<h3 id="h-expedited-screening-improves-outcomes-for-individuals-with-drug-toxicity" data-uw-rm-heading="level" role="heading" aria-level="2">Expedited screening improves outcomes for individuals with drug toxicity</h3>In the field of clinical research and toxicology, the detection and quantification of drugs in human samples with suspected drug overdose or acute drug toxicity are critical. This blog post explores the potential of paper spray mass spectrometry (PS-MS) such as <a target="_blank" rel="noreferrer noopener" aria-label="Thermo Scientific VeriSpray PaperSpray ion source - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html$thermoscientificverispraypapersprayionsource"><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html">Thermo Scientific VeriSpray PaperSpray ion source</a></a>, for faster and more efficient drug toxicity screening in emergency room applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Collaboration_Advances_POC_Solutions_Using_Paper_Spray_Mass_Spec_for_Drug_Tox_Screening_0531bbdb29.jpg" alt="Thermo Fisher Scientific: Collaboration Advances POC Solutions Using PaperSpray Mass Spec for Drug Tox Screening." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Collaboration_Advances_POC_Solutions_Using_Paper_Spray_Mass_Spec_for_Drug_Tox_Screening_0531bbdb29.jpg 234w," sizes="100vw" width="1200" height="800"><article aria-label="Collaboration Advances POC Solutions Using PaperSpray Mass Spec for Drug Tox Screening"><div>Researchers from <a target="_blank" rel="noreferrer noopener" aria-label="King’s College London - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.kcl.ac.uk/forensics$king’scollegelondon"><a target="_blank" rel="noopener noreferrer" href="https://www.kcl.ac.uk/forensics">King’s College London</a></a> (KCL) are collaborating with Guy’s and St Thomas’ NHS Foundation Trust and leveraging the capabilities of paper spray-mass spectrometry (PS-MS) to analyze blood samples from individuals with acute drug toxicity in the emergency room. The aim is to develop routine methods for detecting and quantifying drugs, ultimately improving health outcomes for individuals in need of immediate care and making drug toxicity screening at the bedside a possibility in the future.KCL researchers are conducting extensive testing and optimization of PS-MS technology. Preliminary results shared at The International Association of Forensic Toxicologists (TIAFT) 2023 annual meeting in Rome have shown promising linearity across various concentrations for a number of cathinones. The goal is to refine the process and enhance accuracy, with the potential implementation of PS-MS into clinical practice.<h3 id="h-advantages-of-verispray-paperspray-mass-spectrometry">Advantages of VeriSpray PaperSpray mass spectrometry</h3>PS-MS with the <a target="_blank" rel="noreferrer noopener" aria-label="Thermo Scientific™ TSQ Altis™ Plus Triple Quadrupole - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.thermofisher.com/order/catalog/product/tsq03-10002$thermoscientific™tsqaltis™plustriplequadrupole"><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/404">Thermo Scientific™ TSQ Altis™ Plus Triple Quadrupole</a></a> mass spectrometer offers several advantages over traditional orthogonal techniques. It enables rapid analysis, eliminating the need for lengthy sample preparation and chromatography-based separation. Additionally, the technique provides quantitative or screening results, is easy to use, reduces the risk of cross-contamination, and has barcoded tracking for LIMS compatibility with robotic automated systems such as Hamilton. Plus, it minimizes the use of chemicals and solvents, making it more environmentally friendly.PS-MS offers a fast (2 minutes or less) turnaround time for high-throughput screening, enabling quick analysis of single samples from individuals and identify the presence of drugs or substances. The system’s ease of use and ability to process 240 samples unattended make it efficient and time-saving. The aim is to make drug toxicity screening at the bedside a reality, reducing waiting times for results and improving overall care.<h3 id="h-great-potential-for-poc-toxicity-screening">Great potential for POC toxicity screening</h3>The collaboration between KCL, Guy’s and St Thomas’ NHS Foundation Trust, and research on PS-MS holds great potential for improving point-of-care drug toxicity screening. PS-MS offers a rapid, efficient, and accurate alternative to traditional methods, enabling faster and more efficient care for individuals presenting with acute drug toxicity. By implementing this technology, hospitals can reduce waiting times and ultimately improve outcomes in emergency settings. To learn more, <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/posts/ema-ru%C5%BEi%C4%87-5ba70b133_paper-spray-mass-spectrometry-simplifies-activity-7196531937463750656-0FgR?utm_source=share&amp;utm_medium=member_desktop">download the recent case study</a>.<blockquote><h3 id="h-related-information">Related information</h3><ol><li><a target="_blank" rel="noreferrer noopener" aria-label="Thermofisher.com/verispray - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html$thermofisher.com/verispray"><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html">Thermofisher.com/verispray</a></a></li><li><a target="_blank" rel="noreferrer noopener" aria-label="Toxicology in the emergency department: what’s new? - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://pubmed.ncbi.nlm.nih.gov/36193928/#:~:text=intentional%20and%20accidental%20drug%20overdose,morbidity%20and%20prolonged%20hospital%20admissions.$toxicologyintheemergencydepartment:what’snew?"><a target="_blank" rel="noopener noreferrer" href="https://pubmed.ncbi.nlm.nih.gov/36193928/#:~:text=Intentional%20and%20accidental%20drug%20overdose,morbidity%20and%20prolonged%20hospital%20admissions.">Toxicology in the emergency department: what’s new?</a></a></li><li><a target="_blank" rel="noreferrer noopener" aria-label="Thermo Fisher Scientific collaboration offers potential for faster, more efficient analysis of samples from drug toxicity patients - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.kcl.ac.uk/news/thermo-fisher-scientific-collaboration-offers-potential-for-faster-more-efficient-analysis-of-samples-from-drug-toxicity-patients$thermofisherscientificcollaborationofferspotentialforfaster,moreefficientanalysisofsamplesfromdrugtoxicitypatients"><a target="_blank" rel="noopener noreferrer" href="https://www.kcl.ac.uk/news/thermo-fisher-scientific-collaboration-offers-potential-for-faster-more-efficient-analysis-of-samples-from-drug-toxicity-patients">Thermo Fisher Scientific collaboration offers potential for faster, more efficient analysis of samples from drug toxicity patients</a></a></li><li><a target="_blank" rel="noreferrer noopener" aria-label="Paper spray mass spectrometry simplifies and expedites turnaround of drug toxicity screening: Thermo Fisher Scientific Case Study - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://view.highspot.com/viewer/6644c7d165ad350931b0517c$paperspraymassspectrometrysimplifiesandexpeditesturnaroundofdrugtoxicityscreening:thermofisherscientificcasestudy"><a target="_blank" rel="noopener noreferrer" href="https://view.highspot.com/viewer/6644c7d165ad350931b0517c">Paper spray mass spectrometry simplifies and expedites turnaround of drug toxicity screening: Thermo Fisher Scientific Case Study</a></a></li></ol></blockquote></div></article><section><blockquote><h4>Courtney Patterson</h4><div itemprop="description">Courtney Patterson is an Application Scientist at Thermo Fisher Scientific, specializing in the development of LC-MS applications for forensic and clinical toxicology using targeted and untargeted approaches to quantify and confirm drugs of abuse.</div></blockquote></section>

