EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety

<div><div>Researchers led by <a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en">Eva Kudová</a> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant Staphylococcus aureus.</div></div><div>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub">Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</a></h4>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;Eur. J. Med. Chem., 2026, 308, 118716<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant Staphylococcus aureus. Structure-activity relationship analysis identified compounds 13 and 16 as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound 13 significantly downregulated S. aureus virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.<h4>Instrumentation</h4><h5>Structural characterization</h5><ul><li>AVANCE III 400 MHz NMR spectrometer (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL">LTQ Orbitrap XL HRMS</a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

Metformin is currently the first-line oral drug of choice for the treatment of type 2 diabetes. It reduces glucose production in the liver, improves tissue sensitivity to insulin, and does not cause weight gain or hypoglycemia, which sets it apart from some other antidiabetic drugs. Moreover, its long-term beneficial effects on the risk of cardiovascular complications and mortality in people with type 2 diabetes have been well established. Researchers from the Institute of Physiology CAS (IPHYS) have identified a new group of blood metabolites that could help physicians objectively monitor whether patients with type 2 diabetes are taking their metformin as prescribed.<h4>New biomarkers for metformin use</h4>A team led by Tomáš Čajka from the Laboratory of Translational Metabolism at IPHYS analyzed plasma samples from 637 patients with type 2 diabetes treated with metformin and 143 non-diabetic controls using advanced metabolomics and lipidomics. Among 614 detected metabolites, they identified five from the group of N-lactoyl-amino acids (e.g. N‑lactoyl-phenylalanine), which had previously been seen as unknown signals due to missing reference spectra in databases. The levels of these metabolites in the blood closely reflected the plasma concentrations of metformin. In patients with the highest metformin exposure, the concentrations of these metabolites were up to 7.2 times higher than in controls.<h4>A sensitive “fingerprint” of the drug’s effect</h4>N-Lactoyl-amino acids are formed in cells by combining lactate and amino acids, thus reflecting the metformin-induced changes in cellular energy metabolism. “Importantly, even though they occur in plasma only at nanomolar concentrations, they reliably distinguish patients who take metformin regularly from those who take it irregularly or not at all,” emphasizes T. Čajka.<h4>Metabolomics in practice and international collaboration</h4>The study also provides a detailed description of broader metabolic changes in type 2 diabetes, including increases in lactate, organic acids, and branched-chain amino acids, as well as extensive remodelling of the lipid profile. In addition to T. Čajka’s team, collaborators from the Institute for Clinical and Experimental Medicine in Prague and the West Coast Metabolomics Center (University of California, Davis) also participated in the study.The results demonstrate that advanced metabolomics approaches developed at IPHYS within the CarDia project can find highly practical applications both in clinical studies and in personalized monitoring of treatment in patients with diabetes.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://ascpt.onlinelibrary.wiley.com/doi/10.1002/cpt.70205">Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes</a></h4>Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Veronika Hola, Stanislava Rakusanova, Oliver Fiehn, Vojtech Skop, Ivana Lankova, Iva Miskova, Terezie Pelikanova, Martin HaluzikClin Pharmacol Ther, 119: 1057-1069<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/cpt.70205">https://doi.org/10.1002/cpt.70205</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Metformin is the most widely prescribed antidiabetic drug, yet adherence remains difficult to objectively assess. Using untargeted metabolomics and lipidomics, we analyzed plasma from 637 patients with type 2 diabetes (T2D) with confirmed metformin use and 143 nondiabetic controls, annotating 614 metabolites. Patients were stratified by plasma metformin into sub-therapeutic, therapeutic, and supra-therapeutic groups, and associations were evaluated by multiple linear regression and composite metabolite ranking. Five previously unannotated features were structurally identified as N-lactoyl-amino acids, whose levels correlated strongly with plasma metformin (ρ = 0.42–0.55, P &lt; 0.0001) and increased up to 7.2-fold in the supra-therapeutic group (&gt; 2000 ng/mL). While N-lactoyl-amino acids were consistently detected in the nanomolar range, they still displayed robust and dose-dependent associations with metformin. Broader metabolic changes in T2D included elevated lactate, organic acids, and branched-chain amino acids, together with reduced urea cycle metabolites. Lipidomics showed increases in saturated triacylglycerols and diacylglycerols and decreases in cholesteryl esters, sphingomyelins, and phospholipids. These findings establish N-lactoyl-amino acids as robust, dose-responsive plasma biomarkers of metformin exposure. Despite being up to four orders of magnitude less abundant than their amino acid precursors, they sensitively reflect mitochondrial lactate overflow and pharmacodynamic adaptation, offering objective assessment of adherence.

New blood biomarkers reveal how faithfully diabetes patients take metformin

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The Czech Academy of Sciences (CAS) was established by Act No. 283/1992 Coll. as the Czech successor of the former Czechoslovak Academy of Sciences. It is set up as a complex of 54 public research institutions.

Czech Academy of Sciences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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KNAUER Eurospher II 100 C18 Prep HPLC Columns (USP L1)

Stationary phase Eurospher II 100 C18 Prep HPLC is the most often used stationary phase in reversed phase mode

Classical Reversed Phase columns for HPLC and UHPLC (USP L1)Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.<h5>Properties</h5><ul><li>Separation mechanism: Hydrophobic interaction</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Unpolar, monomeric C18 (Octadecyl) modification, endcapped, with 16 % carbon content (~ 50 % endcapping).</li></ul><h5>Key features</h5><ul><li>High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical C18 phase with ca. 50 % endcapping and resulting 16 % carbon content, outstanding mechanical and chemical stability.</li></ul><h5>Recommended application areas</h5><ul><li>acidic, basic and neutral analytes in reversed phase mode, for example sulphonamides</li><li>anabolic steroids</li><li>anti-psychotics</li><li>beta blocker</li><li>sudan dyes</li><li>phenols and preservatives</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C18_non_endcapped_6e4fce9989.webp" alt="KNAUER: C18 Non-endcapped" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C18_non_endcapped_6e4fce9989.webp 245w," sizes="100vw" width="682" height="420"><blockquote>Tip: Caution! Never use classical C18 phases with 100 % aqueous mobile phase. The hydrophobic C18 chains will collapse</blockquote>Stationary phase Eurospher II 100 C18 is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 C18 Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 C18 Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC columns can be offered with two different hardware types:</h5><ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

KNAUER Eurospher II 100 C18A Prep HPLC Columns (USP L1)

C18A phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases

C18A columns for polar reversed phase applications with 100 % aqueous mobile phase (USP L1)C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.<h5>Properties</h5><ul><li>Separation mechanism: Hydrophobic and polar interaction</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Polar, monomeric C18 (Octadecyl) modification, polar endcapping, with 10 % carbon content (~ 50 % endcapping).</li></ul><h5>Key features</h5><ul><li>C18 A phase with alternative, more polar selectivity, suited for the use with pure aqueous mobile phases, 50 % endcapping and resulting 10 % carbon content, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications</li></ul><h5>Recommended application areas</h5><ul><li>Polar endcapped C18 phase for water soluble and polar analytes, 100 % aqueous eluents for analysis of very polar compounds:<ul><li>basic pharmaceutical ingredients</li><li>water soluble vitamins</li><li>catecholamines</li><li>as well as organic acids.</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C18_A_6f4b0c3063.webp" alt="KNAUER: C18A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C18_A_6f4b0c3063.webp 245w," sizes="100vw" width="690" height="428"><blockquote>Tip: With an endcapping the influence of nonderivatized silanol groups can be minimized. The endcapping reagent is generally a smaller silane than used for derivatisation. This treatment reduces the unwanted interaction of polar or charged analytes (acids, bases ect.) because the amount of available silanol groups is reduced.</blockquote>Stationary phase Eurospher II 100 C18A is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 C18A Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 C18A Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC columns can be offered with two different hardware types:</h5><ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

KNAUER Eurospher II 100 C18H Prep HPLC Columns (USP L1)

C18H stationary phase with a special, high efficiency or polymeric unpolar endcapping style. Suited for the use of mobile phase in an extended pH range from typically pH 1–12. 

