<div><p><strong>Two-dimensional liquid chromatography (2D-LC) was introduced to overcome the limited peak capacity of one-dimensional LC (1D-LC), which often fails with highly complex mixtures. By coupling two separation dimensions with distinct retention mechanisms, 2D-LC enhances resolution, selectivity, and analytical depth.</strong></p><p>Its effectiveness stems from orthogonality—the deliberate use of complementary mechanisms, such as reversed-phase and hydrophilic interaction chromatography (<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-hilic-chromatography"><strong>HILIC</strong></a>)—which maximizes peak capacity and enables the separation of compounds unresolved in 1D-LC.</p><p>2D-LC has become integral to diverse applications. In metabolomics and proteomics, it supports large-scale peptide, protein, and metabolite profiling. Pharmaceutical laboratories employ it for impurity profiling, stability studies, and regulatory testing, while environmental and food sciences use it to detect trace pollutants, pesticides, and other contaminants in complex matrices.</p><p>Recent advances, including integration with high-resolution mass spectrometry, automation, and AI-based data processing, have streamlined workflows and reduced labor intensity. Sustainability efforts, such as solvent recycling and miniaturization, further support its adoption. By combining precision, versatility, and scalability, 2D-LC represents a transformative platform for contemporary analytical science.</p></div><div><div><div><div><h3><strong>Principle and Working Mechanism of 2D-LC</strong></h3><p>Two-dimensional liquid chromatography (2D-LC) is based on the principle of orthogonality. This term refers to the degree of independence between two chromatographic separation mechanisms.</p><p>By selecting stationary and<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/mobile-phase-in-chromatography"><strong>mobile phases</strong></a> that exploit different chemical properties such as polarity, hydrophobicity, ionic charge, or molecular size, 2D chromatography achieves superior resolving power. This orthogonality ensures that analytes co-eluting in the first dimension can be effectively separated in the second.</p><p>Peak capacity is one of the key performance metrics in 2D liquid chromatography. It approximates the product of the separation capacity of each dimension. For instance, a first dimension with a capacity of 100 and a second of 150 produces an estimated combined peak capacity of 15,000. This exponential gain enables comprehensive profiling of complex mixtures and enhances analytical confidence.</p><p>The workflow of 2D chromatography is systematic and highly automated. It begins with sample injection, after that, analytes are separated in the first column. Modulation or fractionation then transfers portions of this effluent to the second column, either in real-time or via fraction collection.</p><p>The second-dimension separation employs steep gradients and high-pressure systems to achieve rapid resolution. Finally, detection is carried out using UV-Vis, fluorescence, or mass spectrometry, providing both qualitative and quantitative insights.</p><p>Modern 2D-LC platforms incorporate advanced software and automation, which helps them to enable reproducibility, efficient data processing, and minimal operator intervention.</p></div></div></div></div><div><div><div><div><h3><strong>Modes of 2D-LC Operation</strong></h3></div></div></div></div><div><p>Two-dimensional liquid chromatography (2D-LC) can be performed in different modes. Each of these modes is designed to balance resolution, analysis time, and sample coverage. Two majorly used approaches are comprehensive 2D-LC (LC×LC) and heart-cutting 2D-LC (LC–LC). Comprehensive mode provides full sample coverage and the highest resolution; in contrast, heart-cutting mode offers a targeted and efficient approach for specific analytes.</p></div><div><div><div><div><h4><strong>Comprehensive 2D-LC (LC×LC)</strong></h4></div></div></div></div><div><p>In comprehensive 2D-LC, every fraction eluting from the first dimension undergoes further separation in the second dimension. This all-encompassing approach is ideal for untargeted studies, including metabolomics, proteomics, environmental monitoring, and materials science, where sample complexity is extreme.</p><p>LC×LC demands rapid second-dimension separations, generally executed on short sub-2 µm <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50">lc-columns</a> under ultrahigh-pressure liquid chromatography (<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/liquid-chromatography/formats-techniques/lc-formats-uhplc-columns"><strong>UHPLC</strong></a>) conditions. Its strength lies in delivering maximum peak capacity and ensuring no compound is mis-analyzed. However, this method generates large datasets, making advanced data handling, chemometric software, and visualization tools essential for interpretation.</p></div><div><div><div><div><h4><strong>Heart-cutting 2D-LC (LC–LC)</strong></h4></div></div></div></div><div><p>Heart-cutting 2D-LC is a selective mode in which only specific fractions from the first dimension are reanalyzed in the second. This targeted strategy reduces analysis time, conserves solvent, and focuses resolution where it is absolutely required. It is widely applied for impurity profiling, regulatory compliance testing, and focused metabolomics.</p><p>Innovations like multiple heart-cutting (mLC–LC) and selective comprehensive LC×LC (sLC×LC) combine precision with broader analytical coverage. It offers flexible workflows that can adapt to both discovery and routine quality control needs.</p></div><div><div><div><div><h3><strong>When and Why Use Two-Dimensional Liquid Chromatography</strong></h3><p>Two-dimensional liquid chromatography (2D-LC) is mainly chosen when traditional one-dimensional <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/lc-an-indept-exploration"><strong>liquid chromatography</strong></a> (1D-LC) becomes inadequate to separate complicated mixtures. In most biological, pharmaceutical, and environmental samples, analytes tend to co-elute with similar physicochemical properties, which leads to difficult quantitation and identification.</p><p>The orthogonality of 2D-LC triumphantly overcomes these difficulties through high augmented peak capacity and resolution, providing an information depth that 1D-LC cannot possibly achieve. This strategy is especially useful in the study of intricate sample matrices. Plant extracts, biological fluids, foods, and environmental samples usually contain a vast number of compounds with comparable retention properties numbering in the thousands.</p><p>2D-LC enables the dissection of such chemical complexity to unmask minor constituents that might be biologically active or of environmental interest. It is now critical for untargeted metabolomics, lipidomics, environmental analysis, and natural product profiling, in which extensive coverage is preferred.</p><p>In regulated sectors like pharmaceuticals, two-dimensional chromatography is becoming commonly used to address stringent quality assurance and regulatory considerations. Where 1D-LC is unable to separate key impurities or degradation products, 2D-LC provides a robust platform for impurity profiling, stability testing, and lot-release assays. It reduces the effect of excipients and non-volatile buffer when using MS for impurity identification. The capacity to selectively target and define trace impurities delivers regulatory assurance and product safety.</p><p>Aside from resolution, 2D-LC provides greater sensitivity and quantitation. Simplifying matrix complexity reduces ion suppression in mass spectrometry processes, which enhances signal clarity and detection limits. This feature is essential for exploratory analysis, like finding new biomarkers or monitoring trace contaminants, where accuracy and reproducibility are of utmost importance.</p><p>With increasing analytical needs, 2D-LC is an ideal high-throughput, scalable, and future-proof solution for laboratories handling chemically complex and troublesome samples.</p></div></div></div></div><div><div><div><div><h3><strong>Common Applications of 2D-LC</strong></h3></div></div></div></div><div><p>Two-dimensional liquid chromatography (2D-LC) has evolved into a versatile analytical tool capable of resolving chemically complex samples that challenge conventional methods. By offering dramatically increased peak capacity and orthogonal selectivity, 2D-LC supports diverse industries ranging from pharmaceuticals to materials science. Below are some of its most impactful applications:</p></div><div><div><div><div><h4><strong>Biopharmaceuticals: mAbs, ADCs, and peptides</strong></h4></div></div></div></div><div><p>Biopharmaceutical development relies on 2D-LC to characterize monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), and therapeutic peptides. It provides high-resolution profiling of charge heterogeneity, glycosylation, and other post-translational modifications, streamlining development and regulatory submissions.</p></div><div><div><div><div><h4><strong>Proteomics and metabolomics</strong></h4></div></div></div></div><div><p>In proteomics, LC×LC enables deep peptide coverage post-digestion, advancing biomarker discovery. In metabolomics, it resolves thousands of metabolites in biological fluids, especially when integrated with high-resolution mass spectrometry.</p></div><div><div><div><div><h4><strong>Natural products and herbal extracts</strong></h4></div></div></div></div><div><p>2D-LC efficiently separates structurally similar phytochemicals, revealing trace bioactives crucial for drug discovery and nutraceuticals.</p></div><div><div><div><div><h4><strong>Food and beverage testing</strong></h4></div></div></div></div><div><p>2D-LC identifies flavor compounds, pesticides, mycotoxins, and additives, supporting quality control and safety standards in food sectors.</p></div><div><div><div><div><h4><strong>Petrochemical and environmental analysis</strong></h4></div></div></div></div><div><p>From petroleum fractions to persistent organic pollutants, LC×LC offers detailed compositional profiling for compliance and environmental protection.</p></div><div><div><div><div><h4><strong>Polymer characterization</strong></h4></div></div></div></div><div><p>2D-LC is used to examine polymer branching, blends, and molecular weight distributions.</p></div><div><div><div><div><h3><strong>Future Trends and Innovations in 2D-LC</strong></h3></div></div></div></div><div><p>Advancements in technology are rapidly transforming two-dimensional liquid chromatography into a more accessible, routine technique:</p><ul><li><strong>Miniaturization:</strong> Micro- and nano-LC systems reduce sample requirements and environmental impact while improving sensitivity and speed.</li><li><strong>Automated modulation:</strong> Innovations like active solvent modulation (ASM) and transfer modulation resolve solvent compatibility issues between dimensions, improving method robustness.</li><li><strong>Integration with mass spectrometry:</strong> LC×LC–MS is increasingly used in omics and pharmaceutical research for comprehensive structural elucidation.</li><li><strong>AI-driven data analysis:</strong> Artificial intelligence and machine learning algorithms simplify data interpretation and reduce analysis times.</li><li><strong>Green analytical chemistry:</strong> Solvent recycling and energy-efficient workflows are making 2D-LC more sustainable, aligning with global eco-conscious initiatives.</li><li><strong>3D chromatography:</strong> Research into three-dimensional chromatography hints at future developments, where even higher resolving power may become available.</li><li><strong>User-friendly software development:</strong> Efforts are underway to create more intuitive and compliant software solutions, making 2D-LC suitable for GMP laboratory environments.</li></ul></div>

