sensitivity.</li><li>Inline degassers have a higher initial cost but save labor. An offline degasser uses low-cost labware and is cheap to set up, though helium or other gases add to the consumable cost.</li><li>If mobile phases contain volatile organics or labile salts, avoid aggressive offline sparging/vacuum (which can evaporate components). Inline vacuum degassing is gentler on the solvent composition.</li><li>Oxygen in the mobile phase is particularly problematic for sensitive methods, such as low-wavelength UV or reductive electrochemical detection. While techniques like helium sparging and inline degassing effectively remove oxygen, remember that offline-sparged solvents are susceptible to reabsorbing air over time, which can lead to signal loss and subtle changes in sensitivity.</li></ol></div><div><div><div><div><h3><strong>Troubleshooting Degassing Issues in HPLC</strong></h3></div></div></div></div><div><h4><strong>1) Cycling of output (pressure or baseline)</strong></h4><p>Cycling patterns in pressure or the chromatogram baseline often indicate poor compressibility at the pump heads. This may result from insufficient degassing efficiency, where adding a second degassing chamber can help. It can also cause air to enter the system due to improperly connected tubing to or from the HPLC solvent degasser. Also, improper connections of the tubing on the HPLC solvent degasser can also allow air to enter the system, causing fluctuations in pressure or baseline.</p><h4><strong>2) Baseline noise</strong></h4><p>Noise on the baseline of a chromatogram may indicate problems with the gas flow system. High gas flow rates can cause short-term noise, while baseline drift (long-term fluctuations) may be due to contaminants being flushed out from a partially clogged gas line. Additionally, an unstable gas flow output can also contribute to baseline noise.</p><h4><strong>3) Leakages</strong></h4><p>Leaks often occur due to incompatibility between solvents and degasser materials. For instance, tetrahydrofuran (THF) can dry and crack degasser membranes. Additionally, improperly seated tubing connections in or out of the degasser can allow leaks to develop.</p><h4><strong>4) Blockages</strong></h4><p>Blockages create pressure ripples as the pump struggles to draw solvent, sometimes causing cavitation. They may result from algae growth, crystallization of buffer salts, or clogged inlet filters. Troubleshooting involves disconnecting outlet tubing to check for continuous solvent flow, while prevention requires filtering solvents, using clean solutions, and flushing lines regularly, especially after system shutdowns.</p><h4><strong>5) General considerations</strong></h4><p>Degasser issues are often linked with the mobile phase quality. Using poorly prepared or contaminated solvents can lead to persistent problems. Maintaining clean, filtered, and fresh solvents helps ensure smooth operation and minimizes downtime.</p></div></div>

Degassing Techniques in HPLC: Inline vs Offline

Everything to know about osmolality

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / HPLC Symposium </strong>and<strong> Waters Corporation / HPLC Symposium </strong>and technical note by<strong> KNAUER!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Intact Protein Analysis in Plasma Using Automated 2D-LC/TOF with Multiple Heart-Cutting</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_intact_protein_mhc_2d_lc_tof_wp_679_asms_2026_en_agilent_edb54f4854.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Direct intact and top-down protein analysis enables comprehensive characterization of proteoforms, post-translational modifications, and fragments that are often lost in bottom-up workflows.1 However, intact mass analysis in complex biological matrices such as whole plasma is challenged by extreme dynamic range and ion suppression, commonly necessitating affinity-based enrichment.&nbsp;</p><p>Here, we demonstrate a non-affinity, orthogonal separation strategy using a 2D-LC/TOF workflow with multi-heart-cutting for native intact protein analysis directly in plasma. Therapeutic monoclonal antibodies (mAbs) trastuzumab and antibody-drug conjugate T-DM1, were used as model systems to evaluate performance.&nbsp;</p><p>The workflow employs an Agilent 1290 Infinity III 2D-LC system for orthogonal separations and precise multiple heart-cutting, coupled to a high-resolution, high-sensitivity Agilent 6230C Accurate Mass TOF LC/MS System.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III+2D-LC"><strong>Agilent 1290 Infinity III 2D-LC system </strong></a>comprised the following modules:&nbsp;<ul><li>1290 Infinity III Bio Multisampler with Thermostat (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B) with Max-Light cartridge cell (G7117-60020)&nbsp;</li><li>1260 Infinity III Bio Flexible Pump (G7131C)&nbsp;</li><li>1290 Infinity III Bio High-speed Pump (G7132A)&nbsp;</li><li>1290 Infinity Valve Drive (G1170A) with 2D-LC ASM Valve (G5643B)&nbsp;</li><li>Two 1290 Infinity III Valve Drives (G1170A) with multiple heart-cutting valves (G5642-64000) equipped with 40 µL Bio loops&nbsp;</li><li>1290 Infinity III Valve Drive (G1170A) with a 2- position/6-port valve (G4231A) (used as a diverter valve)&nbsp;</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6230C"><strong>Agilent 6230C Accurate Mass TOF LC/MS System</strong></a></li></ul><h5><strong>LC/MS Analysis&nbsp;</strong></h5><p>Data acquisition and analysis were carried out using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+acquisition"><strong>MassHunter acquisition</strong></a> (12.2), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=BioConfirm"><strong>BioConfirm</strong></a> (12.1) and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+Qualitative+Analysis"><strong>MassHunter Qualitative Analysis </strong></a>(13.0) software.</p><h4><strong>Conclusions</strong></h4><ul><li>Agilent 2D-LC enables direct native MS analysis of intact proteins from plasma without affinity-based enrichment.&nbsp;</li><li>UV-guided and multiple heart-cutting simplify 2D-LC method development and provide high-resolution isolation of native proteins from complex biological matrices such as plasma.&nbsp;</li><li>The Agilent 6230C Accurate Mass TOF delivers high sensitivity and excellent mass accuracy, enabling confident intact protein characterization under native conditions.</li></ul><h3><strong>2. KNAUER: Mind the pressure in preparative up-scaling – effects of particle size and solvent composition on back pressure</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0051_knauer_mind_the_pressure_in_preparative_up_scaling_effects_of_particle_size_and_solvent_comp_12e49884d3.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The back pressure of a chromatographic system is generated by the system components, the capillaries, the column, the solvent, and the applied flow rate. Each system has a maximum pressure which it can sustain, and therefore the pressure must be monitored as it is a limiting factor during method development. The back pressure generated by a column is dependent on the particle size and its length. The development of a preparative method is ideally performed on an analytical scale and after optimization the method is then scaled up to preparative scale.</p><p>As preparative HPLC systems normally have lower maximum pressures, it is important to keep the generated pressure in the range of the later preparative system.&nbsp;</p><p>The larger the particles of the column material are, the lower will be the generated back pressure. With increasing particle size, the resolution is decreasing at the same time. Depending on the separation task, the resolution can be a critical point, and it is important to find the right compromise between resolution and backpressure. Additionally, larger particles are significantly less price intensive, which is another factor to be considered especially for large scale purifications. Often solvent mixtures of water with acetonitrile, methanol or ethanol are used for reverse phased methods. Depending on the organic solvent composition, the generated back pressure differs significantly which could be reduced by changing the solvent composition slightly or the organic solvent if possible. The back pressure generated by the solvent will not or only slightly change during the up-scaling process.&nbsp;</p><p>Here, the influence of particle size, flow rate and solvent composition on the back pressure was evaluated. Further, a practical scale-up was performed to evaluate the values obtained on analytical scale to those in larger scale.</p><h4><strong>User Tips:&nbsp;</strong></h4><ul><li>What is the finale scale you want to operate later?&nbsp;</li><li>What are the pressure limitations of the preparative system?&nbsp;</li><li>Be aware of the solvent back pressure&nbsp;</li><li>Consider particle size not only for best separation&nbsp;</li><li>Consider the flow rate</li></ul><h4><strong>MATERIAL AND METHODS&nbsp;</strong></h4><ul><li><strong>Pump: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2372"><strong>AZURA® P 6.1LHPG</strong></a>, 10 ml, sst&nbsp;</li><li><strong>Autosampler: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2375"><strong>AZURA AS 6.1L</strong></a></li><li><strong>Detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2423"><strong>UV AZURA DAD 2.1L&nbsp;</strong></a></li><li><strong>Flow cell: </strong>Pressure proof 10 mm, 10 µl, 300 bar&nbsp;</li><li><strong>Thermostat:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2376"><strong>CT2.1&nbsp;</strong></a></li><li><strong>Columns:&nbsp;</strong><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1633"><strong>Eurospher II 100-5 C18, 150 x 4.6 mm&nbsp;</strong></a></li><li>Eurospher II 100-10 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-15 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-20/45 C18, 150 x 4.6 mm &nbsp;</li></ul></li><li><strong>Software&nbsp;</strong><ul><li>ClarityChrom 10.1&nbsp;</li><li>ClarityChrom 10.1 PDA extension</li></ul></li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>The back pressures of four C18 150 x 4.6 mm columns, which differed only in particle size were measured and compared at different flow rates and solvent compositions. As expected, the 5 µm column showed the highest and the 20/45 µm column the lowest back pressure at all tested conditions. The pressure curves of ethanol and methanol gradients were similar, with a maximum pressure at approximately 50% solvent. The pressure curve of the acetonitrile gradient differed from the other two, showing the highest pressure at a lower organic solvent content of approximately 35%, after which the pressure dropped. Ethanol showed the highest pressure, followed by methanol and then acetonitrile. Determining the column back pressure at an analytical scale is an important tool during preparative method development. Ideally, if a linear scale up from the analytical to the preparative scale is conducted, the column back pressure should stay the same. This helps to predict if the method can be scaled up to the desired size in terms of back pressure.</p><h3><strong>3. Shimadzu / ASMS: Enhanced Quantification of Emerging UV Filter Contaminants in Drinking Water Using Optimized LC-MS/MS Technique</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_121_Enhanced_Quantification_of_Emerging_UV_Filter_Contaminants_in_Drinking_Water_Using_Optimized_LC_MSMS_Technique_e733c64828.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Ultraviolet (UV) filter chemicals from sunscreens and personal care products are increasingly recognized as emerging contaminants of concern in drinking water1 . Their persistence and reported biological effects highlight the need for sensitive and reliable monitoring approaches2 . The objectives were to optimize quantitation to achieve ppt level detection without offline extraction and assess the occurrence of these compounds in multiple drinking water matrices. In this study, 20 UV filters were analyzed using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060RX"><strong>Shimadzu LCMS8060RX triple quadrupole mass spectrometer.</strong></a></p><h4><strong>Conclusion</strong></h4><p>A highly sensitive, precise, and robust direct injection LC–MS/MS method enables sub-ppt to high-ppb quantification of 20 UV filters in drinking water. Overall, the highly sensitive and robust Shimadzu LCMS-8060RX Triple Quadrupole Mass Spectrometer combined with this extraction-free workflow provides a simple, reliable, and high-throughput approach for quantifying emerging UV filters at ng/L concentrations in drinking water.</p><h3><strong>4. Thermo Fisher Scientific / HPLC Symposium: Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004752_hplc_vanquish_amplify_metal_sensitive_analyte_analysis_po004752_na_en_ff99cfde49.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate LC-UV analysis of large RNA and oligonucleotide therapeutics requires highly inert chromatographic flow paths because interactions between the negatively charged phosphodiester backbone and metallic surfaces can cause analyte loss, peak broadening, and reduced quantitative sensitivity, particularly at low concentration.&nbsp;</p><p>Here, an inert UHPLC system – Vanquish Amplify system, incorporating inert-coated flow path components was evaluated against a biocompatible UHPLC configuration using MP35N alloy capillaries and additionally against a commercially available inert UHPLC platform. Large RNA fragments of 500 and 5000 nucleotides were analyzed by IP-RPLC to assess the difference between these systems. To further investigate flow path interactions, adenosine 5′- (α,β-methylene) diphosphate (AMPcP), a metal-sensitive analyte, was analyzed alongside adenosine, a noninteracting reference compound.</p><h5><strong>Material and Methods</strong></h5><ul><li><strong>Instrumentation: </strong>Thermo Scientific Vanquish Amplify UHPLC system</li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=SurePac+Oligo"><strong>Thermo Scientific SurePac Oligo RP MDi HPLC column</strong></a>,<br>2.5 µm 2.1 X 50 mm (P/N: 43712-052132</li><li><strong>Data Analysis: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>The Thermo Scientific Chromeleon Software 7.3.2 MUc Chromatography Data System (CDS)</strong></a> was used for all data acquisition and analysis.</li></ul><h4><strong>Conclusions&nbsp;</strong></h4><p>Using AMPcP with the Vanquish Amplify system demonstrated up to a twofold increase in sensitivity compared to systems with MP35N fluidics under conditions where metal interactions are most pronounced&nbsp;</p><p>For large RNA, the Vanquish Amplify UHPLC system delivered 1.25–1.35× higher signal intensity compared to systems with MP35N fluidics, enabling reduced sample consumption and improved quantitative reliability.&nbsp;</p><p>The Vanquish Amplify UHPLC system demonstrated comparable system inertness under the tested conditions for larger RNA fragments (5,000 nt) and slightly better system inertness for smaller fragments (500 nt) in this study than the MaxPeak HPS containing system, based on peak height and area comparisons using an external UV detector</p><h3><strong>5. Waters Corporation / HPLC Symposium: Development and Transfer of Quantitative Analytical Methods using Universal Detectors</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009493_8cbe4c3cc2.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>Universal detectors such as <strong>Charged Aerosol Detection (CAD)</strong> and <strong>Evaporative Light Scattering Detection (ELSD)</strong> provide valuable alternatives to traditional LC detectors including UV, fluorescence, and mass spectrometry, particularly for analytes lacking chromophores. Although both detectors are suitable for quantitative analysis, they rely on different detection principles—CAD measures the electrical charge transferred to aerosol particles, whereas ELSD detects light scattered by dried analyte particles. Because both detectors exhibit non-linear responses, quantitative methods typically require calibration models such as log-log linear or quadratic regression rather than conventional linear calibration. The poster discusses key considerations for developing robust quantitative methods and highlights the challenges associated with transferring methods between different universal detection platforms.</p><p>To demonstrate detector transferability, the authors migrated a <strong>HILIC method for sugar analysis in fruit juice</strong> from ELSD to CAD. The separation was performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/306"><strong>Waters Arc HPLC System</strong></a> equipped with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=XBridge+BEH+Amide"><strong>XBridge BEH Amide</strong></a><strong> (4.6 × 150 mm, 2.5 μm)</strong> column using a water/acetonitrile gradient containing triethylamine. While the chromatographic conditions remained unchanged, CAD detector parameters were optimized. Both detectors produced highly comparable quantitative performance across a calibration range of <strong>50–1000 μg/mL</strong>, achieving calibration coefficients (R²) above <strong>0.995</strong>, area RSD values below <strong>1.5%</strong>, and nearly identical concentrations of fructose, sorbitol, glucose, and sucrose in an apple juice sample. These results demonstrate that quantitative HILIC methods can be successfully transferred between ELSD and CAD with only detector-specific optimization.</p><p>The poster also evaluates method transfer between different CAD platforms using the <strong>USP Deoxycholic Acid assay</strong>. The method was implemented on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>Waters ACQUITY UPLC H-Class PLUS System</strong></a> with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge BEH C18</strong></a><strong> (4.6 × 150 mm, 3.5 μm)</strong> column and compared against a competing LC/CAD system. After scaling the method according to <strong>USP Chapter &lt;621&gt;</strong>, both systems met all USP system suitability requirements, including repeatability and signal-to-noise criteria. Quantitative results for deoxycholic acid and related impurities were virtually identical, confirming that validated CAD methods can be reliably transferred between different vendors' LC/CAD platforms without compromising analytical performance.</p><p>Overall, the study demonstrates that both ELSD and CAD are robust universal detection techniques for quantitative liquid chromatography. While method transfer between identical detector types is generally straightforward, successful migration between ELSD and CAD—or between different CAD systems—requires careful optimization of detector parameters while preserving chromatographic conditions. The presented workflows illustrate that with appropriate calibration and detector tuning, laboratories can achieve equivalent sensitivity, precision, and quantitative accuracy, facilitating method standardization and transfer across analytical platforms.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 29, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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SciY/Mestrelab Research

