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Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology

 

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DEVELOPMENT OF A BIOANALYTICAL SPE LC-MS/MS ASSAY FOR OLIGONUCLEOTIDES Mary Lame, Kathryn Brennan, Michael Donegan, Thanai Paxton, and Ian Edwards Waters Corporation, 34 Maple Street, Milford, MA , USA INTRODUCTION RESULTS Over the past decade, there has been a dramatic…
Key words
ost, ostmassprep, masspreparea, areaspe, speoasis, oasisoligonucleotide, oligonucleotidetea, tearecovery, recoverywax, waxtime, timehlb, hlbtic, ticoligonucleotides, oligonucleotidesbioanalytical, bioanalyticalwaters
Improving LC-MS/MS Bioanalytical Quantitation of Oligonucleotides using New High Performance Tandem Quadrupole Mass Spectrometer Suma Veeramachineni1; Suraj Dhungana1; Ying-Qing Yu1; Mark Wrona1; 1Waters Corporation, Milford, MA Introduction Results Improving LC-MS/MS bioanalytical performance especially for ESI negative analytes has been of…
Key words
oligonucleotide, oligonucleotideaccuracy, accuracyoligonucleotides, oligonucleotidesosts, ostsperformance, performanceantisense, antisensequantitation, quantitationbioanalytical, bioanalyticallloq, lloqpremier, premierpolythymidine, polythymidinestd, stdonts, ontsconc, concwaters
Application Note LC-MS Bioanalytical Quantification of a GalNAc-siRNA Conjugate Oligonucleotide Using Semi-Automated Solid Phase Extraction Mary Trudeau, Kathryn Brennan Waters Corporation Abstract The following work demonstrates the capabilities of the Pipette+ in combination with the Otto SPEcialist for semi-automated liquid…
Key words
galnac, galnacsirna, sirnaconjugate, conjugateoligonucleotide, oligonucleotidesemi, semibioanalytical, bioanalyticalautomated, automatedquantification, quantificationotto, ottoextraction, extractionsolid, solidspecialist, specialistphase, phaseusing, usingpipette
Application Note Sensitive LC-MS/MS Bioanalytical Quantitation of Antisense Oligonucleotides Suma Veeramachineni, Mark D. Wrona Waters Corporation Abstract This application demonstrates the quantitative and qualitative capabilities of the Waters™ Xevo™ TQ Absolute Tandem Quadrupole Mass Spectrometer (MS/MS) and its suitability for…
Key words
antisense, antisenseoligonucleotides, oligonucleotidesbioanalytical, bioanalyticalsensitive, sensitivequantitation, quantitationpremier, premiertandem, tandemacquity, acquityabsolute, absolutewaters, watershps, hpsmaxpeak, maxpeakoligonucleotide, oligonucleotidehuman, humansurfaces
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