<p><strong>In the  week from October 25, 2021, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">DETAILS and REGISTRATION can be found in the WEBINARS section.</a>:</strong></p>
<p><strong>Search and filter</strong> in more than <strong>1 200 GC and LC webinars</strong>.</p>
<h5><strong>1. PerkinElmer: LC-MS Tips &amp; Tricks</strong></h5>
<h5><strong>2. Phenomenex: How Column Handling and Storage Affects Performance and Lifetime</strong></h5>
<p>In this webinar we will cover HPLC columns chemistries and operating conditions, column life, washing procedures, regeneration procedures and storage conditions.</p>
<h5><strong>3. Agilent Technologies: High Throughput Workflows for Targeted Proteomics on QTOFs and QQQ</strong></h5>
<p>In this talk we explore high throughput techniques to do targeted proteomics. We will discuss peptide optimization with the Agilent optimization software and integrating Rapid Fire into the workflows.</p>
<h5><strong>4. Phenomenex: An Introduction to HPLC/ UHPLC Method Scaling</strong></h5>
<p>We will discuss Proper Method Scaling, The Effect of Particle Size vs. Efficiency, Flow Rate for the Intended Internal Diameter, Improving Efficiency and Scaling Towards Preparative Purifications.</p>
<h5><strong>5. Waters Corporation: Special on highly polar pesticides</strong></h5>
<p>Join us for our free Pesticide Thursdays webinar series and learn more on Waters turn-key solutions developed to tackle pesticide residues in or on food and feed.</p>
<h5><strong>6. Phenomenex: UHPLC Challenges and Solutions</strong></h5>
<h5><strong>7. SelectScience/Agilent Technologies: Sample prep strategies for the clinical research lab</strong></h5>
<p>Join Tina Chambers as she explores and compares sample preparation strategies for clinical research laboratories, ranging from simple dilute and shoot to solid-phase extraction (SPE). </p>
<h5><strong>8. Bruker Daltonics: ASMS 2021 - Bruker&#39;s Virtual eXceed Symposia - Expanding the Horizons of Single Cell Research</strong></h5>
<p>Welcome to Bruker&#39;s activities during ASMS 2021 where you will learn about all new product and software launched during 2021.</p>
<blockquote>
<h3><strong><a href="https://lcms.labrulez.com/webinars?search=RAFA">+ Overview of 13 LC &amp; LCMS on-line Webinars</a> at <a href="https://lcms.cz/events/53">RAFA 2021</a> conference</strong></h3>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_Overview_of_13_LC_and_LCMS_on_line_Webinars_at_RAFA_2021_conference_9c363e8f12/LabRulez-Overview-of-13-LC-and-LCMS-on-line-Webinars-at-RAFA-2021-conference.jpg" alt="LabRulez: Overview of 13 LC &amp; LCMS on-line Webinars at RAFA 2021 conference"/></p>


LC and LCMS webinars Week 43 + RAFA 2021 overview

<p><strong>In the  week from November 1, 2021, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">DETAILS and REGISTRATION can be found in the WEBINARS section.</a>:</strong></p>
<p><strong>Search and filter</strong> in more than <strong>1 200 GC and LC webinars</strong>.</p>
<h5><strong>1. Bruker Daltonics: ASMS 2021 -Bruker&#39;s Virtual Breakfast Workshop - Proteomics</strong></h5>
<p>Welcome to Bruker&#39;s activities during ASMS 2021 where you will learn about all new product and software launched during 2021.</p>
<h5><strong>2. Bruker Daltonics: ASMS 2021 - Bruker&#39;s Virtual Breakfast Workshop – Pharma/BioPharma</strong></h5>
<h5><strong>3. VWR: Chromatography Mini Symposium</strong></h5>
<p>This series of seminars covers a range of subjects from sharing best practice for reproducible chromatography to providing an introduction to the theory and practice of method translations.</p>
<h5><strong>4. Phenomenex: HPLC Mobile Phase Optimization</strong></h5>
<p>In this webinar we will cover how different reverse-phase organic solvents change selectivity, the role of pH and ionic strength in RP LC and when to use isocratic and gradient methods.</p>
<h5><strong>5. Bruker Daltonics: ASMS 2021 - Bruker&#39;s Virtual Breakfast Workshop– Imaging/SpatialOMx</strong></h5>
<h5><strong>6. SelectScience/Agilent Technologies: Achieving turnkey purification workflows on complicated drug modalities</strong></h5>
<p>Ed Ha, will showcase a collection of optimized workflows for small and large molecule quantification, and post-prep workflows, while providing top tips for identifying repetitive and unproductive steps.</p>
<h5><strong>7. Bruker Daltonics: ASMS 2021 - Bruker&#39;s Virtual Breakfast Workshop – Metabolomics</strong></h5>
<h5><strong>8. Food Quality &amp; Safety/Metrohm: Inline Dialysis - How to Make Sample Preparation for your Dairy Analysis More Efficient</strong></h5>
<p>Inline Dialysis and other inline sample preparation techniques are part of the Metrohm ion chromatography (IC) solution for dairy analysis.</p>
<h5><strong>9. SelectScience/Merck: Become a Drug Testing Wiz with MS ß-glucuronidases: Simplify your urinalysis workflow</strong></h5>
<p>Discover a simplified LC/MS urinalysis workflow using a stable ß-glucuronidase mix designed for robust urine drug testing of even the most challenging conjugated analytes.</p>
<blockquote>
<h3><strong><a href="https://lcms.labrulez.com/webinars?search=RAFA">+ Overview of 13 LC &amp; LCMS on-line Webinars</a> at <a href="https://lcms.cz/events/53">RAFA 2021</a> conference</strong></h3>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_Overview_of_13_LC_and_LCMS_on_line_Webinars_at_RAFA_2021_conference_9c363e8f12/LabRulez-Overview-of-13-LC-and-LCMS-on-line-Webinars-at-RAFA-2021-conference.jpg" alt="LabRulez: Overview of 13 LC &amp; LCMS on-line Webinars at RAFA 2021 conference"/></p>