Waters Corporation

<h4><strong>Key Learning Points</strong></h4><ul><li>Understand how backpressure is measured in LC instruments.</li><li>Learn why backpressure is important to measure in the system and column.</li><li>Explore how different dimensions of column hardware and system tubing can influence overall backpressure.</li><li>Examine how different mobile phases and flow rates can impact backpressures.</li><li>Find out what different types of sample matrices can do to backpressure and how to mitigate.</li><li>Listen in to tips to troubleshoot the root cause of backpressure issues.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any liquid chromatography users who are looking to understand the factors that can impact backpressure and how to mitigate potential backpressure problems.</li></ul><blockquote><p><strong>Speaker:</strong> Koen Askamp (Senior Technical Specialist, Phenomenex)</p><p>&nbsp;Koen Askamp is a Senior Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.&nbsp;</p></blockquote>

Phenomenex: Under Pressure - Mastering Backpressure in HPLC

Under Pressure: Mastering Backpressure in HPLC

Learn to manage HPLC backpressure. Discover how mobile phases, column hardware, and sample matrices impact system pressure with Phenomenex experts

Mastering HPLC Backpressure: Troubleshooting & Tips

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>The rapid expansion of complex biotherapeutic modalities such as RNA-based therapies is pushing conventional LC–MS workflows beyond their limits. Even though there has been great progress in oligonucleotide drug development and approval there is still significant regulatory and analytical challenges in this field. Characterization guidelines are still under development and must account for unique challenges. Regarding impurity characterization, most impurities exist as mixture of closely related molecules and even worse, many impurities coelute with the active molecule. Many existing approaches struggle to deliver reliable separation and high-quality MS data to adequately resolve those impurities. Challenges such as good chromatographic resolution, metal adduct formation, and inconsistent MS data quality are increasingly impacting characterization workflows.</strong></p><p>In this webinar, we introduce a next-generation LC–MS strategy designed to address these gaps. By combining advanced reversed-phase chromatography with an inert UHPLC flow path, this workflow improves separation of challenging species, minimizes metal adducts, and delivers more consistent, more interpretable MS data across diverse modalities.</p><p>Using the Thermo Scientific™ SurePac™ RP MDi™ column on the Thermo Scientific™ Vanquish™ Amplify UHPLC system coupled with Orbitrap™ MS, we will demonstrate how this approach enhances analytical performance in real-world applications, including impurity profiling for small single stranded oligonucleotide therapeutics, such as antisense oligonucleotides (ASOs), using a DoE-driven approach.</p><h4><strong>Learning Objectives:</strong></h4><ul><li>Understand how advanced LC-MS workflows support characterization across new modalities such as ASOs, including detection of critical quality attributes such as the confirmation of the primary structure together with the analysis of the 5′ and 3′ termini, and any site-specific modification and the detailed analysis of product-related impurities.</li><li>Evaluate how inert column technologies and UHPLC systems improve reproducibility, reduce sample interaction, minimize metal adducts and enhance robustness for complex biotherapeutic workflows.</li><li>Apply design of experiments (DoE) driven method optimization and LC–MS approaches for impurity profiling, and comprehensive characterization.</li></ul><p>Join us to see how this workflow can unlock deeper characterization and more confident results across today’s most demanding biotherapeutic modalities.</p><blockquote><p><strong>Presenter: </strong>Mauro De Pra, PhD &nbsp;(Product Marketing Manager, Thermo Fisher Scientific)</p><p>Mauro De Pra, who has been with Thermo Fisher Scientific since 2010, has held roles spanning across product development, application development, technical support, and product marketing. His work focuses on HPLC and advanced separation technologies, supporting the development and commercialization of innovative LC solutions. Mauro has authored multiple peer-reviewed publications in the field of separation sciences and brings extensive experience translating analytical challenges into practical solutions for pharmaceutical and biopharmaceutical applications. Before joining Thermo Fisher Scientific, he worked as an Analytical Development Scientist in the Pharmaceutical industry. Mauro holds a Ph.D. in Chemistry from the University of Amsterdam.</p></blockquote><blockquote><p><strong>Presenter: </strong>Silvia Millán-Martín, PhD (Senior Research Scientist, National Institute for Bioprocessing Research and Training (NIBRT))</p><p>Silvia is a Senior Research Scientist at the National Institute for Bioprocessing Research and Training (NIBRT) in Dublin, Ireland. She collaborates with Thermo Fisher Scientific to develop innovative applications for the characterization of biopharmaceuticals. Her work encompasses a broad range of chromatographic techniques, ensuring comprehensive analytical solutions for the scientific and biopharmaceutical industries. Thanks to her extensive background in analytical chemistry and her dedication to advancing biopharmaceutical characterization, Sylvia’s a leading expert in her field. During the last few years, her work has focused on the Multi-Attribute Method (MAM) approach and the application of advanced LC-MS platforms to support the development of new therapeutic modalities such as oligonucleotide-based therapies.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Characterizing Complex Biotherapeutics Based on New Modalities - Uncover new LC-MS Workflow Strategies

Characterizing Complex Biotherapeutics Based on New Modalities: Uncover new LC-MS Workflow Strategies

Master oligonucleotide impurity profiling. Discover how inert column technology, DoE-driven workflows, and Orbitrap MS enhance biotherapeutic analysis.

