<h4>Welcome to “Analytically Speaking,” the podcast from LCGC International and Spectroscopy.</h4>
In this episode, podcast host Dr. Dwight Stoll talks with Dr. Katelynn Perrault Uptmor, <a href="https://gcms.labrulez.com/articles?search=Pierre-Hugues+Stefanuto">Dr. Pierre-Hugues Stefanuto</a>, and <a href="https://gcms.labrulez.com/articles?search=Petr+Vozka">Dr. Petr Vozka</a> about the multidimensional chromatography workshop, better known as the MDCW for short. Kate, PH, Petr, and Dwight are co-organizers of the MDCW workshop, which was held most recently at California State University, Los Angeles.
In their conversation they discuss how the MDCW is different from, and similar to, conventional scientific conferences. They reflect on <a href="https://gcms.labrulez.com/events/436">the most recent (15th) meeting</a>, including highlights from both the technical and social programs, and in the impact on the local student community at Cal State LA. Then, they switch gears and look forward to <a href="https://gcms.labrulez.com/events/518">the 16th MDCW</a>, which will be held February 3-5 at the University of Liege, in Liege, Belgium.
<blockquote>
Audio 🎧 <a href="https://soundcloud.com/analytically-speaking/ep-22-around-the-world-with-the-multidimensional-chromatography-workshop">Analytically Speaking: Ep. 22: Around the world with The Multidimensional Chromatography Workshop</a> 
</blockquote>
<h5>Resources Discussed</h5>
<ul>
<li><a href="http://www.multidimensionalchromatography.com/">MDCW Website</a></li>
<li><a href="https://labrulez.com/">LabRulez Website</a></li>
</ul>
<blockquote>
💡 Recordings of lectures available at <a href="https://www.youtube.com/playlist?list=PLg-M6fM-05MrGrDggkl_UKzzcHPWPmssh">YouTube playlist The Multidimensional Chromatography (MDC) Workshop</a>
</blockquote>
<blockquote>
💡 Further information can be found on <a href="https://gcms.labrulez.com/companies/218">The Multidimensional Chromatography (MDC) Workshop profiles on LabRulezGCMS and LabRulezLCMS portals</a>
</blockquote>
<h4>More about our hosts:</h4>
<h5>Dwight Stoll, PhD:</h5>
Dwight R. Stoll is a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota. He received his PhD from the University of Minnesota, under Professor Peter Carr, working on the development of fast, comprehensive two-dimensional liquid chromatography (2D-LC). Stoll’s current primary research focus is on the development of 2D-LC for both targeted and untargeted analyses. Active research projects in his laboratory touch on most aspects of multidimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase LC, instrument development, and applications in biopharmaceutical analysis. Stoll is the author or co-author of more than 80 peer-reviewed publications and six book chapters and has instructed numerous short courses in 2D-LC. In 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2017 he received the Georges Guiochon Faculty Fellowship, and was recognized with an Agilent Technologies Thought Leader Award. He is also a member of LCGC’s editorial advisory board and is the editor of the “LC Troubleshooting” column in LCGC.
<h5>Jerome Workman, Jr., PhD:</h5>
Jerome (Jerry) J. Workman, Jr. is the Senior Technical Editor for LCGC and Spectroscopy. He has held positions as CTO, executive VP, senior research fellow, director, and senior scientist at companies of all sizes, from start-ups to world-leading corporations. He has been an adjunct faculty member of four universities and advised multiple graduate students. He has more than 75 U.S. and international patent applications and 30 issued U.S. and international patents and multiple trade secrets, as well as 500+ technical publications, and 20 reference book volumes on a broad range of spectroscopy and data processing techniques. He has received multiple awards from scientific societies, and has taught annual courses in spectroscopy, chemometrics, and statistics for the AOAC, ACS, ISA, FACSS, and at several universities and corporations. He is a Fellow of the American Institute of Chemists (FAIC), the American Society for Testing and Materials (ASTM), and the Royal Society of Chemistry in the UK (FRSC, CChem, CSci). Jerry holds B.A and M.A degrees from Saint Mary&#39;s University of Minnesota, and a PhD degree from Columbia Pacific University working in near-infrared spectroscopy. He is an alumnus of both Columbia University Business School and the MIT Sloan School of Management.
