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Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
Key words
dispersion, dispersionfeatherstone, featherstonecourt, courtmill, millroad, road𝑡𝑅, 𝑡𝑅zdv, zdvmeasured, measured𝑆𝑦𝑠𝑡𝑒𝑚, 𝑆𝑦𝑠𝑡𝑒𝑚𝑉𝑜𝑙𝑢𝑚𝑒, 𝑉𝑜𝑙𝑢𝑚𝑒yield, yieldvolume, volumezero, zerounion, uniondwell
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadionisable, ionisable𝑅𝑠, 𝑅𝑠alter, altermodifier, modifierorganic, organicresolution, resolutioncan, canequation, equationdifferent, different𝑑𝑝, 𝑑𝑝resolved
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadgradient, gradient𝑡𝐺, 𝑡𝐺ɸ𝑉𝑚𝑆, ɸ𝑉𝑚𝑆𝑃𝐶, 𝑃𝐶anatomy, anatomyalters, altersretentivity, retentivitypitfalls, pitfallsbasics, basicschromatography, chromatographyexperimentally
Back to BasicsPump Linearity and DwellVolume MeasurementsProtocols for LC Instruments2020How is the Linearity of the Pump Measured?The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. Thisis in order to ascertain the ability...
Key words
featherstone, featherstonecourt, courtdwell, dwellmill, millroad, roadmau, maucorr, corrwater, waterpump, pumpzdv, zdvvolume, volumeabs, absmeasured, measuredisocratic, isocraticgradient
 

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Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
Key words
dispersion, dispersionfeatherstone, featherstonecourt, courtmill, millroad, road𝑡𝑅, 𝑡𝑅zdv, zdvmeasured, measured𝑆𝑦𝑠𝑡𝑒𝑚, 𝑆𝑦𝑠𝑡𝑒𝑚𝑉𝑜𝑙𝑢𝑚𝑒, 𝑉𝑜𝑙𝑢𝑚𝑒yield, yieldvolume, volumezero, zerounion, uniondwell
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadionisable, ionisable𝑅𝑠, 𝑅𝑠alter, altermodifier, modifierorganic, organicresolution, resolutioncan, canequation, equationdifferent, different𝑑𝑝, 𝑑𝑝resolved
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadgradient, gradient𝑡𝐺, 𝑡𝐺ɸ𝑉𝑚𝑆, ɸ𝑉𝑚𝑆𝑃𝐶, 𝑃𝐶anatomy, anatomyalters, altersretentivity, retentivitypitfalls, pitfallsbasics, basicschromatography, chromatographyexperimentally
Back to BasicsPump Linearity and DwellVolume MeasurementsProtocols for LC Instruments2020How is the Linearity of the Pump Measured?The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. Thisis in order to ascertain the ability...
Key words
featherstone, featherstonecourt, courtdwell, dwellmill, millroad, roadmau, maucorr, corrwater, waterpump, pumpzdv, zdvvolume, volumeabs, absmeasured, measuredisocratic, isocraticgradient
 

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