Mass Spectrometric Characterization of Antibody-siRNA Conjugates using the Agilent 6545XT AdvanceBio LC/Q‑TOF

Applications | 2020 | Agilent TechnologiesInstrumentation
LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
Industries
Pharma & Biopharma
Manufacturer
Agilent Technologies
Key words
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Application NotePharma & BiopharmaSensitive Native Mass Spectrometryof Macromolecules Using StandardFlow LC/MSAuthorDavid L. WongAgilent Technologies, Inc.AbstractNative mass spectrometry can be used for a variety of protein-based applications,such as protein-protein interaction, protein-ligand binding, protein complexstructures, protein folding and antibody-drug conjugates. Most of...
Key words
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Agilent-NISTmAbIntact & Subunit AnalysisUsing Reversed PhaseAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information....
Key words
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Poster ReprintASMS 2020WP 004Mass SpectrometricCharacterization of Antibody-RNAConjugates using the Agilent6545XT AdvanceBio LC/Q-TOFDavid L. WongAgilent Technologies, Inc., Santa Clara, CA,USA IntroductionExperimentalAntibody-RNA Conjugates have drug-likeThe antibody was partially reduced withproperties comparable to antibodies and allowreducing agent and was then reacted with thedelivery of...
Key words
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Application NoteBiopharma/PharmaCharacterization of Antibody‑DrugConjugates Using 2D-LC andNative MSAuthorsDavid L. Wong andSarah M. StowAgilent Technologies, Inc.AbstractAntibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb)conjugated to a small molecule drug through synthetic linkers, have been afast-growing class of effective biotherapeutics in...
Key words
dimension, dimensiondar, daradcs, adcsadc, adcsecond, secondnative, nativehic, hicadvancebio, advancebiocounts, countsmab, mabdeconvoluted, deconvoluteddrug, drugfirst, firstconjugated, conjugatedintact