Advanced LC-MS Workflows for Oligonucleotide Analysis

<p><strong>As regulatory expectations around per- and polyfluoroalkyl substances (PFAS) continue to evolve, pharmaceutical laboratories are under increasing pressure to detect and quantify PFAS at ultra-trace levels across complex sample matrices.</strong></p><p>Join this live webinar to explore analytical approaches for sensitive, selective, and reproducible PFAS analysis in pharmaceutical applications.</p><p>Drawing on representative workflows and quantitative performance data, this session will highlight strategies for PFAS monitoring in active pharmaceutical ingredients (APIs) and pharmaceutical packaging materials using the SCIEX 7500+ system.</p><p>Attendees will gain insight into method development considerations, contamination control strategies, chromatographic separation approaches, and quantitative performance for broad PFAS panels in pharmaceutical workflows.</p><h4><strong>What you will learn</strong></h4><ul><li>Key analytical challenges associated with PFAS monitoring in pharmaceutical workflows, including contamination control and complex sample matrices</li><li>Approaches for sensitive and selective PFAS quantitation in APIs and pharmaceutical packaging materials using LC-MS/MS</li><li>Chromatographic and workflow strategies to improve PFAS separation, reproducibility, and quantitative confidence</li><li>Quantitative performance results, including LOQs as low as 0.005 ng/mL, broad linear dynamic range, and recovery performance across multi-compound PFAS panels</li></ul><blockquote><p><strong>Speaker: </strong>Dr. Cathy Lane</p><p>Joined SCIEX in 2006 as a Support Specialist and in 2021 took up the role of EMEAI Applications Lead for Pharma &amp; Peptide Quant.</p><p>She has more than 20 years’ experience in the development of LC/MS methods across multiple application areas, and specializes in workflows for peptide quantification.</p><p>She obtained her PhD in mass spectrometry-based proteomics from the University of London School of Pharmacy in 2004</p></blockquote>

PFAS in pharmaceuticals

Discover analytical strategies for sensitive PFAS monitoring in APIs and pharmaceutical packaging using LC-MS/MS and advanced contamination control

Sensitive and robust PFAS quantitation in APIs

<p><strong>This webinar is co-hosted by SciY (a Bruker company) and Novalix, who have jointly developed and will deliver the content of the session.</strong></p><p><strong>“Confused” (or perhaps overwhelmed) when hearing semantics, ontologies, schema, knowledge graphs and digital twins? This webinar is different. As experimental scientists, Julien Marin (Novalix) and Anna Codina (SciY) will focus on how automation and AI-powered experimentation are applied in day-to-day experimental settings, from discovery to manufacturing.</strong></p><p>Through concrete examples, we will show how labs are moving beyond isolated automation toward connected, end‑to‑end workflows: integrating data directly from instruments, enriching it with scientific context, applying automated analysis, and using AI to guide the next experimental step.</p><p>We will walk through practical scenarios such as reaction screening, structure verification, and closed loop experimentation, as well as their extension into AI piloted self-contained distributed manufacturing. The emphasis is not on theory, but on what works in practice: what has been built, what has been tested, and what delivers value today.</p><p>Attendees will come away with a clear understanding of how to move from fragmented tools and manual workflows to connected experimentation systems where data, automation, and AI are combined to accelerate decisions.</p><h4><strong>Learning Points</strong></h4><ul><li>What real workflow automation looks like beyond concept diagrams</li><li>How to connect instruments, data, and systems into end-to-end experimental workflows</li><li>How experimental data is captured, structured, and reused to enable automation and AI</li><li>How closed loop experimentation works in practice</li><li>How AI can move from post data analysis to actively guiding experiments and processes</li><li>How these approaches extend from R&amp;D into process optimisation and continuous manufacturing</li></ul><h4><strong>Who should attend</strong></h4><p>This webinar is ideal for professionals who want to move from concepts to real implementation:</p><ul><li>Scientists and lab leads working with automation or high throughput experimentation</li><li>R&amp;D and digital lab leaders looking to turn strategy into execution</li><li>Automation, robotics, and workflow engineering teams</li><li>Data, informatics, and AI teams supporting experimental workflows</li><li>Process development, PAT, and manufacturing innovation teams</li><li>CRO/CDMO leaders interested in scalable, connected experimental platforms</li></ul><blockquote><p><strong>Presenter:</strong> Julien Marin, PhD (Chief Operating Officer, Novalix)</p><p>Julien Marin is Senior Vice President and Chief Operating Officer at Novalix. He joined the company in 2006, and, over two decades, has held progressive leadership roles, including Head of Synthetic Chemistry, Business Unit Director for Chemistry, and Director of Research Services.</p><p>Throughout the first part of his career, Julien oversaw collaborations in research services, primarily in drug discovery, within the pharmaceutical sector, but also in agrochemicals, cosmetics, and fine chemicals. He has led multidisciplinary R&amp;D teams in Belgium, France, and Spain, deployed new services, and played a key role in talent development. Currently, Julien is contributing to developing the Novalix strategic roadmap and ensuring its deployment and implementation across the company.</p><p>Julien holds a PhD in Chemistry from Strasbourg University and a Master’s degree in Strategic Business Unit Management from HEC Paris. He completed postdoctoral training with S. Hanessian at the University of Montreal, where he worked on various projects in asymmetric synthesis and medicinal chemistry, including a drug discovery program with a pharmaceutical company.</p></blockquote><blockquote><p><strong>Presenter:</strong> Anna Codina, Ph.D. (Senior Director Strategy and Business Development, SciY)</p><p>Anna Codina is Senior Director of Strategy and Business Development at SciY, where she focuses on enabling data-driven orchestration across the pharmaceutical value chain. With a background in experimental science, analytical development, and digital innovation, she works at the intersection of data, automation, and AI to accelerate scientific workflows and decision-making.</p><p>Anna holds a Ph.D. in protein NMR from the University of Barcelona and completed postdoctoral research at the MRC Laboratory of Molecular Biology in Cambridge. She spent seven years in Analytical R&amp;D at Pfizer, specializing in reaction monitoring, impurity characterization, and regulatory submissions, and was recognized with a Pfizer Worldwide Achievement Award for implementing NMR-based reaction monitoring in an open-access environment.</p><p>Since joining Bruker, Anna has held multiple leadership roles across applications, product management, and biopharma business development and market management. In her current role at SciY, she drives strategy for vendor-agnostic data platforms and digital solutions that connect instruments, robotics, and enterprise systems enabling orchestration, AI-powered experimentation and scalable workflows across R&amp;D, CROs/CDMOs, and manufacturing.</p></blockquote>

SciY: No More Boxes on Slides. Examples of Workflow Orchestration and Their Impact from R&D to Production on Demand

No More Boxes on Slides. Examples of Workflow Orchestration and Their Impact from R&D to Production on Demand

Learn how SciY and Novalix apply AI-powered automation to experimental workflows. Move from isolated tools to connected, closed-loop R&D systems

Connected Experimentation: Lab Automation and AI in Practice

<p><strong>Food fortification has moved from a public health aspiration to a regulatory reality in India. FSSAI's fortification standards now cover key staples, and the +F logo that consumers recognise comes with specific analytical requirements that food businesses must meet.</strong></p><p>Consider rice fortification. Adding iron, folic acid, and Vitamin B12 to rice kernels is one thing. Verifying that those nutrients are present at the right levels in the finished product requires rigorous analytical testing using techniques like ICP-MS for mineral quantification and LC/MS for fat-soluble and water-soluble vitamins. Without that data, a fortification claim is just a label.</p><p>Milk fortification tells a similar story. Vitamin A and Vitamin D are routinely added to milk across India, but confirming their stability and concentration through the supply chain requires HPLC-based methods that can detect these micronutrients accurately even in complex dairy matrices. This webinar brings together regulatory and scientific perspectives to give food professionals a clear picture of where India's fortification programme stands today, what the analytical standards demand, and where the industry needs to sharpen its approach.</p><h4><strong>Key Learning Outcomes:</strong></h4><ul><li>Understand the current FSSAI regulatory framework for food fortification in India, including recent updates to standards across staple categories</li><li>Learn about the analytical methods used to measure vitamins and minerals in fortified foods, including LC/MS, HPLC, and ICP-MS, and the challenges specific to different food matrices</li><li>Get clarity on what FSSAI's +F endorsement requirements mean for testing and documentation</li><li>Identify common gaps in fortification testing programmes and how to address them</li><li>Understand what regulators expect when manufacturers make fortification claims on labels</li></ul><h4><strong>Who Should Attend:</strong>&nbsp;</h4><p>This webinar is for professionals in the Indian food industry involved in food safety, quality, or product development, specifically:</p><p>QA and QC managers responsible for fortified food categories, R&amp;D scientists working on product formulation and fortification, regulatory affairs professionals managing FSSAI compliance, lab heads and analytical chemists working on food testing, food safety officers in manufacturing facilities, procurement and technical teams working with fortification premix suppliers.</p><blockquote><p><strong>Presenter: </strong>Dr. Dinesh Kumar Singh (Assistant Professor, Nanoscience and Nanotechnology, NIFTEM-K, India)</p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Dr. Vikrant Goel (Application Scientist, Agilent Technologies India Pvt. Ltd.)</p><p><span>Vikrant comes with an extensive 19 years of experience working in QA/QC, R&amp;D and Analytical roles and an expert in various LCMS technologies. His solution-based approach has been well accepted among LCMS fraternity from Pharma and Applied market segments, since 2004. He holds an M.tech in Biotechnology, PG Dip in Bioinformatics &amp; B. Pharm. He joined Agilent in 2010, as a Product specialist for LC/MS, after having worked with ILS Bioservices &amp; LabIndia Instruments. His passion towards solution, brought him back to the Application role, 6 years ago.</span></p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Dr. Vinay Jain (Application Engineer, Agilent Technologies, India)</p><p>Dr. Vinay Jain is working as the CoE Manager and Country Food Workflow Commercialization Lead at Agilent Technologies International. He holds a Ph.D. in Analytical Chemistry and has more than 33 years of analytical and research experience in spectroscopic and mass spectrometry techniques.</p><p>His specialization includes the development and validation of new analytical methods in accordance with regulatory guidelines. He has completed over 28 research projects with various national and international research and regulatory organizations. He holds two patents, has published 59 research papers, contributed two chapters to international handbooks, and authored 15 Agilent application notes. He received the Agilent TechInnovation Award in 2020 and 2022 for providing end-to-end workflow solutions. One of his innovations on LC-ICPMS was selected for the Winter Plasma Conference, and two were selected for the European Winter Plasma Conference for poster presentations.&nbsp;</p></blockquote>

Agilent Technologies: Food Fortification, Measured - The Analytical Testing Behind the Standards

Food Fortification, Measured: The Analytical Testing Behind the Standards

Discover analytical workflows for food fortification. Learn how to quantify vitamins and minerals using LC-MS, HPLC, and ICP-MS for FSSAI compliance

