Back to Basics - Explaining ResolutionTechnické články | 2020 | ShimadzuInstrumentationHPLCKey wordsfeatherstone, court, mill, road, ionisable, 𝑅𝑠, alter, modifier, organic, resolution, can, equation, different, 𝑑𝑝, resolved, buffer, normalised, explaining, minimising, ionised, offer, baseline, stationary, phases, composition, utilising, retentivity, what, which, effective, basics, altering, remember, tpp, column, elucidate, dramatic, formats, type, fundamental, broadening, mechanisms, plotted, separately, dispersion, assist, most, sizes, retention, one
Back to Basics - Gradient Retention Factor, K*
Back to BasicsGradient Retention Factor, K*2020What is Gradient Retention Factor, k*?In isocratic separations, analyte retention time can be normalised to retention factor, k, whichallows a direct comparison between columns with different dimensions. This is possible due to theconstant mobile phase...
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Back to Basics - Dispersion - Protocols for LC Instruments
Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
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Back to Basics - Gradient Anatomy - Protocols for LC Instruments
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
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Back to Basics - Pump Linearity and Dwell Volume Measurements - Protocols for LC Instruments
Back to BasicsPump Linearity and DwellVolume MeasurementsProtocols for LC Instruments2020How is the Linearity of the Pump Measured?The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. Thisis in order to ascertain the ability...
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