2D-LC (Two-dimensional Liquid Chromatography): What, When and Why to Use it

Pipetting Made Easy – Part 1: Best Practice Guide

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 20th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / HPLC Symposium </strong>and<strong> Waters Corporation / ASMS </strong>and technical note by<strong> KNAUER!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Comprehensive characterization of oligonucleotides and related impurities using advanced LC/Q-TOF analytical workflows</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_oligonucleotide_lc_q_tof_mp_536_asms_2026_en_agilent_b896ffee63.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Therapeutic oligonucleotides are synthetic, short nucleic acid sequences designed to modulate gene expression by targeting RNA or DNA with high sequence specificity. However, their inherent structural heterogeneity makes impurity profiling essential, as during GMP manufacturing, impurities like phosphorothioate to phosphodiester, 5′ and 3′ truncations or extensions, depurination, and abasic species can directly impact both product safety and efficacy. Antisense oligonucleotides (ASO) like Nusinersen (7127.171 g/mol) are indicated for the treatment of spinal muscular atrophy (SMA). Their closely related variants coelute with the main peak, complicating their characterization in conventional one-dimensional LC/MS workflows. To address this challenge, a 2D LC/MS strategy was implemented using the Agilent 1290 Infinity III Bio LC system with multiple heart cutting, coupled to the 6545XT AdvanceBio LC/Q-TOF and MassHunter BioConfirm software. The first-dimension separation uses an ion pairing buffer-based method to achieve higher chromatographic peak resolution, while the second dimension provides MS compatible desalting, enabling enhanced mass spectrometric sensitivity and improved detection of low-level impurities.&nbsp;</p><h4><strong>Instrumentation</strong></h4><p>1290 Infinity III Bio 2D-LC &amp; <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6545XT"><strong>6545XT Q-TOF</strong></a> includes:&nbsp;</p><ul><li>1290 Infinity III Bio Flexible Pump (G7131A)&nbsp;</li><li>1290 Infinity III Bio High-Speed Pump (G7132A)&nbsp;</li><li>1290 Infinity III Bio Multisampler (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Variable Wavelength Detector (G7114B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B)&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=2D-LC"><strong>2D-LC System</strong></a> with MHC set up</li></ul><h4><strong>Conclusions</strong></h4><ul><li>A 2D-LC/MS workflow enabled effective separation and identification of coeluting impurities.&nbsp;</li><li>HiRes sampling with multi-injection improved resolution and throughput while preserving 1D separation for MScompatible desalting.&nbsp;</li><li>6545XT AdvanceBio LC/Q-TOF delivered accurate mass and MS/MS data for low-abundance impurity detection.&nbsp;</li><li>MassHunter BioConfirm software enabled automated sequence confirmation and impurity identification.&nbsp;</li><li>This integrated platform offers a robust solution for oligonucleotide characterization in therapeutic development.</li></ul><h3><strong>2. KNAUER: Smart Monitoring &amp; Optimization Solutions for SMB Processes: In-Line and Off-Line Tools for SMB Systems</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0048_knauer_in_line_and_off_line_tools_for_smb_systems_50a5f1c1cf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In liquid chromatography, the purification of target compounds is classically carried out using single column batch chromatography. More efficient approaches include semi – continuous systems with two to three columns and continuous multicolumn systems. The most widely used of the latter is the simulated moving bed (SMB) chromatography which enables the continuous separation of binary or pseudo-binary mixtures. SMB purification is used in various areas such as petrochemical, pharmaceutical or food industry for the production of fine chemicals in different quantities and purities ranging from gram to tons per day. SMB systems vary significantly in size from small lab scale systems operating columns with 8 mm inner diameter (ID) to industrial systems operating columns of onemeter ID or larger.</p><p>Ideally, an SMB process is developed in a smaller lab scale system to reduce solvent and sample consumption compared to development at production scale. Once the separation parameters are established, the SMB process allows for accurate up scaling from lab to production scale. During method development but also production, different tools can be used to monitor and control the process which can be distinguished between in-line and off-line measures. The most crucial parameter, in addition to measuring the pressure, is monitoring the flow rates of the pumps, outlets and/or zones accurately with additional flowmeters. Normally, SMB systems are not equipped with detectors, but UV or RI detectors can be integrated to monitor process stability.&nbsp;</p><p>A standard off-line tool is used to analyse samples taken for one switch or cycle at the raffinate and extract outlets. The resulting chromatograms are used to check, if the separation is working. Further, samples can be taken during the process allowing the depiction of concentration profiles in the different zones. These tools will be described in more detail in the following sections.</p><h4><strong>MATERIAL AND METHODS</strong></h4><ul><li><strong>AZURA Lab SMB system</strong>: SMB, biocompatible, 30 ml/min zone1, 4 ml/min feed, 10 – 130 bar&nbsp;</li><li><strong>AZURA Lab SMB system: </strong>SMB, stainless steel, 30 ml/min zone1, 4 ml/min feed, 10 – 130 bar&nbsp;</li><li><strong>AZURA Pilot SMB system, standard: </strong>SMB, stainless steel, 250 ml/min zone1, 40 ml/min feed, 2 – 100 bar&nbsp;</li><li><strong>AZURA Pilot SMB system, high flow:</strong> SMB, stainless steel, 400 ml/min zone1, 100 ml/min feed, 2 – 60 bar</li><li><strong>Refractive index detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2429"><strong>AZURA® RID2.1L HighFlow detector</strong></a> 100 ml/min&nbsp;</li><li><strong>UV detector: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2428"><strong>AZURA®UVD 2.1S detector</strong></a>, w/o flow cell</li><li><strong>UV detector: </strong>AZURA®UVD2.1S UV detector, fiber optics w/o flow cell</li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>SMB is a continuous separation process that can run for several hours or days without interruption. Therefore, it is important to monitor the process and to be able to adapt to changes during the run. Different tools are available that can be used for process monitoring and method development. These tools can be divided into two main categories: in-line and off-line tools. In-line tools monitor parameters such as pressure and flow rate directly during the run, whereas off-line tools take a sample, which is then analyzed by an HPLC system. The tools described can be added to the KNAUER SMB systems depending on the customer’s needs. The more parameters that are measured, the better the process can be monitored. This is especially helpful during SMB method development.</p><h3><strong>3. Shimadzu / ASMS: Accelerating drug screening in forensic hair analysis by applying high-speed polarity switching in HR LC-MS/MS</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_180_Accelerating_drug_screening_in_forensic_hair_analysis_by_applying_high_speed_polarity_switching_in_HR_LC_MSMS_168f8cc0b2.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In 2023, the Society of Hair Testing (SoHT) published updated consensus guidelines for the forensic analysis of drugs of abuse in hair, defining recommended targets across eight major drug classes: opiates, cocaine, amphetamines, cannabinoids, opioids, methadone, buprenorphine, and ketamine. Legal drivers, accepted use cases, and degree of prescription differ between US and Europe but in both jurisdictions, mass spectrometry is widely applied to support legally defensible conclusions. The challenge for any drug testing in hair samples, whether it is in forensic and safeguarding contexts, workplace testing or a judicial setting is the need to develop methods capable of detecting expanded search panels with the increased prevalence of new psychoactive substances (NPS) and non-medical prescription drug use. The approach applied in this work considered a LC/QTOF analysis using DDAMS/MS with rapid polarity switching with a novel data processing application, Insight Profiler. The software was designed to support a single click approach to automate data processing of complex data sets in non-targeted analysis from feature detection to compound identification.</p><h4><strong>Materials and Methods</strong></h4><h5><strong>Reversed phase LC Separation: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+X2"><strong>Nexera X2&nbsp;</strong></a></h5><ul><li>Shim-pack Velox Biphenyl (100 x 2.1 mm, 2.7 µm) column; column temp. 40 ºC, flow rate: 0.3 mL/min, 17 min total analysis time. Methanol:water gradient with 2 mM ammonium formate and 0.002% formic acid&nbsp;</li></ul><h5><strong>High resolution QTOF analysis: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-9050"><strong>LCMS-9050&nbsp;</strong></a></h5><ul><li>MS scan m/z 100-1000, 100 msec scan time. Polarity switching time 800 msec.&nbsp;</li><li>MS/MS DDA: 4 scans in positive ion mode (m/z 40-1000, 33 msec), 2 scans in negative ion mode (m/z 40-1050, 33 msec) CE 5-55V (1.998 sec total cycle time).</li></ul><h5><strong>Data processing</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Insight+Profiler"><strong>Insight Profiler</strong></a>, feature detection threshold set to low, compound identification search lists included NIST, HighResNPS, Shimadzu Forensic Toxicology database.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>The targeted DDA-MS/MS polarity switching approach successfully identified a significant number of unknown compounds in the samples not routinely targeted by triple quadrupole analysis.&nbsp;</li><li>The method using DDA-MS/MS was easily configured to support an expanded DoA target panel to meet the needs of forensic toxicology screening.&nbsp;</li><li>Non-targeted analysis using Insight Profiler accelerated data analysis by aligning identified compounds from the same hair donor showing direction of change for pharmaceutical and illicit compounds detected.</li></ul><h3><strong>4. Thermo Fisher Scientific / HPLC Symposium: Enhancing semi-volatile analyte detection in HPLC-CAD using temperature coupling mode for optimized evaporation control</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004766_hplc_vanquish_cad_fatty_acid_quantification_po004766_na_en_4a0868c2cb.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Polysorbates are widely used in biopharmaceutical formulations but can degrade or contain FAs impurities that must be accurately measured to ensure product quality and safety. Charged Aerosol Detection is commonly used for FA analysis, though its performance depends strongly on EvapT. Higher EvapT improves S/N ratios but can reduce sensitivity for semi-volatile compounds like FAs, while lower EvapT has the opposite effect. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2176"><strong>Thermo Scientific Vanquish Charged Aerosol Detector P</strong></a> series introduces a Temperature Coupling Mode that links EvapT with the charging detection module (CDM) temperature, overcoming limitations of earlier systems with fixed CDM temperature of 40 ºC. This coupling improves response and measurement reliability for semi-volatile species, enabling better optimization of FA analysis<sup>1</sup>.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>Advanced temperature control in CAD improved method sensitivity up to ~47% for semi-volatile fatty acids.&nbsp;</li><li>The best overall S/N was achieved at 25.0 °C EvapT with Temperature Coupling Mode enabled.&nbsp;</li><li>The optimized CAD approach supports more sensitive FA impurities and degradants quantification in pharmaceutical and biopharmaceutical formulations.</li></ul><h3><strong>5. Waters Corporation / ASMS: High mass and spatial resolution MS imaging of soft fruit sample cross-sections using desorption electrospray ionization (DESI)</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009492_282619e388.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>This poster demonstrates the use of <strong>desorption electrospray ionization mass spectrometry imaging (DESI MSI)</strong> for visualizing the spatial distribution of metabolites in high-water-content soft fruits, specifically strawberries and raspberries. The authors developed a simple direct-transfer workflow to overcome the challenges associated with imaging delicate fruit tissues and compared three transfer substrates: plain glass microscope slides, nitrocellulose membranes, and Hamamatsu Poropare plates. The goal was to determine which surface best preserves metabolite localization while enabling high-quality mass spectrometry imaging.</p><p>Fresh fruit cross-sections were transferred to each substrate by direct contact for 10 seconds before analysis using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=DESI+XS"><strong>Waters DESI XS ion source</strong></a> coupled to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2433"><strong>Waters Xevo™ MRT P10 high-resolution mass spectrometer</strong> </a>operating in negative electrospray ionization mode. MS imaging was performed with a <strong>50 µm pixel size</strong>, using a <strong>95:5 methanol/water</strong> spray solvent, while sucrose (m/z 341.1089) served as the lock mass for accurate mass calibration. High mass resolution enabled tentative assignment of metabolites such as citric acid, asparagine, malic acid, glutamic acid, palmitic acid, stearic acid, and sucrose.</p><p>The comparison of transfer substrates revealed significant differences in metabolite localization and image quality. Glass slides produced the strongest metabolite signals but also exhibited considerable metabolite delocalization during sample transfer, reducing spatial accuracy. Nitrocellulose membranes transferred a broader range of metabolites and provided complementary chemical information, although they also showed greater analyte spreading and suffered physical damage after a single DESI acquisition. In contrast, <strong>Poropare plates</strong> minimized metabolite delocalization and preserved fine structural details within the fruit cross-sections, despite producing somewhat lower signal intensities than glass slides.</p><p>The study concludes that direct transfer combined with DESI MSI is an effective approach for imaging soft fruit tissues that are otherwise difficult to analyze because of their high water content. Among the evaluated substrates, <strong>Hamamatsu Poropare plates</strong> provided the best balance between spatial resolution and preservation of metabolite localization, making them particularly suitable for high-resolution metabolomic imaging, while high mass accuracy facilitated confident tentative metabolite identification.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 30, 2026