Food Fortification Testing: Analytical Methods & FSSAI

<p><strong>Metabolomics is a core pillar of modern systems biology, offering deep insights into the dynamic metabolic phenotypes that underpin human health and disease. Realizing its full potential requires generating high-quality and reproducible representations of complex metabolomes and enabling accurate comparison among them.</strong></p><p>In this SelectScience® TechTalk, Dr. Matthew Lewis, Bruker Daltonics' Vice President of Metabolomics &amp; Lipidomics, makes the case that metabolomics advances when quality is in focus. With the timsMetabo™ and its 4D data richness as the foundation, he examines what it takes to achieve confidence at scale, employing advanced quality control to monitor measurement precision, annotation scoring to ensure accurate connection to biochemical processes, and hybrid workflows that allow quantitation and discovery to thrive on the same platform.</p><p>The timsMetabo's multidimensional, AI-ready data and the digital metabolome archive turns the question from: Have I ever seen this before? into an executable query, using MetaboScape to connect spectra and annotations across distinct studies. Dr. Lewis sets out how this connected future is taking shape, and why Bruker, as a full solutions provider, is best positioned to deliver it.</p><p>By registering, you will learn how this connected approach reduces re‑runs, strengthens data comparability across studies, and equips you to generate the robust, AI‑ready datasets that accelerate discovery and support more reliable systems‑level insights.</p><p><strong>Certificate of attendance</strong><br>If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.</p><p><strong>TechTalk details</strong></p><ul><li>Cost: Free to attend</li><li>Location: Online</li><li>Duration: 20 minutes</li></ul><p><i>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.</i></p><blockquote><p><strong>Speaker: </strong>Dr. Matthew Lewis (Vice President, Metabolomics &amp; Lipidomics, Bruker Daltonics)</p><p>For the past 25 years, Dr. Matthew Lewis has pursued his interests in better understanding biochemical processes and improving the analytical tools and techniques used to measure them. At Imperial College London, he served as the chief operating officer of the UK's National Phenome Centre and head of the academic section of Bioanalytical Chemistry in the Faculty of Medicine. Here, he worked to advance the understanding of human disease phenotypes using advanced bioanalytical and data analysis techniques for metabolic profiling at a previously unprecedented scale.</p><p>Lewis transitioned to industry in 2022 to more directly further the development of research-enabling solutions through his role at Bruker Daltonics. He takes great pleasure in extensive engagement in interdisciplinary team science and large-scale scientific collaborations, supporting advancements in the fields of metabolomics and lipidomics.</p></blockquote>

Bruker: Building confidence at scale in 4D‑Metabolomics with data quality, depth and connectivity in focus

Building confidence at scale in 4D‑Metabolomics with data quality, depth and connectivity in focus

Discover Bruker's 4D-metabolomics workflows. Learn how timsMetabo delivers high-quality, AI-ready datasets and robust annotations via MetaboScape.

Building Confidence at Scale in 4D-Metabolomics

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Sterility testing remains a critical component of batch release and overall sterility assurance. This webinar provides a practical overview of compendial approaches, linking method selection, media considerations, and workflow execution to product-specific needs. Participants will explore testing strategies aligned with USP General Chapter &lt;71&gt; Sterility Testing alongside modern, rapid approaches, with emphasis on risk-based decision-making, operational efficiency, and alignment with key microbiological chapters to support robust, compliant sterility programs.</strong></p><h5><strong>Learning Objectives:</strong></h5><ul><li>Explain sterility testing within a holistic sterility assurance strategy</li><li>Differentiate methods based on product characteristics</li><li>Select appropriate media and evaluate bottled formats</li><li>Identify critical controls in traditional sterility testing workflows</li><li>Describe principles and benefits of rapid sterility methods</li></ul><blockquote><p><strong>Presenter: </strong>Leslie Furr, Principal Scientist, United States Pharmacopeial Convention (USP)</p><p>Leslie Furr is a Principal Scientist in the Microbiology Unit at the United States Pharmacopeial Convention (USP). She has been with USP since 2018, serving as a documentary standards scientist for the Microbiology Expert Committee (MEC). In this role, she contributes to the development and revision of microbiology standards and guidance for global stakeholders. Leslie also serves as the primary liaison for the Endotoxins and Pyrogens Subcommittee, an MEC subcommittee focused on documentary standards for this topic.</p><p>She holds a B.S. in Microbiology from the University of North Carolina at Charlotte and an M.S. in Pharmaceutical Sciences from the University of Florida. Leslie has been certified by the American Society for Microbiology as a Specialist Microbiologist in Pharmaceutical and Medical Devices since 2012.</p><p>Prior to joining USP, she spent 14 years in the pharmaceutical industry as a Senior Microbiologist, focusing on method development, research and development, and manufacturing support.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Sterility testing remains a critical component of batch release and overall sterility assurance

Sterility testing remains a critical component of batch release and overall sterility assurance

Learn sterility testing strategies, compendial and rapid methods, media selection, and risk-based approaches for pharmaceutical quality assurance.