<h4>About the Analytically Speaking Podcast:</h4>
Analytically Speaking, the podcast from LCGC and Spectroscopy, addresses important issues in separation science and analytical spectroscopy. Topics include new analytical techniques, methods, and approaches; the latest trends; advances in instrument and software technology; practical solutions for specific applications; recent papers in the scientific literature and their applicability; challenges and solutions for data analysis and interpretation; analytical chemistry theory and fundamentals (from advanced research to tutorials and troubleshooting); and more. Our regular hosts are Dwight Stoll, PhD, a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota, and Jerry Workman, PhD, a spectroscopist, noted author, and currently the Senior Technical Editor of Spectroscopy and LCGC. Dwight covers separation science and Jerry addresses spectroscopy related topics.

Around the world with The Multidimensional Chromatography Workshop

Los Angeles, CA – This January, Cal State LA became a hub for scientists and researchers as it hosted the 15th Multidimensional Chromatography Workshop. From January 10 to 12, 2024, the event brought together leading experts and those new to the field of multidimensional chromatography, focusing on the latest techniques and developments.
Organized by Drs. Katelynn Perrault Uptmor (William &amp; Mary), Pierre-Hugues Stefanuto (University of Liège), Dwight Stoll (Gustavus Adolphus College), and Petr Vozka (California State LA), the workshop featured talks and presentations on key trends in multidimensional gas and liquid chromatography from both academic and industry leaders.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Complex_Chemical_Composition_Analysis_Lab_C_AL_Cal_State_LA_hosts_15th_Multidimensional_Chromatography_Workshop_2_a9147b68cc.jpg" alt="Complex Chemical Composition Analysis Lab (C³AL): Cal State LA hosts 15th Multidimensional Chromatography Workshop."/>
The workshop featured keynote and contributed presentations and discussion groups on important topics like new technology, data analysis, and practical applications in various fields. A highlight was the poster session, where researchers competed for awards sponsored by the American Chemical Society, showcasing the workshop&#39;s role in supporting new scientific discoveries.
Thanks to the generous backing of sponsors such as LECO, JEOL, SepSolve Analytical, GC Image, Spectral Works, RESTEK, Thermo Fisher, TRAJAN, VICI DBS, Chemistry Matters and the Cal State LA Scholarship, and Creative Activities, along with CREST CATSUS and Sikand SITI Center, admission to the workshop was free.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Complex_Chemical_Composition_Analysis_Lab_C_AL_Cal_State_LA_hosts_15th_Multidimensional_Chromatography_Workshop_3_4c2502883b.jpg" alt="Complex Chemical Composition Analysis Lab (C³AL): Cal State LA hosts 15th Multidimensional Chromatography Workshop."/>
Dr. Krishna Foster, Chair of the Department of Chemistry and Biochemistry, opened the event with a welcoming speech that underscored the importance of chromatography in research, particularly in her own field of atmospheric chemistry. She highlighted California State University, Los Angeles&#39;s unique character as a &quot;people&#39;s university&quot; that champions diversity, equity, and nurturing talent from all backgrounds. Dr. Foster&#39;s speech set the tone for the event and spotlighted the university&#39;s commitment to sustainability and the integration of natural and social sciences.
Special thanks are extended to the students of <a href="https://gcms.labrulez.com/companies/219">C3AL</a> for their invaluable assistance during the event.
The conference is held annually, and further information about future events can be found at <a href="www.multidimensionalchromatography.com">www.multidimensionalchromatography.com</a>
<h4>Presentations from the 15th Multidimensional Chromatography Workshop were recorded and are available online.</h4>
Watch the <a href="https://www.youtube.com/playlist?list=PLGv1zjkq8eaB87EPMLGrAm473bAmsgbjj">whole playlist</a> or follow the links below for particular lecture.
