<p><strong>The team of Professor&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en"><strong>Michal Hocek</strong></a><strong> at IOCB Prague explores the limits of the structure and function of DNA and successfully pushes forward known boundaries. An article presenting the results of their latest research has just been published in the major scientific journal </strong><i><strong>Nucleic Acids Research</strong></i><strong>. They prove that even heavily modified double helices of DNA are stable enough to be used for special applications. This discovery might expand the possibilities of utilizing modified DNA, for example, in medicine.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Tomas_Bellon_Scientists_from_IOCB_Prague_are_pushing_the_limits_of_DNA_b5c448f9a9.jpg" alt="ÚOCHB: Scientists from IOCB Prague are pushing the limits of DNA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Tomas_Bellon_Scientists_from_IOCB_Prague_are_pushing_the_limits_of_DNA_b5c448f9a9.jpg 245w," sizes="100vw" width="1910" height="1000"></p><p>DNA consists of two long negatively charged chains that hold together even though they repel each other. This is made possible by what is known as stacking interactions and base pairing. Michal Hocek and his colleagues advanced the known limits when they modified DNA by adding an extra negative charge to each letter of the genetic code. This doubled the repulsive forces, yet the DNA double helix remained stable. They also found that such modified DNA not only holds together but that it can also be synthesized or replicated and sequenced using the enzyme DNA polymerase.</p><p><i>"Think of DNA as a scaffold on which you can attach various chemical compounds with different functions. These are small molecules, such as side chains of amino acids, which occur naturally only in peptides and proteins. In current medicine, we are able to use these molecules only to a relatively limited extent. The reason is that they are markedly unstable and get decomposed rapidly when inside an organism. One solution to this would be a stable skeleton to which they could be firmly attached. And DNA could be just such a base structure in the future,"</i> says Prof. Michal Hocek.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Tomas_Bellon_Scientists_from_IOCB_Prague_are_pushing_the_limits_of_DNA_Prof_Michal_Hocek_Distinguished_Chair_and_head_of_Bioorganic_and_Medicinal_Chemistry_of_Nucleic_Acids_0a8c924ac4.jpg" alt="ÚOCHB / Tomáš Belloň: Prof. Michal Hocek, Distinguished Chair and head of Bioorganic and Medicinal Chemistry of Nucleic Acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Tomas_Bellon_Scientists_from_IOCB_Prague_are_pushing_the_limits_of_DNA_Prof_Michal_Hocek_Distinguished_Chair_and_head_of_Bioorganic_and_Medicinal_Chemistry_of_Nucleic_Acids_0a8c924ac4.jpg 234w," sizes="100vw" width="1500" height="1001"></p><p>The aim of this research carried out at IOCB Prague is to create DNA molecules that will mimic other chemical compounds. This would enable us to leverage the medicinal potential of certain biomolecules that are difficult to retain in the body. Examples of such biomolecules are the already mentioned peptides or proteins.</p><p>The Hocek group has already made significant progress, although research in this direction is at its beginning worldwide. The team has, for example, succeeded at developing a novel modified aptamer, which is a short DNA sequence that can bind to a specific target molecule, most frequently a protein. Aptamers have similar properties to antibodies, but they are much more stable. For this reason, aptamers can potentially replace antibodies employed in medicine. However, the number of approved therapeutic aptamers globally can still be counted on the fingers of one hand.</p><p>The current research exploring the limits of DNA is part of a larger project led by Michal Hocek, for which he has secured a prestigious EXPRO grant from the Czech Science Foundation. This is the second time its results have drawn interest from the journal <i>Nucleic Acids Research</i>. Previously, scientists from IOCB Prague published a method for the enzymatic synthesis of fully artificial DNA. During this synthesis, all nucleotides forming natural DNA are replaced by their modified versions with attached hydrophobic (i.e. water-repelling) molecules.</p>

Scientists from IOCB Prague are pushing the limits of DNA

<p><strong>Researchers from IOCB Prague are furthering the understanding of how medicines work and what it takes to develop their most effective variants. In one current study, they have focused on the disease caused by the protozoan parasite </strong><i><strong>Trichomonas vaginalis</strong></i><strong>, especially because of the recent appearance of strains that are resistant to conventional treatment. In an effort to find a new weak spot of this parasite, the </strong><a target="_blank" rel="noopener noreferrer" href="https://boura.group.uochb.cz/en"><strong>research group</strong></a><strong> led by Dr. Evžen Bouřa has succeeded in preparing a key enzyme complex – the proteasome. This has made it possible to gain knowledge that is indispensable for the development of new effective medicines. The research was carried out in collaboration with colleagues from the University of California San Diego. An article presenting the work has been published in the scientific journal </strong><i><strong>Nature Communications</strong></i><strong>.</strong></p><p>The proteasome is a complex protease that works as a device for recycling old proteins in cells. It is a cylindrical enzyme complex composed of seven subunits arranged in four superposed circles. When old proteins enter this cylinder, they get broken down into small pieces later used by the cell to make new proteins. If it functions incorrectly, old proteins accumulate, leading to the cell’s demise. This is why the blocking of proteasome activity is used, for example, against certain types of malignant tumours.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Tomas_Bellon_Vyzkum_vedcu_z_UOCHB_pomaha_vylepsovat_ucinky_lecivych_latek_Dr_Evzen_Boura_vedouci_vedecke_skupiny_Strukturni_biologie_membran_UOCHB_783ec20050.jpg" alt="IOCB Prague / Tomáš Belloň: Dr. Evžen Bouřa, head of the Structural Membrane Biology group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Tomas_Bellon_Vyzkum_vedcu_z_UOCHB_pomaha_vylepsovat_ucinky_lecivych_latek_Dr_Evzen_Boura_vedouci_vedecke_skupiny_Strukturni_biologie_membran_UOCHB_783ec20050.jpg 234w," sizes="100vw" width="1500" height="1001"></p><div><p>In the same way, it should also be possible to treat the widespread sexually transmitted infection trichomoniasis, which increases the risk of contracting HIV. The aim is therefore to identify substances that block the function of the proteasome inside <i>Trichomonas vaginalis</i> cells. However, the proteasome of this single-cell organism had never been satisfactorily isolated, so the research group from IOCB Prague prepared an artificial proteasome that is identical to the natural one. Curiously, they used insect cells to do so. This part of their research, on which the success of the whole project was hinged, proved the most complicated. That the team has prevailed in describing the previously unknown structure of the parasite’s proteasome is therefore of critical importance.</p><p><i>‘The proteasome looks the same in every animal or protozoan, but the details of this cylindrical structure differ. It is these details that we need to know in order to create a substance that is toxic to the parasite but not harmful to the human body. That is a crucial precondition for drug development. Our greatest success is that we have been able to prepare a recombinant, artificial proteasome that can provide us with the necessary information,’</i> explains Evžen Bouřa.</p><p>The researchers used advanced electron microscopy, allowing them to see almost every single atom. Armed with this capability, they tested the effects of two potential proteasome-inhibiting substances. They saw how they bind to the active site of the proteasome and under what circumstances the proteasome stops working, which is fatal to the cell of the parasite.</p><p>The research was carried out within the <a target="_blank" rel="noopener noreferrer" href="https://nivb.cz/en/">National Institute of Virology and Bacteriology</a> (LX22NPO5103), funded by the <a target="_blank" rel="noopener noreferrer" href="https://www.uochb.cz/en/exceles">EXCELES</a> program.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_vedcu_z_UOCHB_pomaha_vylepsovat_ucinky_lecivych_latek_1_8ff5bf4e71.jpg" alt="IOCB Prague: Dr. Evžen Bouřa, head of the Structural Membrane Biology group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_vedcu_z_UOCHB_pomaha_vylepsovat_ucinky_lecivych_latek_1_8ff5bf4e71.jpg 245w," sizes="100vw" width="1386" height="632"></p><p>&nbsp;</p></div>