Sterility testing 

<div><p><strong>As mass spectrometry systems continue to advance in analytical sensitivity, robustness, and ease of use, some may question whether liquid chromatography (LC) remains a critical component of the clinical LC-MS/MS workflow. Is LC simply an inlet to the mass spectrometer - or does it play a more fundamental role in analytical performance?</strong></p><p>In this webinar, we will challenge the notion that LC is "just an inlet" and explore why chromatographic separation remains essential for generating reliable, high-quality clinical results. Attendees will learn how effective LC improves assay sensitivity, enhances reproducibility, minimizes matrix effects, and reduces analytical interferences that can compromise confidence in clinical results.</p><p>Focusing on Waters UPLC technology, including the <strong>ACQUITY UPLC I-Class PLUS System</strong>, we will examine how advanced chromatographic performance contributes to improved method robustness, consistent retention times, reduced rework, and greater operational efficiency in routine clinical laboratories.</p><p>Join us to discover why LC and MS deliver their greatest value as an integrated solution - and why UPLC remains a foundational element of successful clinical LC-MS/MS workflows.</p><h4><strong>What You'll Learn:</strong></h4><ul><li>Why chromatographic separation remains critical despite advances in MS technology</li><li>How LC impacts analytical sensitivity, reproducibility and assay robustness</li><li>Strategies to reduce matrix effects and analytical interferences</li><li>The benefits of UPLC for routine clinical laboratory operations</li><li>Why integrated LC-MS/MS solutions deliver superior analytical performance and workflow efficiency compared with viewing LC as merely an inlet to the mass spectrometer</li></ul><blockquote><p><strong>Presenter:</strong> Martien van der Meijden (Principal Scientist, Waters Advanced Diagnostics)</p><p>Martien van der Meijden, Principal Scientist in Waters Advanced Diagnostics Business Unit, brings deep expertise in LC-MS method development and troubleshooting. Based in the Netherlands and part of Waters since 2018, Martien has authored a substantial portion of Waters training content and has supported forensic and clinical labs across Europe with hands-on guidance.</p><p>He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives.</p></blockquote></div>

Waters Corporation: Does Chromatography Still Matter? The Critical Role of UPLC in Clinical LC-MS/MS Performance

Does Chromatography Still Matter? The Critical Role of UPLC in Clinical LC-MS/MS Performance

Learn how UPLC enhances clinical LC-MS/MS by improving sensitivity, robustness, reproducibility, and reducing matrix effects and interferences.

Role of UPLC in Clinical LC-MS/MS Performance

<h4><strong>The Problem&nbsp;</strong></h4><p>Buffer exchange and sample concentration stand between you and everything else you need to get done — but traditional methods are slow, manual, and impossible to fit into an automated workflow. Just a few samples can keep you tied up for hours and as the throughput goes up, buffer exchange and concentration become the bottleneck of therapeutic development.</p><h4><strong>The Solution&nbsp;</strong></h4><p>Lil' Tuna is the first fully automated, plate-based benchtop buffer exchange system built for proteins, nucleic acids, and gene therapy vectors at any scale. Lil’ Tuna concentrates or exchanges in 96-well or 24-well Unfilter plates for high throughput or up to 6 individual samples in Unas — all with ≥96% recovery. Its pressure-based ultrafiltration/diafiltration approach keeps things flowing efficiently, tracks sample volumes and accurately adds buffer throughout, handling every step of the exchange cycle automatically from start to finish.</p><h4><strong>The Proof&nbsp;</strong></h4><p>In this seminar, you’ll see how easy it is to run buffer exchange or concentration experiments on Lil’ Tuna. Whether working with a full plate of 96 samples or with just a few, the experiment setup is straightforward. Scope the different types of experiments: a buffer exchange with optional concentration or simply concentration by itself. Finally, take a peek at how Lil’ Tuna packs all it needs to integrate into automation workflows with universal API software, a sample stage designed for easy access and simple template import functions.&nbsp;<br>&nbsp;</p><blockquote><p><strong>Presenter:&nbsp;</strong>Brian Haldeman<strong> </strong>(Product Manager, Lil' Tuna, Unchained Labs)</p><p>Brian Haldeman is Product Manager for Lil' Tuna at Unchained Labs. Prior to this role, he was a Field Application Scientist supporting biophysical characterization of proteins and viral vectors. Brian received his Ph.D. in Biochemistry from the University of Nevada, Reno where he studied the mechanics of muscle contraction. His research utilized single-molecule microscopy, pre-steady-state kinetics, protein affinity and binding characterization, and protein isolation techniques.</p></blockquote>

Unchained Labs: Get hooked on automated buffer exchange with Lil’ Tuna

Get hooked on automated buffer exchange with Lil’ Tuna

In this seminar, you’ll see how easy it is to run buffer exchange or concentration experiments on Lil’ Tuna. 

Automated buffer exchange with Lil’ Tuna

<p><strong>Understanding PFAS workflows means mastering their complexity. Each matrix has its own workflow, but they are all based on chemistry. This webinar will guide you through the entire process, from sample preparation to final analysis, highlighting how different matrices require different strategies but share common principles.</strong></p><p>Join us to discover practical tips and proven methods for obtaining robust and reproducible PFAS data with Restek.</p><h4><strong>Key learnings:</strong></h4><ul><li>Best practices for handling different matrices and preventing PFAS contamination using appropriate PFAS-free materials.&nbsp;</li><li>Expanded PFAS analysis approaches covering ultrashort-, short-, long-chain, and alternative compounds using advanced LC methods and polar columns.</li><li>Modern method development tools, including in silico modeling, to reduce lab workload and improve efficiency.</li></ul>

Restek Corporation: PFAS - Analysis of Per- and Polyfluorinated Alkyl Substances

PFAS - Analysis of Per- and Polyfluorinated Alkyl Substances

Join us to discover practical tips and proven methods for obtaining robust and reproducible PFAS data with Restek.

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