<ul>
<li><a href="https://www.youtube.com/watch?v=mYxG6SdiiyQ">GC×GC-TOFMS/FID hydrocarbon group-type analysis of plastic-derived oils (Christina Kelly, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=aDa3pApEPKY">Multi-omics workflow for blood serum screening using GC×GC-TOFMS (Pierre-Hugues Stefanuto, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=PxVRSslhoF4">Off-line LC×SFC-HRMS/MS non-target analysis of depolymerised lignin (Eliise Tammekivi, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=Q2U73RcxnRk">Alkene quantification in plastic waste-derived fuels using GC×GC-FID (Genesis Barzallo, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=4vk8bUGHwGM">Streamlining group type analysis with GC×GC templates V-A alignment (Daniel Geschwender, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=scQGzl54RAs">Don’t let the realityof GC×GC-MS data burst your bubble! (James Harynuk, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=DtHpfwil5nQ">Chemical characterization of the aircraft cabin environment by GC×GC-TOFMS (Kevin Hayes, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=pWMEkg4Yn6c">Sampling body odor for healthcare monitoring: how to avoid the pitfalls (Elsa Boudard, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=_N8urY1yl8I">Exploring arson investigations with multi-dimensional chromatography (Gwen O&#39;Sullivan, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=Qo9ywERlTH4">Automated online multicolumn 2D-LC screening as a rapid and efficient tool (Heather Wang, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=y7P6PJ_K8Sk">Extending boiling point range using PLOT columns and GC×GC-MS (Lina Mikaliunaite, MDCW 2024)</a>
</li>
<li><a href="https://www.youtube.com/watch?v=SpsGHl5erqw">Sensory-classified roasted arabica coffee with GC×GC-TOFMS and chemometrics (Caitlin Cain, MDCW 2024)</a>
</li>
<li><a href="https://gcms.labrulez.com/companies/218">Expanding the scope of tile-based GC×GC–TOFMS data analysis (Robert Synovec, MDCW 2024)</a>
</li>
<li><a href="https://gcms.labrulez.com/companies/218">Changes validate breath signatures of acute respiratory exacerbations (Michael Wilde, MDCW 2024)</a>
</li>
</ul>

Cal State LA hosts 15th Multidimensional Chromatography Workshop

News from LabRulezLCMS Library - Week 14, 2025

In the realm of analytical chemistry, particularly in the specialization of chromatography, the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/companies/218">Multidimensional Chromatography Workshop (MDCW)</a> stands out as a benchmark event. Hosted annually, this workshop not only showcases innovations in multidimensional chromatography but also fosters a deeply collaborative and inclusive scientific community. This article presents a structured overview of the key highlights and offerings of MDCW, based on insights shared by Dr. Petr Vozka (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/219">Cal State LA's Complex Chemical Composition Analysis Lab (C³AL)</a>) in an interview with Organomation.<h4>What Is MDCW?</h4>The Multidimensional Chromatography Workshop (MDCW) is a technically focused, research-driven conference dedicated to advancing chromatography practices. The event brings together scientists from around the world to explore topics such as:<ul><li>Gas Chromatography (GC)</li><li>Liquid Chromatography (LC)</li><li>Supercritical Fluid Chromatography (SFC)</li></ul>Unlike many commercial conferences, MDCW is known for its academic rigor, collaborative environment, and comprehensive educational sessions.<h4>Key Features of MDCW</h4><h5>Free Registration and Generous Support</h5>One of the standout aspects of MDCW is its completely free registration, made possible by the generous sponsorship of industry and academic partners. This eliminates financial barriers for students and early-career researchers.As Dr. Petr Vozka emphasized:“The registration is completely free, thanks to the generous support of our sponsors. Attendees also get lunch, dinner, coffee, and drinks every day.”<h5>Global Accessibility</h5>MDCW alternates locations between Europe and the United States, enhancing accessibility for international researchers. This geographical rotation ensures that:<ul><li>Scientists from multiple continents can participate over time.</li><li>Institutional and regional barriers to access are minimized.</li></ul><h5>Comprehensive Coverage of Chromatographic Techniques</h5>The conference is not limited to one method. It incorporates presentations and discussions on GC, LC and SFC. This broad scope ensures that MDCW remains a key venue for cutting-edge separation science.<h4>Community and Collaboration</h4><h5>An Atmosphere of Support and Innovation</h5>MDCW is highly regarded for its welcoming and collaborative culture. Attendees often form long-term professional relationships and research collaborations that persist beyond the event.As Dr. Vozka described:“The atmosphere is incredibly supportive and collaborative. People genuinely root for each other, and many long-term partnerships grow out of these interactions.”