High Performance Liquid Chromatography (HPLC) is widely recognized as a premier chemical analytical technique in many industries vital for human existence. Its ability to separate and detect numerous classes of organic and inorganic compounds, as well as determine their concentration in various matrices make HPLC by far the most popular analytical tool in the world.However, its high costs, operational complexity, and the need for highly qualified specialists present significant challenges.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1242"> Alltesta™ Automated Analyzer</a>, simplifies the HPLC analysis process, making it more accessible without compromising quality or accuracy.<h4>Challenge</h4>The laboratory in question faced the challenges of:<ul><li>High costs of HPLC instrumentation and separation columns</li><li>Significant space requirements – traditional HPLC systems take up a large amount of laboratory space, limiting flexibility and accessibility.</li><li>The need for a specialized laboratory environment and employ highly educated and well-trained scientific personnel</li><li>Extensive analytical method development, even for simple analyses.&nbsp;</li></ul>These factors limit the accessibility of HPLC and restrict its use outside specialized laboratories.<h4>Solution: Alltesta™ Automated Analyzer</h4>The laboratory decided to explore SIELC’s offerings and quickly found an Alltesta™ Automated Analyzer that met their requirements. Designed to simplify HPLC workflows and address key user challenges, the system offers:<ul><li>Compact size – Alltesta™ Automated Analyzer features a space-saving design, making it ideal for small laboratories and research environments with limited workspace.</li><li>Ease of use – operates from a standard power outlet and connects to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a> via Wi-Fi, Ethernet, or a mobile network.</li><li>Automated method setup – The company SIELC removes the major burden of analytical method development from an inexperienced system operator, allowing them to focus on obtaining analytical results in minimal time. This is accomplished by SIELC’s experienced staff, who identify the optimal columns, solvents (mobile phases), and gradient parameters for a particular analysis—free of charge for clients. The company also maintains a vast library of analytical methods for numerous compounds and produces a wide variety of HPLC columns to support their implementation.</li><li>Cloud-based control and data processing – server-hosted software enables remote operation and provides analytical results in an easy-to-read format.</li><li>Advanced HPLC technologies – the system features a pulse-free syringe pump (up to 5000 psi) (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Mini Syringe Pump</a>), an autosampler (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta™ Mini-Autosampler</a>), and a multi-wavelength UV detector (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta™ Mini UV-Vis Detector</a>), ensuring reliable analytical performance.</li></ul><figure class="image"><img style="aspect-ratio:1567/972;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg" alt="Bioanalytic: SIELC Alltesta™ Gradient Automated Analyzer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg 245w," sizes="100vw" width="1567" height="972"></figure><h4>Results &amp; Impact&nbsp;</h4>The implementation of Alltesta Automated Analyzer has significantly streamlined the HPLC analysis process. Laboratory report:<ul><li>Reduced costs by minimizing expenses on consumables and cutting method development time.</li><li>Simplified workflow – even individuals without specialized training can perform analyses and obtain accurate results.</li><li>System reliability – extensive testing has shown no hardware or software failures.</li><li>Enhanced data security – HPLC.cloud ensures safe data storage, reliable processing, and secure remote access.&nbsp;</li></ul>Dr. Michael Z. Kagan, PhD, Senior Chromatographer with 28 years of experience at Pfizer, AbbVie, and Coca-Cola, highlights:&nbsp;"During six months of testing, I did not encounter a single hardware or software issue. The system is easy to use, offers flexible column and method configurations, and benefits from expert chromatographic support, making Alltesta a reliable and user-friendly solution."<h4>Conclusion</h4>Alltesta™ Automated Analyzer removes the main barriers associated with HPLC analysis, making it accessible, simple, and efficient. With automation and cloud-based technologies, users can focus on obtaining results rather than dealing with complex instrument settings.