Hydrophobic / pH stable C18H Columns for more unpolar analytes in Reversed Phase (USP L1)C18H stationary phase with a special, high efficiency or polymeric unpolar endcapping style. Because of the high carbon content and the protection of the Silica gel these materials are also suited for the use of mobile phase in an extended pH range from typically pH 1–12.While a high endcapped C18H phase is characterized mostly by the high carbon content. A polymeric endcapped phase also provides an increased steric selectivity.<h5>Properties</h5><ul><li>Separation mechanism: Hydrophobic interaction</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Nonpolar, monomeric C18 (Octadecyl) modification, high efficiency endcapping with 17 % carbon content (~ 99 % endcapping)</li></ul><h5>Key features</h5><ul><li>Nonpolar C18 phase with high efficiency endcapping and 17 % carbon content, resulting in higher pH stability in the range of 1–12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.</li></ul><h5>Recommended application areas</h5><ul><li>Recommended alternative to Kromasil 100 C18, for acidic, basic and neutral analytes in reversed phase mode with extended pH range.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C18_H_c5531f7d87.webp" alt="KNAUER: C18H" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C18_H_c5531f7d87.webp 226w," sizes="100vw" width="687" height="475"><blockquote>Tip: All analytical KNAUER columns can be used in reversed flow direction for example for cleaning. Please refer to our Column care and use documents on the website for further information about storage conditions and cleaning procedures.</blockquote>Stationary phase Eurospher II 100 C18H is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 C18H Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 C18H Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC columns can be offered with two different hardware types:</h5><ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

KNAUER Eurospher II 100 C18P Prep HPLC Columns (USP L1)

KNAUER´s most unpolar C18P phase with excellent shape selectivity. It features polymeric, high efficiency endcapping of 99 % and 20 % carbon content, exceptional pH stability in the range of 1–12.

C18P columns with trifunctional modifications (USP L1): Hydrophobic and stearic interactionsKNAUER´s most unpolar C18P phase with excellent shape selectivity. It features polymeric, high efficiency endcapping of 99 % and 20 % carbon content, exceptional pH stability in the range of 1–12, and outstanding mechanical and chemical stability.<h5>Properties</h5><ul><li>Separation mechanism: Hydrophobic and stearic interaction</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Unpolar, trifunctional C18 (Octadecyl) modification, high efficiency endcapping, with 20 % carbon content (~ 99 % endcapping)&nbsp;</li></ul><h5>Key features</h5><ul><li>KNAUER´s most unpolar C18 phase, polymeric, high efficiency endcapping of 99 % and 20 % carbon content, higher pH stability in the range of 1-12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.</li></ul><h5>Recommended application areas</h5><ul><li>Alternative selectivity to standard C18; stationary phase in Eurospher II C18 family with the highest carbon load; fully endcapped; excellent shape selectivity and pH stability.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C18_P_f093126fa9.webp" alt="KNAUER: C18P" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C18_P_f093126fa9.webp 245w," sizes="100vw" width="278" height="174"><blockquote>Tip: Not all C18 phases are comparable! When replacing an existing column, always have a close look at the characteristics like carbon load, pore size and specific surface area.</blockquote>Stationary phase Eurospher II 100 C18P is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 C18P Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 C18P Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC columns can be offered with two different hardware types:</h5><ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

Thermo Scientific Syncronis C18 HPLC Columns

Attain repeatable and consistent LC separations for your scale up purification needs with Thermo Scientific™ Syncronis™ C18 reversed phase preparative HPLC columns. 

Attain repeatable and consistent LC separations for your scale up purification needs with Thermo Scientific™ Syncronis™ C18 reversed phase preparative HPLC columns. These preparative HPLC columns have extremely pure, high surface area silica with dense stationary phase coverage to minimize secondary interactions and ensure robust, high-resolution separations. Double endcapping provides additional surface coverage and inertness toward basic compounds. Columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.<h5>Highly Reproducible Separations</h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5>Enhanced Resolution and Sensitivity</h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing.</li><li>Double endcapped for extra surface coverage. C18 (USP L1) selectivity.</li></ul>Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.<h5>Standard Threaded and Standard Flange Hardware</h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5>XtendedLife Hardware</h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.<h5>Customer Specific Orders</h5>If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements. Please contact your local sales representative for Customer Specific Orders. Custom built columns are not eligible for returns.<h4>Specifications</h4><ul><li>Column type: Reversed Phase</li><li>Endcapped: yes</li><li>Max. Pressure: 4300 psi (295 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: C18</li><li>USP Type: L1</li><li>Surface Area: 320 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 16%</li><li>Phase: Reversed Phase</li><li>Pore Size: 100 Å</li><li>pH: 2 to 9</li></ul><h4>Syncronis HPLC and UHPLC Columns</h4><h5>Enter a new world of chromatography</h5>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.<h5>Features of Syncronis columns</h5>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:<ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Syncronis PREP HILIC HPLC Columns

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes.

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations.&nbsp;The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. Our columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.<h5>Alternative Selectivity</h5><ul><li>Zwitterionic bonded phase enhances retention of polar and hydrophilic analytes.</li><li>No need for ion-pair or derivatization.</li><li>Enhanced sensitivity with MS detection.</li><li>Highly organic mobile phase facilitates desolvation in ESI-MS</li></ul>Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.<h5>Standard Threaded and Standard Flange Hardware</h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5>XtendedLife Hardware</h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.<h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Endcapped: No</li><li>Max. Pressure: 2000 psi (140 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Zwitterionic</li><li>Surface Area: 320 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 5%</li><li>Phase: HILIC</li><li>Pore Size: 100 Å</li><li>pH: 2 to 8</li></ul><h4>Syncronis HPLC and UHPLC Columns</h4><h5>Enter a new world of chromatography</h5>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.<h5>Features of Syncronis columns</h5>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:<ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Phenomenex Gemini C18 HPLC Columns

Gemini C18 is a robust reversed-phase HPLC stationary phase offering unique selectivity, high structural integrity, and increased loadability, making it ideal for preparative and purification applications.

Gemini C18 HPLC phase offers unique reversed phase selectivity, high structural integrity, and increased loadability which make it ideal for preparative and purification applications in pre-packed columns and bulk media.<ul><li>Carbon Load: 14, 14 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: Separation of basic compounds under high pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini NX-C18 HPLC Columns

Gemini NX-C18 is a rugged, high-efficiency reversed-phase HPLC column delivering controlled selectivity for ionizable compounds and reliable performance across high and low pH LC/MS workflows.

The most rugged and highly efficient Gemini C18 HPLC column phase for reversed phase. Gemini NX-C18 takes LC/MS separations to the next level and offers:<ul><li>Control selectivity of ionizable compounds for optimized methods</li><li>Consistent performance in both volatile and non-volatile buffers</li><li>High and low pH methods</li></ul><h5>Key features:</h5><ul><li>Carbon Load:14 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: High efficiency analytical or preparative separations under extreme pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4>Optimized Purification Media for Human Insulin and It's Analogues</h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.<h4>QC Tested to ensure consistent selectivity for Insulin and Impurity</h4>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.<h5>Key features:</h5><ul><li>Carbon Load: -</li><li>Surface Area (m²/g): -</li><li>Particle Size: 10</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini C6-Phenyl HPLC Columns

Gemini C6-Phenyl HPLC phase provides aromatic selectivity with low bleed performance, making it ideal for LC/MS analysis of aromatic, polar, and basic compounds under high pH conditions.

Gemini C6-Phenyl HPLC phase has a phenyl group bound to the organo-silica surface using a 6-carbon chain for a low bleed aromatic selective phase. Gemini C6-Phenyl is well suited for LC/MS applications.<h5>Key features:</h5><ul><li>Carbon Load: 12 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5</li><li>Recommended Use: Separation of aromatic, polar, or basic compounds under high pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Products from category Preparative HPLC columns - page 1

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