KNAUER Eurospher II 100 NH2 Prep HPLC Columns for NP & HILIC (USP L8)

Amino columns for Normal Phase and for HILIC applications (USP L8)

Amino (NH₂) bonded silica gel phases can be used in the normal phase (NP) and HILIC mode, but also for reversed phase (RP) mode. They show alternative normal phase selectivity to unbonded silica, especially for aromatics. Furthermore, the selectivity is influenced by hydrogen bond interactions with the analyte. Amino columns can also be used in the HILIC mode for carbohydrate analysis and for other polar compounds. Their weak anion-exchange properties can be used in the analysis of anions and organic acids.

The amino group is bonded to the silica support via a propyl linker.

Properties

• Separation mechanism: Hydrophilic and ionic interactions
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Amino modification (Aminopropyl)
• 4 % carbon content (without endcapping)

Key features

• Multi mode column for RP, NP, HILIC and IC, Aminopropyl modification without endcapping, outstanding mechanical and chemical stability.

Recommended application areas

• Most flexible phase in the Eurospher II family; can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger); different selectivity to the silica packing; in reversed phase mode mainly used for analysis of carbohydrates. 

KNAUER: NH2

Tip: A precolumn can help to protect your analytical column. Especially when working with highly matrix afflicted samples it is highly recommended to use precolumns.

Stationary phase Eurospher II 100 NH2 is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 NH2 Preparative Standard Columns are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 20, 30, 50
• Particle size (µm): 5, 10, 15

Eurospher II 100 NH2 Preparative dAX Columns with dynamic axial compression are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 25, 30, 50
• Particle size (µm): 5, 10, 15

KNAUER Preparative HPLC Columns

The right choice for all dimensions from semi-prep to batch chromatography to SMB.

• Modifications: Reversed Phase, HILIC, Normale Phase
• Max. Pressure: 400–600 bar
• Column Inner Diameter (ID): 16–50 mm in our shop, more on request
• Column Brands: Eurospher, Eurospher II
• Safe Investment: Robustness and durability based on superior technology
• Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
• Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available

KNAUER: KNAUER Preparative HPLC Columns

Preparative HPLC columns can be offered with two different hardware types:

• Versatile standard flanged hardware for nearly any application task in batch chromatography
• dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes