Learn HPLC column care best practices, including cleaning, regeneration, storage, and preventive maintenance to extend column life, reduce downtime, and ensure reliable chromatographic results.

Learn how two-dimensional liquid chromatography (2D-LC) overcomes the limits of 1D-LC. Explore its principles, operating modes, applications, and the latest advances shaping modern analytical science.

Learn why mobile phase degassing is essential for reliable HPLC performance. Compare inline and offline degassing methods, their advantages, limitations, and practical troubleshooting tips.

Discover how HPLC enables efficient protein purification for biopharmaceuticals, proteomics, and biotechnology. Learn about purification modes, key benefits, optimization strategies, and best practices.

Learn how to safely switch solvents in an LC column, verify solvent miscibility, use IPA between immiscible systems, and prevent irreversible column damage.

This week we bring you application notes by Agilent Technologies, KNAUER, Shimadzu, Thermo Fisher Scientific and Waters Corporation!

A coumarin-based photocleavable solid support allows visible-light release and nonchromatographic purification of RNA oligonucleotides, including UV-sensitive phosphorothioate backbones.

Learn how to balance purity, yield, and throughput when scaling analytical HPLC methods to preparative chromatography.

Learn how silica end-capping improves HPLC column performance. Discover how C18, PS-C18, Polar C18, and XB-C18 chemistries enhance retention, peak shape, and selectivity for various analytes.

Discover practical tips to optimise LC systems for UHPLC performance on any HPLC. Learn how to reduce peak broadening, improve efficiency, and shorten runtimes.