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Application NoteImproved Oligonucleotide SPE-LC-MSAnalysis Using MaxPeak High PerformanceTechnologyKathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. RainvilleWaters Corporation AbstractThe demand for highly selective and sensitive LC-MS bioanalytical assays in support of researchand development of next-generation oligonucleotide therapies has greatly increased. The...
Key words
oligonucleotide, oligonucleotideacquity, acquityspe, spexevo, xevomaxpeak, maxpeakpremier, premieryielded, yieldeduplc, uplcbioanalytical, bioanalyticaltargetlynx, targetlynxtea, teaassays, assaysquantification, quantificationrecovery, recoverydemand
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Developing a Novel, Integrated LC-MS Workflow for High-resolutionMonitoring and Characterization of OligonucleotidesHenry Shion, Robert Birdsall, and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Optimized RP-UPLC mobile phaseMethods that enable structural characterization, molecular weightcompositions for better oligonucleotideconfirmation, and impurity analysis and...
Key words
promass, promassoligonucleotide, oligonucleotideost, ostoligonucleotides, oligonucleotidesuplc, uplcpolyt, polytacquity, acquitymasslynx, masslynxstrand, strandladder, ladderwaters, watersbio, biomass, massnote, notemassprep
Application NoteImproving Recovery and Quantitation ofOligonucleotide Impurities Using ACQUITYPREMIER with MaxPeak HPS TechnologyBrooke M. Koshel, Robert E. Birdsall, Ying Qing YuWaters Corporation AbstractIon-pair reversed-phase chromatography is a commonly used technique for the separation of syntheticoligonucleotide impurities. Analysis of oligonucleotides is notoriously...
Key words
premier, premieroligonucleotide, oligonucleotideacquity, acquitymaxpeak, maxpeakhps, hpsoligonucleotides, oligonucleotidessystem, systemtechnology, technologyimpurities, impuritiespassivation, passivationconventional, conventionalsurfaces, surfacesminutes, minutesmetal, metalrecovery
 

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Application NoteImproved Oligonucleotide SPE-LC-MSAnalysis Using MaxPeak High PerformanceTechnologyKathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. RainvilleWaters Corporation AbstractThe demand for highly selective and sensitive LC-MS bioanalytical assays in support of researchand development of next-generation oligonucleotide therapies has greatly increased. The...
Key words
oligonucleotide, oligonucleotideacquity, acquityspe, spexevo, xevomaxpeak, maxpeakpremier, premieryielded, yieldeduplc, uplcbioanalytical, bioanalyticaltargetlynx, targetlynxtea, teaassays, assaysquantification, quantificationrecovery, recoverydemand
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Developing a Novel, Integrated LC-MS Workflow for High-resolutionMonitoring and Characterization of OligonucleotidesHenry Shion, Robert Birdsall, and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Optimized RP-UPLC mobile phaseMethods that enable structural characterization, molecular weightcompositions for better oligonucleotideconfirmation, and impurity analysis and...
Key words
promass, promassoligonucleotide, oligonucleotideost, ostoligonucleotides, oligonucleotidesuplc, uplcpolyt, polytacquity, acquitymasslynx, masslynxstrand, strandladder, ladderwaters, watersbio, biomass, massnote, notemassprep
Application NoteImproving Recovery and Quantitation ofOligonucleotide Impurities Using ACQUITYPREMIER with MaxPeak HPS TechnologyBrooke M. Koshel, Robert E. Birdsall, Ying Qing YuWaters Corporation AbstractIon-pair reversed-phase chromatography is a commonly used technique for the separation of syntheticoligonucleotide impurities. Analysis of oligonucleotides is notoriously...
Key words
premier, premieroligonucleotide, oligonucleotideacquity, acquitymaxpeak, maxpeakhps, hpsoligonucleotides, oligonucleotidessystem, systemtechnology, technologyimpurities, impuritiespassivation, passivationconventional, conventionalsurfaces, surfacesminutes, minutesmetal, metalrecovery
 

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