LC and LCMS webinars Week 44 + RAFA 2021 overview

<p><strong>3. - 4. November 2021, RAFA 2021 completely VIRTUAL event this year. All activities will be held online and registration at RAFA 2021 is FREE OF CHARGE for all participants for both scientific program and webinars. We bring you [overview of 13 LC &amp; LCMS online Webinars] (https://lcms.cz/webinars?search=RAFA)</strong></p>
<p><strong>Search and filter in nearly 1,300 GC and LC webinars</strong>. </p>
<h5><strong>1. Shimadzu/RAFA 2021: Pitfalls in the Analysis of Mineral Oil Residues in Food</strong></h5>
<p>This talk will focus on mainly on the still present pitfalls of the analysis MOSH &amp; MOAH. </p>
<h5><strong>2. SCIEX/RAFA 2021: Advancements for food and environmental testing with the ZenoTOF 7600 system</strong></h5>
<p>In this webinar we will cover Novel MS technologies to elevate accurate mass sensitivity for MS/MS and the potential of a new fragmentation option for confirmation and identification.</p>
<h5><strong>3. Axel Semrau/RAFA 2021: Automated Solutions for the Analysis of MOSH/MOAH and Mycotoxins in Food</strong></h5>
<p>Focus will be on m-cpba-free epoxidation process and the current generation of GCxGC/TOF-MS measurement technology. The new system CHRONECT Symbiosis RIDA®CREST has been tested for Aflatoxin and Ochratoxin A.</p>
<h5><strong>4. PerkinElmer/RAFA 2021: Advancement in the determination and confirmatory analysis of PFAS in food matrices using QSight LC/MS/MS</strong></h5>
<p>Recent results on the development and validation of sensitive methods for determination and unambiguous confirmation of residues of PFAS in breast milk, retail milk, infant formulas and human serum.</p>
<h5><strong>5. Agilent Technologies/RAFA 2021: A Novel Workflow to Determine over 1000 Pesticide Residues in Compliance with SANTE 12682/2019 Guidelines in Various Food Matrices</strong></h5>
<p>We present a comprehensive, joint LC-MS and GC-MS workflow for the simultaneous quantitation of &gt;1000 pesticide residues in fruits and cereals of varying water and fat content.</p>
<h5><strong>6. Waters Corporation/RAFA 2021: A Simple, easy, and efficient way to process and review data for large multi residue methods using the MS Quan app within the new waters_connect software</strong></h5>
<p>We will demonstrate the usability of the software by generating a processing method from acquired data or the easy of setting up batch QC criteria and much more...</p>
<h5><strong>7. Restek Corporation/RAFA 2021: New Methods Targeting Emerging Food Contact Materials: MOSH/MOAH and PFAS</strong></h5>
<p>We will show you LC-GC×GC-ToFMS/FID for the quantitative and confirmatory analysis of MOSH and MOAH and the targeted approach for detecting and quantifying PFAS in paper and board matrices using LC/QQQ.</p>
<h5><strong>8. Bruker Daltonics/RAFA 2021: Use timsTOF Ion Mobility technology to improve results in Food analysis</strong></h5>
<p>We will discuss Using VIP-HESI and Trapped Ion Mobility Spectrometry to improve confidence in identification and workflow for setting up and controlling food authenticity analysis.</p>
<h5><strong>9. Agilent Technologies/RAFA 2021: Per- and Polyfluoroalkyl substances (PFAS): Analytical strategies to identify and quantify the next emerging contaminant in Food &amp; Beverage</strong></h5>
<p>This presentation will detail strategies for both targeted quantification and identification of legacy &amp; emerging PFAS in food &amp; beverage. </p>
<h5><strong>10. Waters Corporation/RAFA 2021: Enhancing analysis of foods using the ACQUITY™ Premier solution, Vitamin B, Organic Acids and beyond</strong></h5>
<p>We demonstrate improved performance in analyte peak area, limit of quantification, and carry-over using the MaxPeak™ HPS technology in the analysis phosphorylated and non-phosphorylated B vitamins.</p>
<h5><strong>11. Thermo Fisher Scientific/RAFA 2021: Taking advantage of the latest developments in GC- and LC- High Resolution Accurate Mass MS for both quantitative and non-target analysis in Food Safety</strong></h5>
<p>Find out how HRAM MS can be used to detect food fraud and how acquired data files can be used for both quantitation of target compounds and detection of unexpected compounds.</p>
<h5><strong>12. Phenomenex/RAFA 2021: Analysis of PFAS Compounds from Food and Food Contact Material</strong></h5>
<p>This webinar will give an overview of the PFAS workflows from foods and food contact material by focusing on PFAS extraction and analysis from various food samples, include eggs, fish, butter.</p>
<h5><strong>13. Phenomenex/RAFA 2021: Determination of Ionic Pesticides, Chlorate and Perchlorate in Various Food Matrices</strong></h5>
<p>This webinar will describe the performance of a new column, Venusil Hilic for the separation of ionic pesticides and inorganic anions chlorate and perchlorate.</p>


