KNAUER Eurospher II 100 HILIC (U)HPLC Columns

Zwitterionic and polar columns for HILIC applications

Based on ultra-pure Silica gel, zwitterionic HILIC phases are ideal for the analysis of polar and ionizable compounds. Stronger interaction of more polar compounds with stationary aqueous layer.

Faster elution of nonpolar compounds is possible due to minor hydrophobic interactions. Retention increases with the charge of the analyte.

Recommended for the analysis of hydrophilic compounds like:

• Polar organic acids and bases
• Natural polar compounds
• Nucleosides
• Oligonucleotides
• Amino acids
• Peptides
• Water soluble vitamins

Properties

• Separation mechanism: Hydrophilic and weak electrostatic interactions
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Zwitterionic modification: Ammonium – sulfonic acid
• 7 % carbon content

Key features

• Modern HILIC phase with zwitterionic modification on the basis of ammonium- sulfonic acid. Outstanding mechanical and chemical stability.

Recommended application areas

• Especially suited for the separation of hydrophilic, polar and ionic analytes which are poorly retained on reversed phase columns; behavior is the other way round on Eurospher II HILIC compared to RP which makes it an ideal tool to enhance chromatographic separations for these molecules.

KNAUER: HILIC

Tip: HILIC applications are well-suited for coupling with MS detection. The high organic content of the applied mobile phases are qualified well for evaporation in the MS´s ionization source.

Stationary phase Eurospher II 100 HILIC is provided in these column types: analytical

Eurospher II 100 HILIC Analytical Columns are available with these parameters:

• Column length (mm): 50, 100, 150, 250
• Column ID (mm): 3, 4, 4.6
• Particle size (µm): 3, 5

KNAUER (U)HPLC Columns

Widest range of HPLC and UHPLC columns for all separation modes and applications.

• Safe Investment: Robust and reliable columns for attractive price 
• Separation Mode: Reversed Phase, HILIC, Normale
• Simplicity: Easy purchase via our online store with fast delivery
• Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
• Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar

KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.

KNAUER: KNAUER (U)HPLC Columns