KNAUER Eurospher II 100 C8 & C8A Prep HPLC Columns (USP L7)

Classical and hydrophilic/aqueous C8 columns for HPLC and UHPLC (USP L7)

C8 is a silica gel based stationary phase in reversed phase mode with alternative selectivity compared to C18 phases. Compared to C18 phases, these columns show less retention due to the lower hydrophobicity.

• Ultra pure, spherical high performance HPLC phase based on silica gel
• Monomeric C8 (Octyl) modification, standard endcapping, with 10 % carbon content (~ 50 % endcapping)

Properties

• Separation mechanism: Hydrophobic interaction(lower compared to C18 phases)

Key features

• Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.

Recommended application areas

• Similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of:
  • Water soluble vitamins
  • Steroids
  • Catecholamines
  • Sedatives

KNAUER: C8 RP Non-endcapped

Hydrophilic C8A phase offers an alternative, more polar selectivity. It is suited for highly polar reversed phase applications with up to 100 % aqueous mobile phases. 

KNAUER: C8A

Tip: Caution! Even for short term storage all buffer solutions have to be rinsed from the column to prevent crystallization effects.

Stationary phases Eurospher II 100 C8 and C8A are provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 C8 and C8A Preparative Standard Columns are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 20, 30, 50
• Particle size (µm): 5, 10, 15

Eurospher II 100 C8 and C8A Preparative dAX Columns with dynamic axial compression are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 25, 30, 50
• Particle size (µm): 5, 10, 15

KNAUER Preparative HPLC Columns

The right choice for all dimensions from semi-prep to batch chromatography to SMB.

• Modifications: Reversed Phase, HILIC, Normale Phase
• Max. Pressure: 400–600 bar
• Column Inner Diameter (ID): 16–50 mm in our shop, more on request
• Column Brands: Eurospher, Eurospher II
• Safe Investment: Robustness and durability based on superior technology
• Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
• Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available

KNAUER: KNAUER Preparative HPLC Columns

Preparative HPLC columns can be offered with two different hardware types:

• Versatile standard flanged hardware for nearly any application task in batch chromatography
• dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes