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Difluoroacetic Acid as a Mobile Phase Modifier for LC-MS Analysis of Small Molecules

 

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[ TECHNOLOGY BRIEF ] Application of Difluoroacetic Acid to Improve Optical and MS Performance in Peptide LC-UV/MS Jacob Kellett, Robert Birdsall, and Ying Qing Yu Waters Corporation, Milford, MA, USA Use of difluoroacetic acid as an ion-pairing agent improves optical…
Key words
pairing, pairingdifluoroacetic, difluoroaceticoptical, opticalpeptide, peptideagent, agentacid, acidtrifluoroacetic, trifluoroaceticrplc, rplctfa, tfadfa, dfaion, ionionhance, ionhanceformic, formicresponse, responsebrief
[ APPLICATION NOTE ] Comparing Mobile Phase Additives for the Separation of mAb Tryptic Peptides: A Case Study on Formic, Difluoroacetic, and Trifluoroacetic Acid Jennifer M. Nguyen, Xiaoxiao Liu, and Matthew A. Lauber Waters Corporation, Milford, MA, USA APPLICATION BENEFITS…
Key words
dfa, dfaseparations, separationstfa, tfatryptic, trypticmab, mabcsh, cshpeptide, peptidepeptides, peptidesbeh, behadditives, additivescomparing, comparingmobile, mobiledeamidated, deamidatedphase, phasedifluoroacetic
UNIVERSITY OF COPENHAGEN FA C U LT Y O F S C I E N C E Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing Jennifer M. Nguyen1,2, Xiaoxiao Liu2, and Matthew A. Lauber2 1University of Copenhagen, Frederiksberg,…
Key words
dfa, dfatfa, tfapeptide, peptidecarbamidomethyl, carbamidomethylstate, statetryptic, trypticbeh, behsodium, sodiumcharge, chargeminimizing, minimizingbpi, bpimapping, mappingmab, mabmobile, mobilepotassium
Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing Jennifer Nguyen, Xiaoxiao Liu, and Matthew A. Lauber Waters Corporation, Milford, MA, USA INTRODUCTION Peptide mapping is an important and critical technique in the characterization of biopharmaceuticals. These separations are…
Key words
dfa, dfapeptide, peptidecarbamidomethyl, carbamidomethylseparations, separationstfa, tfaabundances, abundancesdifluoroacetic, difluoroacetictryptic, trypticcharge, chargeionhance, ionhancepurified, purifiedminimizing, minimizingmab, mabalkylated, alkylatedform
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