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ASMS: Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size Exclusion Chromatography-Multi Angle Light Scattering, and Charge Detection Mass Spectrometry

Posters | 2022 | WatersInstrumentation
HPLC, LC/TOF, LC/HRMS, LC/MS, LC/MS/MS, GPC/SEC
Industries
Pharma & Biopharma
Manufacturer
Waters
Key words
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Comprehensive AAV Characterizations Using HRMS: Peptide Mapping, Capsid Proteins, Intact ssDNA, and Intact Virus T1430-09-53 Hua Yang, Ying Qing Yu, Stephan Koza, Suraj Dhungana, Ann Gray Waters Corporation, 34 Maple Street, Milford, Massachusetts, USA CONTACT INFORMATION: [email protected] PURPOSE RESULT(S) CONCLUSION(S)…
Key words
capsid, capsidaec, aecaav, aavcdms, cdmsssdna, ssdnapercent, percentfull, fullcharge, chargeintact, intactmass, massfraction, fractionempty, emptypredicted, predicteddetection, detectionprotein
Application Note Ion-Pair Reversed-Phase Liquid Chromatography Method for Analysis of mRNA Poly(A) Tail Heterogeneity Martin Gilar, Maissa M. Gaye Waters Corporation Abstract Messenger RNA (mRNA) biopolymers are new therapeutic modalities. Development of effective mRNA vaccines against SARS-CoV-2 virus accelerated the…
Key words
tail, tailmrna, mrnapoly, polyheterogeneity, heterogeneitypair, pairreversed, reversedphase, phaseliquid, liquidion, ionmethod, methodchromatography, chromatographyanalysis, analysisssystem, ssystempremier, premierdiisopropylethylamine
Application Note Size-Exclusion Chromatography Method for Poly(A) Tail Analysis of mRNA Martin Gilar Waters Corporation Abstract The first in-class therapeutic messenger RNA (mRNA) vaccines were successfully developed against SARS-CoV2 virus. Therapeutic mRNA molecules can also be used for protein replacement…
Key words
tail, tailpoly, polymrna, mrnaexclusion, exclusionsize, sizechromatography, chromatographymethod, methodanalysis, analysissec, secqsm, qsmftn, ftnpremier, premiergilar, gilaruplc, uplclength
COMPREHENSIVE AAV CHARACTERIZATIONS USING HRMS: HRMS CAPSID PROTEINS, PROTEINS, EMPTY/FULL CAPSID RATIO AND SS SS SSDNA ANALYSIS 1 Hua Yang Yang, 2Ximo Zhang, Zhang 3Benjamin Draper, Draper 4Xiaoying Jin Jin, 4Lin Liu Liu, 4Zichuan Zhang, Zhang 1Ying Qing Yu, Yu…
Key words
ssdna, ssdnacdms, cdmsdna, dnadetec, deteccapsid, capsidaec, aecaav, aavserotypes, serotypesrplc, rplcprotein, proteinpak, pakcorrela, correlaffrac, ffractheore, theorefrac
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