Article

Product

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Technology Networks

SelectScience

SCIEX

Waters Corporation

<p><strong>PFAS &amp; Our Planet is a webinar series on PFAS testing in environmental samples, food and food contact materials, textiles and other materials. Alongside the laboratory and regulatory lens, the series addresses the broader planetary impact of PFAS, including their persistence and movement through water, soil, air, food systems, and materials. It brings together what laboratories and industry need to understand now: where PFAS rules are heading, which sample types are under increasing focus, what needs to be measured, and how those needs translate into method selection, workflow setup, and result reporting.</strong></p><h4><strong>Webinar Series Details</strong></h4><ul><li>One registration to attend the full three-part series</li><li>Questions for experts in advance and/or live Q&amp;A</li><li>Access to recorded sessions provided</li></ul><h4><strong>Who Should Attend:</strong>&nbsp;</h4><p>Environmental and water testing laboratories, food testing laboratories, food contact material and packaging laboratories, textile and materials testing laboratories, contract testing laboratories, public sector and regulatory laboratories, manufacturers of food, packaging, textiles, and consumer goods, quality, product safety, regulatory affairs, and sustainability functions, and research laboratories working on PFAS analysis.</p><h4><strong>Recommended for:&nbsp;</strong></h4><p>Analytical chemists, laboratory managers, QA/QC managers, regulatory affairs managers, food safety and product safety managers, environmental scientists, packaging and food contact materials specialists, textile and materials testing leads, sustainability and product stewardship managers, validation and method development scientists, and technical leaders in contract, public sector, and research laboratories.</p><h4><strong>Regulatory context covered:&nbsp;</strong></h4><p>EU and U.S. PFAS developments, food contact and packaging measures, environmental monitoring frameworks, OEKO-TEX requirements for textiles and materials, Singapore’s food and water surveillance activity, India’s draft PFAS-related packaging amendment, and emerging movement in parts of Southeast Asia like Thailand, Malaysia, Vietnam, Indonesia, Bangladesh and Philippines.</p><h3><strong>Textiles and Materials</strong></h3><p><strong>Textiles and treated materials are facing stronger PFAS scrutiny as product restrictions widen and supply chains are asked to verify material content more clearly. This session will cover the main external reference points shaping that shift, including the EU REACH PFAS restriction process and OEKO-TEX requirements, which have become highly relevant for textile and material suppliers serving global brands.</strong></p><p>It will then step into the laboratory workflow for textiles and related materials, covering extraction and cleanup, targeted LC/MS/MS for non-volatile PFAS, selected GC/MS/MS approaches for volatile PFAS where relevant, contamination control, and reporting formats suited to product verification. Regional context will be framed around export-market pull, buyer specifications, and certification-driven testing, which are often the main drivers for suppliers in South and Southeast Asia.</p><blockquote><p><strong>Presenter: </strong>David Saladin (Ecology Lab Team Leader, TESTEX AG, Swiss Textile Testing Institute)</p><p><strong>Presenter:&nbsp;</strong>Aimei Zou, Ph.D. (Application Scientist, Agilent Technologies, Singapore)</p></blockquote>

Agilent Technologies: PFAS & Our Planet: Textiles and Materials

PFAS & Our Planet: Textiles and Materials

PFAS in textiles webinar: regulations, LC-MS/MS and GC-MS/MS workflows, contamination control, and compliance for global supply chains.