LC and LCMS webinars RAFA 2021

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 18th November 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications, presentations, and posters by <strong>Metrohm,</strong> <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu, Waters Corporation,</strong> and <strong>Agilent Technologies</strong>!</p><h4><strong>1. Agilent Technologies: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/why_use_hilic_and_hic_chromatography_oct082024_51637eb877.pdf"><strong>Why Use HILIC and HIC Chromatography?</strong></a></h4><ul><li>Presentation</li></ul><h5><strong>Agenda</strong></h5><ul><li><strong>What is HILIC and when should you consider it?</strong></li><li><strong>How to approach HILIC method development</strong><ul><li>Agilent HILIC column options</li><li>Mobile phase considerations</li></ul></li><li><strong>Common HILIC application areas</strong></li><li><strong>Tips and tricks for successful HILIC column use and care</strong><ul><li>Column equilibration</li><li>Sample solvent compatibility</li><li>Inert HILIC solution for metal-sensitive compounds</li></ul></li><li><strong>What is HIC and its separation mechanism?</strong></li><li><strong>Requirements for HIC</strong><ul><li>Factors affecting selectivity</li></ul></li><li><strong>Common HIC application areas</strong><ul><li>Post-translational modification (PTM) analysis</li><li>Separation of NIST mab (RM 8671)</li><li>Monitoring oxidation in mAbs</li></ul></li></ul><h5><strong>Summary</strong></h5><p><strong>When to consider a HILIC column:</strong></p><ul><li>Are your analytes unretained with RPLC?</li><li>Are your analytes at least somewhat soluble in acetonitrile?</li><li>Are you using MS detection?</li><li>Do your analytes interact with metals in the LC system?</li></ul><p><strong>Keep the sample solvent in mind for HILIC analyses:</strong> prepare the sample in as much acetonitrile as possible, and keep injection volumes as small as possible.</p><ul><li>Most common support issue with HILIC methods</li></ul><h4><strong>2. Shimadzu: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_06_saip_lc_077_en_e0b92b59d4.pdf"><strong>Simultaneous Quantitation of 32 PFAS in Baby Food by LC-MS/MS</strong></a></h4><ul><li>Application</li></ul><p><strong>User Benefits</strong></p><ul><li>A simple QuEChERS extraction procedure has been employed for quantifying the PFAS with normal dSPE cleanup.</li><li>This method complies with all acceptance criteria (as per AOAC SMPR 2023.003) without using ISTDs.</li><li>Method with shorter run time can contribute to achieving high throughputs in testing laboratories.</li></ul><p><strong>Introduction</strong></p><p>PFAS (Per- and polyfluoroalkyl substances) are a class of highly persistent chemicals widely known as "forever chemicals" due to their strong resistance to environmental degradation (Figure 1). Found in products like firefighting foams, non-stick cookware, and water-resistant fabrics, PFAS are now recognized as major environmental and health concerns. These substances accumulate in soil, water, and living organisms, including humans, where they may cause serious health impacts over time.</p><p>Given their potential for long-term contamination, the detection and quantification of PFAS are critical. Advanced analytical methods, particularly LC-MS/MS with Multiple Reaction Monitoring (MRM), offer the specificity and sensitivity needed to accurately measure PFAS concentrations. However, one of the primary challenges in PFAS analysis is minimizing background contamination from PFAS residues within the LC system and during sample collection and preparation. Effective strategies to reduce these sources of contamination are essential for reliable results in PFAS quantification.</p><p>n this application news, we describe a single laboratory validation study with a rapid extraction of trace level concentrations of 32 PFAS (Table 1) in infant formula (baby food) involving the use of the QuEChERS as an extraction technique followed by mass spectrometric analysis using a Shimadzu LCMS-8060NX triple quadrupole mass spectrometer coupled to a Shimadzu Nexera X3 liquid chromatograph (Figure 2).</p><p><strong>Conclusion</strong></p><ul><li>A simple, sensitive, and rapid LC-MS/MS method has been developed to quantify 32 PFAS compounds in infant formula.</li><li>This method utilizes a QuEChERS-based extraction technique for efficient sample preparation.</li><li>With Shimadzu’s LC-MS/MS, the method demonstrated high sensitivity and reproducibility, achieving low relative standard deviations (RSDr and RSDR) in accordance with AOAC SMPR standards at trace levels.</li><li>This approach offers a reliable solution for multi-PFAS analysis in infant formula, making it highly applicable for testing laboratories focused on food safety.</li></ul><h4><strong>3. Thermo Fisher Scientific / </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/170"><strong>RAFA</strong></a><strong>: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/PFAS_analysis_strategy_story_direct_injection_DLLME_LC_MS_MS_LC_Orbitrap_GC_Orbitrap_7592743c1a.pdf"><strong>PFAS analysis strategy story – direct injection, DLLME, LC-MS/MS, LC- Orbitrap / GC-Orbitrap</strong></a></h4><ul><li>Poster / <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/170"><strong>RAFA</strong></a></li></ul><h5><strong>Abstract</strong></h5><p><strong>Purpose:</strong> This work summarizes key components of PFAS analysis. The choice between direct injection and automated sample preparation depends on the instrument's dynamic range, detection limits, and matrix complexity. Complex matrices like waste/industrial water, soil, food, and biological samples require specific preparation techniques to mitigate matrix effects and ensure accurate quantification. Techniques such as Dispersive Liquid-Liquid Microextraction (DLLME) can significantly improve concentration factors and reduce matrix interference, enhancing overall method performance. Additionally, the choice of the<br>analyzer is a critical consideration.</p><p><strong>Methods: </strong>Direct injection of water sample was performed on Thermo Scientific TSQ Altis Plus triple quadrupole mass spectrometer. For those sample prepared with automated dispersive liquid-liquid microextraction (DLLME), acquisition was performed on a Thermo Scientific Orbitrap Exploris MX high resolution mass spectrometer.</p><p><strong>Results: </strong>The methods presented achieves high-level sensitivity for PFAS analysis in drinking water, detecting compounds in the low ng/L range. Using DLLME, 56 PFAS compounds were quantified to low part per trillion levels from just 15 mL of sample. With a FAPAS®-Drinking Water proficiency test, we validated the<br>three strategies for PFAS analysis: direct injection on TSQ Altis Plus and the DLLME extract injected on both Orbitrap Exploris MX and Thermo Scientific Orbitrap Exploris GC.</p><h5><strong>Conclusions</strong></h5><ul><li>Compared to traditional SPE workflows, direct injection improves sample throughput in laboratories by significantly reducing the required sample preparation.</li><li>The DLLME method is a promising technique for extracting and pre-concentrating PFAS from drinking water and various other matrices. Its reduced sample volume facilitates easier handling, transportation, and storage.</li><li>Both methods lower costs and environmental impact due to minimal solvent usage and the elimination of filters or SPE cartridges.</li><li>The ease of use and robustness of both LCMS methods are based on fixed configuration including SOP with detailed hardware and consumables, a complete acquisition and processing method with customized view settings and reports, and all data handling performed with Chromeleon CDS 7.3.2.</li></ul><h4><strong>4. Waters Corporation / </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/303"><strong>HPLC</strong></a><strong>: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_hplc_zabala_cortecs_solid_core_scalability_3ffe31ac22.pdf"><strong>SCALABILITY OF SOLID-CORE PARTICLES FOR CHROMATOGRAPHIC ANALYSES</strong></a></h4><ul><li>Poster / <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/303"><strong>HPLC</strong></a></li></ul><p><strong>NTRODUCTION</strong></p><p>Liquid Chromatography plays a crucial role in many areas of science. It is widely used for quality control of pharmaceuticals and biologics, research and medical purposes (1). Over the last several decades the gold standard of analysis for these areas of science has been High-Performance Liquid chromatography (HPLC). Typical HPLC systems operate within a 6000 psi pressure limit (2). Consequently, they are commonly paired with columns packed with 3 - 5 μm particles. Although smaller particle-packed columns allow faster separations, their use in HPLC is restricted to shorter lengths due to elevated backpressures (3). Additionally, established methods which are often used in a quality control setting may restrict alterations to particle size or column configuration.</p><p>Improved column efficiency on an HPLC system can be achieved using solid-core particles. Solid-core particles have a non-porous “solid” center and a porous outer layer(4). Columns packed with solid-core particles offer distinct advantages over those packed with fully porous particles, including higher efficiency and reduced operating backpressure. Waters offers a line of solid-core particle columns known as CORTECS Columns.</p><p>By utilizing CORTECS Columns, researchers can increase separation efficiency, achieve faster analysis times, reduced solvent waste and decreased column back<br>pressure. In this poster, we delve into the significance of larger solid-core particles for scalability. We compare columns packed with 5 μm CORTECS Prototype Material, CORTECS UPLC μm) Material and CORTECS HPLC (2.7 μm) C18 Material with columns packed with fully porous particles. Additionally, we explore how CORTECS Columns differ from similar offerings on the market.</p><p><strong>CONCLUSION</strong></p><ul><li>5 μm CORTECS Prototype Columns have 28% lower back pressure in comparison to 3.5 μm XBridge BEH C18 Columns.</li><li>5 μm CORTECS Prototype Columns have 14% higher optimal efficiency in comparison to 3.5 μm XBridge BEH C18 Columns.</li><li>CORTECS Columns are scalable across all 3 particle sizes shown.</li><li>In comparison, Vendor H and A columns are not scalable across all 3 particle sizes due to the significantly lower retention in the 4 and 5 μm particle size columns, shown in Figure 3.</li></ul><h4><strong>5. Metrohm: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_bat_015_download_c51bc5f0fe.pdf"><strong>Differential capacity analysis (DCA) for battery research with INTELLO</strong></a></h4><ul><li>Application</li></ul><p><strong>Introducing dQ/dE plots, applications, and more</strong></p><p>Differential capacity analysis (DCA) is a powerful diagnostic technique widely used in battery research and development to gain detailed insights into the electrochemical behavior of batteries. DCA allows researchers to identify key electrochemical processes, phase transitions, and degradation mechanisms occurring within the battery during charge and discharge cycles. This technique is particularly valuable for characterizing complex systems such as lithium-ion batteries, where multiple electrochemical reactions may overlap.</p><p>This Application Note explores the principles and practical applications of differential capacity analysis, highlighting its role in improving battery performance.<br>This application also reveals how INTELLO, with its dedicated suite of battery-oriented commands and its ability to untether during long measurements, is an excellent choice for battery researchers looking to maximize their time and efficiency when conducting DCA.</p><p><strong>INTRODUCTION</strong></p><p>Within the INTELLO battery cycling environment, it is possible to sample the differential capacity (defined as dQ/dE = |Qn+1-Qn|/(En+1-En), where n is the index of the data point, Q is the value of the charge/discharge capacity, and E is the measured working electrode potential) and plot by default this value versus the measured voltage of the cell. Note that a general introduction to the cycling environment in INTELLO is provided in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_bat_014_download_6c810739bf.pdf"><strong>AN-BAT-014</strong></a> while this Application Note specifically focuses on the dQ/dE plot and what can be derived from it.</p>

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News from LabRulezLCMS Library - Week 47, 2024

LC and LCMS webinars RAFA 2021

LC and LCMS webinars Week 44 + RAFA 2021 overview

LC and LCMS webinars Week 43 + RAFA 2021 overview

Upcoming events

Understanding quantification algorithms

36th MassSpec Forum Vienna 2026

Analytical Strategies for Peptide & Protein Therapeutics

Webinars

High-throughput stability screening strategies for antibody formulation

Big Scale, Big Wins: Prep Chromatography Explained

PFAS in Food & Food Contact Material