Scientists from IOCB Prague help to improve medical drugs

<p><strong>In the week of October 14, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Metrohm: Electrochemical techniques for battery research and testing</strong></h5><ul><li><div><div>Tu, 15.10.2024 10:00 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4337">Registration</a></div></li></ul><p>Theoretical and practical aspects of various electrochemical techniques for battery research and testing.</p><h5><strong>2.&nbsp;Waters Corporation: Leveraging light scattering techniques for enhanced biotherapeutic characterization and development</strong></h5><ul><li><div><div>Tu, 15.10.2024 10:30 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4336">Registration</a></div></li></ul><p>How different light scattering techniques can be used for advanced characterization of biopharmaceutical samples.</p><h5><strong>3.&nbsp;Thermo Fisher Scientific: Solutions for environmental and food contaminant testing</strong></h5><div><ul><li><div>Tu, 15.10.2024 10:30 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4238">Registration</a></div></li></ul></div><p>New and advancing workflows to meet and exceed European regulations.</p><h5><strong>4.&nbsp;Agilent Technologies: Single Quad mass detection for Chromatographers</strong></h5><ul><li><div><div>Tu, 15.10.2024 11:00 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4261">Registration</a></div></li></ul><p>We will look at the development of single quads together and highlight the possibilities offered by today's single quads and how they support the user.</p><h5><strong>5.&nbsp;Agilent Technologies: MassHunter Acquisition Q-TOF Part II – Advance Settings</strong></h5><div><div><ul><li><div>Tu, 15.10.2024 14:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4343">Registration</a></div></li></ul></div><p>Join us for an in-depth exploration of advanced settings in MassHunter Acquisition for Q-TOF systems.</p></div><h5><strong>6.&nbsp;Thermo Fisher Scientific: High-throughput and automation in novel modalities (msAbs, ADCs, AOCs) screening, characterization, and beyond</strong></h5><ul><li><div><div>Tu, 15.10.2024 17:00 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4352">Registration</a></div></li></ul><p>Integrated advanced techniques such as automation and machine learning to achieve unprecedented mass spectrometry-focused sample handling capabilities and rapid turnover rates.</p><h5><strong>7.&nbsp;Phenomenex: Solid Phase Extraction Troubleshooting Tips and Tricks</strong></h5><ul><li><div><div>Tu, 15.10.2024 19:00 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4338">Registration</a></div></li></ul><p>Identify the common challenges associated with solid phase extraction methods.</p><h5><strong>8.&nbsp;Waters Corporation: Streamlined Proteomics with the Power of Andrew+</strong></h5><ul><li><div><div>We, 16.10.2024 11:00 CEST</div></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4339">Registration</a></div></li></ul><p>How the Andrew+ liquid handling robot has helped streamline their sample preparation workflows for proteomic analysis.</p><h5><strong>9.&nbsp;Agilent Technologies: Advancements in Host Cell Protein Analysis</strong></h5><div><ul><li><div>We, 16.10.2024 11:30 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4357">Registration</a></div></li></ul></div><p>Join us in this unique webinar where experts from Industry, pharmacopeia and Agilent will share insights on latest advancements on HCP analysis.</p><h5><strong>10.&nbsp;Waters Corporation: Free Testosterone and Free T3/T4</strong></h5><div><ul><li><div>We, 16.10.2024 14:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4356">Registration</a></div></li></ul></div><p>Simplified Equilibrium Dialysis and SPE Method for the UPLC-MS/MS Analysis of Free Thyroxine (FT4) and Triiodothyronine (FT3) for Clinical Research.</p><h5><strong>11.&nbsp;Technology Networks/Thermo Fisher Scientific: Streamlining Data for Superior Therapeutic Manufacturing</strong></h5><div><ul><li><div>We, 16.10.2024 17:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4377">Registration</a></div></li></ul></div><p>This webinar explores how to implement digitization to accelerate decision-making, meet regulatory demands and eliminate bottlenecks in biotherapeutic development.</p><h5><strong>12.&nbsp;Waters Corporation: Analyzing mRNA and sgRNA with Advanced Enzymes, Mass Spectrometry, and Informatics</strong></h5><div><ul><li><div>We, 16.10.2024 17:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4355">Registration</a></div></li></ul></div><p>This webinar will feature insights from Quality Assistance a leading European Contract Research Organisation (CRO) offering a range of services including mRNA and CRISPR sgRNA testing.</p><h5><strong>13.&nbsp;Separation Science/Merck: Empowering Tomorrow’s Therapeutics with LC-MS Capillary Chromatography</strong></h5><div><ul><li><div>Th, 17.10.2024 10:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4359">Registration</a></div></li></ul></div><p>This webinar emphasizes the significance of capillary HPLC coupled with mass spectrometry.</p><h5><strong>14.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 2</strong></h5><div><div><ul><li><div>Th, 17.10.2024 16:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4360">Registration</a></div></li></ul></div><p>This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data.</p></div><h5><strong>15.&nbsp;Waters Corporation: Nitrosamines</strong></h5><div><div><ul><li><div>Th, 17.10.2024 19:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3445">Registration</a></div></li></ul></div><p>This webinar will address analytical challenges in the detection and control of nitrosamines and Nitrosamine Drug-Substance Related Impurities (NDSRIs).</p></div>

Webinars LabRulezLCMS Week 42/2024

Article

Webinars

Scientific article

Science and research

Video

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Ústav organické chemie a biochemie AV ČR

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Metrohm

Mettler-Toledo

Agilent Technologies

Phenomenex

Waters Corporation

<ul>
<li><strong>Learn the various optical performance parameters</strong></li>
<li><strong>Learn which parameters need to be monitored</strong></li>
<li><strong>Benefits of automated performance qualification</strong></li>
<li><strong>Solution for data integrity compliance in optical instrument qualification</strong></li>
<li><strong>Overview of available service offerings from METTLER TOLEDO</strong></li>
</ul>
<p>During the webinar, we will go into depth about these questions and more:</p>
<h5><strong>What certified reference materials (CRMs) are specified for wavelength accuracy and precision tests by both Pharmacopeias?</strong></h5>
<p>USP: Rare earth oxide solutions/glasses (Ce, Ho, Didymium) or Hg/D2 emission lines</p>
<p>Ph. Eur.: Ho(ClO4)3, Ce2(SO4)3 solutions, or Ho/Didymium glasses, or Hg, D2, Ne, Xe lines</p>
<h5><strong>What CRMs are specified for photometric accuracy and precision tests by both Pharmacopeias?</strong></h5>
<p>USP: for UV range, Nicotinic acid in HCl (6 – 60 mg/L, up to 2.5 A, 213 and 261 nm) or K2Cr2O7 in HClO4 (6 – 200 mg/L, up to 3.0 A, 235, 257, 313, 350 nm); for visible range, neutral density glass filters (up to 3.5 A, 440, 465, 546.1, 590, and 635 nm)</p>
<p>Ph. Eur.: Suitable solid and liquid filters such as certified PDC or neutral density filters; or, instead of certified PDC, Nicotinic acid in HCl.</p>
<h5><strong>What CRMs are specified for photometric linearity tests by both Pharmacopeias?</strong></h5>
<p>USP and Ph. Eur., respectively, specify to use the materials for photometric accuracy tests also for photometric linearity tests.</p>
<h5>Target Audience</h5>
<p>Lab Managers, Lab Technicians, System Owners, Quality Assurance Managers, Quality Assurance Operators</p>
<blockquote>
<p><strong>Presenter:</strong> Dr. Fabian Müller (Product Manager SPG UV/VIS)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Hans-Joachim Muhr (Business Development Manager BioTech)</p>
</blockquote>


METTLER TOLEDO: Pharmacopeia – What to Check on UV Vis Spectrophotometers? 

Pharmacopeia – What to Check on UV Vis Spectrophotometers? 

USP and Ph. Eur. Requirements Placed on UV/Vis Spectrophotometers

Pharmacopeia – What to Check on UV Vis Spectrophotometers?