<h5>Real-World Collaborations</h5>Dr. Vozka shared a personal example of the value of these connections:“I met <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/articles?search=Katelynn+Perrault">Dr. Katelynn Perrault </a>at my first MDCW. We started collaborating and now she’s one of my mentors. We talk almost regularly about research.”<h4>Commitment to Open Access and Digital Sharing</h4><h5>Recordings and Posters Available Online</h5>MDCW’s commitment to accessibility extends beyond the physical event. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/218">All presentations and posters are made available online</a> via the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/companies/1">LabRulez</a> platform, contributing to the global exchange of scientific knowledge.<ul><li>Open-access approach encourages knowledge sharing.</li><li>Attendees can revisit sessions and share content with peers.</li></ul>Note: Presenters who prefer not to share their data publicly are fully respected, and their materials are not posted without consent.<h4>Growing Popularity and Future Outlook</h4><h5>A Rapidly Expanding Event</h5>The most recent edition of MDCW saw record attendance and abstract submissions, indicating its rising prominence and influence in the field of multidimensional chromatography.“This past year was especially exciting,” said Dr. Vozka. “It shows how fast the conference is growing.”<h4>Upcoming Event Details</h4>The <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/787">17th MDCW</a> is scheduled for:<ul><li>📅 Date: January 13–15, 2026</li><li>📍 Location:&nbsp;Williamsburg, Virginia, USA</li></ul>With its established reputation and growing community, MDCW 2026 is expected to deliver another impactful event.<h5>How to Get Involved</h5><ul><li>Visit the official website: <a target="_blank" rel="noopener noreferrer" href="https://www.multidimensionalchromatography.com">MultidimensionalChromatography.com</a></li><li>Explore previous content: Watch <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/playlist?list=PLg-M6fM-05MrGrDggkl_UKzzcHPWPmssh">session recordings</a> and browse <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=366">poster archives</a> on YouTube and the website of LabRulez</li><li>Engage with the community: Follow MDCW updates on academic and social media platforms.</li></ul><h4>Conclusion</h4>The Multidimensional Chromatography Workshop (MDCW) is more than just a scientific meeting — it is a community of scholars and practitioners committed to advancing separation science in a collaborative, inclusive, and highly accessible manner.From the zero-cost registration to the atmosphere of open mentorship and the global reach of its content, MDCW offers unmatched value to researchers at all stages of their careers.Whether you're a student just beginning your journey or an established expert seeking meaningful collaborations, MDCW is a must-attend event.<blockquote><h5>About the Speaker</h5>Dr. Petr Vozka is an expert in chromatography and a returning organizer and participant at MDCW. His experience and enthusiasm for the workshop reflect the values of inclusivity, knowledge sharing, and scientific advancement that define the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Petr_Vozka_1329d3773e.jpg" alt="VŠCHT: Petr Vozka" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Petr_Vozka_1329d3773e.jpg 143w," sizes="100vw" width="917" height="1000"></blockquote>

Why MDCW Is the Premier Conference in Chromatography

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 7th July 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application note by Agilent Technologies and posters by Shimadzu / ASMS, Thermo Fisher Scientific / ASMS and Waters Corporation / ASMS!<h3>1. Agilent Technologies: Rapid and Sensitive Analysis of Antifungal Agents in Human Serum Using the Agilent 6475 LC/TQ</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_antifungal_human_serum_5994_8338en_agilent_89f89cb4dc.pdf">Full PDF for download</a></li></ul>In addition to minimizing drug-related toxicity, TDM has been hailed as a key step to prevent the emergence of drug resistance. The growing number of invasive fungal infections creates a ripple effect on the rapid advancement of TDM for antifungal agents. Increasing evidence confirms the critical role of TDM in preventing and treating antifungal infections.1 The RECIPE ClinMass TDM kit system for antimycotics in serum (Munich, Germany) was used on a 6475 LC/TQ for the analysis of eight antifungal agents. This application note demonstrates the evaluation and validation of the RECIPE TDM kit, covering sensitivity, linearity, and robustness across five consecutive days (n =&nbsp;5). Quick and simple sample preparation was implemented, providing high-throughput monitoring of antifungal agents in biological&nbsp;samples.