Optimizing HPLC Analysis with Alltesta™ Automated Analyzer

<h3>Summary</h3>High Performance Liquid Chromatography (HPLC) is an essential analytical tool in laboratories across various industries, enabling precise separation and detection of complex compounds. While traditional diode-array HPLC systems are valuable for method development, they can be unnecessary when routine analyses are performed. Our laboratory sought a streamlined solution for standardized separations using a pre-optimized protocol—one that was intuitive, space-efficient, and required minimal hands-on expertise.&nbsp;We adopted the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1242">SIELC Alltesta™ Automated Analyzer</a> compact HPLC system with LED-based detection, which provided an ideal balance of performance, ease of use, and automation. This system significantly improved workflow efficiency, reduced operational demands, and empowered even non-specialist staff to reliably perform high-quality analyses.<h3>Challenge</h3>Our laboratory faced several key constraints that limited the scalability and efficiency of HPLC analyses:<ul><li>Staffing limitations – Traditional HPLC setups often require experienced analytical chemists, creating bottlenecks in routine workflows.</li><li>Space constraints – Larger HPLC systems demand significant bench space, reducing flexibility in smaller lab environments.</li><li>Multitasking difficulties – Monitoring experiments manually detracted from other research activities, limiting productivity.</li></ul>To overcome these challenges, we sought an HPLC system that could provide accurate, repeatable results without requiring specialized personnel or extensive lab resources.<h3>Solution: SIELC’s compact LED-Based Alltesta™ Automated Analyzer</h3>After evaluating available options, our laboratory implemented a compact HPLC system featuring LED detection. This choice allowed us to optimize efficiency while maintaining analytical integrity. The key advantages of this system included:<ul><li>Compact footprint – The small-scale design seamlessly integrated into our lab setup, freeing up valuable workspace.</li><li>User-friendly interface – Intuitive software enabled streamlined control and monitoring, even when multitasking on other unrelated tasks.</li><li>Automated sampling – The high-performance autosampler facilitated unattended operation, reducing manual intervention and improving throughput.</li><li>Simplified detection – LED-based detection was ideal for our needs, providing highly stable and reproducible results for pre-optimized protocols.</li><li>Versatile usability – Interns and non-specialist personnel could operate the system with minimal supervision, lowering staffing requirements.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg" alt="Bioanalytic: Enhancing Routine HPLC Analysis with a Compact LED-Based System: SIELC Alltesta™ Gradient Automated Analyzer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg 245w," sizes="100vw" width="1567" height="972"><h3>Results &amp; Impact</h3>The adoption of the compact LED-based HPLC system led to substantial improvements in laboratory efficiency and accessibility. Key benefits included:<ul><li>Reduced staffing demands – The system’s simplicity enabled non-specialist operators to perform analyses, minimizing reliance on dedicated analytical chemists.</li><li>Increased productivity – Automated sampling and remote monitoring capabilities allowed researchers to focus on other projects while experiments ran in the background.</li><li>Optimized lab space – The system’s compact design enabled seamless integration into our lab, maximizing workspace utility.</li><li>Reliable performance – Routine analyses were executed with high precision and reproducibility, ensuring consistent experimental outcomes.</li></ul><blockquote>Dr. Nikolai Braun, PhD, Research Scientist at Luna Labs, highlights:"Compact, intuitive, and reliable: the Alltesta™ Automated Analyzer has everything you need for routine measurements, revolutionizing what you expect from an HPLC system."</blockquote><h3>Conclusion</h3>By integrating a compact LED-based Alltesta™ Automated Analyzer into our workflow, we successfully enhanced efficiency, reduced operational complexity, and expanded analytical capabilities beyond specialized personnel. This case study highlights the transformative impact of accessible, intuitive chromatography solutions—empowering laboratories to maintain high-quality analyses while minimizing overhead costs and staffing requirements.

Enhancing Routine HPLC Analysis with a Compact LED-Based System

Article

Product

Events

Webinars

Application

Academy

Video

Poster

Scientists

Plastics

Environment

Food

Water

Health

Presentation

Scientific article

Interview

Toxicology

Popularization

Science and research

Software

Covid-19

Career

Laboratories

Various

Zaměření

Type of News

Focus

Bioanalytic started its operations in 1998. The company was born from a passion for science and the need to provide innovative solutions in the laboratory equipment market, from a small business to a dynamically developing company with an established position.

Enhancing Routine HPLC Analysis with a Compact LED-Based System

Optimizing HPLC Analysis with Alltesta™ Automated Analyzer

Upcoming events

Understanding quantification algorithms

36th MassSpec Forum Vienna 2026

Analytical Strategies for Peptide & Protein Therapeutics

Webinars

High-throughput stability screening strategies for antibody formulation

Big Scale, Big Wins: Prep Chromatography Explained

PFAS in Food & Food Contact Material