Collaboration Advances POC Solutions Using PaperSpray Mass Spec for Drug Tox Screening

Hydrophilic interaction liquid chromatography: An efficient tool for assessing thorium interaction with phosphorylated biomimetic peptides

Introduced nearly 80 years ago, collision-based ion-activation and dissociation is the most commonly used type of fragmentation commercially available today. In principle, this technique is quite straightforward: apply energy to accelerate the particles of interest until they collide with an inert gas to induce fragmentation. Within these parameters, there are many different sub-categories of the technique. Here we will discuss three collision-based techniques that occur on Thermo Scientific mass spectrometers.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Freedom_to_Fragment_Introduction_The_importance_of_fragmentation_in_mass_spectrometry_1_c8f70d1281.jpg" alt="Thermo Fisher Scientific Freedom to Fragment Introduction" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Freedom_to_Fragment_Introduction_The_importance_of_fragmentation_in_mass_spectrometry_1_c8f70d1281.jpg 245w," sizes="100vw" width="1024" height="512"><h3>Higher-Energy Collisional Dissociation (HCD)</h3><ol><li>Process: HCD is a beam-based technique that occurs in the HCD cell or ion routing multipole, where the ion beam is directed into a potential well. Excited molecules collide with collision gas, causing fragmentation. This gas is typically nitrogen and is fast and efficient, but indiscriminate in technique. To ensure a higher energy collision, the potential energy is dropped (typically from the quadrupole into the collision cell) by many 10s of eV. This ensures the ions have high energy as they enter the cell with nitrogen and collide with enough energy for the fragmentation to occur. In peptides and proteins, HCD favors b- and y-ion formation. [Figure 1]</li><li>Applications: HCD is commonly used for general proteomics and metabolomics.</li><li>Considerations: When using Thermo Scientific mass spectrometers for HCD fragmentation, we generally use normalized collisional energy (NCE) instead of directly putting in the eV of potential well “drop”. The calibration routine determines the optimal voltage for a series of ions in the calibration solution.&nbsp; Then the routine scales these absolute voltages to a “normalized collision energy” value of 30. As such, an NCE value of 30 should work well across a range of m/z values and charge states. To see the actual applied value, one can check the scan header of the spectrum easily in our Thermo Scientific Freestyle software.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Collision_Based_Ion_activation_and_Dissociation_92107a9524.jpg" alt="Thermo Fisher Scientific: Collision-Based Ion-activation and Dissociation: Figure 1: Location of peptide/protein backbone cleavage and its nomenclature. Representative here is a 4-amino acid peptide with side chains labeled R1-4." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Collision_Based_Ion_activation_and_Dissociation_92107a9524.jpg 245w," sizes="100vw" width="1200" height="582"><h3>Collision-Induced Dissociation (CID) (ion trap specific version)</h3><ol><li>Process: CID, as it occurs in Thermo Fisher Scientific’s dual linear ion trap technology, is a resonance-based technique where molecules are excited by a specific frequency.&nbsp; These excited molecules will start running into He molecules at a faster rate.&nbsp; These collisions slowly heat the resonant molecules until they fragment. Once fragmentation occurs, the product ions at new m/z values are no longer excited by the frequency and will cool back down to the center of the trap. In peptides and proteins, CID favors b- and y-ion formation.</li><li>Applications: CID is great for small molecules and peptides and helps fragment oligonucleotides, especially when the q-value is adjusted.&nbsp; It also fragments with higher efficiency, which makes it well-suited for MS3 and higher workflows.</li><li>Considerations: Because CID is resonance-based, there is a compromise outlined in the Matthieu equation. With certain voltages and frequencies applied, only certain m/z values can be trapped. Thus, we have something called the 1/3rd rule. This rule approximates the equation and states that any fragment created that is &lt; 1/3 the m/z of the precursor will not be trapped. This rule makes CID less desirable as a primary fragmentation technique, thus more used as the pinch hitter when needed for alternate fragmentation after HCD is shown inefficient for a specific molecule. Furthermore, CID is a slow heating process.&nbsp;As such, activation/fragmentation takes a few milliseconds, whereas HCD is effectively instantaneous.&nbsp;</li></ol>Recent developments in oligonucleotide analysis have shown great strides in using CID for fragmentation of many types, including tRNA, snRNA and digested mRNA. To gain the most impact from these experiments, scientists can adjust the q-value applied within the CID resonance. This is usually set to 0.25; however, for oligonucleotides, lowering that value to 0.2-0.15 can help prevent unwanted fragmentation from occurring and specify backbone cleavage that can be more easily characterized.<h3>Source Induced Dissociation (SID)</h3><ul><li>Process: SID is a collisional-induced activation (and if desired, dissociation) technique that is induced in the source region of mass spectrometers. By applying more voltage in the source region, ions are drawn into the mass spectrometer with more energy. Within the source region, there are still atmospheric inert gases that have been drawn in with the analytes of interest. With the additional energy, collisions with these inert gases can occur. Since SID occurs before any mass analyzer, it applies to all ions that enter the mass spectrometer.</li><li>Applications: SID is most commonly used as a desolvation technique for large native molecules. By applying SID energy in the source, molecules generate vibrational energy, and non-covalent, low-energy bonds will be broken only.</li><li>Analogy: SID could be compared to shaking off an umbrella after coming in from the rain. The jostle doesn’t cause the whole umbrella to fall apart, but it does cause the water droplets to fall off. This is a little like proteins getting desolvated from SID.</li><li>Considerations: SID can be a destructive, dissociative process as well, so if that is not the goal, it is important to monitor the energy applied to the analyte. For example, it has been common practice in top-down proteomics to use a small (10-15 eV) setting for SID to aid in desolvation. However, I have successfully taken pure protein (or well chromatographically separated protein) and applied much higher SID (&gt;50 V) to induce dissociation. The resulting fragmentation pattern can yield surprisingly good results. However, this is not common practice, especially not for coeluting species, as the fragmentation would occur on all proteins eluting.</li></ul>Another consideration for SID is its use in native MS. This is often vital for good detection of native proteins like antibodies. Often, we will employ a stronger SID in these cases, anywhere from 60-120 V, depending on the size and complexity of the protein/protein complex, to ensure proper desolvation and thus detection.<blockquote>Learn more about <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/fragmentation-solutions.html">collisional induced dissociation (CID) and higher energy collisional induced dissociation (HCD)</a>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#CID #HCD #fragmentation</blockquote><section><blockquote><h4>Amanda Lee</h4>Amanda Lee PhD, Product Marketing Manager</blockquote></section>

Collision-Based Ion-activation and Dissociation

<h3>Smarter sample preparation for LC-MS: from simplicity to selectivity</h3>In any toxicology lab, sample preparation is more than a preliminary step—it’s the foundation for accuracy, reproducibility, and workflow efficiency. In a recent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3978">webinar</a>, we explored a range of sample preparation strategies—from minimal processing to highly selective techniques—with additional insights shared by Dr. William Clarke, PhD, MBA, DABCC, Professor of Pathology and Deputy Director of Quality and Regulatory Affairs at Johns Hopkins University School of Medicine. These discussions build on our latest white paper, “<a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-000861-lsms-clinical-tox-explorer-toxicology-wp000861-na-en.pdf">Context matters: selecting LC-MS sample preparation methods for LC-MS/MS in clinical research and toxicology applications</a>“, authored by Prof. Alain Verstraete (University Hospital Ghent). The paper highlights commonly used LC-MS sample preparation methods and provides practical guidance to help laboratories select the most appropriate approach for their needs.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_5_722a357221.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Thermo Scientific SOLAμ SPE Plates—Explore SOLA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_5_722a357221.jpg 245w," sizes="100vw" width="700" height="340"><h3>The power of simplicity: dilute-and-shoot and protein precipitation</h3>For relatively clean matrices like urine, a dilute-and-shoot approach can be effective. This method involves minimal handling and is low-cost but generally best suited for low-protein samples to avoid ion suppression or instrument issues. For samples rich in proteins, such as plasma, serum, or whole blood, protein precipitation (PPT) is a common and straightforward method. It uses organic solvents—often methanol—to denature and precipitate proteins. After incubation at low temperatures and centrifugation, the supernatant is collected and may be further diluted. Additives like zinc sulfate can be added to enhance protein precipitation or aid cell lysis. While PPT is less selective, it effectively reduces matrix complexity and is compatible with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">robust LC-MS instrumentation</a>. As a plug-and-play approach, the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">Thermo Scientific Tox Explorer</a> method, with over 2,000 compounds in its library, includes sample preparation recommendations for varying matrices, including dilute-and-shoot and protein precipitation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_4_658266d072.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Tox Explorer compound library shown in mzVault." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_4_658266d072.jpg 245w," sizes="100vw" width="1024" height="480">Enhancing selectivity: Phospholipid Removal, Liquid-Liquid Extraction (LLE), Supported Liquid Extraction (SLE), and Solid-Phase Extraction (SPE)When higher selectivity and sensitivity are required, extraction-based methods are employed:<ul><li>Phospholipid Removal uses commercial solutions to reduce matrix components that can cause ion suppression. Though relatively simple, it requires some specialized materials and does not concentrate analytes.</li><li>Liquid-Liquid Extraction (LLE) separates analytes based on their solubility differences between aqueous biological fluids and immiscible organic solvents. This technique removes many matrix interferences but is labor-intensive and challenging to automate.</li><li>Supported Liquid Extraction (SLE)&nbsp;improves upon LLE by using a porous support to retain the aqueous phase, allowing nonpolar analytes to partition into an organic solvent, which can be collected more efficiently as flow-through. SLE offers cleaner extracts and better reproducibility, making it more compatible with higher-throughput workflows.</li><li>Solid-Phase Extraction (SPE) selectively retains analytes on sorbent phases based on chemical characteristics such as polarity or charge. Impurities are washed away, and analytes are eluted with strong organic solvents, often followed by drying and reconstitution. SPE improves consistency and reduces matrix effects, especially in complex samples or high-throughput environments. Various sorbents, including ion exchange types, can be selected, or even combined, depending on analyte chemistry.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_2_fee3c7bdd8.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Common sample preparation methods for LC-MS featured in table 1 of white paper 000861." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_2_fee3c7bdd8.jpg 245w," sizes="100vw" width="1000" height="548"><h3>Unlocking efficiency: how automation is transforming sample preparation</h3>As labs face staffing shortages and increasing sample volumes, automation is becoming essential. Solutions from providers like Hamilton and Tecan help streamline sample preparation tasks, including pipetting, plate transfers, and offline SPE. This can dramatically reduce hands-on time and standardize processes. In the webinar, Dr. Clarke shares that one lab, for example, was able to cut total sample preparation time in half—from six hours to three—and reduce hands-on analyst time from three hours to just 10 minutes by using a Tecan platform with SPE plates. Each of the extraction methods discussed can be readily programmed onto these liquid handling robots. Still, automation brings new challenges—like programming robots, accounting for sample variability, managing liquid classes (e.g., whole blood vs. serum), and replicating the precision of skilled human pipetting, which remains difficult to fully automate and underscores the continued importance of human expertise<h3>Emerging trends: dried blood spots and microsampling</h3>Dried blood spots (DBS) and microsampling techniques promise minimally invasive collection and improved sample logistics. Fixed-volume devices with or without pre-punched filter cards can simplify automation and downstream analysis. However, challenges remain: hematocrit effects, uneven sample spread, storage issues, and potential for contamination or hemolysis.Capillary sampling from fingersticks may seem like a step toward decentralized testing, but it’s not without challenges—sample quality can be affected by peripheral blood differences or inconsistent collection techniques.To address some of these limitations, particularly the need for extensive sample prep for screening purposes, direct analysis methods are gaining traction. The <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html">Thermo Scientific VeriSpray PaperSpray ion source</a> offers a direct MS-based ionization approach for analyzing dried samples, eliminating the need for time-consuming sample preparation and chromatographic separation. This can be especially useful for complex matrices like whole blood, which often require more intensive extraction to remove proteins.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_3_cb6f7e0282.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: VeriSpray paper spray ionization is a rapid and user-friendly sample introduction technique that delivers samples directly to the mass spectrometer using electrospray ionization. The process is straightforward—just add the sample and solvent to the paper." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_3_cb6f7e0282.jpg 245w," sizes="100vw" width="1024" height="271"><h3>Final takeaway: clean samples, better results</h3>The overarching message: cleaner samples drive better assay performance. Whether you’re processing high-throughput toxicology screens or quantifying small molecules and peptides, aligning your sample preparation strategy with both analytical and operational goals is essential. From simple dilute-and-shoot methods to advanced approaches or fully automated workflows, thoughtful preparation can enhance instrument uptime, improve assay accuracy, and reduce long-term operational costs. A recent technical note—linked under the SPE section in the supporting documents below—demonstrates this effectively using a large urine drug panel. Solid-phase extraction plates improved both assay robustness and accuracy. By extracting just 200 µL of sample and minimizing solvent use, the workflow successfully reduced reagent costs and waste without sacrificing data quality. As mass spectrometry continues to expand into more complex and decentralized applications, laboratories that invest in fit-for-purpose sample prep strategies will be best positioned to deliver confident, high-quality results at scale. To support these efforts, Thermo Fisher Scientific offers a variety of aligned resources, including those discussed in the webinar and this blog:<ul><li>Dilute-and-shoot: See the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_000794_lsms_tox_explorer_orbitrap_exploris_120_drugs_of_abuse_tn000794_na_en_3286411f97/tn-000794-lsms-tox-explorer-orbitrap-exploris-120-drugs-of-abuse-tn000794-na-en.pdf">Thermo Scientific Tox Explorer method for Orbitrap-based screening of 120 drugs of abuse</a> and <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-000861-lsms-clinical-tox-explorer-toxicology-wp000861-na-en.pdf">this white paper highlighting the comparison of sample prep methods.</a></li><li>Protein Precipitation: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73168_lc_ms_drugs_of_abuse_tn73168_en_6ff028434e/tn-73168-lc-ms-drugs-of-abuse-tn73168-en.pdf">Quantification of 78 drugs of abuse in human blood by liquid chromatography – HRAM Orbitrap mass spectrometry for clinical research</a>.</li><li>Solid phase extraction (SPE): Learn more through our tox drugs of abuse <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-002363-clinical-lc-ms-homestead-tn002363-na-en.pdf">Thermo Scientific Orbitrap Exploris 120 MS</a> and <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-002606-lsms-clinical-toxicology-doa-tn002606-na-en.pdf">Thermo Scientific TSQ Quantis Plus MS method for clinical toxicology</a> technical notes.</li><li>Automation: See how oral fluid extraction is streamlined using automation in <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-003851-cl-lcms-stellar-oral-fluid-tn003851-na-en.pdf">this technical note featuring Thermo Scientific Stellar MS.</a></li><li>Dried sample workflows: Explore simplified prep using the Thermo Scientific VeriSpray PaperSpray Ion Source in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73467_ps_ms_paperspray_tandem_ms_drugs_of_abuse_urine_tn73467_en_d81464d205/tn-73467-ps-ms-paperspray-tandem-ms-drugs-of-abuse-urine-tn73467-en.pdf">this technical note on urine drug analysis.</a></li></ul><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#SamplePreparation, #ForensicToxicology, #toxicology, #lcms</blockquote><blockquote><h4>Ema Ruzic</h4>Vertical Marketing Manager, Chromatography and Mass Spectrometry Division (CMD)</blockquote>

Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow

In a recent study published in the Journal of Analytical Toxicology, researchers from Sheffield Teaching Hospitals NHS Foundation Trust validated the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">Thermo Scientific Tox Explorer method for screening and quantifying drugs and their metabolites</a> in post-mortem vitreous humor. This marks their second publication demonstrating the versatility of this all-in-one LC-MS/MS solution for forensic toxicology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Beyond_Blood_Sheffield_Teaching_Hospitals_NHS_Foundation_Trust_Develops_HRAM_Powered_Method_for_Post_Mortem_Toxicology_Analysis_3347a9e966.jpg" alt="Thermo Fisher Scientific: Beyond Blood: Sheffield Teaching Hospitals NHS Foundation Trust Develops HRAM-Powered Method for Post-Mortem Toxicology Analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Beyond_Blood_Sheffield_Teaching_Hospitals_NHS_Foundation_Trust_Develops_HRAM_Powered_Method_for_Post_Mortem_Toxicology_Analysis_3347a9e966.jpg 245w," sizes="100vw" width="1434" height="791"><h3>Why analyze vitreous humor?</h3>Vitreous humor, the gel-like fluid within the eye, offers several forensic advantages. It’s less prone to post-mortem redistribution, contamination, and decomposition than blood, and often exhibits superior chemical stability. However, its use has traditionally been limited due to small sample volumes and the need for highly sensitive analytical methods.Dr. Edmund Rab, Consultant Clinical Scientist and Clinical Lead for Clinical Chemistry, Sheffield Teaching Hospitals NHS South Yorkshire and Bassetlaw Pathology, explains:“Post-mortem redistribution is a perennial problem for the interpretation of post-mortem toxicology results. The measurement of drugs and their metabolites in vitreous humour can help identify whether a high blood analyte concentration is due to excessive use or post-mortem redistribution. This information can be vital when determining how an individual has come about their death.”To address this, Dr. Rab’s team investigated whether their previously validated blood and urine Tox Explorer method could reliably screen for and quantify compounds in vitreous humor. Their results proved it could.<h3>Method highlights and key findings</h3>Using the Thermo Scientific Tox Explorer HRAM library, the Sheffield team achieved:<ul><li>Detection and quantitation of 48 compounds with bias and precision within ±20% across all tested concentrations with LOQs as low as 0.2 ng/mL and ULOLs up to 219 µg/mL.</li><li>Low detection limits, down to 0.0008 mg/L, with minimal ion suppression or carryover.</li><li>Higher detectability of unstable compounds like 6-monoacetylmorphine, codeine, olanzapine, and cocaine in vitreous humor compared to blood.</li><li>Validated dilution integrity and analyte stability, confirming the workflow’s robustness with low-volume samples and sample stability for at least 4 days at 10 degrees Celsius.</li></ul><h5>Dr. Rab further notes:</h5>“The Tox Explorer workflow is an excellent resource for laboratories performing toxicological analysis. Having an extensive library available for use saves the user the time and expense of developing their own library, but at the same time the library can be easily customised to include the compounds that suit a specific application.”<h3>Insights from real-world cases</h3>The researchers analyzed 129 post-mortem cases. In most, drug profiles in vitreous humor aligned with those found in blood or urine. In some cases, drugs were detectable in vitreous humor even when not present in blood—highlighting its value when traditional matrices are degraded or unavailable.<h3>Tox Explorer: empowering modern forensic workflows</h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/xx_73747_lc_ms_tox_explorer_solution_guide_xx73747_en_c893037858.pdf">The Tox Explorer Collection</a> is purpose-built to support comprehensive toxicology workflows with unmatched flexibility and ease of use. In addition to including a wide variety of drugs of abuse, including stimulants, opioids, cannabinoids, and benzodiazepines, the Tox Explorer Collection also provides retention times, SRM libraries, and a high-resolution library. It supports both:<ul><li>High-resolution accurate mass (HRAM) platforms such as the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3&amp;search=exploris+">Thermo Scientific Orbitrap MS</a></li><li>Triple quadrupole systems like the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=tsq">Thermo Scientific TSQ Plus Mass Spectrometer</a></li></ul>Both configurations offer confident screening, confirmation, and quantitation within a single workflow.Advanced HRAM capabilities include:<ul><li>Retrospective data analysis—ideal for emerging substances</li><li>Simultaneous positive/negative ion detection in a single run</li><li>Comprehensive MS/MS spectral acquisition without averaging</li><li>Exceptional mass accuracy, even at low m/z values over a 15-day period</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Beyond_Blood_Sheffield_Teaching_Hospitals_NHS_Foundation_Trust_Develops_HRAM_Powered_Method_for_Post_Mortem_Toxicology_Analysis_2_719c11d20e.jpg" alt="Thermo Fisher Scientific: Beyond Blood: Sheffield Teaching Hospitals NHS Foundation Trust Develops HRAM-Powered Method for Post-Mortem Toxicology Analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Beyond_Blood_Sheffield_Teaching_Hospitals_NHS_Foundation_Trust_Develops_HRAM_Powered_Method_for_Post_Mortem_Toxicology_Analysis_2_719c11d20e.jpg 245w," sizes="100vw" width="700" height="410">With built-in retention time libraries, standardized methods, user-friendly software, and onsite applications training, Tox Explorer provides a streamlined, scalable solution from sample preparation through reporting.<h3>A platform built for progress</h3>This latest validation from Sheffield confirms vitreous humor as a viable and valuable matrix for forensic toxicology—and underscores how Tox Explorer continues to expand analytical possibilities across the field. From post-mortem casework to clinical research and forensic labs, Tox Explorer empowers scientists to achieve greater sensitivity, reliability, and confidence in every analysis. Discover more at <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">Tox Explorer Collection for LC-MS</a> and read the full article <a target="_blank" rel="noopener noreferrer" href="https://academic.oup.com/jat/advance-article/doi/10.1093/jat/bkaf049/8155852?utm_source=authortollfreelink&amp;utm_campaign=jat&amp;utm_medium=email&amp;guestAccessKey=d81359d6-27c5-4162-86a8-c0d84937088b">Simultaneous screening and quantitation of drugs and their metabolites in post-mortem vitreous humor by liquid chromatography–high resolution mass spectrometry</a>.<h3>Explore more resources and earlier posts in this blog series:</h3><ol><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6189">Part 1 of our toxicology series on Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow</a></li><li>Technical notes by specimen type</li></ol><h4>Urine</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_002363_clinical_lc_ms_homestead_tn002363_na_en_479dc65907.pdf">Utilizing HRAM Orbitrap mass spectrometry to quantitate 106 drugs of abuse in urine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_002606_lsms_clinical_toxicology_doa_tn002606_na_en_c49fd49d9f.pdf">Utilizing triple quadrupole mass spectrometry to quantitate 106 drugs of abuse in urine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_66050_lc_ms_orbitrap_exploris_fentanyl_analog_compounds_tn66050_en_4fff13e3fd/tn-66050-lc-ms-orbitrap-exploris-fentanyl-analog-compounds-tn66050-en.pdf">Screening and Semi-Quantitation of 212 Fentanyl Analog Compounds by the Orbitrap Exploris 120 Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_001734_quantitation_22_fentanyl_analog_compounds_tsq_altis_md_mass_spectrometer_tn001734_en_2810192422.pdf">Quantitation of 22 fentanyl analog compounds with a TSQ Altis MD mass spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_66004_lc_hram_ethyl_glucuronide_sulfate_human_urine_tn66004_en_59380bae9b.pdf">Quantification of Ethyl Glucuronide and Ethyl Sulfate in Human Urine by LC-HRAM(MS) for Clinical Research</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TN_64970_LC_MS_TSQ_Quantis_Et_G_Et_S_Urine_TN_64970_EN_9129c15cc0/TN-64970-LC-MS-TSQ-Quantis-EtG-EtS-Urine-TN64970-EN.pdf">Analysis of ethyl glucuronide and ethyl sulfate in urine with TSQ Quantis MS</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_65182_lc_ms_drugs_abuse_urine_tn65182_en_22dbefa84c/tn-65182-lc-ms-drugs-abuse-urine-tn65182-en.pdf">Tomorrow’s quantitation with LC-MS/MS: fast screening and quantitation of drugs of abuse in urine for forensic toxicology</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73467_ps_ms_paperspray_tandem_ms_drugs_of_abuse_urine_tn73467_en_d81464d205/tn-73467-ps-ms-paperspray-tandem-ms-drugs-of-abuse-urine-tn73467-en.pdf">Quantitative Analysis of Drugs of Abuse and their Metabolites in Urine using PaperSpray Tandem Mass Spectrometry</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_000553_lsms_paperspray_urine_oral_fluid_cannabinoids_tn000553_na_en_10f4386549.pdf">Quantitative analysis of cannabinoids in urine and oral fluid using reactive paper spray tandem mass spectrometry for clinical research and forensic toxicology</a></li></ul><h4>Whole blood</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73168_lc_ms_drugs_of_abuse_tn73168_en_6ff028434e/tn-73168-lc-ms-drugs-of-abuse-tn73168-en.pdf">Quantification of 78 drugs of abuse in human blood by liquid chromatography – HRAM Orbitrap mass spectrometry for clinical research</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73360_lc_ms_drugs_of_abuse_human_blood_tn73360_en_878e3ac14b/tn-73360-lc-ms-drugs-of-abuse-human-blood-tn73360-en.pdf">Quantification of drugs of abuse in human blood by TurboFlow chromatography coupled to tandem mass spectrometry for use in clinical research</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_65420_paperspray_ms_controlled_substances_tn65420_en_10a63ca0be/tn-65420-paperspray-ms-controlled-substances-tn65420-en.pdf">Detection of controlled substances in blood samples using the VeriSpray ion source with TSQ Altis MS for clinical research and forensic toxicology</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73307_paperspray_faims_immunos_blood_tn73307_en_02d22e002c/tn-73307-paperspray-faims-immunos-blood-tn73307-en.pdf">Combined PaperSpray and FAIMS Technology for Rapid Quantification of Immunosuppressants in Whole Blood</a></li></ul><h4>Serum/plasma</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_66051_lc_hram_ms_quantitation_34_benzodiazepines_human_plasma_tn66051_en_f9bdfdb0fe/tn-66051-lc-hram-ms-quantitation-34-benzodiazepines-human-plasma-tn66051-en.pdf">Quantification of 34 Benzodiazepines in Human Plasma by LC-HRAM-MS for Clinical Research</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_66052_lc_ms_tricyclic_antidepressants_human_plasma_tn66052_en_59fa9a02d3/tn-66052-lc-ms-tricyclic-antidepressants-human-plasma-tn66052-en.pdf">Quantification of 15 tricyclic antidepressants in human plasma by liquid chromatography- tandem mass spectrometry for clinical research</a></li></ul><h4>Oral fluid</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_000553_lsms_paperspray_urine_oral_fluid_cannabinoids_tn000553_na_en_10f4386549.pdf">Quantitative analysis of cannabinoids in urine and oral fluid using reactive paper spray tandem mass spectrometry for clinical research and forensic toxicology</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_003851_cl_lcms_stellar_oral_fluid_tn003851_na_en_e8f9325297.pdf">Quantitation of an oral fluid drug panel including THC using the new Stellar mass spectrometer</a></li></ul><blockquote><ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#ForensicToxicology #HRAM #ToxExplorer #LCMS</li></ul><h4>Ema Ruzic</h4>Vertical Marketing Manager, Chromatography and Mass Spectrometry Division (CMD)</blockquote>

Beyond Blood: Sheffield Teaching Hospitals NHS Foundation Trust Develops HRAM-Powered Method for Post-Mortem Toxicology Analysis

News from LabRulezLCMS Library - Week 02, 2026

A critical first step in quantitation is tuning the instrument to identify the optimal fragment ions that deliver both sensitivity and specificity. This is true regardless of the use of triple quadrupoles, ion traps, or accurate mass systems.&nbsp;Traditionally, this process requires physical standards and, in the case of large panels of analytes, can be time-consuming.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Generating_Quantitative_Methods_Automatically_From_Spectral_Library_Data_262243e285.jpg" alt="Thermo Fisher Scientific: Generating Quantitative Methods Automatically From Spectral Library Data" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Generating_Quantitative_Methods_Automatically_From_Spectral_Library_Data_262243e285.jpg 245w," sizes="100vw" width="1024" height="512"><h3>But what if we could generate methods without needing all the standards on hand or even tuning them at all?&nbsp;</h3>Doing so would require having a source of data that captures the optimal fragments and energies for all compounds.&nbsp;Enter <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, a spectral library that includes up to 20 collision energies for high-energy collisional dissociation MS2 on every compound in the library.Although generated from full scan accurate mass instruments, this information contains the energy-dependent fragment formation using a fragment technique common to triple quadrupoles and other instruments.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_8_9199b4056a.jpg" alt="Thermo Fisher Scientific: Generating Quantitative Methods Automatically From Spectral Library Data: Thermo Scientific mzCloud Mass Spectral Library" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_8_9199b4056a.jpg 224w," sizes="100vw" width="270" height="188"><ul><li>Extensively curated, high-quality free online library</li><li>Multiple fragmentation energy levels represented</li><li>Covers a wide chemical space to include compounds relevant to all applications</li><li>For both research and targeted screening workflows</li><li>Contains over 32,000 compounds and 16,500,000 spectra</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Generating_Quantitative_Methods_Automatically_From_Spectral_Library_Data_2_bcac20a85c.jpg" alt="Thermo Fisher Scientific: Generating Quantitative Methods Automatically From Spectral Library Data: Figure 1. Breakdown curves for the top 6 fragment ions from Melatonin showing maximum energy for each fragment ion. X-axis is fragmentation energy, Y-axis is fragment intensity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Generating_Quantitative_Methods_Automatically_From_Spectral_Library_Data_2_bcac20a85c.jpg 245w," sizes="100vw" width="1024" height="367"><h3>So, could we transfer the information from the library into a usable method for an instrument such as a triple quadrupole?</h3>The answer is yes. The first step is to determine if any offset in the collision energy (eV) exists between the platform used to generate the library and the target quantitative instrument.&nbsp; This can be easily assessed with a small selection of compounds across the mass range to ensure that the optimal collision energy remains the same or, in cases where they are different, a correction factor can be calculated.&nbsp; Once this is done, it is a relatively simple matter of taking the top N most intense fragment ions from the library curves and creating the corresponding quantitative instrument methods.In fact, we have already successfully implemented this approach: all recent triple quadrupoles from Thermo Fisher Scientific now come with a built-in database of methods generated in this fashion.&nbsp; The creation of a quantitative acquisition method is as simple as selecting compounds, polarity, and number of transitions from the database and proceeding with acquisition.By automating the generation of quantitative methods from spectral library data, we significantly reduce the time and effort required for method development. This enables our customers to focus on their core research and analysis, improving productivity and accelerating scientific discovery.For more information, join us at the upcoming <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/events/proteome-discoverer-compound-discoverer-users-meetings.html">user meeting</a> or go to <a target="_blank" rel="noopener noreferrer" href="https://www.mzcloud.org/">mzCloud.org</a> or see the advantages of using mzCloud with Thermo Scientific <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer software</a>.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#mzCloud #MassSpectralLibraries</li></ul>

Generating Quantitative Methods Automatically From Spectral Library Data

Software

Article

Product

Events

Webinars

Application

Academy

Video

Poster

Scientists

Plastics

Environment

Food

Water

Health

Presentation

Scientific article

Interview

Toxicology

Popularization

Science and research

Covid-19

Career

Laboratories

Various

Zaměření

Type of News

Focus

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

Generating Quantitative Methods Automatically From Spectral Library Data

News from LabRulezLCMS Library - Week 02, 2026

Beyond Blood: Sheffield Teaching Hospitals NHS Foundation Trust Develops HRAM-Powered Method for Post-Mortem Toxicology Analysis

Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow

Collision-Based Ion-activation and Dissociation

Hydrophilic interaction liquid chromatography: An efficient tool for assessing thorium interaction with phosphorylated biomimetic peptides

Collaboration Advances POC Solutions Using PaperSpray Mass Spec for Drug Tox Screening

Upcoming events

Understanding quantification algorithms

36th MassSpec Forum Vienna 2026

Analytical Strategies for Peptide & Protein Therapeutics

Webinars

High-throughput stability screening strategies for antibody formulation

Big Scale, Big Wins: Prep Chromatography Explained

PFAS in Food & Food Contact Material