Mass Spectrometric Characterization of Antibody-siRNA Conjugates using the Agilent 6545XT AdvanceBio LC/Q‑TOF | LabRulez LCMS
 

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Application NotePharma & BiopharmaSensitive Native Mass Spectrometryof Macromolecules Using StandardFlow LC/MSAuthorDavid L. WongAgilent Technologies, Inc.AbstractNative mass spectrometry can be used for a variety of protein-based applications,such as protein-protein interaction, protein-ligand binding, protein complexstructures, protein folding and antibody-drug conjugates. Most of...
Key words
native, nativeprotein, proteincounts, countsintact, intactdeconvoluted, deconvolutedmyoglobin, myoglobinamu, amumass, masscharge, chargeapomyoglobin, apomyoglobincomplexes, complexesheme, hemeholomyoglobin, holomyoglobindenatured, denaturedtetramer
Agilent-NISTmAbIntact & Subunit AnalysisUsing Reversed PhaseAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information....
Key words
counts, countsmab, mabherceptin, herceptinintact, intactpage, pagecontents, contentsbonds, bondsback, backdeglycosylated, deglycosylatedmass, massdeconvoluted, deconvolutedides, idesamu, amubravo, bravoassaymap
Poster ReprintASMS 2020WP 004Mass SpectrometricCharacterization of Antibody-RNAConjugates using the Agilent6545XT AdvanceBio LC/Q-TOFDavid L. WongAgilent Technologies, Inc., Santa Clara, CA,USA IntroductionExperimentalAntibody-RNA Conjugates have drug-likeThe antibody was partially reduced withproperties comparable to antibodies and allowreducing agent and was then reacted with thedelivery of...
Key words
conjugates, conjugatesrna, rnamab, mabantibody, antibodycondition, conditionadvancebio, advancebiosirna, sirnaunder, undernative, nativedenaturing, denaturingthiomab, thiomabdelivery, deliveryintact, intactspanned, spannedpayloads
Application NoteBiopharma/PharmaCharacterization of Antibody‑DrugConjugates Using 2D-LC andNative MSAuthorsDavid L. Wong andSarah M. StowAgilent Technologies, Inc.AbstractAntibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb)conjugated to a small molecule drug through synthetic linkers, have been afast-growing class of effective biotherapeutics in...
Key words
dimension, dimensiondar, daradc, adcadcs, adcssecond, secondnative, nativehic, hicadvancebio, advancebiocounts, countsfirst, firstmab, mabdeconvoluted, deconvoluteddrug, drugconjugated, conjugatedintact
 

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Application NotePharma & BiopharmaSensitive Native Mass Spectrometryof Macromolecules Using StandardFlow LC/MSAuthorDavid L. WongAgilent Technologies, Inc.AbstractNative mass spectrometry can be used for a variety of protein-based applications,such as protein-protein interaction, protein-ligand binding, protein complexstructures, protein folding and antibody-drug conjugates. Most of...
Key words
native, nativeprotein, proteincounts, countsintact, intactdeconvoluted, deconvolutedmyoglobin, myoglobinamu, amumass, masscharge, chargeapomyoglobin, apomyoglobincomplexes, complexesheme, hemeholomyoglobin, holomyoglobindenatured, denaturedtetramer
Agilent-NISTmAbIntact & Subunit AnalysisUsing Reversed PhaseAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information....
Key words
counts, countsmab, mabherceptin, herceptinintact, intactpage, pagecontents, contentsbonds, bondsback, backdeglycosylated, deglycosylatedmass, massdeconvoluted, deconvolutedides, idesamu, amubravo, bravoassaymap
Poster ReprintASMS 2020WP 004Mass SpectrometricCharacterization of Antibody-RNAConjugates using the Agilent6545XT AdvanceBio LC/Q-TOFDavid L. WongAgilent Technologies, Inc., Santa Clara, CA,USA IntroductionExperimentalAntibody-RNA Conjugates have drug-likeThe antibody was partially reduced withproperties comparable to antibodies and allowreducing agent and was then reacted with thedelivery of...
Key words
conjugates, conjugatesrna, rnamab, mabantibody, antibodycondition, conditionadvancebio, advancebiosirna, sirnaunder, undernative, nativedenaturing, denaturingthiomab, thiomabdelivery, deliveryintact, intactspanned, spannedpayloads
Application NoteBiopharma/PharmaCharacterization of Antibody‑DrugConjugates Using 2D-LC andNative MSAuthorsDavid L. Wong andSarah M. StowAgilent Technologies, Inc.AbstractAntibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb)conjugated to a small molecule drug through synthetic linkers, have been afast-growing class of effective biotherapeutics in...
Key words
dimension, dimensiondar, daradc, adcadcs, adcssecond, secondnative, nativehic, hicadvancebio, advancebiocounts, countsfirst, firstmab, mabdeconvoluted, deconvoluteddrug, drugconjugated, conjugatedintact
 

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