A coumarin-based photocleavable solid support allows visible-light release and nonchromatographic purification of RNA oligonucleotides, including UV-sensitive phosphorothioate backbones.

Learn how to balance purity, yield, and throughput when scaling analytical HPLC methods to preparative chromatography.

Learn how silica end-capping improves HPLC column performance. Discover how C18, PS-C18, Polar C18, and XB-C18 chemistries enhance retention, peak shape, and selectivity for various analytes.

Discover practical tips to optimise LC systems for UHPLC performance on any HPLC. Learn how to reduce peak broadening, improve efficiency, and shorten runtimes.

Column contamination is a leading cause of shortened HPLC column lifetime, leading to high back pressure. Learn why it happens and how to prevent blocked frits with simple precautions.

A UHPLC-MS/MS assay using surrogate calibration and DMIS derivatization quantifies endogenous steroids and contraceptives in plasma with pg/mL sensitivity and regulatory-aligned validation.

C18 columns with residual positive charge improve peak shape of basic analytes at low pH by reducing silanol interactions and enabling 100% aqueous mobile phases.

Struggling with peak tailing of basic compounds in RP-LC using type A silica columns? Discover how low-pH buffers and silanol suppressors can improve peak shape—plus why type B silica is a better choice.

New end-capping technologies can significantly improve the analysis of compounds that were once considered challenging.

How can capacity factors drive method robustness?