<h4>Experimental</h4><h5>Instrumentation</h5><ul><li>Liquid Chromatography System&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=1&amp;search=Agilent+1290+Infinity+II">Agilent 1290 Infinity II</a> High-Speed Pump&nbsp;</li><li>Agilent 1290 Infinity II Multisampler&nbsp;</li><li>Agilent 1290 Infinity II Multicolumn Thermostat&nbsp;</li></ul></li><li>Mass Spectrometry System&nbsp;<ul><li>Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=1&amp;search=6475+LC%2FTQ">6475 LC/TQ &nbsp;</a></li><li>Agilent Jet Stream Technology Ion Source (AJS)</li></ul></li></ul><h5>Method&nbsp;</h5>The optimization of multiple reaction monitoring (MRM) transitions for analytes and ISTDs was carried out using Auto MRM Optimizer in the Agilent MassHunter acquisition software to provide optimal sensitivity and selectivity. Data were processed using the Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=1&amp;search=MassHunter+Qualitative+Analysis+software">MassHunter Qualitative Analysis software</a>, version B.12, and Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=1&amp;search=MassHunter+Quantitative+Analysis+software">MassHunter Quantitative Analysis software</a>, version B.12.<h4>Results and discussion&nbsp;</h4>Figure 2 shows the chromatograms of quantifier MRM transitions for the eight antifungal agents at the lowest calibration level. Six out of eight antifungal agents had good chromatographic separation across the run time. The retention times of two antifungals, posaconazole and hydroxy-itraconazole, were slightly overlapped. Despite this overlap, measurement integrity can still be maintained due to MRM selectivity.<h4>Conclusion&nbsp;</h4>A fast and sensitive analysis of eight antifungal agents in serum was validated on an Agilent 6475 triple quadrupole LC/MS. By employing a ready-to-use RECIPE ClinMass TDM kit, this method demonstrates accurate and precise quantitation of antifungal agents in biological samples. Additionally, the streamlined sample preparation method allows high throughput analysis of up to 350 samples per day.<h3>2. Shimadzu / ASMS: Robustness Evaluation of PFAS Analysis in Soil Using LC-MS/MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ASMS_25_WP_137_e932b391c7.pdf">Full PDF for download</a></li></ul>Per- and polyfluoroalkyl substances (PFAS) are widely used in various fields and industries. However, due to their structural stability and resistance to degradation, they accumulate in the environment and are suspected of being harmful to humans. PFAS in soil can accumulate in the human body via agricultural products, thus it is essential to develop sensitive and robust analytical methods for the detection of PFAS in soil with complex matrices.1 This study shows a robustness evaluation by adding 30 PFAS to a soil matrix and using the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=3&amp;search=LCMS-8060RX">LCMS-8060RX</a> to measure these PFAS in the soil matrix 500 consecutive times.<h4>Methods&nbsp;</h4>For this analysis, LC-MS/MS equipped with a newly designed ESI source was used, and a delay column was installed between the mixer and the autosampler to inhibit the effects of PFAS contamination from the LC system. Six points calibration curves were built, and each calibration point was measured 3 times. A soil sample was prepared referring to part of the soil preparation procedures published by the National Agricultural and Food Research Organization. 2 Thirty PFAS compounds were spiked to the soil sample at 0.1 μg/L after pretreatment and 500 soil sample analyses were performed for the robustness evaluation. This sample contains more than 90% soil matrix. QC samples were also analyzed after every 20 injections of the soil samples.<h4>Conclusion</h4>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=3&amp;search=LCMS-8060RX">LCMS-8060RX</a> performed 500 consecutive measurements of 30 PFAS spiked to a soil matrix sample. The results showed good peak area repeatability, good peak shapes, and good recovery. The newly designed ESI source showed excellent robustness, and stable analysis was achieved over a long period, even for samples with complex matrices.<h3>3. Thermo Fisher Scientific / ASMS: Evaluation of a modified Orbitrap Astral Mass Spectrometer for label-free quantitation of proteomic samples</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/PO_439_ms_ASMS_25_Astral_Zoom_LFQ_po439_na_en_c1a0b2a11e.pdf">Full PDF for download</a></li></ul>The demand for high-throughput proteomics analysis of complex samples is continually increasing, focusing on greater analysis depth and improved precision and accuracy in quantitation. Label-free quantitation of very complex proteomic samples poses significant challenges for mass spectrometers, requiring faster and more efficient processing of eluting peptide ions and their fragments. The new Orbitrap Astral Zoom mass spectrometer addresses these challenges, offering a higher acquisition rate by improved ion optics switching times and faster ion transfer, higher sensitivity due to ion pre-accumulation, and enhanced spectral processing, leading to deeper coverage with higher confidence.<h4>Materials and methods</h4><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo</a>: Direct injection mode using 25cm x 75 μm ID Aurora Ultimate TS column (IonOpticks)&nbsp;</h5><ul><li>High loads: 37min gradient; 4-45%B and injection-to-injection time 48min (30SPD)&nbsp;</li><li>Low loads: 20min gradient; 1-40%B and injection-to-injection time 28min (50SPD)&nbsp;</li></ul><h5>Mass Spectrometer: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/836">Orbitrap Astra</a>l and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom&nbsp;</a></h5><ul><li>Orbitrap (MS1) and Astral (MS2) detectors&nbsp;</li><li>Orbitrap: 240K; m/z range 380-980; AGC 500%; max IT 5ms&nbsp;</li><li>NCE 25; Thermo Scientific FAIMS Pro Duo (low loads): -48V; gas 3.5 L/min</li><li>Astral: DIA; window size depending on loads (in the tables below); m/z 150-2000; AGC 800% (low loads) or 100% (high loads); max IT depending on load; cycle time: 0.6s&nbsp;</li></ul><h5>Data Processing&nbsp;</h5><ul><li>Spectronaut® v19.5 Direct DIA, 6 files (2 x 3 replicates) processed together for each load; all other settings were default&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Thermo Scientific Proteome Discoverer software </a>3.3.0.54, CHIMERYS 4.0.21</li><li>Human, E.coli, and Yeast FASTA (all w/o isoforms) from UniProt; normalization by Human FASTA</li></ul><h4>Results (High Loads)</h4>The new Orbitrap Astral Zoom mass spectrometer takes advantage of ion preaccumulation in the bent trap, increasing the effective MS2 injection time. Its reduced ion transfer overheads result in a higher MS2 acquisition rate of up to 270 scans per second. Additionally, an enhanced spectral processing algorithm was implemented to deconvolute overlapping spectral features in MS2, for better handling spectra with increased spectrum density.&nbsp;Using Orbitrap Astral Zoom MS and Spectronaut® 19.5 software, there was a 12-17% increase in IDs at the precursor level, 10-15% at the peptide level (stripped sequence), and 4-9% at the protein groups level. With Proteome Discoverer software, the gain was 12-19% at the DIA precursor level, 12-17% at the peptide level, and 4-7% at the protein group level. The increase in identifications was primarily observed for low-abundant species. Orbitrap Astral Zoom mass spectrometer also yielded, on average, a 15% higher median number of MS2 data points per peak, while maintaining consistently high quantitation precision and accuracy at the MS2 level.<h4>Conclusions</h4>The novel Orbitrap Astral Zoom mass spectrometer demonstrates improved performance for qualitative and quantitative analysis of complex proteomics samples over a wide range of sample loads spanning four orders of magnitude, from 100 pg to 1µg.<h3>4. Waters Corporation / ASMS: Investigating Fire Fighter and E-waste Handlers Exposure to PFAS Using Liquid Chromatography and Cyclic Ion Mobility Mass Spectrometry</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008883_21620a6293.pdf">Full PDF for download</a></li></ul>PFAS are persistent environmental pollutants linked to health risks, including cancer. Monitoring their levels in human biofluids provides insight into exposure pathways. However, the analysis of PFAS isomers is technically challenging due to their structural similarity. To overcome this, the study employs LC coupled with cyclic ion mobility mass spectrometry (LC-cIM-MS) using the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202">Waters SELECT SERIES Cyclic IMS</a>, which adds a new separation dimension for better specificity.<h4>Methods</h4>Serum samples from Ghanaian firefighters and E-waste workers were prepared using solid-phase extraction (SPE) on 96-well µElution plates. A 22-minute reversed-phase LC gradient was used for separation. The Cyclic IMS system, with ion mobility resolution R~65 and 145, enabled non-targeted PFAS analysis, including the generation of collision cross section (CCS) values to support compound identification.<h4>Results</h4><ul><li>PFAS compounds such as PFOS, PFHxS, PFHpS, PFNA, and 6:2 FTS were identified in real serum samples.</li><li>The cyclic IMS allowed for the separation of coeluting isobaric compounds, such as PFOS isomers and cholic acid biomarkers, minimizing false identifications (Figure 3).</li><li>Figure 4 lists CCS values and other parameters for PFAS standards and identified analytes from a firefighter serum sample.</li><li>Figure 5 shows ion mobility conformeric profiles for PFNA, enabling reliable identification even at low signal intensities.</li></ul><h4>Conclusion</h4>The LC-cIM-MS method:<ul><li>Significantly improves specificity in PFAS identification.</li><li>Enables both targeted and untargeted detection of known and unknown PFAS in complex human samples.</li><li>Can be a key part of future occupational and environmental exposure assessments.</li></ul>

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