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Surviving Chromatography - Part 1: Preventive Action
2019|Agilent Technologies|Prezentace
Surviving ChromatographyPart 1: Preventive ActionMark PowellAlexander Ucci1September 10, 2018Agilent restricted Planning Ahead• Planning and good habits can help preventproblems that can take you down the wrongpath and cause you to lose yourchromatography• Care of your instrument:• supplies• sample prep• instrument configuration•...
Key words
restricted, restrictedagilent, agilentporoshell, poroshellinfinitylab, infinitylabvendor, vendorrotor, rotorseal, seallamps, lampsother, otherlipid, lipidfilters, filtersemr, emrcolumn, columnseals, sealsless
Thinking Outside the C18 Box
2018|Agilent Technologies|Prezentace
Thinking Outside the C18 Box1 Modes of HPLC• Reversed-phase• C18, C8, other non-polar phases• Phenyl, PFP, other more polar phases• Ion Pairing• HILIC• Silica• Amide• Zwitterionic• Hydroxyl-based• Chiral• Normal phase(Rx-SIL)• Silica• Aminopropyl(Hi-Plex)• Ion Exclusion• Sulfonated polystyrene/divinylbenzene• Ion Exchange (Bio IEX)• Anion...
Key words
hilic, hilicporoshell, poroshellpolar, polarvitamins, vitaminsphase, phasephases, phasesbest, bestmobile, mobilesoluble, solublechiral, chiralconfidentiality, confidentialitypair, pairstatement, statementwater, waterion
The LC Column Playbook
2017|Agilent Technologies|Prezentace
The LC ColumnPlaybookMark PowellApplications EngineerTechnical SupportOctober 10, 20171October 10, 2017 OverviewHow do we achieve our goal:Resolution?• Efficiency• Particle size• Column length• Selectivity• Bonded phase• Mobile phase• Retention• Polar compounds• Polar bonded phase• HILIC2October 10, 2017For Research Use Only. Not for use...
Key words
hilic, hilicporoshell, poroshellmau, maupolar, polarselectivity, selectivitybest, bestphases, phasesresolution, resolutionphase, phaseparticle, particlemin, minmobile, mobileⅆ𝑝, ⅆ𝑝bonded, bondedconfidentiality
What Do I Need for Successful Method Development?
2020|Agilent Technologies|Prezentace
What Do I Need forSuccessful MethodDevelopment?Mark PowellColumns and Supplies Technical SupportDecember 10, 2020 What is My Method Development Plan?1.Smaller particles and superficially porous particles offer fast, efficient analysis2.C18 column – general-purpose column choice3.Simple mobile phasea) Formic acid or other additive in...
Key words
what, whatsuccessful, successfuldevelopment, developmentneed, needmethod, methodyes, yesmau, mauabsorbance, absorbanceinfinitylab, infinitylabmobile, mobilechoose, choosephase, phasebonded, bondedvolume, volumeparticle
 

Similar PDF

Surviving Chromatography - Part 1: Preventive Action
2019|Agilent Technologies|Prezentace
Surviving ChromatographyPart 1: Preventive ActionMark PowellAlexander Ucci1September 10, 2018Agilent restricted Planning Ahead• Planning and good habits can help preventproblems that can take you down the wrongpath and cause you to lose yourchromatography• Care of your instrument:• supplies• sample prep• instrument configuration•...
Key words
restricted, restrictedagilent, agilentporoshell, poroshellinfinitylab, infinitylabvendor, vendorrotor, rotorseal, seallamps, lampsother, otherlipid, lipidfilters, filtersemr, emrcolumn, columnseals, sealsless
Thinking Outside the C18 Box
2018|Agilent Technologies|Prezentace
Thinking Outside the C18 Box1 Modes of HPLC• Reversed-phase• C18, C8, other non-polar phases• Phenyl, PFP, other more polar phases• Ion Pairing• HILIC• Silica• Amide• Zwitterionic• Hydroxyl-based• Chiral• Normal phase(Rx-SIL)• Silica• Aminopropyl(Hi-Plex)• Ion Exclusion• Sulfonated polystyrene/divinylbenzene• Ion Exchange (Bio IEX)• Anion...
Key words
hilic, hilicporoshell, poroshellpolar, polarvitamins, vitaminsphase, phasephases, phasesbest, bestmobile, mobilesoluble, solublechiral, chiralconfidentiality, confidentialitypair, pairstatement, statementwater, waterion
The LC Column Playbook
2017|Agilent Technologies|Prezentace
The LC ColumnPlaybookMark PowellApplications EngineerTechnical SupportOctober 10, 20171October 10, 2017 OverviewHow do we achieve our goal:Resolution?• Efficiency• Particle size• Column length• Selectivity• Bonded phase• Mobile phase• Retention• Polar compounds• Polar bonded phase• HILIC2October 10, 2017For Research Use Only. Not for use...
Key words
hilic, hilicporoshell, poroshellmau, maupolar, polarselectivity, selectivitybest, bestphases, phasesresolution, resolutionphase, phaseparticle, particlemin, minmobile, mobileⅆ𝑝, ⅆ𝑝bonded, bondedconfidentiality
What Do I Need for Successful Method Development?
2020|Agilent Technologies|Prezentace
What Do I Need forSuccessful MethodDevelopment?Mark PowellColumns and Supplies Technical SupportDecember 10, 2020 What is My Method Development Plan?1.Smaller particles and superficially porous particles offer fast, efficient analysis2.C18 column – general-purpose column choice3.Simple mobile phasea) Formic acid or other additive in...
Key words
what, whatsuccessful, successfuldevelopment, developmentneed, needmethod, methodyes, yesmau, mauabsorbance, absorbanceinfinitylab, infinitylabmobile, mobilechoose, choosephase, phasebonded, bondedvolume, volumeparticle
 

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