<p><strong>In this SelectScience® TechTalk, Dr. Stefan Christian Gaugler, Professor of Applied Analytics at the Institute for Chemistry and Bioanalytics, University of Applied Sciences Northwestern Switzerland (FHNW), will provide an up-to-date overview of per- and polyfluoroalkyl substances (PFAS), focusing on their environmental impact, regulatory landscape, and associated analytical challenges. Highlighting the growing concern surrounding PFAS contamination across Europe, alongside the complexity arising from their chemical diversity and persistence.</strong></p><p>The session will explore current analytical workflows and sample preparation techniques, including the use of a novel PFAS-selective polymer for micro solid-phase extraction (µSPE), supporting efficient and automated analysis across diverse sample matrices. Attendees will gain insight into approaches for detecting both ultra-short and long-chain PFAS, as well as practical strategies to improve sensitivity, selectivity, and sustainability in PFAS analysis.</p><h4><strong>What will this webinar cover?</strong></h4><ul><li>The environmental relevance and regulatory developments surrounding PFAS in Europe.</li><li>The analytical challenges associated with PFAS diversity, including ultra-short and long-chain compounds.</li><li>Modern analytical workflows for PFAS detection using LC-MS/MS and GC-MS/MS techniques.</li><li>The advantages of novel PFAS-selective polymer-based µSPE methods for efficient and automated sample preparation.</li></ul><blockquote><p><strong>Speaker: </strong>Prof. Dr. Stefan Christian Gaugler (Professor of Applied Analytics at the Institute for Chemistry and Bioanalytics, University of Applied Sciences Northwestern Switzerland (FHNW))</p><p>Prof. Dr. Stefan Christian Gaugler is a researcher and lecturer at FHNW with a background in life sciences and analytical chemistry, driven by a long-standing interest in translating complex biomarker diagnostics into accessible solutions. Over the past 14 years, his work has focused on microsampling technologies, particularly dried blood spot (DBS) analysis.</p><p>He currently leads the micro lab at FHNW, where the team operates one of the world’s most technologically advanced dried blood spot laboratories and develops advanced methods for trace analysis of emerging contaminants. Alongside his research, he teaches advanced analytical techniques and laboratory automation, with a strong focus on bridging academia and real-world application.</p><p>His current work, including this presentation, focuses on advancing PFAS analysis to better understand and address their impact on human health and the environment.</p></blockquote><blockquote><p><strong>Moderator: </strong>Cameron Smith-Craig (Pharma and Applied Sciences Editor, SelectScience)</p></blockquote>

SelectScience: Advancing PFAS analytics across complex matrices

Advancing PFAS analytics across complex matrices

Discover automated µSPE solutions for PFAS analysis. Learn strategies from FHNW experts to overcome matrix challenges for ultra-short & long chains.

Advancing PFAS Analytics Across Complex Matrices via µSPE

<p><strong>Choosing the right HPLC column can be challenging given the wide range of stationary phases, particle technologies, and column dimensions available today. Whether you’re aiming to accelerate your separations, extend the performance of an older LC system, or unlock the full capability of a modern Agilent UHPLC platform, selecting the optimal column is key to achieving reliable, reproducible results.</strong></p><p>In this webinar, we’ll explore how Agilent’s column technologies—from traditional fully porous ZORBAX materials to advanced superficially porous InfinityLab Poroshell particles—can be strategically matched to your instrument and your analytical goals. We’ll discuss how particle size and column dimensions influence efficiency and back pressure, helping you balance resolution, speed, or instrument compatibility.</p><p>Beyond standard C18 phases, we’ll highlight a range of Agilent bonded phase chemistries designed to enhance selectivity and help you make informed choices for your next HPLC or UHPLC separation.</p><blockquote><p><strong>Speaker:</strong> Mark Powell&nbsp;</p><p>Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.</p></blockquote>

Agilent Technologies: Becoming A Better Chromatographer Webinar Series

Column Chemistry Compass: Navigating the Agilent HPLC and UHPLC Column Landscape

Master Agilent HPLC/UHPLC column selection. Compare fully porous ZORBAX and superficially porous Poroshell particles to optimize method development

Navigating the Agilent HPLC and UHPLC Column Landscape

<p><strong>This webinar introduces the fundamentals of monoclonal antibody (mAb) Critical Quality Attributes (CQAs) and the LC workflows commonly used to assess them. We’ll begin with an overview of mAb heterogeneity and the major CQA categories, including size, charge, hydrophobic and structural variants, plus common post-translational modifications.</strong><br><br>The session will then connect these CQAs to the chromatographic techniques most commonly used for characterization, including size exclusion chromatography (SEC), ion exchange chromatography (IEX), and reversed-phase LC (RPLC). Attendees will gain a practical understanding of what each technique measures, how the methods complement one another, and why they are important in biopharmaceutical analysis.</p><h4><strong>Key Learning Points</strong></h4><ul><li>Describe monoclonal antibodies (mAbs), including their basic structure, production, and role as therapeutic medicines.</li><li>Define Critical Quality Attributes (CQAs) and their relationship to product quality, safety, and performance.</li><li>Introduce key LC techniques used in mAb characterization, including SEC, IEX, and RP-LC.</li><li>Explain what each technique measures, how they complement each other in mAb workflows, and how to select appropriate methods for different CQAs.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any chromatography users working with or interested in monoclonal antibody (mAb) analysis and characterization by LC. &nbsp;</li></ul><h4><strong>Viewing Options</strong></h4><h5><strong>Session 1</strong></h5><p><strong>Tuesday, July 28, 2026</strong></p><ul><li>Los Angeles: 10:00 AM PT | New York: 1:00 PM EST</li><li>Mexico City: 12:00 PM CDT</li></ul><h5><strong>Session 2</strong></h5><p><strong>Wednesday, July 29, 2026</strong></p><ul><li>London: 9:00 AM BST | Berlin: 10:00 AM CEST&nbsp;</li><li>Mumbai: 1:30 PM IST</li></ul><blockquote><p><strong>Presenter: </strong>Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)</p><p>Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.&nbsp;</p></blockquote>

Phenomenex: Fundamentals First Bio Series - A Beginner’s Roadmap to mAb CQAs and LC Workflows

Fundamentals First Bio Series: A Beginner’s Roadmap to mAb CQAs and LC Workflows

Introduction to monoclonal antibody (mAb) CQAs. Explore how size exclusion, ion exchange, and reversed-phase LC workflows analyze bioheterogeneity.

Beginner’s Roadmap to mAb CQAs and LC Workflows

<h4><strong>Optimize Upstream Control with Data‑Driven Cell Media Analysis &amp; Titer Assessment</strong></h4><p><strong>This session explores how advanced analytics enable tighter control of upstream bioprocesses. We begin with a walkthrough of recent work in cell culture media analysis, highlighting vitamin profiling as a tool to better understand nutrient utilization and process performance.</strong></p><p>The story continues with focus on optimizing titer measurement using analytical Protein A, demonstrating benefits such as higher sensitivity for measurement earlier in cell cultures, faster results, improved data quality, and reduced workflow complexity to support more informed, timely upstream decision‑making.</p><h5><strong>Key takeaways:</strong></h5><ul><li>Understand how advanced cell media analysis, including vitamin profiling, enhances understanding of nutrient utilization and upstream process performance.</li><li>Evaluate how analytical Protein A titer measurements enable earlier, faster, and more sensitive assessment of product formation during cell culture.</li><li>Apply data‑driven analytical insights to improve upstream process control, data quality, and decision‑making efficiency.</li></ul><h5><strong>This Webinar is designed for:</strong></h5><ul><li><strong>Upstream Process Development &amp; Engineering Teams</strong> – Scientists and engineers focused on media optimization, nutrient utilization, and improved process control.</li><li><strong>MSAT &amp; Process Characterization Professionals </strong>– Teams seeking deeper process understanding and data‑driven decision making across development and manufacturing.</li><li><strong>Analytical Development Scientists</strong> – Experts responsible for cell media and titer analysis who want faster, more sensitive, and streamlined analytical workflows.</li></ul><blockquote><p><strong>Speaker:</strong> Mark Wrona (Consulting Scientist, Biologics Business Unit, Waters Corporation)</p><p>Mark Wrona manages specialized teams of scientists developing analytical applications to support drug development and PAT applications.</p><p>He specializes in biologics and life sciences applications.He has 15 years with Waters and prior experience as a research chemist at a large pharma.</p></blockquote><blockquote><p><strong>Speaker:</strong> Fang Lu (Senior Upstream Process Development Scientist, Waters Corporation)</p><p>Dr. Fang Lu is a Senior Upstream Process Development Scientist at Immerse Delaware, Waters Corporation.</p><p>She has expertise in viral vector upstream process development, gene therapy manufacturing, and monoclonal antibody upstream development, with a focus on applying analytical tools to enhance process understanding and optimization.</p><p>Prior to joining Waters, Fang spent four years in the cell and gene therapy CDMO industry supporting process development for complex biologics and advanced therapy applications.</p></blockquote>

Data‑Driven Upstream Control: Optimizing Cell Media Analysis and Titer Assessment

Enhance upstream control with data-driven analytics. Discover how vitamin profiling in media and high-sensitivity Protein A titer assays speed up PD.

Optimizing Cell Media Analysis and Titer Assessment

<div><p><strong>As adeno-associated virus (AAV) therapies advance toward larger-scale manufacturing, achieving consistent full capsid enrichment remains a critical challenge. Variability between serotypes and purification conditions can affect product quality, recovery, and process scalability.</strong></p><p>In this webinar, Chiu Lau-Barre will share practical strategies for developing robust anion exchange (AEX) polishing workflows, drawing on real-world AAV2 and AAV9 examples.</p><p>Attendees will gain insight into approaches for improving purification performance, streamlining process development, and supporting efficient scale-up. The session will also highlight how modern screening tools can help accelerate decision-making and reduce development timelines.</p><h5><strong>Attend this webinar to:</strong></h5><ul><li>Understand key variables that influence AEX polishing performance of full/empty capsid separation for AAVs</li><li>Explore AAV2 and AAV9 case studies demonstrating how AEX polishing methods can be optimized across serotypes</li><li>Learn how high-throughput AEX screening can accelerate process development and support scalable workflow implementation</li><li>Identify process development strategies that can help reduce optimization timelines and support scale-up readiness</li></ul><blockquote><p><strong>Presenter:</strong> Chiu Lau-Barre (Staff Scientist, Field Applications at Thermo Fisher Scientific)</p><p>Chiu Lau-Barre is a staff scientist at Thermo Fisher Scientific, where she supports customers in purification process development and troubleshooting across a range of biomolecules, including antibodies and adeno-associated viral (AAV) vectors. With more than 15 years of biopharmaceutical industry experience, she has held scientific leadership roles at Pfizer and Eli Lilly, contributing to antibody and protein therapeutic development from early-stage screening through process development. Her expertise spans purification strategy, developability assessment and scalable bioprocess workflows. Chiu holds a bachelor's degree in Biochemistry from the University of California, Santa Barbara.</p></blockquote></div>

Technology Networks: Polishing Strategies for Full Adeno-Associated Capsid Enrichment

Polishing Strategies for Full Adeno-Associated Capsid Enrichment

In this webinar, strategies for developing robust anion exchange (AEX) polishing workflows, drawing on real-world AAV2 and AAV9 examples.

Strategies for Full Adeno-Associated Capsid Enrichment

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Today's analytical laboratories face increasing pressure to deliver faster turnaround times, higher sensitivity, and greater throughput while optimizing resources, controlling operating costs, and meeting sustainability objectives. As analytical demands continue to grow, laboratories need innovative technologies that enhance productivity without compromising data quality or environmental responsibility.</strong></p><p>Join us to explore how the SCIEX Novus V55 System is ushering in a new era of sustainable high-performance mass spectrometry. Designed to maximize laboratory efficiency, the Novus V55 System enables faster quantitative workflows, increased sample throughput, and outstanding analytical performance, while reducing energy consumption and cooling requirements. Discover how you can achieve more with less—boosting productivity, lowering operational costs, and advancing your sustainability goals without sacrificing performance.</p><h4><strong>In this webinar, you will learn:</strong></h4><ul><li>Learn how to scale quantitative workflows to 1,000 MRMs/sec—boosting throughput without compromising data quality&nbsp;</li><li>Discover how to increase sample throughput by ~33% while maintaining sensitivity and reproducibility.</li><li>See how to reduce lab operating costs with up to 40% lower energy and cooling requirements</li></ul><blockquote><p><strong>Presenter: </strong>Aman Sharma (Sales Development Manager)</p></blockquote></div></div></div>

