KNAUER Eurospher II 100 C18A Prep HPLC Columns (USP L1)

C18A columns for polar reversed phase applications with 100 % aqueous mobile phase (USP L1)

C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.

Properties

• Separation mechanism: Hydrophobic and polar interaction
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Polar, monomeric C18 (Octadecyl) modification, polar endcapping, with 10 % carbon content (~ 50 % endcapping).

Key features

• C18 A phase with alternative, more polar selectivity, suited for the use with pure aqueous mobile phases, 50 % endcapping and resulting 10 % carbon content, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications

Recommended application areas

• Polar endcapped C18 phase for water soluble and polar analytes, 100 % aqueous eluents for analysis of very polar compounds:
  • basic pharmaceutical ingredients
  • water soluble vitamins
  • catecholamines
  • as well as organic acids.

KNAUER: C18A

Tip: With an endcapping the influence of nonderivatized silanol groups can be minimized. The endcapping reagent is generally a smaller silane than used for derivatisation. This treatment reduces the unwanted interaction of polar or charged analytes (acids, bases ect.) because the amount of available silanol groups is reduced.

Stationary phase Eurospher II 100 C18A is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 C18A Preparative Standard Columns are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 20, 30, 50
• Particle size (µm): 5, 10, 15

Eurospher II 100 C18A Preparative dAX Columns with dynamic axial compression are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 25, 30, 50
• Particle size (µm): 5, 10, 15

KNAUER Preparative HPLC Columns

The right choice for all dimensions from semi-prep to batch chromatography to SMB.

• Modifications: Reversed Phase, HILIC, Normale Phase
• Max. Pressure: 400–600 bar
• Column Inner Diameter (ID): 16–50 mm in our shop, more on request
• Column Brands: Eurospher, Eurospher II
• Safe Investment: Robustness and durability based on superior technology
• Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
• Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available

KNAUER: KNAUER Preparative HPLC Columns

Preparative HPLC columns can be offered with two different hardware types:

• Versatile standard flanged hardware for nearly any application task in batch chromatography
• dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes