DEVELOPMENT OF A BIOANALYTICAL SPE LC-MS/MS ASSAY FOR OLIGONUCLEOTIDES
Posters | 2020 | WatersInstrumentation
Sample Preparation, Consumables, LC/MS, LC/MS/MS, LC/QQQ
IndustriesClinical Research
ManufacturerWaters
Key wordsost, massprep, area, spe, oasis, oligonucleotide, tea, wax, recovery, time, hlb, tic, oligonucleotides, bioanalytical, waters, quantification, backbones, protocol, μelution, mixed, reversed, uplc, sample, mode, hfip, elution, best, oligo, pretreated, simple, reconstitute, adsorb, ftn, development, prone, decreases, yields, endogenous, meoh, conc, cone, assays, highlights, lack, xevo, using, phase, verification, pretreatment, suitability
Similar PDF
Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology
2020|Waters|Applications
Application Note Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology Kathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. Rainville Waters Corporation Abstract The demand for highly selective and sensitive LC-MS bioanalytical assays in support of research and development…
Key words
oligonucleotide, oligonucleotidespe, speacquity, acquityxevo, xevomaxpeak, maxpeakyielded, yieldedtargetlynx, targetlynxbioanalytical, bioanalyticaluplc, uplcpremier, premiertea, teaassays, assaysquantification, quantificationdemand, demandrecovery
Improving LC-MS/MS Bioanalytical Quantitation of Oligonucleotides using New High Performance Tandem Quadrupole Mass Spectrometer Suma Veeramachineni1; Suraj Dhungana1; Ying-Qing Yu1; Mark Wrona1; 1Waters Corporation, Milford, MA Introduction Results Improving LC-MS/MS bioanalytical performance especially for ESI negative analytes has been of…
Key words
oligonucleotide, oligonucleotideaccuracy, accuracyoligonucleotides, oligonucleotidesosts, ostsperformance, performanceantisense, antisensequantitation, quantitationbioanalytical, bioanalyticallloq, lloqpolythymidine, polythymidinestd, stdpremier, premieronts, ontsconc, concwaters
Sensitive LC-MS/MS Bioanalytical Quantitation of Antisense Oligonucleotides
2022|Waters|Applications
Application Note Sensitive LC-MS/MS Bioanalytical Quantitation of Antisense Oligonucleotides Suma Veeramachineni, Mark D. Wrona Waters Corporation Abstract This application demonstrates the quantitative and qualitative capabilities of the Waters™ Xevo™ TQ Absolute Tandem Quadrupole Mass Spectrometer (MS/MS) and its suitability for…
Key words
antisense, antisenseoligonucleotides, oligonucleotidesbioanalytical, bioanalyticalsensitive, sensitivequantitation, quantitationpremier, premiertandem, tandemacquity, acquityabsolute, absolutewaters, watershps, hpsmaxpeak, maxpeakoligonucleotide, oligonucleotidehuman, humansurfaces
APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES - APPLICATION NOTEBOOK
2017|Waters|Guides
[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…
Key words
oligonucleotides, oligonucleotidesuplc, uplcoligonucleotide, oligonucleotideacquity, acquityrnai, rnaiost, ostpromass, promassduplex, duplexduplexes, duplexeswaters, waterssirna, sirnaqda, qdaanalysis, analysisrna, rnaseparation