SCIEX: More with less - The new era of sustainable high-performance mass spectrometry

More with less: The new era of sustainable high-performance mass spectrometry

Discover the SCIEX novus V55 system. Learn how aeMRM technology and a 35% smaller footprint deliver sustainable, high-throughput LC-MS/MS

SCIEX novus V55: Sustainable High-Performance MS

<div><h4><strong>Unlock the Power of Full-Spectrum Flow Cytometry</strong></h4><p><strong>A two-part masterclass designed to help you understand, design, and apply high-dimensional flow cytometry workflows across immunology, oncology, and vaccine research.</strong></p><h5><strong>Session 2: Designing and Analysis of Spectral Flow Experiments</strong></h5><ul><li><strong>Key Advantages of Spectral Technology. </strong>Learn how spectral systems enable higher multiplexing, improved resolution, and enhanced flexibility in panel design for complex experiments.</li><li><strong>Workflow of Spectral Flow Cytometry. </strong>Step through the complete experimental journey—from panel design and sample preparation to acquisition and interpretation of high-dimensional data.</li><li><strong>Unmixing and Data Analysis Strategies. </strong>Gain insights into spectral unmixing principles and advanced analytical approaches that transform raw data into meaningful biological interpretation.</li></ul><h5><strong>Why Attend?</strong></h5><ul><li>Move beyond conventional flow limitations</li><li>Design high-dimensional experiments confidently</li><li>Learn real-world applications</li><li>Interact with flow cytometry experts</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Hemant Agrawal (Director, Flowcytometry Solutions Pvt. Ltd., India)</p><p>Flow cytometry educationalist and global trainer with 15+ years of experience. Conducted 80+ onsite and 40+ online workshops worldwide. An active member of ISAC (International Society for Advancement of Cytometry) task forces, Dr. Agrawal is widely recognized for advancing cytometry education globally and has been an invited faculty at prestigious Indo-US Cytometry Workshops.</p></blockquote><blockquote><p><strong>Presenter:</strong> Dr. Saket Siddharth (Field Application Scientist – Flowcytometry, Agilent Technologies)</p><p>Saket Siddharth is a Flow Cytometry and Cell Analysis specialist with over nine years of experience spanning clinical cytometry, spectral cell sorting, and advanced multicolor panel design. He holds a Ph.D. from the Helmholtz Centre for Infection Research, Brunswick, Germany, and has developed strong expertise across antibody development, high-throughput screening, and assay validation for translational and research applications.</p></blockquote></div>

Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 2

Spectral Flow Cytometry Masterclass - Session 2

Learn high-dimensional spectral flow cytometry workflows with Cytek. Master panel design, sample prep, and advanced spectral unmixing strategies

Spectral Flow Cytometry Masterclass

<p><strong>Join us for an insightful webinar exploring how integrated analytical workflows are transforming applications of mass spectrometry technologies. Discover how biomedical researchers are leveraging the throughput of a seamless workflow of automated sample preparation, complex quantitative assays, and streamlined data processing for large-scale biomedical research projects and environmental hazard monitoring applications.</strong></p><p>Through expert talks and real-world collaboration with Agilent Technologies, this session will showcase the power of the state-of-the-art mass spectrometry technology.</p><h4><strong>Why You shouldn’t Miss This:</strong></h4><ul><li>Learn how integrated analysis workflows enable large-scale studies</li><li>Gain insights into the role of lipids as accurate biomarkers for cardiovascular disease risk stratification</li><li>Understand the importance of human biomonitoring of environmental chemical exposure and its implications</li><li>Discover how industry-academic collaboration drives innovation</li></ul><h4><strong>Agenda:</strong></h4><ol><li><strong>Analytical Workflows in Omics Space. </strong>- Dr. Ravindra Gudihal, GSDC, Agilent Technologies</li><li><strong>Integrative cardiometabolic disease research with metabolites and genes.</strong> - Assoc. Prof. Hyungwon Choi, SLING, NUS</li><li><strong>Exposomic monitoring and automation </strong>- challenges and solutions in driving throughput for large cohort analysis and in longitudinal studies. - Dr. Amaury Cazenave-Gassiot, SLING, NUS</li><li><strong>Collaboration with Agilent. </strong>- Dr. Ravindra Gudihal, GSDC, Agilent Technologies</li></ol><blockquote><p><strong>Presenter:</strong> Prof. Hyungwon Choi (Director, SLING, National University of Singapore)</p><p>Dr. Choi was trained as computational biologist with doctoral and post-doctoral traininginUniversityof Michigan Ann Arbor, USA. He joinedNUS faculty in 2011 and is currently associate professor in the Department of Medicine. He serves as the chair of Cardiovascular-MetabolicDiseaseTranslational ResearchProgramme in Yong Loo Lin School of Medicine and the director of Singapore Lipidomics Incubator in Life Sciences Institute at the university. In his early careerhis main research covered computational proteomics, protein-centric analysis of genomic and transcriptomic data in large-scale clinical studies, and network-driven integration of multi-omics data. His most recent research expands into bioinformatics workflow development for raw mass spectrometry data processing in targeted and untargeted metabolomics and lipidomics, incorporating deep learning algorithms for cheminformatics. During the fifteen years in NUS, his team has developed innovative data analytic approaches to parse signals from mass spectrometry-based proteomics and metabolomics data, describe cellular dynamics in terms of protein abundance, modifications, and interaction, and derive accurate measurements of small molecules.Leveragingon these diverse capabilities, his team has led data generation and analysis in multiple clinical biomarker studies in type II diabetes, diabetic complications, cardiovascular disease, and cognitive decline and other aging-related diseases conducted in Singapore.</p></blockquote><blockquote><p><strong>Presenter:</strong> Dr. Amaury Cazenave-Gassiot (Research Assistant Professor, SLING, National University of Singapore)</p><p>Dr. Amaury Cazenave-Gassiotis a Research Assistant Professor in the Singapore Lipidomics Incubator (SLING) and CVMD Translation Research Programme at the National University of Singapore (NUS).</p><p>Since joining NUS in 2009, Dr. Cazenave-Gassiot has specialised in mass spectrometry-based analysis, gaining experience in lipidomics and lipid biochemistry.</p><p>He has contributed his analytical expertise to a broad range of collaborative research projects spanning animal models and human pre-clinical samples across diverse lipid classes. His research focuses on understanding lipids and lipid metabolism in the context of health, disease, and ageing, with applications extending from cardiovascular disease to emerging areas such as cancer, depression, or brain diseases.</p><p>As a member of the international lipidomics community, he contributes to the development of effective, reliable analytical methods and the harmonization of lipidomic practices across the field. As co-PI of the R&amp;D node within the NUS–Agilent Hub for Translation and Capture, he leads efforts to build robust, high-throughput mass spectrometric assays and to facilitate the adoption of omics technologies. This work encompasses the validation and application of assays to pre-clinical cohorts, bridging fundamental research and translational science. More recently, he has expanded his research scope towards exposomics, leveraging his mass spectrometry expertise, he is developing analytical approaches to detect and quantify these persistent environmental contaminants (such as per- and polyfluoroalkyl substances, PFAS) in human cohorts.</p></blockquote><blockquote><p><strong>Presenter: </strong>Dr. Ravindra Gudihal (Application Scientist, Agilent Technologies, Inc)</p><p>Ravindra Gudihal is a Senior Solution Scientist at Agilent Technologies Singapore, specializing in advanced analytical workflows for biopharma. He holds a Ph.D. in Molecular Biophysics from the Indian Institute of Science and has published over 20 peer-reviewed articles. Ravindra is passionate about bridging science and innovation to transform how we understand and develop life-saving therapies.</p></blockquote>

Agilent Technologies: Empowering Omics Research Through Integrated Workflows and Strategic Collaboration

Empowering Omics Research Through Integrated Workflows and Strategic Collaboration

Discover Agilent mass spectrometry workflows for large-scale omics. Learn how lipid biomarkers improve cardiovascular disease risk stratification

Empowering Omics Workflows and Strategic Collaboration

<p><strong>As materials become increasingly sophisticated, understanding their optical properties is essential for driving innovation, improving product performance, and ensuring quality. Whether developing anti-reflective coatings, optimising solar cell efficiency, designing specialty glass, or researching next-generation materials, accurate optical characterisation is a critical part of the workflow.</strong></p><p>Join us for this webinar to discover how the Agilent UV-Vis-NIR spectrophotometer enables researchers and industry professionals to measure and characterise a broad range of optical properties across diverse sample types and applications.</p><p>The webinar will showcase measurement techniques for transmission, reflectance, diffuse reflectance, and angular measurements, highlighting how the Agilent UV-Vis-NIR platform delivers reliable and reproducible data for both research and industrial applications.</p><h4><strong>Why Attend?</strong></h4><p><strong>In this session, you will learn:</strong></p><ul><li>How the Agilent UV-Vis-NIR can be used to characterise thin films, coatings, solar materials, and glass.</li><li>Techniques for measuring transmission, reflectance, and diffuse reflectance with confidence.</li><li>Methods for determining film thickness and evaluating optical performance.</li><li>How specialised accessories expand measurement capabilities for challenging samples.</li><li>Best practices for obtaining accurate, reproducible results across a variety of applications.</li><li>Real-world examples demonstrating how optical measurements can accelerate research and product development.</li></ul><h4><strong>Who Should Attend?</strong></h4><p><strong>This webinar is designed for:</strong></p><ul><li><strong>Academic Researchers and Students</strong><ul><li>Researchers in materials science, physics, chemistry, and engineering.</li><li>Scientists investigating thin films, nanomaterials, optical materials, and photonic devices.</li><li>Graduate students and postdoctoral researchers seeking advanced optical characterisation techniques.</li></ul></li><li><strong>Solar and Photovoltaics</strong><ul><li>Scientists and engineers developing solar cells and photovoltaic materials.</li><li>Researchers evaluating anti-reflective coatings and light management strategies.</li><li>Teams working to improve solar cell efficiency through optical optimisation.</li></ul></li><li><strong>Glass and Coatings Industries</strong><ul><li>Manufacturers of architectural, automotive, and specialty glass.</li><li>Researchers developing low-emissivity and anti-reflective coatings.</li><li>Quality control and R&amp;D professionals responsible for optical performance testing.</li></ul></li><li><strong>Advanced Materials and Industrial R&amp;D</strong><ul><li>Thin film and semiconductor researchers.</li><li>Optical coating manufacturers.</li><li>Scientists working with displays, sensors, filters, and advanced functional materials.</li><li>Analytical laboratories requiring precise optical characterisation.</li></ul></li></ul><h5><strong>Featured Applications</strong></h5><ul><li>Thin film and multilayer coating analysis</li><li>Anti-reflective and optical coating characterisation</li><li>Solar cell and photovoltaic material evaluation</li><li>Optical filter transmission and blocking measurements</li><li>Architectural and specialty glass analysis</li><li>Reflectance measurements at varying angles of incidence</li><li>Film thickness determination</li><li>Diffuse reflectance measurements of scattering materials</li><li>Optical characterisation of emerging materials in academia and industry</li></ul><p>Discover how the Agilent UV-Vis-NIR platform combines exceptional optical performance, flexible sampling accessories, and intuitive workflows to provide the insights needed to advance research, optimise products, and solve challenging analytical problems.</p><blockquote><p><strong>Presenter: </strong>Mr. Rajwar Chandra (Product Specialist - Spectroscopy, Agilent Technologies)</p><p>Chandra Rajwar is a Product &amp; Market Specialist with over 20 years of experience in Molecular Spectroscopy products and analytical instrumentation. He specializes in UV-Vis, FTIR, Raman, and related spectroscopy solutions, supporting customers with application development, workflow optimization, and analytical excellence across pharmaceutical, chemical, environmental, and research industries.</p></blockquote><blockquote><p><strong>Presenter: </strong>Ms. Vijaya Jyothi (Product Specialist - Spectroscopy, Agilent Technologies India Pvt. Ltd.)</p><p>Vijaya Jyothi is a seasoned product specialist at Agilent Technologies. She manages the spectroscopy division, having extensive expertise in spectroscopy applications across diverse industries, offering deep insights and solutions tailored to varied analytical needs.</p></blockquote>

Agilent Technologies: Characterising Advanced Materials with the Agilent UV-Vis-NIR - From Thin Films and Solar Cells to Glass and Optical Coatings

Characterising Advanced Materials with the Agilent UV-Vis-NIR: From Thin Films and Solar Cells to Glass and Optical Coatings

Characterize thin films, solar cells, and coatings with Agilent UV-Vis-NIR. Master reflectance, transmission, and angular measurement workflows

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