<p><strong>Contaminants in the sample matrix can create chromatography issues, system downtime, and unreliable test results. Having clean samples can help prevent most of these problems. There are many options for sample cleanup.</strong></p>
<p>We’ll discuss the following options:</p>
<ul>
<li><p><strong>Solid phase extraction (SPE), which provides the cleanest sample.</strong></p>
</li>
<li><p><strong>“Targeted” filtration, which can remove specific interferences from the sample matrix and provides clean samples.</strong></p>
</li>
</ul>
<p>In this talk, we’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.</p>
<blockquote>
<p><strong>Presenter:</strong> Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.</p>
</blockquote>
<blockquote>
<p>Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.</p>
</blockquote>
<blockquote>
<p>Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.</p>
</blockquote>


Agilent Technologies: Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

We’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.

Sample Cleanup: Method Development for SPE

<p><strong>Our team of experts will share tips and tricks, dive into practical recommendations and highlight selected applications such as IVT mRNA and long-read sequencing.</strong></p>
<p>Here’s a glimpse of what awaits you:</p>
<ul>
<li><p><strong>Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems</strong></p>
</li>
<li><p><strong>Best practices for IVT mRNA analysis using the Fragment Analyzer</strong></p>
</li>
<li><p><strong>Learn how to achieve a better prediction of long-read sequencing read lengths</strong></p>
</li>
</ul>
<p>Whether you’re a seasoned professional or just starting out, this event promises valuable takeaways!</p>
<blockquote>
<p><strong>Presenter:</strong> Samina Kaufmann (Product Specialist, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Leslie Friedmann (Product Manager, Agilent Technologies)</p>
</blockquote>


Agilent Technologies: Back to the Lab with Your Fragment Analyzer and Femto Pulse

Back to the Lab with Your Fragment Analyzer and Femto Pulse

Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems.

<p><strong>Titration is generally considered a fairly simple analytical technique. But what do you do, if your titration results are not what they should be? Enter Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters!</strong></p><p>In his webinar on September 18, Robert will show you what typical errors can occur during a titration – and how to avoid or remedy them. Focusing on troubleshooting, Robert will cover everything from the electrode to the setup of your titrator to titration parameters and calculation of &nbsp; the result. The following questions give you an idea of the scope and practical relevance of this webinar:</p><ul><li><strong>How important is calibration?</strong></li><li><strong>Do I really need to stir the titration solution – and why?</strong></li><li><strong>What do I do, if several equivalence points are detected – or none at all</strong></li><li><strong>How to clean the electrode properly?</strong></li></ul><p>These questions and many more will be answered step by step so that everyone can follow and understand. During a Q&amp;A session at the end of the webinar, you can ask Robert any question you may have about titration.</p><blockquote><p><strong>Presenter: </strong>Robert Tobler (Senior Application Specialist Titration, Metrohm International Headquarters)</p><p>After his apprenticeship as a chemical laboratory technician and further training as a biomedical analyst, Robert Tobler studied chemistry at the Zurich University of Applied Science in Waedenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since then, Robert has become an expert in applied titration sharing his expertise through the global network of Metrohm subsidiaries.</p></blockquote>

Metrohm: Troubleshooting titration: essential know-how for quality results

Troubleshooting titration: essential know-how for quality results

What typical errors can occur during a titration – and how to avoid or remedy them.

Troubleshooting titration: essential know-how for quality

<p><strong>This seminar will guide you through troubleshooting HPLC systems and chromatographic issues using a structured workflow.</strong></p><p>We will share a novel approach to emphasize the importance of system monitoring, as troubleshooting without monitoring is considerably more challenging. After introducing some key concepts and a troubleshooting workflow, we'll explore practical examples by applying the workflow to failed chromatograms to identify the cause of failure and then use a test cycle to confirm the fix.</p><p>Who should attend: Analysts working in regulated environments (eg. GLP, GMP) or in non-regulated environments (eg. R&amp;D) who want to troubleshoot their HPLC systems and chromatographic problems effectively.</p><h4>Key Learnings:</h4><p><strong>1. The importance of routine system monitoring</strong></p><p><strong>2. Using qualification checks to diagnose a failure</strong></p><p><strong>3. Understanding Failure Indicators (HOW the chromatogram failed) vs. Failure Causes (WHY the chromatogram failed)</strong></p><p><strong>4. Using troubleshooting workflows to quickly identify the failure cause</strong></p><blockquote><p><strong>Presenter: </strong>Maureen DeLoffi (Senior Product Manager, Waters COrporation)</p><p>Maureen DeLoffi is a Senior Product Manager at Waters Corporation, where she manages the small molecule portfolio of Analytical HPLC columns. Prior to her Product Manager role, she spent over 12 years as a scientist in the Column Technology Development Lab at Waters. She holds a bachelor’s degree in Mechanical Engineering from the University of New Hampshire.</p></blockquote>

Waters Corporation: HPLC Troubleshooting: A System Monitoring Approach

HPLC Troubleshooting: A System Monitoring Approach

Troubleshooting HPLC systems and chromatographic issues using a structured workflow.

<p><strong>The use of biotherapeutic synthetic peptides in drug development has grown significantly in the past decade.</strong></p><p>Biotherapeutic synthetic peptides often have higher efficacy, safety, and tolerability in humans compared to small molecules. &nbsp;They also have lower production complexity and cost than protein-based biopharmaceuticals.</p><p>While synthesis reactions are carefully controlled, impurities as well as degradation species are inevitably formed and must be characterized and identified. Having a robust Reversed-Phase (RP) method provides a solid foundation in the characterization and identification of these impurities. &nbsp;</p><p>This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.</p><ul><li>Principles of Reversed-Phase LC and LC/MS of Peptides</li><li>Considerations in Selecting an Appropriate RP Column</li><li>Considerations in Selecting an Appropriate RP Eluent</li><li>Step by Step Approach to Developing a Robust Peptide Separations</li></ul><blockquote><p><strong>Presenter:</strong> Bill Warren (Principal Consultant Product Manager, Waters Corporation)</p><p>In 1986, Bill joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids.</p><p>He currently is a Principal Consulting Bio separations Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.</p></blockquote>

Waters Corporation: Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.

RP Methods for the Analysis of GLP-1 Agonists

<p><strong>We are excited to bring you a three-part Ask Me Anything webinar series on one of the topics you ask about most – processing data. Empower Software allows you to use two different peak detection algorithms, Traditional and ApexTrack. What are the differences between these algorithms and when might you use one versus the other? Given all the choices on the Integration tab of the Processing Method, how do you set these important parameters to ensure accurate peak integration? &nbsp;</strong></p><p>In the first part of this webinar series, we will explore the algorithms and learn how to set the parameters in a logical fashion. <strong>We will discuss:</strong></p><ul><li>How both algorithms work</li><li>Starting with peak width and threshold first</li><li>Interactively setting timed events in the chromatogram</li><li>Practical examples</li><li>Empower System Policies that impact your peak detection and integration</li></ul><p>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p></blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 1

Empower Tips: Ask Me Anything About Processing Data - Part 1

We will explore the algorithms and learn how to set the parameters in a logical fashion.

Empower Tips: Ask Me Anything About Processing Data

<p><strong>USP &lt;621&gt; is a crucial chapter in the USP guidelines, affecting around 5000 monographs. It emphasizes the importance of establishing meaningful system suitability criteria for chromatography procedures to ensure valid, compendial, and regulatory compliance. The latest version of USP &lt;621&gt;, effective from December 2022, provides detailed guidance on method adjustments and transfers, facilitating the modernization of existing USP monograph methods. The revisions also expand the scope for modifying gradient method parameters and allow the transition from totally porous silica-based analytical columns to superficially porous particle-based columns to better meet industry needs.</strong></p><h5><strong>Key Learnings:</strong></h5><ul><li>Strategies for optimizing chromatographic performance using right HPLC system</li><li>Understand the latest updates in USP &lt;621&gt; and how they impact chromatographic practices</li><li>Techniques for enhancing laboratory productivity and reducing operational costs</li><li>Strategies to modernize chromatographic conditions with case-studies</li><li>Revised System suitability requirements and allowable chromatographic system adjustments</li><li>and more</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Manoj Kumar Singh (Senior Vice President, Microlabs Analytical Research Lab)</p><p>Dr. Manoj K Singh hold over 30 yrs of professional experience of working with global pharma companies leading their Analytical labs to support different pharma work-flows.</p><p>He holds a Ph.D. degree (Chemistry).</p><p>He started his professional career in 1993 with Wockhardt and spent significant tenure at major pharma R&amp;Ds like Lupin, Novartis, Orchid, Dabur &amp; Sentiss Pharma.</p><p>Currently he is associated with MicroLabs Bangalore as Senior Vice President (ADL) leading team of Analytical Chemistry scientists to support formulation development, Validations and method transfer.</p><p><strong>Presenter:</strong> Dr. Kuldeep Sharma (Sales Development Manager - Pharma, Agilent Technologies)</p><p>Kuldeep Sharma has over 19 years of experience in area/s of Bioanalytical research, Drug metabolism &amp; pharmacokinetics and Pharma regulatory compliance. He has accomplished PhD in Biotechnology. His professional journey covers over 12 years in Analytical research (associated with R&amp;D’s of Zydus Cadila, Lambda, Pliva and Jubilant Biosys) and last 7 years in analytical instruments sales, marketing &amp; business development. With over 21 research articles published in international Journals, He presents convention of continues learning.</p></blockquote>

Agilent: Enhance Lab Productivity While Meeting Regulatory Requirements: USP 621 Decoded

USP 621 Decoded

Enhance Lab Productivity While Meeting Regulatory Requirements

<h4>Key Learning Points:</h4><ul><li><strong>Identify the common challenges associated with solid phase extraction methods</strong><br>&nbsp;</li><li><strong>Understand and troubleshoot the recovery, contamination and reproducibility issues</strong><br>&nbsp;</li><li><strong>Learn how to find and apply solutions to develop robust and reproducible extraction methods for their sample preparation needs</strong></li></ul><h4>Who Should Attend:</h4><ul><li>Any scientists who wants to learn about solid phase extraction and it’s associated challenges, common troubleshooting steps and solutions to develop robust and reproducible extraction methods for their sample preparation needs</li></ul><blockquote><p><strong>Presnter: </strong>Namrata Saxena (Technical Manager, Phenomenex)</p><p>Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Sydney, Australia. As a member of the global technical team, she is responsible for assisting external customers as well as internal marketing and sales teams of Phenomenex for multifarious technical queries. She specifically support company’s bio-products portfolio through external and internal seminars/webinars, trainings and also contribute in biologics related technical content creation.</p><p>Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH.</p><p>Namrata has a post graduate degree with specialization in Analytical Chemistry. Her competence and focus lies in biotherapeutics characterization through liquid chromatography, antibodies and oligonucleotide chemistry, gene therapy and new bio-therapeutic modalities.</p></blockquote>

Phenomenex: Solid Phase Extraction Troubleshooting Tips and Tricks

Solid Phase Extraction Troubleshooting Tips and Tricks

Identify the common challenges associated with solid phase extraction methods.

<p><strong>This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data. The Components tab in the Processing Method displays many fields of information, giving you options about peak identification, calibration, reference peaks, and fields used in conjunction with custom fields.</strong></p><p><strong>In Part 2 of this series, we will discuss:</strong></p><ul><li>Peak identification</li><li>Calibration options including external and internal calibration</li><li>Reference Peaks for peak identification and calibration</li><li>Impurity fields</li><li>CCompRef1, 2, 3, and CConst1-7</li><li>Fields related to chemical structures</li></ul><p>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter: </strong>Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p></blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 2

Empower Tips: Ask Me Anything About Processing Data - Part 2

This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data.

LCMS webinars focusing on Tips & Tricks | LabRulez LCMS

Solid Phase Extraction Troubleshooting Tips and Tricks

Empower Tips: Ask Me Anything About Processing Data - Part 2

USP 621 Decoded

Back to the Lab with Your Fragment Analyzer and Femto Pulse

Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

HPLC Troubleshooting: A System Monitoring Approach

Pharmacopeia – What to Check on UV Vis Spectrophotometers?

Troubleshooting titration: essential know-how for quality results

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Empower Tips: Ask Me Anything About Processing Data - Part 1

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